CN220142660U - Gun head small column preparation device based on single particle plug - Google Patents
Gun head small column preparation device based on single particle plug Download PDFInfo
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- CN220142660U CN220142660U CN202321198640.9U CN202321198640U CN220142660U CN 220142660 U CN220142660 U CN 220142660U CN 202321198640 U CN202321198640 U CN 202321198640U CN 220142660 U CN220142660 U CN 220142660U
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- single particle
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- plug
- particle plug
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- 239000002245 particle Substances 0.000 title claims abstract description 59
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 238000011049 filling Methods 0.000 claims abstract description 12
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 230000010412 perfusion Effects 0.000 claims abstract description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 17
- 239000004005 microsphere Substances 0.000 claims description 7
- -1 polypropylene Polymers 0.000 claims description 6
- 239000000377 silicon dioxide Substances 0.000 claims description 4
- 239000004812 Fluorinated ethylene propylene Substances 0.000 claims description 3
- 239000004743 Polypropylene Substances 0.000 claims description 3
- HQQADJVZYDDRJT-UHFFFAOYSA-N ethene;prop-1-ene Chemical group C=C.CC=C HQQADJVZYDDRJT-UHFFFAOYSA-N 0.000 claims description 3
- 229920009441 perflouroethylene propylene Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 3
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 3
- 239000000945 filler Substances 0.000 abstract description 17
- 238000001514 detection method Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 10
- 239000010453 quartz Substances 0.000 description 8
- 235000012239 silicon dioxide Nutrition 0.000 description 8
- 239000000499 gel Substances 0.000 description 7
- 238000011068 loading method Methods 0.000 description 7
- AUHDWARTFSKSAC-HEIFUQTGSA-N (2S,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)-2-(6-oxo-1H-purin-9-yl)oxolane-2-carboxylic acid Chemical compound [C@]1([C@H](O)[C@H](O)[C@@H](CO)O1)(N1C=NC=2C(O)=NC=NC12)C(=O)O AUHDWARTFSKSAC-HEIFUQTGSA-N 0.000 description 5
- GRSZFWQUAKGDAV-UHFFFAOYSA-N Inosinic acid Natural products OC1C(O)C(COP(O)(O)=O)OC1N1C(NC=NC2=O)=C2N=C1 GRSZFWQUAKGDAV-UHFFFAOYSA-N 0.000 description 5
- 229940028843 inosinic acid Drugs 0.000 description 5
- 235000013902 inosinic acid Nutrition 0.000 description 5
- 239000004245 inosinic acid Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 4
- 239000008363 phosphate buffer Substances 0.000 description 3
- 238000011033 desalting Methods 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000000825 ultraviolet detection Methods 0.000 description 2
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010844 nanoflow liquid chromatography Methods 0.000 description 1
- 238000002414 normal-phase solid-phase extraction Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000000859 sublimation Methods 0.000 description 1
- 230000008022 sublimation Effects 0.000 description 1
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- 230000007704 transition Effects 0.000 description 1
Abstract
The utility model discloses a gun head small column preparation device based on a single particle plug, and relates to the technical field of chromatographic microcolumns. The utility model comprises a gun head and a single particle plug, wherein the gun head comprises a filling section and a gun nozzle section, the inner diameter of the filling section is larger than that of the gun nozzle section, the single particle plug is a plug body with a perfusion hole structure and is fixed in the gun nozzle section and is used for embedding the single particle plug into the gun nozzle section, and a column bed is formed by filtering homogenate. According to the utility model, the single particle plug is arranged in the gun head, the positive pressure is applied to the gun head, the solution can be discharged through the hole of the single particle plug, and the filler is reserved in the ball head, so that a column bed is formed, the convenience of gun head preparation of gun head small columns is greatly improved, the prepared gun head small columns based on the single particle plug can achieve a good sample pretreatment effect, and can be combined with a detection end, so that online sample pretreatment is realized.
Description
Technical Field
The utility model belongs to the technical field of chromatographic microcolumns, and particularly relates to a gun head small column preparation device based on a single particle plug.
Background
The pretreatment of the sample refers to the technology of separating, concentrating, desalting and purifying the target in the mixed substrate by means of extraction, filtration, centrifugation, sublimation, chromatography and the like before the target in the sample is inspected, and is widely applied to various fields of chemical industry, food, agricultural products, medicines and biochemical researches. The necessary sample pretreatment profoundly affects the correct use of the analytical instrument, the accuracy, precision and efficiency of the analytical method, and thus the measurement result of the sample.
The sample pretreatment device of the pipette tip type, such as a pipette tip type solid phase extraction device, a pipette tip type gel desalting column and the like, is skillfully simplified. The lance tip cavity is typically used to form a section of functional microcolumn that can perform adsorption or distribution to pre-treat a trace sample. In-situ polymerization of a section of integral bed in the gun head cavity does not need to consider the additional installation of a plunger, but the process for preparing the integral bed in the gun head is complex, and compared with a filling bed, the integral bed has low mechanical strength, limited chemical tolerance and small contact specific surface. The filling type gun head small column needs to consider the fixing mode of the plunger, and in-situ polymerization of the integral plunger or the adoption of the filling with the particle size larger than the diameter of the gun head outlet is a common mode.
Small particle size packing is preferred in view of its ability to enhance the interaction between the packed bed and the sample solution and thereby more effectively enhance pretreatment results. However, the diameter of the tapered neck or outlet of the existing main flow gun head is in the hundred-micrometer level, and if the gun head is not designed by special structure, the filler in the submicron level cannot be reserved, so that the gun head is inconvenient to prepare gun head small columns.
Disclosure of Invention
The utility model aims to provide a gun head small column preparation device based on a single particle plug, which solves the problem that the existing gun head is inconvenient to prepare gun head small columns by using a gun head by arranging the single particle plug in the gun head, discharging a solution through the single particle plug and retaining a filler to form a column bed.
In order to solve the technical problems, the utility model is realized by the following technical scheme:
the utility model relates to a gun head small column preparation device based on a single particle plug, which comprises a gun head and a single particle plug; the gun head comprises a filling section and a gun nozzle section, and the inner diameter of the filling section is larger than that of the gun nozzle section; the single particle plug is a plug body with a perfusion hole structure and is fixed in the gun nozzle section, and is used for enabling the single particle plug to be embedded into the gun nozzle section, and a column bed is formed by filtering homogenate.
As a preferable technical scheme of the utility model, the gun nozzle section is in a conical tube structure, and the inner diameter of the gun nozzle section gradually decreases from the filling section towards the gun nozzle.
As a preferable technical scheme of the utility model, the diameter of the single particle plug is larger than the inner diameter of the gun nozzle section and smaller than the inlet diameter of the upper end of the gun nozzle section.
As a preferred technical scheme of the utility model, the single particle plug is of a porous microsphere structure.
As a preferred technical scheme of the utility model, the single particle plug is a silica porous microsphere.
As a preferable technical scheme of the utility model, the gun head is a polypropylene gun head, a polytetrafluoroethylene gun head or a fluorinated ethylene propylene copolymer gun head.
The utility model has the following beneficial effects:
1. according to the utility model, the single particle plug is arranged in the gun head, the positive pressure is applied to the gun head, the solution can be discharged through the pores of the single particle plug, and the filler is reserved in the ball head, so that a column bed is formed, and the convenience of gun head for preparing gun head small columns is greatly improved.
2. According to the utility model, through the single particle plugs with different sizes, various gun heads can be adapted, the fixing mode is simple and easy to operate, the fixing position is flexible and controllable, the filler is kept complete, the column bed is transparent, and the prepared gun head small column based on the single particle plug can achieve a good sample pretreatment effect and can be combined with a detection end for use, so that online sample pretreatment is realized.
Of course, it is not necessary for any one product to practice the utility model to achieve all of the advantages set forth above at the same time.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic structural view of a gun head small column preparation device based on a single particle plug;
FIG. 2 is a half cross-sectional view of the gun head;
FIG. 3 is a flow chart of the preparation of the gun head pillars in the example;
FIG. 4 is a schematic diagram of a gun head and an elastic quartz capillary tube according to the second embodiment;
FIG. 5 is a chromatogram before and after inosinic acid enrichment in example III;
in the drawings, the list of components represented by the various numbers is as follows:
1-gun head, 2-single particle plug, 3-quartz capillary, 4-injector, 5-filler homogenate, 6-elastic quartz capillary, 101-filler section and 102-gun nozzle section.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In the description of the present utility model, it should be understood that the terms "open," "upper," "lower," "thickness," "top," "middle," "length," "inner," "peripheral," and the like indicate orientation or positional relationships, merely for convenience in describing the present utility model and to simplify the description, and do not indicate or imply that the components or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present utility model.
Example 1
Referring to fig. 1 and 2, the utility model discloses a gun head small column preparation device based on a single particle plug, which comprises a gun head 1 and a single particle plug 2, wherein the gun head 1 comprises a filler section 101 and a gun nozzle section 102, the inner diameter of the filler section 101 is larger than that of the gun nozzle section 102, and the connecting part of the filler section 101 and the gun nozzle section 102 is in transition through a necking structure.
Wherein the gun nozzle section 102 is in a conical tube structure, and the inner diameter of the gun nozzle section is gradually reduced from the filling section 101 towards the gun nozzle direction. The gun head 1 is a polypropylene gun head, a polytetrafluoroethylene gun head or a fluorinated ethylene propylene copolymer gun head, such as the gun head of the existing pipettor, the gel loading gun head or other common gun heads with long suction nozzles.
The single particle plug 2 is a plug body with a perfusion hole structure and is fixed in the gun nozzle section 102. Specifically, the single particle plug 2 has a porous microsphere structure, such as silica porous microsphere. And, the diameter of the single particle plug 2 is larger than the inner diameter of the gun nozzle section 102 and smaller than the diameter of the inlet at the upper end of the gun nozzle section 102, so that the single particle plug 2 is embedded into the gun nozzle section 102, and the uniform slurry is filtered, so that the packing forms a column bed.
As shown in fig. 3, the specific preparation process is as follows: a 200 mul gel loading gun head with an elongated suction nozzle is taken out, the long suction nozzle of the gel loading gun head is the gun nozzle section 102, and the upper section is the filling section 101.
The dimensions of the required plugging position of the nozzle section 102 are predetermined, and a single-particle plug 2 having a diameter slightly larger than this (typically 100-110% of the diameter) is selected to be introduced from the opening in the filler section 101 of the gun head 1.
And selecting a quartz capillary tube 3 with the outer diameter of 360 mu m, cutting the quartz capillary tube to a proper length, and ensuring that two ends are tidy and smooth. Because the inner diameter of the gun nozzle section 102 is gradually reduced, after the single particle plug 2 is slightly pushed to a position where the single particle plug needs to be plugged by the quartz capillary 3, the single particle plug 2 is slightly pushed to the inner diameter shrinkage direction of the gun nozzle, so that the single particle plug 2 is ensured to be clamped and fixed in the gun nozzle section 102.
Then, the stationary phase filler is prepared into a filler homogenate 5, 200 mu L of the filler homogenate 5 is sucked by a liquid-transferring gun and transferred into a filler section 101 in a gel loading gun head of a fixed single particle plug 2, a positive pressure is applied by the injector 4 through an upper port of the filler section 101, a solvent is discharged through a gap of the single particle plug 2 by utilizing a kerbstone effect, and the filler is reserved, so that gun head pillars are formed, and convenience of a preparation process is effectively improved.
The single particle plug 2 can also be directly aligned with the gun mouth of the gun head 1 to be placed in the single particle plug 2 on a solid plane, the single particle plug 2 is clamped into the gun mouth of the gun mouth section 102 by pressing and plugging, and the single particle plug 2 can be positioned to a clamping and motionless position by pushing a fine capillary tube which stretches downwards.
Example two
On-line pretreatment platform construction of gun head small column based on single particle plug 2, and the process for preparing a gun head small column filled with silica gel is as follows:
the gun head 1 is a gel-loading gun head, then an elastic quartz capillary tube 6 with a proper length and an outer diameter of 360 mu m and an inner diameter of 75 mu m is cut, and a window with a length of 1cm for ultraviolet detection is manufactured at the middle position through an ablative coating.
This elastic quartz capillary tube 6 is inserted from the nozzle of the lower opening of the gel loading gun head and pushed to the position of the single particle plug 2, as shown in fig. 4. The upper opening of the gel loading gun head is connected with a syringe, and the syringe is arranged on the injection pump. The detection window of the direct-connected capillary tube is fixed in the clamping groove of the ultraviolet detector, so that an online pretreatment-detection platform can be built.
Example III
On the basis of the first embodiment, the method for preparing a gun head concentration column filled with 5 mu m C18 silicon dioxide microspheres is as follows:
1mg/ml of inosinic acid solution was injected from the opening of the tip 1, the sample was washed with phosphate buffer at pH=6.8 to retain inosinic acid on the column, and the target molecule was eluted with phosphate buffer at pH=6.0.
Inosinic acid samples before and after enrichment were detected by nano-flow liquid chromatography, the chromatographic column was a 5 μmC18 packed column of 100 μmx15cm, the mobile phase was pH=6.6 phosphate buffer salt, the flow rate was 400nL/min, the sample injection amount was 4nL, and the UV detection wavelength was 214nm. As shown in fig. 5, the peak intensity after concentration and enrichment was found to be significantly enhanced by the inosinic acid chromatogram concentrated by the gun head cartridge.
In the description of the present specification, the descriptions of the terms "one embodiment," "example," "specific example," and the like, mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present utility model. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the utility model disclosed above are intended only to assist in the explanation of the utility model. The preferred embodiments are not exhaustive or to limit the utility model to the precise form disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the utility model and the practical application, to thereby enable others skilled in the art to best understand and utilize the utility model. The utility model is limited only by the claims and the full scope and equivalents thereof.
Claims (6)
1. Gun head small column preparation facilities based on single particle stopper, its characterized in that: comprises a gun head (1) and a single particle plug (2); the gun head (1) comprises a filling section (101) and a gun nozzle section (102), and the inner diameter of the filling section (101) is larger than the inner diameter of the gun nozzle section (102);
the single particle plug (2) is a plug body with a perfusion hole structure, is fixed in the gun nozzle section (102) and is used for enabling the single particle plug (2) to be embedded into the gun nozzle section (102), and a column bed is formed by filtering homogenate.
2. A device for preparing a small gun head column based on a single particle plug according to claim 1, wherein the gun nozzle section (102) is in a conical tube structure, and the inner diameter of the gun nozzle section (101) is gradually reduced towards the gun nozzle direction.
3. The single particle plug-based gun head and small column preparation device according to claim 2, wherein the diameter of the single particle plug (2) is larger than the gun nozzle inner diameter of the gun nozzle section (102) and smaller than the inlet diameter of the upper end of the gun nozzle section (102).
4. A single particle plug-based gun head and column preparation device according to claim 1 or 3, characterized in that the single particle plug (2) is of porous microsphere structure.
5. The device for preparing the gun head small column based on the single particle plug according to claim 4, wherein the single particle plug (2) is a silica porous microsphere.
6. The single particle plug-based gun head and column preparation device according to claim 1 or 2, wherein the gun head (1) is a polypropylene gun head, a polytetrafluoroethylene gun head or a fluorinated ethylene propylene copolymer gun head.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321198640.9U CN220142660U (en) | 2023-05-18 | 2023-05-18 | Gun head small column preparation device based on single particle plug |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321198640.9U CN220142660U (en) | 2023-05-18 | 2023-05-18 | Gun head small column preparation device based on single particle plug |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220142660U true CN220142660U (en) | 2023-12-08 |
Family
ID=89016403
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321198640.9U Active CN220142660U (en) | 2023-05-18 | 2023-05-18 | Gun head small column preparation device based on single particle plug |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220142660U (en) |
-
2023
- 2023-05-18 CN CN202321198640.9U patent/CN220142660U/en active Active
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