CN113930341A - Integrated esophageal cancer tumor cell culture and separation system - Google Patents
Integrated esophageal cancer tumor cell culture and separation system Download PDFInfo
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- CN113930341A CN113930341A CN202111351934.6A CN202111351934A CN113930341A CN 113930341 A CN113930341 A CN 113930341A CN 202111351934 A CN202111351934 A CN 202111351934A CN 113930341 A CN113930341 A CN 113930341A
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Abstract
The invention discloses an integrated esophageal cancer tumor cell culture and separation system, which comprises four rectangular stand columns, wherein the upper ends of the four stand columns are fixedly connected with a driving installation mechanism together, and the interior of the driving installation mechanism, which is close to the lower end, is fixedly connected with a culture mechanism, so that the integrated esophageal cancer tumor cell culture and separation system has the following beneficial effects that: first driving motor, second drive wheel and belt second drive wheel and actuating lever rotate, first slider slides in first ring channel inside and rotates the stable incubator rotation that drives, centrifugal force breaks away from the partial cell of incubator inside wall, second driving motor and output pole drive the centrifugal plate and rotate, increase the centrifugal force rotation of culture solution, increase and break away from efficiency, first gear conveniently drives four second gears and rotates, be convenient for drive four bull sticks and four rotary drums and rotate, four rotary drums drive a plurality of separation fluff simultaneously and rotate, clean the cell that half breaks away from of incubator inside wall down fast, increase the cell separation speed of incubator.
Description
Technical Field
The invention relates to esophageal cancer tumor cell culture, in particular to an integrated esophageal cancer tumor cell culture and separation system, and belongs to the technical field of medical treatment.
Background
Esophageal cancer is a malignant tumor that occurs in the esophagus and is derived from esophageal epithelial cells. The prevalence rate and the mortality rate of esophageal cancer in China are ranked in the fifth position worldwide, accounting for about 55%, in 2018, the number of new cases of esophageal cancer in China is estimated to be 30.7 thousands, and the morbidity rate is ranked in the sixth position; the number of deaths is expected to be 28.3 thousands of people, the fourth position.
In order to treat and research esophageal cancer, the medical part often can be cultured the tumor cell of esophageal cancer, thereby make things convenient for later stage research, but at present can not be fine when culturing esophageal cancer tumor cell effectively separate low active cell and active cell, all separate through the manual work, separation efficiency is low, and the separating speed is slow, and low active tumor cell can the adhesion on the culture dish inner wall when separating, it is very inconvenient to clear up, inconvenient medical use, and inside culture solution needs regular change, it is more troublesome now to change culture solution, can not discharge fast.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides an integrated esophageal cancer tumor cell culture and separation system.
In order to achieve the purpose, the invention adopts the following technical scheme:
the designed integrated esophageal cancer tumor cell culture separation system comprises four rectangular stand columns, wherein the upper ends of the four stand columns are fixedly connected with a driving installation mechanism together, the interior of the driving installation mechanism, which is close to the lower end, is fixedly connected with a culture mechanism, the inner side wall of the culture mechanism, which is close to the middle part, is fixedly connected with a centrifugal mechanism, the inner side wall of the top end of the culture mechanism, which is positioned at two sides of the centrifugal mechanism, is rotatably connected with two separation mechanisms, and the upper end of one side of the driving installation mechanism is fixedly connected with a drawing-off mechanism;
the driving installation mechanism comprises an installation block, two sides of the lower surface of the installation block are fixedly connected to the upper ends of four upright posts, the installation block is arranged in a cylindrical shape, a circular installation groove is formed in the upper end of the installation block, a first installation hole is formed in the bottom inner groove wall of the installation groove close to the middle position in a penetrating mode, a first bearing is fixedly sleeved in the first installation hole, a first annular groove is formed in the bottom inner groove wall of the installation groove at two sides of the first installation hole in an annular mode, a second annular groove is formed in the outer side wall of the top end of the installation block in an annular mode, a driving rod is fixedly sleeved in the first bearing, the lower end of the driving rod penetrates through the first bearing and extends to the lower end of the installation block, and the upper end of the driving rod penetrates through the first bearing and extends into the installation block, the upper end of the driving rod penetrates through the first bearing and extends into the mounting groove, a first driving wheel is fixedly sleeved at the outer end, close to the lower end, of the rod wall of the driving rod at the lower end of the mounting block, a mounting frame is fixedly connected to the outer side wall of the bottom end of the mounting block at one side of the first driving wheel, a first driving motor is fixedly sleeved on the inner side wall of the mounting frame, the output end of the first driving motor is fixedly connected with a second driving wheel through a coupler, the first driving wheel and the second driving wheel are horizontally arranged, and a belt is jointly connected between the second driving wheel and the first driving wheel in a driving mode;
the culture mechanism comprises a culture box which is arranged in a transparent shape and is arranged in a cylindrical shape, the culture box is fixedly connected to a rod wall of a driving rod which is positioned in a mounting groove and is close to the upper end position, a second mounting hole is formed in the outer wall of the upper end of the culture box and is close to the middle position in a penetrating manner, a second bearing is fixedly sleeved in the second mounting hole, first sliding blocks are fixedly connected to the outer walls of the lower surfaces of the two sides of the culture box and the corresponding positions of the first annular grooves, the two first sliding blocks are symmetrically arranged and fixedly connected to the two sides of the lower surface of the culture box, the lower ends of the two first sliding blocks penetrate through the notches of the first annular grooves, the lower ends of the two first sliding blocks extend into the first annular grooves, the lower ends of the two first sliding blocks are slidably connected in the first annular grooves, and an L-shaped plate is fixedly connected to the outer wall of the upper end of the culture box, which is close to the middle position, and the L-shaped plate is positioned at the second mounting hole and the upper end of the second bearing.
Preferably, the centrifugal mechanism includes a second driving motor, second driving motor fixed connection is at L shaped plate lower extreme, just second driving motor output end fixed connection has an output pole, the output pole lower extreme rotates to be connected inside the second bearing and extend to the incubator in, the pole wall that the output pole is close to the upper end position is fixed to be cup jointed a first gear, just four centrifugal plates that are the symmetry and set up of output pole lower extreme pole wall fixed connection.
Preferably, the separating mechanism comprises four third bearings, four the third bearings are in an annular shape and are fixedly connected to the inner wall of the top end of the incubator, four rotating rods are fixedly sleeved at the lower ends of the third bearings, four second gears are fixedly sleeved on the rod walls of the rotating rods close to the upper end positions, and four second gears are meshed and connected to the periphery of the first gears.
Preferably, the rod wall of the lower end of each of the four rotating rods is fixedly connected with a sleeve, and the outer parts of the four sleeves are fixedly connected with a plurality of separated bristles.
Preferably, take out from the mechanism and include a water pump, water pump fixed connection is in incubator upper surface one side, an inlet pipe of water pump one side fixedly connected with, the inlet pipe lower extreme runs through the incubator and extends to in the incubator.
Preferably, one side of the water pump far away from the feeding pipe is fixedly connected with a discharging pipe, and one side of the discharging pipe far away from the water pump extends to the outside of the mounting block.
Preferably, the lower end of the discharge pipe and the corresponding position of the second annular groove are fixedly connected with a second sliding block, and the lower end of the second sliding block extends into the second annular groove and is connected in the second annular groove in a sliding manner.
Preferably, one side of the discharge pipe, which is far away from the water pump, is rotatably provided with a valve, and the pipe opening of the discharge pipe is in threaded connection with a first sealing cover.
Preferably, one side of the incubator far away from the water pump is fixedly connected with an inlet and outlet pipe, the lower end of the inlet and outlet pipe penetrates through the incubator and extends into the incubator, and the upper end of the incubator is in threaded connection with a second sealing cover.
Preferably, the lower ends of the four upright posts are fixedly connected with a bottom plate.
The integrated esophageal cancer tumor cell culture and separation system provided by the invention has the beneficial effects that: 1. through setting up first driving motor and second drive wheel convenience and drive second drive wheel and actuating lever rotation under the effect of belt and first bearing to can slide the convenient stable incubator that drives of rotation in first ring channel through first slider, thereby break away from the partial cell of incubator inside wall through centrifugal force and get off, second driving motor and output lever drive the centrifugal plate rotation simultaneously, the centrifugal force that further increases the culture solution rotates, increase and break away from efficiency.
1. Conveniently drive four second gear rotations through setting up first gear, be convenient for drive four bull sticks and four rotary drums and rotate, four rotary drums drive a plurality of separation fluff rotations simultaneously, and the cell that conveniently breaks away from the incubator inside wall cleans fast down, further increases the cell separation speed of incubator.
2. Through setting up the business turn over pipe convenient entering culture solution and esophagus cancer cell, discharge the culture solution through water pump, inlet pipe and discharging pipe convenience simultaneously fast to discharge the culture solution of incubator inside fast, convenient change, convenient to use fast.
Drawings
FIG. 1 is a schematic view of an integrated esophageal cancer tumor cell culture and isolation system according to the present invention;
FIG. 2 is an enlarged schematic structural view of part A in FIG. 1 of the integrated esophageal cancer tumor cell culturing and separating system provided by the present invention;
FIG. 3 is an enlarged schematic structural view of part B in FIG. 1 of the integrated esophageal cancer tumor cell culturing and separating system provided by the present invention;
FIG. 4 is an enlarged schematic structural view of a portion C in FIG. 1 of the integrated esophageal cancer tumor cell culturing and separating system provided by the present invention;
FIG. 5 is a schematic sectional structure view along the direction D-D in FIG. 1 of the integrated esophageal cancer tumor cell culturing and separating system provided by the present invention.
FIG. 6 is a schematic cross-sectional view along the direction E-E in FIG. 1 of the integrated esophageal cancer tumor cell culturing and separating system provided by the present invention;
FIG. 7 is a schematic sectional view of the integrated esophageal cancer tumor cell culturing and separating system of the present invention, the sectional view being taken along the direction F-F in FIG. 1;
FIG. 8 is a schematic cross-sectional view along the G-G direction in FIG. 1 of the integrated esophageal cancer tumor cell culturing and separating system provided by the present invention;
FIG. 9 is a schematic cross-sectional view of the integrated esophageal cancer tumor cell culture and isolation system according to the present invention;
fig. 10 is a schematic structural view of the integrated esophageal cancer tumor cell culture and separation system provided by the invention and the extraction mechanism in fig. 9.
In the figure: a column 1,
Drive mounting mechanism 2, mounting block 21, mounting groove 22, first mounting hole 23, first bearing 24, first annular groove 25, second annular groove 26, driving rod 27, first driving wheel 28, mounting frame 29, first driving motor 210, second driving wheel 211, belt 212, and belt-driven mechanism,
The culture mechanism 3, the incubator 31, the second mounting hole 32, the second bearing 33, the first slider 34, the L-shaped plate 35, and the like,
A centrifugal mechanism 4, a second driving motor 41, an output rod 42, a first gear 43, a centrifugal plate 44,
A separating mechanism 5, a third bearing 51, a rotating rod 52, a second gear 53, a sleeve 54, a separating fluff 55,
A pumping mechanism 6, a water pump 61, a feeding pipe 62, a discharging pipe 63, a second slide block 64, a valve 65, a first sealing cover 66,
An inlet pipe 7, a second sealing cover 8 and a bottom plate 9.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-10, the integrated esophageal cancer tumor cell culture separation system comprises four rectangular upright posts 1, wherein the upper ends of the four upright posts 1 are jointly and fixedly connected with a driving installation mechanism 2, the interior of the driving installation mechanism 2 close to the lower end position is fixedly connected with a culture mechanism 3, the inner side wall of the culture mechanism 3 close to the middle position is fixedly connected with a centrifugal mechanism 4, the inner side walls of the top ends of the culture mechanisms 3 at two sides of the centrifugal mechanism 4 are rotatably connected with two separation mechanisms 5, and the upper end of one side of the driving installation mechanism 2 is fixedly connected with a pumping-out mechanism 6;
the driving installation mechanism 2 comprises an installation block 21, two sides of the lower surface of the installation block 21 are fixedly connected with the upper ends of the four upright posts 1, the installation block 21 is arranged in a cylindrical shape, an installation groove 22 arranged in a circular shape is formed in the upper end of the installation block 21, a first installation hole 23 is formed in the installation groove 22 in a penetrating manner and is close to the bottom end inner groove wall of the middle position, a first bearing 24 is fixedly sleeved in the first installation hole 23, a first annular groove 25 arranged in an annular shape is formed in the bottom end inner groove wall of the installation groove 22 positioned at two sides of the first installation hole 23, a second annular groove 26 arranged in an annular shape is formed in the outer side wall of the top end of the installation block 21, a driving rod 27 is fixedly sleeved in the first bearing 24, the lower end of the driving rod 27 penetrates through the first bearing 24 and extends to the lower end of the installation block 21, and the upper end of the driving rod 27 penetrates through the first bearing 24 and extends to the installation block 21, the upper end of the driving rod 27 penetrates through the first bearing 24 and extends into the mounting groove 22, the outer end of the rod wall of the driving rod 27, close to the lower end, of the lower end of the mounting block 21 is fixedly sleeved with a first driving wheel 28, the outer side wall of the bottom end of the mounting block 21, located on one side of the first driving wheel 28, is fixedly connected with a mounting frame 29, the inner side wall of the mounting frame 29 is fixedly sleeved with a first driving motor 210, the output end of the first driving motor 210 is fixedly connected with a second driving wheel 211 through a coupler, the first driving wheel 28 and the second driving wheel 211 are horizontally arranged, and a belt 212 is jointly connected between the second driving wheel 211 and the first driving wheel 28 in a driving mode;
the culture mechanism 3 comprises a culture box 31 which is arranged in a transparent shape, the culture box 31 is arranged in a cylindrical shape, the culture box 31 is fixedly connected to the rod wall of the driving rod 27 which is positioned in the installation groove 22 and is close to the upper end, the outer box wall of the upper end of the culture box 31 and is close to the middle position is provided with a second installation hole 32 in a penetrating way, a second bearing 33 is fixedly sleeved in the second installation hole 32, the outer box walls on the lower surfaces of the two sides of the culture box 31 and the corresponding position of the first annular groove 25 are fixedly connected with a first sliding block 34, the two first sliding blocks 34 are symmetrically arranged and fixedly connected to the two sides of the lower surface of the culture box 31, the lower ends of the two first sliding blocks 34 penetrate through the notches of the first annular groove 25, the lower ends of the two first sliding blocks 34 extend into the first annular groove 25, the lower ends of the two first sliding blocks 34 are connected in the first annular groove 25 in a sliding way, the outer box wall of the upper end of the culture box 31 and is close to the middle position is fixedly connected with an L-shaped plate 35, and an L-shaped plate 35 is positioned at the upper ends of the second mounting hole 32 and the second bearing 33.
Centrifugal mechanism 4 includes a second driving motor 41, second driving motor 41 fixed connection is at L shaped plate 35 lower extreme, and output rod 42 of second driving motor 41 output end fixed connection, output rod 42 lower extreme rotates to be connected inside second bearing 33 and extend to incubator 31 in, output rod 42 has cup jointed a first gear 43 near the pole wall of upper end position is fixed, and output rod 42 lower extreme pole wall fixed connection has four centrifugal plates 44 that are the symmetry and set up, be favorable to conveniently driving output rod 42 and first gear 43 through second driving motor 41 and rotate, thereby can further increase incubator 31's centrifugal force, increase separation efficiency.
The sleeve 54 is fixedly connected to the rod wall of the lower end of each of the four rotating rods 52, the plurality of separation bristles 55 are fixedly connected to the outer portions of the four sleeves 54, the four sleeves 54 are favorable for conveniently driving the plurality of separation bristles 55 to rotate, and semi-separated cells on the inner wall of the incubator 31 can be conveniently taken away.
Draw from mechanism 6 and include a water pump 61, water pump 61 fixed connection is in incubator 31 upper surface one side, inlet pipe 62 of water pump 61 one side fixedly connected with, and inlet pipe 62 lower extreme runs through incubator 31 and extends to in the incubator 31, is favorable to conveniently driving inlet pipe 62 through water pump 61 and takes out the cell and the culture solution that activity is low.
One side fixedly connected with discharging pipe 63 that inlet pipe 62 was kept away from to water pump 61, outside one side that water pump 61 was kept away from to discharging pipe 63 extended to installation piece 21, was favorable to conveniently discharging the culture solution fast through discharging pipe 63, and the discharge efficiency is high.
The corresponding position fixedly connected with second slider 64 of discharging pipe 63 lower extreme and second annular groove 26, second slider 64 lower extreme extend to in the second annular groove 26 and sliding connection in second annular groove 26, are favorable to guaranteeing stability, the security is high when discharging pipe 63 rotates through second slider 64 convenience.
One side of the discharging pipe 63, which is far away from the water pump 61, is rotatably provided with a valve 65, the pipe opening of the discharging pipe 63 is in threaded connection with a first sealing cover 66, the discharging speed of the discharging pipe 63 can be conveniently controlled through the valve 65, and meanwhile, the discharging speed of the discharging pipe 63 is increased through the first sealing cover 66.
One side fixedly connected with of water pump 61 is kept away from to incubator 31 advances exit tube 7, advances exit tube 7 lower extreme and runs through incubator 31 and extend to incubator 31 in, and incubator 31 upper end threaded connection has a sealed lid 8 of second, is favorable to conveniently adjusting cell and culture solution through advance exit tube 7, increases the leakproofness of advance exit tube 7 through the sealed lid 8 of second.
Four 1 lower extremes of stand fixedly connected with a bottom plate 9 is favorable to making things convenient for the stable stay installation piece 21 through four bottom plates 9, is convenient for increase support stability.
The working principle is as follows: when the cell culture device is used, the mounting blocks 21 are stably supported by four upright posts 1 and four bottom plates 9, then the second sealing cover 8 is opened, a culture solution and cells are injected into the incubator 31 through the inlet and outlet pipes 7, then the second sealing cover 8 is screwed on by threads for cell culture, when cells with low activity need to be separated out, the first driving motor 210 and the second driving motor 41 are started, the output end of the first driving motor 210 drives the second driving wheel 211 to rotate, the second driving wheel 211 drives the first driving wheel 28 to rotate through the belt 212, the first driving wheel 28 drives the driving rod 27 to rotate through the first mounting hole 23 and the first bearing 24, the driving rod 27 synchronously drives the incubator 31 to rotate, the incubator 31 drives the first sliding block 34 to rotate in the first annular groove 25, the rotation stability of the incubator 31 is improved, and simultaneously the output end of the second driving motor 41 drives the output rod 42 to rotate through the second mounting hole 32 and the second bearing 33, the lower end of the output rod 42 drives the first gear 43 and the four centrifugal plates 44 to rotate, the four centrifugal plates 44 further increase the centrifugal force of the culture solution in the incubator 31, at this time, a part of low-activity cells can be separated from the wall of the incubator 31, meanwhile, the first gear 43 is meshed to drive the four second gears 53 to rotate, the four second gears 53 drive the four rotating rods 52 to rotate through the third bearing 51, the four rotating rods 52 drive the four sleeves 54 to rotate, the sleeves 54 drive the plurality of separated soft bristles 55 to rotate continuously, at this time, the cells in a semi-separated state with the incubator 31 can be in continuous contact with the separated soft bristles 55, so as to be separated quickly, when the incubator 31 rotates, the water pump 61 drives the discharge pipe 63 to rotate, the second sliding block 64 at the lower end of the discharge pipe 63 can rotate in the second annular groove 26, so as to increase the safety, at this time, the valve 65 and the first sealing cover 66 are opened, and then the water pump 61 is started, the water pump 61 pumps out the culture solution with low activity cells through the feeding pipe 62, and then discharges the culture solution through the discharging pipe 63, when the high activity cells need to be extracted, the cells on the inner wall of the culture box 31 can be scraped through the inlet and outlet pipe 7.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (10)
1. Integral type esophagus cancer tumor cell culture piece-rate system, its characterized in that is including four stand (1) that are the rectangle setting, four drive installation mechanism (2) of the common fixedly connected with in stand (1) upper end, drive installation mechanism (2) are close to one of the inside fixedly connected with of lower extreme position and cultivate mechanism (3), cultivate mechanism (3) and be close to centrifugal mechanism (4) of inside wall fixedly connected with of middle part position, be located the cultivation mechanism (3) top inside wall of centrifugal mechanism (4) both sides rotates and is connected with two separating mechanism (5), just one of drive installation mechanism (2) one side upper end fixedly connected with takes out from mechanism (6).
2. The integrated esophageal cancer tumor cell culture and isolation system of claim 1,
the driving installation mechanism (2) comprises an installation block (21), two sides of the lower surface of the installation block (21) are fixedly connected to the upper ends of the four stand columns (1), the installation block (21) is in a cylindrical shape, an installation groove (22) which is in a circular shape is formed in the upper end of the installation block (21), a first installation hole (23) is formed in the bottom inner groove wall of the installation groove (22) close to the middle position in a penetrating mode, a first bearing (24) is fixedly sleeved in the first installation hole (23), a first annular groove (25) which is in an annular shape is formed in the bottom inner groove wall of the installation groove (22) located on two sides of the first installation hole (23), a second annular groove (26) which is in an annular shape is formed in the outer side wall of the top end of the installation block (21), and a driving rod (27) is fixedly sleeved in the first bearing (24), the utility model discloses a mounting block, including drive lever (27), drive lever (27) lower extreme, drive lever (27) upper end pass first bearing (24) and extend to in installation piece (21), drive lever (27) upper end is passed first bearing (24) and is extended to in mounting groove (22), is located drive lever (27) of installation piece (21) lower extreme are close to the fixed cover of pole wall outer end of lower extreme position and are had a first drive wheel (28), and are located installation piece (21) bottom lateral wall fixedly connected with one mounting bracket (29) of first drive wheel (28) one side, the fixed cover of mounting bracket (29) inside wall has a first driving motor (210), first driving motor (210) output passes through shaft coupling fixedly connected with a second drive wheel (211), first drive wheel (28) and second drive wheel (211) are the horizontal setting, and a belt (212) is connected between the second transmission wheel (211) and the first transmission wheel (28) in a transmission way.
3. The integrated esophageal cancer tumor cell culture and isolation system of claim 2,
cultivate mechanism (3) including one and be incubator (31) that the transparent form set up, just incubator (31) are cylindrical setting, incubator (31) fixed connection is on being located the pole wall that actuating lever (27) inside mounting groove (22) are close to the upper end position, incubator (31) upper end is close to the outer wall of middle part position and runs through and has seted up a second mounting hole (32), just the inside fixed cover of second mounting hole (32) has a second bearing (33), incubator (31) both sides lower surface outer wall and first ring channel (25) corresponding position equal fixedly connected with a first slider (34), two first slider (34) are symmetry and set up fixed connection in incubator (31) lower surface both sides, and two first slider (34) lower extreme passes first ring channel (25) notch, two first slider (34) lower extreme extends to inside first ring channel (25), and two first slider (34) lower extreme sliding connection is in first ring channel (25), outer box wall fixedly connected with L shaped plate (35) that incubator (31) upper end is close to the middle part position, just L shaped plate (35) are located second mounting hole (32) and second bearing (33) upper end.
4. The integrated esophageal cancer tumor cell culture and isolation system of claim 3,
the centrifugal mechanism (4) comprises a second driving motor (41), the second driving motor (41) is fixedly connected to the lower end of the L-shaped plate (35), an output rod (42) is fixedly connected to the output end of the second driving motor (41), the lower end of the output rod (42) is rotatably connected to the inside of the second bearing (33) and extends into the incubator (31), a first gear (43) is fixedly sleeved on the rod wall of the output rod (42) close to the upper end of the output rod, and four centrifugal plates (44) which are symmetrically arranged are fixedly connected to the rod wall of the lower end of the output rod (42);
the separating mechanism (5) comprises four third bearings (51), the four third bearings (51) are annularly arranged and fixedly connected to the inner box wall at the top end of the incubator (31), the lower ends of the four third bearings (51) are fixedly sleeved with a rotating rod (52), the rod walls of the four rotating rods (52) close to the upper end are fixedly sleeved with a second gear (53), and the four second gears (53) are meshed and connected to the periphery of the first gear (43); the lower end rod walls of the four rotating rods (52) are fixedly connected with a sleeve (54), and the outer parts of the four sleeves (54) are fixedly connected with a plurality of separated soft bristles (55).
5. The integrated esophageal cancer tumor cell culture and isolation system of claim 4,
draw from mechanism (6) and include a water pump (61), water pump (61) fixed connection is in incubator (31) upper surface one side, inlet pipe (62) of water pump (61) one side fixedly connected with, inlet pipe (62) lower extreme runs through incubator (31) and extends to in incubator (31).
6. The integrated esophageal cancer tumor cell culture and isolation system according to claim 5, wherein a discharge pipe (63) is fixedly connected to one side of the water pump (61) away from the feed pipe (62), and one side of the discharge pipe (63) away from the water pump (61) extends out of the mounting block (21).
7. The integrated esophageal cancer tumor cell culture and isolation system according to claim 6, wherein a second sliding block (64) is fixedly connected to the lower end of the discharge pipe (63) and the second annular groove (26) at a position corresponding to the lower end of the discharge pipe, and the lower end of the second sliding block (64) extends into the second annular groove (26) and is slidably connected in the second annular groove (26).
8. The integrated esophageal cancer tumor cell culture and isolation system according to claim 7, wherein a valve (65) is rotatably mounted on a side of the discharge pipe (63) away from the water pump (61), and a first sealing cover (66) is screwed on an opening of the discharge pipe (63).
9. The integrated esophageal cancer tumor cell culture and isolation system according to claim 8, wherein an inlet and outlet pipe (7) is fixedly connected to one side of the incubator (31) away from the water pump (61), the lower end of the inlet and outlet pipe (7) penetrates through the incubator (31) and extends into the incubator (31), and a second sealing cover (8) is connected to the upper end of the incubator (31) in a threaded manner.
10. The integrated esophageal cancer tumor cell culture and isolation system according to claim 9, wherein a bottom plate (9) is fixedly connected to the lower ends of the four upright posts (1).
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