CN211798964U - Multidimensional liquid chromatography separation system based on two-position ten-way valve - Google Patents
Multidimensional liquid chromatography separation system based on two-position ten-way valve Download PDFInfo
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Abstract
The utility model provides a multidimensional liquid chromatography separation system based on two ten-way valves, including high performance liquid chromatography gradient pump A, high performance liquid chromatography gradient pump B, high performance liquid diluent pump, gradient mixer A, gradient mixer B, sampling valve, enrichment column array A, enrichment column array B, fraction collector, liquid chromatography separation column array, detector, two ten-way valves and connecting line; the switching of an upper one-dimensional separation state and a lower one-dimensional separation state is realized through the switching of the two-position ten-way valve, and the three-dimensional or more than three-dimensional chromatographic separation is realized. Based on the same gradient elution system and the same detector, full online monitoring and control of multi-dimensional chromatographic separation are realized, and the cleanness of the enrichment column and the separation column is controllable. The utility model discloses a select different chromatogram stationary phases and mobile phase to make up, realize the high-efficient separation to monomer compound in the high complex system sample of the separation degree of difficulty.
Description
Technical Field
The utility model belongs to the technical field of high performance liquid chromatography separation, a multidimensional liquid chromatography separation system is related to.
Background
With the development of separation technology, the search and separation of components in complex sample systems has become a hot research field. The multidimensional liquid chromatography technology effectively improves the separation degree of the separation of complex sample components by improving the peak capacity, and becomes the development direction of the rapid chromatographic separation technology. The multidimensional liquid chromatography technology is a liquid chromatography combined technology which sequentially injects eluent of a first-dimension chromatographic column of a sample into a subsequent-dimension chromatographic column for further separation. Such separation techniques may employ chromatographic columns of two or more different separation mechanisms to perform orthogonal separation of samples. The most common multidimensional liquid chromatography interface technologies are 3 types: sample loop based interface technology; interface technology based on enrichment columns (also known as trapping columns); interface technology based on stay mode.
At present, a commonly used multi-dimensional liquid chromatography separation system mainly includes a continuous loop switching type two-dimensional liquid chromatography system and a serial mode multi-dimensional liquid chromatography system.
The continuous ring switching type two-dimensional liquid chromatography system has the following operation modes: and a section of sample separated by the first-dimension separation system is delivered to the second-dimension separation system for separation, the first-dimension separation system continues to perform the first-dimension separation, and the steps are repeated in this way to finish all the separations. The continuous ring switching type two-dimensional liquid chromatography system has extremely high separation speed, but the separation of the second-dimensional liquid chromatography is severely restricted by the first-dimensional liquid chromatography, and the application field is limited to a certain extent. In addition, if the continuous ring switching type two-dimensional liquid chromatography system is to realize three-dimensional or higher-dimensional chromatographic separation, cascade connection is required to be carried out to form a cascade parallel multidimensional chromatographic separation system, the cost is high, and the use control is complex.
The serial mode multidimensional liquid chromatography system has the following operation modes: firstly, operating a first-dimension separation system, accurately cutting and enriching the separated sample components in a plurality of enrichment columns in sequence, and starting second-dimension separation after the first-dimension separation is finished; the multi-dimensional chromatographic separation is realized by reciprocating in the way. The serial mode multi-dimensional liquid chromatography system is represented by a full-automatic high-throughput preparation type separation system sepbox ox series product of Sepiatec GmbH company, but the product can only carry out two-dimensional chromatographic separation and has limited separation capacity.
Chinese patent application CN108037233A discloses a two-position ten-way valve based multi-dimensional liquid chromatography separation system, which belongs to a serial mode multi-dimensional liquid chromatography system, can provide three-dimensional or above liquid chromatography separation capability, and is convenient for realizing high-efficiency preparation of monomeric compounds. Chinese patent applications CN109557219A and CN109655561A disclose a two-position ten-way valve based multi-dimensional liquid chromatography separation system with reverse elution function of enrichment column, respectively, which can save separation time and mobile phase to a certain extent while providing three-dimensional or more than three-dimensional liquid chromatography separation capability.
SUMMERY OF THE UTILITY MODEL
The utility model aims at enriching the post reverse elution to there is certain problem under certain circumstances, need be on-the-spot change its enriching post forward elution into fast, provide a low-cost multidimensional liquid chromatography piece-rate system of being convenient for quick switching enriching post elution direction.
In order to achieve the above purpose, the technical scheme of the utility model is that:
a multi-dimensional liquid chromatography separation system comprises a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradient pump B, a diluent pump, a gradient mixer A, a gradient mixer B, a sample injection valve, an enrichment column array A, an enrichment column array B, a fraction collector, a liquid chromatography separation column array, a detector, a two-position ten-way valve and a connecting pipeline. The first position, the second position, the third position, the fourth position, the fifth position, the sixth position, the seventh position, the eighth position, the ninth position and the third position of the two-position ten-way valve only represent adjacent relations and do not correspond to physical marks of the two-position ten-way valve, and the number naming and the sequencing are that the naming is started from any interface of the two-position ten-way valve according to the anticlockwise or clockwise sequencing from the first position. The detector is used for detecting chromatographic signals in the separation process.
The liquid chromatographic separation column array is formed by connecting a plurality of chromatographic separation columns in parallel through a multi-position selection valve, and only one chromatographic separation column can be conducted at the same time; a fixed inlet and a fixed outlet are arranged outside, and at least one bypass is arranged, and the bypass is connected with the separation column in parallel through a multi-position selection valve; when the bypass is conducted, other chromatographic separation columns cannot be conducted, and when other chromatographic separation columns are conducted, the bypass cannot be conducted; the number of chromatographic columns depends on the need, 3 columns being recommended if three-dimensional and 4 columns if four-dimensional.
The enrichment column array A and the enrichment column array B are formed by connecting a plurality of chromatographic enrichment columns in parallel through a multi-position selection valve, and only one enrichment column can be conducted at the same time; at least one bypass is connected with the enrichment column in parallel through a multi-position selection valve; when the bypass is conducted, other enrichment columns cannot be conducted, and when other enrichment columns are conducted, the bypass cannot be conducted; two interfaces are externally arranged and respectively defined as an interface X and an interface Y; the number of enrichment columns is determined as required and is mainly limited by the length of the pipeline and the installation space. A plurality of enrichment column arrays can be connected in series, namely an interface Y of a previous enrichment column array is connected with an interface X of a secondary enrichment column array to form a multi-stage enrichment column array, the operation control is consistent with that of a single-stage enrichment column array, and only one enrichment column can be conducted at the same time; when the multi-stage enrichment column array is in a bypass conduction state, the enrichment column array of each stage is in bypass conduction.
The high performance liquid chromatography gradient pump A and the high performance liquid chromatography gradient pump B are connected with an inlet of a gradient mixer A, an outlet of the gradient mixer A is connected with a sample injection valve, an outlet of the sample injection valve is connected with the number I of a two-position ten-way valve, the number III of the two-position ten-way valve is connected with an interface X of an enrichment column array B, an interface Y of the enrichment column array B is connected with the number III of the two-position ten-way valve, the number II of the two-position ten-way valve is connected with the number III of the two-position ten-way valve, the number III of the two-position ten-way valve is connected with an inlet of a liquid chromatography separation column array, an outlet of the liquid chromatography separation column array is connected with a detector, an outlet of the detector is connected with an inlet of the gradient mixer B, a dilution liquid pump is connected with an inlet of the gradient mixer B, an outlet of the gradient mixer B is connected with the number III of the two-position ten-way valve, the Y interface of the enrichment column array A is connected with the fifth position of the two-position ten-way valve, and the fourth position of the two-position ten-way valve is connected with the inlet of the fraction collector.
Based on the pipeline connection mode of the multi-dimensional liquid chromatography separation system, the switching state of the two-position ten-way valve is controlled to realize the conversion of the system from the upper one-dimensional separation state to the lower one-dimensional separation state, so that the circulating chromatography function is completed, and the chromatography separation function of multi-dimensional full-on-line detection is realized.
The sample injection valve in the multidimensional liquid chromatography separation system can be also connected to a bypass of the enrichment column array A or the enrichment column array B; at the moment, the outlet of the gradient mixer A is connected with the number I of the two-position ten-way valve; the above-mentioned connection changes do not affect the use of the system, but only redefine the dimension of the enrichment columns during control.
The two-position ten-way valve can be one valve or one or more valves and operates according to the switching valve principle of the two-position ten-way valve. The sample injection valve is a sample injection device, and can be a two-position six-way switching sample injection valve or a sample injector; can be other multi-position switching sample loading valves for realizing liquid or solid sample loading; or a chromatographic column for realizing solid-state loading.
The high performance liquid chromatography gradient pump A and the high performance liquid chromatography gradient pump B are both composed of two unit pumps or a multi-element gradient pump. The diluent pump is a high-efficiency liquid phase diluent pump, and is a unit pump or a multi-element pump. The high performance liquid chromatography gradient pump A, the high performance liquid chromatography gradient pump B and the diluent pump, the diluent can be water, salt solution, methanol, acetonitrile, acetone, ethanol or normal alkane solvent, and the eluent can be methanol, acetonitrile, ethanol, water and mixture thereof, normal alkane and other common organic solvents.
The detector is any of various devices for detecting chromatographic signals during separation, including but not limited to ultraviolet detectors, diode array detectors, evaporative light scattering detectors or mass spectrometry detectors, and may be a combination of one or more detectors.
The chromatographic columns of the separation column array, the enrichment column array A and the enrichment column array B can be selected from the same or different fillers, and the fillers can be silica gel, reversed phase silica gel matrix fillers with C18, Xion, C8, CN groups or amino groups, or fillers such as various macroporous adsorption resins, ion exchange resins and the like.
The multi-position switching valve is just one implementation of a column array; when one column in a column array is conductive, the other columns in the column array and the bypass are nonconductive, and when the column array bypass is conductive, the other columns in the column array are nonconductive.
The utility model discloses an innovation point lies in with beneficial effect:
need not increase cost such as flow path direction switching valve, use with the system architecture cooperation among the chinese patent application CN109655561A, only change the utility model provides a be connected of these two ports in the position nine on the two ten logical valves in the multi-dimensional liquid chromatography piece-rate system and the position four, can change enrichment column array a, enrichment column array B's elution mode, be convenient for satisfy various needs in practical application.
Drawings
Fig. 1 is a structure diagram of a pipeline connection in odd-numbered dimension separation states such as the first dimension, the third dimension, etc. of the multi-dimensional liquid chromatography separation system provided by the utility model, and the two-position ten-way valve is in the state a;
fig. 2 is a structural diagram of a pipeline connection in an even-numbered separation state such as the second dimension and the fourth dimension of the multi-dimensional liquid chromatography separation system provided by the utility model, and the two-position ten-way valve is in a state B;
FIG. 3 is a schematic view of a piping connection structure of a liquid chromatography column array;
FIG. 4 is a view showing a structure of a piping connection structure of an enrichment column array A and an enrichment column array B;
FIG. 5(a) is a structure diagram of a sample loading state (LOAD state, A state) pipeline connection structure of a two-position six-way sampling valve, in which a sample is loaded into a quantitative ring, wherein the fourth position is defined as an inlet of the sampling valve, and the fifth position is defined as an outlet of the sampling valve;
FIG. 5(B) is a schematic diagram of a pipeline connection structure in a two-position six-way sampling valve sample loading state (INJECT state, B state), in which a sample is injected into a flow path of a separation system from a quantitative loop for separation, wherein the fourth position is defined as an inlet of the sampling valve, and the fifth position is defined as an outlet of the sampling valve;
fig. 6(a) is a structural diagram of a multidimensional high performance liquid chromatography separation system according to an embodiment of the present invention, wherein the two-position ten-way valve is in a state a;
in fig. 6 (a): 1, a high performance liquid chromatography gradient pump A, 2, a high performance liquid chromatography gradient pump B, 3, a diluent pump, 4, a gradient mixer A, 5, a gradient mixer B, 6, a sample injection valve, 7, 8, a fraction collector, 10, a liquid chromatography separation column array, 11 and 12 two-position ten-way valves;
fig. 6(B) is a structural diagram of the multidimensional high performance liquid chromatography separation system of the embodiment of the present invention, and the two-position ten-way valve is in a state B.
Detailed Description
The following embodiments are merely illustrative of the application of the present invention, and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art without departing from the spirit of the present invention should fall within the protection scope defined by the claims.
A multi-dimensional liquid chromatography separation system comprises a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradient pump B, a diluent pump, a gradient mixer A, a gradient mixer B, a sample injection valve, an enrichment column array A, an enrichment column array B, a fraction collector, a liquid chromatography separation column array, a detector, a two-position ten-way valve and a connecting pipeline. Wherein, the diluent pump is a high-efficiency liquid phase diluent pump.
The high performance liquid chromatography gradient pump A and the high performance liquid chromatography gradient pump B are connected with an inlet of a gradient mixer A, an outlet of the gradient mixer A is connected with a sample injection valve, an outlet of the sample injection valve is connected with the number I of a two-position ten-way valve, the number III of the two-position ten-way valve is connected with an X interface of an enrichment column array B, a Y interface of the enrichment column array B is connected with the number III of the two-position ten-way valve, the number II of the two-position ten-way valve is connected with the number III of the two-position ten-way valve, the number III of the two-position ten-way valve is connected with an inlet of a liquid chromatography separation column array, an outlet of the liquid chromatography separation column array is connected with a detector, an outlet of the detector is connected with an inlet of the gradient mixer B, a dilution liquid pump is connected with an inlet of the gradient mixer B, an outlet of the gradient mixer B is connected with the number III of the two-position ten-way valve, the Y interface of the enrichment column array A is connected with the fifth position of the two-position ten-way valve, and the fourth position of the two-position ten-way valve is connected with the inlet of the fraction collector. The first, second, third, fourth, fifth, sixth, seventh, ninth and eighth bit of the two-position ten-way valve only represent adjacent relation, and do not need to correspond to the physical mark of the two-position ten-way valve.
In fig. 1, the two-position ten-way valve is in a state a, at this time, a high performance liquid chromatography gradient pump a, a high performance liquid chromatography gradient pump B and a gradient mixer a form a chromatography separation gradient elution mobile phase supply system, an outlet of the gradient mixer a is connected with a sample injection valve, and an outlet of the sample injection valve is connected with the first position of the two-position ten-way valve; the number one of the two-position ten-way valve is communicated with the number one of the red body and is connected with an interface X of the enrichment column array B (the interface X of the enrichment column array B is the inlet of the enrichment column array B); the interface Y of the enrichment column array B (the interface Y of the enrichment column array B is the outlet) is connected with the third position of the two-position ten-way valve, and the third position of the two-position ten-way valve is communicated with the second position; the No. of the two-position ten-way valve is connected with the No. of the two-position ten-way valve, and the No. of the two-position ten-way valve is communicated with the No. of the two-position ten-way valve; the sixth position of the two-position ten-way valve is connected with the inlet of the liquid chromatographic separation column array; selecting any chromatographic column in the separation column array for separation; the outlet of the separation column array is connected with a detector, the detector detects chromatographic signals, the outlet of the detector is connected with the inlet of a gradient mixer B, a diluent pump is connected with the inlet of the gradient mixer B, a sample flows out after the column is diluted by the gradient mixer B, and the outlet of the gradient mixer B is connected with the position ninthly of the two-position ten-way valve; the ninthly position of the two-position ten-way valve is communicated with the position # viii; the position of the # V of the two-position ten-way valve is connected with an interface X of the enrichment column array A (the interface X of the enrichment column array B is the inlet of the enrichment column array A at the moment), and an interface Y of the enrichment column array A (the interface Y of the enrichment column array A is the outlet of the enrichment column array A at the moment) is connected with the position of the # V of the two-position ten-way valve, so that enrichment of a separated sample is realized; and the No. fifth position and the No. fourth position of the two-position ten-way valve are communicated, and the No. fourth position of the two-position ten-way valve is connected with an inlet of a fraction collector, so that sample collection is realized.
In fig. 2, the two-position ten-way valve is in a state of B, at this time, the high performance liquid chromatography gradient pump a, the high performance liquid chromatography gradient pump B and the gradient mixer a form a chromatography separation gradient elution mobile phase supply system, an outlet of the gradient mixer a is connected with a sample injection valve, an outlet of the sample injection valve is connected with the position I of the two-position ten-way valve, and the position I and the position II of the two-position ten-way valve are communicated; the No. II position of the two-position ten-way valve is connected with the No. III position, and the No. III position of the two-position ten-way valve is communicated with the No. III position; the eighth bit of the two-bit ten-way valve is connected with an interface X of the enrichment column array A (the interface X of the enrichment column array A is an inlet of the enrichment column array A at the moment); the interface Y of the enrichment column array A (the interface Y of the enrichment column array A is the outlet) is connected with the fifth position of the two-position ten-way valve; the fifth position of the two-position ten-way valve is communicated with the sixth position, and the sixth position of the two-position ten-way valve is connected with the inlet of the liquid chromatography separation column array; selecting any chromatographic column in the separation column array for separation; the outlet of the separation column array is connected with a detector, the detector detects chromatographic signals, the outlet of the detector is connected with the inlet of a gradient mixer B, a diluent pump is connected with the inlet of the gradient mixer B, a sample flows out after the column is diluted by the gradient mixer B, and the outlet of the gradient mixer B is connected with the position ninthly of the two-position ten-way valve; the ninthly position of the two-position ten-way valve is communicated with the position on the hole; the position of the wave depth of the two-position ten-way valve is connected with an interface X of the enrichment column array B (the interface X of the enrichment column array B is the inlet of the enrichment column array B); an interface Y of the enrichment column array B (the interface Y of the enrichment column array B is an outlet of the enrichment column array B at the moment) is connected with a No. three position of the two-position ten-way valve, so that enrichment of the separated sample is realized; the third position and the fourth position of the two-position ten-way valve are communicated; and the position IV of the two-position ten-way valve is connected with the inlet of the fraction collector to realize sample collection.
Example (b): three-dimensional high performance liquid chromatography separation system structure
In the embodiment, the enrichment column array B comprises 9 enrichment columns which are sequentially numbered as the 1 st enrichment column, the 2 nd enrichment column and the like of the enrichment column array B, and the last enrichment column is numbered as the 9 th enrichment column of the enrichment column array B; the enrichment column array A is a two-stage enrichment column array, each stage of the enrichment column array is provided with 9 enrichment columns, namely the enrichment column array A is 18 enrichment columns which are sequentially numbered as the 1 st enrichment column, the 2 nd enrichment column and the like of the enrichment column array A, and the last enrichment column is numbered as the 18 th enrichment column of the enrichment column array A; the liquid chromatography separation column array comprises 5 separation columns which are sequentially numbered as a 1 st separation column, a 2 nd separation column and the like, and the last separation column is a 5 th separation column; the two-position ten-way valve in fig. 6(a) is in the a state, and the two-position ten-way valve in fig. 6(B) is in the B state.
The following is the three-dimensional separation process control of the above three-dimensional high performance liquid chromatography separation system structure:
the operation mode of the three-dimensional liquid chromatography separation system mainly comprises two modes, wherein the first mode is a plurality of cycles of separation-enrichment, and the separation is finally finished; the second is a number of cycles of enrichment-separation, eventually also ending with separation. A brief description of a three-dimensional liquid chromatography separation control process follows.
Firstly, cleaning an enrichment column and a separation column; and sequentially switching each enrichment column and each separation column into the flow path, and observing signals of the detector to judge the cleaning effect.
Controlling the first-dimension separation process: the two-position ten-way valve is in the state A, and is shown in figure 1; the enrichment column array B is in a bypass state; loading a sample into a dosing ring on a sample injection valve; selecting a first dimension chromatographic separation column, e.g., the 1 st separation column, which is manually turned on; when the injection valve is switched to an INJECT state, starting first-dimension separation; under the assistance of a diluent pump, sequentially enriching fractions in enrichment columns from 1 st to 18 th of the enrichment column array A according to the properties of the sample and detection signals; repeating the steps until enough compounds exist in the 1 st to the 18 th enrichment columns of the enrichment column array A, and switching to the control of a second-dimensional separation process; if the second dimension separation is not needed, the enrichment column array A is always in a bypass state, and a plurality of fractions are directly collected by using a fraction collector.
And (3) controlling a second-dimension separation process: after the control of the first dimension separation process is finished, the sample injection valve should be switched to the LOAD state, and the two-position ten-way valve is switched to the B state, which is shown in figure 2; selecting a second dimension chromatographic separation column, e.g., the 2 nd separation column, which is manually turned on; selecting one enrichment column from enrichment columns 1 to 18 of the enrichment column array A as a sample column for a second-dimensional separation; when the enrichment column is conducted, a second dimension separation process is started; under the assistance of a diluent pump, sequentially switching the fractions to enrichment columns 1 to 9 of the enrichment column array B for enrichment according to the sample properties and detection signals; if the third-dimensional separation is not needed, sequentially eluting the 1 st to 9 th enrichment columns of the enrichment column array B, and directly collecting a plurality of fractions by using a fraction collector; repeating the steps to finish the second dimension separation;
and (3) controlling a third-dimensional separation process: after the control of the second dimension separation process is finished, the two-position ten-way valve is switched to the state A, which is shown in figure 1; the sample injection valve keeps a LOAD state; the enrichment column array A is in a bypass state; selecting a third dimension chromatographic separation column, e.g., the 3 rd separation column, which is manually turned on; selecting one enrichment column from the 1 st to the 9 th enrichment columns of the enrichment column array B as a sample column for third-dimensional separation; when the enrichment column is conducted, a third-dimensional separation process is started; collecting a plurality of separated fractions by using a fraction collector; and repeating the steps to finish the third-dimensional separation.
Claims (10)
1. A multi-dimensional liquid chromatography separation system is characterized by comprising a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradient pump B, a diluent pump, a gradient mixer A, a gradient mixer B, a sample injection valve, an enrichment column array A, an enrichment column array B, a fraction collector, a liquid chromatography separation column array, a detector, a two-position ten-way valve and a connecting pipeline; the first position, the second position, the third position, the fourth position, the fifth position, the sixth position, the seventh position, the eighth position, the ninth position and the third position of the two-position ten-way valve only represent adjacent relations and do not correspond to physical marks of the two-position ten-way valve, and the number naming and the sequencing of the number positions are that the naming is started from any interface of the two-position ten-way valve according to the anticlockwise or clockwise sequencing from the first position; the detector is used for detecting chromatographic signals in the separation process; the sample injection valve is used for sample injection;
the liquid chromatographic separation column array is formed by connecting a plurality of chromatographic separation columns in parallel through a multi-position selection valve, and only one chromatographic separation column can be conducted at the same time; a fixed inlet and a fixed outlet are arranged outwards, and at least one bypass is arranged, and the bypass is connected with the separation column in parallel through a multi-position selection valve; when the bypass is conducted, other chromatographic separation columns cannot be conducted, and when other chromatographic separation columns are conducted, the bypass cannot be conducted; the number of chromatographic separation columns is determined according to the requirement;
the enrichment column array A and the enrichment column array B are formed by connecting a plurality of chromatographic enrichment columns in parallel through a multi-position selection valve, and only one enrichment column can be conducted at the same time; at least one bypass is connected with the enrichment column in parallel through a multi-position selection valve; when the bypass is conducted, other enrichment columns cannot be conducted, and when other enrichment columns are conducted, the bypass cannot be conducted; the number of the enrichment columns is determined according to the requirement; two interfaces are externally arranged and respectively defined as an interface X and an interface Y;
the high-efficiency liquid chromatography gradient pump A and the high-efficiency liquid chromatography gradient pump B are connected with an inlet of a gradient mixer A, an outlet of the gradient mixer A is connected with an inlet of a sample injection valve, an outlet of the sample injection valve is connected with a first position of a two-position ten-way valve, a third position of the two-position ten-way valve is connected with an interface X of an enrichment column array B, an interface Y of the enrichment column array B is connected with a third position of the two-position ten-way valve, a second position of the two-position ten-way valve is connected with a seventh position, the sixth position of the two-position ten-way valve is connected with an inlet of a liquid chromatography separation column array, an outlet of the liquid chromatography separation column array is connected with a detector, an outlet of the detector is connected with an inlet of the gradient mixer B, a diluent pump is connected with an inlet of the gradient mixer B, an outlet of the gradient mixer B is connected with a second position of the two-position ten-way valve, and the eighth position of the two, the interface Y of the enrichment column array A is connected with the fifth position of the two-position ten-way valve, and the fourth position of the two-position ten-way valve is connected with the inlet of the fraction collector;
by controlling the switching state of the two-position ten-way valve, the system is switched from the upper one-dimensional separation state to the lower one-dimensional separation state, the circulating chromatographic function is completed, and the chromatographic separation function of multi-dimensional full-on-line detection is realized.
2. The multi-dimensional liquid chromatography separation system of claim 1, wherein the sample injection valve is connected in a bypass of the enrichment column array A or the enrichment column array B, and the outlet of the gradient mixer A is connected with the No. i position of the two-position ten-way valve.
3. The multi-dimensional liquid chromatography separation system of claim 1 or 2, wherein a plurality of said enrichment column arrays are connected in series to form a multi-stage enrichment column array, and the operation control is consistent with that of a single-stage enrichment column array, and only one enrichment column can be conducted at the same time; when the multi-stage enrichment column array is in a bypass conduction state, the enrichment column array of each stage is in bypass conduction.
4. A multi-dimensional liquid chromatography separation system according to claim 1 or 2, wherein the two-position ten-way valve is one valve or consists of a plurality of valves, operating on the two-position ten-way valve switching valve principle.
5. A multi-dimensional liquid chromatography separation system according to claim 3, wherein the two-position ten-way valve is one valve or consists of a plurality of valves, operating on the two-position ten-way valve switching valve principle.
6. A multi-dimensional liquid chromatography separation system according to claim 1, 2 or 5, wherein said HPLC gradient pump A and said HPLC gradient pump B are each composed of two unit pumps, or composed of one multi-unit gradient pump; the diluent pump is a unit pump or a multi-element pump.
7. A multi-dimensional liquid chromatography separation system according to claim 3, wherein said hplc gradient pump a and said hplc gradient pump B are each comprised of two unit pumps, or are comprised of one multi-unit gradient pump; the diluent pump is a unit pump or a multi-element pump.
8. A multi-dimensional liquid chromatography separation system according to claim 4, wherein said HPLC gradient pump A and said HPLC gradient pump B are each composed of two unit pumps, or composed of one multi-unit gradient pump; the diluent pump is a unit pump or a multi-element pump.
9. A multi-dimensional liquid chromatography separation system according to claim 1 or 2 or 5 or 7 or 8, wherein said detector is any device for detecting chromatographic signals during separation, including but not limited to UV detector, diode array detector, evaporative light scattering detector or mass spectrometry detector, including a combined detection system of multiple detectors.
10. A multi-dimensional liquid chromatography separation system according to claim 6 wherein said detector is any device for detecting chromatographic signals during separation including but not limited to UV detectors, diode array detectors, evaporative light scattering detectors or mass spectrometry detectors, including multiple detectors combined detection systems.
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| CN113607857A (en) * | 2021-01-17 | 2021-11-05 | 李宜珊 | Analytical circulating multidimensional liquid chromatography separation system |
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| CN113607857A (en) * | 2021-01-17 | 2021-11-05 | 李宜珊 | Analytical circulating multidimensional liquid chromatography separation system |
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