CN112080406A - Stem cell separation device - Google Patents
Stem cell separation device Download PDFInfo
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- CN112080406A CN112080406A CN202011013380.4A CN202011013380A CN112080406A CN 112080406 A CN112080406 A CN 112080406A CN 202011013380 A CN202011013380 A CN 202011013380A CN 112080406 A CN112080406 A CN 112080406A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
Abstract
The invention discloses a stem cell separation device, which relates to the technical field of cell separation and comprises a base, a supporting table, a centrifugal mechanism, a heating mechanism, a filtering mechanism, a cleaning mechanism and a stem cell culture box, wherein the base is arranged on the ground, the supporting table is fixedly arranged on the base, the centrifugal mechanism is fixedly arranged on the supporting table, the heating mechanism is fixedly arranged in the centrifugal mechanism, the filtering mechanism is arranged on one side of the centrifugal mechanism 3, the cleaning mechanism is arranged on the other side of the centrifugal mechanism, and the stem cell culture box is fixedly arranged on the base Grow, reproduce and maintain the main structure and function.
Description
Technical Field
The invention relates to the technical field of cell separation, in particular to a stem cell separation device.
Background
A stem cell is a cell that has self-renewal capacity as well as the potential for multi-directional differentiation. Stem cells can be divided into three major classes according to differentiation potential: totipotent, pluripotent, and unipotent stem cells. According to the developmental stage, stem cells can be further classified into: embryonic stem cells and adult stem cells. Due to ethical limitations, most of the stem cells currently used in clinical research and applications are adult stem cells. Adult stem cells are derived from many tissues and organs of adult animals, including hematopoietic stem cells, neural stem cells, adipose stem cells, bone marrow mesenchymal stem cells, umbilical cord blood stem cells, and the like.
At present, most of separation methods of stem cells are collagenase digestion methods, so that the cell yield is low, the digestion time is not easy to control, and the activity of the cells is easy to influence, so that the stem cells have a great bottleneck in clinical application, while the separation method which is frequently used is a centrifugal separation method, but the stem cells are seriously crushed by high-speed rotation in the centrifugal process, the blood components cannot be separated without damage, and the cell activity in the centrifugal process is poor, so that the separation is too slow, and the separation effect is poor.
Disclosure of Invention
The present invention provides a stem cell separation device to solve the above technical problems in the prior art.
The utility model provides a stem cell separation device, includes base, brace table, centrifugal mechanism, heating mechanism, filter equipment, wiper mechanism and stem cell incubator, the base sets up subaerial, the fixed setting on the base of brace table, centrifugal mechanism is fixed to be set up on the brace table, heating mechanism is fixed to be set up in the centrifugal mechanism, filter equipment sets up one side of centrifugal mechanism, the wiper mechanism sets up the opposite side at centrifugal mechanism, the stem cell incubator is fixed to be set up on the base.
Further, the centrifugal mechanism comprises a centrifugal assembly and a centrifugal box, the centrifugal box is fixedly arranged on the supporting table, and the centrifugal mechanism is arranged inside the centrifugal box.
Further, the centrifuge box includes feed inlet, discharge gate and box, the feed inlet is fixed to be set up on the box top, the one end of discharge gate is connected with the bottom of box, the other end of discharge gate is connected with filtering mechanism's water delivery end.
Further, the centrifugal assembly comprises a fixed plate, two installation seats, two first motors, two first belt wheels, two belts, two second belt wheels, two second motors, two first bevel gears, two second bevel gears, a screw rod, a rotating rod, a push rod, two rotating plates, two stirring rods, two stirring blades, two fixing frames and two mounting frames, wherein the fixed plate is fixedly arranged on the top wall in the centrifugal box, the two installation seats are respectively and fixedly arranged on two sides of the fixed plate, the first motors are fixedly arranged on one sides of the installation seats, the two fixing frames are respectively and fixedly arranged on the inner sides of the two installation seats, the first belt wheels are connected with the output ends of the first motors, the second motors are fixedly arranged on the other sides of the installation seats, the output ends of the second motors penetrate through the installation seats and the fixing frames, and the output ends of the second motors extend to the other sides of the fixing frames, the utility model discloses a motor, including first bevel gear, second motor, belt pulley, belt sleeve, push rod, first bevel gear, mounting bracket, second bevel gear, second belt pulley, conveyer belt, pulley.
Furthermore, heating mechanism includes control panel, heating ring and heating pipe, control panel sets up in the outside of centrifugal box, the heating pipe is equipped with a plurality of, a plurality of the heating pipe evenly sets up on the heating ring, just the input setting of heating pipe is on the heating ring, the heating ring sets up in the inboard of centrifugal box, a plurality of the heating pipe is all pegged graft in the tank wall of centrifugal box.
Furthermore, filtering mechanism includes input tube, first suction pump, rose box, first filter membrane layer, second filter membrane layer and wastewater disposal basin, the input of first suction pump with the discharge gate is connected, the one end of input tube is connected with the output of first suction pump, the rose box is fixed to be set up on the base, the other end and the rose box of input tube are connected, first filter membrane layer sets up in the rose box, the second filter membrane layer sets up the downside at first filter membrane layer, just the filtration pore radius of first filter membrane layer is less than the filtration pore radius of second filter membrane layer, just one side of first filter membrane layer and second filter membrane layer all is equipped with the handle, the filtration pore diameter of first filter membrane layer and second filter membrane layer is-nm, the wastewater disposal basin sets up the bottom of rose box, just one side of wastewater disposal basin is equipped with the handle.
Furthermore, the cleaning mechanism comprises a water tank, a water inlet, a second water suction pump, a first water delivery pipe, a second water delivery pipe and spray heads, the water tank is arranged on the base and is arranged on one side of the centrifugal mechanism, the water inlet is arranged at the top of the water tank, the first water delivery pipe is arranged in the water tank, one end of the first water delivery pipe penetrates through the water tank and is connected with the input end of the second water suction pump, the output end of the second water suction pump is connected with the second water delivery pipe, the second water delivery pipe is arranged on the upper side of the centrifugal mechanism, the spray heads are arranged in two, the spray heads are arranged on the second water delivery pipe, and the spray heads penetrate through the centrifugal box and extend to the inner side of the centrifugal box.
Further, the stem cell incubator is the prior art, and the stem cell incubator is arranged on one side of the filtering mechanism.
Compared with the prior art, the invention has the beneficial effects that:
firstly, the stem cells are separated by the centrifugal mechanism, the activity of the stem cells in the separation process is ensured by the heating mechanism, the stem cells are filtered and extracted by the filtering mechanism, the cleanliness of the centrifugal mechanism is ensured by the cleaning mechanism, the separation operation is not influenced by impurities, the stem cells are enabled to survive, grow and reproduce and maintain the main structure and functions by the stem cell incubator, meanwhile, the structure is simple, the operation is convenient, the working efficiency is greatly improved, and manpower and material resources are saved.
Secondly, the centrifugal mechanism is arranged to generate rapid and repeated flexible extrusion and crushing, so that hard tissues are prevented from scattering and flying to take away soft tissues, the integrity of cells is guaranteed, and the cells are prevented from being crushed in high-speed rotation.
Thirdly, the temperature in the centrifugal box 31 is maintained at 37 ℃ by arranging the heating mechanism, so that the activity of stem cells can be effectively maintained, the separation operation is better performed, the efficiency is higher, and the separation time is saved.
Fourthly, the hard tissues, the stem cells and the waste liquid are separated by the filter mechanism, the group antibodies are coated on the second filter membrane layer, and the cells can be captured by the antibodies through the second filter membrane layer, so that the accuracy is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic view of a first angle configuration of the present invention;
FIG. 2 is a schematic view of a second angle according to the present invention;
FIG. 3 is a top view of the centrifugal mechanism of the present invention;
FIG. 4 is a cross-sectional view taken along A-A of FIG. 3;
FIG. 5 is a schematic diagram of the construction of the centrifuge assembly of the present invention;
FIG. 6 is a schematic view of the heating mechanism assembly of the present invention;
FIG. 7 is a top view of the filter mechanism of the present invention;
FIG. 8 is a cross-sectional view taken along line B-B of FIG. 7;
FIG. 9 is a schematic structural view of a stem cell incubator according to the present invention.
Reference numerals
The device comprises a base 1, a support table 2, a centrifugal mechanism 3, a heating mechanism 4, a filtering mechanism 5, a cleaning mechanism 6, a stem cell incubator 7, a centrifugal box 31, a centrifugal component 32, a box 311, a feed inlet 312, a discharge outlet 313, a fixing plate 321, a mounting seat 322, a first motor 323, a first belt pulley 324, a belt 325, a second belt pulley 326, a second motor 327, a first umbrella tooth 328, a second umbrella tooth 329, a screw rod 3210, a rotating rod 3211, a pushing rod 3212, a rotating plate 3213, a stirring rod 3214, a stirring blade 3215, a fixing frame 3216, a mounting frame 3217, a control panel 41, a heating ring 42, a heating pipe 43, an input pipe 51, a first water pump 52, a filtering box 53, a first filtering film layer 54, a second filtering film layer 55, a wastewater tank 56, a water tank 61, a water inlet 62, a second water pump 63, a first water pipe 64, a second water pipe 65 and a spray head 66.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention.
The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention.
All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The embodiment of the present invention provides a stem cell separation device, which is described below with reference to fig. 1 to 9, and includes a base 1, a support platform 2, a centrifugal mechanism 3, a heating mechanism 4, a filtering mechanism 5, a cleaning mechanism 6, and a stem cell incubator 7, wherein the base 1 is disposed on the ground, the support platform 2 is fixedly disposed on the base 1, the support platform 2 is used to provide a support and placement platform, the centrifugal mechanism 3 is fixedly disposed on the support platform 2, the stem cells are separated by the centrifugal mechanism 3, the heating mechanism 4 is fixedly disposed in the centrifugal mechanism 3, the temperature in the centrifugal mechanism 3 can be ensured by the heating mechanism 4, the cells are kept active, so that the separation operation is better performed, the filtering mechanism 5 is disposed at one side of the centrifugal mechanism 3, and the separated stem cells are filtered and separated, the cleaning mechanism 6 is arranged on the other side of the centrifugal mechanism 3, the separated centrifugal mechanism 3 is cleaned, impurities are prevented from affecting separation operation, the accuracy of the separation operation is guaranteed, the stem cell culture box 7 is fixedly arranged on the base 1, stem cells obtained after filtration are moved to the stem cell culture box 7, and a sterile environment is guaranteed.
The centrifugal box 31 comprises a feeding hole 312, a discharging hole 313 and a box body 311, wherein the feeding hole 312 is fixedly arranged at the top end of the box body 311, one end of the discharging hole 313 is connected with the bottom end of the box body 311, the other end of the discharging hole 313 is connected with the water delivery end of the filtering mechanism 5, and blood and decomposition enzyme can be added through the feeding hole 312.
The centrifugal assembly 32 includes a fixing plate 321, a mounting seat 322, a first motor 323, a first belt pulley 324, a belt 325, a second belt pulley 326, a second motor 327, a first bevel gear 328, a second bevel gear 329, a screw rod 3210, a rotating rod 3211, a push rod 3212, a rotating plate 3213, a stirring rod 3214, a stirring blade 3215, a fixing frame 3216, and a mounting frame 3217, the fixing plate 321 is fixedly disposed on an inner top wall of the centrifugal box 31, two mounting seats 3216 are provided, the two mounting seats 322 are respectively and fixedly disposed on two sides of the fixing plate 321, the first motor 323 is fixedly disposed on one side of the mounting seat 322, the two fixing frames 3216 are respectively and fixedly disposed on inner sides of the two mounting seats 322, the first belt pulley 324 is connected with an output end of the first motor 323, the second motor 327 is fixedly disposed on the other side of the mounting seat 322, an output end of the second motor 327 passes through the mounting seats 322 and the fixing frames 3216, the output end of the second motor 327 extends to the other side of the fixing frame 3216, the first bevel gear 328 is connected to the output end of the second motor 327, the mounting frame 3217 is disposed between the two fixing frames 3216, the second bevel gear 329 is rotatably disposed on the mounting frame 3217 and is engaged with the first bevel gear 328, the second pulley 326 is disposed at the lower side of the mounting frame 3217, the belt 325 is sleeved on the first pulley 324 and the second pulley 326, one end of the lead screw 3210 is connected to the second bevel gear 329, the other end of the lead screw 3210 penetrates through the second pulley 326 and is connected to the rotating rod 3211, one end of the rotating rod 3211 is connected to the lead screw 3210, the other end of the rotating rod 3211 is connected to the rotating plate 3213, two push rods 3212 are disposed, one end of each push rod 3212 is connected to the second pulley 326, the other end of each push rod 3212 is connected to the rotating plate 3213, the stirring rod 3214 is fixedly arranged on the rotating plate 3213, the stirring blade 3215 is fixedly arranged on the stirring rod 3214, the first motor 323 drives the first belt pulley 324 to rotate, the first belt pulley 324 drives the belt 325 to rotate, the belt 325 drives the second belt pulley 326 to rotate, the second belt pulley 326 drives the two push rods 3212 to rotate, the two push rods 3212 drive the rotating plate 3213 to rotate, thereby driving the stirring rod 3214 and the stirring blade 3215 to rotate to stir and separate the stem cells, the second motor 327 drives the first bevel gear 328 to rotate, the first bevel gear 328 and the second bevel gear 329 are engaged with each other to drive the lead screw 3210 to move, the lead screw 3210 pushes the rotating rod 3211 to move upwards, and the two push rods 3212 work simultaneously to drive the rotating plate 3213 to move up and down, thereby driving the stirring rod 3214 and the stirring blade 3215 to move up and down to separate the stem cells more effectively, produce quick flexible extrusion breakage repeatedly, avoid stereoplasm tissue scattered all around to fall to take away the soft tissue, through centrifugal component 32's setting makes stem cell separation work efficiency obtain great improvement, has also improved the practicality of this device simultaneously greatly.
The heating mechanism 4 comprises a control panel 41, a heating ring 42 and a plurality of heating pipes 43, the control panel 41 is arranged on the outer side of the centrifugal box 31, the plurality of heating pipes 43 are uniformly arranged on the heating ring 42, the input ends of the heating pipes 43 are arranged on the heating ring 42, the heating ring 42 is arranged on the inner side of the centrifugal box 31, the plurality of heating pipes 43 are all inserted into the wall of the centrifugal box 31, the heating ring 42 is heated by operating the control panel 41, so that the heating pipes 43 start to heat, the inner side wall of the centrifugal box 31 is heated by heat conduction, and the dry cells are kept at a constant temperature after entering the centrifugal box 31.
The filtering mechanism 5 comprises an input pipe 51, a first water pump 52, a filtering box 53, a first filtering membrane layer 54, a second filtering membrane layer 55 and a waste water tank 56, wherein the input end of the first water pump 52 is connected with the discharge hole 313, one end of the input pipe 51 is connected with the output end of the first water pump 52, the filtering box 53 is fixedly arranged on the base 1, the other end of the input pipe 51 is connected with the filtering box 53, the first filtering membrane layer 54 is arranged in the filtering box 53, the second filtering membrane layer 55 is arranged on the lower side of the first filtering membrane layer 54, the radius of the filtering hole of the first filtering membrane layer 54 is smaller than that of the filtering hole of the second filtering membrane layer 55, handles are arranged on one side of the first filtering membrane layer 54 and one side of the second filtering membrane layer 55, the diameter of the filtering hole of the first filtering membrane layer 54 and the diameter of the filtering hole of the second filtering membrane layer 55 are 220 and 350nm, and a group antibody is coated on the second filtering membrane layer 55, the cells can be caught by the antibodies through the second filter membrane layer 55, the wastewater tank 56 is arranged at the bottom of the filter box 53, a handle is arranged on one side of the wastewater tank 56, waste liquid is convenient to collect, the centrifuged stem cells enter the filter mechanism 5 through the first water suction pump 52, after the centrifuged stem cells pass through the first filter membrane layer 54, hard tissues are remained on the first filter membrane layer 54, the stem cells are remained on the second filter membrane layer 55, and the waste liquid flows into the wastewater tank 56.
The cleaning mechanism 6 comprises a water tank 61, a water inlet 62, a second water pump 63, a first water pipe 64, a second water pipe 65 and spray heads 66, the water tank 61 is arranged on the base 1 and is arranged on one side of the centrifugal mechanism 3, the water inlet 62 is arranged on the top of the water tank 61, water enters the water tank 61 from the water inlet 62, the first water pipe 64 is arranged in the water tank 61, one end of the first water pipe 64 penetrates through the water tank 61 and is connected with the input end of the second water pump 63, the output end of the second water pump 63 is connected with the second water pipe 65, the second water pipe 65 is arranged on the upper side of the centrifugal mechanism 3, two spray heads 66 are arranged on the second water pipe 65, and the two spray heads 66 penetrate through the centrifugal box 31 and extend to the inner side of the centrifugal box 31, so as to pour water into the water tank 61 through the water inlet 62, the water in the water tank 61 passes through the first water delivery pipe 64 and the second water delivery pipe 65 by the operation of the second water suction pump 63, and then is sprayed to the centrifugal box 31 through the spray head 66, so that the centrifugal mechanism 3 after centrifugal operation is cleaned, tissue fluid and residual cells are prevented from being attached to the centrifugal mechanism 3, and the pollution of the tissue fluid is reduced.
Stem cell incubator 7 is prior art, stem cell incubator 7 sets up one side of filter mechanism 5, stem cell incubator 7 simulates the environment of internal aseptic, suitable temperature, pH valve and certain nutrient condition, makes stem cell survive, grows, breeds and maintains primary structure and function.
The working principle is as follows: blood and lytic enzyme enter the centrifugal box 31 through the feed inlet 312, the heating mechanism 4 heats the heating ring 42 by operating the control panel 41, so that the heating pipe 43 also starts to heat, the heat conduction heats the inner wall of the centrifugal box 31, the stem cells keep constant temperature after entering the centrifugal box 31, the centrifugal assembly 32 drives the first belt pulley 324 to rotate through the first motor 323, the first belt pulley 324 drives the belt 325 to rotate, the belt 325 drives the second belt pulley 326 to rotate, the second belt pulley 326 drives the two push rods 3212 to rotate, the two push rods 3212 drive the rotating plate 3213 to rotate, so as to drive the stirring rod 3214 and the stirring blade 3215 to rotate to stir and separate the stem cells, the second motor 327 drives the first bevel gear 328 to rotate, the first bevel gear 328 and the second bevel gear 329 are meshed with each other, the lead screw 3210 is driven to move, and the lead screw 3210 pushes the rotating rod 3211 to move upwards, then work together with the two push rods 3212 to drive the rotating plate 3213 to lift, thereby driving the stirring rod 3214 and the stirring blade 3215 to lift and lower the stem cells for more effective separation, generating rapid repeated flexible extrusion and crushing, allowing the centrifuged stem cells to enter the filtering mechanism 5 through the first suction pump 52, allowing the centrifuged stem cells to pass through the first filter membrane layer 54, hard tissue remains on the first filter membrane layer 54, stem cells remain on the second filter membrane layer 55, waste fluid flows into the waste water tank 56, the second filter membrane layer 55 is moved to the stem cell incubator 7, the washing means 6 pours water into the water tank 61 through the water inlet 62, the water in the water tank 61 passes through the first water delivery pipe 64 and the second water delivery pipe 65 by the operation of the second water suction pump 63, and then is sprayed to the centrifugal box 31 by the spray head 66, so that the centrifugal mechanism 3 after centrifugal operation is cleaned.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (7)
1. The utility model provides a stem cell separation device, its characterized in that includes base (1), brace table (2), centrifugal mechanism (3), heating mechanism (4), filtering mechanism (5), wiper mechanism (6) and stem cell incubator (7), base (1) sets up subaerial, brace table (2) are fixed to be set up on base (1), centrifugal mechanism (3) are fixed to be set up on brace table (2), heating mechanism (4) are fixed to be set up in centrifugal mechanism (3), filtering mechanism (5) set up one side of centrifugal mechanism (3), wiper mechanism (6) set up the opposite side at centrifugal mechanism (3), stem cell incubator (7) are fixed to be set up on base (1).
2. A stem cell separation device according to claim 1, wherein the centrifugation means (3) is composed of a centrifugation assembly (32) and a centrifugation tank (31), the centrifugation tank (31) being fixedly arranged on the support table (2), the centrifugation assembly (32) being arranged inside the centrifugation tank (31).
3. The stem cell separation device according to claim 2, wherein the centrifugal box (31) comprises a feeding port (312), a discharging port (313) and a box body (311), the feeding port (312) is fixedly arranged at the top end of the box body (311), one end of the discharging port (313) is connected with the bottom end of the box body (311), and the other end of the discharging port (313) is connected with the water conveying end of the filtering mechanism (5).
4. The stem cell separation device of claim 3, wherein the centrifugal assembly (32) comprises a fixed plate (321), a mounting seat (322), a first motor (323), a first belt pulley (324), a belt (325), a second belt pulley (326), a second motor (327), a first bevel gear (328), a second bevel gear (329), a screw rod (3210), a rotating rod (3211), a push rod (3212), a rotating plate (3213), a stirring rod (3214), a stirring blade (3215), a fixing frame (3216) and a mounting frame (3217), the fixed plate (321) is fixedly disposed on the inner top wall of the centrifugal box (31), the mounting seat (322) is provided with two, the two mounting seats (322) are respectively fixedly disposed on two sides of the fixed plate (321), the first motor (323) is fixedly disposed on one side of the mounting seat (322), the fixing frame (3216) is provided with two, the two fixing frames (3216) are respectively and fixedly arranged at the inner sides of the two mounting seats (322), the first belt pulley (324) is connected with the output end of the first motor (323), the second motor (327) is fixedly arranged at the other side of the mounting seats (322), the output end of the second motor (327) penetrates through the mounting seats (322) and the fixing frames (3216), the output end of the second motor (327) extends to the other side of the fixing frames (3216), the first bevel gear (328) is connected with the output end of the second motor (327), the mounting frame (3217) is arranged between the two fixing frames (3216), the second bevel gear (329) is rotatably arranged on the mounting frame (3217) and is mutually engaged with the first bevel gear (328), the second belt pulley (326) is arranged at the lower side of the mounting frame (3217), and the belt (325) is sleeved on the first belt pulley (324) and the second belt pulley (326), one end of the screw rod (3210) is connected with the second bevel gear (329), the other end of the screw rod (3210) penetrates through the second belt pulley (326) and is connected with the rotating rod (3211), one end of the rotating rod (3211) is in threaded connection with the screw rod (3210), the other end of the rotating rod (3211) is connected with the rotating plate (3213), two push rods (3212) are arranged, one end of each push rod (3212) is connected with the second belt pulley (326), the other end of each push rod (3212) is connected with the rotating plate (3213), the stirring rods (3214) are fixedly arranged on the rotating plate (3213), and the stirring blades (3215) are fixedly arranged on the stirring rods (3214).
5. The stem cell separation device according to claim 4, wherein the heating mechanism (4) comprises a control panel (41), a heating ring (42) and a plurality of heating pipes (43), the control panel (41) is disposed outside the centrifugal box (31), the plurality of heating pipes (43) are uniformly disposed on the heating ring (42), the input end of the heating pipe (43) is disposed on the heating ring (42), the heating ring (42) is disposed inside the centrifugal box (31), and the plurality of heating pipes (43) are all inserted into the wall of the centrifugal box (31).
6. The stem cell separation device according to claim 5, wherein the filter mechanism (5) comprises an input pipe (51), a first water pump (52), a filter box (53), a first filter membrane layer (54), a second filter membrane layer (55) and a waste water tank (56), the input end of the first water pump (52) is connected with the discharge port (313), one end of the input pipe (51) is connected with the output end of the first water pump (52), the filter box (53) is fixedly arranged on the base (1), the other end of the input pipe (51) is connected with the filter box (53), the first filter membrane layer (54) is arranged in the filter box (53), the second filter membrane layer (55) is arranged on the lower side of the first filter membrane layer (54), and the filter pore radius of the first filter membrane layer (54) is smaller than that of the second filter membrane layer (55), and one side of each of the first filter membrane layer (54) and the second filter membrane layer (55) is provided with a handle, the diameter of the filter holes of the first filter membrane layer (54) and the second filter membrane layer (55) is 220-350nm, the wastewater tank (56) is arranged at the bottom of the filter box (53), and one side of the wastewater tank (56) is provided with a handle.
7. The stem cell separation device according to claim 6, wherein the cleaning mechanism (6) comprises a water tank (61), a water inlet (62), a second water pump (63), a first water pipe (64), a second water pipe (65) and two spray heads (66), the water tank (61) is arranged on the base (1) and is arranged at one side of the centrifugal mechanism (3), the water inlet (62) is arranged at the top of the water tank (61), the first water pipe (64) is arranged in the water tank (61), one end of the first water pipe (64) penetrates through the water tank (61) and is connected with the input end of the second water pump (63), the output end of the second water pump (63) is connected with the second water pipe (65), the second water pipe (65) is arranged at the upper side of the centrifugal mechanism (3), and two spray heads (66) are arranged, the two spray heads (66) are arranged on the second water conveying pipe (65), and the spray heads (66) penetrate through the centrifugal box (31) and extend to the inner side of the centrifugal box (31).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011013380.4A CN112080406A (en) | 2020-09-24 | 2020-09-24 | Stem cell separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011013380.4A CN112080406A (en) | 2020-09-24 | 2020-09-24 | Stem cell separation device |
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| Publication Number | Publication Date |
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| CN112080406A true CN112080406A (en) | 2020-12-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011013380.4A Withdrawn CN112080406A (en) | 2020-09-24 | 2020-09-24 | Stem cell separation device |
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| CN (1) | CN112080406A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113337395A (en) * | 2021-05-12 | 2021-09-03 | 王明辉 | Intelligent centrifugal rapid extraction device and extraction method for stem cells |
| CN113621568A (en) * | 2021-07-22 | 2021-11-09 | 江苏蒙彼利生物科技有限公司 | Umbilical cord mesenchymal stem cell serum-free culture equipment and culture method thereof |
| CN113930341A (en) * | 2021-11-16 | 2022-01-14 | 郑州大学第一附属医院 | Integrated esophageal cancer tumor cell culture and separation system |
-
2020
- 2020-09-24 CN CN202011013380.4A patent/CN112080406A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113337395A (en) * | 2021-05-12 | 2021-09-03 | 王明辉 | Intelligent centrifugal rapid extraction device and extraction method for stem cells |
| CN113621568A (en) * | 2021-07-22 | 2021-11-09 | 江苏蒙彼利生物科技有限公司 | Umbilical cord mesenchymal stem cell serum-free culture equipment and culture method thereof |
| CN113621568B (en) * | 2021-07-22 | 2024-02-13 | 江苏蒙彼利生物科技有限公司 | Umbilical cord mesenchymal stem cell serum-free culture equipment and culture method thereof |
| CN113930341A (en) * | 2021-11-16 | 2022-01-14 | 郑州大学第一附属医院 | Integrated esophageal cancer tumor cell culture and separation system |
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Application publication date: 20201215 |