CN208943510U - Column chromatography uses experimental provision - Google Patents
Column chromatography uses experimental provision Download PDFInfo
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- CN208943510U CN208943510U CN201821364072.4U CN201821364072U CN208943510U CN 208943510 U CN208943510 U CN 208943510U CN 201821364072 U CN201821364072 U CN 201821364072U CN 208943510 U CN208943510 U CN 208943510U
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- state
- syringe
- experimental provision
- column
- detachably connected
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Abstract
The utility model provides a kind of column chromatography experimental provision, it include: chromatographic column, valve, the first syringe, the second syringe and third syringe, experimental provision has first state, the second state and the third state, and when being in first state, the first connection end is connected to connecting pin；When in the second state, the second connection end is connected to connecting pin；When in the third state, third connecting end is connected to connecting pin.The technical solution of the utility model overcomes the problems, such as that radiated time suffered by more complicated, laboratory technician is too long using the experimentation of chromatography column separating purification in the prior art.
Description
Technical field
The utility model relates to use for laboratory instrument and equipment technical fields, and in particular to a kind of column chromatography uses experimental provision.
Background technique
Column chromatography is common isolation and purification method in chemistry, and first separation needs replacing mobile phase, eluent and elution
Liquid.When radioactive compound label, in order to reduce radiation of the radioactive compound to laboratory technician, need to contract as much as possible
The short label time, and laboratory technician is made to carry out radioactive label manually in the label case protected with lead glass.Labeled compound
The small column separating purification of C18 is generally used, usual experimenter requires to open case when replacing mobile phase, eluent, leacheate
Then body replaces different syringes again, this experiment method has the following disadvantages: that operating process is cumbersome, replaces syringe institute
With overlong time, and then make to test overlong time used, finally keeps radiated time suffered by laboratory technician too long.
Therefore, present urgent problem to be solved is how to simplify operating process, so as to reduce the raying of laboratory technician institute.
Utility model content
The main purpose of the utility model is to provide a kind of column chromatography experimental provisions, to solve to utilize in the prior art
The problem that the experimentation of chromatography column separating purification is more complicated, radiated time suffered by laboratory technician is too long.
To achieve the above object, the utility model provides a kind of column chromatography experimental provision, comprising: chromatographic column, valve,
First syringe, the second syringe and third syringe, valve have the connecting pin and connecting pin being detachably connected with chromatographic column
The first connection end being oppositely arranged, the second connection being arranged between connecting pin and the first connection end and are connected to end phase with second at end
To the third connecting end of setting；First syringe is connected to end with first and is detachably connected；Second syringe is connected to end with second can
Dismantling connection；Third syringe is detachably connected with third connecting end；Wherein, experimental provision has first state, the second state
And the third state, when being in first state, the first connection end is connected to connecting pin；When in the second state, second connection end with
Connecting pin connection；When in the third state, third connecting end is connected to connecting pin.
Further, column chromatography experimental provision further include: the first connection of the first syringe of connection and the first connection end
Pipe, connects the second connecting tube of the second syringe and the second connection end, and the third for connecting third syringe and third connecting end connects
Adapter tube.
Further, column chromatography experimental provision further include: adapter one end is detachably connected with chromatographic column, one end and company
End is connect to be detachably connected.
Further, adapter includes accommodating cavity and takes shape in accommodating cavity one end, is connected to accommodating cavity, being used for and chromatography
The connecting column of column connection, an end face of the separate connecting column of accommodating cavity are equipped with through-hole, and connection end is detachably connected with through-hole.
Further, accommodating cavity approximation is in platform body structure, and the direction of accommodating cavity, the cross section of accommodating cavity are flowed through along liquid
Area is gradually reduced.
Further, the diameter of connecting column is less than the diameter of chromatographic column.
Further, valve is four way plug valve, manipulates four way plug valve so that experimental provision is in first state, second
State or the third state.
Further, the first connecting tube is detachably connected with the first syringe；Second connecting tube and the second syringe are removable
Unload connection；Third connecting tube is detachably connected with third syringe.
Technical solutions of the utility model have the advantages that during actual experiment have in advance in each syringe
Respective liquid simultaneously with corresponding connection end connect, then further according to specific experiment need, control experimental provision be in first state,
Second state or the third state, such as: manipulation experimental provision is in first state, pushes the first syringe, so that the first injection
Liquid in device flows into connecting pin from the first connection end, and finally flows into chromatographic column；Then experimental provision is made to be in second again
State pushes the liquid in the second syringe to be flowed into chromatographic column, likewise, finally experimental provision is made to be in third shape again
State, then the liquid in third syringe is pushed into chromatographic column.When isolate and purify experiment using this experimental provision not
Needing to replace different syringes back and forth, experimental implementation process is simpler, and it is shorter the time required to experiment, therefore can also reduce
The raying of laboratory technician institute.
Detailed description of the invention
It, below will be right in order to illustrate more clearly of specific embodiment of the present invention or technical solution in the prior art
Specific embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, it is described below
In attached drawing be that some embodiments of the utility model are not paying creativeness for those of ordinary skill in the art
Under the premise of labour, it is also possible to obtain other drawings based on these drawings.In the accompanying drawings:
Fig. 1 shows the overall structure diagram of the column chromatography experimental provision of the utility model.
Wherein, the appended drawing reference in above-mentioned attached drawing are as follows:
10, chromatographic column；20, valve；21, connecting pin；22, the first connection end；23, the second connection end；24, third connecting
End；31, the first syringe；32, the second syringe；33, third syringe；41, the first connecting tube；42, the second connecting tube；43,
Third connecting tube；50, adapter；51, connecting column.
Specific embodiment
The technical solution of the utility model is clearly and completely described below in conjunction with attached drawing, it is clear that described
Embodiment is the utility model a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, originally
Field those of ordinary skill every other embodiment obtained without making creative work belongs to practical
Novel protected range.
A kind of column chromatography experimental provision as shown in Figure 1, comprising: chromatographic column 10, valve 20, the first syringe 31, the
Two syringes 32 and third syringe 33, valve 20 have the connecting pin 21 being detachably connected with chromatographic column 10 and connecting pin 21
The first connection end 22 for being oppositely arranged, the second connection end 23 being arranged between connecting pin 21 and the first connection end 22 and with second
The third connecting end 24 that connection end 23 is oppositely arranged；First syringe 31 is connected to end 22 with first and is detachably connected；Second injection
Device 32 is connected to end 23 with second and is detachably connected；Third syringe 33 is detachably connected with third connecting end 24；Wherein, experiment dress
It sets with first state, the second state and the third state, when being in first state, the first connection end 22 is connected to connecting pin 21；
When in the second state, the second connection end 23 is connected to connecting pin 21；When in the third state, third connecting end 24 and connecting pin
21 connections.
There is respective liquid in advance during actual experiment, in each syringe and connected with corresponding connection end, so
It being needed afterwards further according to specific experiment, control experimental provision is in first state, the second state or the third state, such as: manipulation is real
Experiment device is in first state, pushes the first syringe 31, so that the liquid in the first syringe 31 is flowed from the first connection end 22
Enter connecting pin 21, and finally flows into chromatographic column 10；Then so that experimental provision is in the second state again, push the second syringe 32
Interior liquid is flowed into chromatographic column 10, likewise, finally experimental provision is made to be in the third state again, then by third syringe 33
Interior liquid is pushed into chromatographic column 10.It does not need to replace difference back and forth when carrying out using this experimental provision and isolate and purify experiment
Syringe, experimental implementation process is simpler, shorter the time required to experiment, therefore can also reduce the raying of laboratory technician institute.
Specifically, column chromatography experimental provision further include: the first syringe 31 of connection is connected to the first of end 22 with first and connects
Adapter tube 41, the second connecting tube 42 that the second syringe 32 of connection is connected to end 23 with second, connects third syringe 33 and third connects
The third connecting tube 43 of go side 24.First connecting tube 41, the second connecting tube 42 and third connecting tube 43 are set and extend each company
The length of go side, and then be conducive to connect each syringe with corresponding connection end.
Specifically, column chromatography experimental provision further include: adapter 50, one end are detachably connected with chromatographic column 10, one end
It is detachably connected with connecting pin 21.Setting adapter 50 links together convenient for chromatographic column 10 with valve 20, adapter 50 and company
Connecing end 21, removably connection is conveniently replaceable different chromatographic columns 10.
Specifically, adapter 50 includes accommodating cavity and takes shape in accommodating cavity one end, is connected to accommodating cavity, being used for and chromatography
One end face of the connecting column 51 that column 10 connects, the separate connecting column 51 of accommodating cavity is equipped with through-hole, is connected to end and through-hole is detachable
Connection.Setting connecting column 51 and through-hole are conducive to chromatographic column 10 and link together with valve 20.
Specifically, accommodating cavity approximation is in platform body structure, and the direction of accommodating cavity, the cross section face of accommodating cavity are flowed through along liquid
Product is gradually reduced.Setting accommodating cavity flows in chromatographic column 10 convenient for liquid.
Specifically, the diameter of connecting column 51 is less than the diameter of chromatographic column 10.Setting connecting column 51 in this way is placed in chromatographic column 10
It is interior, so that connecting column 51 links together with chromatographic column 10.
Specifically, valve 20 is four way plug valve, manipulates four way plug valve so that experimental provision is in first state, second
State or the third state.Certainly, valve 20 can also be other valves 20 that can be realized switching flow channel for liquids function.
Specifically, the first connecting tube 41 is detachably connected with the first syringe 31；Second connecting tube 42 and the second syringe
32 are detachably connected；Third connecting tube 43 is detachably connected with third syringe 33.Dismountable connection type is conveniently replaceable respectively
A syringe.
Specifically, chromatographic column 10 is C18 column.
Specific experiment process is described below:
When specific operation, adapter 50 is connected on C18 column, by completely reacted the first injection of label mixed solution deposit
In device 31, switching four way plug valve is at first state, then label mixed solution is pushed into C18 column, keeps crude product rich
Collection is on C18 column cylinder filler；Then switching four way plug valve is at the second state, then will take out in advance in the second syringe 32
Good 20ml pure water is pushed on C18 column, carries out injection washing to C18 column, removes extra radioactivity heteroion, and waste liquid enters
Waste liquid bottle；After cleaning, switching four way plug valve is at the third state, with what is smoked in advance in third syringe 33
To set product bottle stand-by by the product elution for adsorbing on a cl 8 column for 500ul leacheate.Compared with original experimentation, with this reality
After experiment device, whole process does not need replacement syringe, it is only necessary to be at different conditions by switching four way plug valve, just
Upper prop, cleaning, elution operation quickly can be easily carried out, operating process is greatly simplified, shortens experimental period, reduce experimenter
Radiation injury.
Obviously, the above embodiments are merely examples for clarifying the description, and does not limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And it is extended from this it is obvious variation or
It changes among the protection scope created still in the utility model.
Claims (8)
1. a kind of column chromatography experimental provision characterized by comprising
Chromatographic column (10)；
Valve (20), comprising: the connecting pin (21) being detachably connected with the chromatographic column (10) and the connecting pin (21) are opposite
Second connection end of the first connection end (22), setting being arranged between the connecting pin (21) and first connection end (22)
(23) and with described second it is connected to the third connecting end (24) that end (23) is oppositely arranged；
First syringe (31) is connected to end (22) with described first and is detachably connected；
Second syringe (32) is connected to end (23) with described second and is detachably connected；
Third syringe (33) is detachably connected with the third connecting end (24)；
Wherein, the experimental provision has first state, the second state and the third state, described when being in the first state
First connection end (22) is connected to the connecting pin (21)；When in second state, second connection end (23) and institute
State connecting pin (21) connection；When in the third state, the third connecting end (24) is connected to the connecting pin (21).
2. column chromatography experimental provision according to claim 1, which is characterized in that further include: connection first injection
The first connecting tube (41) that device (31) is connected to end (22) with described first connects second syringe (32) and described second and connects
The second connecting tube (42) of go side (23) connects the third syringe (33) and connects with the third of the third connecting end (24)
It manages (43).
3. column according to claim 1 chromatography experimental provision, which is characterized in that further include: adapter (50), one end with
The chromatographic column (10) is detachably connected, and one end is detachably connected with the connecting pin (21).
4. column chromatography experimental provision according to claim 3, which is characterized in that the adapter (50) includes accommodating
Chamber and the connecting column for taking shape in described accommodating cavity one end, being connected to the accommodating cavity, being used to connect with the chromatographic column (10)
(51), the end face far from the connecting column (51) of the accommodating cavity is equipped with through-hole, and the connection end and the through-hole can
Dismantling connection.
5. column chromatography experimental provision according to claim 4, which is characterized in that the accommodating cavity approximation is in stage body knot
Structure, the direction of accommodating cavity is flowed through along liquid, and the cross-sectional area of the accommodating cavity is gradually reduced.
6. column chromatography experimental provision according to claim 4, which is characterized in that the diameter of the connecting column (51) is less than
The diameter of the chromatographic column (10).
7. column chromatography experimental provision according to claim 1, the valve (20) is four way plug valve, manipulation described four
Lead to plug valve so that the experimental provision is in the first state, second state or the third state.
8. column chromatography experimental provision according to claim 2, first connecting tube (41) and first syringe
(31) it is detachably connected；Second connecting tube (42) is detachably connected with second syringe (32)；The third connection
Pipe (43) is detachably connected with the third syringe (33).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821364072.4U CN208943510U (en) | 2018-08-22 | 2018-08-22 | Column chromatography uses experimental provision |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821364072.4U CN208943510U (en) | 2018-08-22 | 2018-08-22 | Column chromatography uses experimental provision |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208943510U true CN208943510U (en) | 2019-06-07 |
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ID=66735489
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821364072.4U Expired - Fee Related CN208943510U (en) | 2018-08-22 | 2018-08-22 | Column chromatography uses experimental provision |
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| Country | Link |
|---|---|
| CN (1) | CN208943510U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110665257A (en) * | 2019-11-07 | 2020-01-10 | 青海省药品检验检测院 | Chromatography device for experiments |
-
2018
- 2018-08-22 CN CN201821364072.4U patent/CN208943510U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110665257A (en) * | 2019-11-07 | 2020-01-10 | 青海省药品检验检测院 | Chromatography device for experiments |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190607 Termination date: 20200822 |