CN212068355U - Novel efficient DNA filters degerming device - Google Patents

Novel efficient DNA filters degerming device Download PDF

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Publication number
CN212068355U
CN212068355U CN202020455582.3U CN202020455582U CN212068355U CN 212068355 U CN212068355 U CN 212068355U CN 202020455582 U CN202020455582 U CN 202020455582U CN 212068355 U CN212068355 U CN 212068355U
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CN
China
Prior art keywords
container
syringe
hose
liquid
air
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Active
Application number
CN202020455582.3U
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Chinese (zh)
Inventor
彭伟
李东旭
叶桦
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Suzhou Jinweizhi Biological Technology Co ltd
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Suzhou Jinweizhi Biological Technology Co ltd
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Priority to CN202020455582.3U priority Critical patent/CN212068355U/en
Priority to PCT/CN2020/132642 priority patent/WO2021196675A1/en
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D29/00Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
    • B01D29/01Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D29/00Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
    • B01D29/76Handling the filter cake in the filter for purposes other than for regenerating
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D61/00Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor

Abstract

The utility model relates to a novel efficient DNA filters degerming device, including pneumatics subassembly, filtering component, atmospheric pressure transmission assembly and control assembly, filtering component is including the first container, filter membrane (14) that hold the liquid of treating to filter and the second container that holds the liquid after straining that from top to bottom set gradually, the pneumatics subassembly pass through atmospheric pressure transmission assembly with first container or second container intercommunication, the pneumatics subassembly is in produce the malleation in the first container or be in produce the negative pressure in the second container, make and treat the liquid warp filter membrane (14) filtration inflow the second container. The utility model discloses combine filter membrane and traditional hand propelled syringe with the pneumatics system together, formed the automatic DNA filtration bacteria removing device of easy equipment easy separation, longe-lived, process technology, under the prerequisite of guaranteeing plasmid DNA quality, realized the automation of crossing the membrane degerming.

Description

Novel efficient DNA filters degerming device
Technical Field
The application relates to a filter equipment especially relates to a novel efficient DNA filters bacteria removing device.
Background
In the existing sterilization method, the filter membrane filtration is the first choice due to the simple operation flow and good sterilization effect, but the facility method used with the filter membrane is not selected so much. Although the traditional hand-push type syringe (or syringe type filter) is simple to operate, the batch production of the sterile plasmids cannot be completed at all, the production cost is greatly increased due to the two reasons of long time consumption and large demand on manpower, and the production efficiency cannot meet the production demand. The suction filtration system should be operated due to the drawback of low efficiency of the conventional syringe (or syringe filter). Unfortunately, the suction filtration system only plays a role in the extraction of plasmids, and has no obvious contribution to the mass production of sterile plasmids (we found in experiments that plasmids can hardly pass through the filter membrane under the condition of negative pressure suction filtration); the filtration system realizes automation in operation process, but the recovery efficiency of plasmids after membrane filtration is too low, and the yield is too low.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is: aiming at solving the defects of the filter membrane filtration facility method in the prior art, a novel high-efficiency DNA filtration and sterilization device is provided.
The utility model provides a technical scheme that its technical problem adopted is:
the utility model provides a novel efficient DNA filters degerming device, includes air compression subassembly, filtering component, atmospheric pressure transmission assembly and control assembly, filtering component is including the first container, the filter membrane that hold the liquid of treating to filter and the second container that holds the liquid after straining that from top to bottom set gradually, the air compression subassembly pass through atmospheric pressure transmission assembly with first container or second container intercommunication, the air compression subassembly is in produce the malleation in the first container or be in produce the negative pressure in the second container, make and treat the filtered liquid warp the filter membrane filters the inflow the second container.
In one embodiment, the first container or the second container is a syringe (12), a piston (13) is arranged in an empty barrel of the syringe (12), and the piston (13) slides along the empty barrel of the syringe (12) and seals a liquid space in the empty barrel.
In one embodiment, the first container is a syringe (12), the second container is a centrifuge tube (15), the filter membrane is arranged at the nipple end of the syringe, the gas output by the air pressure assembly is transmitted to the piston end of the syringe through the air pressure transmission assembly and pushes the piston to press downwards, the piston presses the liquid to be filtered in the syringe to flow out from the nipple end, and the liquid to be filtered is collected by the centrifuge tube after being filtered by the filter membrane.
In one embodiment, the air compressing assembly includes an air compressor having an air flow input port and an air flow output port.
In one embodiment, the air pressure transmission assembly comprises a hose and a threaded screw cap, the threaded screw cap is fixed at the piston end of the injector and seals the inner cylinder of the injector, a vent hole is formed in the threaded screw cap, one end of the hose is connected with an air flow output port of the air compressor, and the other end of the hose is connected with the vent hole.
In one embodiment, the hose is a transparent plastic hose with the pipe diameter of 0.5-0.7 mm.
In one embodiment, the hose is connected with a pressure gauge for detecting the air pressure in the hose.
In one embodiment, the control assembly comprises a waterproof junction box, an intermediate relay and a relay switch, wherein the intermediate relay and the relay switch are arranged in the waterproof junction box, the intermediate relay is arranged on the hose, and the relay switch is opened or closed to control the on or off of air flow in the hose through the intermediate relay.
In one embodiment, the control assembly comprises a relay power line and an air compressor power line, two ends of the relay power line are respectively connected with the relay switch and a first power plug, two ends of the air compressor power line are respectively connected with the air compressor and a second power plug, and the first power plug and the second power plug are respectively connected with or disconnected from an external power supply.
In one embodiment, the syringe is a threaded needle syringe and the piston is a rubber plug.
The utility model has the advantages that: the utility model discloses combine filter membrane and traditional hand propelled syringe with the pneumatics system and be in the same place, formed the automatic DNA filtration bacteria removing device of easy equipment readily releasable, area is little, longe-lived, process technology, under the prerequisite of guaranteeing plasmid DNA quality, realized that the membrane degerming is automatic, reduced the cost of labor, improved production efficiency.
Drawings
The technical solution of the present application is further explained below with reference to the drawings and the embodiments.
FIG. 1 is a schematic structural diagram of a novel and efficient DNA filtration and sterilization apparatus according to an embodiment of the present application.
1 an external power supply;
2 a plug;
3, conducting wires;
4, an air compressor;
5, a hose;
7, a waterproof junction box;
8, a power switch;
9 an intermediate relay;
10 relay switch;
11, screwing a cover;
12 an injector;
13 a piston;
14, filtering the membrane;
15 centrifuging the tube;
16 a centrifuge tube rack;
17 a support frame is arranged.
Detailed Description
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict.
In the description of the present application, it is to be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientation or positional relationship indicated in the drawings for convenience in describing the present application and for simplicity in description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed in a particular orientation, and be operated in a particular manner, and are not to be considered limiting of the scope of the present application. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the invention, unless otherwise specified, "a plurality" means two or more.
In the description of the present application, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art through specific situations.
The technical solutions of the present application will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.
The utility model provides a novel efficient DNA filters degerming device, includes air compression subassembly, filtering component, atmospheric pressure transmission subassembly and control assembly, and filtering component is including the first container that holds treating filterable liquid, filter membrane 14 and the second container that holds receiving and receiving liquid behind the nanofiltration that from top to bottom sets gradually, and the air compression subassembly passes through atmospheric pressure transmission subassembly and first container or second container intercommunication. The air pressure assembly creates a positive pressure in the first container or a negative pressure in the second container, causing the liquid to be filtered to flow through the filter membrane 14 into the second container. The filter membrane filtration treatment is a solid-liquid separation technology, and is a technology for filtering liquid through membrane pores and intercepting impurities in the liquid without chemical changes. The filter sterilization device of this embodiment uses physical retention method to remove bacteria from liquid or air for the purpose of sterility, and the filter membrane 14 commonly used in biology has two pore sizes of 0.45 μm and 0.22 μm.
In one embodiment, the first or second container is a syringe 12, and a plunger 13 is disposed in an empty barrel of the syringe 12, the plunger 13 sliding along the empty barrel of the syringe 12 and sealing a fluid space in the empty barrel. The syringe 12 is a conventional medical device, and the barrel (and its empty barrel containing the liquid) may be plastic or glass, and is typically provided with a graduated indicator of the volume of liquid in the syringe 12. The syringe 12 in this embodiment retains the piston 13 and simultaneously cancels the piston shaft and the piston handle of the general syringe 12, and the function of pushing the piston 13 is realized by converting the original pushing by hand into the pushing by gas in the embodiment, which also enables the pushing action of the piston 13 to be performed in a more uniform amplitude, so as to improve the filtering precision. The piston 13 can not only push liquid for filtration, but also seal the empty barrel and prevent the liquid from flowing back.
In one embodiment, the first container is syringe 12 and the second container is centrifuge tube 15, i.e., the filter assembly includes syringe 12, filter membrane 14 and centrifuge tube 15 arranged in a top-down order, and syringe 12 and filter membrane 14 may be respectively supported by a stand to maintain a relatively fixed position, such as centrifuge tube stand 16 to hold centrifuge tube 15 vertically, device stand 17 to hold syringe 12 vertically with the nipple end of syringe 12 aligned with the opening of centrifuge tube 15 and to maintain filter membrane 14 therebetween. The device holder 17 may be a cylindrical solid with a circular hole therein adapted to the barrel of the syringe 12 to vertically secure the syringe 12. The nipple end of syringe 12 is located to filter membrane 14, and the gaseous piston end that carries to syringe 12 and promote piston 13 to push down through atmospheric pressure transmission assembly of pneumatics subassembly output, and piston 13 oppresses treating in the syringe 12 and filters liquid and flow out by the nipple end, treats that filtering liquid is collected by centrifuging tube 15 after filter membrane 14 filters, accomplishes and filters. The centrifuge tube 15 is a tubular sample container, and in one embodiment, the centrifuge tube 15 is a conical centrifuge tube with a conical bottom and an open top, so as to collect filtered liquid.
In one embodiment, the air compressor assembly includes an air compressor 4. The air compressor 4 is a general air compressor, and is a main body of an air source device, and is a device for converting mechanical energy of a prime mover (usually, an electric motor) into gas pressure energy, and is also an air pressure generating device for compressing air. The air compressor machine 4 in this embodiment is provided with an airflow input port and an airflow output port respectively, the airflow input port is communicated with the atmosphere, and the airflow output port conveys air to the air pressure transmission assembly.
In one embodiment, the pneumatic transfer assembly comprises a hose 5 and a screw cap 11, the screw cap 11 being secured to the plunger end of the syringe 12 and sealing the inner barrel of the syringe 12. The threaded screw cap 11 is provided with an air vent, one end of the hose 5 is connected with an airflow output port of the air compressor 4, and the other end of the hose is connected with the air vent. The screw cap 11 is fixed to and vented to one end of the hose 5 to facilitate connection and disconnection of the hose 5 to the syringe 12 by screwing the screw cap 11 to the syringe 12.
In order to improve the screw connection effect, a screw corresponding to the screw of the screw cap 11 may be formed at a corresponding position of the syringe 12. In one embodiment, the syringe 12 is a screw-type needle syringe, and the top end of the syringe 12 is threaded to form a detachable threaded connection with the threaded cap 11. The piston 13 in the syringe 12 may be a rubber plug.
Due to the movable connection requirements of the air compression assembly, the filtering assembly, the air pressure transmission assembly, the control assembly and the like, in one embodiment, the hose 5 is a transparent plastic hose, the transparent pipe body can facilitate the condition in the pipe, and accidental factors such as back suction and impurities can be eliminated. In one embodiment, the tube 5 has a diameter of 0.5-0.7mm, such as 0.5mm or 0.7mm, and preferably, a 0.6mm transparent tube is used.
In one embodiment, the hose 5 is connected with a pressure gauge 6 for detecting the air pressure in the hose 5, the air pressure condition in the device system, especially the air pressure condition at the output end of the air compressor, is judged according to the reading of the pressure gauge, the excessive or excessively low air pressure is prevented, the air pressure fluctuation is monitored, the stable filtering progress is kept, and the filtering precision is improved. In one embodiment, the pressure gauge 6 is a gas-oil separation pressure gauge.
In one embodiment, the control assembly comprises a waterproof junction box 7, an intermediate relay 9 and a relay switch 10, wherein the intermediate relay 9 and the relay switch 10 are arranged in the waterproof junction box 7, the intermediate relay 9 is arranged on the hose 5, and the relay switch 10 is opened or closed to control the on-off of the intermediate relay 9, so that the on-off of the air flow in the hose 5 is controlled through the intermediate relay 9. A relay is an electric control device that generates a predetermined step change in a controlled amount in an electric output circuit when a change in an input amount meets a predetermined requirement. In this embodiment, the intermediate relay 9 transmits an intermediate signal in the control circuit, which may be used to switch or regulate the flow of air in the hose 5 by closing an electrically operated valve on the connecting hose. The waterproof junction box 7 can be a plastic box body, two ends of the waterproof junction box are respectively provided with an opening for passing through the hose 5, and the opening can be provided with a sealing rubber ring for covering the hose 5.
In one embodiment, the control assembly further includes a relay power line and an air compressor power line, or collectively, a lead 3. Two ends of a relay power line are respectively connected with the relay switch 10 and a first power plug, two ends of an air compressor power line are respectively connected with the air compressor 4 and a second power plug, and the first power plug and the second power plug are respectively used for connecting or disconnecting the external power supply 1. The external power supply 1 is communicated with the rear part through an air compressor power line to enable the air compressor 4 to work, and the relay switch 10 is communicated with the rear part through a relay power line to enable the relay switch to work.
The control assembly accessible connects plug 2 and the 3 controls of wire of inserting of difference the utility model discloses filter sterilization device's work forms a plurality of on-off control of difference. In order to improve the efficiency of the control assembly, in one embodiment, a power switch 8 is further disposed in the waterproof junction box 7, and the power switch 8 is connected to the relay power line to control the on/off of the current in the relay power line, thereby serving as a front switch of the relay switch 10. When the relay switch 10 is included, the present embodiment has three switch control methods, such as the plug 2, the power switch 8, and the relay switch 10, and each part is controlled by an independent switch, so that the air flow is switched on and off in real time, the control accuracy is improved, the safety and the accuracy of filtration are ensured, and particularly when the components, such as the flow of the pressure gauge 6 or the reading of air pressure data, are combined.
In one embodiment, the novel high-efficiency DNA filter sterilization device comprises the following processes: the gas produced by the air compressor 4 is connected to an oil-water separation type gas pressure gauge by a transparent hose 5 and then communicated to a waterproof junction box 7 containing an independent control switch. After the relay switch 10 is turned on, the gas is discharged through the intermediate relay 9. The gas then pushes the liquid down through a threaded needle syringe containing a rubber plug 13 sealed by a threaded cap 11. The liquid is discharged through the filter 14 and collected in a centrifuge tube 15. Centrifuge tube 15 is placed on centrifuge tube rack 16 and several other filter assemblies are held by assembly support bracket 17.
The utility model has the advantages that: the utility model discloses combine filter membrane and traditional hand propelled syringe with the pneumatics system and be in the same place, formed the automatic DNA filtration bacteria removing device of easy equipment readily releasable, area is little, longe-lived, process technology, under the prerequisite of guaranteeing plasmid DNA quality, realized that the membrane degerming is automatic, reduced the cost of labor, improved production efficiency.
In light of the foregoing description of the preferred embodiments according to the present application, it is to be understood that various changes and modifications may be made without departing from the spirit and scope of the invention. The technical scope of the present application is not limited to the contents of the specification, and must be determined according to the scope of the claims.

Claims (10)

1. The utility model provides a novel efficient DNA filters bacteria removing device, its characterized in that includes pneumatics subassembly, filtering component, atmospheric pressure transmission assembly and control assembly, filtering component is including the first container, filter membrane (14) that hold the liquid of treating to filter and the second container that holds the liquid after straining that from top to bottom set gradually, the pneumatics subassembly pass through atmospheric pressure transmission assembly with first container or second container intercommunication, the pneumatics subassembly is in produce the malleation in the first container or be in produce the negative pressure in the second container, make the liquid of treating to filter the process filter membrane (14) filters the inflow the second container.
2. The novel and efficient DNA filtration sterilization apparatus according to claim 1, wherein the first container or the second container is a syringe (12), a piston (13) is arranged in the empty barrel of the syringe (12), and the piston (13) slides along the empty barrel of the syringe (12) and seals the liquid space in the empty barrel.
3. The novel and efficient DNA filtration and sterilization device according to claim 2, wherein the first container is a syringe (12), the second container is a centrifuge tube (15), the filter membrane (14) is disposed at the nipple end of the syringe (12), the air output from the air pressure assembly is transmitted to the piston end of the syringe (12) through the air pressure transmission assembly and pushes the piston (13) to press down, the piston (13) presses the liquid to be filtered in the syringe (12) to flow out from the nipple end, and the liquid to be filtered is collected by the centrifuge tube (15) after being filtered by the filter membrane (14).
4. The novel high-efficiency DNA filtration sterilization apparatus according to claim 3, wherein said air compressor assembly comprises an air compressor (4), said air compressor (4) is provided with an air inlet and an air outlet respectively.
5. The novel high-efficiency DNA filtration sterilization device according to claim 4, wherein the pneumatic transmission assembly comprises a hose (5) and a screw cap (11), the screw cap (11) is fixed at the piston end of the syringe (12) and seals the inner cylinder of the syringe (12), the screw cap (11) is provided with a vent hole, one end of the hose (5) is connected with the airflow output port of the air compressor (4), and the other end of the hose is connected with the vent hole.
6. The novel high-efficiency DNA filtration sterilization apparatus according to claim 5, wherein the hose (5) is a transparent plastic hose with a diameter of 0.5-0.7 mm.
7. The novel and efficient DNA filtration and sterilization apparatus according to claim 5, wherein a pressure gauge (6) for detecting the air pressure in the hose (5) is connected to the hose (5).
8. The novel high-efficiency DNA filtration sterilization apparatus according to claim 5, wherein the control assembly comprises a waterproof junction box and an intermediate relay (9) and a relay switch (10) arranged in the waterproof junction box, the intermediate relay (9) is arranged on the hose (5), and the relay switch (10) is opened or closed to control the on or off of the air flow in the hose (5) passing through the intermediate relay (9).
9. The novel and efficient DNA filtration sterilization device according to claim 8, wherein the control assembly further comprises a relay power line and an air compressor power line, the two ends of the relay power line are respectively connected with the relay switch (10) and the first power plug, the two ends of the air compressor power line are respectively connected with the air compressor (4) and the second power plug, and the first power plug and the second power plug are respectively connected with or disconnected from the external power supply (1).
10. The new and efficient DNA filtration sterilization apparatus according to claim 3, wherein said syringe (12) is a screw-type needle syringe and said piston (13) is a rubber plug.
CN202020455582.3U 2020-04-01 2020-04-01 Novel efficient DNA filters degerming device Active CN212068355U (en)

Priority Applications (2)

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CN202020455582.3U CN212068355U (en) 2020-04-01 2020-04-01 Novel efficient DNA filters degerming device
PCT/CN2020/132642 WO2021196675A1 (en) 2020-04-01 2020-11-30 Dna filtering and sterilizing device

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Application Number Priority Date Filing Date Title
CN202020455582.3U CN212068355U (en) 2020-04-01 2020-04-01 Novel efficient DNA filters degerming device

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WO (1) WO2021196675A1 (en)

Cited By (1)

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Publication number Priority date Publication date Assignee Title
CN113332790A (en) * 2021-05-28 2021-09-03 暨南大学 Novel sample filter

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