CN212800392U - Tumor cell culture device - Google Patents
Tumor cell culture device Download PDFInfo
- Publication number
- CN212800392U CN212800392U CN202021125634.7U CN202021125634U CN212800392U CN 212800392 U CN212800392 U CN 212800392U CN 202021125634 U CN202021125634 U CN 202021125634U CN 212800392 U CN212800392 U CN 212800392U
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- China
- Prior art keywords
- fixedly connected
- incubator
- motor
- lead screw
- cell culture
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- 238000004113 cell culture Methods 0.000 title claims abstract description 26
- 210000004881 tumor cell Anatomy 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000002699 waste material Substances 0.000 claims description 14
- 238000005086 pumping Methods 0.000 claims description 4
- 238000005516 engineering process Methods 0.000 abstract description 6
- 206010028980 Neoplasm Diseases 0.000 abstract description 5
- 208000035143 Bacterial infection Diseases 0.000 abstract description 3
- 208000022362 bacterial infectious disease Diseases 0.000 abstract description 3
- 235000015097 nutrients Nutrition 0.000 description 11
- 210000004027 cell Anatomy 0.000 description 9
- 230000001464 adherent effect Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a tumor cell culture apparatus relates to tumour science and technology art field, including the incubator, the right side fixedly connected with motor cabinet of incubator, the last fixed surface of motor cabinet is connected with first motor, the output pivot fixedly connected with lead screw of first motor, the lead screw extends to the inside of incubator, the surface engagement of lead screw is connected with screw nut, screw nut's side fixedly connected with water pump, the liquid suction pipe has been cup jointed to the bottom surface of water pump, the fixed intercommunication of output of water pump has flexible pipe, screw nut's bottom surface fixedly connected with connecting rod, the bottom surface fixedly connected with connecting plate of connecting rod, the last fixed surface of connecting plate is connected with the second motor, the output pivot fixedly connected with axis of rotation of second motor, the one end fixedly connected with gear of second motor is kept away from to the axis of rotation. This tumour cell culture apparatus possesses and changes the culture solution convenience, reduces the bacterial infection and accelerates the advantage of cultivation progress, sets up the blotter and makes the culture dish keep stable.
Description
Technical Field
The utility model relates to a tumour science and technology field specifically is a tumour cell culture apparatus.
Background
Cell culture refers to a method for simulating in-vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro to enable the in-vivo environment to survive, grow, reproduce and maintain main structure and functions, the cell culture is also called cell cloning technology, the formal term in biology is cell culture technology, the cell culture is an essential process for the whole bioengineering technology or one of the biological cloning technologies, the cell culture is the large-scale cloning of cells, tumors are divided into benign tumors and malignant tumors in the medical field according to the cell characteristics of new organisms and the degree of harm to organisms, and the cell culture is one of the means for treating tumors in oncology.
At present, be used for some devices of cell culture of oncology in the medical treatment when changing the culture solution, need open culture apparatus and extract the culture solution, because open culture apparatus and make the bacterium get into culture apparatus easily, probably lead to the cultured cell to infect the death, influence the cell culture precision, easily cause the adherent cell to drop when removing culture apparatus moreover, be extracted, lead to the cultivation progress to slow down.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a tumor cell culture apparatus, it is convenient to possess the change culture solution, reduce the bacterial infection and accelerate the advantage of cultivateing the progress, it need open culture apparatus and extract the culture solution to have solved some current cell culture apparatus when changing the culture solution, owing to open culture apparatus and make the bacterium get into culture apparatus easily, probably lead to cultivateing the cell infection death, influence the cell culture precision, and easily cause the adherent cell to drop when removing culture apparatus in addition, be extracted, lead to the problem that the cultivation progress slows down.
The utility model discloses a solve above-mentioned technical problem, provide following technical scheme: the utility model provides a tumor cell culture apparatus, includes the incubator, the right side fixedly connected with motor cabinet of incubator, the first motor of last fixed surface of motor cabinet is connected with, the output pivot fixedly connected with lead screw of first motor, the lead screw extends to the inside of incubator, the surface engagement of lead screw is connected with screw nut, screw nut's side fixedly connected with water pump, the liquid suction pipe has been cup jointed to the bottom surface of water pump, the fixed intercommunication of output of water pump has flexible pipe, screw nut's bottom surface fixedly connected with connecting rod, the bottom surface fixedly connected with connecting plate of connecting rod, the last fixed surface of connecting plate is connected with the second motor, the output pivot fixedly connected with axis of rotation of second motor, the one end fixedly connected with gear of second motor is kept away from in the axis of rotation.
Furthermore, a spur rack is fixedly connected to the side face of the liquid pumping pipe, and the spur rack is in meshed connection with the gear.
Through adopting above-mentioned technical scheme, set up spur rack and gear engagement and connect and guarantee that the liquid suction pipe can rise or descend and dip in the nutrient solution in the culture dish, make the liquid suction pipe absorb the nutrient solution smoothly.
Furthermore, a limiting groove is formed in the inner top wall of the incubator, a limiting block is fixedly connected to the upper surface of the lead screw nut, and the limiting block is connected with the limiting groove in a sliding mode.
Through adopting above-mentioned technical scheme, set up the spacing groove and carry on spacingly to the stopper, when making lead screw nut remove, do not take place the skew, make the connecting plate remain stable.
Further, the left side fixedly connected with fixed plate of incubator, the upper surface sliding connection of fixed plate has the waste liquid bucket, the flexible end of flexible pipe runs through the incubator and extends to the inside of waste liquid bucket.
Through adopting above-mentioned technical scheme, set up the waste liquid bucket and collect the waste liquid of taking out, convenient operation uses manpower sparingly.
Further, the inner bottom wall fixedly connected with fixing base of incubator, the last fixed surface of fixing base is connected with the blotter, the last fixed surface of blotter is connected with the culture dish.
Through adopting above-mentioned technical scheme, set up the fixing base and support and fixed culture dish, receive the shaking force when setting up the blotter and reducing the incubator and remove, make the culture dish remain stable, prevent that the adherent cell from droing.
Furthermore, the fixed surface of incubator inlays and has the observation window, the front of incubator articulates there is the door that opens and shuts.
Through adopting above-mentioned technical scheme, set up the observation window and conveniently observe the condition in the incubator, set up the door that opens and shuts and conveniently operate in the incubator.
Further, the bottom fixedly connected with of flexible end of flexible pipe is sealed to fill up, the inside wall of incubator is fixed to be inlayed and is had the bearing, the one end that first motor was kept away from to the lead screw is fixedly connected with the inner circle of bearing.
Through adopting above-mentioned technical scheme, set up sealed the pad and make flexible pipe have good leakproofness, set up the bearing and reduce the frictional force between lead screw and the incubator, reduce the loss of mechanical energy, the life of extension equipment.
Compared with the prior art, the tumor cell culture device has the following beneficial effects:
1. the utility model discloses it is convenient to possess the change culture solution, reduce the bacterial infection and accelerate the advantage of cultivateing the progress, connect through setting up spur rack and gear engagement and guarantee that the liquid suction pipe can rise or descend and dip in the nutrient solution in the culture dish, make the liquid suction pipe absorb the nutrient solution smoothly, set up the spacing groove and carry on spacingly to the stopper, when making lead screw nut remove, do not take place the skew, make the connecting plate remain stable, set up the waste liquid bucket and collect the waste liquid of taking out, convenient operation uses manpower sparingly.
2. The utility model discloses set up the fixing base and support and fixed culture dish, receive the shaking force when setting up the blotter and reducing the incubator and remove, make the culture dish remain stable, prevent that the adherent cell from droing, set up the observation window and come the condition in conveniently observing the culture box, set up the door that opens and shuts and come the convenience and operate in the culture box, it has good leakproofness to set up sealed the pad and make flexible pipe, set up the bearing and reduce the frictional force between lead screw and the culture box, reduce the loss of mechanical energy, the life of extension equipment.
Drawings
Fig. 1 is a sectional view of the present invention;
FIG. 2 is a general schematic view of the present invention;
FIG. 3 is a perspective view of the liquid suction tube of the present invention;
fig. 4 is a sectional view of the liquid suction tube of the present invention.
In the figure: 1-incubator, 2-motor base, 3-first motor, 4-screw rod, 5-spacing groove, 6-spacing block, 7-observation window, 8-culture dish, 9-buffer pad, 10-fixing base, 11-liquid suction pipe, 12-fixing plate, 13-waste liquid barrel, 14-telescopic pipe, 15-bearing, 16-opening and closing door, 17-connecting plate, 18-screw rod nut, 19-water pump, 20-rotating shaft, 21-second motor, 22-gear, 23-straight rack, 24-connecting rod and 25-sealing gasket.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution: a tumor cell culture device comprises an incubator 1, a motor base 2 is fixedly connected to the right side of the incubator 1, a first motor 3 is fixedly connected to the upper surface of the motor base 2, a lead screw 4 is fixedly connected to an output rotating shaft of the first motor 3, the lead screw 4 extends into the incubator 1, a lead screw nut 18 is connected to the outer surface of the lead screw 4 in a meshed manner, a water pump 19 is fixedly connected to the side surface of the lead screw nut 18, a liquid pumping pipe 11 is sleeved on the bottom surface of the water pump 19, an extension pipe 14 is fixedly communicated with the output end of the water pump 19, a connecting rod 24 is fixedly connected to the bottom surface of the lead screw nut 18, a connecting plate 17 is fixedly connected to the bottom surface of the connecting rod 24, a second motor 21 is fixedly connected to the upper surface of the connecting plate 17, a rotating shaft 20 is fixedly connected to the output rotating shaft of the second motor 21, a gear 22, spur rack 23 and gear 22 meshing are connected, it connects to set up spur rack 23 and gear 22 meshing and guarantees that liquid suction pipe 11 can rise or descend and dip in the nutrient solution in culture dish 8, make liquid suction pipe 11 absorb the nutrient solution smoothly, spacing groove 5 has been seted up to the interior roof of incubator 1, screw nut 18's last fixed surface is connected with stopper 6, stopper 6 and 5 sliding connection in spacing groove, it is spacing to set up spacing groove 5 to carry out stopper 6, when making screw nut 18 remove, the skew does not take place, make connecting plate 17 remain stable.
The left side of the incubator 1 is fixedly connected with a fixed plate 12, the upper surface of the fixed plate 12 is connected with a waste liquid barrel 13 in a sliding way, the telescopic end of a telescopic pipe 14 penetrates through the incubator 1 and extends to the inside of the waste liquid barrel 13, the waste liquid barrel 13 is arranged to collect extracted waste liquid, the operation is convenient and labor-saving, the inner bottom wall of the incubator 1 is fixedly connected with a fixed seat 10, the upper surface of the fixed seat 10 is fixedly connected with a cushion pad 9, the upper surface of the cushion pad 9 is fixedly connected with a culture dish 8, the fixed seat 10 is arranged to support and fix the culture dish 8, the cushion pad 9 is arranged to reduce the vibration force applied to the incubator 1 during movement, so that the culture dish 8 is kept stable and prevents adherent cells from falling off, the upper surface of the incubator 1 is fixedly inlaid with an observation window 7, the front surface of the incubator 1 is hinged with an opening and closing door 16, the observation window 7 is arranged to facilitate the, the bottom fixedly connected with of the flexible end of flexible pipe 14 seals up 25, and the inside wall of incubator 1 is fixed to be inlayed and is had bearing 15, and the one end that first motor 3 was kept away from to lead screw 4 and the inner circle fixed connection of bearing 15 set up sealed 25 and make flexible pipe 14 have good leakproofness, set up bearing 15 and reduce the frictional force between lead screw 4 and the incubator 1, reduce the loss of mechanical energy, the life of extension equipment.
The utility model discloses well first motor 3 and gear 22 are the wide application that has disclosed and the known technology of daily life, and first motor 3 and gear 22 are a servo motor, have light in weight, small advantage.
During the use, when the culture solution in the culture dish 8 needs to be changed, start second motor 21, second motor 21 drives gear 22 through axis of rotation 20 and rotates, gear 22 drives spur rack 23 and descends, go deep into the nutrient solution, open water pump 19 and first motor 3 this moment, the nutrient solution in culture dish 8 is taken out to water pump 19, first motor 3 rotates through lead screw 4, it moves on lead screw 4 to drive lead screw nut 18, lead screw nut 18 drives connecting rod 24 and connecting plate 17 and makes liquid suction pipe 11 uniform velocity move, even absorption nutrient solution, the nutrient solution flows into in waste liquid bucket 13 after taking out, this process can not remove culture dish 8 and avoid, the droing of adherent cell, after the extraction, add new nutrient solution in culture dish 8 can.
Claims (7)
1. A tumor cell culture device, includes incubator (1), its characterized in that: the right side of the incubator (1) is fixedly connected with a motor base (2), the upper surface of the motor base (2) is fixedly connected with a first motor (3), an output rotating shaft of the first motor (3) is fixedly connected with a lead screw (4), the lead screw (4) extends to the inside of the incubator (1), the outer surface of the lead screw (4) is meshed with a lead screw nut (18), the side surface of the lead screw nut (18) is fixedly connected with a water pump (19), the bottom surface of the water pump (19) is sleeved with a liquid pumping pipe (11), the output end of the water pump (19) is fixedly communicated with a telescopic pipe (14), the bottom surface of the lead screw nut (18) is fixedly connected with a connecting rod (24), the bottom surface of the connecting rod (24) is fixedly connected with a connecting plate (17), the upper surface of the connecting plate (17) is fixedly connected with a second motor (21), the output rotating shaft of the second motor (21), one end of the rotating shaft (20) far away from the second motor (21) is fixedly connected with a gear (22).
2. A tumor cell culture apparatus according to claim 1, wherein: the side face of the liquid pumping pipe (11) is fixedly connected with a spur rack (23), and the spur rack (23) is in meshed connection with a gear (22).
3. A tumor cell culture apparatus according to claim 1, wherein: spacing groove (5) have been seted up to the interior roof of incubator (1), the last fixed surface of screw nut (18) is connected with stopper (6), stopper (6) and spacing groove (5) sliding connection.
4. A tumor cell culture apparatus according to claim 1, wherein: the left side fixedly connected with fixed plate (12) of incubator (1), the upper surface sliding connection of fixed plate (12) has waste liquid bucket (13), the flexible end of flexible pipe (14) runs through incubator (1) and extends to the inside of waste liquid bucket (13).
5. A tumor cell culture apparatus according to claim 1, wherein: the inner bottom wall fixedly connected with fixing base (10) of incubator (1), the last fixed surface of fixing base (10) is connected with blotter (9), the last fixed surface of blotter (9) is connected with culture dish (8).
6. A tumor cell culture apparatus according to claim 1, wherein: the upper surface of incubator (1) is fixed and is inlayed there is observation window (7), the front of incubator (1) articulates there is door (16) that opens and shuts.
7. A tumor cell culture apparatus according to claim 1, wherein: the bottom fixedly connected with of the flexible end of flexible pipe (14) fills up sealed (25), the inside wall of incubator (1) is fixed to be inlayed and is had bearing (15), the inner circle fixed connection of the one end that first motor (3) were kept away from in lead screw (4) and bearing (15).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021125634.7U CN212800392U (en) | 2020-06-17 | 2020-06-17 | Tumor cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021125634.7U CN212800392U (en) | 2020-06-17 | 2020-06-17 | Tumor cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212800392U true CN212800392U (en) | 2021-03-26 |
Family
ID=75097071
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021125634.7U Expired - Fee Related CN212800392U (en) | 2020-06-17 | 2020-06-17 | Tumor cell culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212800392U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113930341A (en) * | 2021-11-16 | 2022-01-14 | 郑州大学第一附属医院 | Integrated esophageal cancer tumor cell culture and separation system |
-
2020
- 2020-06-17 CN CN202021125634.7U patent/CN212800392U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113930341A (en) * | 2021-11-16 | 2022-01-14 | 郑州大学第一附属医院 | Integrated esophageal cancer tumor cell culture and separation system |
| CN113930341B (en) * | 2021-11-16 | 2023-05-12 | 郑州大学第一附属医院 | Esophageal cancer tumor cell culture separation system |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210326 |