CN209237455U - A kind of cell filtration system of cell filtration component and its composition - Google Patents

Abstract

The utility model discloses the cell filtration systems of a kind of cell filtration component and its composition, the cell filtration component includes filter, filter housing and centrifuge tube, the filter is vertically arranged for one and opening up groove body, the slot bottom of the filter are covered with sieve pore；The filter housing is vertically arranged and inner hollow shell, the upper end of the filter housing is equipped with and its internal the first tubular interface for being connected to and matching with the filter, its lower end is equipped with and its internal the second tubular interface for being connected to and matching with the centrifuge tube nozzle, the tubular interface of third being connected to there are two being set on its side wall with its inside, the lower end of the filter is extend into from top to bottom in the described first tubular interface, and it is tightly connected with it, the centrifuge tube is vertically arranged in the lower section of the filter housing, the second tubular interface is extend into from top to bottom in the nozzle of the centrifuge tube, and it is tightly connected with it.Its structure is simple, can carry out negative pressure filtration.

Description

A kind of cell filtration system of cell filtration component and its composition

Technical field

The utility model belongs to the cell of medical equipment field more particularly to a kind of cell filtration component and its composition Filtration system.

Background technique

When carrying out that flow cytometer carries out cell, nucleus or other organelles are analyzed, first need to sample into Row filtering, removes large granular impurity therein, so that the filtered fluid containing target cell or nucleus or organelle is obtained by filtration, And gained filtered fluid usually also needs to be centrifuged to collect target cell or nucleus or organelle.

Sample filter chamber is usually first to filter sample into a container at present, then again turns the filtered fluid in container It moves in centrifuge tube, process is complicated, and is easy to appear germ contamination, although it is straight to have a kind of filter of patent report at present It connects in filtrate to centrifuge tube, but speed of its filtering is slower.

Utility model content

In order to solve the above-mentioned technical problem, it is simple to be the provision of a kind of structure for one of the purpose of this utility model, and at This cheap, repeatedly used cell filtration component.

To achieve the goals above, the technical solution of the utility model is as follows: a kind of cell filtration component, including filtering Device, filter housing and centrifuge tube；The filter housing is inner hollow shell, the upper end of the filter housing be equipped with its it is internal be connected to and The the first tubular interface matched with the filter, lower end, which is equipped with, to be connected to its inside and matches with the centrifuge tube nozzle The the second tubular interface closed, set on side wall there are two with its internal tubular interface of third be connected to, the filter seal pacifies Mounted in the described first tubular interface, the centrifuge tube is vertically arranged in the lower section of the filter housing, the second tubular interface It extend into the nozzle of the centrifuge tube, and is tightly connected with it.

The beneficial effect of above-mentioned technical proposal is: its structure is simple, and filter is mounted in the first tubular interface, will Sample is added in filter and filters, and filtered fluid is added dropwise in filter housing and is flowed into centrifuge tube through the second tubular interface, In, one in two tubular interfaces of third can block, the external negative pressure equipment of the tubular interface of another third, in this way, in mistake It is at negative pressure state at filter housing and centrifuge tube, is conducive to the rate of filtration of the filtered fluid in accelofilter.

Further, the upper end of filter described in above-mentioned technical proposal is flushed with the upper end of the described first tubular interface, And the equal flanging in upper end of the filter wall is extremely bonded with the upper end of the described first tubular interface tube wall.

The beneficial effect of above-mentioned technical proposal is: structure is simple.

Further, filter housing described in above-mentioned technical proposal be spherical or its longitudinal section be tubaeform and butt end upward.

The beneficial effect of above-mentioned technical proposal is: structure is simple, and convenient for filtered fluid flowed directly into filter housing to In centrifuge tube.

Further, the first tubular lower end of interface described in above-mentioned technical proposal is extended down in the filter housing in downwards.

The beneficial effect of above-mentioned technical proposal is: so avoidable filtered fluid is flowed down along filter housing inner wall and is filled by negative pressure It sets and siphons away.

The two of the purpose of this utility model are to provide a kind of cell filtration system, including negative pressure device, pipe cap and at least Cell filtration component described in one；

When the cell filtration component is one, it is tubular that any one of third is removably arranged in the pipe cap Interface is simultaneously blocked, and a remaining tubular interface of third is connected to the air inlet of the negative pressure device；

When the cell filtration component is multiple, the tubular interface of third of multiple cell filtration components is passed sequentially through Plastic flexible pipe sequential communication, any one in the tubular interface of remaining two thirds are connected to the air inlet of the negative pressure device, The pipe cap is arranged in another tubular interface of third, and is blocked.

The beneficial effect of above-mentioned technical proposal is: its structure is simple, can an independent cell filtration component be filtered, Can also multiple cell filtration components filter multiple samples simultaneously, and while being multiple, also need to only match a pipe cap and a negative pressure dress It sets.

Further, negative pressure device described in above-mentioned technical proposal is syringe.

The beneficial effect of above-mentioned technical proposal is: it is easy to use, and can be adjusted according to the actual situation by operator Negative pressure amount.

Detailed description of the invention

Fig. 1 is the structure diagram of cell filtration component described in the utility model embodiment 1；

Fig. 2 is the structure diagram of filter described in the utility model embodiment 1；

Fig. 3 is the structure diagram of filter housing described in the utility model embodiment 1；

Fig. 4 is another structure diagram of filter housing described in the utility model embodiment 1；

Fig. 5 is the structure diagram of filtration system described in the utility model embodiment 2.

In figure: 1 cell filtration component, 11 filters, 12 filter housings, 121 first tubular interfaces, 122 second tubular interfaces, The tubular interface of 123 thirds, 13 centrifuge tubes, 2 negative pressure devices, 3 pipe caps.

Specific embodiment

Embodiment 1

The principles of the present invention and feature are described below in conjunction with attached drawing, example is served only for explaining that this is practical It is novel, it is not intended to limit the scope of the utility model.

As shown in Figure 1, Figure 2 and Figure 3, a kind of cell filtration component, including filter 11, filter housing are present embodiments provided 12 and centrifuge tube 13, the filter 11 is one and is vertically arranged and opening up groove body, the slot bottom cloth of the filter 11 Full sieve pore；The filter housing 12 is vertically arranged and inner hollow shell, and the upper end of the filter housing 12 is equipped with to be connected with inside it The first tubular interface 121 that is logical and being matched with the filter 11, lower end be equipped with its it is internal be connected to and with the centrifugation The second tubular interface 122 that 13 nozzle of pipe matches, set on side wall there are two with its internal tubular interface of third being connected to 123, the lower end of the filter 11 is extend into from top to bottom in the described first tubular interface 121, and is tightly connected with it, described Centrifuge tube 13 is vertically arranged in the lower section of the filter housing 12, the second tubular interface 122 extend into from top to bottom it is described from It in the nozzle of heart pipe 13, and is tightly connected with it, structure is simple, filter is mounted in the first tubular interface, by sample It is added in filter and filters, filtered fluid is added dropwise in filter housing and is flowed into centrifuge tube through the second tubular interface, wherein two One in a tubular interface of third can block, the external negative pressure equipment of the tubular interface of another third, in this way, in filter housing and It is at negative pressure state at centrifuge tube, is conducive to the rate of filtration of the filtered fluid in accelofilter.

As shown in figure 4, further, the upper end of filter 11 described in above-mentioned technical proposal and the described first tubular interface Upper end flush, and the equal flanging in upper end of 11 side wall of the filter is to upper end with the described first tubular 121 tube wall of interface Fitting.

And the filter, when being inlaid in the first tubular interface, lower end can stretch in filter housing, so that Filter can accommodate more solution.

Further, filter housing 12 described in above-mentioned technical proposal be spherical or its longitudinal section be tubaeform and butt end upward (such as inverted truncated cone-shaped), structure is simple, and flows directly into filter housing to centrifuge tube convenient for filtered fluid.

Further, the first tubular 121 lower end of interface described in above-mentioned technical proposal is extended down to the filter housing 12 in downwards Interior, so avoidable filtered fluid is flowed down along filter housing inner wall and is siphoned away by negative pressure device.

Wherein, the bottom wall of the filter can use metallic sieve, and its side wall adopts made of plastic, same filter housing It is that plastic material is made with centrifuge tube, so convenient for carrying out sterilization to it, is also convenient for reusing.

Preferably, two tubular interfaces of third are separately positioned on the upper end of the filtering shell side wall, it is additionally preferred to, The first tubular interface and the second tubular interface coaxial arrangement.

Preferably, the filter is preferably cylinder, and corresponding first tubular interface is to cooperate tubular with it Interface, and the outer diameter of filter is equal to the internal diameter of the first tubular interface, and the lower end of the filter is inserted into the from top to bottom In one tubular interface, make the two co-axial seal connection, certainly under external force, filter can also be pulled out out of first tubular interface Out, there is only led to the filtering being connected in filter and filter housing by the sieve pore on filter bottom wall after the two is tightly connected Road.

And the connection of the second tubular interface and centrifuge tube nozzle directlys adopt the mode of grafting, i.e., under the second tubular interface End is directly inserted into centrifuge tube and connect with its ferrule, i.e., the outer diameter of the second tubular interface is equal to the interior of centrifuge tube nozzle Diameter.

Embodiment 2

As shown in figure 5, the two of the purpose of this utility model are to provide a kind of cell filtration system, including negative pressure device 2, Pipe cap 3 and at least one cell filtration component 1 as described in Example 1, the negative pressure device 2 and pipe cap 3 are one；

When only needing to handle a sample, the cell filtration component 1 is one at this time, and the pipe cap 3 is dismountable Be arranged and at the tubular interface 123 of any one of third and blocked, a remaining tubular interface 123 of third with The air inlet of the negative pressure device 2 is connected to；

Usual experimenter needs the multiple samples of single treatment, at this point, for convenience, usually carrying out simultaneously to multiple samples Filtering, the cell filtration component 1 is multiple at this time, and the tubular interface 123 of third of multiple cell filtration components 1 is successively It is remaining by plastic flexible pipe sequential communication (mode of communicating of the tubular interface of third of multiple filter housings is with specific reference to Fig. 5) The tubular interface 123 of two thirds in any one be connected to the air inlet of the negative pressure device 2, the pipe cap 3 is arranged another At one tubular interface 123 of third, and blocked, structure is simple, can an independent cell filtration component carried out Filter, can also multiple cell filtration components filter multiple samples simultaneously, and while being multiple, also need to only match a pipe cap and one is negative Pressure device.

Further, negative pressure device 2 described in above-mentioned technical proposal is syringe, easy to use, while convenient for sterilizing, And negative pressure amount can be adjusted by operator according to the actual situation.Using syringe.

Certainly when in use, the pipe cap and to by the modeling of the tubular orifice of the third of two neighboring filter housing Material hose and the pipeline of syringe connection are also required to sterilize, wherein plastic flexible pipe is to need to infuse with third orifice Its junction of anticipating is unable to gas leakage, and pipeline generallys use transparent hose, and wherein pipe cap is groove body shape open at one end, is directly arranged It is blocked in the end of the corresponding tubular interface of third.

The above is only the preferred embodiment of the present invention, is not intended to limit the utility model, all practical at this Within novel spirit and principle, any modification, equivalent replacement, improvement and so on should be included in the guarantor of the utility model Within the scope of shield.

Claims (6)

1. a kind of cell filtration component, which is characterized in that including filter (11), filter housing (12) and centrifuge tube (13)；It is described Filter housing (12) is inner hollow shell, the upper end of the filter housing (12) be equipped with its it is internal be connected to and with the filter (11) the tubular interface of first matched (121), lower end be equipped with its it is internal be connected to and with the centrifuge tube (13) nozzle phase Second tubular interface (122) of cooperation, set on side wall there are two with its internal tubular interface of third (123) being connected to, it is described Filter (11) is sealingly mounted at the described first tubular interface (121), and the centrifuge tube (13) is vertically arranged in the filtering The lower section of shell (12), the second tubular interface (122) extend into the nozzle of the centrifuge tube (13), and with its company of sealing It connects.

2. cell filtration component according to claim 1, which is characterized in that the upper end of the filter (11) and described the The upper end of one tubular interface flushes, and the equal flanging in upper end of the filter (11) side wall to the described first tubular interface (121) the upper end fitting of tube wall.

3. cell filtration component according to claim 2, which is characterized in that the filter housing (12) is spherical or it is indulged and cuts Face be tubaeform and butt end upward.

4. cell filtration component according to any one of claim 1-3, which is characterized in that the first tubular interface (121) lower end is extended down in the filter housing (12) in downwards.

5. a kind of cell filtration system, which is characterized in that including negative pressure device (2), pipe cap (3) and at least one such as claim The described in any item cell filtration components (1) of 1-4；

When the cell filtration component (1) is one, the pipe cap (3) is removably arranged in any one of third pipe At shape interface (123) and blocked, a remaining tubular interface of third (123) and the negative pressure device (2) into Port connection；

When the cell filtration component (1) is multiple, the tubular interface of third (123) of multiple cell filtration components (1) Pass sequentially through plastic flexible pipe sequential communication, any one in the tubular interface of remaining two thirds (123) and the negative pressure device (2) air inlet connection, the pipe cap (3) is arranged at another described tubular interface of third (123), and is blocked.

6. cell filtration system according to claim 5, which is characterized in that the negative pressure device (2) is syringe.