CN113214990A - Hematopoietic stem cell amplification culture extraction integrated equipment - Google Patents

Hematopoietic stem cell amplification culture extraction integrated equipment Download PDF

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Publication number
CN113214990A
CN113214990A CN202110524656.3A CN202110524656A CN113214990A CN 113214990 A CN113214990 A CN 113214990A CN 202110524656 A CN202110524656 A CN 202110524656A CN 113214990 A CN113214990 A CN 113214990A
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fixedly connected
connecting pipe
pipe
hematopoietic stem
upper cover
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陈川
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Beijing Totem Hunting Technology Co ltd
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Beijing Totem Hunting Technology Co ltd
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto 
    • B08B9/08—Cleaning containers, e.g. tanks
    • B08B9/087—Cleaning containers, e.g. tanks by methods involving the use of tools, e.g. brushes, scrapers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto 
    • B08B9/08—Cleaning containers, e.g. tanks
    • B08B9/093—Cleaning containers, e.g. tanks by the force of jets or sprays
    • B08B9/0936—Cleaning containers, e.g. tanks by the force of jets or sprays using rotating jets
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    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls

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Abstract

The invention discloses integrated equipment for hematopoietic stem cell amplification culture and extraction, and relates to the technical field of cell culture equipment. The utility model provides an integration equipment is drawed in hematopoietic stem cell amplification culture, which comprises an outer cylinder, the lower surface circumference edge fixedly connected with supporting leg of urceolus, the inside of urceolus is provided with the inner tube, the downside inner wall upper surface of the lower fixed surface connection urceolus of inner tube, the upper surface fixedly connected with upper cover ring of urceolus, the upper surface of the inboard edge fixedly connected with inner tube of lower surface of upper cover ring, the upper surface outside edge fixedly connected with side cap of upper cover ring department, the left surface lower extreme of inner tube runs through and has seted up the opening and at opening department fixedly connected with discharge pipe one, one side opening part sliding connection that the outer tube was kept away from to discharge pipe one has sealed lid one, the hematopoietic stem cell amplification culture who has solved the integration draws equipment after having drawn the cell sap, the remaining cell sap of equipment inner wall can not wash thoroughly very conveniently, produce the problem of bacterium easily.

Description

Hematopoietic stem cell amplification culture extraction integrated equipment
Technical Field
The invention relates to the technical field of cell culture equipment, in particular to integrated equipment for hematopoietic stem cell amplification culture and extraction.
Background
The hematopoietic stem cells are adult stem cells in a blood system, are heterogeneous groups, have long-term self-renewal capacity and differentiation potential into various mature blood cells, are adult stem cells with the longest research history and the deepest research history, have important guiding significance for researching various stem cells including tumor stem cells, and need to be subjected to amplification culture on the hematopoietic stem cells firstly during experimental research and then extract the cells.
However, after the integrated hematopoietic stem cell expansion culture extraction equipment extracts the cell sap, the cell sap remained on the inner wall of the equipment cannot be cleaned thoroughly, bacteria are easily generated, and the cell sap is polluted when the equipment is used next time, so that the culture fails.
Disclosure of Invention
The invention aims to provide an integrated device for expansion culture and extraction of hematopoietic stem cells, which solves the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: an integrated device for hematopoietic stem cell amplification culture and extraction comprises an outer barrel, wherein supporting legs are fixedly connected to the circumferential edge of the lower surface of the outer barrel, an inner barrel is arranged in the outer barrel, the lower end of the left side surface of the inner barrel is provided with a through hole in a penetrating manner, a first discharge pipe is fixedly connected to the through hole, a first sealing cover is connected to the opening of one side of the first discharge pipe, which is far away from the outer barrel in a sliding manner, the lower end of the left side surface of the outer barrel is fixedly connected to the circumferential side surface of the first discharge pipe, a second discharge pipe is arranged on the right side surface of the inner barrel, which is far away from the through hole in a fixedly connected manner, a second sealing cover is connected to the opening of one side of the second discharge pipe, which is far away from the outer barrel in a sliding manner, the circumferential side surface of the second discharge pipe, which is through the right side surface of the outer barrel, a first connecting pipe is arranged in the inner barrel, and the lower surface of the first connecting pipe is fixedly connected with a second connecting pipe, the equal fixedly connected with slide bar in side about connecting pipe two, the equal sliding connection in surface lower extreme of connecting pipe two and slide bar has connecting pipe three, the equal fixedly connected with stirring of side lower extreme hollow rod around connecting pipe three, the left surface of hollow rod runs through and has seted up the opening and at opening fixedly connected with spray tube of department, hollow rod and spray tube use connecting pipe three to be central symmetry setting as the axle center in the stirring, the lower surface of connecting pipe three rotates the downside inner wall upper surface center department of connecting the urceolus, the downside inner wall upper surface of the lower surface sliding connection urceolus of hollow rod in the stirring, the top of connecting pipe one is provided with water pump box and upper cover plate, be provided with the electrothermal tube between urceolus and the inner tube.
Preferably, the lower surface of the inner cylinder is fixedly connected with the upper surface of the inner wall of the lower side of the outer cylinder, the upper surface of the outer cylinder is fixedly connected with an upper cover ring, the inner side edge of the lower surface of the upper cover ring is fixedly connected with the upper surface of the inner cylinder, the outer side edge of the upper surface of the upper cover ring is fixedly connected with a side cover, and the first discharge pipe and the second discharge pipe are both in a bent neck shape.
Preferably, the upper ends of the front side and the rear side of the second connecting pipe are fixedly connected with a cross rod, one side of the lower surface of the cross rod, which is far away from the second connecting pipe, is fixedly connected with a first spring telescopic rod, the lower surface of the first spring telescopic rod is fixedly connected with a scraper, the lower surface of the scraper is connected with the upper surface of a hollow rod in stirring in a sliding manner, the inner side of the scraper is connected with the outer side surface of a third connecting pipe in a sliding manner, the outer side of the scraper is connected with the inner wall of the inner cylinder in a sliding manner, a liquid adding valve is fixedly connected with the center of the upper surface of a water pump box, the front side and the rear side of the lower surface of the water pump box are fixedly connected with a second spring telescopic rod, the center of the lower surface of the water pump box is fixedly connected with a fourth connecting pipe, the lower end of the outer side surface of the fourth connecting pipe is fixedly connected with a first baffle ring, the outer side surface of the first baffle ring is slidably connected with a threaded pipe, and the upper surface of the threaded pipe is fixedly connected with a second baffle ring, the upper surface of the first baffle ring is rotatably connected with the lower surface of the inner side of the second baffle ring, and the lower surface of the threaded pipe is fixedly connected with the upper surface of the first connecting pipe.
Preferably, handles are fixedly connected to the left end and the right end of the upper surface of the upper cover plate, a sealing ring plate is fixedly connected to the lower surface of the upper cover plate, a clamping rod is fixedly connected to the lower surface of the sealing ring plate, a groove is formed in the upper surface of the upper cover ring, the lower end of the side surface of the clamping rod is slidably connected with the inner wall of the groove in the upper surface of the upper cover ring, the circumferential side surface of the threaded pipe penetrates through the center of the upper surface of the upper cover plate in a threaded connection mode, the upper surface of the upper cover plate is fixedly connected with the lower surface of the spring telescopic rod II, the circumferential edge of the lower surface of the upper cover plate is attached to the upper surface of the side cover, the lower surface of the electric heating pipe is fixedly connected with the upper surface of the outer cylinder, the electric heating pipe is arranged in an annular array mode in a gap between the outer cylinder and the inner cylinder, the cross section of the stirring hollow rod is arc-shaped, the bending direction of the upper end of the cross section of the stirring hollow rod is the same as the lower end of the arc-shaped, the cross section upper end of stirring cavity pole is the plane, the oblique below in the outside that the connecting pipe three was kept away from to the opening orientation of hydrojet pipe.
Compared with the prior art, the invention has the beneficial effects that:
(1) the integrated equipment for hematopoietic stem cell amplification culture and extraction is characterized in that a liquid spraying pipe and a scraper are arranged, a water pump box is opened, cleaning liquid is injected into a four-way threaded pipe through a connecting pipe, the cleaning liquid sequentially passes through the threaded pipe, a first connecting pipe, a second connecting pipe, a third connecting pipe and a stirring hollow rod, then the cleaning liquid is sprayed out from an opening of the liquid spraying pipe, the opening of the liquid spraying pipe faces to the outer side inclined lower part far away from the third connecting pipe, the stirring hollow rod and the liquid spraying pipe are arranged in central symmetry by taking the third connecting pipe as an axis, the third connecting pipe can be pushed to rotate when the cleaning liquid is sprayed out at a high speed, so that the threaded pipe is driven to rotate, the cleaning liquid sprayed out of the liquid spraying pipe can wash the inner wall of an inner barrel and stay in the inner barrel to enable the liquid level to rise gradually, the cleaning liquid flows into the second discharging pipe until the cleaning liquid level is higher than the left opening of the second discharging pipe, the inner wall of the inner barrel is preliminarily cleaned, so that residual culture liquid is washed away, the subsequent cleaning is convenient.
(2) This hematopoietic stem cell amplification culture draws integration equipment through setting up the scraper blade, washes the inner tube inner wall at spray tube blowout washing liquid after, along with the decline of connecting pipe three, the scraper blade is also following the rotation and is descending, and the scraper blade outside surface scrapes the clearance to the inner tube inner wall that has washed, will remain the liquid and thoroughly scrape from the inner tube inner wall, further improves the cleaning performance.
(3) This hematopoietic stem cell amplification culture draws integration equipment, through setting up the stirring hollow rod, the cross sectional shape of stirring hollow rod is provided with the arch for arc and cambered surface middle part, cambered surface upper end direction of buckling is the plane with the same and upper end of cambered surface lower extreme orientation, can mix the washing liquid and utilize centrifugal force to make the washing liquid flow to inner tube inner wall when the stirring hollow rod rotates, wash away the inner tube inner wall, the protruding improvement of stirring hollow rod cambered surface stirs the effect of indiscriminate rivers, the part of buckling of stirring hollow rod cambered surface upper end can live the rivers pocket, further improve the effect that promotes rivers to inner tube inner wall direction, improve the cleaning performance.
(4) This hematopoietic stem cell amplification culture draws integration equipment, through setting up the hollow rod of stirring, after liquid level is higher than discharge pipe two and the washing liquid flows into discharge pipe two in the inner tube, the hollow rod level of stirring is also less than liquid level, and the hollow rod of stirring further pushes the washing liquid into discharge pipe two in, thereby improves the velocity of flow and improves the washing effect to discharge pipe two inner walls.
(5) This hematopoietic stem cell amplification culture draws integration equipment, through setting up the stirring hollow rod, after the three bottoms of connecting pipe contacted urceolus bottoms, the stirring hollow rod lower surface slides along urceolus bottom upper surface and scrapes off the residual liquid of urceolus downside inner wall upper surface, and the rotation of cooperation stirring hollow rod self makes residual liquid follow the washing liquid and discharges from discharge pipe one, can fully clean the residual liquid of brushing the bottom, improves the cleaning performance.
(6) This hematopoietic stem cell amplification culture draws integration equipment, through setting up the scraper blade, along with the rotation of connecting pipe three lasts going on, the spray tube lasts to pouring into the washing liquid in the inner tube, and the scraper blade pushes down the liquid level, and the washing liquid can't flow to the scraper blade top to make the interior washing liquid hydraulic pressure of inner tube grow gradually, until dashing the sealed lid on the discharge pipe one, improve the velocity of flow in the twinkling of an eye of washing liquid, thereby improve the washing effect of washing liquid to a discharge pipe inner wall.
(7) This hematopoietic stem cell amplification culture draws integration equipment, through setting up the scraper blade, when connecting pipe three keeps the pivoted, connecting pipe two and slide bar begin to glide and slide in the three inner walls of connecting pipe, horizontal pole and spring telescopic link promote the scraper blade to move down, scrape the remaining liquid on the three surfaces of connecting pipe again when the scraper blade moves down, avoid device self to remain, improve the cleaning performance.
(8) This hematopoietic stem cell amplification culture draws integration equipment, through setting up the stirring cavity pole, the cambered surface upper end of stirring cavity pole direction of buckling is the plane with the same and upper end of cambered surface lower extreme orientation, and the attached residual liquid of scraper blade lower surface is scraped by stirring cavity pole upper end, then is discharged in discharge pipe one, is discharged at last, reduces the attached residue of residual liquid, improves the cleaning performance.
(9) This hematopoietic stem cell amplification culture draws integration equipment, through setting up spring telescopic link one, spring telescopic link one makes the scraper blade lower surface can also rotate one end time after the contact stirring hollow rod upper surface, and the time of brushing is cleaned in the extension, improves the effect of stirring hollow rod clearance scraper blade lower surface.
(10) This hematopoietic stem cell amplification culture draws integration equipment sets up to bent neck form through the shape with discharge pipe one and discharge pipe two, is the bent neck bottle experiment of pasteur on the same line, and the air can follow discharge pipe two and get into in the inner tube and the bacterium with float and sink unable along the two openings of discharge pipe two and rise to the department of buckling of discharge pipe two to in unable entering into the inner tube, just also avoided inner tube internal environment when pouring into cell sap and culture solution to receive bacterial contamination, improved the culture effect.
(11) This hematopoietic stem cell amplification culture draws integration equipment, through setting up sealed lid one, when improving the device leakproofness, when pouring into washing liquid belt cleaning device inner wall, sealed lid one can also block the opening of discharge pipe one and avoid the washing liquid directly to flow out, make the washing liquid level in the inner tube can rise to discharge pipe two places, conveniently wash the inner wall of discharge pipe two, along with the scraper blade lasts to move down, water pressure in the inner tube is more and more big until sealed lid is automatic to be washed away, when utilizing the water pressure that increases to improve the washing effect to discharge pipe inner wall, do not need artifical supplementary operation, the work efficiency is improved, the human labor is lightened.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the internal structure of the present invention;
FIG. 3 is a schematic view of the structural connection of the stirring hollow rod of the present invention;
FIG. 4 is an enlarged view of a portion of FIG. 1;
FIG. 5 is an enlarged view of a portion of FIG. 2 at B according to the present invention.
In the figure: 101 outer cylinder, 102 supporting leg, 103 inner cylinder, 104 upper cover ring, 105 side cover, 106 discharge pipe I, 107 sealing cover I, 108 discharge pipe II, 109 sealing cover II, 201 connecting pipe I, 202 connecting pipe II, 203 sliding rod, 204 connecting pipe III, 205 stirring hollow rod, 206 liquid spraying pipe, 207 cross rod, 208 spring telescopic rod I, 209 scraping plate, 301 water pump box, 302 liquid adding valve, 303 spring telescopic rod II, 304 connecting pipe IV, 305 retaining ring I, 306 threaded pipe, 307 retaining ring II, 401 upper cover plate, 402 handle, 403 sealing ring plate, 404 clamping rod and 5 electric heating pipe.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, the present invention provides a technical solution: an integrated device for hematopoietic stem cell amplification culture and extraction comprises an outer cylinder 101, wherein a supporting leg 102 is fixedly connected to the circumferential edge of the lower surface of the outer cylinder 101, an inner cylinder 103 is arranged inside the outer cylinder 101, the lower end of the left side surface of the inner cylinder 103 is provided with a through hole in a penetrating manner and is fixedly connected with a first discharge pipe 106 at the through hole, a first sealing cover 107 is slidably connected to an opening of one side of the first discharge pipe 106, which is far away from the outer cylinder 101, the circumferential side surface of the first discharge pipe 106 is provided with a through hole in a penetrating manner and is fixedly connected with the lower end of the left side surface of the outer cylinder 101, the right side surface of the inner cylinder 103 is provided with a through hole in a penetrating manner and is fixedly connected with a second discharge pipe 108 at the through hole, a second sealing cover 109 is slidably connected to an opening of one side of the second discharge pipe 108, the circumferential side surface of the second discharge pipe 108 is fixedly connected with the right side surface of the outer cylinder 101, a first connecting pipe 201 is arranged inside of the inner cylinder 103, and a second connecting pipe 202 is fixedly connected to the lower surface of the first connecting pipe 201, the left side and the right side of the second connecting pipe 202 are fixedly connected with a sliding rod 203, the lower ends of the surfaces of the second connecting pipe 202 and the sliding rod 203 are slidably connected with a third connecting pipe 204, the lower ends of the front side and the rear side of the third connecting pipe 204 are fixedly connected with a stirring hollow rod 205, the left side of the stirring hollow rod 205 is provided with a through hole in a penetrating way and is fixedly connected with a liquid spraying pipe 206 at the through hole, the stirring hollow rod 205 and the liquid spraying pipe 206 are arranged in central symmetry by taking the third connecting pipe 204 as an axis, the lower surface of the third connecting pipe 204 is rotatably connected with the center of the upper surface of the lower inner wall of the outer cylinder 101, the lower surface of the stirring hollow rod 205 is slidably connected with the upper surface of the lower inner wall of the outer cylinder 101, a water pump box 301 and an upper cover plate 401 are arranged above the first connecting pipe 201, an electric heating pipe 5 is arranged between the outer cylinder 101 and the inner cylinder 103, the lower surface of the inner cylinder 103 is fixedly connected with the upper surface of the lower inner wall of the outer cylinder 101, and an upper cover ring 104 is fixedly connected with the upper surface of the outer cylinder 101, the inner side edge of the lower surface of the upper cover ring 104 is fixedly connected with the upper surface of the inner cylinder 103, the outer side edge of the upper surface of the upper cover ring 104 is fixedly connected with the side cover 105, and the first discharge pipe 106 and the second discharge pipe 108 are both in a curved neck shape.
The upper ends of the front side and the rear side of the second connecting pipe 202 are fixedly connected with a cross rod 207, one side of the lower surface of the cross rod 207, which is far away from the second connecting pipe 202, is fixedly connected with a first spring telescopic rod 208, the lower surface of the first spring telescopic rod 208 is fixedly connected with a scraper 209, the lower surface of the scraper 209 is slidably connected with the upper surface of a stirring hollow rod 205, the inner side circumferential surface of the scraper 209 is slidably connected with the outer circumferential side surface of a third connecting pipe 204, the outer side circumferential surface of the scraper 209 is slidably connected with the inner wall of the inner cylinder 103, the center of the upper surface of the water pump box 301 is fixedly connected with a liquid adding valve 302, the front side and the rear side of the lower surface of the water pump box 301 are fixedly connected with a second spring telescopic rod 303, the center of the lower surface of the water pump box 301 is fixedly connected with a fourth connecting pipe 304, the lower end of the circumferential side surface of the fourth connecting pipe 304 is fixedly connected with a first retaining ring 305, the outer circumferential side surface of the first retaining ring 305 is slidably connected with a threaded pipe 306, the upper surface of the threaded pipe 306 is fixedly connected with a second retaining ring 307, the upper surface of the first baffle ring 305 is rotatably connected with the lower surface of the inner side of the second baffle ring 307, the lower surface of the threaded pipe 306 is fixedly connected with the upper surface of the first connecting pipe 201, the left end and the right end of the upper surface of the upper cover plate 401 are fixedly connected with handles 402, the lower surface of the upper cover plate 401 is fixedly connected with a sealing ring plate 403, the lower surface of the sealing ring plate 403 is fixedly connected with a clamping rod 404, the upper surface of the upper cover ring 104 is provided with a groove, the lower end of the side surface of the clamping rod 404 is slidably connected with the inner wall of the groove on the upper surface of the upper cover ring 104, the circumferential side surface of the threaded pipe 306 penetrates through the center of the upper surface of the upper cover plate 401, the upper surface of the upper cover plate 401 is fixedly connected with the lower surface of the second spring telescopic rod 303, the circumferential edge of the lower surface of the upper cover plate 401 is attached with, the cross section of the stirring hollow rod 205 is arc-shaped, the middle part of the arc surface is provided with a bulge, the bending direction of the upper end of the arc surface of the cross section of the stirring hollow rod 205 is the same as the direction of the lower end of the arc surface, the upper end of the cross section of the stirring hollow rod 205 is a plane, and the opening of the liquid spraying pipe 206 faces the outer side oblique lower side far away from the connecting pipe III 204, so that the problems that the residual cell sap on the inner wall of the integrated hematopoietic stem cell amplification culture extraction equipment cannot be conveniently and thoroughly cleaned and bacteria are easily generated after the cell sap is extracted by the integrated hematopoietic stem cell amplification culture extraction equipment are solved.
When in use, the second sealing cover 109 is taken down from the opening of the second discharge pipe 108 in a sliding way, the level of the second discharge pipe 108 is higher than that of the first discharge pipe 106, hematopoietic stem cells and culture solution are injected from the second discharge pipe 108 by the syringe, the needle tube of the syringe is inserted into the second discharge pipe 108, the mixed solution is sprayed out of the needle tube, flows through the bent part of the second discharge pipe 108 and then flows into the inner cylinder 103, then the second sealing cover 109 is sleeved on the opening of the second sealing cover 109 again, because the second discharge pipe 108 is in a bent neck shape, similarly to the bent neck bottle experiment of Pasteur, air can enter the inner cylinder 103 from the second discharge pipe 108, bacteria and floating and sinking can not rise to the bent part of the second discharge pipe 108 along the opening of the second discharge pipe 108, and can not enter the inner cylinder 103, thereby avoiding bacterial pollution to the inner environment of the inner cylinder 103 when injecting cell solution and culture solution, improving the culture effect, and the level of the injected mixed solution is lower than the level of the opening of the left side of the second discharge pipe 108, which is connected with the inner wall of the inner cylinder 103 The upper cover plate 401 covers the upper cover ring 104, the clamping rod 404 is inserted into a groove on the upper cover ring 104, the edge of the lower surface of the upper cover plate 401 is attached to the upper surface of the side cover 105 to isolate the outside air, the sealing ring plate 403 plays a role in secondary isolation to improve the sealing effect, the electrothermal tube 5 is opened to heat and preserve the temperature inside the inner cylinder 103, so that the hematopoietic stem cells inside can be successfully amplified and cultured, the electrothermal tube 5 is closed after the culture is finished, the sealing cover I107 is slidingly taken down to open the left opening of the discharge pipe I106, then the whole device is inclined to pour out the mixed liquid from the discharge pipe I106, the arrangement of the discharge pipe I106 is the same as that of the discharge pipe II 108, bacteria and impurities cannot enter the inner wall of the discharge pipe I106, so that the inner wall of the discharge pipe I106 is prevented from being polluted, when the device is idle, the sealing cover I107 is sleeved on the, at this time, the mixed liquid remains on the inner walls of the inner cylinder 103, the second discharge pipe 108, and the first discharge pipe 106.
The second sealing cover 109 is slid and taken down from the second discharge pipe 108, the first sealing cover 107 is sleeved on the first discharge pipe 106, then the water pump tank 301 is opened, cleaning liquid is injected into the threaded pipe 306 through the fourth connecting pipe 304, the cleaning liquid sequentially passes through the threaded pipe 306, the first connecting pipe 201, the second connecting pipe 202, the third connecting pipe 204 and the stirring hollow rod 205 and then is sprayed out from the opening of the liquid spraying pipe 206, the opening of the liquid spraying pipe 206 faces to the lower side of the outer side far away from the third connecting pipe 204, the stirring hollow rod 205 and the liquid spraying pipe 206 are arranged in central symmetry by taking the third connecting pipe 204 as an axis, when the cleaning liquid is sprayed out at a high speed, the third connecting pipe 204 can be pushed to rotate, so that the threaded pipe 306 is driven to rotate, the upper cover plate 401 is clamped by the clamping rod 404 and the groove of the upper cover ring 104 and cannot rotate, so that the threaded pipe 306 spirally rotates and descends, the upper surface of the first blocking ring 305 is blocked by the second blocking ring 307, so that the water pump tank 301 is synchronously moved downwards, the spring in the second spring telescopic rod 303 is compressed and shortened, the cleaning liquid sprayed out of the liquid spraying pipe 206 can wash the inner wall of the inner cylinder 103 and stays in the inner cylinder 103 to enable the liquid level to gradually rise until the cleaning liquid is higher than the left opening of the second discharge pipe 108 and flows into the second discharge pipe 108, after the cleaning liquid sprayed out of the liquid spraying pipe 206 washes the inner wall of the inner cylinder 103, the scraper 209 also rotates and descends along with the descending of the third connecting pipe 204, the outer side surface of the scraper 209 scrapes and cleans the washed inner wall of the inner cylinder 103, the residual liquid is thoroughly scraped from the inner wall of the inner cylinder 103, the cleaning effect is further improved, the cross section of the stirring hollow rod 205 is arc-shaped, the middle part of the arc-shaped surface is provided with a bulge, the bending direction of the upper end of the arc-shaped surface is the same as the lower end of the arc-shaped surface, the upper end of the arc-shaped surface is a plane, when the stirring hollow rod 205 rotates, the cleaning liquid can be stirred to enable the cleaning liquid to flow to the inner wall of the inner cylinder 103 by utilizing centrifugal force, and wash the inner wall of the inner cylinder 103, the effect of stirring rivers is improved to the arch of stirring hollow rod 205 cambered surface, the bending part of stirring hollow rod 205 cambered surface upper end can be lived rivers in the pocket, further improve the effect that promotes rivers to the inner wall direction of inner tube 103, after liquid level height is higher than second 108 of discharge pipe and the washing liquid flows into second 108 of discharge pipe in the inner tube 103, the hollow rod 205 level also is less than liquid level height in the stirring, the hollow rod 205 in the stirring further pushes the washing liquid into second 108 of discharge pipe, thereby improve the velocity of flow and improve the washing effect to second 108 inner walls of discharge pipe, then these washing liquids flow out from second 108 right side opening of discharge pipe.
As the connecting pipe III 204 continues to rotate, the liquid spraying pipe 206 continues to inject the cleaning liquid into the inner cylinder 103, the scraper 209 presses the liquid level downwards, the cleaning liquid cannot flow above the scraper 209, so that the hydraulic pressure of the cleaning liquid in the inner cylinder 103 gradually increases until the sealing cover I107 on the discharge pipe I106 is flushed, then the cleaning liquid flows out from the left opening of the discharge pipe I106, when the bottom of the connecting pipe III 204 contacts the bottom of the outer cylinder 101, the lower surface of the stirring hollow rod 205 slides along the upper surface of the bottom of the outer cylinder 101 to scrape off the residual liquid on the upper surface of the inner wall of the lower side of the outer cylinder 101, the residual liquid is discharged from the discharge pipe I106 along with the cleaning liquid by matching with the rotation of the stirring hollow rod 205, at the moment, the connecting pipe III 204 keeps rotating, the connecting pipe II 202 and the sliding rod 203 start to slide downwards into the inner wall, when the scraper 209 moves downwards, the residual liquid on the surface of the connecting pipe III 204 is scraped, when the lower surface of the scraper 209 contacts the upper surface of the stirring hollow rod 205, the spring in the spring telescopic rod I208 is compressed and shortened to generate downward pressure on the scraper 209, the bending direction of the upper end of the cambered surface of the stirring hollow rod 205 is the same as the direction of the lower end of the cambered surface, the upper end of the cambered surface is in a plane, the residual liquid attached to the lower surface of the scraper 209 is scraped by the upper end of the stirring hollow rod 205, then the residual liquid is discharged into the discharge pipe I106, and finally the residual liquid is discharged, the spring telescopic rod I208 enables the lower surface of the scraper 209 to rotate for a period of time after contacting the upper surface of the stirring hollow rod 205, the cleaning time is prolonged, the effect of the stirring hollow rod 205 on cleaning the lower surface of the scraper 209 is improved, when the spring in the spring telescopic rod I208 is compressed to the shortest, the connecting pipe III 204 cannot rotate any more, the water pump tank 301 is closed, and then the inclined device pours out the residual cleaning liquid in the inner cylinder 103, at the moment, the inner walls of the inner cylinder 103, the second discharge pipe 108 and the first discharge pipe 106 are completely cleaned, so that the problems that after the cell sap is extracted by the integrated hematopoietic stem cell amplification culture extraction equipment, the cell sap residual on the inner wall of the equipment cannot be conveniently and completely cleaned, bacteria are easily generated, the cell sap is polluted when the equipment is used next time, the culture fails are solved, the operation is convenient and rapid, and the cleaning effect is good are solved.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (9)

1. The utility model provides an integrated equipment is drawed in hematopoietic stem cell amplification culture, includes urceolus (101), the lower surface circumference edge fixedly connected with supporting leg (102) of urceolus (101), the inside of urceolus (101) is provided with inner tube (103), the left surface lower extreme of inner tube (103) runs through and has seted up the opening and at opening fixedly connected with discharge pipe (106), its characterized in that: the outer cylinder is characterized in that a first sealing cover (107) is connected to an opening on one side, away from the outer cylinder (101), of the first discharge pipe (106) in a sliding mode, the circumferential side face of the first discharge pipe (106) penetrates through the lower end of the left side face of the outer cylinder (101) in a fixed mode, a through opening is formed in the right side face of the inner cylinder (103) in a penetrating mode, a second discharge pipe (108) is fixedly connected to the opening on one side, away from the outer cylinder (101), of the second discharge pipe (108) in a sliding mode, a second sealing cover (109) is connected to the opening on one side, across the right side face of the outer cylinder (101) in a fixed mode, a first connecting pipe (201) is arranged inside the inner cylinder (103), a second connecting pipe (202) is fixedly connected to the lower surface of the first connecting pipe (201), sliding rods (203) are fixedly connected to the left side face and the right side face of the second connecting pipe (202), and sliding rods (203) are connected to the lower ends of the surfaces of the second connecting pipe (202) and the sliding rods (203) in a third connecting pipe (204) in a sliding mode, the equal fixedly connected with stirring of side lower extreme in the front and back of connecting pipe three (204) pole (205), the left surface of stirring pole (205) runs through and has seted up the opening and at opening department fixedly connected with spray pipe (206), stirring pole (205) and spray pipe (206) are central symmetry setting for the axle center with connecting pipe three (204), the lower surface of connecting pipe three (204) rotates the downside inner wall upper surface center department of connecting urceolus (101), the downside inner wall upper surface of lower surface sliding connection urceolus (101) of stirring pole (205), the top of connecting pipe one (201) is provided with water pump box (301) and upper cover plate (401), be provided with electrothermal tube (5) between urceolus (101) and inner tube (103).
2. The integrated hematopoietic stem cell expansion, culture and extraction device of claim 1, wherein: the lower surface fixed connection urceolus (101) of inner tube (103) downside inner wall upper surface, the last fixed surface of urceolus (101) is connected with upper cover ring (104), the upper surface of the inboard edge fixed connection inner tube (103) of lower surface of upper cover ring (104), the upper surface outside edge department fixed connection side cap (105) of upper cover ring (104).
3. The integrated hematopoietic stem cell expansion, culture and extraction device of claim 1, wherein: the first discharge pipe (106) and the second discharge pipe (108) are both in a curved neck shape.
4. The integrated hematopoietic stem cell expansion, culture and extraction device of claim 1, wherein: the equal fixedly connected with horizontal pole (207) in side upper end around connecting pipe two (202), one side fixedly connected with spring telescopic link (208) of connecting pipe two (202) is kept away from to the lower surface of horizontal pole (207), the lower fixed connection of spring telescopic link (208) has scraper blade (209), the upper surface of hollow pole (205) in the lower surface sliding connection stirring of scraper blade (209), the circumference lateral surface of the inboard circumference surface sliding connection connecting pipe three (204) of scraper blade (209), the inner wall of the outside circumference surface sliding connection inner tube (103) of scraper blade (209).
5. The integrated hematopoietic stem cell expansion, culture and extraction device of claim 1, wherein: fixedly connected with liquid feeding valve (302) is located at the upper surface center of water pump case (301), the equal fixedly connected with spring telescopic link two (303) of rear side before the lower surface of water pump case (301), the lower surface center fixedly connected with connecting pipe four (304) of water pump case (301), the circumference side lower extreme of connecting pipe four (304) is fixed to be cup jointed and is kept off ring one (305), the circumference lateral surface sliding connection that keeps off ring one (305) has screwed pipe (306), the upper surface fixed connection of screwed pipe (306) keeps off ring two (307), the upper surface rotation of keeping off ring one (305) connects the inboard lower surface that keeps off ring two (307), the upper surface of the lower fixed connection connecting pipe one (201) of screwed pipe (306).
6. The integrated apparatus for hematopoietic stem cell expansion culture and extraction according to claim 5, wherein: the utility model discloses a spring telescopic link, including upper cover plate (401), upper cover plate's the upper surface of upper cover plate (401), the equal fixedly connected with handle in both ends (402) about the upper surface of upper cover plate (401), the lower fixed surface of upper cover plate (401) is connected with sealed crown plate (403), the lower fixed surface of sealed crown plate (403) is connected with kelly (404), the upper surface of upper cover plate (104) is seted up flutedly, the recess inner wall of upper cover ring (104) upper surface is connected in the side lower extreme sliding connection of kelly (404), the circumference side of screwed pipe (306) runs through the upper surface center department of threaded connection upper cover plate (401), the lower surface of upper cover plate (401) upper surface fixed connection spring telescopic link two (303), the lower surface circumference.
7. The integrated hematopoietic stem cell expansion, culture and extraction device of claim 1, wherein: the lower surface of the electric heating tube (5) is fixedly connected with the upper surface of the outer cylinder (101), and the electric heating tubes (5) are arranged in a gap between the outer cylinder (101) and the inner cylinder (103) in an annular array.
8. The integrated hematopoietic stem cell expansion, culture and extraction device of claim 1, wherein: the cross sectional shape of stirring cavity pole (205) is provided with the arch for arc and cambered surface middle part, the cross section cambered surface upper end of stirring cavity pole (205) direction of buckling is the same with cambered surface lower extreme orientation, the cross section upper end of stirring cavity pole (205) is the plane.
9. The integrated hematopoietic stem cell expansion, culture and extraction device of claim 1, wherein: the opening of the liquid spray pipe (206) faces to the outer side of the third connecting pipe (204) and is obliquely downward.
CN202110524656.3A 2021-05-13 2021-05-13 Hematopoietic stem cell amplification culture extraction integrated equipment Withdrawn CN113214990A (en)

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Application Number Priority Date Filing Date Title
CN202110524656.3A CN113214990A (en) 2021-05-13 2021-05-13 Hematopoietic stem cell amplification culture extraction integrated equipment

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113856432A (en) * 2021-10-28 2021-12-31 北方联合电力有限责任公司呼和浩特金桥热电厂 Power plant desulfurizing tower
CN113897278A (en) * 2021-09-26 2022-01-07 李同辉 Nutrient solution adding equipment used in biological cultivation
CN113930341A (en) * 2021-11-16 2022-01-14 郑州大学第一附属医院 Integrated esophageal cancer tumor cell culture and separation system

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113897278A (en) * 2021-09-26 2022-01-07 李同辉 Nutrient solution adding equipment used in biological cultivation
CN113856432A (en) * 2021-10-28 2021-12-31 北方联合电力有限责任公司呼和浩特金桥热电厂 Power plant desulfurizing tower
CN113856432B (en) * 2021-10-28 2023-11-17 北方联合电力有限责任公司呼和浩特金桥热电厂 Desulfurizing tower of power plant
CN113930341A (en) * 2021-11-16 2022-01-14 郑州大学第一附属医院 Integrated esophageal cancer tumor cell culture and separation system

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