CN207586196U - The multidimensional liquid chromatographic separation system of full on-line checking based on same detector - Google Patents
The multidimensional liquid chromatographic separation system of full on-line checking based on same detector Download PDFInfo
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Abstract
The utility model provides a kind of multidimensional liquid chromatographic separation system of the full on-line checking based on same detector, including high performance liquid chromatography gradient pump A, high performance liquid chromatography gradient pump B, efficient liquid phase diluting pump, gradient mixer A, gradient mixer B, sampling valve, enriching column array A, enriching column array B, fraction collector, liquid chromatography separation column array, detector, two ten-way valves and connecting line；The conversion of upper one-dimensional discrete state one-dimensional discrete state under is realized by two ten-way valve switchings, realizes three-dimensional or three-dimensional Yi Shang chromatographic isolation.Each dimension chromatography column is selected by liquid chromatography separation column array, based on same gradient elution elution system and same detector, full on-line monitoring and the control of multi-dimensional chromatograph separation is realized, realizes that enriching column and splitter clean-up performance are controllable.Each dimension separation is connected, and be enriched with or trapped using diluting pump auxiliary compounds by enriching column.The utility model is combined by selecting different chromatographic stationary phases and flowing, realizes efficiently separating for the complex system sample high to separating difficulty.
Description
Technical field
The utility model belongs to high performance liquid chromatography separation technical field, is related to a kind of complete online based on same detector
The Multidimensional HPLC piece-rate system of detection.
Background technology
With the development of isolation technics, seek and detach the ingredient in complex sample system oneself becomes hot research field.
The separating degree that Multidimensional Liquid Chromatography Technology is effectively improved complex sample ingredient and detaches by improving peak capacity, becomes flash chromatography point
From technological development direction.Multidimensional Liquid Chromatography Technology is to be implanted sequentially the eluent of the first dimension chromatographic column of sample subsequently to tie up
The liquid chromatogram joint technology that chromatographic column is further detached.This isolation technics can utilize two or more different
The chromatographic column of separating mechanism carries out the orthogonal separation of sample.Most common multidimensional liquid chromatogram interfacing has 3 kinds：Based on sample
The interfacing of ring；Interfacing based on enriching column (also referred to as trapping column)；Interfacing based on dwell pattern.
Due to technology restriction, two dimension common at present or multidimensional liquid chromatographic system utilize multiple gradient elution systems mostly
System using dwell pattern or continuous mode, can only detect and control last one-dimensional separation process, be not carried out each dimension separation
Overall process monitors and automation transparent control, it is also difficult to monitor the clean-up performance of all enriching columns or splitter, it is difficult to quick weight
Multiple and satisfaction prepares sample needs；It is all independent using one per one-dimensional piece-rate system in certain multi-dimensional chromatograph piece-rate systems
Detector and independent gradient elution system, entire chromatographic fractionation system cost are higher.
Utility model content
The purpose of this utility model is to detach some problems in the prior art for above-mentioned multidimensional liquid chromatogram, is carried
For a kind of low-cost separation process can online detection and control entirely Multidimensional HPLC device.By monitoring on-line and controlling entirely
Technology processed realizes that enriching column and splitter clean-up performance are controllable, and then realizes prepared by repeated isolation.Can use dwell pattern or
Faction cut technology by fraction similar in complex sample pre-separation into several properties, is detached with reference to follow-up multidimensional liquid chromatogram, real
The efficient repeated isolation of existing complex sample component.In addition enrichment effect of the compound on enriching column is improved by introducing dilution
Rate.
In order to achieve the above object, the technical solution of the utility model is：
The multidimensional liquid chromatographic separation system of full on-line checking based on same detector, including high performance liquid chromatography gradient
Pump A1, high performance liquid chromatography gradient pump B2, diluting pump 3, gradient mixer A4, gradient mixer B5, sampling valve 6, enriching column
11, two array A7, enriching column array B8, fraction collector 9, liquid chromatography separation column array 10, detector ten-way valves 12 with
And connecting line.The 1. position of two ten-way valves, 2. position, 3. position, 4. position, 5. position, 6. position, 7. position, 8. position, 9. position, 10. position
Only represent syntople, it is not necessary to corresponding with the physical markings of two ten-way valves.The detector 11 is used to detect in separation process
Chromatographic signal.
The entrance of the high performance liquid chromatography gradient pump A1 and high performance liquid chromatography gradient pump B2 and gradient mixer A4 connects
It connects, the outlet of gradient mixer A4 is connect with sampling valve 6, and the outlet of sampling valve 6 is connect with a 1. number position for two ten-way valves, two
The 10. number position of ten-way valve is connect with the entrance of enriching column array A7, the outlet of enriching column array A7 and the 7. number position of two ten-way valves
Connection, the 6. number position of two ten-way valves are connect with the entrance of liquid chromatography separation column array 10, liquid chromatography separation column array 10
Outlet connect with detector 11, the outlet of detector is connect with the entrance of gradient mixer B5, and diluting pump 3 is mixed with gradient
The entrance connection of device B5, the outlet of gradient mixer B5 is connect with a 9. number position for two ten-way valves；The 8. number position of two ten-way valves
It is connect with a 3. number position；The 2. number position of two ten-way valves is connect with the entrance of enriching column array B8, the outlet of enriching column array B8 with
The 5. number position connection of two ten-way valves；The 4. number position of two ten-way valves is connect with the entrance of fraction collector 9.
The liquid chromatography separation column array 10 is to be formed in parallel by multiple chromatography columns by multidigit selector valve, same
One moment can only there are one chromatography column be connected；Externally there are one fixed entrance and a fixed outlet, and at least
One bypass, the bypass and splitter pass through the parallel connection of multidigit selector valve；When by-path turn-on, other chromatography columns cannot be led
Logical, being bypassed when other chromatography columns are connected cannot be connected；Depending on the quantity of chromatography column is as needed, if three
Dimension, then be recommended as 3 splitters, if four-dimensional, be then recommended as 4 splitters.
The enriching column array A7, enriching column array B8 by multiple chromatograph enrichment columns by multidigit selector valve it is in parallel and
Into, synchronization can only there are one enriching column be connected；Externally there are one fixed entrance and a fixed outlet, and at least
There are one bypassing, the bypass and enriching column pass through the parallel connection of multidigit selector valve；When by-path turn-on, other enriching columns cannot be connected,
Being bypassed when other enriching columns are connected cannot be connected；Depending on the quantity of enriching column is as needed, it is primarily limited to length of pipe
And installation space.Multiple enriching column arrays can connect, i.e. the outlet of upper level enriching column array and secondary enriching column array
Entrance connects, and forms multistage enriching column array, and operation control is consistent with single-stage enriching column array, and synchronization can only there are one rich
Clustered column is connected；When the multistage enriching column array is by-path turn-on state then every grade of enriching column array all in by-path turn-on.
Based on the pipeline connecting mode of above-mentioned multidimensional liquid chromatographic separation system, by the switching for controlling two ten-way valves 12
State, realization system are converted to down one-dimensional discrete state from upper one-dimensional discrete state, complete cyclical chromatography function, realize that multidimensional is complete
The chromatographic isolation function of on-line checking.
Sampling valve 6 can also be connected to enriching column array A7 or enrichment described in above-mentioned multidimensional liquid chromatographic separation system
In the bypass of column array B8；At this point, the outlet of gradient mixer A4 is connect with a 1. number position for two ten-way valves；Above-mentioned connection variation
The use of system is not influenced, and the dimension of enriching column is only redefined in control.
Two ten-way valves can be a valve, can also be switched by one or more valve groups into and by two ten-way valves
Valve principle is run.The sampling valve is a sampling device, can be two six logical switch sampling valves or injector；It can
Loading valve is switched with the multidigit for being other realization liquid or solid-state loading；It can also be the chromatographic column of a realization solid-state loading.
The high performance liquid chromatography gradient pump A1, high performance liquid chromatography gradient pump B2 be made of two modular pumps or by
One polynary gradient pump composition.The diluting pump 3 is efficient liquid phase diluting pump, is a modular pump or is one polynary
Pump.The high performance liquid chromatography gradient pump A1 and high performance liquid chromatography gradient pump B2 and diluting pump 3, diluent is water, salt
Solution, methanol, acetonitrile, acetone, ethyl alcohol or normal alkane solvent, eluant, eluent for methanol, acetonitrile, ethyl alcohol, water and its mixture,
The common organic solvent such as n-alkane.
The detector, for detecting the device of chromatographic signal in separation process, includes but are not limited to ultraviolet inspection to be various
Device, diode array detector, evaporative light scattering detector or mass detector are surveyed, can be one or more detector connection
It closes.
The splitter array 10, enriching column array A7, enriching column array B8 chromatographic column can select it is identical or different
Filler, the filler be silica gel, reverse phase silica gel matrix fill or various macropores with C18, Xion, C8, CN base or amino
Adsorb the fillers such as resin and ion exchange resin.
Multi-position switching valve is a kind of way of realization of column array；When there are one should during pillar conducting in a column array column
Other pillars and bypass will be not turned in column array, and other pillars are not turned in the column array when the column array bypass is connected.
Compared with prior art, the beneficial effects of the utility model are：
(1) the separation process whole process of the multidimensional liquid chromatographic system constructed by the utility model is measurable and controllable, enriching column and
Splitter clean-up performance can detect.In the case where complex sample or fraction enter before unidimensional system separation, suitable chromatographic column is selected
It is divided into several fractions according to property and detection signal, is then pointedly established down according to the property of each fraction one-dimensional
Separation method can further improve the resolution ratio of method.The utility model multidimentional system interface is utilized using enrichment column technology
Beneficiation technologies collect the fraction obtained by chromatographic isolation, and introduce dilution before compound enters enriching column to improve enrichment effect
Rate.
(2) the multidimensional liquid chromatographic system constructed by the utility model, suitable for the highly difficult repetition of complex sample system
Analysis, separation and preparation.Different chromatography column combinations is selected according to the difference of analyzed sample or fraction, system uses control spirit
It is living, it can be run under various modes, for example, one-dimensional clastotype, two dimensional separation pattern, three-dimensional clastotype etc.；System into
This is low, and autgmentability is strong, has boundless application prospect in the highly difficult separation analysis of complex system sample.
Description of the drawings
The odd numbers dimension separation shape such as Fig. 1 is tieed up for multidimensional liquid chromatographic separation system first provided by the utility model, third dimension
The pipeline jointing construction figure of state；Two ten-way valves are A condition.
The Even-dimensionals such as Fig. 2 is tieed up for multidimensional liquid chromatographic separation system second provided by the utility model, fourth dimension detach shape
The pipeline jointing construction figure of state；Two ten-way valves are B state.
Fig. 3 is the pipeline jointing construction figure of enriching column array A, enriching column array B and liquid chromatography separation column array；
Fig. 4 (a) be two six-way injection valve sample loading condition (LOAD states, A condition) pipeline jointing construction figures, the shape
Sample is loaded into quantitative loop under state, wherein 4. number position is defined as the entrance of sampling valve, 5. number position is defined as going out for sampling valve
Mouthful；
Fig. 4 (b) is two six-way injection valve sample loading condition (INJECT states, B state) pipeline jointing construction figures, should
Sample will be injected into piece-rate system flow path from quantitative loop and detach under state, wherein 4. number position is defined as entering for sampling valve
Mouthful, 5. number position is defined as the outlet of sampling valve；
Fig. 5 is the Multidimensional HPLC piece-rate system structure chart of the utility model embodiment 1；
In figure：1 high performance liquid chromatography gradient pump A, 2 high performance liquid chromatography gradient pump B, 3 diluting pumps, 4 gradient mixers
A, 5 gradient mixer B, 6 sampling valves, 7 enriching column array A, 8 enriching column array B, 9 fraction collectors, 10 liquid chromatography separation columns
Array, 11 detectors, 12 two ten-way valves.
Specific embodiment
Embodiments discussed below is only a kind of description applied to the utility model patent, not to the utility model
Range be defined, under the premise of the spirit of the design of the utility model is not departed from, those of ordinary skill in the art are to this practicality
The various modifications and improvement that New Scheme is made should all fall into the protection domain that the utility model claims book determines.
The multidimensional liquid chromatographic separation system of full on-line checking based on same detector, including high performance liquid chromatography gradient
Pump A1, high performance liquid chromatography gradient pump B2, diluting pump 3, gradient mixer A4, gradient mixer B5, sampling valve 6, enriching column
11, two array A7, enriching column array B8, fraction collector 9, liquid chromatography separation column array 10, detector ten-way valves 12 with
And connecting line.Wherein, diluting pump 3 is efficient liquid phase diluting pump.
The entrance of the high performance liquid chromatography gradient pump A1 and high performance liquid chromatography gradient pump B2 and gradient mixer A4 connects
It connects, the outlet of gradient mixer A4 is connect with sampling valve 6, and the outlet of sampling valve 6 is connect with a 1. number position for two ten-way valves, two
The 10. number position of ten-way valve is connect with the entrance of enriching column array A7, the outlet of enriching column array A7 and the 7. number position of two ten-way valves
Connection, the 6. number position of two ten-way valves are connect with the entrance of liquid chromatography separation column array 10, liquid chromatography separation column array 10
Outlet connect with detector 11, the outlet of detector is connect with the entrance of gradient mixer B5, and diluting pump 3 is mixed with gradient
The entrance connection of device B5, the outlet of gradient mixer B5 is connect with a 9. number position for two ten-way valves；The 8. number position of two ten-way valves
It is connect with a 3. number position；The 2. number position of two ten-way valves is connect with the entrance of enriching column array B8, the outlet of enriching column array B8 with
The 5. number position connection of two ten-way valves；The 4. number position of two ten-way valves is connect with the entrance of fraction collector 9.Two ten-way valves
1. position, 2. position, 3. position, 4. position, 5. position, 6. position, 7. position, 8. position, 9. position, 10. position only represents syntople, it is not necessary to two ten
The physical markings of port valve correspond to.
Two ten-way valves are A condition in Fig. 1, at this point, high performance liquid chromatography gradient pump A1 and high performance liquid chromatography gradient pump
B2 and gradient mixer A4 forms chromatographic isolation gradient elution mobile phase feed system, the outlet of gradient mixer A4 and sampling valve
6 connections, the outlet of sampling valve 6 are connect with a 1. number position for two ten-way valves, and 1. number position is connected simultaneously with a 10. number position for two ten-way valves
It is connect with the entrance of enriching column array A7, the outlet of enriching column array A7 is connect and with a 7. number position for two ten-way valves through two
The 6. number position of ten-way valve is connect with the entrance of liquid chromatography separation column array 10, selects the arbitrary chromatographic column in splitter array 10
It is detached, the outlet of splitter array 10 is connect with detector 11, and detector 11 detects chromatographic signal, the outlet of detector 11
It is connect with the entrance for diluting mixer B5, diluting pump 3 is connect with the entrance of gradient mixer B5, through diluting mixer B5 dilutions
Sample is flowed out after column, outlet is connect with a 9. number position for two ten-way valves；The 8. number position of two ten-way valves is connect with a 3. number position；Two
The 2. number position of position ten-way valve connect with the entrance of enriching column array B8, enriching column array B8 export and two ten-way valves 5. number
Position connection, realizes the enrichment of separation sample；The 4. number position of two ten-way valves is connect with the entrance of fraction collector 9, realizes sample
It collects.
Two ten-way valves are B state in Fig. 2, at this point, high performance liquid chromatography gradient pump A1 and high performance liquid chromatography gradient pump
B2 and gradient mixer A4 forms chromatographic isolation gradient elution mobile phase feed system, the outlet of gradient mixer A4 and sampling valve
6 connections, the outlet of sampling valve 6 are connect with a 1. number position for two ten-way valves, and 1. number position is connected simultaneously with a 2. number position for two ten-way valves
It is connect with the entrance of enriching column array B8, the outlet of enriching column array B8 is connect and with a 5. number position for two ten-way valves through two
The 6. number position of ten-way valve is connect with the entrance of liquid chromatography separation column array 10, selects the arbitrary chromatographic column in splitter array 10
It is detached, the outlet of splitter array 10 is connect with detector 11, and detector 11 detects chromatographic signal, the outlet of detector 11
It is connect with the entrance for diluting mixer B5, diluting pump 3 is connect with the entrance of gradient mixer B5, through diluting mixer B5 dilutions
Sample is flowed out after column, outlet is connect with a 9. number position for two ten-way valves；10. position and enriching column array A7 through two ten-way valves
Entrance connection, the outlet of enriching column array A7 connect with a 7. number position for two ten-way valves, and realization detaches the enrichment of sample；Through two
The 8. number position of position ten-way valve is connect with a 3. number position；The 4. number position of two ten-way valves is connect with the entrance of fraction collector 9, realizes sample
Product are collected.
Embodiment 1：A kind of Multidimensional HPLC piece-rate system structure
Enriching column array A7 has 9 enriching columns in the embodiment, the 1st enriching column that number consecutively is enriching column array A7,
2nd enriching column, etc., the 9th enriching column that the last one number is enriching column array A7；Enriching column array B8 is two-stage enriching column
Array, every grade of enriching column array have 9 enriching columns, i.e. enriching column array B8 is 18 enriching columns, and number consecutively is enriching column battle array
The 1st enriching column of B8, the 2nd enriching column, etc. are arranged, the 18th enriching column that the last one number is enriching column array B8；Liquid phase color
Spectrum splitter array has 5 splitters, and number consecutively is the 1st splitter, the 2nd splitter, etc., last root is the 5th separation
Column；Two ten-way valves in Fig. 1 are A condition, and two ten-way valves in Fig. 2 are B state.
It is controlled below for the four-dimensional separation process of above-mentioned Multidimensional HPLC piece-rate system structure：
Enriching column and splitter are cleaned first；Switch successively in each enriching column and splitter to flow path, observe detector
Signal judges cleaning effect.
First dimension separation process control：Two ten-way valves are A condition, referring to Fig. 1；Enriching column array A7 is in bypass shape
State；Sample is loaded into the quantitative loop on sampling valve 6；Selection the first dimension chromatography column, for example, the 1st splitter, the chromatography point
It is connected manually from column；When sampling valve 6 is switched to INJECT states, start the first dimension separation；Under the assistance of diluting pump 3, root
According to properties of samples and detection signal successively by fraction using being enriched in the 1st to the 9th enriching column of enriching column array B8, it is enriched with
The the 10th to the 18th enriching column of column array B8 uses when giving over to third dimension separation；So repeatedly, until the 1st of enriching column array B8 the
There are enough compounds into the 9th enriching column, be transferred to the second dimension separation process control；If you do not need to two dimensional separation,
Then enriching column array B8 is constantly in bypass condition, and the collection of multiple fractions is directly carried out using fraction collector 9.
Second dimension separation process control：After first dimension separation process control, sampling valve 6 should switch to LOAD shapes
State, two ten-way valves switch to B state, referring to Fig. 2；Selection the second dimension chromatography column, for example, the 2nd splitter, the chromatography point
It is connected manually from column；Select a sample of the enriching column as two dimensional separation in the 1st to the 9th enriching column of enriching column array B8
Capo；When the enriching column is connected, the second dimension separation process starts；Under the assistance of diluting pump 3, according to properties of samples and detection
Fraction is switched in the 1st to the 9th enriching column of enriching column array A7 and is enriched with by signal successively；If you do not need to the third dimension point
From then the 1st to the 9th enriching column of enriching column array A7 can be eluted successively, multiple fractions are directly carried out using fraction collector 9
Collection；So repeatedly, two dimensional separation is completed；
Third dimension separation process controls：After second dimension separation process control, two ten-way valves switch to A condition, ginseng
See Fig. 1；Sampling valve keeps LOAD states；Third dimension chromatography column is selected, for example, the 3rd splitter, the chromatography column is manual
Conducting；The sample column that an enriching column in the 1st to the 9th enriching column of enriching column array A7 is selected to be detached as the third dimension；When
When the enriching column is connected, third dimension separation process starts；If necessary to carry out fourth dimension separation, then under the assistance of diluting pump 3,
Fraction is switched to successively in the 10th to the 18th enriching column of enriching column array B8 according to properties of samples and detection signal and carry out richness
Faction cut is 9 parts by collection；If you do not need to carry out fourth dimension separation, then it can be by the 1st to the 9th enrichment of enriching column array A7
Column elutes separation successively, and the collection of multiple fractions is directly carried out using fraction collector 9；So repeatedly, third dimension separation is completed.
Fourth dimension separation process controls：After third dimension separation process controls, two ten-way valves switch to B state, ginseng
See Fig. 2；Sampling valve keeps LOAD states；Fourth dimension chromatography column is selected, for example, the 4th splitter, the chromatography column is manual
Conducting；The sample column that an enriching column in the 10th to the 18th enriching column of enriching column array B8 is selected to be detached as fourth dimension；
When the enriching column is connected, fourth dimension separation process starts；It, can will be as the enriching column of sample column under gradient eluent effect
In compound elution, the 4th splitter effect under, carry out fourth dimension separation；Multiple fractions are carried out using fraction collector 9
It collects；So repeatedly, fourth dimension separation is completed.
Embodiment 2：The multidimensional liquid chromatographic separation system is used for the separation of two class compounds
There are many application models for the multidimensional liquid chromatographic separation system, in addition to above-mentioned separation adds Enrichment Mode, are also enriched with
Pattern, enrichment plus Enrichment Mode, enrichment plus clastotype, enrichment bonus point from add Enrichment Mode and various modes joint should
With.
It is illustrated by taking the separation and concentration application of two class compounds as an example.When two ten-way valves are A condition, there is following point
From enrichment application model：
Enrichment Mode：When needing to realize some sample repeated isolation, by the multiple sample introduction of sampling valve, sample selects respectively
Property is enriched in the chromatographic column of enriching column array A7, and splitter array 10 and enriching column array B8 at this time are in bypass condition,
Realize the concentration and separation of a kind of compound；The sample number of enrichment is less than or equal to the chromatographic column quantity of enriching column array A7.
Separation plus Enrichment Mode：The bypass that sample passes through enriching column array A7 is mixed, is detached through splitter array 10, is enriched with
In enriching column array B8, the separation and enrichment of mixing sample are realized.
Enrichment plus Enrichment Mode：It is adsorbed by enriching column array A7 and B8 Fillers selection, realizes point of two class compounds
From enrichment；At this point, splitter array 10 is in bypass condition.
Enrichment bonus point is from adding Enrichment Mode：The sample being enriched on enriching column array A7 passes through the separation of splitter array 10,
It is enriched on enriching column array B8.
When two ten-way valves are B state, also there is similar application pattern.
Claims (10)
1. a kind of multidimensional liquid chromatographic separation system of the full on-line checking based on same detector, which is characterized in that described
Multidimensional liquid chromatographic separation system includes high performance liquid chromatography gradient pump A (1), high performance liquid chromatography gradient pump B (2), dilution
Pump (3), gradient mixer A (4), gradient mixer B (5), sampling valve (6), enriching column array A (7), enriching column array B (8),
Fraction collector (9), liquid chromatography separation column array (10), detector (11), two ten-way valves (12) and connecting line；Institute
State two ten-way valves (12) 1. position, 2. position, 3. position, 4. position, 5. position, 6. position, 7. position, 8. position, 9. position, 10. position only represents adjacent
Relationship, it is not necessary to corresponding with the physical markings of two ten-way valves (12)；The detector (11) is for detecting the color in separation process
Spectrum signal；The sampling valve (6) is for sample introduction；
The high performance liquid chromatography gradient pump A (1) and the entrance of high performance liquid chromatography gradient pump B (2) and gradient mixer A (4)
Connection, the outlet of gradient mixer A (4) are connect with the entrance of sampling valve (6), the outlet of sampling valve (6) and two ten-way valves
(12) 1. number position connection, the 10. number position of two ten-way valves (12) is connect with the entrance of enriching column array A (7), enriching column array A
(7) outlet is connect with a 7. number position for two ten-way valves (12), 6. number position and the liquid chromatography separation column of two ten-way valves (12)
The entrance connection of array (10), the outlet of liquid chromatography separation column array (10) are connect with detector (11), detector (11)
Outlet is connect with the entrance of gradient mixer B (5), and diluting pump (3) is connect with the entrance of gradient mixer B (5), gradient mixing
The outlet of device B (5) is connect with a 9. number position for two ten-way valves (12)；The 8. number position of two ten-way valves (12) is connect with a 3. number position；
The 2. number position of two ten-way valves (12) is connect with the entrance of enriching column array B (8), the outlet of enriching column array B (8) with two ten
The 5. number position connection of port valve (12)；The 4. number position of two ten-way valves (12) is connect with the entrance of fraction collector (9)；
The liquid chromatography separation column array (10) is formed in parallel by multiple chromatography columns by multidigit selector valve, in same a period of time
Carve can only there are one chromatography column be connected；To being externally provided with a fixed entrance and a fixed outlet, and at least one
A bypass, the bypass and splitter pass through the parallel connection of multidigit selector valve；When by-path turn-on, other chromatography columns cannot be connected,
Being bypassed when other chromatography columns are connected cannot be connected；The quantity of chromatography column determines as needed；
Enriching column array A (7), the enriching column array B (8) by multiple chromatograph enrichment columns by multidigit selector valve it is in parallel and
Into, synchronization can only there are one enriching column be connected；Externally there are one fixed entrance and a fixed outlet, and at least
There are one bypassing, the bypass and enriching column pass through the parallel connection of multidigit selector valve；When by-path turn-on, other enriching columns cannot be connected,
Being bypassed when other enriching columns are connected cannot be connected；The quantity of enriching column determines as needed；
By controlling the switching state of two ten-way valves (12), realization system is converted to down one-dimensional separation from upper one-dimensional discrete state
State completes cyclical chromatography function, realizes the chromatographic isolation function of the full on-line checking of multidimensional.
2. a kind of multidimensional liquid chromatogram segregative line of full on-line checking based on same detector according to claim 1
System, which is characterized in that sampling valve (6) can also be connected to enriching column array A (7) in the multidimensional liquid chromatographic separation system
Or in the bypass of enriching column array B (8)；At this point, the outlet of gradient mixer A (4) and the 1. number position of two ten-way valves (12)
Connection；Above-mentioned connection variation does not influence the use of system, and the dimension of enriching column need to be only redefined in control.
3. a kind of multidimensional liquid chromatogram separation of full on-line checking based on same detector according to claim 1 or 2
System, which is characterized in that the multiple enriching column array can be with multistage enriching column array in series, and operation control and single-stage are rich
Clustered column array is consistent, and synchronization can only there are one enriching column conductings；When the multistage enriching column array is by-path turn-on state
Then every grade of enriching column array is all in by-path turn-on.
4. a kind of multidimensional liquid chromatogram separation of full on-line checking based on same detector according to claim 1 or 2
System, which is characterized in that two ten-way valves (12) can be a valve, can also by one or more valve groups into and by two
Position ten-way valve (12) switching valve principle operation.
5. a kind of multidimensional liquid chromatogram segregative line of full on-line checking based on same detector according to claim 3
System, which is characterized in that two ten-way valves (12) can be a valve, can also by one or more valve groups into and by two
Ten-way valve (12) switching valve principle is run.
6. a kind of multidimensional liquid chromatogram point of full on-line checking based on same detector according to claims 1 or 2 or 5
From system, which is characterized in that high performance liquid chromatography gradient pump A (1), the high performance liquid chromatography gradient pump B (2) is by two
Modular pump is formed or is made of a polynary gradient pump；The diluting pump (3) is a modular pump or is a polynary pump.
7. a kind of multidimensional liquid chromatogram segregative line of full on-line checking based on same detector according to claim 3
System, which is characterized in that high performance liquid chromatography gradient pump A (1), the high performance liquid chromatography gradient pump B (2) is by two units
Pump group is formed into or by a polynary gradient pump；The diluting pump (3) is a modular pump or is a polynary pump.
8. a kind of multidimensional liquid chromatogram segregative line of full on-line checking based on same detector according to claim 4
System, which is characterized in that high performance liquid chromatography gradient pump A (1), the high performance liquid chromatography gradient pump B (2) is by two units
Pump group is formed into or by a polynary gradient pump；The diluting pump (3) is a modular pump or is a polynary pump.
9. a kind of multidimensional liquid phase of full on-line checking based on same detector according to claims 1 or 2 or 5 or 7 or 8
Chromatographic fractionation system, which is characterized in that the detector is the various devices for being used to detect chromatographic signal in separation process, including
But UV detector, diode array detector, evaporative light scattering detector or mass detector are not limited only to, can be one
Or multiple detector joints.
10. a kind of multidimensional liquid chromatogram segregative line of full on-line checking based on same detector according to claim 6
System, which is characterized in that the detector, for detecting the device of chromatographic signal in separation process, is included but are not limited to be various
UV detector, diode array detector, evaporative light scattering detector or mass detector can be one or more detections
Device is combined.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108037233A (en) * | 2017-12-28 | 2018-05-15 | 大连博迈科技发展有限公司 | The multidimensional liquid chromatographic separation system of full on-line checking based on same detector |
| CN108562678A (en) * | 2018-02-12 | 2018-09-21 | 大连博迈科技发展有限公司 | The three-dimensional liquid chromatographic separation system of full on-line checking based on same detector |
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| CN108037233B (en) * | 2017-12-28 | 2024-05-03 | 大连博迈科技发展有限公司 | Multi-dimensional liquid chromatographic separation system based on full online detection of same detector |
| CN108562678A (en) * | 2018-02-12 | 2018-09-21 | 大连博迈科技发展有限公司 | The three-dimensional liquid chromatographic separation system of full on-line checking based on same detector |
| CN108562678B (en) * | 2018-02-12 | 2023-09-22 | 大连博迈科技发展有限公司 | Three-dimensional liquid chromatographic separation system based on full online detection of same detector |
| CN113533541A (en) * | 2020-04-15 | 2021-10-22 | 中国科学院上海药物研究所 | Nano-liter liquid phase system with automatic cleaning function and control method |
| CN113533541B (en) * | 2020-04-15 | 2022-07-08 | 中国科学院上海药物研究所 | Nano-liter liquid phase system with automatic cleaning function and control method |
| CN114047274A (en) * | 2021-09-22 | 2022-02-15 | 四川轻化工大学 | Separation and extraction system for nuclides in radioactive sample |
| CN114137141A (en) * | 2021-12-30 | 2022-03-04 | 安图实验仪器(郑州)有限公司 | Multi-mode liquid chromatography system |
| CN114705776A (en) * | 2022-03-31 | 2022-07-05 | 苏州汇通色谱分离纯化有限公司 | Multidimensional liquid chromatography device and method for purifying ginkgetin |
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