CN203108263U

CN203108263U - Double column circulating chromatographic system

Info

Publication number: CN203108263U
Application number: CN 201320124932
Authority: CN
Inventors: 周胜; 王勇; 鲁海波; 刘红萍
Original assignee: LISURE SCIENCE (SUZHOU) CO Ltd
Current assignee: LISURE SCIENCE (SUZHOU) CO Ltd
Priority date: 2013-03-19
Filing date: 2013-03-19
Publication date: 2013-08-07
Anticipated expiration: 2023-03-19

Abstract

The utility model relates to a double column circulating chromatographic system, which comprises a first elution pump, a second elution pump and a sampling pump, wherein the first elution pump and the second elution pump are both connected with a first six-position three-way valve; the six-position three-way valve is connected with a first one-way six-position valve and a second one-way six-position valve; the first one-way six-position valve and the second one-way six-position valve are respectively connected with a first chromatographic column and a second chromatographic column; and the first chromatographic column and the second chromatographic column are respectively connected with a first ultraviolet detector and a second ultraviolet detector. With the adoption of the double column circulating chromatographic system, the sample separation rate is improved, the adsorbing capacity of an adsorption column is improved, and the sample separation rate and the adsorbing capacity by the double column circulating chromatographic system are improved by 30-50% compared with the sample separation rate and the adsorbing capacity by the traditional chromatographic system; and the separation of the samples which are difficult to separate can be realized in a way that components which are not separated can be adsorbed and desorbed repeatedly, so that matters which are relatively difficult to separate can be easily separated, and the yield and the purity achieve higher levels.

Description

Twin columns cyclical chromatography system

Technical field

The utility model relates to the chromatographic system of industries such as chemical industry, pharmacy, natural products purifying, especially relates to a kind of twin columns cyclical chromatography system.

Background technology

Traditional adsorption chromatography system and preparative chromatography system all are that single-column uses separately, can not realize that like this circulation absorption of chromatogram is continuous with preparation continuously, and absorption and preparative chromatography efficient are low, and elute soln consumption is big in manufacturing process.

Summary of the invention

In order to overcome above-mentioned defective, the utility model provides a kind of twin columns cyclical chromatography system, has realized circulation continuous adsorption and preparative chromatography, has improved operating efficiency greatly and the consumption of elute soln is reduced.

The technical scheme that its technical problem that solves the utility model adopts is: a kind of twin columns cyclical chromatography system, comprise the first wash-out pump, the second wash-out pump and last sample pump, the first wash-out pump all is connected the one or six position three-way valve with the second wash-out pump, the one or six position three-way valve connects the one one logical six valves and the 21 logical six valves, the one one logical six valves are connected first chromatographic column and second chromatographic column respectively with the 21 logical six valves, first chromatographic column is connected first UV-detector and second UV-detector again respectively with second chromatographic column, first UV-detector is connected the 31 logical six valves and the 41 logical six valves respectively with second UV-detector, last sample pump is connected to the two or six position three-way valve, the 31 logical six valves and the 41 logical six valves all are connected weak eluent and strong eluent and collect mouthful, the 31 logical six valves and the 41 logical six valves are connected to pure product by pipeline and collect mouth, are provided with the pH detector in the pipeline.

The beneficial effects of the utility model are: adopt twin columns cyclical chromatography system, improved the sample separation rate, and improve separative efficiency; Can improve simultaneously the adsorbance of adsorption column, improve 30-50% than traditional chromatographic system.It is similar that the twin columns circulatory system has also solved some structural properties, and be difficult to the separation of sample separation, can make the component of not separating carry out the multiple adsorb desorb by repeatedly circulating, thereby isolate the difficult material that separates easily.Make yield and purity all reach higher level.

Description of drawings

Fig. 1 is the utility model preparative liquid chromatography preparatory stage schematic diagram;

Fig. 2 is the utility model preparative liquid chromatography first step;

Fig. 3 is second step of the utility model preparative liquid chromatography;

Fig. 4 is the 3rd step of the utility model preparative liquid chromatography;

Fig. 5 is the 4th step of the utility model preparative liquid chromatography;

Fig. 6 is the 5th step of the utility model preparative liquid chromatography;

Fig. 7 is the 6th step of the utility model preparative liquid chromatography;

Fig. 8 is the 7th step of the utility model preparative liquid chromatography;

Fig. 9 is the 8th step of the utility model preparative liquid chromatography;

Figure 10 is the 9th step of the utility model preparative liquid chromatography;

Figure 11 is the tenth step of the utility model preparative liquid chromatography;

Figure 12 is the 11 step of the utility model preparative liquid chromatography;

Figure 13 is the 12 step of the utility model preparative liquid chromatography;

Figure 14 is the 13 step of the utility model preparative liquid chromatography;

Figure 15 is the 14 step of the utility model preparative liquid chromatography;

Figure 16 is the utility model preparative liquid chromatography system operational flow diagram.

Among the figure: 1, the first wash-out pump; 2, the second wash-out pump; 3, the one or six position three-way valve; 4, the one one logical six valves; 5, the two or six position three-way valve; 6, the 21 logical six valves; 7, first chromatographic column; 8, second chromatographic column; 9, first UV-detector; 10, second UV-detector; 11, go up the sample pump; 12, the 31 logical six valves; 13, the 41 logical six valves; 14, PH detector; 15, weak eluent is collected mouth; 16, pure product are collected mouth; 17, strong eluent is collected mouth; 18, cleaning fluid.

The specific embodiment

Below in conjunction with the drawings and the specific embodiments the utility model is described in detail: extremely shown in Figure 15 as Fig. 1, heavy line is represented path among the figure, fine line is represented not path, a kind of twin columns cyclical chromatography system, comprise the first wash-out pump 1, the second wash-out pump 2 and last sample pump 11, the described first wash-out pump 1 all is connected the one or six position three-way valve 3 with the second wash-out pump 2, the one or six position three-way valve 3 connects the one one logical six valves 4 and the 21 logical six valves 6, the one one logical six valves 4 are connected first chromatographic column 7 and second chromatographic column 8 respectively with the 21 logical six valves 6, first chromatographic column 7 is connected first UV-detector 9 and second UV-detector 10 again respectively with second chromatographic column 8, first UV-detector 9 is connected the 31 logical six valves 12 and the 41 logical six valves 13 respectively with second UV-detector 10, last sample pump 11 is connected to the two or six position three-way valve 5, the 31 logical six valves 12 all are connected weak eluent collection mouth 15 with the 41 logical six valves 13 and strong eluent is collected mouth 17, the 31 logical six valves 12 and the 41 logical six valves 13 are connected to pure product by pipeline and collect mouth 16, are provided with pH detector 14 in the pipeline.

Preparative liquid chromatography system operational flow diagram at first is the preparatory stage as shown in figure 16, cleans by 18 pairs of systems of cleaning fluid behind the connecting path again; Second step was respectively first chromatographic column 7 and second chromatographic column 8 to be moistened post by the first wash-out pump 1 and the second wash-out pump 2; The 3rd step was to utilize last sample pump that first chromatographic column is gone up sample; The 4th step was first chromatographic column to be carried out wash-out separate, because the solution in first chromatographic column is three layers, namely bottom is preceding impurity layer, after be pure product layer and rear impurity layer, so the 5th step was to separate impure product in first chromatographic column, namely preceding impurity was forwarded in second chromatographic column; The 6th step was collected sample to the second chromatographic column on the sample pump on this moment exactly to the pure product in first chromatographic column afterwards; The 7th step was exactly that the interior rear impurity of first chromatographic column forwards second chromatographic column to; The 8th step was that first chromatographic column is cleaned, and simultaneously second chromatographic column was begun wash-out; The 9th step was that the second wash-out pump moistens post to first chromatographic column, and the second chromatographic column wash-out separates simultaneously; The tenth step was that the preceding impurity in second chromatographic column goes to first chromatographic column; The 11 step was that second chromatographic column is carried out wash-out and collected pure product; The 12 step was that the rear impurity in second chromatographic column goes in first chromatographic column, was that second chromatographic column is cleaned at last, and first chromatographic column begins wash-out simultaneously, begins the circulation in the 5th step to the 13 step thus.

Twin columns cyclical chromatography system configuration two kinds of method modes, be respectively twin columns circulation adsorption chromatography and twin columns cycles prepare chromatogram, respectively as follows:

Twin columns circulation adsorption chromatography method: can use separately with the single chromatographic column of traditional adsorption chromatography system, realize singly pulling on sample, absorption, desorb, the whole process of regeneration; Built-in workstation software can be by simply dragging favored area, automatically convert traditional single-column batch technology to two chromatographic column circulation absorption continuous processings, two chromatographic column circulation absorption continuous processings are than single-column batch technology, improved the adsorption efficiency of adsorption chromatography medium greatly, applied sample amount can improve 30%-50%; And elute soln consumption reduces in the technology; Can also reclaim various dissimilar elute solns automatically, the convenient processing.

Twin columns cycles prepare chromatographic process: can use separately with traditional preparative chromatography system single-column, realize singly pulling on sample, the whole process of wash-out; Built-in workstation software can convert traditional single-column batch technology to twin columns cycles prepare continuous processing, than single-column batch technology automatically by simply dragging favored area, realized the continuous sample introduction preparation, for the less impurity of content, can realize repeatedly enrichment, the separative efficiency height; Elute soln consumption reduces in the technology; Can reclaim various dissimilar elute solns automatically, the convenient processing.

This system can be used for various low pressure, medium and high pressure chromatographic applications, is fit to various adsorbing mediums such as ion-exchange, and hydrophobic effect is anti-phase, molecular sieve, and affine filler, system is applicable to laboratory, pilot scale and production level scale.

It is emphasized that: above only is preferred embodiment of the present utility model, be not that the utility model is done any pro forma restriction, every foundation technical spirit of the present utility model all still belongs in the scope of technical solutions of the utility model any simple modification, equivalent variations and modification that above embodiment does.

Claims

1. twin columns cyclical chromatography system, it is characterized in that: comprise the first wash-out pump, the second wash-out pump and last sample pump, the described first wash-out pump all is connected the one or six position three-way valve with the second wash-out pump, described the one or six position three-way valve connects the one one logical six valves and the 21 logical six valves, the described the 1 logical six valves are connected first chromatographic column and second chromatographic column respectively with the 21 logical six valves, described first chromatographic column is connected first UV-detector and second UV-detector again respectively with second chromatographic column, described first UV-detector is connected the 31 logical six valves and the 41 logical six valves respectively with second UV-detector, the described sample pump of going up is connected to the two or six position three-way valve, the described the 31 logical six valves and the 41 logical six valves all are connected weak eluent and strong eluent and collect mouthful, the described the 31 logical six valves and the 41 logical six valves are connected to pure product by pipeline and collect mouth, are provided with the pH detector in the described pipeline.