CN210583782U - Column chromatography integrated system for continuous sample loading - Google Patents
Column chromatography integrated system for continuous sample loading Download PDFInfo
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- CN210583782U CN210583782U CN201921338873.8U CN201921338873U CN210583782U CN 210583782 U CN210583782 U CN 210583782U CN 201921338873 U CN201921338873 U CN 201921338873U CN 210583782 U CN210583782 U CN 210583782U
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Abstract
The utility model discloses a go up column chromatography integrated system of appearance in succession, column chromatography integrated system is including stock solution unit, the pump unit, rotatable interface and the column chromatography unit that the series connection set up. The liquid storage unit is connected with the pump unit through a liquid storage tank outlet three-way valve, and the pump unit is connected with the three-way valve of the chromatographic column unit through a rotatable interface. The column chromatography unit comprises two or more stages of chromatography columns connected in series and accessory structures thereof, and feed liquid outlets are arranged at the inlet and outlet of each chromatography column. The system is suitable for being equipped with ion exchange media, affinity chromatography media, hydrophobic chromatography media and the like, is used for continuous engineering separation and purification of complex mixtures such as sugars, proteins and nucleic acids, is changed through a channel of a rotatable column chromatography unit matched with a three-way valve, is convenient and fast to realize cylinder regeneration and prolong the service life of the separation media to the maximum extent, and is simple in process, convenient and fast to operate, efficient and practical.
Description
Technical Field
The utility model belongs to the field of medical engineering equipment, concretely relates to column chromatography integrated system who goes up appearance in succession.
Background
The column chromatography is a technical means for separating the mixture according to the difference of the partition coefficients of each component in the mixture in a stationary phase and a mobile phase. The chromatography is classified according to its mechanism into adsorption chromatography, partition chromatography, ion exchange chromatography, gel filtration chromatography, affinity chromatography, etc.
The column chromatography technology is widely applied to the fields of biology, medicine, food and the like, and can be used for separating and purifying carbohydrate, protein and nucleic acid mixtures with complex components. However, the application of column chromatography technology in industrialization is still restricted by the problems of low utilization efficiency, low purification efficiency, low product yield and the like of the column chromatography in the application process of column chromatography. In the field of biological medicine, the advanced column chromatography technology and column chromatography equipment design can help pharmaceutical manufacturers to solve the huge capacity challenge faced by the pharmaceutical manufacturers.
According to the separation mechanism of the chromatographic column medium, such as an ion exchange medium, an affinity chromatographic medium, a hydrophobic chromatographic medium and the like, only the target component or the impurity component can be eluted by means of controlling the mobile phase, and the corresponding impurity component or the target component is still adsorbed on the stationary phase. Thus, continuous sample loading is possible on the premise that the sample loading amount of the separation medium allows, and the separation efficiency can be greatly improved. Based on this, the utility model provides a can go up appearance column chromatography integrated system design scheme in succession, this scheme not only can realize going up appearance in succession, and the activation that also is convenient for chromatography medium is handled, has greatly increased the life of chromatography efficiency and chromatography medium, reduces product manufacturing cost.
Disclosure of Invention
An object of the utility model is to provide a column chromatography integrated system who goes up appearance in succession, the column chromatography system is applicable to the product separation and purification that can go up the separation of appearance in succession, and accessible column chromatography unit upset cooperation valve passageway transform from top to bottom realizes just dashing and recoil of chromatography post.
In order to achieve the above object, the utility model provides a following technical scheme:
the utility model provides a column chromatography integrated system who goes up appearance in succession, column chromatography integrated system is including stock solution unit, the pump unit, the mouth that can rotate and the column chromatography unit that establish ties and set up, the stock solution unit passes through stock solution unit export three-way valve and is connected with the pump unit, the pump unit is connected with the column chromatography unit through the mouth that can rotate.
Further, the liquid storage unit comprises a liquid storage tank, a liquid storage unit outlet three-way valve and a liquid storage tank liquid outlet.
Furthermore, one end of the three-way valve at the outlet of the liquid storage unit is connected with the liquid storage tank, the other end of the three-way valve is connected with the liquid outlet of the liquid storage tank, and the other end of the three-way valve is connected with the pump unit.
Furthermore, the pump unit is an adjustable-speed constant flow pump or a pump system consisting of an adjustable-speed pulse pump and a liquid pulse damper which are arranged in series.
Further, the column chromatography unit comprises a chromatography column, a control valve and a feed liquid outlet.
Furthermore, the chromatographic columns are connected in series in 2 stages or more than 2 stages.
Further, the control valves comprise 1 three-way valve and 4 two-way valves;
furthermore, rotatable joint is connected to three-way valve one end, and one end is passed through 1 two-way valve and is connected first order chromatography column front end, and one end is passed through 1 two-way valve and is connected last stage chromatography column rear end.
Furthermore, the front end of the first-stage chromatographic column and the rear end of the last-stage chromatographic column are respectively connected with a feed liquid outlet through 1 two-way valve.
Further, the integrated system for column chromatography is equipped with ion exchange media, affinity chromatography media or hydrophobic chromatography media.
Further, the column chromatography unit is wholly turned over up and down through the rotatable joint.
Compared with the prior art, the utility model discloses an advantage is with positive effect:
(1) the utility model discloses divide into 2 grades or the chromatography column more than 2 grades with the chromatography column unit and connect with the mode of establishing ties, under the complicated mixture separation effect prerequisite such as having guaranteed sugars, albumen, greatly increased the use number of times of chromatography column medium. Particularly, for the separation and purification of substances which are adsorbed or difficult to elute, when the first-stage chromatographic column is adsorbed to the maximum sample carrying capacity, the second-stage chromatographic column can be changed to the first-stage chromatographic column, the third-stage chromatographic column can be changed to the second-stage chromatographic column, and the like. The mode avoids the complex process that only a grade 1 large column is assembled under the same volume of separation medium and needs to be fastened and reloaded; also ensures that the available separation medium is kept at a certain quantifiable level, and ensures the separation and purification effect.
(2) A speed-adjustable constant flow pump can be installed in the pump unit, and a speed-adjustable pulse pump can also be installed and connected in series to be provided with a liquid pulse damper, so that the liquid to be separated can enter the column chromatography unit at a constant speed, and the separation effect is ensured.
(3) Through the design that can overturn from top to bottom of column chromatography unit, the passageway of cooperation chromatography column entry three-way valve and column chromatography unit two-way valve is changed, has guaranteed the column chromatography unit just towards the feasibility with the recoil, and realization sample that can convenient and fast is purified in succession, separation medium regeneration, simple process, simple operation, in a flexible way, high-efficient, practical.
Drawings
Fig. 1 is a schematic diagram of a column chromatography integrated system for continuous sample loading according to the utility model.
Fig. 2 is a schematic diagram of the column chromatography integrated system for continuous sample loading after the column chromatography unit is turned over.
Wherein: A. the device comprises a liquid storage unit, a pump unit, a column chromatography unit, a rotatable interface and a control unit, wherein the liquid storage unit comprises a liquid storage unit, a pump unit, a column chromatography unit and a rotatable interface;
1. a liquid storage tank, 2, a speed-adjustable pulse pump, 3 and a liquid pulse damper;
c1, a first-stage chromatographic column, c2 and a second-stage chromatographic column;
t1, a three-way valve for an outlet of the liquid storage unit, t2, a three-way valve for an inlet of the chromatography unit;
v 1-v 4 are column chromatography unit column front and rear two-way valves respectively;
e1, a liquid outlet of the liquid storage tank, e2, a liquid outlet at the back of the chromatographic column, e3 and a liquid outlet at the front of the chromatographic column.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments obtained by those skilled in the art without any creative work based on the present embodiment belong to the protection scope of the present invention.
Example 1
Referring to fig. 1, the utility model discloses a column chromatography integrated system that sample was gone up in succession, column chromatography integrated system is including stock solution unit A, pump unit B, column chromatography unit C and the rotatable interface D that establish ties and set up.
The liquid storage unit A comprises a liquid storage tank 1, a liquid storage unit outlet three-way valve t1 and a liquid storage tank liquid outlet e 1. Liquid storage unit outlet three-way valve t1 is located between liquid storage tank 1 and liquid storage tank outlet e 1. One end of a three-way valve t1 at the outlet of the liquid storage unit is connected with the liquid storage tank 1, one end of the three-way valve t1 is connected with a liquid outlet e1 of the liquid storage tank, and the other end of the three-way valve t is connected with the pump unit B. By controlling the three-way valve t1 at the outlet of the liquid storage unit, the liquid in the liquid storage tank 1 can be selected to flow through the pump unit B or the liquid outlet e1 of the liquid storage tank.
The pump unit B can be a speed-adjustable constant-flow pressure pump, the upper end of the pump unit B is connected with a liquid storage unit outlet three-way valve t1 in the liquid storage unit A, and the lower end of the pump unit B is connected with a chromatography unit inlet three-way valve t2 of the column chromatography unit C. The pump unit B may also be a pump system consisting of a variable speed pulse pump 2 and a liquid pulse damper 3. The adjustable-speed pulse pump 2 and the liquid pulse damper 3 are connected in series, the upper end of the adjustable-speed pulse pump 2 is connected with a liquid storage unit outlet three-way valve t1 in the liquid storage unit A, and the lower end of the liquid pulse damper 3 is connected with a chromatography unit inlet three-way valve t2 of the column chromatography unit C. By adopting the scheme, the feed liquid can enter the chromatographic column at a constant speed, and the separation effect is ensured. The integrated column chromatography system can be equipped with ion exchange media, affinity chromatography media or hydrophobic chromatography media, etc.
The column chromatography unit C comprises a chromatography column, a control valve and a feed liquid outlet. The chromatographic columns are connected in a series connection mode at 2 levels or more than 2 levels, and the adoption of the mode of connecting a plurality of chromatographic columns in series not only avoids the complex process that only 1-level large columns are assembled under the same amount of separation media, but also needs to be re-assembled by glue; also ensures that the available separation medium is kept at a certain quantifiable level, and has high practicability for separating and purifying complex mixtures such as saccharides, proteins, nucleic acids and the like. The inlet three-way valve t2 of the chromatographic unit is connected with the front end of the first-stage chromatographic column c1 through a two-way valve v1 and is connected with the rear end of the last-stage chromatographic column through a two-way valve v 2. A two-way valve v4 is connected in parallel between the two-way valve v1 and the front end of the first-stage chromatographic column c1, and a front liquid outlet e3 of the chromatographic column is connected with the two-way valve v 4; and a two-way valve v3 is connected in parallel between the two-way valve v2 and the rear end of the last-stage chromatographic column, and a rear liquid outlet e2 of the chromatographic column is connected with the two-way valve v 3.
A rotatable port D is located between the pump unit B and the chromatography unit inlet three-way valve t 2. The function of the column chromatography unit C is to allow the column chromatography unit C to be turned upside down integrally. The effect after the up-down turning is as follows: the rear end of the last-stage chromatographic column is located at the upper end, and the front end of the first-stage chromatographic column is located at the lower end, as shown in fig. 2. The design is suitable for the regeneration of the chromatographic column after the separation and purification of the dead adsorbed sample. When the first-stage chromatographic column is subjected to dead adsorption, the column chromatography unit C can be integrally turned over, and the back flushing of the chromatographic column is carried out by adjusting a valve passage, so that the pollution of the subsequent chromatographic column can be reduced to the maximum extent, and the service life of the separation medium is prolonged.
Example 2
If the substance to be separated does not contain the dead adsorption component, the procedures of separation and purification and chromatographic column regeneration are as follows:
(1) preparation of
Referring to fig. 1, the liquid storage tank 1 is cleaned with a solution of a substance to be purified, and then the cleaning liquid is discharged through a flow path of the liquid storage tank 1 → the outlet three-way valve t1 of the liquid storage unit → the liquid outlet e1 of the liquid storage tank. Then, an appropriate amount of the above-mentioned solution is added, and column equilibrium is performed according to a liquid storage tank 1 → a liquid storage unit outlet three-way valve t1 → a pump unit B → a chromatography unit inlet three-way valve t2 → a two-way valve v1 → a first-stage chromatography column c1 → a second-stage chromatography column c2 → a two-way valve v3 → a post-column liquid outlet e2 passage (wherein the two-way valves v2 and v4 are in a closed state).
(2) Continuous separation
Referring to fig. 1, after the chromatographic column is balanced, the feed liquid to be purified is stored in a liquid storage tank 1, and continuous sample loading and separation are performed according to the flow path in step (1). At this time, the retained fraction is accumulated and retained on the first-stage chromatography column c1, and the non-retained fraction is discharged through the column effluent port e2 and collected. After the separation of the purified material liquid is finished, a proper amount of dissolving liquid of the material to be purified is added into the liquid storage tank 1, and the non-reserved components in the system are continuously flushed out according to the flow path.
(3) Chromatographic column regeneration
Referring to fig. 1, after the sample loading is finished, according to the nature of the retained component, preparing a suitable eluent to store in the liquid storage tank 1, eluting the component retained on the chromatographic column according to the flow path described in step (1), discharging through the chromatographic column effluent port e2, and collecting.
Example 3
If the substance to be separated contains dead adsorption or extremely difficult elution components, the regeneration process of the chromatographic column is as follows:
(1) preparation of
Referring to fig. 1, the liquid storage tank 1 is cleaned with a solution of a substance to be purified, and then discharged through a flow path of the liquid storage tank 1 → the outlet three-way valve t1 of the liquid storage unit → the liquid outlet e1 of the liquid storage tank. Then, an appropriate amount of the above-mentioned solution is added, and column equilibrium is performed by a liquid reservoir tank 1 → a liquid reservoir unit outlet three-way valve t1 → a pump unit B → a chromatography unit inlet three-way valve t2 → a two-way valve v1 → a first-stage chromatography column c1 → a second-stage chromatography column c2 → a two-way valve v3 → a post-column liquid outlet e2 passage (wherein the two-way valves v2 and v4 are in a closed state).
(2) Continuous separation
Referring to fig. 1, after the chromatographic column is balanced, the liquid to be purified is contained in a liquid storage tank 1, and continuous sample loading and separation are performed according to the balanced flow path in the step (1). At this time, the retained component is accumulated and retained on the first-stage chromatographic column c1, and the non-retained component is discharged from the liquid outlet e2 after passing through the chromatographic column and collected. After the separation of the purified material liquid is finished, a proper amount of solution of the material to be purified is added into the liquid storage tank 1, and the non-reserved component in the system is continuously flushed out according to the flow path.
(3) Chromatographic column regeneration
Referring to fig. 2, after the sample loading is finished, an appropriate eluent is prepared and contained in the liquid storage tank 1 according to the properties of the reserved components. Rotating the column chromatography unit C, and exchanging the column chromatography unit C up and down. The components retained on the column are eluted by a path of the liquid storage tank 1 → the liquid storage unit outlet three-way valve t1 → the pump unit B → the chromatography unit inlet three-way valve t2 → the two-way valve v2 → the second-stage chromatography column c2 → the first-stage chromatography column c1 → the two-way valve v4 → the column front liquid outlet e3 (wherein the two-way valves v1 and v3 are in a closed state), discharged through the column front liquid outlet e3 and collected.
The mode can ensure that the chromatographic column after the second stage is not polluted by dead adsorption or difficult-to-elute components of the adsorbent as far as possible, and the service life of the chromatographic medium is fully prolonged. When the first-stage chromatographic column is completely dead-absorbed, the second-stage chromatographic column can be used for supplementing the position to the first-stage chromatographic column, and then the positions are sequentially supplemented, and the last-stage chromatographic column is replaced by a new column.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (10)
1. The integrated system for column chromatography for continuous sample loading is characterized in that: column chromatography integrated system is including stock solution unit, the pump unit, the rotatable interface and the column chromatography unit that establish ties and set up, the stock solution unit passes through stock solution unit export three-way valve and is connected with the pump unit, the pump unit is connected with the column chromatography unit through the rotatable interface.
2. The integrated system for column chromatography in continuous loading according to claim 1, wherein: the liquid storage unit comprises a liquid storage tank, a liquid storage unit outlet three-way valve and a liquid storage tank liquid outlet.
3. The integrated system for column chromatography in continuous loading according to claim 2, wherein: one end of the three-way valve at the outlet of the liquid storage unit is connected with the liquid storage tank, one end of the three-way valve is connected with the liquid outlet of the liquid storage tank, and the other end of the three-way valve is connected with the pump unit.
4. The integrated system for column chromatography of continuous sample loading according to claim 1, wherein the pump unit is a constant flow pump with adjustable speed or a pump system consisting of an adjustable speed pulse pump and a liquid pulse damper arranged in series.
5. The integrated system for column chromatography in continuous loading according to claim 1, wherein the column chromatography unit comprises a chromatography column, a control valve and a feed liquid outlet.
6. The integrated system for column chromatography capable of realizing continuous sample loading according to claim 5, wherein the chromatographic columns are connected in series in 2 stages or more than 2 stages.
7. The integrated system for column chromatography in continuous loading according to claim 6, wherein the control valve comprises 1 three-way valve and 4 two-way valves.
8. The integrated system for column chromatography in continuous loading of claim 7, wherein the three-way valve has one end connected to the rotatable joint, one end connected to the front end of the first-stage column through 1 two-way valve, and one end connected to the rear end of the last-stage column through 1 two-way valve.
9. The integrated system for column chromatography of continuous sample loading according to claim 8, wherein the front end of the first-stage chromatographic column and the rear end of the last-stage chromatographic column are respectively connected with a feed liquid outlet through 1 two-way valve.
10. The integrated system for column chromatography in continuous loading according to claim 1, wherein: the column chromatography integrated system is provided with an ion exchange medium, an affinity chromatography medium or a hydrophobic chromatography medium.
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| CN201921338873.8U CN210583782U (en) | 2019-08-19 | 2019-08-19 | Column chromatography integrated system for continuous sample loading |
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| CN201921338873.8U CN210583782U (en) | 2019-08-19 | 2019-08-19 | Column chromatography integrated system for continuous sample loading |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116077990A (en) * | 2023-04-12 | 2023-05-09 | 江苏中慧元通生物科技股份有限公司 | Sample injection device for chromatography system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116077990A (en) * | 2023-04-12 | 2023-05-09 | 江苏中慧元通生物科技股份有限公司 | Sample injection device for chromatography system |
| CN116077990B (en) * | 2023-04-12 | 2023-06-16 | 江苏中慧元通生物科技股份有限公司 | Sample injection device for chromatography system |
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