CN110540931A - Culture apparatus for cell separation test - Google Patents
Culture apparatus for cell separation test Download PDFInfo
- Publication number
- CN110540931A CN110540931A CN201910731916.7A CN201910731916A CN110540931A CN 110540931 A CN110540931 A CN 110540931A CN 201910731916 A CN201910731916 A CN 201910731916A CN 110540931 A CN110540931 A CN 110540931A
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- CN
- China
- Prior art keywords
- equipment box
- box body
- culture
- culture apparatus
- rotating
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Abstract
The invention discloses a culture device for a cell separation test, which comprises a device base, an equipment box body and a culture disc, wherein a rotating block is connected to the outer side of the device base, a supporting column is connected to the position right above the device base, an internal threaded rod is nested in the supporting column, a driving motor is connected to the position right above the equipment box body, a sealing box door is hinged and fixed to the outer side of the equipment box body, a second lead screw is connected to the position right below the equipment box body, a dust cover is connected to the position right below the second lead screw, a sealing ring is connected to the position right below the culture disc, a connecting column is connected to the position right below the sealing ring, a jack is formed in the upper surface of the culture disc, and a bevel gear is connected to the outer side. This culture apparatus for cell separation test adopts internal threaded rod and first lead screw, drives the internal threaded rod through first lead screw and rotates the lift, is convenient for according to the height of user's altitude mixture control equipment box.
Description
Technical Field
The invention relates to the technical field of cell separation equipment, in particular to a culture device for cell separation tests.
Background
Along with the stricter requirements on quality control, the requirements of constant temperature and humidity biological laboratories are larger and larger, the application field is wider and wider, the cell separation is mainly to extract a single individual through a capillary tube and transfer and clean the single individual in a large amount of sterilized culture media for several times to remove the pollution of smaller microorganisms, and the single cell separation method is a method for directly separating a single cell from a material to be separated and culturing the single cell to obtain pure culture.
Present culture apparatus is metal integrated structure, is difficult to according to user's height adjusting device self height, increases the trouble of user's daily operation to at separation and cultivation in-process, be difficult to carry out sealed processing to the cell after the separation, lead to the dust to be infected with on the cell, influence cell culture's effect.
Disclosure of Invention
The invention aims to provide a culture device for cell separation tests, which solves the problems that the existing culture device proposed in the background art is of a metal integrated structure, the height of the device is difficult to adjust according to the height of a user, and in the separation and culture processes, the cells are difficult to seal after separation, so that dust is polluted on the cells.
In order to achieve the purpose, the invention provides the following technical scheme: a culture device for cell separation tests comprises a device base, an equipment box body and a culture disc, wherein the outer side of the device base is connected with a rotating block, a support column is connected to the position right above the device base, an inner threaded rod is nested in the support column, a driving motor is connected to the position right above the equipment box body, a sealing box door is hinged and fixed to the outer side of the equipment box body, a second lead screw is connected to the position right below the equipment box body, a dust cover is connected to the position right below the second lead screw, a sealing ring is connected to the position right below the culture disc, a connecting column is connected to the position right below the sealing ring, a jack is formed in the upper surface of the culture disc, the outer side of the rotating block is connected with a conical tooth, the outer side of the conical tooth is connected with a first lead screw, a refrigerating piece is arranged on the inner wall of the equipment box, the cooling fan is characterized in that a sliding block is connected under the rectangular sliding rail, a rotating gear is connected under the sliding block, and cooling fan blades are connected under the rotating gear.
Preferably, the diameter of support column is less than the diameter of internal thread pole, and the equipment box passes through internal thread pole, rotary gear and rotatory piece and constitutes elevation structure.
preferably, the length of the second threaded rod is smaller than that of the dust cover, the dust cover is parallel to the device base, and the device base is of a hollow rectangular structure.
Preferably, the dust cover and the second screw rod form a rotating structure through a rectangular sliding rail, a sliding block and rotating gears, and the number of the rotating gears is 2.
preferably, the culture disc and the sealing ring are bonded into an integrated structure, and connecting columns are distributed at the bottom of the sealing ring at equal intervals.
Preferably, the sealing ring is of an annular transparent structure, the diameter of the sealing ring is smaller than that of the culture disc, and the 2 groups of culture discs form a disassembly and assembly structure through the connecting columns and the jacks.
Preferably, the first screw rod and the equipment box body are mutually perpendicular, and the first screw rod and the rotating block form a rotating structure through a rotating gear.
Preferably, the sliding block and the first screw rod are connected through a bearing, the first screw rod and the dust cover form a sliding structure through the sliding block and the rectangular sliding rail, and the length of the rectangular sliding rail is greater than half of the length of the equipment box body.
Preferably, the cooling fan blades and the refrigerating fins are symmetrically distributed about the central line of the equipment box body, and the bottom of the equipment box body is of an open structure.
Compared with the prior art, the invention has the beneficial effects that: the culture device for the cell isolation test is provided with a culture chamber,
1. The inner threaded rod and the first lead screw are adopted, and the inner threaded rod is driven to rotate and lift through the first lead screw, so that on one hand, the height of the equipment box body is conveniently adjusted according to the height of a user, on the other hand, the distance between the cooling fan blades and the culture disc is adjusted, the cold storage temperature of cells is further adjusted, and the convenience of daily cell separation operation is improved;
2. The rotating gear and the sliding block are adopted, the screw rod is driven to rotate through the rotating gear, the dust cover is driven to rotate through the screw rod, the height of the dust cover is further adjusted, shielding is facilitated in the cell separation process, the rotating gear is driven to horizontally move through the sliding block, and corresponding adjustment and movement are facilitated;
3. The bevel gear and the second screw rod are adopted, the transmission direction of the rotating force is changed through the bevel gear, and then the motion and the power between two intersecting shafts are transmitted, the automation degree of the self operation of the lifting device is further improved, meanwhile, the second screw rod is utilized to drive the dust cover to vertically lift, and the automation degree of the use of the lifting device is improved.
Drawings
FIG. 1 is a schematic front view of the present invention;
FIG. 2 is a schematic view of the internal structure of the apparatus case of the present invention;
FIG. 3 is a schematic top view of the apparatus case of the present invention;
FIG. 4 is a schematic bottom view of the sealing ring of the present invention;
FIG. 5 is a schematic top view of the culture disc of the present invention.
In the figure: 1. a device base; 2. rotating the block; 3. a support pillar; 4. an inner threaded rod; 5. an equipment box body; 6. a drive motor; 7. sealing the box door; 8. a second lead screw; 9. a dust cover; 10. cultivating the disc; 11. a seal ring; 12. connecting columns; 13. a jack; 14. conical teeth; 15. a first lead screw; 16. a refrigeration plate; 17. a rectangular slide rail; 18. a slider; 19. a rotating gear; 20. and cooling the fan blades.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, the present invention provides a technical solution: a culture device for cell separation tests comprises a device base 1, a rotating block 2, a supporting column 3, an inner threaded rod 4, an equipment box body 5, a driving motor 6, a sealing box door 7, a second screw rod 8, a dust cover 9, a culture disc 10, a sealing ring 11, a connecting column 12, a jack 13, a bevel gear 14, a first screw rod 15, a refrigerating sheet 16, a rectangular sliding rail 17, a sliding block 18, a rotating gear 19 and a cooling fan blade 20, wherein the outer side of the device base 1 is connected with the rotating block 2, the supporting column 3 is connected right above the device base 1, the inner threaded rod 4 is nested inside the supporting column 3, the driving motor 6 is connected right above the equipment box body 5, the sealing box door 7 is hinged and fixed on the outer side of the equipment box body 5, the second screw rod 8 is connected right below the equipment box body 5, the dust cover 9 is connected right below the second screw rod 8, the sealing ring 11 is connected right below the, and a connecting column 12 is connected right below the sealing ring 11, an inserting hole 13 is formed in the upper surface of the cultivation disc 10, a bevel gear 14 is connected to the outer side of the rotating block 2, a first screw rod 15 is connected to the outer side of the bevel gear 14, a refrigerating sheet 16 is installed and arranged on the inner wall of the equipment box body 5, a rectangular sliding rail 17 is connected right above the equipment box body 5, a sliding block 18 is connected right below the rectangular sliding rail 17, a rotating gear 19 is connected right below the sliding block 18, and cooling fan blades 20 are connected right below the rotating gear 19.
The diameter of support column 3 is less than the diameter of internal threaded rod 4, and equipment box 5 constitutes elevation structure through internal threaded rod 4, rotary gear 19 and rotatory piece 2, is convenient for adjust the height of equipment box 5 according to the user demand, increases the convenience of the daily regulation of device.
The length of second lead screw 8 is less than the length of dust cover 9, and dust cover 9 and device base 1 are parallel to each other to the inside of device base 1 adopts hollow rectangle structure, shelters from the cell separation ware top in the operation process through dust cover 9, ensures the cleanliness factor in the separation process.
The dust cover 9 and the second screw rod 8 form a rotating structure through a rectangular sliding rail 17, a sliding block 18 and rotating gears 19, and the number of the rotating gears 19 is 2 groups, so that the height of the dust cover 9 can be conveniently adjusted according to the separation operation requirement, and the cleanliness in the cell separation process is further ensured.
Cultivate disc 10 and seal ring 11 bonding structure as an organic whole, and the equidistant distribution in bottom of seal ring 11 has spliced pole 12, cultivates disc 10 through spliced pole 12 to the multiunit and connects, promotes the convenience of cultivateing disc 10 and fixing the culture dish.
sealing ring 11 is annular transparent structure, and sealing ring 11's diameter is less than the diameter of cultivateing disc 10 to 2 groups cultivate disc 10 and constitute through spliced pole 12 and jack 13 and dismantle mounting structure, are convenient for carry out classified storage with the cell after the separation, promote the convenience of cell daily culture.
the first screw rod 15 and the equipment box body 5 are mutually perpendicular, the first screw rod 15 and the rotating block 2 form a rotating structure through the rotating gear 19, and when the equipment below is cooled through the cooling fan blades 20 inside the equipment box body 5, the first screw rod 15 drives the equipment box body 5 to vertically lift.
The sliding block 18 is connected with the first screw rod 15 through a bearing, the first screw rod 15 and the dust cover 9 form a sliding structure through the sliding block 18 and the rectangular sliding rail 17, the length of the rectangular sliding rail 17 is larger than half of the length of the equipment box body 5, the position of the first screw rod 15 is convenient to adjust according to use requirements, the first screw rod 15 is driven to rotate through the rotating gear 19, and convenience in adjusting the dust cover 9 is guaranteed.
The cooling fan blades 20 and the refrigerating pieces 16 are symmetrically distributed about the central line of the equipment box body 5, the bottom of the equipment box body 5 is of an open structure, the cultivation disc 10 is cooled through the cooling fan blades 20 and the refrigerating pieces 16, and the generated cooled wind power is output through the equipment box body 5.
The working principle is as follows: when the culture device for the cell separation test is used, as shown in fig. 1 and 2, an operator places cells to be separated on a device base 1, opens a refrigerating sheet 16 and a driving motor 6, cools the cells on the surface of the device base 1 through the refrigerating sheet 16, then drives a cooling fan blade 20 and a rotating gear 19 to rotate through the driving motor 6, accelerates the flow of gas through the cooling fan blade 20, thereby cooling and storing the cells below, and facilitating the growth and culture of the cells;
According to fig. 1 and 2, the operator moves the dust cover 9 and the slider 18 to one side of the driving motor 6, the rotating gear 19 below the driving motor 6 drives the rotating gear 19 on the same side to rotate, the rotating gear 19 drives the second lead screw 8 to rotate, the second lead screw 8 drives the dust cover 9 to move downwards, when the dust cover moves to a corresponding height, the two groups of rotating gears 19 are separated from each other, the dust cover 9 stops descending, the operator can separate cells right below the dust cover 9, and the mixture of substances in the air is avoided;
According to fig. 2, fig. 4 and fig. 5, then according to operating personnel's height, through holding rotatory piece 2, it rotates to drive awl tooth 14 through rotatory piece 2, it rotates to drive first lead screw 15 through awl tooth 14, it goes on vertical lift to drive interior threaded rod 4 through first lead screw 15, interior threaded rod 4 drives equipment box 5 and goes on vertical lift, operating personnel carries out cell separation operation in the below of dust cover 9, spliced pole 12 below will cultivate the disc 10 is taken out from the jack 13 of another group's cultivation disc 10 top below afterwards, and place cultivation disc 10 top with the cell after having separated, seal up the outside of cell through the sealing ring 11 in cultivation disc 10 outside, utilize refrigeration piece 16 to carry out cold-stored to the cell of separation simultaneously.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.
Claims (9)
1. The utility model provides a culture apparatus for cell separation is experimental, includes device base (1), equipment box (5) and cultivates disc (10), its characterized in that: the device is characterized in that the outer side of the device base (1) is connected with a rotating block (2), a supporting column (3) is connected directly above the device base (1), an inner threaded rod (4) is nested in the supporting column (3), a driving motor (6) is connected directly above the equipment box body (5), a sealing box door (7) is hinged and fixed on the outer side of the equipment box body (5), a second lead screw (8) is connected directly below the equipment box body (5), a dust cover (9) is connected directly below the second lead screw (8), a sealing ring (11) is connected directly below the cultivation disc (10), a connecting column (12) is connected directly below the sealing ring (11), a jack (13) is arranged on the upper surface of the cultivation disc (10), a conical tooth (14) is connected on the outer side of the rotating block (2), and a first lead screw (15) is connected on the outer side of the conical tooth (14), the equipment box is characterized in that a refrigerating piece (16) is arranged on the inner wall of the equipment box body (5), a rectangular sliding rail (17) is connected to the position right above the equipment box body (5), a sliding block (18) is connected to the position right below the rectangular sliding rail (17), a rotating gear (19) is connected to the position right below the sliding block (18), and cooling fan blades (20) are connected to the position right below the rotating gear (19).
2. The culture apparatus for cell isolation test according to claim 1, wherein: the diameter of support column (3) is less than the diameter of internal thread pole (4), and equipment box (5) constitute elevation structure through internal thread pole (4), rotary gear (19) and rotatory piece (2).
3. The culture apparatus for cell isolation test according to claim 1, wherein: the length of the second screw rod (8) is smaller than that of the dust cover (9), the dust cover (9) and the device base (1) are parallel to each other, and the device base (1) is of a hollow rectangular structure.
4. The culture apparatus for cell isolation test according to claim 1, wherein: the dust cover (9) and the second screw rod (8) form a rotating structure through a rectangular sliding rail (17), a sliding block (18) and rotating gears (19), and the number of the rotating gears (19) is 2.
5. The culture apparatus for cell isolation test according to claim 1, wherein: the cultivation disc (10) is bonded with the sealing ring (11) to form an integrated structure, and connecting columns (12) are distributed at the bottom of the sealing ring (11) at equal intervals.
6. The culture apparatus for cell isolation test according to claim 1, wherein: the sealing ring (11) is of an annular transparent structure, the diameter of the sealing ring (11) is smaller than that of the culture disc (10), and the 2 groups of culture discs (10) form a dismounting and mounting structure through the connecting column (12) and the insertion hole (13).
7. The culture apparatus for cell isolation test according to claim 1, wherein: the first screw rod (15) is perpendicular to the equipment box body (5), and the first screw rod (15) and the rotating block (2) form a rotating structure through a rotating gear (19).
8. The culture apparatus for cell isolation test according to claim 1, wherein: the sliding block (18) is connected with the first screw rod (15) through a bearing, the first screw rod (15) and the dust cover (9) form a sliding structure through the sliding block (18) and the rectangular sliding rail (17), and the length of the rectangular sliding rail (17) is larger than half of the length of the equipment box body (5).
9. the culture apparatus for cell isolation test according to claim 1, wherein: the cooling fan blades (20) and the refrigerating fins (16) are symmetrically distributed about the center line of the equipment box body (5), and the bottom of the equipment box body (5) is of an open structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910731916.7A CN110540931A (en) | 2019-08-06 | 2019-08-06 | Culture apparatus for cell separation test |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910731916.7A CN110540931A (en) | 2019-08-06 | 2019-08-06 | Culture apparatus for cell separation test |
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| Publication Number | Publication Date |
|---|---|
| CN110540931A true CN110540931A (en) | 2019-12-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910731916.7A Pending CN110540931A (en) | 2019-08-06 | 2019-08-06 | Culture apparatus for cell separation test |
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| CN (1) | CN110540931A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111790595A (en) * | 2020-07-15 | 2020-10-20 | 阴文慧 | Separation and purification device with biomaterial handles structure |
| CN112362801A (en) * | 2020-11-06 | 2021-02-12 | 四川清和科技有限公司 | Device for rapidly detecting algae removal effect |
| CN113930341A (en) * | 2021-11-16 | 2022-01-14 | 郑州大学第一附属医院 | Integrated esophageal cancer tumor cell culture and separation system |
-
2019
- 2019-08-06 CN CN201910731916.7A patent/CN110540931A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111790595A (en) * | 2020-07-15 | 2020-10-20 | 阴文慧 | Separation and purification device with biomaterial handles structure |
| CN111790595B (en) * | 2020-07-15 | 2022-05-06 | 临沂奥广源达生物科技有限公司 | Separation and purification device with biomaterial handles structure |
| CN112362801A (en) * | 2020-11-06 | 2021-02-12 | 四川清和科技有限公司 | Device for rapidly detecting algae removal effect |
| CN112362801B (en) * | 2020-11-06 | 2022-01-28 | 四川清和科技有限公司 | Device for rapidly detecting algae removal effect |
| CN113930341A (en) * | 2021-11-16 | 2022-01-14 | 郑州大学第一附属医院 | Integrated esophageal cancer tumor cell culture and separation system |
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Application publication date: 20191206 |