CN211546518U

CN211546518U - Online filter device

Info

Publication number: CN211546518U
Application number: CN201922400838.0U
Authority: CN
Inventors: 刘东旭
Original assignee: Wuhan Pharmaxab Biological Pharmaceutical Technology Co ltd
Current assignee: Wuhan Pharmaxab Biological Pharmaceutical Technology Co ltd
Priority date: 2019-12-27
Filing date: 2019-12-27
Publication date: 2020-09-22
Anticipated expiration: 2029-12-27

Abstract

The utility model provides an online filtering device, which comprises a suction device, wherein a suction port of the suction device is connected with a distribution three-way check valve, the distribution three-way check valve is provided with three connecting ports, one through connecting port is connected with the suction port, one connecting port is provided with a liquid inlet check valve which is communicated with a suction pipe, and the other connecting port is provided with a liquid discharge check valve which is communicated with a distribution pipe; a filtering device is arranged on the pipeline between the suction pipe and the liquid inlet one-way valve. The utility model discloses can realize filtering and the effect that the distribution returned the liquid container at same online station, and power drive and control are all comparatively convenient. Is particularly suitable for filtering liquid in a centrifugal tube in a centrifugal device. For example, automated cell separation operations are performed using a centrifuge device.

Description

Online filter device

Technical Field

The utility model relates to a cell separation field, especially a be used for online filter equipment of tissue source nature cell.

Background

In the field of cell therapy, the representative methods include "cell immunotherapy" and "stem cell therapy", in which cell separation and culture steps are required, and in the prior art, manual operation is usually adopted in the cell separation process. However, the manual operation has the disadvantages of low efficiency, high labor intensity, high infection probability, high error rate, difficult quality guarantee and the like. The manner of manual operation may affect the final therapeutic effect. In the field of cell detection, and also in the process of drug sensitivity test and detection of solid tumor tissues, the solid tumor tissues also need to be separated into cells for culture, and then the tumor cells are analyzed and checked. These procedures are also usually performed manually, and have the disadvantages of long separation time, high contamination rate, poor cell activity, etc. The above problems also exist in the field of cell storage, such as storage of umbilical cord stem cells. CN110358673A describes a cell separation system and method, but in this scheme, the filtering operation is complicated, resulting in low throughput and efficiency.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the technical problem that an online filter equipment is provided can adopt standardized accessory, and is easy and simple to handle, can further simplify the flow, raise the efficiency.

In order to solve the technical problem, the utility model discloses the technical scheme who adopts is: an online filtering device comprises a suction device, wherein a suction port of the suction device is connected with a distribution three-way one-way valve, the distribution three-way one-way valve is provided with three connecting ports, one through connecting port is connected with the suction port, one connecting port is provided with a liquid inlet one-way valve which is communicated with a suction pipe, and the other connecting port is provided with a liquid discharge one-way valve which is communicated with a distribution pipe;

a filtering device is arranged on the pipeline between the suction pipe and the liquid inlet one-way valve.

In a preferred scheme, the suction pipe is connected with the suction lifting device and is communicated with the liquid inlet one-way valve through a hose, so that the suction pipe can extend into the liquid container and can be lifted up from the liquid container.

In a preferred embodiment, the suction pipette and the dispensing tube are connected to the same liquid container.

In the preferred scheme, the liquid container be centrifugal device's centrifuging tube, the export of distributing pipe is located the centrifuging tube and stops the back, centrifuging tube mouth of pipe's top.

In a preferred embodiment, the suction lifting device is configured to: the lifting and conveying mechanism of the suction lifting device is fixedly connected with a suction cross rod which is fixedly connected with a suction pipe so as to drive the suction pipe to lift.

In a preferred embodiment, the structure of the filtering device is: connectors are arranged at two ends of the sealed cavity, and a filter screen is arranged between the connectors in the sealed cavity.

The seal chamber body is connected by two detachable parts in a sleeved mode, and the filter screen is arranged at the position of the sleeved connection, so that the filter screens with different meshes can be replaced conveniently.

The filter screen is a steel wire filter screen or a polyurethane filter screen.

In a preferred scheme, the structure of the suction device is as follows: the suction injector is connected with the suction screw-nut mechanism, a suction port of a piston sleeve of the suction injector is communicated with a through connection port of the distribution three-way check valve, and the piston sleeve is fixedly arranged on a fixed stop block of the suction screw-nut mechanism so as to limit the axial movement of the piston sleeve;

the suction injector piston rod is movably arranged in the piston sleeve, the free end of the piston rod is fixedly connected with a sliding stop block, the sliding stop block is connected with a nut of a suction feed screw nut mechanism, and a feed screw of the suction feed screw nut mechanism is connected with a suction motor so as to drive the piston rod to reciprocate along the axial direction through the suction motor.

In a preferred scheme, the structure of the suction device is as follows: the piston rod of the suction injector is movably arranged in the piston sleeve, a spring is arranged between the free end head of the piston rod and the piston sleeve, the upper part of the piston sleeve is provided with a rod cavity communicated with the pressure medium pipe, and the suction port of the piston sleeve is communicated with the straight-through connecting port of the distribution three-way one-way valve.

The utility model provides an online filter equipment can realize filtering and the distribution returns the effect of liquid container at same online station, and power drive and control are all comparatively convenient. Is particularly suitable for filtering liquid in a centrifugal tube in a centrifugal device. For example, automated cell separation operations are performed using a centrifuge device.

Drawings

The invention will be further explained with reference to the following figures and examples:

fig. 1 is a schematic front view of the present invention.

Fig. 2 is a schematic top view of the present invention in practical use.

Fig. 3 is a schematic structural diagram of the preferred embodiment of the present invention.

In the figure: the device comprises a centrifugal device 1, a centrifugal tube 2, a crushing device 3, a liquid adding device 4, a first liquid injector 4A, a second liquid injector 4B, a third liquid injector 4C, a fourth liquid injector 4D, a waste liquid discharge device 5, a distribution three-way one-way valve 6, a liquid inlet one-way valve 61, a liquid discharge one-way valve 62, a suction filtering device 7, a piston sleeve 701, a pressure medium tube 702, a spring 703, a suction lifting device 71, a suction cross rod 72, a suction tube 73, a distribution tube 74, a suction injector 75, a suction screw-nut mechanism 76, a suction motor 77, a fixed stop 78, a sliding stop 79, an upper tube device 8, a filtering device 9, a filter screen 91 and a waste liquid receiving bottle 10.

Detailed Description

As shown in fig. 1, an on-line filtering device comprises a suction device, wherein a suction port of the suction device is connected with a distribution three-way one-way valve 6, the distribution three-way one-way valve 6 is provided with three connecting ports, one straight-through connecting port is connected with the suction port, one connecting port is provided with a liquid inlet one-way valve 61 and is communicated with a suction pipe 73, and the other connecting port is provided with a liquid discharge one-way valve 62 and is communicated with a distribution pipe 74;

a filtering device 9 is arranged on a pipeline between the suction pipe 73 and the liquid inlet one-way valve 61. With the structure, the filtering operation can be completed by utilizing one liquid container at one station.

In a preferred scheme, the suction pipe 73 is connected with the suction lifting device 71, and the suction pipe 73 is communicated with the liquid inlet one-way valve 61 through a hose, so that the suction pipe 73 can extend into the liquid container and can be lifted from the liquid container.

Preferably, as shown in fig. 2, the suction pipette 73 and the dispensing tube 74 are connected to the same liquid container. By the structure, the filtering operation is completed at one station.

In the preferred embodiment shown in fig. 2, the liquid container is a centrifuge tube 2 of the centrifuge apparatus 1, and the outlet of the distribution tube 74 is located above the nozzle of the centrifuge tube 2 after the centrifuge tube 2 is stopped.

Preferably, as shown in fig. 1, the suction lifting device 71 has a structure that: the lifting and conveying mechanism of the suction lifting device 71 is fixedly connected with a suction cross rod 72, and the suction cross rod 72 is fixedly connected with a suction pipe 73 so as to drive the suction pipe 73 to lift. With the above-described structure, the lifting operation of the suction pipe 73 is performed so that the liquid in the liquid container is completely sucked into the suction device while being filtered.

In a preferred embodiment as shown in fig. 3, the filtering device 9 has a structure that: connectors are arranged at two ends of the sealed cavity, and a filter screen 91 is arranged between the connectors in the sealed cavity. According to the scheme, the structure is greatly simplified.

The seal chamber body be cup jointed by detachable two parts and connect, filter screen 91 sets up the position of cup jointing the connection to change the filter screen of different mesh numbers.

The filter screen 91 is a steel wire filter screen or a polyurethane filter screen.

The preferred scheme is as shown in figure 1, and the structure of the suction device is as follows: the suction injector 75 is connected with a suction screw-nut mechanism 76, the suction port of the piston sleeve of the suction injector 75 is communicated with the through connection port of the distribution three-way check valve 6, and the piston sleeve is fixedly arranged on a fixed stop 78 of the suction screw-nut mechanism 76 so as to limit the axial movement of the piston sleeve;

the piston rod of the suction injector 75 is movably arranged in the piston sleeve, the free end of the piston rod is fixedly connected with a sliding block 79, the sliding block 79 is connected with a nut of a suction screw-nut mechanism 76, and a screw rod of the suction screw-nut mechanism 76 is connected with a suction motor 77 so as to drive the piston rod to reciprocate along the axial direction through the suction motor 77. With this structure, the filtering operation is completed with one suction syringe 75 at one station. The structure is greatly simplified, and the efficiency is improved. The specific control process is that when the centrifuge tube 2 rotates to the position below the suction tube 73, the motor of the suction lifting device 71 rotates, the screw rod drives the nut to descend, the nut is fixedly connected with the suction cross rod 72, the suction tube 73 descends along with the suction cross rod 72, the bottom end of the suction tube 73 is almost contacted with the tube bottom of the centrifuge tube 2, the piston cylinder of the suction injector 75 is fixed by the fixed stop 78 fixedly arranged on the base of the suction screw-nut mechanism 76, the piston rod of the suction injector 75 is fixed with the sliding stop 79 on the nut of the suction screw-nut mechanism 76, the piston rod of the suction injector 75 moves towards the right end in fig. 1 along with the rotation of the suction motor 77, the liquid in the centrifuge tube 2 is sucked into the suction injector 75 through the liquid inlet one-way valve 61 in the filter device 9 and the distribution three-way one-way valve 6, and when the liquid passes through the filter. When the liquid in the centrifuge tube 2 is completely withdrawn, the amount of liquid withdrawal is controlled by the stroke of the suction feed screw nut mechanism 76. The suction motor 77 is reversed, the liquid in the suction syringe 75 enters the distribution tube 74 through the liquid discharge one-way valve 62 in the distribution three-way one-way valve 6 and is then sent back to the centrifuge tube 2 at the current station, and the suction lifting device 71 is lifted at the same time, and the suction tube 73 is pulled out of the centrifuge tube 2. The whole filtering operation is completed.

As another alternative, as shown in fig. 3, the structure of the suction lifting device 71 is omitted for the convenience of viewing fig. 3, and the structure of the suction device is as follows: the piston rod of the suction injector 75 is movably arranged in the piston sleeve 701, a spring 703 is arranged between the free end head of the piston rod and the piston sleeve, the upper part of the piston sleeve is provided with a rod cavity which is communicated with a pressure medium pipe 702, and the suction port of the piston sleeve 701 is communicated with a through connection port of the distribution three-way check valve 6. When the scheme is used, firstly the suction lifting device 71 descends, the suction cross rod 72 drives the suction pipe 73 to enter the centrifuge tube 2, pressure media in the pressure medium tube 702, such as compressed air or purified water, enter the rod cavity of the piston sleeve 701, the piston rod is pushed to descend, air in the rod-free cavity of the piston sleeve 701 is exhausted, and the step can be completed before the centrifuge tube 2 enters the station and stops, so that the processing time is saved. Through the switching-over of solenoid valve, the pressure of the pressure medium in the pressure medium pipe 702 disappears, under the effect of spring 703, the piston rod goes upward, and the liquid in centrifuging tube 2 enters into the no pole intracavity of piston bush 701 through suction straw 73, filter screen 91 and the feed liquor check valve 61 of filter equipment 9, then the pressure medium in the pressure medium pipe 702 enters into the pole chamber of piston bush 701 once more, and the liquid after will filtering is discharged back again in centrifuging tube 2 through flowing back check valve 62, accomplishes filtering operation.

The above embodiments are merely preferred technical solutions of the present invention, and should not be considered as limitations of the present invention, and the features in the embodiments and the examples in the present application may be arbitrarily combined with each other without conflict. The protection scope of the present invention shall be defined by the claims and the technical solutions described in the claims, including the technical features of the equivalent alternatives as the protection scope. Namely, equivalent alterations and modifications within the scope of the invention are also within the scope of the invention.

Claims

1. An online filter device is characterized in that: the device comprises a suction device, wherein a suction port of the suction device is connected with a distribution three-way one-way valve (6), the distribution three-way one-way valve (6) is provided with three connecting ports, one through connecting port is connected with the suction port, one connecting port is provided with a liquid inlet one-way valve (61) which is communicated with a suction pipe (73), and the other connecting port is provided with a liquid discharge one-way valve (62) which is communicated with a distribution pipe (74);

a filtering device (9) is arranged on a pipeline between the suction pipe (73) and the liquid inlet one-way valve (61).

2. An in-line filtration device as claimed in claim 1, wherein: the suction pipe (73) is connected with the suction lifting device (71), and the suction pipe (73) is communicated with the liquid inlet one-way valve (61) through a hose so that the suction pipe (73) can extend into the liquid container and can be lifted up from the liquid container.

3. An in-line filtration device as claimed in claim 2, wherein: the suction pipe (73) and the distribution pipe (74) are communicated with the same liquid container.

4. An in-line filtration device as claimed in claim 2 or 3, wherein: the liquid container be centrifuging tube (2) of centrifugal device (1), the export of distributing pipe (74) is located centrifuging tube (2) and stops the back, centrifuging tube (2) orificial top.

5. An in-line filtration device as claimed in claim 2, wherein: the structure of the suction lifting device (71) is as follows: a synchronous belt mechanism, a gear rack mechanism or a screw rod nut mechanism driven by a motor, or an air cylinder or a hydraulic cylinder driven by a pressure medium, a lifting power transmission mechanism of the suction lifting device (71) is fixedly connected with a suction cross rod (72), and the suction cross rod (72) is fixedly connected with a suction pipe (73) so as to drive the suction pipe (73) to lift.

6. An in-line filtration device as claimed in claim 2, wherein: the structure of the filtering device (9) is as follows: connectors are arranged at two ends of the sealed cavity, and a filter screen (91) is arranged between the connectors in the sealed cavity.

7. An in-line filtration device as claimed in claim 6, wherein: the seal chamber body be cup jointed by detachable two parts and connect, filter screen (91) set up in the position of cup jointing the connection to change the filter screen of different mesh numbers.

8. An in-line filtration device as claimed in claim 6, wherein: the filter screen (91) is a steel wire filter screen or a polyurethane filter screen.

9. An in-line filtration device as claimed in any one of claims 1 to 3 and 5 to 8, wherein: the structure of the suction device is as follows: the suction injector (75) is connected with a suction screw-nut mechanism (76), a suction port of a piston sleeve of the suction injector (75) is communicated with a through connection port of the distribution three-way check valve (6), and the piston sleeve is fixedly arranged on a fixed stop block (78) of the suction screw-nut mechanism (76) so as to limit the axial movement of the piston sleeve;

a piston rod of the suction injector (75) is movably arranged in a piston sleeve, the free end of the piston rod is fixedly connected with a sliding stop block (79), the sliding stop block (79) is connected with a nut of a suction screw-nut mechanism (76), and a screw rod of the suction screw-nut mechanism (76) is connected with a suction motor (77) so as to drive the piston rod to reciprocate along the axial direction through the suction motor (77).

10. An in-line filtration device as claimed in any one of claims 1 to 3 and 5 to 8, wherein: the structure of the suction device is as follows: the piston rod of the suction injector (75) is movably arranged in the piston sleeve (701), a spring is arranged between the free end head of the piston rod and the piston sleeve, the upper part of the piston sleeve is provided with a rod cavity which is communicated with the pressure medium pipe (702), and the suction port of the piston sleeve (701) is communicated with the through connecting port of the distribution three-way check valve (6).