CN204910866U - Be used for purified chromatographic system of high -purity ganglioside - Google Patents
Be used for purified chromatographic system of high -purity ganglioside Download PDFInfo
- Publication number
- CN204910866U CN204910866U CN201520712333.7U CN201520712333U CN204910866U CN 204910866 U CN204910866 U CN 204910866U CN 201520712333 U CN201520712333 U CN 201520712333U CN 204910866 U CN204910866 U CN 204910866U
- Authority
- CN
- China
- Prior art keywords
- chromatographic column
- purifying
- solid layer
- constant flow
- flow pump
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The utility model relates to a be used for purified chromatographic system of high -purity ganglioside, including into kind liquid storage device, chromatography column and play appearance liquid storage device, being equipped with the first solid layer and the second solid layer of the coupling of establishing ties in proper order in the chromatography column is packed with filling by silica gel and forms in first solid layer, is packed with filling by the gel and form in second solid layer, advancing a kind liquid storage device and including sample liquid holding vessel, first eluate holding vessel and first constant flow pump, sample liquid holding vessel and first eluate holding vessel pass through the solenoid valve to be connected with the one end of first constant flow pump, and the other end of first constant flow pump is connected with the entrance of chromatography column. This system's purification operation is simple more, quick, high -efficient, reliable, and has the cost benefit, has extremely important using value.
Description
Technical field
The utility model relates to medicine portable device technical field, is specifically related to a kind of tomographic system for high-purity ganglioside purifying.
Background technology
The method for making of GM1 (Monosialotetrahexosylganglio-ideSodium, GM1) is generally divided into Isolation and purification two steps.Purification step needs crude product respectively through ion-exchange chromatography and reversed phase column chromatography, a kind of method for making of high purity monosialogangliosides sodium disclosed in the patent application document CN103524572A of applicant, carries out silica gel column chromatography and SourceRPC30 column chromatography successively by the aqueous phase collection liquid after extracting desalination.Although the operation that tradition carries out column chromatography respectively has good purification effect, level layer operations is more loaded down with trivial details, and cost is higher; For this reason, applicant have developed a kind of tomographic system for high-purity ganglioside purifying newly, makes purification process more simple, quick, efficient, reliable, and has cost benefit.
Utility model content
The purpose of this utility model is to overcome defect of the prior art, design a kind of tomographic system for high-purity ganglioside purifying, this system purification process is more simple, quick, efficient, reliable, and has cost benefit, has very important using value.
For achieving the above object, the technical scheme that the utility model adopts is a kind of tomographic system for high-purity ganglioside purifying, comprise sample introduction liquid storaging device, chromatographic column and go out sample liquid storaging device, the first solid layer and second solid layer of series coupled is successively provided with in described chromatographic column, described first solid layer is filled by silica filler and is formed, described second solid layer is filled by gel filler and is formed, and gel filler preferably but be not limited to SourceRPC30;
Described sample introduction liquid storaging device comprises sample liquid holding vessel, the first eluent holding vessel and the first constant flow pump, described sample liquid holding vessel is connected by magnetic valve one end with the first constant flow pump with the first eluent holding vessel, and the described other end of the first constant flow pump is connected with the porch of chromatographic column.。
Wherein preferred technical scheme is also provided with unhurried current layer between described first solid layer and the second solid layer, and the chromatographic column post jamb being positioned at described unhurried current layer is provided with the second eluent entrance; Described sample introduction liquid storaging device also comprises the second eluent storage tank and the second constant flow pump, and described second eluent storage tank is connected by magnetic valve one end with the second constant flow pump, and the other end of described second constant flow pump is connected with the second eluent entrance.
Wherein preferred technical scheme is provided with filter plate between described unhurried current layer and the second solid layer.
Wherein preferred technical scheme also has, and the upper end of described chromatographic column is provided with upper cover plate, and lower end is provided with lower cover.
Wherein preferred technical scheme also has, and is provided with upper filter screen, is provided with lower filter screen between described lower cover and chromatographic column between described upper cover plate and chromatographic column.
Wherein preferred technical scheme also has, described in go out sample liquid storage system and comprise waste liquid tank and qualified sample tank, described waste liquid tank is connected with the outlet of chromatographic column respectively by magnetic valve with qualified sample tank.
Wherein preferred technical scheme also has, described in go out sample liquid storage system and also comprise and intersect sample tank, described intersection sample tank is connected with the outlet of chromatographic column by magnetic valve.
Wherein preferred technical scheme also has, and the surface of described chromatographic column is provided with form.
Wherein preferred technical scheme also has, and the inside of described sample liquid holding vessel is provided with elution device.
Advantage of the present utility model and beneficial effect are:
1, the tomographic system of the application is by the composition chromatographic column that silica gel column chromatography and gel filtration chromatography combined, two step operating procedures of script purifying ganglioside are reduced to an operating procedure, shorten the chromatographic runs time, reduce the use of organic solvent, reduce costs.
2, this tomographic system passes through the increase at the middle unhurried current layer of two solid layers and the second eluent, optimize this purification step further, the second eluent (adding suitable eluent for gel column) can be added according to actual service condition, improve purification efficiency.
3, be provided with intersection sample tank, for collecting the liquid in washing process, containing a small amount of sample, then the liquid collected in intersection sample tank regulated, then just can carry out again the step of loading and chromatographic purifying.
Accompanying drawing explanation
Fig. 1 is the structural representation of embodiment 1 for the tomographic system of high-purity ganglioside purifying;
Fig. 2 is the sectional view of embodiment 1 for the chromatographic column of the tomographic system of high-purity ganglioside purifying;
Fig. 3 is the structural representation of embodiment 2 for the tomographic system of high-purity ganglioside purifying;
Fig. 4 is the sectional view of embodiment 2 for the chromatographic column of the tomographic system of high-purity ganglioside purifying.
Detailed description of the invention
Below in conjunction with drawings and Examples, detailed description of the invention of the present utility model is further described.Following examples only for clearly the technical solution of the utility model being described, and can not limit protection domain of the present utility model with this.
Embodiment 1
As shown in accompanying drawing 1-2: a kind of tomographic system for high-purity ganglioside purifying, comprise sample introduction liquid storaging device, chromatographic column 1 and go out sample liquid storaging device, the first solid layer 10 and the second solid layer 12 of series coupled is successively provided with in described chromatographic column 1, described first solid layer 10 is filled by silica filler and is formed, and described second solid layer 12 is filled by gel filler and formed.Gel filler preferably but be not limited to SourceRPC30.
Described sample introduction liquid storaging device comprises sample liquid holding vessel 20, first eluent holding vessel 21 and the first constant flow pump 23, described sample liquid holding vessel 20 is connected by magnetic valve one end with the first constant flow pump 23 with the first eluent holding vessel 21, and the other end of described first constant flow pump 23 is connected with the porch of chromatographic column 1.
Embodiment 2
On the basis of embodiment 1, the utility model preferred embodiment is except following structure is different from embodiment 1, and other structures are all identical.
Shown in Fig. 3-4, be also provided with unhurried current layer 1 between described first solid layer 10 and the second solid layer 12, chromatographic column 1 post jamb being positioned at described unhurried current layer 11 is provided with the second eluent entrance 13; Described sample introduction liquid storaging device also comprises the second eluent storage tank 22 and the second constant flow pump 24, described second eluent storage tank 22 is connected by magnetic valve one end with the second constant flow pump 24, and the other end of described second constant flow pump 24 is connected with the second eluent entrance 13.
The upper end of described chromatographic column 1 is provided with upper cover plate 15, and lower end is provided with lower cover 16.Be provided with upper filter screen 17 between described upper cover plate 15 and chromatographic column 1, between described lower cover 16 and chromatographic column 1, be provided with lower filter screen 18.
Described go out sample liquid storage system comprise waste liquid tank 30 and qualified sample tank 31, described waste liquid tank 30 is connected respectively by the outlet of magnetic valve with chromatographic column 1 with qualified sample tank 31.The inside of described sample liquid holding vessel 20 is provided with elution device.
Described go out sample liquid storage system also comprise and intersect sample tank 32, described intersection sample tank 32 is connected by the outlet of magnetic valve with chromatographic column 1.
The surface of described chromatographic column 1 is provided with form 19.
The above is only preferred embodiment of the present utility model; should be understood that; for those skilled in the art; under the prerequisite not departing from the utility model know-why; can also make some improvements and modifications, these improvements and modifications also should be considered as protection domain of the present utility model.
Claims (9)
1. the tomographic system for high-purity ganglioside purifying, comprise sample introduction liquid storaging device, chromatographic column (1) and go out sample liquid storaging device, it is characterized in that, the first solid layer (10) and second solid layer (12) of series coupled is successively provided with in described chromatographic column (1), described first solid layer (10) is filled by silica filler and is formed, and described second solid layer (12) is filled by gel filler and formed;
Described sample introduction liquid storaging device comprises sample liquid holding vessel (20), the first eluent holding vessel (21) and the first constant flow pump (23), described sample liquid holding vessel (20) is connected by magnetic valve one end with the first constant flow pump (23) with the first eluent holding vessel (21), and the other end of described first constant flow pump (23) is connected with the porch of chromatographic column (1).
2. as claimed in claim 1 for the tomographic system of high-purity ganglioside purifying, it is characterized in that, also be provided with unhurried current layer (11) between described first solid layer (10) and the second solid layer (12), chromatographic column (1) post jamb being positioned at described unhurried current layer (11) is provided with the second eluent entrance (13); Described sample introduction liquid storaging device also comprises the second eluent storage tank (22) and the second constant flow pump (24), described second eluent storage tank (22) is connected by magnetic valve one end with the second constant flow pump (24), and the other end of described second constant flow pump (24) is connected with the second eluent entrance (13).
3., as claimed in claim 2 for the tomographic system of high-purity ganglioside purifying, it is characterized in that, between described unhurried current layer (11) and the second solid layer (12), be provided with filter plate (14).
4., as claimed in claim 1 for the tomographic system of high-purity ganglioside purifying, it is characterized in that, the upper end of described chromatographic column (1) is provided with upper cover plate (15), and lower end is provided with lower cover (16).
5. as claimed in claim 4 for the tomographic system of high-purity ganglioside purifying, it is characterized in that, be provided with upper filter screen (17) between described upper cover plate (15) and chromatographic column (1), between described lower cover (16) and chromatographic column (1), be provided with lower filter screen (18).
6. as claimed in claim 1 for the tomographic system of high-purity ganglioside purifying, it is characterized in that, described go out sample liquid storage system comprise waste liquid tank (30) and qualified sample tank (31), described waste liquid tank (30) is connected respectively by the outlet of magnetic valve with chromatographic column (1) with qualified sample tank (31).
7. as claimed in claim 6 for the tomographic system of high-purity ganglioside purifying, it is characterized in that, described go out sample liquid storage system also comprise intersect sample tank (32), described intersection sample tank (32) is connected by the outlet of magnetic valve with chromatographic column (1).
8., as claimed in claim 1 for the tomographic system of high-purity ganglioside purifying, it is characterized in that, the surface of described chromatographic column (1) is provided with form (19).
9., as claimed in claim 1 for the tomographic system of high-purity ganglioside purifying, it is characterized in that, the inside of described sample liquid holding vessel (20) is provided with elution device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520712333.7U CN204910866U (en) | 2015-09-15 | 2015-09-15 | Be used for purified chromatographic system of high -purity ganglioside |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520712333.7U CN204910866U (en) | 2015-09-15 | 2015-09-15 | Be used for purified chromatographic system of high -purity ganglioside |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204910866U true CN204910866U (en) | 2015-12-30 |
Family
ID=54960053
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520712333.7U Active CN204910866U (en) | 2015-09-15 | 2015-09-15 | Be used for purified chromatographic system of high -purity ganglioside |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204910866U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108854158A (en) * | 2017-05-16 | 2018-11-23 | 湖北生物医药产业技术研究院有限公司 | Resin column elutes liquor collecting device |
| CN116236819A (en) * | 2023-05-09 | 2023-06-09 | 成都佩德生物医药有限公司 | Method for purifying polypeptide toxins in batches and composite double-layer chromatographic column |
-
2015
- 2015-09-15 CN CN201520712333.7U patent/CN204910866U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108854158A (en) * | 2017-05-16 | 2018-11-23 | 湖北生物医药产业技术研究院有限公司 | Resin column elutes liquor collecting device |
| CN108854158B (en) * | 2017-05-16 | 2023-11-07 | 湖北生物医药产业技术研究院有限公司 | Resin column eluent collection device |
| CN116236819A (en) * | 2023-05-09 | 2023-06-09 | 成都佩德生物医药有限公司 | Method for purifying polypeptide toxins in batches and composite double-layer chromatographic column |
| CN116236819B (en) * | 2023-05-09 | 2023-08-04 | 成都佩德生物医药有限公司 | Method for purifying polypeptide toxins in batches and composite double-layer chromatographic column |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203108263U (en) | Double column circulating chromatographic system | |
| CN103394211B (en) | Apparatus for rapidly separating and purifying organic compounds and separation and purification method | |
| CN204910866U (en) | Be used for purified chromatographic system of high -purity ganglioside | |
| CN104383784B (en) | The system and method for separation and Extraction inert gas from environmental gas | |
| CN101940289B (en) | Method for separating discolored chili extract from chili pigment in crude products of chili extract | |
| CN103157295A (en) | Double-column recycling chromatographic system | |
| CN101386019A (en) | Cyclic regeneration method for repairing cyclodextrin eluent in polycyclic aromatic hydrocarbon contaminated soil | |
| CN204261380U (en) | A kind of adsorption separation device of purifying chlorogenic acid | |
| CN202942682U (en) | Solid-phase extraction column | |
| CN104974013B (en) | A kind of technique of utilization continuous chromatography technology separating butanol zymotic fluid | |
| CN103877747A (en) | Solid-phase extraction column | |
| CN205607942U (en) | A solid -phase extraction post for separating cr ( III ) and cr ( VI ) | |
| CN103446778B (en) | Ion exchange resin chromatography method and device without being promoted by external power | |
| CN112546718B (en) | Membrane separation system for gardenia extract | |
| CN206045474U (en) | The continuous desalination of automatization or decoloration device | |
| CN102336799A (en) | Simulated moving bed chromatography separation method for preparing ginsenoside Rb1 | |
| CN204543641U (en) | A kind of Simple solid phase extraction device | |
| CN207856383U (en) | A kind of column chromatography purifier apparatus | |
| CN103690587B (en) | The preparation method of triterpenoid saponin component | |
| TWM575357U (en) | Component separation and purification device | |
| CN202822867U (en) | Chromatographic column | |
| CN111944007A (en) | Method for purifying saponin | |
| CN205412434U (en) | Pressure pole filter equipment | |
| CN203342438U (en) | Plant extracting chromatographic purification device | |
| CN203494222U (en) | Ion exchange resin chromatography device without propelling of external power |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |