A kind of washing device for preparing the employing of tissue stem cell extract
Technical field
This utility model is related to a kind of washing device, specifically a kind of eluting dress for preparing the employing of tissue stem cell extract
Put.
Background technology
Analytical chemistry is an important method of current material detection and analysis, and it is one of analytical chemistry important to chromatograph
Content.The maximum feature of chromatography is that separation efficiency is high, and it can separate the extremely similar material of various properties.And it both can be with
For the Analysis and Identification of a small amount of material, can be used for big quantity of material again isolates and purifies preparation.Accordingly, as a kind of important analysis
Separation means and method, it is widely used in scientific research and commercial production.Now, it is defended in oil, chemical industry, medicine
The fields such as life, bioscience, environmental science, agricultural sciences all play highly important effect.
Eluting is an important step in chromatography method, and its type of elution mainly has simple elution, stepwise elution and gradient
Three kinds of modes of eluting.The difference that type of elution is selected directly influences the accuracy of result, at present the eluting master of various chromatographic columns
Completed by manual, due to due to individual variation, the result that same type of elution occurs also has certain error.It is special
It is not more complicated type of elution:As eluting fluid exchange species is more, washing steps are various, and elution time requirement is long, and
And elution rate also has the type of elution of strict demand, due to due to manual operations, may be because single stepping is slipped up
Cause the failure of whole experiment, and be also a kind of very big consumption to the muscle power of people, even if elution process is not made a fault
Also after operating because of different people, also there is very big difference in result.
Utility model content
The purpose of this utility model is to provide a kind of washing device for preparing the employing of tissue stem cell extract, to solve
The problem proposed in above-mentioned background technology.
For achieving the above object, this utility model provides following technical scheme:
A kind of washing device for preparing the employing of tissue stem cell extract, including support, discharge duct, input and output pipe
It is road, three-way solenoid valve, liquid conducting pipes, peristaltic pump, eluent holding vessel, top cover, eluting pipe, bottom, returnable bottle, unidirectional
Valve, gas-filtering device, air pump and control module, the eluting pipe are vertically fixedly mounted on the bracket, the eluting pipe
Upper and lower ends connect the top cover and the bottom respectively, wherein adsorb material after filling chromatography in the eluting pipe filling out
Material, the delivery outlet of the bottom connect the returnable bottle, and the top inlet of the top cover connects the one of the input and output pipeline
End, the other end of the input and output pipeline connects the discharge duct respectively by the three-way solenoid valve and the liquid is defeated
The other end of one end of pipeline, the liquid conducting pipes is sent to connect the eluent holding vessel by the peristaltic pump, it is described
The side of returnable bottle arranges air inlet, and air inlet is sequentially connected the check valve, the gas-filtering device and institute by pipeline
Air pump is stated, it is wherein the gas-filtering device is mainly constituted by plain gauze and activated carbon adsorption layer are compound, the air pump, described
Peristaltic pump and the three-way solenoid valve are all connected with the outfan of the control module.
As further program of the utility model:The end of the eluting pipe and the connection of the top cover and the bottom
Place arranges rubber ring.
As this utility model further scheme:Set between the bottleneck of the delivery outlet of the bottom and the returnable bottle
It is equipped with rubber ring.
Compared with prior art, the beneficial effects of the utility model are:The control air pump and compacted being spaced by control module
Dynamic pump work, coordinates three-way solenoid valve to carry out the control of path, to realize the entrance eluting pipe of eluent interval, while between gas
Entering into for formula of having a rest stir in eluting pipe eluent and filler so that eluting is more abundant, and improves elution efficiency.
Description of the drawings
Fig. 1 is structural representation of the present utility model.
Specific embodiment
Below in conjunction with the accompanying drawing in this utility model embodiment, the technical scheme in this utility model embodiment is carried out
Clearly and completely describe, it is clear that described embodiment is only this utility model a part of embodiment, rather than whole
Embodiment.Based on the embodiment in this utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment for being obtained, belongs to the scope of this utility model protection.
Fig. 1 is referred to, in this utility model embodiment, a kind of washing device for preparing the employing of tissue stem cell extract,
Including support 1, discharge duct 2, input and output pipeline 3, rubber ring 4, three-way solenoid valve 5, liquid conducting pipes 6, peristaltic pump 7,
Eluent holding vessel 8, top cover 9, eluting pipe 10, filler 11, bottom 12, returnable bottle 13, check valve 14, gas-filtering device 15,
Air pump 16 and control module 17, the eluting pipe 10 are vertically fixedly mounted on support 1, and the upper and lower ends of eluting pipe 10 connect respectively
Top cover 9 and bottom 12 are connect, and the end of eluting pipe 10 arranges rubber ring 4 with the junction of top cover 9 and bottom 12, it is close so as to ensure
Adsorb the filler 11 of material, the delivery outlet connection returnable bottle of the bottom 12 in sealing property, wherein eluting pipe 10 after filling chromatography
Rubber ring 4, the top inlet connection of the top cover 9 are also provided between 13, and the bottleneck of the delivery outlet and returnable bottle 13 of bottom 12
One end of input and output pipeline 3, the other end of input and output pipeline 3 connect discharge duct 2 and liquid respectively by three-way solenoid valve 5
One end of body conveyance conduit 6, liquid conducting pipes 6 connect eluent holding vessel 8 by peristaltic pump 7, can be delayed by peristaltic pump 7
It is slow quantitative to convey eluent into eluting pipe 10.
The side of the returnable bottle 13 arranges air inlet, and air inlet is sequentially connected check valve 14, gas filtration by pipeline
Device 15 and air pump 16, wherein gas-filtering device 15 are main to be constituted by plain gauze and activated carbon adsorption layer are compound, so as to protect
Card is input to the cleaning gas in returnable bottle 13, provides the gas of certain air pressure by air pump 16 into returnable bottle 13, so that
Obtain gas to rise in eluting pipe 10, due to the effect of 10 inner stuffing 11 of eluting pipe, gas disperses from 11 gap of filler
Middle spilling, drives liquid flowing during gas flows, improves the permeability of liquid, so that eluent can be abundant
By the material eluting on filler 11.
The air pump 16, peristaltic pump 7 and three-way solenoid valve 5 are all connected with the outfan of control module 17, between control module 17
Every control air pump 16 and peristaltic pump 7 work, coordinate three-way solenoid valve 5 to carry out the control of path, to realize eluent interval
Into eluting pipe 10, while gas is step to enter into agitation eluent and filler 11 in eluting pipe 10 so that eluting is more
Fully, and improve elution efficiency.
It is obvious to a person skilled in the art that this utility model is not limited to the details of above-mentioned one exemplary embodiment, and
And in the case of without departing substantially from spirit or essential attributes of the present utility model, can realize that this practicality is new in other specific forms
Type.Therefore, no matter from the point of view of which point, embodiment all should be regarded as exemplary, and is nonrestrictive, this practicality is new
The scope of type is by claims rather than described above is limited, it is intended that the equivalency fallen in claim is contained
All changes in justice and scope are included in this utility model.Any reference in claim should not be considered as restriction
Involved claim.
Moreover, it will be appreciated that although this specification is been described by according to embodiment, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of description is only that those skilled in the art should for clarity
Using description as an entirety, the technical scheme in each embodiment can also Jing it is appropriately combined, form those skilled in the art
Understandable other embodiment.