CN215250532U

CN215250532U - Protein chromatography device

Info

Publication number: CN215250532U
Application number: CN202120783489.XU
Authority: CN
Inventors: 李大为
Original assignee: Suzhou Yingsaisi Intelligent Technology Co ltd
Current assignee: Suzhou Yingsaisi Intelligent Technology Co ltd
Priority date: 2021-04-16
Filing date: 2021-04-16
Publication date: 2021-12-21
Anticipated expiration: 2031-04-16

Abstract

The utility model relates to a protein chromatography device, include: the inlet valve comprises a first selection switch, a first liquid outlet and a plurality of first liquid inlets, and the first selection switch rotates to realize the communication between the first liquid inlets and the first liquid outlets; the infusion pump is connected with the first liquid outlet to convey the sample or the buffer solution; the sample injection unit is connected with the infusion pump and comprises a sample injection valve and a sample ring for pre-loading a sample, and the sample injection valve is connected with the sample ring; the chromatography unit is connected with the sample introduction unit to perform chromatography operation on a sample in the sample introduction unit and comprises a single-column position valve and a first chromatography column, and the single-column position valve controls whether liquid in the sample introduction unit flows through the first chromatography column or not; an outlet valve connected with the output end of the chromatography unit; a collector connected to the outlet valve to collect the target protein component. The automatic control system is high in automation degree, high in working efficiency and low in use cost.

Description

Protein chromatography device

Technical Field

The utility model relates to the technical field of protein chromatography, in particular to a protein chromatography device.

Background

Protein chromatographic purification systems are commonly used to separate a protein of interest from a solution containing the protein of interest and one or more impurities. Typically, protein chromatographic purification systems require separation techniques that utilize different principles, require the use of different chromatographic columns, and require the association of chromatographic columns through which the protein solution flows in a particular sequence. For example, a protein chromatographic purification system may use some or all of the following 5 column types in sequence: affinity column, desalting column, ion exchange column and gel filtration column.

In the traditional method, target components eluted from each chromatographic column are collected, recombined and injected into a chromatographic system again for separation again by using another chromatographic column, and for complex samples, the complex samples need to be separated for many times, and an operator needs to frequently perform repetitive work, thereby wasting time and labor.

SUMMERY OF THE UTILITY MODEL

Therefore, the technical problem to be solved by the utility model lies in overcoming the technical defects of complex operation, time and labor waste of protein chromatography purification in the prior art.

In order to solve the technical problem, the utility model provides a protein chromatography device, include:

the inlet valve comprises a first selection switch, a first liquid outlet and a plurality of first liquid inlets, and the first selection switch rotates to realize the communication between the first liquid inlets and the first liquid outlets;

the infusion pump is connected with the first liquid outlet to convey the sample or the buffer solution;

the sample injection unit is connected with the infusion pump and comprises a sample injection valve and a sample ring for pre-loading a sample, and the sample injection valve is connected with the sample ring;

the chromatography unit is connected with the sample introduction unit to perform chromatography operation on a sample in the sample introduction unit and comprises a single-column position valve and a first chromatography column, and the single-column position valve controls whether liquid in the sample introduction unit flows through the first chromatography column or not;

an outlet valve connected with the output end of the chromatography unit;

a collector connected to the outlet valve to collect the target protein component.

Preferably, the chromatography unit has a plurality of sets, and the plurality of sets of chromatography units are arranged in series.

Preferably, the first columns of each set of said chromatography units are different.

Preferably, the chromatography unit further comprises a multi-column valve and a plurality of second chromatography columns, wherein the multi-column valve is connected with the plurality of second chromatography columns to control the sample liquid to flow through the preset second chromatography columns.

Preferably, the chromatography device further comprises a detection unit, wherein the detection unit is arranged between the chromatography unit and the outlet valve.

Preferably, the detection unit is an ultraviolet detector, a conductivity detector or a PH detector.

Preferably, the device also comprises a sample liquid bottle, a balance liquid bottle and an elution liquid bottle, wherein the sample liquid bottle, the balance liquid bottle and the elution liquid bottle are arranged in one-to-one correspondence with the first liquid inlet.

Preferably, the first chromatography column is an affinity column, a desalting column, an ion exchange column or a gel filtration column.

Preferably, the infusion pump has one or more.

Preferably, the number of the first liquid inlet of the inlet valve is 4-6.

Compared with the prior art, the technical scheme of the utility model have following advantage:

1. the utility model discloses a set up inlet valve, transfer pump, advance kind unit, chromatography unit, outlet valve and collector, through mutually supporting of each part, can be so that the separation and purification work of protein can once only the full automatization accomplish, provide work efficiency, reduce use cost.

2. The utility model discloses an inlet valve and transfer pump cooperation can be selectively to various liquid of system internal input, and the sample ring in the appearance unit of advancing can be used to the pre-installation sample, the sample of pre-installation gets into the chromatography unit under the effect of transfer pump, and in the chromatography unit, through the switching of single column position valve, can make the sample flow through or not flow through first chromatography post, and the chromatography unit can set up a plurality ofly, so, can realize the multiple chromatography operation of sample, and at last, during liquid flowed into the collector from the outlet valve, the collector can collect the target protein component.

Drawings

In order to make the content of the present invention more clearly understood, the present invention will be described in further detail with reference to the following embodiments of the present invention, in conjunction with the accompanying drawings.

FIG. 1 is a schematic diagram of the structure of the protein chromatography device of the present invention;

fig. 2 is a schematic view of the inlet valve.

The specification reference numbers indicate: 10. an inlet valve; 11. a first liquid inlet; 12. a first liquid outlet; 13. a first selection switch; 20. an infusion pump; 30. a sample injection valve; 31. a sample loop; 40. a chromatography unit; 41. a single-column-position valve; 42. a first chromatography column; 50. an outlet valve; 60. a collector; 70. an ultraviolet detector; 71. a conductivity detector; 72. a ph detector; 80. a sample liquid bottle; 81. a balancing liquid bottle; 82. and (4) an eluent bottle.

Detailed Description

The present invention is further described with reference to the following drawings and specific embodiments so that those skilled in the art can better understand the present invention and can implement the present invention, but the embodiments are not to be construed as limiting the present invention.

Referring to fig. 1, the present invention discloses a protein chromatography apparatus, which comprises an inlet valve 10, an infusion pump 20, a sample injection unit, a chromatography unit 40, an outlet valve 50 and a collector 60.

The inlet valve 10 includes a first selection switch 13, a first liquid outlet 12 and a plurality of first liquid inlets 11, and the first selection switch 13 rotates to realize the communication between the first liquid inlets 11 and the first liquid outlets 12. The utility model discloses in, can be at first inlet 11 through establishing sample liquid, first balanced liquid, second balanced liquid, first eluant and second eluant, every first inlet 11 can be through establishing a liquid, rotate through first selection switch 13, can realize certain first inlet 11 and first liquid outlet 12 intercommunication selectively. As shown in fig. 2, which is a schematic structural diagram of the inlet valve 10, by rotating the first selection switch 13, a certain first liquid inlet 11 can be communicated with a certain first liquid outlet 12.

The infusion pump 20 is connected to the first liquid outlet 12 for delivering the sample or the buffer solution, and the liquid at the inlet valve 10 can be pumped to the subsequent sample injection unit and the chromatography unit 40 by the action of the infusion pump 20.

The sample injection unit is connected with the infusion pump 20, the sample injection unit comprises a sample injection valve 30 and a sample ring 31 for pre-loading a sample, and the sample injection valve 30 is connected with the sample ring 31. The sample loop 31 can be used to pre-load the sample, which is brought into the system by the infusion pump 20 by switching of the injection valve 30.

The chromatography unit 40 is connected with the sample introduction unit to perform chromatography operation on the sample in the sample introduction unit, the chromatography unit 40 comprises a single-column-position valve 41 and a first chromatography column 42, and the single-column-position valve 41 controls whether the liquid in the sample introduction unit flows through the first chromatography column 42.

An outlet valve 50 is connected to the output of the chromatography unit 40. The outlet valve 50 is provided with a second selector switch, which by turning can let the liquid flow out of the different outlets.

The collector 60 is connected to the outlet valve 50 to collect the target protein component.

The utility model discloses a theory of operation is: through the cooperation of the inlet valve 10 and the infusion pump 20, various liquids can be selectively infused into the system, the sample loop 31 in the sample injection unit can be used for pre-loading samples, the pre-loaded samples enter the chromatography unit 40 under the action of the infusion pump 20, in the chromatography unit 40, the samples can flow through or not flow through the first chromatography column 42 through the switching of the single-column position valve 41, and a plurality of chromatography units 40 can be arranged, so that various chromatography operations of the samples can be realized, and finally, the liquids flow into the collector 60 from the outlet valve 50, and the collector 60 can collect the target protein components.

In the present invention, the chromatography units 40 have a plurality of sets, and the chromatography units 40 are arranged in series. The first chromatographic columns 42 of each set of the chromatographic units 40 may be arranged identically or differently. By arranging a plurality of sets of chromatography units 40 in series, the sample can be subjected to chromatography operation in sequence. While the first chromatography column 42 in each chromatography unit 40 may be differently arranged so that different chromatography operations can be performed. The single-column valve 41 may include a plurality of channels and a third selection switch, and by switching of the third selection switch, communication between two channels may be achieved, and two ends of the first chromatography column 41 are respectively connected to two channels of the single-column valve 41, so that by switching of the third selection switch, liquid may or may not flow through the first chromatography column 41.

In another embodiment, the chromatography unit 40 further comprises a multi-position valve and a plurality of second chromatography columns, the multi-position valve being connected to the plurality of second chromatography columns to control the flow of the sample fluid through the predetermined second chromatography columns. For example, a plurality of second chromatography columns may be provided, and the operation of the multi-position valve allows the liquid to selectively flow through one of the second chromatography columns. For the multi-position valve, the multi-position valve disclosed in patent application No. 2020202014584 can be used.

The utility model discloses still include detecting element, detecting element sets up between chromatography unit 40 and outlet valve 50. The detection unit may perform various detections on the liquid output by the chromatography unit 40. The detection unit is an ultraviolet detector 70, a conductivity detector 71 or a PH detector. By these detection units, the liquid output from the chromatography unit 40 can be further detected to determine whether the protein purification satisfies the requirements.

The utility model discloses still include sample liquid bottle 80, balanced liquid bottle 81 and eluent bottle 82, sample liquid bottle 80, balanced liquid bottle 81 and eluent bottle 82 and first inlet 11 one-to-one setting. Thus, different types of liquids can be selectively introduced through the first loading port 11.

The first chromatographic column 42 is an affinity column, a desalting column, an ion exchange column or a gel filtration column. The worker can select different first chromatographic columns 42 and different combinations of chromatographic columns according to the work requirement.

Preferably, the infusion pump 20 has one or more. The number of the first liquid inlet 11 of the inlet valve 10 is 4-6.

It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications can be made without departing from the scope of the invention.

Claims

1. A protein chromatography apparatus, comprising:

an outlet valve connected with the output end of the chromatography unit;

2. The protein chromatography device of claim 1, wherein the chromatography units have multiple sets, the multiple sets being arranged in series.

3. The protein chromatography device of claim 2, wherein the first chromatography column of each set of chromatography units is different.

4. The protein chromatography device of claim 1, wherein the chromatography unit further comprises a plurality of second chromatography columns and a multi-column valve coupled to the plurality of second chromatography columns to control the flow of sample fluid through the predetermined second chromatography columns.

5. The protein chromatography device of claim 1, further comprising a detection unit disposed between the chromatography unit and the outlet valve.

6. The protein chromatography device of claim 5, wherein the detection unit is an ultraviolet detector, a conductivity detector, or a pH detector.

7. The protein chromatography device of claim 1, further comprising a sample liquid bottle, a balance liquid bottle and an elution liquid bottle, wherein the sample liquid bottle, the balance liquid bottle and the elution liquid bottle are arranged in one-to-one correspondence with the first liquid inlet.

8. The protein chromatography device of claim 1, wherein the first chromatography column is an affinity column, a desalting column, an ion exchange column, or a gel filtration column.

9. The protein chromatography device of claim 1, wherein the infusion pump has one or more.

10. The protein chromatography device of claim 1, wherein the first inlet port of the inlet valve is 4-6.