CN219567918U - Adenovirus vector purification device - Google Patents

Abstract

The utility model discloses an adenovirus vector purification device which comprises a device shell, wherein the upper end of the device shell is fixedly connected with a fixing frame, the inner wall of the device shell is provided with a groove, the lower side of the outer wall of the device shell is in threaded connection with a protective cover, the lower end of the fixing frame is fixedly connected with a motor, the output shaft of the motor is fixedly connected with a centrifugal barrel, the upper end of the centrifugal barrel is provided with an inlet, the periphery of the centrifugal barrel is provided with centrifugal holes, the outer wall of the centrifugal barrel is fixedly connected with a limiting block, and the inner wall of the protective cover is provided with an ultrafiltration template. According to the utility model, through the centrifugal barrel, the centrifugal barrel is driven by the motor to rotate, impurities of adenovirus in the centrifugal barrel are discharged through the centrifugal holes, and the impurities slide onto the ultrafiltration template through the inner wall of the device shell under the action of gravity after being discharged, so that the problem that the traditional device needs to convey the adenovirus to the ultrafiltration template in a complex mode after centrifuging the adenovirus is solved.

Description

Adenovirus vector purification device

Technical Field

The utility model relates to the technical field of purification, in particular to an adenovirus vector purification device.

Background

When adenovirus is used for in vivo experiments, adenovirus needs to be purified, and the currently common methods include a supermembrane filtration method, a centrifugation method and the like, and the adenovirus purification often needs a large number of virus cells so as to facilitate chemical research and preparation of corresponding antibodies; however, the existing adenovirus purification device still has the defects:

for example, the adenovirus vector purification device with the publication number of CN217351344U solves the problem of facilitating the purification of adenovirus vectors subjected to centrifugal layering, thereby facilitating the discharge of the purified adenovirus vectors from a main valve tube and improving the convenience of adenovirus vector purification;

the device is additionally provided with the ultrafiltration templates for double purification on the basis of centrifugal purification, but is additionally provided with a plurality of pipelines for conveying the adenovirus vectors subjected to centrifugal layering to the ultrafiltration templates, so that the practical mode is more troublesome, the ultrafiltration templates are inconvenient to take out for cleaning or replacing after being used, the ultrafiltration templates are easy to pollute, and impurity liquid after the adenovirus is purified needs to be collected and is required to be discharged into sterilizing water for sterilization, and the adenovirus can not be discharged at will, so that the adenovirus is easy to spread.

Disclosure of Invention

(one) solving the technical problems

Aiming at the defects of the prior art, the utility model aims to provide an adenovirus vector purification device which solves the problems that the centrifuged liquid is conveyed to a filter component in a complex mode, the filter component is inconvenient to detach and replace or clean, and impurities after adenovirus purification are inconvenient to collect and disinfect.

(II) technical scheme

In order to achieve the above object, the present utility model provides the following technical solutions, in which an adenovirus vector purification apparatus is adopted: including the device shell, the upper end fixedly connected with mount of device shell, the inner wall of device shell is provided with the recess, and the downside threaded connection of the outer wall of device shell has the protective cover, the lower extreme fixedly connected with motor of mount, fixedly connected with centrifugation bucket on the output shaft of motor, the upper end of centrifugation bucket is provided with the entry, and is provided with the centrifugation hole around the centrifugation bucket to the outer wall fixedly connected with stopper of centrifugation bucket, the ultrafiltration template is installed to the inner wall of protective cover.

As an optimal scheme, the center line of the centrifugal barrel and the center line of the device shell are on the same straight line, and the centrifugal holes are distributed on the centrifugal barrel at equal intervals.

As the preferred scheme, the stopper is rotationally connected with the groove, and the cross section of stopper is semi-circular, and the stopper symmetric distribution is in the left and right sides of centrifugal barrel.

As the preferred scheme, the inner wall fixedly connected with supporting shoe of shield lid is provided with first spout in the supporting shoe, and is connected with fixed frame on the supporting shoe, the bottom fixedly connected with first spring in the first spout, the upper end fixedly connected with first slider of first spring, first slider and first spout are sliding connection, fixed frame and ultrafiltration template are fixed connection.

As the preferred scheme, the inner wall of shield cap is provided with the second spout, and the inside left side fixedly connected with second spring of second spout, the right-hand member fixedly connected with second slider of second spring, second slider and second spout are sliding connection.

Preferably, the first sliding block and the first sliding groove form a sliding structure through a first spring, and one side of the second sliding block is inclined.

(III) beneficial effects

Compared with the prior art, the utility model provides an adenovirus vector purification device, which comprises the following components

The beneficial effects are that:

1. through the centrifugation bucket that sets up, drive the centrifugation bucket through the motor and rotate, discharge the impurity of adenovirus in the centrifugation bucket through the centrifugation hole, make impurity through the inner wall of device shell slide to the ultrafiltration template on under the effect of gravity after the discharge, solved current device and need carry the problem to the ultrafiltration template through complicated mode after to adenovirus centrifugation:

2. the device is provided with the protective cover, after adenovirus is subjected to secondary purification through the ultrafiltration template, impurity liquid flows into the bottom end of the protective cover, and the impurity purified by adenovirus is conveniently disinfected by pouring disinfection water into the bottom end of the protective cover, so that the problem that the impurity purified by adenovirus in the existing device is inconvenient to collect and disinfect is solved;

3. the device is provided with first slider, slides the second slider to the second spout in for the second slider loses the connection with the fixed frame, drives first slider upwards slide under the effect of first spring this moment, thereby upwards lift the fixed frame, has solved the inconvenient ultrafiltration template dismantlement after the purification adenovirus of current device and has washd or change the problem.

Drawings

FIG. 1 is a schematic view of the overall structure of the present utility model;

FIG. 2 is a schematic elevational cross-sectional view of the structure of the present utility model;

FIG. 3 is a schematic diagram of the connection structure of the centrifugal barrel and the limiting block;

fig. 4 is an enlarged schematic view of the structure a in fig. 2 according to the present utility model.

In the figure: 1. a device housing; 2. a fixing frame; 3. a motor; 4. a centrifugal barrel; 5. an inlet; 6. centrifuging the hole; 7. a groove; 8. a limiting block; 9. a protective cover; 10. a support block; 11. a first chute; 12. a first spring; 13. a first slider; 14. a fixed frame; 15. ultrafiltering the template; 16. a second chute; 17. a second spring; 18. and a second slider.

Detailed Description

The utility model is further illustrated and described below in conjunction with the specific embodiments and the accompanying drawings:

referring to fig. 1-4, the present utility model: including device shell 1, device shell 1's upper end fixedly connected with mount 2, device shell 1's inner wall is provided with recess 7, device shell 1's downside threaded connection of outer wall has shield cover 9, the lower extreme fixedly connected with motor 3 of mount 2, fixedly connected with centrifugation bucket 4 on motor 3's the output shaft, centrifugation bucket 4's upper end is provided with entry 5, and centrifugation bucket 4 is provided with centrifugal hole 6 all around, and centrifugation bucket 4's outer wall fixedly connected with stopper 8, shield cover 9's inner wall installs ultrafiltration template 15, make adenovirus after the centrifugation can directly fall on ultrafiltration template 15, make purification through ultrafiltration template 15 that can be quick after the centrifugation layering.

The central line of the centrifugal barrel 4 and the central line of the device shell 1 are on the same straight line, and the centrifugal holes 6 are distributed on the centrifugal barrel 4 at equal intervals, so that the centrifugal barrel 4 can stably rotate on the same central line, and the centrifugal separation process is ensured to be more stable.

The limiting block 8 is rotationally connected with the groove 7, the cross section of the limiting block 8 is semicircular, the limiting blocks 8 are symmetrically distributed on the left side and the right side of the centrifugal barrel 4, and the limiting block 8 and the groove 7 rotate to limit and rotate the centrifugal barrel 4, so that the centrifugal barrel 4 is guaranteed; stability of the centrifuging process of the centrifuge bowl 4.

The inner wall fixedly connected with supporting shoe 10 of protective cover 9 is provided with first spout 11 in the supporting shoe 10, and is connected with fixed frame 14 on the supporting shoe 10, the bottom fixedly connected with first spring 12 in the first spout 11, the first slider 13 of upper end fixedly connected with of first spring 12, first slider 13 is sliding connection with first spout 11, fixed frame 14 is fixed connection with ultrafiltration template 15, be convenient for change and wash ultrafiltration template 15, prevent that ultrafiltration template 15 from using in succession from causing experimental pollution, lead to experimental result to appear unusual.

The inner wall of protective cover 9 is provided with second spout 16, and the inside left side fixedly connected with second spring 17 of second spout 16, the right-hand member fixedly connected with second slider 18 of second spring 17, second slider 18 and second spout 16 are sliding connection, can carry out the block to filter assembly through second slider 18, guarantee filter assembly's stability when using.

The first slider 13 forms a sliding structure with the first chute 11 through the first spring 12, and one side of the second slider 18 is inclined, and the second slider 18 can slide through pressing through the arrangement of the inclined shape, and can be sprung up through the sliding structure, so that the filter assembly can be disassembled quickly.

The working principle of the utility model is that; referring to fig. 1-3, firstly pouring sterilizing water into the bottom of a protective cover 9, pouring adenovirus into the protective cover from an inlet 5, then starting a motor 3, driving a centrifugal barrel 4 to rotate by the motor 3, enabling a limiting block 8 to rotate in a groove 7, layering adenovirus through a centrifugal hole 6, enabling the layered adenovirus to fall on an ultrafiltration template 15 in the protective cover 9 along the inner wall of a device shell 1, and filtering the layered adenovirus by the ultrafiltration template 15, so that impurity liquid falls on the bottom of the protective cover 9, and sterilizing impurities by the sterilizing water;

as shown in fig. 2 and 4, the protecting cover 9 is separated from the apparatus housing 1 by rotating the protecting cover 9, and then the second slider 18 is slid along the inside of the second chute 16, at this time the first slider 13 is slid upward under the action of the first spring 12, and the first slider 13 is slid upward while supporting the fixing frame 14 upward, so that the ultrafiltration template 15 on the fixing frame 14 is conveniently and rapidly detached, and then a new fixing frame 14 and ultrafiltration template 15 are placed on the supporting block 10, and by pressing the fixing frame 14, the second slider 18 is slid along the inside of the second chute 16 by the inclined shape of the second slider 18, so that the fixing frame 14 pushes the first slider 13 downward, and then the second slider 18 is slid outward under the action of the second spring 17, so that the ultrafiltration template 15 on the fixing frame 14 is rapidly installed.

Finally, it should be noted that the above embodiments are only for illustrating the technical solution of the present utility model, and not for limiting the scope of the present utility model, and although the present utility model has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made to the technical solution of the present utility model without departing from the spirit and scope of the technical solution of the present utility model.

Claims (6)

1. An adenovirus vector purification apparatus comprising an apparatus housing (1), characterized in that: the utility model discloses a centrifugal device for the medical equipment, including device shell (1), fixed frame (2) is fixed to the upper end of device shell (1), the inner wall of device shell (1) is provided with recess (7), the downside threaded connection of the outer wall of device shell (1) has protecting cover (9), the lower extreme fixedly connected with motor (3) of fixed frame (2), fixedly connected with centrifugation bucket (4) on the output shaft of motor (3), the upper end of centrifugation bucket (4) is provided with entry (5), and be provided with centrifugal hole (6) around centrifugation bucket (4) to outer wall fixedly connected with stopper (8) of centrifugation bucket (4), ultrafiltration template (15) are installed to the inner wall of protecting cover (9).

2. An adenovirus vector purification apparatus as claimed in claim 1, wherein: the center line of the centrifugal barrel (4) and the center line of the device shell (1) are on the same straight line, and the centrifugal holes (6) are distributed on the centrifugal barrel (4) at equal intervals.

3. An adenovirus vector purification apparatus as claimed in claim 1, wherein: the limiting blocks (8) are rotationally connected with the grooves (7), the cross sections of the limiting blocks (8) are semicircular, and the limiting blocks (8) are symmetrically distributed on the left side and the right side of the centrifugal barrel (4).

4. An adenovirus vector purification apparatus as claimed in claim 1, wherein: the inner wall fixedly connected with supporting shoe (10) of shield cap (9), be provided with first spout (11) in supporting shoe (10), and be connected with fixed frame (14) on supporting shoe (10), the bottom fixedly connected with first spring (12) in first spout (11), the upper end fixedly connected with first slider (13) of first spring (12), first slider (13) are sliding connection with first spout (11), fixed frame (14) are fixed connection with ultrafiltration template (15).

5. The adenovirus vector purification apparatus of claim 4, wherein: the inner wall of the protective cover (9) is provided with a second sliding groove (16), a second spring (17) is fixedly connected to the left side inside the second sliding groove (16), a second sliding block (18) is fixedly connected to the right end of the second spring (17), and the second sliding block (18) is in sliding connection with the second sliding groove (16).

6. An adenovirus vector purification apparatus as claimed in claim 5, wherein: the first sliding block (13) and the first sliding groove (11) form a sliding structure through the first spring (12), and one side of the second sliding block (18) is inclined.