CN214310338U - Multidimensional liquid chromatography separation system based on two-position eight-way valve - Google Patents
Multidimensional liquid chromatography separation system based on two-position eight-way valve Download PDFInfo
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Abstract
The utility model provides a multidimensional liquid chromatography separation system based on two-position eight-way valve, which comprises a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradient pump B, a high performance liquid dilution pump, a gradient mixer A, a gradient mixer B, an injection valve, an enrichment column array A, an enrichment column array B, a fraction collector, a liquid chromatography separation column array, a detector, a two-position eight-way valve and a connecting pipeline; the switching of an upper one-dimensional separation state and a lower one-dimensional separation state is realized through the switching of the two-position eight-way valve, and the three-dimensional or more than three-dimensional chromatographic separation is realized. All-dimensional chromatographic separation columns are selected through a liquid chromatographic separation column array, and full-on-line monitoring and control of multi-dimensional chromatographic separation are realized on the basis of the same gradient elution system and the same detector, so that the cleanness degrees of the enrichment column and the separation column are controllable. The utility model discloses a select different chromatogram stationary phases and mobile phase to make up, realize the high-efficient separation to monomer compound in the high complex system sample of the separation degree of difficulty.
Description
Technical Field
The utility model belongs to the technical field of high performance liquid chromatography separation, a multidimensional liquid chromatography separation system is related to.
Background
With the development of separation technology, the search and separation of components in complex sample systems has become a hot research field. The multidimensional liquid chromatography technology effectively improves the separation degree of the separation of complex sample components by improving the peak capacity, and becomes the development direction of the rapid chromatographic separation technology. The multidimensional liquid chromatography technology is a liquid chromatography combined technology which sequentially injects eluent of a first-dimension chromatographic column of a sample into a subsequent-dimension chromatographic column for further separation. Such separation techniques may employ chromatographic columns of two or more different separation mechanisms to perform orthogonal separation of samples. The most common multidimensional liquid chromatography interface technologies are 3 types: sample loop based interface technology; interface technology based on enrichment columns (also known as trapping columns); interface technology based on stay mode.
At present, a commonly used multi-dimensional liquid chromatography separation system mainly includes a continuous loop switching type two-dimensional liquid chromatography system and a serial mode multi-dimensional liquid chromatography system.
The continuous ring switching type two-dimensional liquid chromatography system has the following operation modes: and a section of sample separated by the first-dimension separation system is delivered to the second-dimension separation system for separation, the first-dimension separation system continues to perform the first-dimension separation, and the steps are repeated in this way to finish all the separations. The continuous ring switching type two-dimensional liquid chromatography system has extremely high separation speed, but the separation of the second-dimensional liquid chromatography is severely restricted by the first-dimensional liquid chromatography, and the application field is limited to a certain extent. In addition, if the continuous ring switching type two-dimensional liquid chromatography system is to realize three-dimensional or higher-dimensional chromatographic separation, cascade connection is required to be carried out to form a cascade parallel multidimensional chromatographic separation system, the cost is high, and the use control is complex.
The serial mode multidimensional liquid chromatography system has the following operation modes: firstly, operating a first-dimension separation system, accurately cutting and enriching the separated sample components in a plurality of enrichment columns in sequence, and starting second-dimension separation after the first-dimension separation is finished; the multi-dimensional chromatographic separation is realized by reciprocating in the way. The serial mode multidimensional liquid phase chromatographic system is represented by a full-automatic high-throughput preparation type separation system sepbox ox series product of Sepiatec GmbH company in Germany, but the product can only carry out two-dimensional chromatographic separation and has limited separation capability.
Chinese patent application CN105938130A discloses a two-position eight-way valve based two-dimensional liquid chromatography separation system, which belongs to a serial mode multi-dimensional liquid chromatography system. The system only has two-dimensional chromatographic separation capability and cannot completely meet the requirements of high-difficulty repeated analysis, separation and preparation of a complex sample system.
SUMMERY OF THE UTILITY MODEL
The utility model aims at further improving the separating power of two-dimensional liquid chromatogram piece-rate system based on two eight logical valves, the messenger possesses three-dimensional or three-dimensional above chromatogram separating power.
In order to achieve the above purpose, the technical scheme of the utility model is that:
a multi-dimensional liquid chromatography separation system comprises a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradient pump B, a diluent pump, a gradient mixer A, a gradient mixer B, a sample injection valve, an enrichment column array A, an enrichment column array B, a fraction collector, a liquid chromatography separation column array, a detector, a two-position eight-way valve and a connecting pipeline. The first position, the second position, the third position, the fourth position, the fifth position, the sixth position, the seventh position and the eighth position of the two-position eight-way valve only represent adjacent relations and do not need to correspond to physical marks of the two-position eight-way valve, and the naming and the sequencing of the number positions are that the sequence is named from any interface of the two-position eight-way valve to the first position in a counterclockwise or clockwise mode. The detector is used for detecting chromatographic signals in the separation process.
The liquid chromatographic separation column array is formed by connecting a plurality of chromatographic separation columns in parallel through a multi-position selection valve, and only one chromatographic separation column can be conducted at the same time; a fixed inlet and a fixed outlet are arranged outside, and at least one bypass is arranged, and the bypass is connected with the separation column in parallel through a multi-position selection valve; when the bypass is conducted, other chromatographic separation columns cannot be conducted, and when other chromatographic separation columns are conducted, the bypass cannot be conducted; the number of chromatographic columns depends on the need, 3 columns being recommended if three-dimensional and 4 columns if four-dimensional.
The enrichment column array A and the enrichment column array B are formed by connecting a plurality of chromatographic enrichment columns in parallel through a multi-position selection valve, and only one enrichment column can be conducted at the same time; at least one bypass is connected with the enrichment column in parallel through a multi-position selection valve; when the bypass is conducted, other enrichment columns cannot be conducted, and when other enrichment columns are conducted, the bypass cannot be conducted; two interfaces are externally arranged and respectively defined as an interface X and an interface Y; the number of enrichment columns is determined as required and is mainly limited by the length of the pipeline and the installation space. A plurality of enrichment column arrays can be connected in series, namely an interface Y of a previous enrichment column array is connected with an interface X of a secondary enrichment column array to form a multi-stage enrichment column array, the operation control is consistent with that of a single-stage enrichment column array, and only one enrichment column can be conducted at the same time; when the multi-stage enrichment column array is in a bypass conduction state, the enrichment column array of each stage is in bypass conduction.
The high-performance liquid chromatography gradient pump A and the high-performance liquid chromatography gradient pump B are connected with an inlet of a gradient mixer A, an outlet of the gradient mixer A is connected with a sample injection valve, and an outlet of the sample injection valve is connected with the number I of the two-position eight-way valve; the second position of the two-position eight-way valve is connected with the interface X of the enrichment column array B, and the interface Y of the enrichment column array B is connected with the sixth position of the two-position eight-way valve; the number fifth of the two-position eight-way valve is connected with an inlet of a liquid chromatography separation column array, an outlet of the liquid chromatography separation column array is connected with a detector, an outlet of the detector is connected with an inlet of a gradient mixer B, a diluent pump is connected with an inlet of the gradient mixer B, and an outlet of the gradient mixer B is connected with the number seventh of the two-position eight-way valve; the eight number position of the two-position eight-way valve is connected with an X interface of the enrichment column array A, and a Y interface of the enrichment column array A is connected with the fourth number position of the two-position eight-way valve; the third position of the two-position eight-way valve is connected with the inlet of the fraction collector.
Based on the pipeline connection mode of the multi-dimensional liquid chromatography separation system, the switching state of the two-position eight-way valve is controlled, the system is switched from the upper one-dimensional separation state to the lower one-dimensional separation state, the circulating chromatography separation function is completed, and the multi-dimensional full-on-line detection chromatography separation function is realized.
The sample injection valve in the multidimensional liquid chromatography separation system can be also connected to a bypass of the enrichment column array A or the enrichment column array B; at the moment, the outlet of the gradient mixer A is connected with the number I of the two-position eight-way valve; the above-mentioned connection changes do not affect the use of the system, but only redefine the dimension of the enrichment columns during control.
The two-position eight-way valve can be one valve or one or more valves and operates according to the switching valve principle of the two-position eight-way valve. The sample injection valve is a sample injection device, and can be a two-position six-way switching sample injection valve or a sample injector; can be other multi-position switching sample loading valves for realizing liquid or solid sample loading; or a chromatographic column for realizing solid-state loading.
The high performance liquid chromatography gradient pump A and the high performance liquid chromatography gradient pump B are both composed of two unit pumps or a multi-element gradient pump. The diluent pump is a high-efficiency liquid phase diluent pump, and is a unit pump or a multi-element pump. The high performance liquid chromatography gradient pump A, the high performance liquid chromatography gradient pump B and the diluent pump, the diluent can be water, salt solution, methanol, acetonitrile, acetone, ethanol or normal alkane solvent, and the eluent can be methanol, acetonitrile, ethanol, water and mixture thereof, normal alkane and other common organic solvents.
The detector is any of various devices for detecting chromatographic signals during separation, including but not limited to ultraviolet detectors, diode array detectors, evaporative light scattering detectors or mass spectrometry detectors, and may be a combination of one or more detectors.
The chromatographic columns of the separation column array, the enrichment column array A and the enrichment column array B can be selected from the same or different fillers, and the fillers can be silica gel, reversed phase silica gel matrix fillers with C18, Xion, C8, CN groups or amino groups, or fillers such as various macroporous adsorption resins, ion exchange resins and the like.
The multi-position switching valve is just one implementation of a column array; when one column in a column array is conductive, the other columns in the column array and the bypass are nonconductive, and when the column array bypass is conductive, the other columns in the column array are nonconductive.
The utility model discloses remain original two dimension liquid chromatography piece-rate system's based on two eight logical valves advantage, compared with original two dimension liquid chromatography piece-rate system based on two eight logical valves, the utility model discloses an innovation point and beneficial effect lie in:
the chromatographic separation capability of the two-dimensional liquid chromatographic separation system based on the two-position eight-way valve is improved to be three-dimensional or higher, and the application range is wider.
Drawings
Fig. 1 is a structure diagram of a pipeline connection in odd-numbered dimension separation states such as the first dimension, the third dimension, etc. of the multi-dimensional liquid chromatography separation system provided by the utility model, and the two-position eight-way valve is in the state a;
fig. 2 is a structural diagram of a pipeline connection in an even-numbered separation state such as the second dimension and the fourth dimension of the multi-dimensional liquid chromatography separation system provided by the present invention, and the two-position eight-way valve is in a B state;
FIG. 3 is a schematic view of a piping connection structure of a liquid chromatography column array;
FIG. 4 is a view showing a structure of a piping connection structure of an enrichment column array A and an enrichment column array B;
FIG. 5(a) is a structure diagram of a sample loading state (LOAD state, A state) pipeline connection structure of a two-position six-way sampling valve, in which a sample is loaded into a quantitative ring, wherein the fourth position is defined as an inlet of the sampling valve, and the fifth position is defined as an outlet of the sampling valve;
FIG. 5(B) is a schematic diagram of a pipeline connection structure in a two-position six-way sampling valve sample loading state (INJECT state, B state), in which a sample is injected into a flow path of a separation system from a quantitative loop for separation, wherein the fourth position is defined as an inlet of the sampling valve, and the fifth position is defined as an outlet of the sampling valve;
fig. 6(a) is a structural diagram of a multidimensional high performance liquid chromatography separation system according to an embodiment of the present invention, wherein the two-position eight-way valve is in a state a;
in fig. 6 (a): 1, a high performance liquid chromatography gradient pump A, 2, a high performance liquid chromatography gradient pump B, 3, a diluent pump, 4, a gradient mixer A, 5, a gradient mixer B, 6, a sample injection valve, 7, 8, a distillate collector, 10, a liquid chromatography separation column array, 11 and 12 two-position eight-way valves;
fig. 6(B) is a structural diagram of the multidimensional high performance liquid chromatography separation system of the embodiment of the present invention, and the two-position eight-way valve is in a state B.
Detailed Description
The following embodiments are merely illustrative of the application of the present invention, and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art without departing from the spirit of the present invention should fall within the protection scope defined by the claims.
A multi-dimensional liquid chromatography separation system comprises a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradient pump B, a diluent pump, a gradient mixer A, a gradient mixer B, a sample injection valve, an enrichment column array A, an enrichment column array B, a fraction collector, a liquid chromatography separation column array, a detector, a two-position eight-way valve and a connecting pipeline. Wherein, the diluent pump is a high-efficiency liquid phase diluent pump.
The high-efficiency liquid chromatography gradient pump A and the high-efficiency liquid chromatography gradient pump B are connected with inlets of the gradient mixer A, outlets of the gradient mixer A are connected with a sample injection valve, outlets of the sample injection valve are connected with the first position of a two-position eight-way valve, the second position of the two-position eight-way valve is connected with an X interface of an enrichment column array B, a Y interface of the enrichment column array B is connected with the sixth position of the two-position eight-way valve, the fifth position of the two-position eight-way valve is connected with an inlet of a liquid chromatography separation column array, outlets of the liquid chromatography separation column array are connected with a detector, an outlet of the detector is connected with an inlet of the gradient mixer B, a diluent pump is connected with an inlet of the gradient mixer B, and an outlet of the gradient mixer B is connected with the seventh position of the two-position eight-way valve; the eight number position of the two-position eight-way valve is connected with an X interface of the enrichment column array A, and a Y interface of the enrichment column array A is connected with the fourth number position of the two-position eight-way valve; the third position of the two-position eight-way valve is connected with the inlet of the fraction collector. The first position, the second position, the third position, the fourth position, the fifth position, the sixth position, the seventh position and the eighth position of the two-position eight-way valve only represent adjacent relations and do not need to correspond to physical marks of the two-position eight-way valve.
In fig. 1, the two-position eight-way valve is in a state a, at this time, a high performance liquid chromatography gradient pump a, a high performance liquid chromatography gradient pump B and a gradient mixer a form a chromatography separation gradient elution mobile phase supply system, an outlet of the gradient mixer a is connected with a sample injection valve, and an outlet of the sample injection valve is connected with the first position of the two-position eight-way valve; the first position and the second position of the two-position eight-way valve are communicated and are connected with the interface X of the enrichment column array B (at the moment, the interface X of the enrichment column array B is the inlet of the enrichment column array B); the interface Y of the enrichment column array B (the interface Y of the enrichment column array B is the outlet of the enrichment column array B at the moment) is connected with the sixth position of the two-position eight-way valve and is connected with the inlet of the liquid chromatography separation column array through the fifth position of the two-position eight-way valve; selecting any chromatographic column in the separation column array for separation; an outlet of the separation column array is connected with a detector, the detector detects chromatographic signals, an outlet of the detector is connected with an inlet of a gradient mixer B, a diluent pump is connected with an inlet of the gradient mixer B, a sample flows out after the column is diluted by the gradient mixer B, and an outlet of the gradient mixer B is connected with the position of the two-position eight-way valve; the number of the two-position eight-way valve is communicated with the number of the tool bit; the position of the eight symbol of the two-position eight-way valve is connected with an interface X of the enrichment column array A (the interface X of the enrichment column array A is the inlet of the enrichment column array A at the moment), and an interface Y of the enrichment column array A (the interface Y of the enrichment column array A is the outlet of the enrichment column array A at the moment) is connected with the position of the four symbol of the two-position eight-way valve, so that enrichment of a separation sample is realized; the No. four position of the two-position eight-way valve is communicated with the No. three position of the two-position eight-way valve; and the third position of the two-position eight-way valve is connected with the inlet of the fraction collector to realize sample collection.
In fig. 2, the two-position eight-way valve is in a state of B, at this time, the high performance liquid chromatography gradient pump a, the high performance liquid chromatography gradient pump B and the gradient mixer a form a chromatography separation gradient elution mobile phase supply system, an outlet of the gradient mixer a is connected with a sample injection valve, an outlet of the sample injection valve is connected with the position I of the two-position eight-way valve, and the position I of the two-position eight-way valve is communicated with the position I; the eighth bit of the two-bit eight-way valve is connected with an interface X of the enrichment column array A (the interface X of the enrichment column array A is an inlet of the enrichment column array A at the moment); the interface Y of the enrichment column array A (the interface Y of the enrichment column array B is the outlet of the enrichment column array A at the moment) is connected with the No. four position of the two-position eight-way valve; the No. four position and the No. five position of the two-position eight-way valve are communicated, and the No. five position of the two-position eight-way valve is connected with an inlet of the liquid chromatography separation column array; selecting any chromatographic column in the separation column array for separation; an outlet of the separation column array is connected with a detector, the detector detects chromatographic signals, an outlet of the detector is connected with an inlet of a gradient mixer B, a diluent pump is connected with an inlet of the gradient mixer B, a sample flows out after the column is diluted by the gradient mixer B, and an outlet of the gradient mixer B is connected with the position of the two-position eight-way valve; the seventh position of the two-position eight-way valve is communicated with the sixth position; the sixth position of the two-position eight-way valve is connected with the interface Y of the enrichment column array B (the interface Y of the enrichment column array B is the inlet of the enrichment column array B); an interface X of the enrichment column array B (the interface X of the enrichment column array A is an outlet of the enrichment column array A at the moment) is connected with the No. two position of the two-position eight-way valve, so that enrichment of the separated sample is realized; the No. two position and the No. three position of the two-position eight-way valve are communicated; and the third position of the two-position eight-way valve is connected with the inlet of the fraction collector to realize sample collection.
Example (b): multidimensional liquid chromatography separation system structure
In the embodiment, the enrichment column array B comprises 9 enrichment columns which are sequentially numbered as the 1 st enrichment column, the 2 nd enrichment column and the like of the enrichment column array B, and the last enrichment column is numbered as the 9 th enrichment column of the enrichment column array B; the enrichment column array A is a two-stage enrichment column array, each stage of the enrichment column array is provided with 9 enrichment columns, namely the enrichment column array A is 18 enrichment columns which are sequentially numbered as the 1 st enrichment column, the 2 nd enrichment column and the like of the enrichment column array A, and the last enrichment column is numbered as the 18 th enrichment column of the enrichment column array A; the liquid chromatography separation column array comprises 5 separation columns which are sequentially numbered as a 1 st separation column, a 2 nd separation column and the like, and the last separation column is a 5 th separation column; the two-position eight-way valve in fig. 6(a) is in the a state, and the two-position eight-way valve in fig. 6(B) is in the B state.
The following is the control of the four-dimensional separation process of the structure of the multidimensional high performance liquid chromatography separation system:
the operation mode of the multidimensional liquid chromatographic separation system mainly comprises two modes, wherein the first mode is a plurality of cycles of separation-enrichment and is finally finished by separation; the second is a number of cycles of enrichment-separation, eventually also ending with separation. A four-dimensional liquid chromatography separation control process is briefly described below.
Firstly, cleaning an enrichment column and a separation column; and sequentially switching each enrichment column and each separation column into the flow path, and observing signals of the detector to judge the cleaning effect.
Controlling the first-dimension separation process: the two-position eight-way valve is in the state A, and is shown in figure 1; the enrichment column array B is in a bypass state; loading a sample into a dosing ring on a sample injection valve; selecting a first dimension chromatographic separation column, e.g., the 1 st separation column, which is manually turned on; when the injection valve is switched to an INJECT state, starting first-dimension separation; under the assistance of a diluent pump, sequentially enriching fractions by adopting enrichment columns from 1 st to 9 th of an enrichment column array A according to sample properties and detection signals, and reserving enrichment columns from 10 th to 18 th of the enrichment column array A for third-dimensional separation; repeating the steps until enough compounds exist in the 1 st to the 9 th enrichment columns of the enrichment column array A, and switching to the control of a second-dimensional separation process; if the second dimension separation is not needed, the enrichment column array A is always in a bypass state, and a plurality of fractions are directly collected by using a fraction collector.
And (3) controlling a second-dimension separation process: after the control of the first dimension separation process is finished, the sample injection valve should be switched to the LOAD state, and the two-position eight-way valve is switched to the B state, which is shown in figure 2; selecting a second dimension chromatographic separation column, e.g., the 2 nd separation column, which is manually turned on; selecting one enrichment column from the 1 st to the 9 th enrichment columns of the enrichment column array A as a sample column for second-dimensional separation; when the enrichment column is conducted, a second dimension separation process is started; under the assistance of a diluent pump, sequentially switching the fractions to enrichment columns 1 to 9 of the enrichment column array B for enrichment according to the sample properties and detection signals; if the third-dimensional separation is not needed, sequentially eluting the 1 st to 9 th enrichment columns of the enrichment column array B, and directly collecting a plurality of fractions by using a fraction collector; repeating the steps to finish the second dimension separation;
and (3) controlling a third-dimensional separation process: after the control of the second dimension separation process is finished, the two-position eight-way valve is switched to the state A, which is shown in figure 1; the sample injection valve keeps a LOAD state; selecting a third dimension chromatographic separation column, e.g., the 3 rd separation column, which is manually turned on; selecting one enrichment column from the 1 st to the 9 th enrichment columns of the enrichment column array B as a sample column for third-dimensional separation; when the enrichment column is conducted, a third-dimensional separation process is started; if the fourth dimension separation is needed, under the assistance of a diluent pump, sequentially switching the fractions into enrichment columns from 10 th to 18 th of the enrichment column array A according to the sample properties and detection signals for enrichment, and cutting the fractions into 9 parts; if the fourth dimension separation is not needed, sequentially eluting and separating the 1 st to 9 th enrichment columns of the enrichment column array B, and directly collecting a plurality of fractions by using a fraction collector; and repeating the steps to finish the third-dimensional separation.
And controlling a fourth dimension separation process: after the control of the third separation process is finished, the two-position eight-way valve is switched to the state B, which is shown in fig. 2; the sample injection valve keeps a LOAD state; selecting a fourth dimension chromatographic separation column, e.g., a 4 th separation column, which is manually turned on; selecting one enrichment column from 10 th to 18 th enrichment columns of the enrichment column array A as a sample column for fourth-dimensional separation; when the enrichment column is conducted, the fourth dimension separation process is started; under the action of gradient eluent, eluting the compound in the enrichment column as a sample column, and performing fourth-dimension separation under the action of a 4 th separation column; collecting a plurality of fractions by using a fraction collector; and repeating the steps to finish the fourth dimension separation.
Claims (7)
1. A multidimensional liquid chromatography separation system is characterized by comprising a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradient pump B, a diluent pump, a gradient mixer A, a gradient mixer B, a sample injection valve, an enrichment column array A, an enrichment column array B, a fraction collector, a liquid chromatography separation column array, a detector, a two-position eight-way valve and a connecting pipeline; the first position, the second position, the third position, the fourth position, the fifth position, the sixth position, the seventh position and the eighth position of the two-position eight-way valve only represent adjacent relations and do not need to correspond to physical marks of the two-position eight-way valve, and the naming and the sequencing of the number positions are that the sequence naming is started from the first position counterclockwise or clockwise from any interface of the two-position eight-way valve; the detector is used for detecting chromatographic signals in the separation process; the sample injection valve is used for sample injection;
the liquid chromatographic separation column array is formed by connecting a plurality of chromatographic separation columns in parallel through a multi-position selection valve, and only one chromatographic separation column can be conducted at the same time; a fixed inlet and a fixed outlet are arranged outwards, and at least one bypass is arranged, and the bypass is connected with the separation column in parallel through a multi-position selection valve; when the bypass is conducted, other chromatographic separation columns cannot be conducted, and when other chromatographic separation columns are conducted, the bypass cannot be conducted;
the enrichment column array A and the enrichment column array B are formed by connecting a plurality of chromatographic enrichment columns in parallel through a multi-position selection valve, and only one enrichment column can be conducted at the same time; at least one bypass is connected with the enrichment column in parallel through a multi-position selection valve; when the bypass is conducted, other enrichment columns cannot be conducted, and when other enrichment columns are conducted, the bypass cannot be conducted; two interfaces are externally arranged and respectively defined as an interface X and an interface Y;
the high-performance liquid chromatography gradient pump A and the high-performance liquid chromatography gradient pump B are connected with an inlet of a gradient mixer A, an outlet of the gradient mixer A is connected with an inlet of a sample injection valve, an outlet of the sample injection valve is connected with the number I of a two-position eight-way valve, the number II of the two-position eight-way valve is connected with an interface X of an enrichment column array B, an interface Y of the enrichment column array B is connected with the number II of the two-position eight-way valve, the number III of the two-position eight-way valve is connected with an inlet of a liquid chromatography separation column array, an outlet of the liquid chromatography separation column array is connected with a detector, an outlet of the detector is connected with an inlet of the gradient mixer B, a diluent pump is connected with an inlet of the gradient mixer B, and an outlet of the gradient mixer B is connected with the number III of the two-position eight-way valve; the eighth position of the two-position eight-way valve is connected with the interface X of the enrichment column array A, and the interface Y of the enrichment column array A is connected with the fourth position of the two-position eight-way valve; the third position of the two-position eight-way valve is connected with the inlet of the fraction collector;
by controlling the switching state of the two-position eight-way valve, the system is switched from the upper one-dimensional separation state to the lower one-dimensional separation state, the circulating chromatographic function is completed, and the chromatographic separation function of multi-dimensional full-on-line detection is realized.
2. The multi-dimensional liquid chromatography separation system of claim 1, wherein the sample injection valve is connected in a bypass of the enrichment column array A or the enrichment column array B, and the outlet of the gradient mixer A is connected with the first position of the two-position eight-way valve.
3. The multi-dimensional liquid chromatography separation system of claim 1 or 2, wherein the plurality of enrichment column arrays are connected in series to form a multi-stage enrichment column array, operation control is consistent with that of a single-stage enrichment column array, and only one enrichment column can be conducted at the same time; when the multi-stage enrichment column array is in a bypass conduction state, the enrichment column array of each stage is in bypass conduction.
4. A multi-dimensional liquid chromatography separation system according to claim 1 or 2, wherein said hplc gradient pump a and said hplc gradient pump B are each comprised of two unit pumps, or are comprised of one multi-unit gradient pump; the diluent pump is a unit pump or a multi-element pump.
5. A multi-dimensional liquid chromatography separation system according to claim 3, wherein said hplc gradient pump a and said hplc gradient pump B are each comprised of two unit pumps, or are comprised of one multi-unit gradient pump; the diluent pump is a unit pump or a multi-element pump.
6. A multi-dimensional liquid chromatography separation system according to claim 1 or 2, wherein the detector is a device for detecting chromatographic signals during separation, comprising an ultraviolet detector, a diode array detector, an evaporative light scattering detector or a mass spectrometry detector, comprising a combined detection system of a plurality of detectors.
7. A multi-dimensional liquid chromatography separation system according to claim 5 wherein the detector is a device for detecting chromatographic signals during separation, comprising an ultraviolet detector, a diode array detector, an evaporative light scattering detector or a mass spectrometry detector, comprising a combined detection system of multiple detectors.
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| CN109557221A (en) * | 2019-01-10 | 2019-04-02 | 李宜珊 | A kind of multidimensional liquid chromatographic separation system based on two eight ways valves |
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