CN101920130B - Sample loading device for column chromatography - Google Patents
Sample loading device for column chromatography Download PDFInfo
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- CN101920130B CN101920130B CN 201010250710 CN201010250710A CN101920130B CN 101920130 B CN101920130 B CN 101920130B CN 201010250710 CN201010250710 CN 201010250710 CN 201010250710 A CN201010250710 A CN 201010250710A CN 101920130 B CN101920130 B CN 101920130B
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- sample
- column chromatography
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- injector
- loading device
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Abstract
The invention discloses a sample loading device for common or low-pressure column chromatography, which comprises a sample loader (1), a socketed pipe (5) and a sample cavity; the sample loader (1) is of a cylinder flat shape; one end of the sample loading device is provided with a bell (3), the middle thereof is provided with a cavity (2) and the perimeter thereof is provided with sample discharging holes (4); when a common injector is adopted, the sample cavity is directly connected with the sample loader after a certain amount of filter sample liquid is extracted, or is connected with the sample loader through the socketed pipe (5) and is integrally placed above a column chromatography packed bed, so that the sample liquid rapidly enters from a compressor into the sample loader through the socketed pipe and is sprayed out in a mode of liquid frost or flows out in a mode of water-drop from the holes around, and rapidly and evenly flows into a chromatographic column from the pipe wall of the column chromatography packed bed to finish the sample loading operation. The device of the invention has low manufacturing cost, rapid sample loading speed, evenly sample spreading and convenient operation, and can be widely applied to the chromatographic analysis for a laboratory, industry production and the like.
Description
Technical field
The present invention relates to the sampling device of a kind of common column chromatography or low pressure column chromatography.
Background technology
Along with the increasingly extensive application of liquid chromatogram isolation technics, the loading technology of high performance liquid chromatograph is satisfactorily resolved because of the use of six-way valve.And the loading technology of common column chromatography or low pressure column chromatography is perplexing vast chromatogram worker always.The success or failure that the technical relation of loading operation is separated to sample component.Veteran chromatogram worker dropper commonly used drips sample fast, equably around the chromatography inside pipe wall, can not guarantee fully that serious inhomogeneous diffusion does not occur the sample liquid that enters the column packing bed.And the person of lacking experience or beginner's loading problem is especially outstanding; when the eluent liquid level arrives the filler bed surface; usually can cause the serious inhomogeneous diffusion of sample liquid in the column packing during loading; thereby it is seriously overlapping to cause in the sample each component peaks to occur; inferior separating effect has a strong impact on the result of sample analysis and the efficient of a large amount of preparations.
Summary of the invention
The problem that exists when the object of the invention is to solve existing common column chromatography or the manual loading of low-pressure column chromatography provides a kind of loading speed fast, and sample diffusion is even, and is easy to operate, the sample loading device for column chromatography that preparation cost is cheap.
The technical scheme that realizes the object of the invention is: a kind of sample loading device for column chromatography is provided, and it comprises injector, socket pipe and sample cavity; Described injector is the cylinder flat, and an end is bell, and the centre is cavity, and the periphery of cavity has sample outlet hole; Described socket pipe one end is connected with the bell of injector, and the other end is connected with sample cavity.
The diameter of described sample outlet hole is 0.1～1.0mm.
The cylinder outside dimension of described injector is less than column chromatography bore 0.5～5mm.
Described socket pipe is flexible pipe or hard tube.
Described sample cavity is ordinary syringe.
Sample loading device for column chromatography provided by the invention, injector can directly with after ordinary syringe is connected carry out loading, also can be that injector first carries out loading with being connected with sample cavity (ordinary syringe) after socket pipe is connected again.When sample cavity extracts a certain amount of filtered sample liquid, just be connected with injector, or be connected with injector by socket pipe, then place chromatographic column filler bed surface top, sample liquid directly enters injector fast by sample cavity, or enters fast injector by socket pipe, from spraying the liquid mist or flow out water droplet the aperture all around, sample liquid just flows into chromatographic column quickly and evenly from post bed surface inner tubal wall, and the application of sample operation is just finished.Behind the end of the sample, when the sample liquid level drops in the post filler bed surface, this injector also can be used as the adder of leacheate equally, adds eluent by above identical method, just can or drip leacheate by siphonage with constant flow pump at last and continue chromatographic isolation.
Sample loading device for column chromatography provided by the present invention, can be the size of the chromatography pipes commonly used such as φ 10mm, φ 25mm, φ 50mm, φ 100mm and φ 200mm according to internal diameter, the injector outside dimension is made into respectively the plurality of specifications such as φ 8mm, φ 23mm, φ 47mm, φ 96mm and φ 195mm; Injector, socket pipe and sample cavity can once be made with the lucite of transparent common plastics, organic solvent-resistant or the materials such as polytetrafluoroethylene (PTFE) of antiacid alkali, and socket pipe can also adopt band tubing.This device both can be processed into disposable aseptic sample loading device for column chromatography, also can make the durable type sample loading device for column chromatography of Reusability, can be widely used in the fields such as the chromatography laboratory of the various occasions such as school, research institute, enterprise and suitability for industrialized production.
Description of drawings
Fig. 1 is the injector of a kind of sample loading device for column chromatography of providing of the embodiment of the invention and the structural representation of socket pipe.
Among the figure: 1, injector; 2, cavity; 3, sample outlet hole; 4, bell; 5, socket pipe.
The specific embodiment
The invention will be further described below in conjunction with drawings and Examples.
Embodiment one
The present embodiment provides injector for the glass column of internal diameter 25mm carries out the sephadex low-pressure chromatography.
Referring to accompanying drawing 1, a kind of sample loading device for column chromatography that the present embodiment provides, it comprises injector 1 and socket pipe 5, sample cavity adopts medical ordinary syringe; One end of injector 1 is bell 3, and the centre is cavity 2, and the periphery of cavity 2 has sample outlet hole 4; One end of socket pipe 5 is connected with the bell 3 of injector 1, and the injection outlet of the other end and ordinary syringe is connected.
Sample outlet hole can be established 12～16, and its diameter is 0.1～1.0mm.
The cylinder outside dimension of injector can be less than column chromatography bore 0.5～5mm.
The injector material is the polyflon of energy organic solvent-resistant and strong acid and strong base.
The sampling device that the present embodiment provides is when carrying out the analysis of sephadex low-pressure chromatography for the 25mm glass column, other device that is used in conjunction with is:
UV-detector: Bio-Rad detector;
Fluid pipe: polyfluortetraethylene pipe;
Constant flow pump drives: eluent is methyl alcohol: and carrene (1: 1, V/V).
Sephadex column is after the abundant balance of ethanol/methylene eluent, when eluent arrives the sephadex column bed surface, the efflux valve arrheas, with the quantitative draw samples solution of syringe, then closely cooperate by socket pipe and injector, at last whole device is put into post inner filling material liquid level top, advance injector syringe, sample liquid enters injector inside by socket pipe fast, pour at last aperture on every side from the bottom, the drop of the atomizing sample liquid of ejection aperture or outflow aperture evenly and rapidly flows into the filler bed surface along vial wall, stops to promote syringe, takes out whole sampling device and just finishes the loading operation.When the sample liquid level drops in the post when filling bed surface, can add eluent by above-mentioned identical operation with another injector, carry out chromatography with constant flow pump or by siphonage dropping eluent at last.
Claims (3)
1. sample loading device for column chromatography, it is characterized in that: it comprises injector (1), socket pipe (5) and sample cavity; Described injector (1) is the cylinder flat, and an end is bell (3), and the centre is cavity (2), and the periphery of cavity (2) has sample outlet hole (4); Described socket pipe (5) one ends are connected with the bell (3) of injector (1), and the other end is connected with sample cavity; The diameter of described sample outlet hole (4) is 0.1～1.0mm; The cylinder outside dimension of described injector (1) is less than column chromatography bore 0.5～5mm.
2. a kind of sample loading device for column chromatography according to claim 1, it is characterized in that: described socket pipe (5) is flexible pipe or hard tube.
3. a kind of sample loading device for column chromatography according to claim 1, it is characterized in that: described sample cavity is ordinary syringe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010250710 CN101920130B (en) | 2010-08-11 | 2010-08-11 | Sample loading device for column chromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010250710 CN101920130B (en) | 2010-08-11 | 2010-08-11 | Sample loading device for column chromatography |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101920130A CN101920130A (en) | 2010-12-22 |
| CN101920130B true CN101920130B (en) | 2013-01-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010250710 Expired - Fee Related CN101920130B (en) | 2010-08-11 | 2010-08-11 | Sample loading device for column chromatography |
Country Status (1)
| Country | Link |
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| CN (1) | CN101920130B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106807114A (en) * | 2015-11-30 | 2017-06-09 | 中美华世通生物医药科技（武汉）有限公司 | Taper chromatographic fractionation system and its method for isolating and purifying |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6602928B2 (en) * | 2000-08-21 | 2003-08-05 | Showa Denko Kabushiki Kaisha | Packing material and cartridge for solid phase extraction |
| SE0104412D0 (en) * | 2001-12-21 | 2001-12-21 | Trikonex Ab | Flash chromatographic method |
| CN2584265Y (en) * | 2002-11-04 | 2003-11-05 | 武汉大学 | Solid phase micro-extraction device |
| CN101531601B (en) * | 2008-03-10 | 2014-07-09 | 北京德众万全医药科技有限公司 | Method for separating and analyzing dopexamine hydrochloride by using HPLC method |
| CN201346409Y (en) * | 2008-08-15 | 2009-11-18 | 华南师范大学 | Controllable pressurizing chromatographic separation column |
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2010
- 2010-08-11 CN CN 201010250710 patent/CN101920130B/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN101920130A (en) | 2010-12-22 |
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Address after: Suzhou City, Jiangsu province 215137 Xiangcheng District Ji Road No. 8 Patentee after: Soochow University Address before: 215123 Suzhou City, Suzhou Province Industrial Park, No. love road, No. 199 Patentee before: Soochow University |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130102 Termination date: 20150811 |
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| EXPY | Termination of patent right or utility model |