CN213708371U

CN213708371U - Biological enzyme's separation and purification device

Info

Publication number: CN213708371U
Application number: CN202022371071.6U
Authority: CN
Inventors: 陈贵元; 刘涵; 李岩; 李倩倩
Original assignee: Dali University
Current assignee: Dali University
Priority date: 2020-10-22
Filing date: 2020-10-22
Publication date: 2021-07-16
Anticipated expiration: 2030-10-22

Abstract

The utility model discloses a separation and purification device of biological enzyme, which comprises a base, wherein the upper surface of the base is respectively provided with a liquid storage tank, a centrifuge and a salting-out tank from left to right, a top cover is arranged at the upper end of the liquid storage tank, a motor box is arranged inside the top cover, a motor is arranged in the motor box, the output end of the motor is provided with a rotating rod, the lower end of the rotating rod penetrates through the lower side wall of the top cover and extends into the liquid storage tank, the user lifts the pull rod upwards, after the pull rod is lifted, the liquid in the second liquid guide pipe flows into the outer shell through the circular hole arranged at the lower end of the side wall of the inner shell, and flows into the liquid collecting bottle from the liquid collecting pipe, a user can control the number of round holes for communicating the inner shell and the outer shell by lifting the height of the pull rod, thereby the outflow of control liquid for the sample process is convenient, sanitary, need not to open purification device alright carry out the liquid sample and control and get the volume.

Description

Biological enzyme's separation and purification device

Technical Field

The utility model relates to a bioengineering technical field, the concrete field is a separation and purification device of biological enzyme.

Background

Biological enzymes are organic substances with catalytic action produced by living cells, most of which are proteins, and few of which are RNAs, and at present, the fields of manufacture and application of biological enzymes are gradually expanded, so that the biological enzymes are better contributed to the progress of human science industry, people need to purify the biological enzymes, and the purification and separation process is a process of purifying and separating a certain mixture into two or more products with different compositions by means of physical, chemical or biological means or combination of the methods. In general, say exactly to separate out some kind or some kind of material from complicated mixture, purification and separation process runs through in whole production technology process, is the important means of obtaining final product, need constantly extract and test the test solution at the in-process that biological enzyme carries out the purification, and the extraction process of current device is more troublesome, mostly need open purification device and artifical manual extraction for the test solution in the purification process receives external pollution very easily and be difficult to control the sample volume of getting.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a separation and purification device of biological enzyme to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a separation and purification device of biological enzyme comprises a base, wherein a liquid storage tank, a centrifugal machine and a salting-out tank are respectively arranged on the upper surface of the base from left to right, a top cover is arranged at the upper end of the liquid storage tank, a motor box is arranged in the top cover, a motor is arranged in the motor box, a rotating rod is arranged at the output end of the motor, the lower end of the rotating rod penetrates through the lower side wall of the top cover and extends into the liquid storage tank, stirring blades are arranged at the lower end of the rotating rod, a liquid outlet is arranged at the lower end of the right side wall of the liquid storage tank, a first liquid conveying pump is arranged on the right side of the liquid outlet, support frames are respectively arranged at the left end and the right end of the centrifugal machine, a filter tank is arranged at the upper ends of the two support frames, a filter membrane fixing plate is clamped in the filter tank through a, the output end of the first liquid delivery pump is provided with one end of a first liquid guide pipe, the other end of the first liquid guide pipe is communicated with the inner cavity of the filter box, the lower side wall of the filter box is provided with a liquid outlet, the liquid outlet is connected with one end of the guide pipe, the other end of the guide pipe is connected with the inner cavity of the centrifugal machine, the guide pipe is provided with a control valve, the lower end of the right side wall of the centrifugal machine is communicated with one end of a second liquid guide pipe, the other end of the second liquid guide pipe is connected with the input end of the second liquid delivery pump, the output end of the second liquid delivery pump is connected with one end of a third liquid guide pipe, the other end of the third liquid guide pipe is connected with a salting-out box, the upper pipe wall of the second liquid guide pipe is provided with a liquid taking mechanism, the liquid taking mechanism comprises an outer shell, the inner shell, the utility model discloses a liquid storage tank, including casing, shell body, casing, inner shell, outer shell, inner shell, outer shell, inner shell, outer shell, inner shell.

Preferably, the upper surface of the top cover is provided with a pull ring.

Preferably, the upper end of the filter membrane tank is in threaded connection with a membrane pressing cover, and the upper surface of the membrane pressing cover is provided with a sanding block.

Preferably, a diversion trench is arranged on the outer side of the liquid injection port.

Preferably, the contact position of the liquid storage tank and the top cover is sealed.

Compared with the prior art, the beneficial effects of the utility model are that: the utility model provides a separation and purification device of bio-enzyme, the utility model discloses when the sample, the user upwards lifts the pull rod, and the round hole that the interior liquid of second drain pipe set up through interior casing lateral wall lower extreme flows into inside the shell body after the pull rod is lifted to flow into the collecting tank from the collecting tank, the round hole quantity of casing and shell body in highly controlling the intercommunication of user's accessible mentioning the pull rod, thereby the outflow of control liquid, make sampling process convenient, the health, need not to open purification device alright carry out the liquid sample and control the volume of getting, the utility model discloses set up electronic stirring fan blade in the top cap, stirring fan blade can together dismantle together with the top cap, has made things convenient for the washing of liquid reserve.

Drawings

FIG. 1 is a schematic sectional view of the front view of the present invention;

FIG. 2 is a schematic view of the filter membrane fixing plate of the present invention;

fig. 3 is a schematic top view of the film pressing cover of the present invention;

fig. 4 is an enlarged schematic view of a portion a in fig. 1.

In the figure: 1-base, 2-liquid storage tank, 3-centrifuge, 4-salting-out tank, 5-top cover, 6-motor tank, 7-motor, 8-rotating rod, 9-stirring fan blade, 10-first infusion pump, 11-support frame, 12-filter tank, 13-filter membrane fixing plate, 14-filter membrane tank, 15-first liquid guide pipe, 16-guide pipe, 17-control valve, 18-second liquid guide pipe, 19-second infusion pump, 20-third liquid guide pipe, 21-outer shell, 22-inner shell, 23-rubber plug, 24-pull rod, 25-liquid collection pipe, 26-liquid collection bottle, 27-liquid injection port, 28-pull ring and 29-pressure membrane cover.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides a technical solution: a separation and purification device of biological enzyme comprises a base 1, wherein a liquid storage tank 2, a centrifugal machine 3 and a salting-out tank 4 are respectively arranged on the upper surface of the base 1 from left to right, a top cover 5 is arranged at the upper end of the liquid storage tank 2, a motor tank 6 is arranged inside the top cover 5, a motor 7 is arranged in the motor tank 6, a rotating rod 8 is arranged at the output end of the motor 7, the lower end of the rotating rod 8 penetrates through the lower side wall of the top cover 5 and extends into the liquid storage tank 2, stirring fan blades 9 are arranged at the lower end of the rotating rod 8, a liquid outlet is arranged at the lower end of the right side wall of the liquid storage tank 2, a first liquid conveying pump 10 is arranged at the right side of the liquid outlet, support frames 11 are respectively arranged at the left end and the right end of the centrifugal machine 3, a filter tank 12 is arranged at the upper ends of the two support frames 11, a filter membrane fixing plate 13 is clamped inside the filter tank 12 through clamping grooves, the output end of the first liquid delivery pump 10 is provided with one end of a first liquid guide pipe 15, the other end of the first liquid guide pipe 15 is communicated with the inner cavity of the filter box 12, the lower side wall of the filter box 12 is provided with a liquid outlet and the liquid outlet is connected with one end of a guide pipe 16, the other end of the guide pipe 16 is connected with the inner cavity of the centrifuge 3 and the guide pipe 16 is provided with a control valve 17, a worker can control the amount of liquid discharged into the centrifuge 3 through the control valve 17 arranged on the guide pipe 16, the lower end of the right side wall of the centrifuge 3 is communicated with one end of a second liquid guide pipe 18 and the other end of the second liquid guide pipe 18 is connected with the input end of a second liquid delivery pump 19, the output end of the second liquid delivery pump 19 is connected with one end of a third liquid guide pipe 20, the other end of the third liquid guide pipe 20 is connected with the salting-, get liquid mechanism and include shell body 21, the inside of shell body 21 and be in the lateral wall is equipped with interior casing 22 on second catheter 18, the lower extreme of interior casing 22 with the inside of second catheter 18 is linked together, the lateral wall lower extreme of interior casing 22 is equipped with the round hole, the inside of interior casing 22 is equipped with rubber buffer 23 just the upper end of rubber buffer 23 is equipped with the one end of pull rod 24, the other end of pull rod 24 runs through interior casing 22 with the lateral wall is gone up to shell body 21, shell body 21 with interior casing 22 is the transparent material casing, the right side wall upper end of shell body 21 is equipped with the one end of collector tube 25 and the other end threaded connection of collector tube 25 has a collector bottle 26, upwards mentions pull rod 24, after pull rod 24 is lifted, liquid in the second catheter 18 flows into through the round hole that interior casing 22 lateral wall lower extreme set up inside shell body 21, and flow into the liquid collecting bottle 26 from the liquid collecting pipe 25, the side wall of the liquid collecting bottle 26 is provided with scale marks, so that a user can observe the amount of a sample, the number of round holes for communicating the inner shell 22 with the outer shell 21 is controlled by lifting the height of the pull rod 24, the outflow speed and the outflow quantity of liquid are controlled, the sampling process is convenient and sanitary, after a sample with a proper amount is taken out, the liquid collecting bottle 26 only needs to be screwed off in a rotating mode, the user does not need to hold the liquid collecting bottle 26 in hand during sampling, and the right end of the upper side wall of the salting-out box 4 is provided with a liquid injection port 27.

Specifically, the top cover 5 is provided with a pull ring 28 on the upper surface thereof to facilitate the removal and cleaning of the top cover 5.

Specifically, the upper end of the filter membrane tank 14 is connected with a membrane pressing cover 29 in a threaded manner, and the upper surface of the membrane pressing cover 29 is provided with a sanding block, so that the filter membrane in the filter membrane tank 14 can be more stable by the membrane pressing cover 29.

Specifically, a diversion trench is arranged outside the liquid injection port 27 to facilitate the addition of the saline solution.

Specifically, the contact position of the liquid storage tank 2 and the top cover 5 is sealed to prevent the biological enzyme stock solution from overflowing in the stirring process.

The working principle is as follows: when the utility model is used, the operator firstly places the filter membrane into the filter membrane groove 14 of the filter membrane fixing plate 13, then pours the raw liquid of the biological enzyme into the liquid storage tank 2 and starts the motor 7, the output end of the motor 7 drives the rotating rod 8 to rotate so as to drive the stirring fan blades 9 to rotate, so that the raw liquid of the biological enzyme in the liquid storage tank 2 flows, so that the raw liquid of the biological enzyme is fully mixed, then the raw liquid of the biological enzyme is conveyed to the filter tank 12 through the first infusion pump 10, the liquid filtered by the filter membrane in the filter tank 12 is discharged into the centrifuge 3 through the conduit 16, the operator can control the amount of the liquid discharged into the centrifuge 3 through the control valve 17 arranged on the conduit 16, the liquid can be sampled and detected after being discharged from the centrifuge 3, the user lifts the pull rod 24 upwards, the liquid in the second liquid guide pipe 18 after lifting the pull rod 24 flows into the outer shell 21 through the round hole arranged at the lower, and flow into the liquid collecting bottle 26 from the liquid collecting pipe 25, the user can control the number of round holes communicating the inner shell 22 with the outer shell 21 by lifting the height of the pull rod 24, thereby controlling the outflow speed and outflow quantity of the liquid, making the sampling process convenient and sanitary, after taking out a proper amount of samples, the user only needs to rotate and unscrew the liquid collecting bottle 26, then the liquid is conveyed to the salting-out box 4 through the second liquid conveying pump 19, the user injects a salt solution into the salting-out box 4 through the liquid injecting port 27 for salting-out, and finally a semi-finished product of the biological enzyme is prepared for subsequent processing.

In the description of the present invention, unless expressly stated or limited otherwise, the terms "mounted," "connected," and "fixed" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral part; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The utility model discloses the standard part that uses all can purchase from the market, and dysmorphism piece all can be customized according to the record of description and attached drawing, and the concrete connection mode of each part all adopts conventional means such as ripe bolt, rivet, welding among the prior art, and machinery, part and equipment all adopt prior art, and conventional model is including the conventional connected mode of circuit connection adoption conventional among the prior art, and here detailed description is not again.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A separation and purification device of biological enzyme comprises a base (1), and is characterized in that: the upper surface of the base (1) is respectively provided with a liquid storage tank (2), a centrifugal machine (3) and a salting-out tank (4) from left to right, the upper end of the liquid storage tank (2) is provided with a top cover (5), the inside of the top cover (5) is provided with a motor box (6) and a motor (7) is arranged in the motor box (6), the output end of the motor (7) is provided with a rotating rod (8), the lower end of the rotating rod (8) penetrates through the lower side wall of the top cover (5) and extends into the liquid storage tank (2), the lower end of the rotating rod (8) is provided with a stirring fan blade (9), the lower end of the right side wall of the liquid storage tank (2) is provided with a liquid discharge port, the right side of the liquid discharge port is provided with a first infusion pump (10), the left and right ends of the centrifugal machine (3) are respectively provided with support frames (11), the upper ends of the two support frames (11) are provided with filter tanks, the lower side wall of the filter membrane fixing plate (13) is provided with an opening, a filter membrane groove (14) is arranged inside the filter membrane fixing plate (13), the output end of the first infusion pump (10) is provided with one end of a first liquid guide pipe (15), the other end of the first liquid guide pipe (15) is communicated with the inner cavity of the filter box (12), the lower side wall of the filter box (12) is provided with a liquid outlet, the liquid outlet is connected with one end of a guide pipe (16), the other end of the guide pipe (16) is connected with the inner cavity of the centrifugal machine (3), a control valve (17) is arranged on the guide pipe (16), the lower end of the right side wall of the centrifugal machine (3) is communicated with one end of a second liquid guide pipe (18), the other end of the second liquid guide pipe (18) is connected with the input end of a second infusion pump (19), and the output end of the second infusion pump (19) is connected with, the other end of third catheter (20) is connected with salting-out case (4), the last pipe wall of second catheter (18) is equipped with gets liquid mechanism, it includes shell body (21) to get liquid mechanism, the inside of shell body (21) just is in the last side wall of second catheter (18) is equipped with interior casing (22), the lower extreme of interior casing (22) with the inside of second catheter (18) is linked together, the lateral wall lower extreme of interior casing (22) is equipped with the round hole, the inside of interior casing (22) is equipped with rubber buffer (23) and the upper end of rubber buffer (23) is equipped with the one end of pull rod (24), the other end of pull rod (24) runs through interior casing (22) with the last side wall of shell body (21), shell body (21) with interior casing (22) are transparent material casing, the right side wall upper end of shell body (21) is equipped with the one end of collector tube (25) just the other end threaded connection of collector tube (25) has The liquid collecting bottle (26) is provided with scale marks on the side wall of the liquid collecting bottle (26), and the right end of the upper side wall of the salting-out box (4) is provided with a liquid injection port (27).

2. The apparatus for separating and purifying a bio-enzyme according to claim 1, wherein: and a pull ring (28) is arranged on the upper surface of the top cover (5).

3. The apparatus for separating and purifying a bio-enzyme according to claim 1, wherein: the upper end of the filter membrane tank (14) is in threaded connection with a membrane pressing cover (29), and a sanding block is arranged on the upper surface of the membrane pressing cover (29).

4. The apparatus for separating and purifying a bio-enzyme according to claim 1, wherein: and a diversion trench is arranged on the outer side of the liquid injection port (27).

5. The apparatus for separating and purifying a bio-enzyme according to claim 1, wherein: and the contact part of the liquid storage tank (2) and the top cover (5) is subjected to sealing treatment.