CN218444630U - Cell solution sampler and sampling device - Google Patents

Abstract

The utility model belongs to the technical field of medical instrument, especially, relate to a cell solution sampler and sampling equipment. The cell solution sampler comprises a first joint, a pipe clamp, a sampling container, a second joint and a filter, wherein one end of the first joint is used for connecting an external cell container, the other end of the first joint is communicated with the sampling container, one end of the second joint is connected with the filter, and the other end of the second joint is communicated with one end, far away from the first joint, of the sampling container; one end of the filter, which is far away from the second connector, is used for an external liquid extractor; the tube clamp is clamped on the pipeline between the first connector and the sampling container. The utility model discloses in, this cell solution sampler's simple structure, low in manufacturing cost, convenient to use.

Description

Cell solution sampler and sampling device

Technical Field

The utility model belongs to the technical field of medical instrument, especially, relate to a cell solution sampler and sampling equipment.

Background

With the continuous progress of medical technology, the technology of treating patients with cell solutions is also continuously applied to various hospitals. After the cell solution is cultured in the cell container, the cell solution needs to be transferred to another container for processing, for example, to a centrifuge tube for centrifugation or to a freezing tube for freezing and storing. In addition, in the production process of cell drugs, the cell solution in the cell container needs to be sampled periodically to monitor the indicators of cell activity, cell loss rate, cell density, etc. In order to ensure the interference of the external environment to the cell solution, when the cell solution is sampled, it is necessary to ensure that the cell solution is sampled in a sterile environment.

In the prior art, an experimenter generally moves a cell sampling container to a sterile laboratory, then opens a cover of the cell sampling container, then uses a dropper and the like to suck cell liquid in the cell sampling container, then places the cell liquid sucked in the dropper into the cell sampling container, and finally covers the cover of the cell sampling container; the method of sampling a cell solution using a cell sampler requires frequent opening of a lid of a cell sampling container and frequent use of a pipette, and has problems of complicated structure and inconvenience in use.

SUMMERY OF THE UTILITY MODEL

The utility model discloses the technical problem that will solve is: aiming at the problems of complex structure and inconvenient use of the cell sampler in the prior art, a cell solution sampler and sampling equipment are provided.

In order to solve the above technical problem, an embodiment of the present invention provides a cell solution sampler, including a first joint, a pipe clamp, a sampling container, a second joint and a filter, wherein one end of the first joint is used for connecting an external cell container, the other end of the first joint is communicated with the sampling container, one end of the second joint is connected with the filter, and the other end of the second joint is communicated with one end of the sampling container far from the first joint; one end of the filter, which is far away from the second connector, is used for an external liquid extractor; the tube clamp is clamped on the pipeline between the first connector and the sampling container.

Optionally, the sampling container is provided with a graduation mark for marking the volume of the cell solution in the sampling container.

Optionally, the sampling vessel comprises a plurality of dosing vials connected in series.

Optionally, the filter comprises a mounting shell provided with a through hole, and a cell-proof solution filtering membrane and a sterilization filtering membrane which are mounted in the mounting shell at intervals, one end of the mounting shell is connected with one end of the second connector far away from the sampling container, the other end of the mounting shell is used for connecting an external liquid extractor, and the external liquid extractor is communicated with the sampling container through the through hole; the anti-cellular solution filtering membrane is located between the sterilization filtering membrane and the sampling vessel.

Optionally, the cell solution sampler further comprises a first protective cap and a second protective cap, the first protective cap is detachably covered on one end of the first joint far away from the sampling container, and the second protective cap is detachably covered on one end of the filter far away from the second joint.

Optionally, a first connecting thread is arranged on the first joint, a second connecting thread is arranged on the first protective cap, and the first protective cap is covered on the first joint through the first connecting thread and the second connecting thread which are in threaded connection.

Optionally, a third connecting thread is arranged on the second joint, a fourth connecting thread is arranged on the second protective cap, and the second protective cap is covered on the filter through the third connecting thread and the fourth connecting thread which are in threaded connection.

Optionally, the cell solution sampler further comprises a tube connector comprising a main interface and at least two branch interfaces each in communication with the main interface; the first connector is communicated with one end, away from the branch interface, of the main interface, one end, away from the main interface, of the branch interface is communicated with branch pipelines, each branch pipeline is internally provided with the sampling container and the second connector which are communicated in sequence, and the pipe clamp is arranged on the branch pipeline between the sampling container and the branch interface.

The utility model discloses in, outside liquid suction ware extracts this cell solution sampler for negative pressure state, and the cell solution sampler of negative pressure state extracts the sample container with the cell liquid in the outside cell container, works as when the volume of the cell solution among the sample container reaches the volume of predetermineeing (also, the volume of the cell solution that needs the sample), outside liquid suction ware stops the sample. Clamping a pipeline between the sampling container and the first joint by using the pipe clamp, and sealing the pipeline by using processes such as heat sealing and the like so as to separate the first joint from the sampling container; the separated sampling container is communicated with the second joint, and the sampling container is in a closed state. Then, the tube at the end of the sampling container remote from the second connector is cut open and the mouth of the tube is directed towards an external vessel (centrifuge cup, culture dish, test tube, etc.), opening the tube clamp. Finally, injecting gas into the sampling container by using an external liquid extractor, wherein the gas enables the cell solution in the sampling container to flow into an external vessel, so that the sampling work of the cell solution is completed; and when the external liquid extractor fills gas into the sampling container, the gas is filtered by the filter, so that the pollution of the gas to the cell solution is avoided, and the cleanness of the cell solution is ensured. The utility model discloses in, this cell solution sampler's simple structure, low in manufacturing cost, convenient to use.

The utility model also provides a sampling device, including liquid suction ware and foretell cell solution sampler.

Optionally, the sampling device further comprises a heat sealer for heat sealing the tubing between the first connector and the sampling container.

Drawings

The present invention will be further explained with reference to the drawings and examples.

Fig. 1 is a schematic structural diagram of a cell solution sampler according to an embodiment of the present invention;

fig. 2 is a schematic structural diagram of a cell solution sampler according to another embodiment of the present invention.

The reference numerals in the specification are as follows:

1. a first joint; 2. a pipe clamp; 3. a sampling container; 4. a second joint; 5. a filter; 6. a first protective cap; 7. a second protective cap; 8. a pipe joint; 81. a main interface; 82. and (4) supporting an interface.

Detailed Description

In order to make the technical problems, technical solutions and advantageous effects of the present invention more clearly understood, the following description is given in conjunction with the accompanying drawings and embodiments to illustrate the present invention in further detail. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the invention.

It is to be understood that the terms "upper", "lower", "left", "right", "front", "rear", "middle", and the like, indicate orientations or positional relationships that are based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and to simplify the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and therefore, are not to be construed as limiting the invention.

As shown in fig. 1, a cell solution sampler provided by an embodiment of the present invention includes a first joint 1, a pipe clamp 2, a sampling container 3, a second joint 4 and a filter 5, wherein one end of the first joint 1 is used for connecting an external cell container, the other end of the first joint 1 is communicated with the sampling container 3, one end of the second joint 4 is connected with the filter 5, and the other end of the second joint 4 is communicated with one end of the sampling container 3 away from the first joint 1; the end of the filter 5 far away from the second connector 4 is used for an external liquid extractor; the pipe clamp 2 is clamped on the pipeline between the first connector 1 and the sampling container 3. It is understood that the sampling container 3 includes, but is not limited to, a dropping funnel, a hose, etc., an external cell container stores a cell solution therein, and an external liquid pump includes, but is not limited to, a syringe, a vacuum pump, etc.; and a pipeline (a hose) is arranged between the first connector 1 and the sampling container 3, and a pipeline is also arranged between the second connector 4 and the sampler.

Specifically, the external aspirator extracts the cell solution sampler in a negative pressure state, the cell solution sampler in the negative pressure state extracts the cell solution in the external cell container into the sampling container 3, and the external aspirator stops sampling when the volume of the cell solution in the sampling container 3 reaches a preset volume (i.e., the volume of the cell solution to be sampled). Clamping a pipeline between the sampling container 3 and the first connector 1 by using the pipe clamp 2, and sealing the pipeline by using processes such as heat sealing and the like so as to separate the first connector 1 from the sampling container 3; the separated sampling vessel 3 is communicated with the second joint 4, and the sampling vessel 3 is in a closed state. Then, the tube of the end of the sampling container 3 remote from the second connector 4 is cut open and the mouth of the tube is directed towards an external vessel (centrifuge cup, culture dish, test tube, etc.), and the tube clamp 2 is opened. Finally, injecting gas into the sampling container 3 by using an external liquid extractor, wherein the gas enables the cell solution in the sampling container 3 to flow into an external vessel, thereby completing the sampling work of the cell solution; and when the external liquid extractor fills gas into the sampling container 3, the gas is filtered by the filter, thereby avoiding the pollution of the gas to the cell solution and ensuring the cleanness of the cell solution. The utility model discloses in, this cell solution sampler's simple structure, low in manufacturing cost, convenient to use.

In one embodiment, the sampling container 3 is provided with a scale (not shown) for marking the volume of the cell solution in the sampling container 3. It can be understood that the graduation mark can be marked on the outer wall of the sampling container 3, and the user can clearly know the volume of the cell solution entering the sampling container 3 through the graduation mark, thereby avoiding the accident that the cell solution is excessively extracted and wasted, and the accident that the sample amount is insufficient due to the excessively-extracted cell solution.

In one embodiment, as shown in fig. 1, the sampling vessel 3 comprises a plurality of dosing vessels (not shown) connected in series. It is understood that the quantitative tubes are small-capacity containers, for example, the capacity of each quantitative tube can be set to 0.5ml, 1ml, 1.5ml or 5ml, etc., and the quantitative tubes can be designed to be spherical, cylindrical, etc. according to actual requirements; illustratively, the two quantitative tubes located at the outermost side communicate with the first connector 1 and the second connector 4. In this embodiment, the user progressively extracts the cell sap in the outside cell container to in the ration appearance pipe to the number that fills up the ration appearance pipe through cell solution obtains the sample volume of cell solution, thereby this cell solution sampler user of being convenient for observes the sample volume of cell solution, has improved its user experience and has felt.

In one embodiment, as shown in fig. 1, the filter 5 includes a mounting shell having a through hole, and a cell-proof solution filtering membrane (not shown) and a sterilization filtering membrane (not shown) mounted in the mounting shell at intervals, one end of the mounting shell is connected to one end of the second connector 4 away from the sampling container 3, the other end of the mounting shell is used for connecting an external liquid extractor, and the external liquid extractor is communicated with the sampling container 3 through the through hole; the anti-cell solution filtration membrane is located between the sterilization filtration membrane and the sampling vessel. It is understood that the cell preventing solution filtering membrane is positioned at a side close to the sampling vessel 3 and the sterilizing filtering membrane is positioned at a side close to the external liquid suction unit, and the cell preventing solution filtering membrane and the sterilizing filtering membrane have a gap required to allow the cell preventing solution filtering membrane and the sterilizing filtering membrane not to be attached to each other when the cell liquid or gas flows through the through-hole. In this embodiment, the cell solution prevention filtering membrane can play a role in blocking a cell solution, so as to avoid an accident that the cell solution contacts the sterilization filter to cause the failure of the sterilization filter; the sterilization filter membrane prevents impurities in the gas from entering the sampling container 3 when the external liquid extractor 4 fills the gas into the sampling container 3, thereby preventing the gas from polluting the cell solution and ensuring the cleanness of the cell solution. The cell-preventing solution filtering membrane may be a leukocyte-removing filtering membrane, the leukocyte-removing filtering membrane may be capable of filtering leukocytes to prevent the leukocytes from flowing out of the through holes, and the material of the leukocyte-removing filtering membrane may be cellulose acetate fiber aggregate, and the material of the nonwoven leukocyte filtering material sterilization filtering membrane may be polytetrafluoroethylene.

In one embodiment, as shown in fig. 1, the cell solution sampler further comprises a first protective cap 6 and a second protective cap 7, wherein the first protective cap 6 is detachably covered on one end of the first connector 1 far away from the sampling container 3, and the second protective cap 7 is detachably covered on one end of the filter 5 far away from the second connector 4. It is understood that the first connector 1 includes, but is not limited to, a luer connector and the like, and the first protective cap 6 includes, but is not limited to, a luer protective cap and the like; the second connector 4 includes, but is not limited to, a female luer connector or the like; the first protective cap 6 covers the first connector 1, and the second protective cap 7 covers the filter 5, so that the tightness of the cell solution sampler is ensured; when it is necessary to sample a cell solution using the cell solution sampler, the first and second caps 6 and 7 are removed, the first connector 1 is connected to an external cell container, and the filter 5 is connected to an external liquid extractor.

In an embodiment, a first connection thread (not shown) is provided on the first joint 1, a second connection thread (not shown) is provided on the first protective cap 6, and the first protective cap 6 covers the first joint 1 through the first connection thread and the second connection thread which are connected through threads. It is understood that the first connection thread may be a first external thread provided on the first joint 1, and correspondingly, the second connection thread is a first internal threaded hole provided on the first cap 6; alternatively, the first connection thread may be a first female screw hole provided on the first joint 1, and correspondingly, the second connection thread may be a first male screw provided on the first cap 6. The first external thread is connected with the first internal thread hole, so that the first protective cap 6 covers the first joint 1. Further, the first connector 1 is connected with the interface of the external cell container through threads. In this embodiment, the first cap 6 and the first joint 1 are easily attached to and detached from each other.

In an embodiment, as shown in fig. 1, a third connection thread (not shown) is provided on the second joint 4, a fourth connection thread (not shown) is provided on the second protective cap 7, and the second protective cap 7 covers the filter 5 through the third connection thread and the fourth connection thread which are connected through a screw thread. It is understood that the third connecting thread may be a second external thread provided on the filter 5, and correspondingly, the fourth connecting thread is a second internal thread hole provided on the second cap 7; alternatively, the third connection thread may be a second internal thread hole provided on the filter 5, and correspondingly, the fourth connection thread may be a second external thread provided on the second cap 7. The second external screw is coupled to the second internal screw hole so that the second cap 7 is closed over the filter 5. Further, the filter 5 is connected with the interface of the external liquid extractor through a screw thread. In this embodiment, the second protective cap 7 and the filter 5 are easily attached and detached.

In one embodiment, as shown in fig. 2, the cell solution sampler further comprises a tube adapter 8, the tube adapter 8 comprising a main interface 81 and at least two branch interfaces 82 each communicating with the main interface 81; the first joint 1 is communicated with one end of the main interface 81 far away from the branch interface 82, one end of each branch interface 82 far away from the main interface 81 is communicated with the sampling container 3 and the second joint 4 in sequence, and the pipe clamp 2 is clamped on a branch pipeline between the sampling container 3 and the branch interface 82. It can be understood that the branch interfaces 82 can be set as two, three, four, etc. according to actual requirements, and the number of the branch pipelines is equal to the number of the branch interfaces 82; the pipe clamp 2 is clamped on each branch pipeline, and the filter 5 and the second protective cap 7 are arranged on each branch pipeline; further, one end of each branch pipeline, which is far away from the branch connector 82, is connected with an external liquid extractor.

Specifically, the sampling steps of the cell solution sampler in this embodiment are: the branch pipes are clamped and closed by the pipe clamps 2, only one branch pipe is in a conducting state, correspondingly, the external liquid extractor connected to the branch pipe in the conducting state extracts cell solution into the corresponding sampling container 3, and when the sampling container 3 extracts sufficient cell solution, the branch pipe storing the cell solution is clamped by the pipe clamps 2. The tube clamp 2 is used to open and conduct another branch line, and the cell solution is pumped into the corresponding sampling container 3 by the same method. When the cell solution is stored in the plurality of sampling containers 3 of the plurality of branch pipelines, the branch pipelines storing the cell solution are sealed and disassembled in a heat sealing mode and the like, so that the cell solution sampler can finish the sampling work of the plurality of sampling containers 3 on the cell solution at one time, the sampling efficiency of the cell solution is improved, and the sampling cost of the cell solution sampler is further reduced.

The present invention also provides a sampling device, which comprises a liquid extractor (not shown) and the above-mentioned cell solution sampler.

In one embodiment, the sampling device further comprises a heat sealer (not shown) for heat sealing the pipe between the first connector 1 and the sampling container 3. It will be understood that the heat sealer is used to heat and seal the line between the first connector 1 and the sampling container 3.

The above description is merely exemplary of the cell solution sampler and sampling device of the present invention and is not intended to limit the present invention, and any modifications, equivalents, improvements, etc. made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.

Claims (10)

1. A cell solution sampler is characterized by comprising a first joint, a pipe clamp, a sampling container, a second joint and a filter, wherein one end of the first joint is used for connecting an external cell container, the other end of the first joint is communicated with the sampling container, one end of the second joint is connected with the filter, and the other end of the second joint is communicated with one end of the sampling container, which is far away from the first joint; one end of the filter, which is far away from the second connector, is used for an external liquid extractor; the tube clamp is clamped on the pipeline between the first connector and the sampling container.

2. The cell solution sampler of claim 1, wherein the sampling container is provided with a graduation mark for marking the volume of the cell solution in the sampling container.

3. The cell solution sampler of claim 1, wherein the sampling container comprises a plurality of quantitative tubes connected in series.

4. The cell solution sampler according to claim 1, wherein the filter comprises a mounting shell provided with a through hole, and a cell solution prevention filtering membrane and a sterilization filtering membrane which are mounted in the mounting shell at intervals, one end of the mounting shell is connected with one end of the second connector far away from the sampling container, the other end of the mounting shell is used for connecting an external liquid extractor, and the external liquid extractor is communicated with the sampling container through the through hole; the anti-cellular solution filtering membrane is located between the sterilization filtering membrane and the sampling vessel.

5. The cell solution sampler of claim 1, further comprising a first cap removably covering an end of the first connector distal from the sampling vessel and a second cap removably covering an end of the filter distal from the first connector.

6. The cell solution sampler according to claim 5, wherein the first joint is provided with a first connecting thread, the first protective cap is provided with a second connecting thread, and the first protective cap is covered on the first joint through the first connecting thread and the second connecting thread which are connected through screw threads.

7. The cell solution sampler according to claim 5, wherein the second connector is provided with a third connecting thread, the second cap is provided with a fourth connecting thread, and the second cap is covered on the filter through the third connecting thread and the fourth connecting thread which are connected by screw threads.

8. The cell solution sampler of claim 1, further comprising a tube adapter comprising a main port and at least two branch ports each in communication with the main port; the first connector is communicated with one end, away from the branch connector, of the main connector, one end, away from the main connector, of each branch connector is communicated with a branch pipeline, the branch pipelines are sequentially communicated with the sampling container and the second connector, and the pipe clamps are clamped on the branch pipelines between the sampling container and the branch connectors.

9. A sampling device comprising an aspirator and a cell solution sampler according to any one of claims 1 to 8.

10. The sampling device of claim 9, further comprising a heat sealer for heat sealing the tubing between the first connector and the sampling container.

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