CN201346409Y - Controllable pressurizing chromatographic separation column - Google Patents

Abstract

The utility model provides a controllable pressurized chromatographic separation column, which is characterized in that it comprises a chromatographic column 1 , a sampler 2 and a pressurizer 3 . The chromatographic column 1 has a scale, and its top is connected with a pressurizer 3, and the sampler 2 and the chromatographic column 1 are connected by two branch pipes with pistons. The utility model provides a controllable pressurized chromatographic separation column, which has the characteristics of increasing the elution flow rate, convenient addition of eluent, and long-term elution and separation. The design of the chromatographic sharing column can control the amount of column filler used, and can also accurately observe the amount of eluent used. Simple to use, easy to operate, suitable for industrial production and laboratory use.

Description

A controllable pressure chromatography separation column

technical field

The utility model relates to the field of separation and purification, in particular to a chromatographic column.

Background technique

In daily laboratory experiments or industrial production, column separation methods are usually used in various fields such as industry, agriculture, medicine, and scientific research. The column separation method is a simple, fast, sensitive and efficient separation and purification method. The separation process of column chromatography is to fill the vertical glass tube with column filler, and elute the separated substance with a certain proportion of chemical mixture. This mixture is called eluent. After a period of elution, it is dispersed by a color band It is several parallel ribbons. The principle is to separate substances with different polarities by changing the polarity of the eluent and elute them in sequence, so as to achieve the effect of separation and purification. However, due to the difference in the density of the column packing and the degree of adsorption, the separation of the sample will be too slow, which will affect the efficiency and effect of the separation. Therefore, in many cases, it is necessary to take some measures, such as pressurization or vacuuming, to enable the separation to be separated quickly. separate from. At present, there are mainly two methods for accelerating the separation of chromatography columns. One is that the bottom of the chromatography column is fixed on the suction filter bottle, and the flow rate of the eluent is accelerated by using a circulating water pump or a vacuum pump, so as to separate quickly. It produces a lot of heat, and the working time should not be too long, which prolongs the time to pass through the column, and its lower end is fixed on the suction filter bottle, which will also affect the reception of the eluted liquid, which is inconvenient to operate; A pressure device is installed on the top of the device, but this device will hinder the operation of adding eluent, which is time-consuming and laborious, and increases the risk factor of instrument damage. There is still no chromatographic separation column device that can be used for a long time, increase the elution flow rate, and facilitate the addition of eluent.

Utility model content

The purpose of the utility model is to overcome the shortcomings of the above-mentioned prior art, and provide a controllable pressurized chromatography separation column, which has the characteristics of increasing the elution flow rate, convenient addition of eluent, and long-term elution and separation.

In order to achieve the above object, the technical scheme adopted by the utility model is as follows:

A controllable pressure chromatographic separation column is composed of a cylindrical chromatographic column and a transparent cylindrical sample injector from bottom to top, and the upper end of the chromatographic column is connected with the pressurizer.

The above-mentioned chromatography column is a cylindrical tube with a piston at the lower end to receive the eluted solution; there is a sand core at the bottom of the chromatography column, which can carry the column packing; the column body has a scale, which can be controlled The loading capacity of the filler can also be observed for the position and speed of the elution of each chromatographic layer; there are two branch pipes with pistons on the upper end of the chromatography column, which are connected to the sampler; there is an interface on the upper end of the column, which is used to connect pressurizer.

The above-mentioned sample injector is a cylindrical transparent container, and the lower end is provided with two branch pipes with pistons, which are connected to the upper end of the chromatography column, which can control the addition of eluent. When the piston is opened, a connected container is formed; There is a scale on the outside of the device, so that the amount of eluent can be observed intuitively, and it is convenient to record and calculate the consumption; there is a cap with a grinding hole on the upper end.

The above-mentioned pressurizer is provided with a pressurization knob, a pressurization timer knob and a pressurization interface, a conduit is connected to the interface, and the other end of the conduit is connected through the upper end interface of the chromatographic column to facilitate pressurization.

Compared with the prior art, the present invention has the following beneficial effects:

The utility model provides a controllable pressurized chromatographic separation column, which has the characteristics of increasing the elution flow rate, convenient addition of eluent, and long-term elution and separation. The design of the chromatographic sharing column can control the amount of column filler used, and can also accurately observe the amount of eluent used. Simple to use, easy to operate, suitable for industrial production and laboratory use.

Description of drawings

Fig. 1 is the structural representation of the utility model.

Detailed ways

Below in conjunction with accompanying drawing, the utility model is described further.

The structure schematic diagram of the present utility model is as shown in accompanying drawing 1, and the controllable pressure chromatography separation column is made up of cylindrical chromatography column 1 and transparent cylindrical sampler 2 from bottom to top, and the upper end of chromatography column 1 is connected with The pressurizer 3 is connected. There is a piston 11 at the lower end of the chromatographic column 1, which is used to control the receiving of the eluted liquid. The inner lower end is provided with a sand core 12, which can carry the column filler. There is a scale 13 on one side of the wall, which is convenient for observing the color of each color layer. Elution speed. Two upper and lower branch pipes 21 and 23 with pistons 22 extend from the upper end of the chromatography column, which are connected to the wall and bottom of the injector 2. When the piston 22 is opened, the chromatography column 1 and the injector 2 form a single Connected container, the sampler 2 is a glass transparent container as a whole, and the container itself is provided with a scale 24, so that the amount of eluent can be observed intuitively, and it is convenient to record and calculate the consumption. cover 25. The upper end interface 14 of the chromatography column 1 is connected with the conduit 34 of the pressurizer 3, the pressurizer 3 is provided with a pressurization knob 31, a pressurization timer knob 32 and a pressurization interface 33, and the conduit 34 is connected with the interface 33, and the conduit 34 is connected with the chromatography column 1 through the interface 14 at the upper end of the chromatography column 1, which is convenient for pressurization.

Operation method: When in use, first install the chromatography column 1 with column packing and load the sample, connect the pressurizer 3 according to Figure 1, and keep all the pistons in the closed state, and put the configured The eluent, open the piston 22 and the cover 25, slowly add the eluent, when the eluent reaches the height of the column packing, cover the cover 25, open the piston 11, and then open the pressure knob 31 of the pressurizer 3, Start to pressurize the elution slowly and carry out the elution. According to the elution situation, the pressurized elution time can be set by controlling the pressurization timer knob 32. When the liquid level of the eluent in the chromatography column 1 is lower than the branch pipe 23 When the pressure is high, the eluent in the injector 2 can be pressed into the chromatographic column through the branch pipe 23 through the branch pipe 21. There is no need to artificially add the eluent during elution until the chromatographic layer is eluted, and the container is received for further use. analysis and research.

Claims (4)

1, a kind of controlled pressurization chromatography splitter is characterized in that being made up of column type chromatographic column (1) and transparent column type sample injector (2) from bottom to top, and the upper end of chromatographic column (1) is connected with pressurizer (3).

2, controlled pressurization chromatography splitter according to claim 1, it is characterized in that described chromatographic column (1) is the column type pipe, there is a piston (11) lower end, the bottom is provided with Sha Xin (12), shaft is provided with scale (13), the shaft upper end is provided with two arms (21), (23), is connected with sample injector (2), and interface on the pillar (14) connects pressurizer (3).

3, controlled pressurization chromatography splitter according to claim 1, it is characterized in that described sample injector (2) is the column type transparent vessel, the lower end is provided with two arm (21), (23) that have piston (22), and be connected with chromatographic column (1) upper end, outside of containers is provided with scale (24), and the upper end is provided with a lid (25).

4, controlled pressurization chromatography splitter according to claim 1, it is characterized in that described pressurizer (3) is provided with pressurization knob (31), pressurization timer knob (32) and pressurization interface (33), interface (33) inserts interface (14) on the chromatographic column by conduit (34).

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