CN215668023U - Full-automatic adipose-derived stem cell extraction system - Google Patents

Abstract

The utility model relates to the technical field of medical instruments, in particular to a full-automatic adipose-derived stem cell extraction system which comprises a control device, a constant-pressure adipose extraction device, a multifunctional adipose treatment device and an automatic cell washing device, wherein the constant-pressure adipose extraction device, the multifunctional adipose treatment device and the automatic cell washing device are in signal connection with the control device. The full-automatic adipose-derived stem cell extraction system has high integration level, effectively reduces manual operation for extracting adipose-derived stem cells, and efficiently, accurately and automatically processes the whole process from adipose to the stem cells. Can improve the cell extraction efficiency and the biological safety; providing the most suitable condition for extracting the adipose-derived stem cells, and realizing high-efficiency integrated adipose-derived stem cell extraction; the negative pressure range is accurately regulated and controlled within the negative pressure range required by setting, the stability of the biological activity of the extracted fat particles is improved, the tissue damage of the liposuction part is reduced, and the safety of the operation is improved.

Description

Full-automatic adipose-derived stem cell extraction system

Technical Field

The utility model relates to the technical field of medical instruments, in particular to a full-automatic adipose-derived stem cell extraction system.

Background

At present, with the continuous development of stem cell application technology, the clinical demand for stem cell therapy is increasing. The adipose-derived stem cell is a mesenchymal stem cell extracted from adipose tissues, has the capacity of multi-directional differentiation, the capacity of synthesizing various growth factors and the capacity of promoting tissue regeneration and repair, and has proved to have good clinical effects on the treatment of various diseases. The adipose-derived stem cells have great application prospects, new requirements are provided for the method for separating the adipose-derived stem cells, and a faster and efficient separation method needs to be developed to separate the adipose-derived stem cells quickly and keep the high activity of the adipose-derived stem cells.

The obtaining of adipose-derived stem cells can be mainly divided into the following steps, firstly, adipose tissues are required to be obtained from a patient, and the liposuction operation is generally carried out; secondly, the obtained adipose tissues are processed and digested to obtain cell suspension; finally separating the required adipose-derived stem cells from the cell suspension. The whole process involves more steps and operations, is long in time consumption, and causes great obstruction to the clinical use of the adipose-derived stem cells.

The liposuction operation is that a doctor inserts a liposuction needle into a position to be liposucted, after a swelling solution is injected, subcutaneous fat is changed into fat particles and separated under the mechanical action through the reciprocating motion of the liposuction needle, and the separated fat particles are sucked out of a body through negative pressure suction so as to achieve the purposes of locally and rapidly slimming and obtaining fat tissues. The negative pressure is mainly provided by a vacuum pump and an injector for vacuum pumping. When a clinician performs liposuction, a proper negative pressure range is often needed, the traditional negative pressure range is between-45 KPa and-90 KPa, and the requirements of different parts on the negative pressure are slightly different. It has been shown that the negative pressure used during liposuction has an effect on both the activity and the number of extracted adipose stem cells. When the negative pressure is too large, tissues and blood vessels at the liposuction part are easily damaged, and the pumped adipose tissue cells are damaged to a certain extent, and when the negative pressure is too small, the fat pumping is not facilitated. When the liposuction operation is performed by using a vacuum pump, a doctor controls the air tightness through the air holes on the liposuction needle, or a pedal of the liposuction machine controls the vacuum pump to work, so that the negative pressure is adjusted. During the operation, doctors are required to frequently look away from the operation area, observe the negative pressure condition of the liposuction machine, and manually adjust the negative pressure to maintain the negative pressure in a coarse range which is not accurate. Therefore, not only the convenience of the surgical operation of the doctor but also the surgical safety of the patient are affected. When the injector is used for liposuction operation, the negative pressure generated by the injector is related to the volume specification and the drawing distance of the injector, the provided negative pressure is unstable, the negative pressure range can be estimated approximately only by the experience of a doctor, and the precision is poor.

Several systems for the automatic isolation of adipose-derived stem cells, which are currently commercially available on the market, include: PNC's Multi Station; CHA Biotech CHA-Station; cytori cell 800/CRS System; Medi-Khan's Lipokit with Max Stem. The existing automatic separation system is mainly designed based on an enzyme digestion method, extracted adipose tissues are digested and treated by collagenase, intercellular junctions are dissociated and fall off, adipose stem cells with high density and adipose cells with low density are layered in a centrifugal mode, and the adipose stem cells at the lower layer are collected. The main problems with this method are firstly the long time consuming, long time interaction with collagenase is required for adequate digestion of adipose tissue, and if one wants to improve the efficiency of cellular digestion, a higher concentration of collagenase is required, but a higher concentration of collagenase increases the residual ratio of collagenase, which is of animal or microbial origin, and increases the risk of harm of xenogeneic proteins to the human body after entering the human body. Even if the separation and extraction of adipose-derived stem cells by the traditional enzyme digestion method is carried out by a person with familiar technology, the separation can be completed within hours. Secondly, the digestion degree of collagenase is not easy to control, and whether appropriate digestion is achieved depends on the experience of operators, so that a longer learning curve is needed to achieve a better operation effect. If the digestion time is too short, insufficient digestion can be caused, and the cell yield is low; too long digestion time can lead to over digestion, and cell activity is reduced. In addition, the above systems have low level of intelligence and automation, and in the process of extracting stem cells, repeated manual operation is needed, and it may be necessary for the personnel involved in the operation to interrupt the operation or to arrange a special person to perform the operation of the system. In the process of repeated manual transfer, the problems of pollution and the like are easily caused.

SUMMERY OF THE UTILITY MODEL

In view of the above-mentioned shortcomings of the prior art, the present invention is directed to a fully automatic adipose stem cell extraction system, which solves the problems of the prior art.

In order to achieve the above objects and other related objects, the present invention provides a full-automatic adipose stem cell extraction system, which comprises a control device, and a constant pressure adipose extraction device, a multifunctional adipose processing device and an automatic cell washing device, which are in signal connection with the control device; the constant-pressure fat extraction device comprises a negative pressure unit and a fat extraction unit which are connected, wherein the negative pressure unit provides negative pressure for the fat extraction unit, the fat extraction unit comprises a liposuction needle and a fat collection bottle, and the liposuction needle is connected with the fat collection bottle through a catheter;

the multifunctional fat treatment device comprises a treatment unit and a power unit, wherein the treatment unit comprises a fat containing box and a heating element, the fat containing box is provided with an ultrasonic element, the heating element is used for heating the fat containing box, the power unit comprises a motor and a tray which are connected, the fat containing box is arranged on the tray, and the ultrasonic element, the heating element and the motor are all in signal connection with a control device.

The automatic cell washing device comprises a sample adding unit and a centrifugal unit, wherein the sample adding unit comprises a first conduit and a sample adding device, and the first conduit is connected with the sample adding device; the centrifugal unit includes the centrifuge rotor of V-arrangement centrifuging tube and adaptation V-arrangement centrifuging tube, the application of sample ware is arranged in to the application of sample in the V-arrangement centrifuging tube.

As described above, the fully automatic adipose-derived stem cell extraction system of the present invention has the following beneficial effects:

1) the high-integration fat stem cell extraction system effectively reduces the manual operation of extracting the fat stem cells, and the whole process from fat to the stem cells is efficiently, accurately and automatically processed.

2) Through ultrasonic treatment of fat, fat particles are reduced, the total contact area with collagenase is increased, the cell extraction efficiency is improved, the digestion time is shortened, the using amount of collagenase is reduced, and the biological safety of fat extraction is improved.

3) The multifunctional fat treatment workstation integrates equipment required by digestion of various cells, provides the most suitable condition for fat stem cell extraction, accurately detects fat content, improves the accuracy of collagenase dosage, and realizes efficient integrated fat stem cell extraction.

4) Through automatic liquid feeding, repeated washing and centrifugation in the V-shaped centrifuge tube, impurities in the cell suspension are effectively removed, the suspension required by the cells is obtained, and a plain tube is not required to be additionally arranged.

5) The constant-pressure fat extraction device controls the work of the vacuum pump through the processing terminal, realizes the accurate regulation and control of the negative pressure range in the negative pressure range required by setting, improves the stability of the biological activity of the extracted fat particles, reduces the tissue damage of the liposuction part, and improves the safety of the operation.

6) After a sufficient amount of fat is obtained in the liposuction process, the automatic fat processing process can be started, and the subsequent liposuction and the acquisition of adipose stem cells or adipose particles can be simultaneously carried out, so that the overall operation time from liposuction to fat filling or cell acquisition is shortened.

Drawings

FIG. 1 is a schematic view of a multifunctional fat processing device according to the present invention.

FIG. 2 is a schematic view of a multifunctional fat processing device according to the present invention.

FIG. 3 is a schematic view of an automatic cell washing apparatus according to the present invention.

FIG. 4 is a schematic view of an automatic cell washing apparatus according to the present invention.

FIG. 5 is a schematic diagram of the fully automatic adipose-derived stem cell extraction system of the present invention.

Description of the element reference numerals

1 display screen

2 processor

A1 negative pressure unit

A11 vacuum pump

A12 pedal switch

A13 barometer

A2 fat extraction unit

A21 liposuction needle

A22 fat collecting bottle

A3 safety bottle

B1 processing unit

B11 fat containing box

B111 liquid filling opening

B112 liquid outlet

B113 filter screen

B12 ultrasonic element

B13 heating element

B14 sensor

B141 temperature sensor

B142 pressure sensor

B2 power unit

B21 motor

B22 tray

C1 sample adding unit

C11 first conduit

C111 liquid inlet conduit

C112 cell entry catheter

C113 first valve

C12 sample injector

C121 power device

C122 sample adding head

C123 sample adding needle

C2 centrifugal unit

C21V-shaped centrifugal tube

Alpha inner included angle

C211 first opening

C212 second opening

Beta outer included angle

C2121 second valve

Scale of C213

C22 centrifuge rotor

C221 rotor barrel

C2211 second conduit

C2211a liquid conduit

C2211b cell catheter

C222 rotor cover

C2221 channel

Detailed Description

The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure.

Please refer to fig. 1 to 5. It should be understood that the structures, ratios, sizes, and the like shown in the drawings and described in the specification are only used for matching with the disclosure of the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions under which the present invention can be implemented, so that the present invention has no technical significance, and any structural modification, ratio relationship change, or size adjustment should still fall within the scope of the present invention without affecting the efficacy and the achievable purpose of the present invention. In addition, the terms "upper", "lower", "left", "right", "middle" and "one" used in the present specification are for clarity of description, and are not intended to limit the scope of the present invention, and the relative relationship between the terms and the terms is not to be construed as a scope of the present invention.

As shown in fig. 5, the present invention provides a full-automatic adipose-derived stem cell extraction system, which comprises a control device, and a constant pressure adipose extraction device, a multifunctional adipose processing device and an automatic cell washing device, which are in signal connection with the control device; the constant-pressure fat extraction device comprises a negative pressure unit A1 and a fat extraction unit A2 which are connected, wherein the negative pressure unit A1 provides negative pressure for the fat extraction unit A2, the fat extraction unit A2 comprises a fat extraction needle A21 and a fat collection bottle A22, and the fat extraction needle A21 is connected with the fat collection bottle A22 through a catheter;

the multifunctional fat treatment device comprises a treatment unit B1 and a power unit B2, wherein the treatment unit B1 comprises a fat containing box B11 and a heating element B13, the fat containing box B11 is provided with an ultrasonic element B12, the heating element B13 is used for heating the fat containing box B11, the power unit B2 comprises a motor B21 and a tray B22 which are connected, the fat containing box B11 is placed on the tray B22, and the ultrasonic element B12, the heating element B13 and the motor are in signal connection with a control device.

The automatic cell washing device comprises a sample adding unit C1 and a centrifugal unit C2, wherein the sample adding unit C1 comprises a first conduit C11 and a sample adding device C12, and the first conduit C11 is connected with a sample adding device C12; the centrifugal unit C2 comprises a centrifuge, a V-shaped centrifuge tube C21 and a centrifuge rotor C22 matched with the V-shaped centrifuge tube C21 are arranged in the centrifuge, and the sample adding device C12 is used for adding samples into the V-shaped centrifuge tube C21.

The control means is selected from the processor 2. The processor 2 may be implemented using a computer, integrated circuit module, programmable logic device, or other hardware as is known in the art.

The control device may be connected to the display screen 1. The display screen 1 may be a touch screen.

The negative pressure unit comprises a vacuum pump A11 and a pedal switch A12 connected. The pedal switch A12 is used to control the on or off of the vacuum pump A11.

The negative pressure unit also includes a barometer a13 for monitoring the pressure of the vacuum pump a 11. The barometer A13 and the vacuum pump A11 are both connected with the control device. The data of the barometer A13 is transmitted to the control device, and the control device controls the work of the vacuum pump, so that the negative pressure in the fat suction pipe is maintained in a set range, and the automatic regulation and the accurate regulation and the control of the negative pressure during the fat suction are realized.

In one embodiment, the bottom of the fat collection bottle a22 is provided with a plurality of outlets. One of the outlets may be connected to the multifunctional fat treatment device via a conduit. One of which is used to discharge waste liquid.

In one embodiment, the sidewall of the bottom of the fat collection bottle A22 is provided with a liquid level sensor for detecting the level change of the liquid.

In one embodiment, the constant pressure fat extraction device further comprises a safety bottle A3, wherein the safety bottle A3 is connected with the fat collection bottle a22 and the vacuum pump a11 through a conduit. The safety bottle A3 functions to prevent the fat collection bottle a22 from sucking too much liquid back into the vacuum pump a11 and damaging the machine.

The multifunctional fat processing device is provided with a fat containing box B11 for containing fat obtained by liposuction operation.

In one embodiment, the fat containment tank B11 is a sterile-processed container. For example, may be a disposable consumable for replaceable use. The disposable consumables can avoid cross contamination. Or may be a fixed container.

In one embodiment, the fat-containing tank B11 is provided with a filling opening B111. The filling port B111 may be provided with one or more, for example, two, three, or more. In one embodiment, the filling port B111 is provided with two ports, one for adding fat particles to the fat containing tank B11 and the other for adding enzymes.

In one embodiment, the filler opening B111 is provided with a sieve B113. The screen is not shown in the figure.

In one embodiment, the fat containing tank B11 is further provided with a liquid outlet B112. In one embodiment, the outlet B112 is provided with a screen B113. The strainer B113 is used to filter the treated fat digest solution, thereby removing fibrous tissue and a small amount of fat that is not completely digested. The liquid outlet B112 is connected with an automatic cell washing device. Specifically, the liquid outlet B112 is connected to a cell inlet conduit C112 of the automatic cell washing apparatus.

The multifunctional fat treatment device further comprises a plurality of pumps. The pump may be used to create negative or positive pressure in fat holding tank B11 to assist in the ingress or egress of fat digestive juices. The pump is for example a peristaltic pump. In one embodiment, the liquid inlet B111 or the liquid outlet B112 is externally connected to a liquid feeding pump or a liquid discharging pump, respectively. The pump functions to assist the flow of fat into the fat container B11 with negative pressure when the resistance is large as the fat flows from the charging port B111. When the outflow resistance of the fat digestive juice is large, positive pressure is provided to assist the outflow of the fat digestive juice. The pressure of the charge pump or the discharge pump can be supplied by a 11.

The ultrasonic element B12 is used for generating ultrasonic vibration into the fat containing box B11, on one hand, the fat in the fat containing box B11 is reduced in particle size under the mechanical action of the ultrasonic, and then after being filtered by the filter screen B113 with corresponding pore size, the micro fat particles which can be injected in a homogeneous way can be obtained, and the treatment of the fat before filling in the fat injection is completed. On the other hand, when the fat is digested by the ultrasonic treatment in combination with the enzyme such as collagenase added to the fat containing tank B11, the total contact area of the fat particles with collagenase is increased as the fat particles become smaller, the efficiency of collagenase digestion and fat extraction is improved, and the amount of collagenase used and the digestion time are reduced. Meanwhile, oil drops in fat cells can be separated out under the mechanical action of ultrasound, and extracellular matrix rich in interstitial cells can be obtained more efficiently.

The ultrasonic element B12 may be any element as long as it can transmit ultrasonic vibration into the fat-containing chamber B11. Preferably, the ultrasonic element B12 is inserted into the fat in the fat containing box B11 or attached to the outer wall of the fat containing box B11.

In one embodiment, the ultrasound element B12 is selected from an ultrasound probe.

In one embodiment, the heating element B13 is disposed within the tray B22 and is in signal communication with a control device. When the fat containing bin B11 is placed on the tray B22, the heating element B13 may heat the fat containing bin B11, and the control device controls the heating temperature.

In one embodiment, the heating element B13 is selected from a heating wire.

Further, the processing unit B1 also comprises a sensor B14, and the sensor B14 is in signal connection with a control device.

In one embodiment, the sensors B14 include a temperature sensor B141 and a pressure sensor B142.

The temperature sensor B141 detects the temperature of the fat containing tank B11 and transmits the detected temperature to the control device. The pressure sensor B142 is used for detecting the weight of the fat contained in the fat containing box B11 and transmitting the weight to the control device, the control device can further calculate the usage amount of the enzyme required by the fat treatment and feed the usage amount back to the display screen, and the collagenase solution with the corresponding volume is automatically added.

In one embodiment, the motor B21 is selected from linear motors. The motor provides power for tray B22 and makes tray B22 move, plays the effect of shaking table, and then drives fat and hold case B11 reciprocating motion, makes the fat granule and the liquid level in the case incessantly rock, fully contacts with the enzyme, makes the fat granule flow through ultrasonic probe repeatedly simultaneously, full play ultrasonic probe effect.

In one embodiment, the motor B21 is coupled to the tray B22 via bearings.

In one embodiment, the tray B22 is sized and shaped to hold a fat containing tank B11.

The operator can adjust the ultrasonic frequency and time, the shaking speed of the motor B21, the temperature, the digestion treatment time and other parameters through the display screen.

The first conduit C11 is used to add and transport cells or wash solution into the automatic cell washing apparatus. The first conduit C11 is provided with a first valve C113. In one embodiment, the first valve C113 is a three-way valve. Preferably, the switch is an electric control three-way switch. In one embodiment, the first valve C113 is also used to determine which conduit the liquid flows in. In one embodiment, the first valve C113 is a two-way switch or a three-way switch. The first valve C113 is an electric control switch or a manual switch. When the electric control switch is arranged, the automatic cell washing device is matched with the controller for use, and the controller is electrically connected with the first valve C113, so that the first valve C113 is controlled, and the aim of controlling liquid to flow in from different catheters is fulfilled.

The first conduit C11 is provided with one or more. In one embodiment, the first conduit C11 is provided with two, respectively, an inlet conduit C111 and a cell inlet conduit C112. The liquid inlet duct C111 is used for adding and conveying washing liquid, and the cell inlet duct C112 is used for adding and conveying cell suspension to be washed.

The sample applicator C12 includes a power device C121, a sample applicator C122, and a sample applicator C123, wherein the power device C121 is electrically connected to the sample applicator C122, and the sample applicator C123 is connected to the first conduit.

The pipeline above the sample adding head C122 is a soft conduit, and provides a space for the sample adding head C122 to move.

The sample adding head C122 can move up and down along the power device C121, and further drives the sample adding needle C123 to add liquid into the V-shaped centrifugal tube C21. The sample addition head C123 is used for fixing the connection of the first conduit C11 and the sample addition needle C123. In one embodiment, the power means C121 is selected from linear motors. The power unit C121 is fixed and the sample addition head C122 moves on its guide rail.

In one embodiment, the sample addition head C122 is a holder for the sample addition needle C123, so that the sample addition needle C123 can move up and down along the guide rail thereof along with the power device C121.

In one embodiment, the sample addition needle C123 is a hollow syringe-like needle.

The cross section of the V-shaped centrifugal tube C21 is V-shaped. The angle of the inner included angle α of the V-shape is not limited.

The V-shaped centrifuge tube C21 is provided with a first opening C211 at an internal included angle alpha. The first opening C211 may be used to add a sample to a V-shaped centrifuge tube C21.

A one-way channel is arranged at the first opening C211. In one embodiment, the one-way channel is a rubber stopper. The sample adding needle C123 moves downwards to puncture the rubber stopper and add the sample into the V-shaped centrifuge tube C21. The sample adding needle C123 withdraws the rubber plug when moving upwards, and the rubber plug is sealed again to prevent the liquid from flowing out.

The one-way passage may be replaced with a one-way valve. The application of sample needle C123 moves down, can carry out the application of sample operation behind the back-open check valve, when application of sample needle C123 moves upward and withdraws from, then the valve is closed, prevents that liquid from flowing out.

And a second opening C212 is formed in the outer included angle beta of the V-shaped centrifugal tube C21. The second opening C212 may be used to add or remove samples from the V-shaped centrifuge tube C21.

In one embodiment, a second valve C2121 is disposed at the second opening C212. The second valve C2121 is used for opening or closing the second opening C212. In one embodiment, the second valve C2121 is also used to determine which conduit the liquid flows from. In one embodiment, the second valve C2121 is a two-way switch or a three-way switch. The second valve C2121 is an electric control switch or a manual switch. When an electric control switch is arranged, the automatic cell washing device is matched with a controller, and the controller is electrically connected with the second valve C2121, so that the second valve C2121 is controlled, and the aim of controlling liquid to flow out from different catheters is fulfilled. When a manual switch is provided, the V-shaped centrifuge tube C21 can be removed from the centrifuge when liquid discharge is required, and the second valve C2121 is manually controlled to select the appropriate tube to discharge the liquid.

The two arms of the V-shaped centrifugal tube C21 are respectively cylindrical, and the tail part of the cylinder is conical or hemispherical.

And the wall of the V-shaped centrifugal tube C21 is provided with scales C213. The scale C213 is used to measure the volume of liquid in the centrifuge tube. The scale C213 can be set at different positions on the tube wall or set as a zero scale according to the specific use condition of the V-shaped centrifugal tube C21. For example, when the first opening C211 is used as an inlet of a centrifuge tube, since the liquid is first concentrated at the second opening C212 after entering, the zero scale can be set near the second opening C212; or when the second opening C212 is used as the inlet of the centrifuge tube, the liquid is firstly concentrated at the tail parts of the two arms of the centrifuge tube after entering, and the zero scale can be arranged at the tail part at the moment.

The centrifuge rotor C22 comprises a rotor barrel C221 and a rotor cover C222 which are matched, the rotor barrel C221 is provided with a V-shaped inner groove, the rotor cover C222 is provided with a conical part, and the V-shaped inner groove and the conical part are matched with the shape of a V-shaped centrifuge tube C21. The bottom of the V-shaped inner groove is provided with a concave part. The recess is used for placing the second valve C2121.

In one embodiment, a channel C2221 is provided on the central shaft of the rotor cover C222. The central shaft is as follows: in the working state, the vertical line made from the vertex of the conical part to the horizontal plane is crossed. The channel C2221 is used for the sample adding needle C123 to pass through and add the sample to the V-shaped centrifugal tube C21.

A second conduit C2211 is arranged inside the rotor barrel C221, and an opening at one end of the second conduit C2211 is arranged at the bottom of the V-shaped inner groove. The V-shaped centrifugal tube C21 is connected with the three-way valve and then embedded into the V-shaped inner groove of the rotor barrel C221, and the three-way valve is just embedded into the electric control valve C2121. The second conduit C2211 is provided with one or more. When a second conduit C2211 is provided, cells and fluid enter or exit the same conduit. In one embodiment, the second conduit C2211 is provided with two, respectively a liquid conduit C2211a and a cell conduit C2211 b.

A recovery bottle is arranged at the tail end of the second conduit C2211. A common reagent bottle may be used as the recovery bottle. The recovery bottle comprises a cell recovery bottle and a waste liquid recovery bottle.

And a rotor rotating shaft is arranged at the bottom of the rotor barrel C221. In one embodiment, the rotor shaft is hollow.

The full-automatic adipose-derived stem cell extraction system further comprises a centrifuge, and the centrifuge is in signal connection with the processor. The automatic cell washing device needs to be matched with a centrifuge for use, and the type and the model of the centrifuge are not particularly limited. The automatic cell washing device may also be used in conjunction with a fat treatment device.

The automatic cell washing device can be used to wash various cells, such as adipocytes.

The use method of the full-automatic adipose-derived stem cell extraction system comprises the following steps:

before liposuction, all the components in the system are installed and debugged, for example, a sterile fat containing box B11 is installed on a tray B22, a two-way or three-way valve is embedded into a corresponding electric control switch and connected with a corresponding conduit, parameters such as ultrasonic frequency, ultrasonic time, shaking speed, temperature, digestion treatment time and the like of the multifunctional fat treatment device processor are set (for example, the ultrasonic frequency is 50-200W, the ultrasonic time is 15-150 seconds, the shaking speed is 80-150 rpm, the temperature is 37 ℃ and the digestion treatment time is 20-30 minutes), a V-shaped centrifuge tube C21 is installed on a centrifuge rotor C22, the centrifuge rotor C22 is installed in a centrifuge, a sample adding needle C123 is fixed on a sample adding head C122, and a cell inlet conduit C112 is connected with a liquid outlet B112 of the multifunctional fat treatment device.

Taking the range of the negative pressure of liposuction from-45 KPa to-90 KPa as an example, setting a required range on a touch screen, puncturing a liposuction needle into a position needing liposuction, stepping down a pedal switch to control a vacuum pump to start working, measuring the air pressure in a pipeline by an air pressure meter, feeding back the air pressure value through the touch screen, controlling the vacuum pump to provide small suction force or stop working by a processor when the air pressure reaches-90 KPa, keeping the air pressure relatively stable, operating the liposuction needle by a doctor to start liposuction, and controlling the vacuum pump to recover working by the processor when the negative pressure is gradually reduced in the operation and reaches-45 KPa so as to keep the negative pressure in the process of liposuction at-45 KPa to-90 KPa. When the liposuction is finished or needs to be suspended, the pedal switch is stepped down, and the vacuum pump is turned off. The mixture of extracted fat and tumescent fluid passes through the catheter to the fat collection bottle. The mixed liquid of fat and swelling liquid is layered gradually after a period of time that stews in the fat receiving flask, for the swelling liquid layer of upper fat layer and lower floor, the level sensor of fat receiving flask bottom lateral wall detects the liquid level change (the mechanism is optics generally), and controlling means opens the export that is used for discharging the waste liquid of fat receiving flask bottom, discharge swelling liquid. The fat layer descends gradually with swelling liquid boundary liquid level, and after swelling liquid thoroughly flowed out, interface reached level sensor, and level sensor sensed the change, fed back to controlling means, and controlling means control outlet is closed, prevents that the fat layer from flowing out. In addition, after a sufficient amount of fat is obtained during the liposuction procedure, a fat collection bottle may be added for collection of new fat, and the original fat collection bottle may start processing fat, so that the subsequent liposuction procedure and the acquisition of adipose stem cells or adipose particles may be simultaneously performed.

Fat particles in the fat collecting bottle are added into the fat containing box B11 through the liquid adding opening B111, when the resistance of the fat flowing into the fat containing box B11 is large, a power switch of the liquid adding pump is turned on, the fat particles are filtered through the filter screen B113 with the required aperture under the assistance of the negative pressure of the liquid adding pump, and the fat particles with the corresponding particle size are collected. The pressure sensor B142 detects the weight of the fat in the fat containing tank B11, calculates the required collagenase content according to a preset formula, and displays the collagenase content on the touch screen. The doctor or nurse prepares a corresponding volume and concentration of collagenase solution, injects the prepared collagenase solution into the fat-containing tank B11, or automatically adds a corresponding volume of collagenase solution under the control of the processor. The multifunctional fat treatment device treats fat according to preset parameters, the heating wire starts to heat, the temperature sensor 141 detects the temperature in the fat containing box 11 and feeds the temperature back to the processor, and the heating wire is heated to a set temperature and maintained. The fat containing box 11 reciprocates according to a set frequency under the action of the linear motor, fat shakes therein, and the fat is separated into small particles under the mechanical action of the ultrasonic probe. Meanwhile, under the action of collagenase, cells in the adipose tissue are gradually separated and flow out through the liquid outlet 112, and when the fat digestion liquid flows out from the outflow port with large resistance, the liquid outlet pump forms positive pressure in the fat containing box 11 to assist the fat digestion liquid to flow out. Then the next step of adipose-derived stem cell centrifugation can be carried out.

The linear motor drives the sample adding head C122 to further drive the sample adding needle C123 to move downwards, the sample adding needle C123 penetrates through the one-way valve, the first valve C113 selects the channel C112, the cell suspension enters the cell entering conduit C112 to add the cells into the V-shaped centrifuge tube C21, the liquid volume can be observed through the scales on the tube wall of the V-shaped centrifuge tube C21, the liquid volume is in the most suitable processing range, and the liquid level is between the highest scale and the lowest scale of the V-shaped centrifuge tube C21. The pressure sensor can estimate the total fat digestive juice volume through the total pressure, and the centrifugation is carried out according to the volume distribution of the V-shaped centrifugal tube C21 in batches, and meanwhile, according to the change value of the pressure, the volume of the added fat digestive juice is sensed, and the liquid with the corresponding volume is automatically added. If the V-shaped centrifuge tube C21 is centrifuged by 100ml at most, and the pressure sensor measures that 200ml of fat digestion liquid exists in the fat holding tank, the centrifugation washing is intelligently selected to be carried out in 2 batches. After the cell suspension enters the conduit C112 and the cells are added into the V-shaped centrifuge tube C21, the pressure sensor detects the pressure change, and the liquid adding is automatically stopped after the pressure is reduced to 100 ml. The sampling needle C123 is pulled out, the one-way valve is closed, and the liquid is prevented from flowing out. Setting the centrifugal time and centrifugal force on a centrifuge or other control equipment, starting centrifugation according to the preset centrifugal time and centrifugal force, enabling the cell suspension to flow to two arms of the V-shaped centrifugal tube C21 under the action of the centrifugal force, and enabling the liquid volumes of the two arms to be uniformly distributed. After centrifugation, rotation is stopped slowly, liquid is slowly converged to the second opening C212 again under the action of gravity, cells are adhered to the tail parts of the two arms of the V-shaped centrifugal tube C21, the second valve C2121 is opened to discharge waste liquid, the waste liquid flows out along the second conduit C2211 and is collected by a waste liquid collecting bottle to prevent liquid from splashing, and the second valve C2121 is closed after the waste liquid is discharged. The first valve C113 is opened, the channel C111 is selected, the liquid adding guide pipe is controlled by the processor to automatically and rapidly add PBS buffer solution with corresponding volume for washing cells, the first valve C113 is automatically closed after the liquid level of the added liquid reaches a proper water level line, the linear motor is lifted again, the liquid adding needle C123 is withdrawn, the rotor rapidly rotates to drive the V-shaped centrifugal tube C21, adhered cells are washed off, the cells are prevented from being dried, and the cells and the PBS are mixed for re-suspension. After resuspension, centrifugation is performed again, the cells are attached to the top of the two arms of the V-shaped centrifuge tube C21 again, the second valve C2121 is opened, and the waste fluid is discharged from the second conduit C2211 and then the second valve C2121 is closed. Repeating the above washing process for 2-3 times. And finally, in washing, after PBS is added, the rotor is quickly started to rotate for 2-3 times to drive the V-shaped centrifuge tube C21 to rotate, the PBS quickly washes the cells adhered to the top of the V-shaped centrifuge tube C21 from the bottom to the top of the V-shaped centrifuge tube C21 to prevent the cells from being dried, and the cells and the PBS are mixed and resuspended. The cell-containing PBS cell suspension was allowed to re-pool under gravity to the second opening C212. The second valve C2121 is opened to discharge the cells from the second conduit C2211. Alternatively, after the last resuspension of the cells, the V-tube C21 is removed from the centrifuge rotor C22 and the cell suspension is collected by manually opening the second valve C2121.

In conclusion, the present invention effectively overcomes various disadvantages of the prior art and has high industrial utilization value.

The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the utility model. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims (11)

1. A full-automatic adipose-derived stem cell extraction system is characterized by comprising:

1) constant pressure fat extraction device: the constant-pressure fat extraction device comprises a negative pressure unit (A1) and a fat extraction unit (A2) which are connected, wherein the negative pressure unit (A1) provides negative pressure for the fat extraction unit (A2), the fat extraction unit (A2) comprises a fat extraction needle (A21) and a fat collection bottle (A22), and the fat extraction needle (A21) is connected with the fat collection bottle (A22) through a catheter;

2) multifunctional fat treatment device: the multifunctional fat treatment device comprises a treatment unit (B1), a power unit (B2), wherein the treatment unit (B1) comprises a fat containing box (B11) and a heating element (B13), the fat containing box (B11) is provided with an ultrasonic element (B12), the heating element (B13) is used for heating the fat containing box (B11), the power unit (B2) comprises a motor (B21) and a tray (B22) which are connected, the fat containing box (B11) is placed on the tray (B22), and the fat containing box (B11) is connected with a fat collecting bottle (A22);

3) automatic cell washing device: the automatic cell washing device comprises a sample adding unit (C1) and a centrifugal unit (C2), wherein the sample adding unit (C1) comprises a first conduit (C11) and a sample adding device (C12), one end of the first conduit (C11) is connected with the fat containing box (B11), and the other end of the first conduit is connected with the sample adding device (C12); the centrifugal unit (C2) comprises a centrifuge, a V-shaped centrifuge tube (C21) and a centrifuge rotor (C22) matched with the V-shaped centrifuge tube (C21) are arranged in the centrifuge, and the sample applicator (C12) is used for applying samples to the V-shaped centrifuge tube (C21);

4) a control device: the control device is in signal connection with the constant-pressure fat extraction device, the multifunctional fat processing device and the automatic cell washing device.

2. The fully automatic adipose stem cell extraction system according to claim 1, wherein the negative pressure unit comprises a vacuum pump (A11) and a pedal switch (A12) connected, and a barometer (A13) for monitoring the pressure of the vacuum pump (A11), wherein the barometer (A13) and the vacuum pump (A11) are connected with a control device.

3. The fully automatic adipose stem cell extraction system of claim 1, wherein the bottom of the adipose collection bottle (A22) is provided with a plurality of outlets, one of which is connected with the adipose containing box (B11).

4. The fully automatic adipose-derived stem cell extraction system of claim 1, wherein the fat containing tank (B11) is provided with a liquid inlet (B111) and/or a liquid outlet (B112).

5. The fully automatic adipose-derived stem cell extraction system of claim 1, wherein the ultrasound element (B12) is inserted into the inner cavity of the adipose-derived containment tank (B11) or attached to the outer wall of the adipose-derived containment tank (B11); and/or the heating element (B13) is placed inside the tray (B22).

6. The fully automatic adipose stem cell extraction system according to claim 1, wherein the processing unit (B1) further comprises a sensor (B14), the sensor (B14) being in signal connection with a control device.

7. The fully automatic adipose-derived stem cell extraction system of claim 1, wherein the sample applicator (C12) comprises a power device (C121), a sample applicator head (C122), and a sample applicator needle (C123), wherein the power device (C121) is electrically connected to the sample applicator head (C122), and the sample applicator head (C122) is connected to the first conduit (C11) and the sample applicator needle (C123).

8. The fully automated adipose stem cell extraction system of claim 1, wherein the V-shaped centrifuge tube (C21) further comprises one or more of the following features:

1) a first opening (C211) is arranged at the inner included angle (alpha);

2) a second opening (C212) is arranged at the outer included angle (beta);

3) the pipe wall is provided with scales (C213).

9. The fully automatic adipose-derived stem cell extraction system of claim 8, wherein a one-way valve is provided at the first opening (C211); or a second valve (2121) is arranged at the second opening (C212).

10. The fully automated adipose stem cell extraction system of claim 1, wherein the centrifuge rotor (C22) comprises a rotor barrel (C221) and a rotor cover (C222) that are adapted, the rotor barrel (C221) is provided with a V-shaped inner groove, the rotor cover (C222) is provided with a tapered portion, and the V-shaped inner groove and the tapered portion are matched with the shape of a V-shaped centrifuge tube (C21).

11. The fully automated adipose stem cell extraction system of claim 1, wherein the control device is selected from a processor.

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