CN101920130A - Sample loading device for column chromatography - Google Patents
Sample loading device for column chromatography Download PDFInfo
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- CN101920130A CN101920130A CN 201010250710 CN201010250710A CN101920130A CN 101920130 A CN101920130 A CN 101920130A CN 201010250710 CN201010250710 CN 201010250710 CN 201010250710 A CN201010250710 A CN 201010250710A CN 101920130 A CN101920130 A CN 101920130A
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- column chromatography
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- injector
- loading device
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Abstract
The invention discloses a sample loading device for common or low-pressure column chromatography, which comprises a sample loader (1), a socketed pipe (5) and a sample cavity; the sample loader (1) is of a cylinder flat shape; one end of the sample loading device is provided with a bell (3), the middle thereof is provided with a cavity (2) and the perimeter thereof is provided with sample discharging holes (4); when a common injector is adopted, the sample cavity is directly connected with the sample loader after a certain amount of filter sample liquid is extracted, or is connected with the sample loader through the socketed pipe (5) and is integrally placed above a column chromatography packed bed, so that the sample liquid rapidly enters from a compressor into the sample loader through the socketed pipe and is sprayed out in a mode of liquid frost or flows out in a mode of water-drop from the holes around, and rapidly and evenly flows into a chromatographic column from the pipe wall of the column chromatography packed bed to finish the sample loading operation. The device of the invention has low manufacturing cost, rapid sample loading speed, evenly sample spreading and convenient operation, and can be widely applied to the chromatographic analysis for a laboratory, industry production and the like.
Description
Technical field
The present invention relates to the sampling device of a kind of common column chromatography or low pressure column chromatography.
Background technology
Along with the increasingly extensive application of liquid chromatogram isolation technics, the last sample technology of high performance liquid chromatograph is satisfactorily resolved because of the use of six-way valve.And the last sample technology of common column chromatography or low pressure column chromatography is perplexing vast chromatogram worker always.The success or failure that the technical relation of last sample operation is separated to sample component.Veteran chromatogram worker dropper commonly used drips sample fast, equably around the chromatography inside pipe wall, can not guarantee fully that serious uneven diffusion does not take place the sample liquid that enters the column packing bed.And the person of lacking experience or beginner's last sample problem is especially outstanding; when the eluent liquid level arrives the filler bed surface; last sample regular meeting often causes the serious inhomogeneous diffusion of sample liquid in the column packing; thereby it is seriously overlapping to cause in the sample each component peaks to occur; inferior separating effect has a strong impact on the result of sample analysis and the efficient of a large amount of preparations.
Summary of the invention
The objective of the invention is to solve the problem that existing common column chromatography or low-pressure column chromatography are manual to be existed when going up sample, provide that a kind of to go up sample speed fast, sample diffusion is even, and is easy to operate, the sample loading device for column chromatography that preparation cost is cheap.
The technical scheme that realizes the object of the invention is: a kind of sample loading device for column chromatography is provided, and it comprises injector, socket pipe and sample cavity; Described injector is the cylinder flat, and an end is a bell, and the centre is a cavity, and the periphery of cavity has sample outlet hole; Described socket pipe one end is connected with the bell of injector, and the other end is connected with sample cavity.
The diameter of described sample outlet hole is 0.1～1.0mm.
The cylinder outside dimension of described injector is less than column chromatography bore 0.5～5mm.
Described socket pipe is flexible pipe or hard tube.
Described sample cavity is an ordinary syringe.
Sample loading device for column chromatography provided by the invention, injector can directly with after ordinary syringe is connected be gone up sample, also can be that injector is earlier gone up sample with being connected with sample cavity (ordinary syringe) after socket pipe is connected again.When sample cavity extracts a certain amount of filtered sample liquid, just be connected with injector, or be connected with injector by socket pipe, place chromatographic column filler bed surface top then, sample liquid directly enters injector fast by sample cavity, or enters injector fast by socket pipe, from spraying the liquid mist or flow out water droplet the aperture all around, sample liquid just flows into chromatographic column quickly and evenly from post bed surface inner tubal wall, and the application of sample operation is just finished.Behind the end of the sample, when the sample liquid level drops in the post filler bed surface, this injector also can be used as the adder of leacheate equally, adds eluent by above identical method, just can or drip leacheate by siphonage with constant flow pump at last and continue chromatographic isolation.
Sample loading device for column chromatography provided by the present invention, can be the size of chromatography pipes commonly used such as φ 10mm, φ 25mm, φ 50mm, φ 100mm and φ 200mm according to internal diameter, the injector outside dimension is made into plurality of specifications such as φ 8mm, φ 23mm, φ 47mm, φ 96mm and φ 195mm respectively; Injector, socket pipe and sample cavity can once be made with the lucite of transparent common plastics, organic solvent-resistant or the materials such as polytetrafluoroethylene (PTFE) of antiacid alkali, and socket pipe can also adopt band tubing.This device both can be processed into disposable aseptic sample loading device for column chromatography, also can make repeatedly the durable type sample loading device for column chromatography that uses, can be widely used in fields such as the chromatography laboratory of various occasions such as school, research institute, enterprise and suitability for industrialized production.
Description of drawings
Fig. 1 is the injector of a kind of sample loading device for column chromatography of providing of the embodiment of the invention and the structural representation of socket pipe.
Among the figure: 1, injector; 2, cavity; 3, sample outlet hole; 4, bell; 5, socket pipe.
The specific embodiment
Below in conjunction with drawings and Examples the present invention is further described.
Embodiment one
Present embodiment provides injector for the glass column of internal diameter 25mm carries out the sephadex low-pressure chromatography.
Referring to accompanying drawing 1, a kind of sample loading device for column chromatography that present embodiment provides, it comprises injector 1 and socket pipe 5, sample cavity adopts medical ordinary syringe; One end of injector 1 is a bell 3, and the centre is a cavity 2, and the periphery of cavity 2 has sample outlet hole 4; One end of socket pipe 5 is connected with the bell 3 of injector 1, and the injection outlet of the other end and ordinary syringe is connected.
Sample outlet hole can be established 12～16, and its diameter is 0.1～1.0mm.
The cylinder outside dimension of injector can be less than column chromatography bore 0.5～5mm.
The injector material is the polyflon of energy organic solvent-resistant and strong acid and strong base.
The sampling device that present embodiment provides carries out the sephadex low-pressure chromatography when analyzing being used for the 25mm glass column, and other device that is used is:
UV-detector: Bio-Rad detector;
Fluid pipe: polyfluortetraethylene pipe;
Constant flow pump drives: eluent is a methyl alcohol: and carrene (1: 1, V/V).
Sephadex column is after the abundant balance of ethanol/methylene eluent, when eluent arrives the sephadex column bed surface, the outflow fluid valve arrheas, with the quantitative draw samples solution of syringe, closely cooperate by socket pipe and injector then, at last whole device is put into post inner filling material liquid level top, advance injector syringe, sample liquid enters injector inside by socket pipe fast, pour into aperture on every side at last from the bottom, the drop of the atomizing sample liquid of ejection aperture or outflow aperture evenly and apace flows into the filler bed surface along vial wall, stops to promote syringe, takes out whole sampling device and just finishes to go up the sample operation.When the sample liquid level drops in the post when filling bed surface, can add eluent by above-mentioned identical operations with another injector, carry out chromatography with constant flow pump or by siphonage dropping eluent at last.
Claims (5)
1. sample loading device for column chromatography, it is characterized in that: it comprises injector (1), socket pipe (5) and sample cavity; Described injector (1) is the cylinder flat, and an end is bell (3), and the centre is cavity (2), and the periphery of cavity (2) has sample outlet hole (4); Described socket pipe (5) one ends are connected with the bell (3) of injector (1), and the other end is connected with sample cavity.
2. a kind of sample loading device for column chromatography according to claim 1 is characterized in that: the diameter of described sample outlet hole (4) is 0.1～1.0mm.
3. a kind of column chromatography sample applicator according to claim 1 is characterized in that: the cylinder outside dimension of described injector (1) is less than column chromatography bore 0.5～5mm.
4. a kind of column chromatography sample applicator according to claim 1 is characterized in that: described socket pipe (5) is flexible pipe or hard tube.
5. a kind of column chromatography sample applicator according to claim 1 is characterized in that: described sample cavity is an ordinary syringe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010250710 CN101920130B (en) | 2010-08-11 | 2010-08-11 | Sample loading device for column chromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010250710 CN101920130B (en) | 2010-08-11 | 2010-08-11 | Sample loading device for column chromatography |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101920130A true CN101920130A (en) | 2010-12-22 |
| CN101920130B CN101920130B (en) | 2013-01-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010250710 Expired - Fee Related CN101920130B (en) | 2010-08-11 | 2010-08-11 | Sample loading device for column chromatography |
Country Status (1)
| Country | Link |
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| CN (1) | CN101920130B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106807114A (en) * | 2015-11-30 | 2017-06-09 | 中美华世通生物医药科技（武汉）有限公司 | Taper chromatographic fractionation system and its method for isolating and purifying |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020041938A1 (en) * | 2000-08-21 | 2002-04-11 | Showa Denko Kabushiki Kaisha | Packing material and cartridge for solid phase extraction |
| WO2003061806A1 (en) * | 2001-12-21 | 2003-07-31 | Trikonex Ab | Flash chromatographic separation process |
| CN2584265Y (en) * | 2002-11-04 | 2003-11-05 | 武汉大学 | Solid phase micro-extraction device |
| CN101531601A (en) * | 2008-03-10 | 2009-09-16 | 北京德众万全医药科技有限公司 | Method for separating and analyzing dopexamine hydrochloride by using HPLC method |
| CN201346409Y (en) * | 2008-08-15 | 2009-11-18 | 华南师范大学 | Controllable pressurizing chromatographic separation column |
-
2010
- 2010-08-11 CN CN 201010250710 patent/CN101920130B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020041938A1 (en) * | 2000-08-21 | 2002-04-11 | Showa Denko Kabushiki Kaisha | Packing material and cartridge for solid phase extraction |
| WO2003061806A1 (en) * | 2001-12-21 | 2003-07-31 | Trikonex Ab | Flash chromatographic separation process |
| CN2584265Y (en) * | 2002-11-04 | 2003-11-05 | 武汉大学 | Solid phase micro-extraction device |
| CN101531601A (en) * | 2008-03-10 | 2009-09-16 | 北京德众万全医药科技有限公司 | Method for separating and analyzing dopexamine hydrochloride by using HPLC method |
| CN201346409Y (en) * | 2008-08-15 | 2009-11-18 | 华南师范大学 | Controllable pressurizing chromatographic separation column |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106807114A (en) * | 2015-11-30 | 2017-06-09 | 中美华世通生物医药科技（武汉）有限公司 | Taper chromatographic fractionation system and its method for isolating and purifying |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101920130B (en) | 2013-01-02 |
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Address after: Suzhou City, Jiangsu province 215137 Xiangcheng District Ji Road No. 8 Patentee after: Soochow University Address before: 215123 Suzhou City, Suzhou Province Industrial Park, No. love road, No. 199 Patentee before: Soochow University |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130102 Termination date: 20150811 |
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| EXPY | Termination of patent right or utility model |