CN112167059A - Rhizoma bletillae tissue culture rooting culture device and method - Google Patents
Rhizoma bletillae tissue culture rooting culture device and method Download PDFInfo
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- CN112167059A CN112167059A CN202011060693.5A CN202011060693A CN112167059A CN 112167059 A CN112167059 A CN 112167059A CN 202011060693 A CN202011060693 A CN 202011060693A CN 112167059 A CN112167059 A CN 112167059A
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- culture dish
- bletilla striata
- growth
- rooting
- transparent cover
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The rooting cultivation device for bletilla striata tissue culture comprises a base, wherein a transparent cover is arranged on the surface of the base, an operating mechanism penetrates through the center of the transparent cover in a sliding mode, the bottom end of the operating mechanism is connected with growth brackets distributed in an annular array mode, the growth brackets are used for bearing bletilla striata tissues, and the operating mechanism is used for driving the growth brackets to vertically move and rotate; sterile seed culture dishes, induction culture dishes and rooting culture dishes which are distributed in an annular array are embedded in the base at intervals; the outer wall of the transparent cover is hinged with a sealing cover plate, and the top surface inside the transparent cover is provided with a plurality of illuminating lamps and cameras; the invention can realize the cyclic transfer of the growth bracket among the strain-free culture dish, the induction culture dish and the rooting culture dish by utilizing the operating mechanism, so that the whole rooting culture process is carried out in the transparent cover, the growth process is in a constant environment, the growth effect is effectively ensured, only one constant environment needs to be set, the energy consumption is saved, and the operation is simple and convenient.
Description
Technical Field
The invention belongs to the technical field of bletilla striata tissue culture, and particularly relates to a rooting culture device and method for bletilla striata tissue culture.
Background
The surface of the common bletilla pseudobulb is grey white or yellow white, a plurality of circles of concentric links and brown dotted fibrous root marks are formed, a protruded stem mark is formed on the common bletilla pseudobulb, and a mark for connecting another tuber is formed below the common bletilla pseudobulb; hard, difficult to break, the cross section is white like; has astringent, hemostatic, repercussive, and granulation promoting effects;
in the research process of bletilla striata, bletilla striata tissues need to be cultured, in the research process, long-term observation and multiple transfer culture of bletilla striata are needed, the existing culture process is troublesome, the bletilla striata tissues are easily damaged in the transfer process, and the growth environment of the bletilla striata tissues is also frequently changed.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a rhizoma bletillae tissue culture rooting culture device, which has the following specific technical scheme:
the rooting cultivation device for bletilla striata tissue culture comprises a base, wherein a transparent cover is arranged on the surface of the base, an operating mechanism penetrates through the center of the transparent cover in a sliding mode, the bottom end of the operating mechanism is connected with growth brackets distributed in an annular array mode, the growth brackets are used for bearing bletilla striata tissues, and the operating mechanism is used for driving the growth brackets to vertically move and rotate;
sterile seed culture dishes, induction culture dishes and rooting culture dishes which are distributed in an annular array are embedded in the base at intervals; the top parts of the sterile seed culture dish, the induction culture dish and the rooting culture dish are respectively and oppositely provided with a growth bracket;
the outer wall of the transparent cover is hinged with a sealing cover plate, the top surface inside the transparent cover is provided with a plurality of illuminating lamps and cameras, and the tops of the sterile seed culture dish, the induction culture dish and the rooting culture dish are all provided with a camera oppositely; and the outer side of the transparent cover is communicated with a moisture supply mechanism and a hot air supply mechanism.
Further, the growth bracket comprises a pipe body and a support cover, wherein the support cover is vertically arranged at the bottom end of the pipe body, the support cover is of a mesh structure, and the pipe body and the support cover are positioned at the same center line; and scale strips are distributed on the outer wall of the pipe body.
Furthermore, the growth bracket also comprises identification rods, wherein the identification rods are vertically and symmetrically arranged on two sides of the tube body, the distance between one identification rod and the support cover is 1.8-2.2 cm, and the distance between the other identification rod and the support cover is 3-5 cm.
Furthermore, the operating mechanism comprises a main pipe body, an operating plate and fixing rods, wherein the operating plate is arranged at the top end of the main pipe body and is stopped on the top surface of the transparent cover, the fixing rods distributed in an annular array are arranged at the bottom end of the main pipe body, and the end parts of the fixing rods are assembled and connected with the growth bracket.
Furthermore, the bottom end of the fixing rod is provided with a vertical downward stud, and the stud is screwed into the pipe body.
Furthermore, the top end and the bottom end of the main pipe body are both of an open structure, the operating mechanism further comprises a liquid guiding component, the liquid guiding component further comprises a support column and a liquid discharging pipe, the support column is vertically arranged at the center of the surface of the base, the top end of the support column is rotatably embedded in the main pipe body, the side wall of the support column is communicated with the liquid discharging pipe which is inclined downwards, the liquid discharging pipe extends downwards to the induction culture dish, and the main pipe body, the support column and the liquid discharging pipe form a continuous liquid feeding channel; an axial movable gap is reserved between the support and the main pipe body.
The rooting cultivation method for the tissue culture of the bletilla striata comprises the following steps:
s1, rooting cultivation:
rotating the operating mechanism to transfer and immerse the growth bracket with the green bud points into an induction culture dish, transfer and immerse the growth bracket with the bletilla striata seedlings into a rooting culture dish, and transfer and immerse the growth bracket with the bletilla striata seeds into an aseptic seeding culture dish;
sealing culture, wherein the moisture supply mechanism supplies moisture into the transparent cover, the hot air supply mechanism introduces hot air into the transparent cover, and the illuminating lamp provides illumination for enough time;
regularly observing growth states of bletilla striata in the sterile seeding culture dish, the induction culture dish and the rooting culture dish every day, and recording the height of the tissue according to the scales on the tube body;
culturing for 3-5 days, primarily converting green bud spots in the induction culture dish into bletilla striata sprouts, and adding quantitative auxin into the induction culture dish;
after culturing for 20-60 days, the seeds in the sterile seeding culture dish germinate spherical green bud points; inducing the green bud points in the culture dish to root to form bletilla striata sprouts, wherein the height of the bletilla striata sprouts is 1.8cm-2.2 cm; the bletilla striata bud seedlings in the rooting culture dish grow to 3-5cm to form bletilla striata tissue plantlets; proceeding to S2;
s2, taking out bletilla striata tissue plantlets:
opening the cover plate, lifting up and rotating the operating mechanism, and detaching the growth bracket with the bletilla striata tissue plantlets;
taking down bletilla striata tissue plantlets, spreading bletilla striata seeds in an empty growth bracket,
mounting a growth bracket and covering a cover plate;
returning to S1.
Further, the observation of the growth states of the bletilla striata in the sterile seeding culture dish, the induction culture dish and the rooting culture dish is as follows: the observation method has two methods, one is to directly observe the growth state of the rhizoma bletillae tissues through the transparent cover; the other is to observe the growth state of the bletilla striata tissues in the sterile seeding culture dish and the induction culture dish through a camera and a display screen.
Further, the adding of the quantitative auxin to the induction culture dish is specifically as follows: the auxin is discharged to the induction culture dish through a liquid feeding channel formed by the main pipe body, the support and the liquid discharging pipe.
The invention has the beneficial effects that: utilize operating device to realize that the growth bracket circulates the switching between no bacterial culture dish, induction culture dish and rooting culture dish for whole rooting culture process all goes on in the translucent cover, and the growth process all is in under the invariable environment, the effectual growth effect of having guaranteed, only need set for a invariable environment can, practice thrift the energy consumption, it is easy and simple to handle.
Drawings
FIG. 1 is a schematic structural diagram of a rhizoma bletillae rooting culture device for tissue culture according to the present invention;
FIG. 2 is a schematic view showing the overall structure of the transparent cover of the present invention;
FIG. 3 shows a schematic top view of the base of the present invention;
FIG. 4 shows a schematic view of the growth carriage structure of the present invention;
FIG. 5 shows a schematic view of the operating mechanism of the present invention;
shown in the figure: 1. a base; 2. an aseptic seeding culture dish; 3. a growth bracket; 31. a pipe body; 32. an identification rod; 33. supporting the cover; 4. an induction culture dish; 5. a rooting culture dish; 6. an operating mechanism; 61. a primary tube; 62. an operation panel; 63. fixing the rod; 631. a stud; 64. a pillar; 65. a liquid discharge pipe; 7. a transparent cover; 71. a cover plate; 8. an illuminating lamp; 9. a camera; 10. a moisture supply mechanism; 11. and a hot air supply mechanism.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
As shown in fig. 1 to 3, a rooting culture device for tissue culture of bletilla striata,
the device comprises a base 1, wherein a transparent cover 7 is arranged on the surface of the base 1, an operating mechanism 6 penetrates through the center of the transparent cover 7 in a sliding manner, the bottom end of the operating mechanism 6 is connected with growth brackets 3 distributed in an annular array manner, the growth brackets 3 are used for bearing rhizoma bletillae tissues, and the operating mechanism 6 is used for driving the growth brackets 3 to vertically move and rotate; sterile seed culture dishes 2, induction culture dishes 4 and rooting culture dishes 5 which are distributed in an annular array are embedded in the base 1 at intervals; the top parts of the sterile seed culture dish 2, the induction culture dish 4 and the rooting culture dish 5 are respectively and oppositely provided with a growth bracket 3;
the growth bracket can bear bletilla striata tissues, integrated rapid transfer can be realized, the mesh structure can enable culture liquid to be immersed into the growth bracket, and normal culture of the bletilla striata tissues is guaranteed; the sterile seed culture dish is used for sterile culture of seeds to generate green bud spots, and the culture solution in the sterile culture medium is as follows: MS +6-BA0.05mg/L + NAA0.1-0.5mg/L, wherein contains sucrose 3%, agar powder 0.7%, and PH 5.8; the induction culture dish is used for culturing the bud to root, and culture solution in the induction culture dish is as follows: MS +6-BA0.1mg/L, wherein the content of sucrose is 3%, agar powder is 0.7%, and PH is 5.8; when the induction culture dish is cultured in the later stage, auxin is added; the culture solution in the rooting culture dish is as follows: MS + NAA0.1-0.5mg/L, which contains cane sugar 2%, agar powder 0.7%, and PH 5.8;
the operating mechanism can realize the cyclic transfer of the growth bracket among the strain-free culture dish, the induction culture dish and the rooting culture dish, so that the whole rooting culture process is carried out in the transparent cover, the growth process is in a constant environment, the growth effect is effectively ensured, only one constant environment needs to be set, the energy consumption is saved, and the operation is simple and convenient;
the outer wall of the transparent cover 7 is hinged with a sealing cover plate 71, and the sealing cover plate 71 is used for facilitating an operator to open the transparent cover 7 for loading and unloading operations; the transparent cover is used for facilitating an operator to observe the growth state;
the top surface of the interior of the transparent cover 7 is provided with a plurality of illuminating lamps 8 and cameras 9, and the tops of the sterile seed culture dish 2, the induction culture dish 4 and the rooting culture dish 5 are all provided with one camera 9 oppositely; the outer side of the transparent cover 7 is communicated with a moisture supply mechanism 10 and a hot air supply mechanism 11; the camera 9 can carefully observe the growth state of the bletilla striata seeds so as to transfer the bletilla striata seeds to another culture dish in a proper growth state; the moisture supply mechanism is exemplified by a humidifier, and the hot air supply mechanism 11 is exemplified by a hot air blower, and provides hot air and oxygen for uniform heating.
As shown in fig. 4, the growth bracket 3 includes a tube 31 and a support cover 33, the support cover 33 is vertically disposed at the bottom end of the tube 31, the support cover 33 is a mesh structure, and the tube 31 and the support cover 33 are located at the same center line; scale strips are distributed on the outer wall of the tube body 31; the tube body is used as a positioning part, the support cover is used for bearing bletilla striata tissues, and the mesh structure is used for enabling the bletilla striata tissues to be fully immersed into the culture solution; the roots of the bletilla striata tissues can grasp all meshes, and the support cover can play a role in positioning the roots and ensure the stable growth of the bletilla striata tissues; the scale bar can make things convenient for operating personnel to observe the bletilla and organize and get overall height, the record of being convenient for.
The growth bracket 3 further comprises mark rods 32, the two sides of the tube body 31 are vertically and symmetrically provided with the mark rods 32, the distance between one mark rod 32 and the support cover 33 is 1.8cm-2.2cm, and the distance between the other mark rod and the support cover is 3-5 cm; the marking rod can be used as an operating part for installing the pipe body, so that the pipe body is conveniently connected with the operating mechanism; one marking rod is used as the growth marking height of the induction culture dish, and the other marking rod is used as the growth marking height of the rooting culture dish, so that an operator can judge the growth height of the bletilla striata tissue more visually.
As shown in fig. 5, the operating mechanism 6 includes a main pipe 61, an operating plate 62 and fixing rods 63, the operating plate 62 is disposed at the top end of the main pipe 61, the operating plate 62 is stopped on the top surface of the transparent cover 7, the fixing rods 63 distributed in an annular array are disposed at the bottom end of the main pipe 61, and the ends of the fixing rods 63 are assembled and connected with the growth bracket 3; the operation plate can be used as a stop positioning structure, and the fixing rod can position each growth bracket 3;
a vertical downward stud 631 is arranged at the bottom end of the fixed rod 63, and the stud 631 is screwed into the pipe body 31; the double-screw bolt can with body rapid Assembly, and the dismantlement is connected more portably.
The top end and the bottom end of the main pipe body 61 are both of an open structure, the operating mechanism 6 further comprises a liquid guiding component, the liquid guiding component further comprises a support column 64 and a liquid discharging pipe 65, the support column 64 is vertically arranged at the center of the surface of the base 1, the top end of the support column 64 is rotatably embedded in the main pipe body 61, the side wall of the support column 64 is communicated with the liquid discharging pipe 65 which inclines downwards, the liquid discharging pipe 65 extends downwards to the induction culture dish 4, and the main pipe body 61, the support column 64 and the liquid discharging pipe 65 form a continuous liquid feeding channel; an axial movable gap is reserved between the strut 64 and the main pipe body 61; the pillar is rotationally connected with the main pipe body to ensure that the rotation of the main pipe body is not influenced while the liquid adding channel is formed, and the vertical movement of the main pipe body can not be influenced by the arrangement of the axial movable gap, so that the liquid adding channel can normally circulate while the main pipe body can normally lift and rotate; the support and the drain pipe are kept still, and the drain pipe can supplement auxin to the induction culture dish; be responsible for opening on the body and when not adding auxin, can cover the rubber buffer and seal, when adding liquid, cover the rubber buffer.
A temperature and humidity sensor is arranged in the transparent cover.
The rooting cultivation method for the tissue culture of the bletilla striata comprises the following steps:
s1, rooting cultivation:
rotating the operating mechanism 6 to transfer and immerse the growth bracket with the green bud points into the induction culture dish 4, transfer and immerse the growth bracket with the bletilla striata seedlings into the rooting culture dish 5, and transfer and immerse the growth bracket with the bletilla striata seeds into the sterile seeding culture dish 2; the step can adjust the position of each growth bracket, so that each bletilla striata tissue can enter a designated culture dish; the specific operation is as follows: the main pipe body 61 is rotated, and then the fixing rods 63 drive the growth brackets 3 to rotate;
sealing culture, wherein the moisture supply mechanism 10 supplies moisture into the transparent cover 7, the hot air supply mechanism 11 introduces hot air into the transparent cover 7, and the illuminating lamp 8 provides illumination for a sufficient time;
regularly observing the growth states of the bletilla striata in the sterile seeding culture dish 2, the induction culture dish 4 and the rooting culture dish 5 every day, and recording the tissue height according to the scales on the tube body 31;
the observation methods are two, one is to directly observe the growth state of the rhizoma bletillae tissues through the transparent cover 9; the other is that the growth states of the bletilla striata tissues in the sterile seeding culture dish 2, the induction culture dish 4 and the rooting culture dish 5 are observed through a camera 9 and a display screen; the observation image shot by the camera can be transmitted to an external display screen, so that operators can see the growth conditions of rhizoma bletillae more clearly;
after culturing for 3-5 days, primarily converting green bud spots in the induction culture dish 4 into bletilla striata sprouts, and adding quantitative auxin into the induction culture dish 4; the newly formed bletilla striata sprouts are usually weak and mostly have no roots, so that the concentration of auxin can be increased, the seedlings are promoted to root, and the robustness of the seedlings is improved; the auxin is discharged to the induction culture dish 4 through a feeding channel formed by the main tube 61, the support 64 and the drain tube 65.
After culturing for 20-60 days, the seeds in the sterile seeding culture dish 2 germinate spherical green bud points; inducing the green bud points in the culture dish 4 to root to form bletilla striata sprouts, wherein the height of the bletilla striata sprouts is 2 cm; the bletilla striata sprouts in the rooting culture dish 5 grow to 4cm to form bletilla striata tissue plantlets; proceeding to S2; bletilla striata bud seedlings are aligned to the marking rods, and bletilla striata tissue seedlings are aligned to the other marking rod;
the whole process is carried out in a transparent cover, and the growth environment is kept unchanged;
s2, taking out bletilla striata tissue plantlets:
opening the cover plate 71, lifting up and rotating the operating mechanism 6, and detaching the growth bracket with the bletilla striata tissue plantlets;
taking down bletilla striata tissue plantlets, spreading bletilla striata seeds in an empty growth bracket,
mounting a growth bracket and covering a cover plate 71; mounting a growth bracket, namely screwing the pipe body 31 and the stud 631 together;
returning to S1.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (9)
1. Bletilla is root-growing culture device for tissue culture, its characterized in that: the bletilla striata tissue culture device comprises a base, wherein a transparent cover is arranged on the surface of the base, an operating mechanism penetrates through the center of the transparent cover in a sliding mode, the bottom end of the operating mechanism is connected with growth brackets distributed in an annular array mode, the growth brackets are used for bearing bletilla striata tissues, and the operating mechanism is used for driving the growth brackets to vertically move and rotate;
sterile seed culture dishes, induction culture dishes and rooting culture dishes which are distributed in an annular array are embedded in the base at intervals; the top parts of the sterile seed culture dish, the induction culture dish and the rooting culture dish are respectively and oppositely provided with a growth bracket;
the outer wall of the transparent cover is hinged with a sealing cover plate, the top surface inside the transparent cover is provided with a plurality of illuminating lamps and cameras, and the tops of the sterile seed culture dish, the induction culture dish and the rooting culture dish are all provided with a camera oppositely; and the outer side of the transparent cover is communicated with a moisture supply mechanism and a hot air supply mechanism.
2. The rooting cultivation device for bletilla striata tissue culture according to claim 1, wherein: the growth bracket comprises a pipe body and a support cover, wherein the support cover is vertically arranged at the bottom end of the pipe body, the support cover is of a mesh structure, and the pipe body and the support cover are positioned at the same center line; and scale strips are distributed on the outer wall of the pipe body.
3. The rooting cultivation device for bletilla striata tissue culture according to claim 2, wherein: the growth bracket further comprises identification rods, the two sides of the tube body are vertically and symmetrically provided with the identification rods, the distance between one identification rod and the support cover is 1.8cm-2.2cm, and the distance between the other identification rod and the support cover is 3-5 cm.
4. The rooting cultivation device for bletilla striata tissue culture according to claim 2, wherein: the operating mechanism comprises a main pipe body, an operating plate and fixing rods, wherein the operating plate is arranged at the top end of the main pipe body and is stopped on the top surface of the transparent cover, the fixing rods distributed in an annular array mode are arranged at the bottom end of the main pipe body, and the end portions of the fixing rods are assembled and connected with the growth bracket.
5. The rooting cultivation device for bletilla striata tissue culture according to claim 4, wherein: the bottom of dead lever is equipped with vertical decurrent double-screw bolt, the double-screw bolt screw in is to the body.
6. The rooting cultivation device for bletilla striata tissue culture according to claim 5, wherein: the top end and the bottom end of the main pipe body are both of an open structure, the operating mechanism further comprises a liquid guiding component, the liquid guiding component further comprises a support and a liquid discharging pipe, the support is vertically arranged at the center of the surface of the base, the top end of the support is rotatably embedded in the main pipe body, the side wall of the support is communicated with the liquid discharging pipe which is inclined downwards, the liquid discharging pipe extends downwards to the induction culture dish, and the main pipe body, the support and the liquid discharging pipe form a continuous liquid feeding channel; an axial movable gap is reserved between the support and the main pipe body.
7. The rooting cultivation method for bletilla striata tissue culture is characterized by comprising the following steps: the rooting cultivation device according to claim 1, wherein the cultivation method comprises the following steps:
s1, rooting cultivation:
rotating the operating mechanism to transfer and immerse the growth bracket with the green bud points into an induction culture dish, transfer and immerse the growth bracket with the bletilla striata seedlings into a rooting culture dish, and transfer and immerse the growth bracket with the bletilla striata seeds into an aseptic seeding culture dish;
sealing culture, wherein the moisture supply mechanism supplies moisture into the transparent cover, the hot air supply mechanism introduces hot air into the transparent cover, and the illuminating lamp provides illumination for enough time;
regularly observing growth states of bletilla striata in the sterile seeding culture dish, the induction culture dish and the rooting culture dish every day, and recording the height of the tissue according to the scales on the tube body;
culturing for 3-5 days, primarily converting green bud spots in the induction culture dish into bletilla striata sprouts, and adding quantitative auxin into the induction culture dish;
after culturing for 20-60 days, the seeds in the sterile seeding culture dish germinate spherical green bud points; inducing the green bud points in the culture dish to root to form bletilla striata sprouts, wherein the height of the bletilla striata sprouts is 1.8cm-2.2 cm; the bletilla striata bud seedlings in the rooting culture dish grow to 3-5cm to form bletilla striata tissue plantlets; proceeding to S2;
s2, taking out bletilla striata tissue plantlets:
opening the cover plate, lifting up and rotating the operating mechanism, and detaching the growth bracket with the bletilla striata tissue plantlets;
taking down bletilla striata tissue plantlets, spreading bletilla striata seeds in an empty growth bracket,
mounting a growth bracket and covering a cover plate;
returning to S1.
8. The rooting cultivation method for bletilla striata tissue culture according to claim 7, characterized in that: the observation sterile seeding culture dish, the induction culture dish and the rooting culture dish are specifically as follows: the observation method has two methods, one is to directly observe the growth state of the rhizoma bletillae tissues through the transparent cover; the other method is that the growth state of the bletilla striata tissues is observed through a camera, and then images are shot and transmitted to a display screen.
9. The rooting cultivation method for bletilla striata tissue culture according to claim 7, characterized in that: the addition of quantitative auxin to the induction culture dish is specifically: the auxin is discharged to the induction culture dish through a liquid feeding channel formed by the main pipe body, the support and the liquid discharging pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202011060693.5A CN112167059A (en) | 2020-09-30 | 2020-09-30 | Rhizoma bletillae tissue culture rooting culture device and method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011060693.5A CN112167059A (en) | 2020-09-30 | 2020-09-30 | Rhizoma bletillae tissue culture rooting culture device and method |
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| CN112167059A true CN112167059A (en) | 2021-01-05 |
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| CN202011060693.5A Withdrawn CN112167059A (en) | 2020-09-30 | 2020-09-30 | Rhizoma bletillae tissue culture rooting culture device and method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115067211A (en) * | 2022-06-24 | 2022-09-20 | 唐山师范学院 | Multifunctional temperature control device for microspore culture |
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2020
- 2020-09-30 CN CN202011060693.5A patent/CN112167059A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115067211A (en) * | 2022-06-24 | 2022-09-20 | 唐山师范学院 | Multifunctional temperature control device for microspore culture |
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Application publication date: 20210105 |