CN101936969A - Sample applicator - Google Patents
Sample applicator Download PDFInfo
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- CN101936969A CN101936969A CN2010102507192A CN201010250719A CN101936969A CN 101936969 A CN101936969 A CN 101936969A CN 2010102507192 A CN2010102507192 A CN 2010102507192A CN 201010250719 A CN201010250719 A CN 201010250719A CN 101936969 A CN101936969 A CN 101936969A
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- China
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- sample
- outer sleeve
- piston
- column chromatography
- outlet holes
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Abstract
The invention discloses a sample applicator for common column chromatography or low-pressure column chromatography, relating to a liquid chromatogram separation and detection technology. The sample applicator comprises an outer sleeve (1) and a piston (2), wherein the periphery of the sleeve wall of the outer sleeve (1) near to the bottom surface is provided with sample outlet holes (3) which are uniformly distributed, and the diameter of the sample outlet holes (3) is 0.1 to 5mm. After the head of the sample applicator is placed in sample liquid, and samples of a certain volume is extracted from the sample outlet holes at the periphery of the sleeve wall of the outer sleeve near to the bottom surface by pulling up the piston, the sample applicator can be placed above a column chromatography feeding bed surface, and the sample liquid flows through the sample outlet holes to crossly spray sprays or water columns to be in contact with inner walls around a chromatographic column by forward pushing and pressing the piston in the outer sleeve of the sample applicator so that the sample liquid can fast and uniformly flow into the chromatographic column from the tube wall above the column chromatography feeding bed continuously; and the sample feeding operation is completed by stopping pushing and pressing the piston. The invention can be widely applied to the field of chromatographic analysis and preparation in labs, research institutes and enterprises in industrial production.
Description
Technical field
The present invention relates to the injector of a kind of common column chromatography or low pressure column chromatography.
Background technology
Along with the increasingly extensive application of liquid chromatography isolation technics, the last sample technology of high performance liquid chromatograph is satisfactorily resolved because of the use of six-way valve.And the last sample technology of common column chromatography or low pressure column chromatography is perplexing vast chromatogram worker always.The success or failure that the technical relation of last sample operation is separated to sample component.Veteran chromatogram worker dropper commonly used drips sample fast, equably around the chromatography inside pipe wall, can not guarantee fully that serious uneven diffusion does not take place the sample liquid that enters the column packing bed.And the person of lacking experience or beginner's last sample problem is especially outstanding; when the eluent liquid level arrives the filler bed surface; last sample regular meeting often causes the serious inhomogeneous diffusion of sample liquid in the column packing; thereby it is seriously overlapping to cause in the sample each component peaks to occur; inferior separating effect has a strong impact on the result of sample analysis and the efficient of a large amount of preparations.
Summary of the invention
The objective of the invention is to solve the problem that existing common column chromatography or low-pressure column chromatography are manual to be existed when going up sample, provide a kind of sample liquid that makes in the post separating filler, to spread evenly, can effectively improve the sample component separating effect, and injector simple in structure, easy to use.
The objective of the invention is to be achieved through the following technical solutions: a kind of injector is provided, and it comprises outer sleeve and piston, and described piston and outer sleeve inner chamber are slidingly connected; The barrel periphery of the close bottom surface of outer sleeve has sample and portals, and they are uniform shape, and the diameter in hole is 0.1～5mm.
Described outer sleeve surface indicates the liquid level rule of show sample liquid capacity.
The outside dimension of described outer sleeve is less than column chromatography bore 0.5～10mm.
Described piston comprises piston rod and is fixed on the rubber piston head of its front end.
When the injector head is placed sample liquid, the pull-up piston, the sample that outer sleeve has near the barrel periphery of bottom surface portal extract the sample of a certain amount of volume after, just can place chromatographic column filler bed surface top, push the piston in the injector outer sleeve forward, sample liquid is just portalled by sample, laterally inwall meets around ejection spray film or water column and the chromatographic column, sample liquid just continuously above the post bed tube wall flow into chromatographic column quickly and evenly, stop pushing piston, the application of sample operation is just finished.
Behind the end of the sample, when the sample liquid level drops in the post filler bed surface, this injector also can be used as the adder of leacheate equally, adds eluent by above identical method, just can or drip leacheate by syphonic effect with constant flow pump at last and continue chromatographic resolution.
Present technique can just can reach the purpose that repeatedly goes up sample by observing the scale of injector sample liquid level.When the sample liquid level drops in the post when filling bed surface, can add eluent by above-mentioned identical operations with another injector, carry out chromatography with constant flow pump or by syphonic effect dropping eluent at last.
Compared with prior art, injector provided by the invention can effectively improve the sample component separating effect, and simple in structure, cheap, easy to use, helps applying.
Description of drawings
Fig. 1 is the structural representation of a kind of injector of providing of the embodiment of the invention.
Among the figure: 1, outer sleeve; 2, piston; 3, sample outlet hole; 4, liquid level rule; 5, piston rod; 6, rubber piston head.
Embodiment
Below in conjunction with drawings and Examples the present invention is further described.
Embodiment one
Present embodiment provides a kind of internal diameter that uses to carry out the injector that the sephadex low-pressure chromatography is analyzed as the 25mm glass chromatography column.
Referring to accompanying drawing 1, it is the structural representation of a kind of injector of providing of present embodiment, and it comprises outer sleeve 1 and piston 2; At the barrel periphery of outer sleeve 1 near the bottom surface, have 16 samples and portal 3, they are uniform shape, and its surface indicates the liquid level rule 4 of show sample liquid capacity.
Piston 2 comprises piston rod 5 and is fixed on the rubber piston head 6 of its front end.
The size of injector outer sleeve 1 is as follows:
Outside dimension is less than the internal diameter 6mm of employed column chromatography pipe.
The injector material is the polyflon of energy organic solvent-resistant and strong acid and strong base.
In the operation of present embodiment, UV-detector is the Bio-Rad detecting device; Fluid pipe is a polyfluortetraethylene pipe; Constant flow pump drives, and eluent is a methyl alcohol: and methylene chloride (1: 1, V/V).
Using method is as follows:
Sephadex column is after the abundant balance of ethanol/methylene eluent, when eluent arrives the sephadex column bed surface, the effluent valve closing of chromatographic column, with the quantitative draw samples solution of injector, then whole device is put into post inner filling material liquid level top, advanced the injection ram tube, sample liquid is poured into the chromatographic column even tube wall and is flowed into the filler bed surface apace by aperture around the outer sleeve bottom fast, stop to promote injection ram, take out whole sampling device and just finish to go up the sample operation.When the sample liquid level drops in the post when filling bed surface, can add eluent by above-mentioned identical operations with another injector, carry out chromatography with constant flow pump or by syphonic effect dropping eluent at last.
Claims (4)
1. injector, it is characterized in that: it comprises outer sleeve (1) and piston (2), described piston (2) is slidingly connected with outer sleeve (1) inner chamber; The barrel periphery of the close bottom surface of outer sleeve (1) has sample and portals (3), and they are uniform shape, and the diameter in hole is 0.1～5mm.
2. a kind of column chromatography sample applicator according to claim 1 is characterized in that: described outer sleeve (1) surface indicates the liquid level rule (4) of show sample liquid capacity.
3. a kind of injector according to claim 1 is characterized in that: the outside dimension of described outer sleeve (1) is less than column chromatography bore 0.5～10mm.
4. a kind of injector according to claim 1 is characterized in that: described piston (2) comprises piston rod (5) and is fixed on the rubber piston head (6) of its front end.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102507192A CN101936969A (en) | 2010-08-11 | 2010-08-11 | Sample applicator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102507192A CN101936969A (en) | 2010-08-11 | 2010-08-11 | Sample applicator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101936969A true CN101936969A (en) | 2011-01-05 |
Family
ID=43390408
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102507192A Pending CN101936969A (en) | 2010-08-11 | 2010-08-11 | Sample applicator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101936969A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106807114A (en) * | 2015-11-30 | 2017-06-09 | 中美华世通生物医药科技（武汉）有限公司 | Taper chromatographic fractionation system and its method for isolating and purifying |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2399718Y (en) * | 1999-12-07 | 2000-10-04 | 王春燕 | Semi-automatic suction tubes |
| CN2401011Y (en) * | 1999-11-25 | 2000-10-18 | 沈国臣 | Liquid fertilizer injection implement |
| CN2560925Y (en) * | 2002-08-06 | 2003-07-16 | 季克和 | Titration liquid-transfer measuring instrument |
| CN201182813Y (en) * | 2008-04-07 | 2009-01-21 | 魏陶军 | Medical respiration auxiliary device |
| CN101500906A (en) * | 2006-07-03 | 2009-08-05 | 密闭系统公司 | Fluid product dispenser |
-
2010
- 2010-08-11 CN CN2010102507192A patent/CN101936969A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2401011Y (en) * | 1999-11-25 | 2000-10-18 | 沈国臣 | Liquid fertilizer injection implement |
| CN2399718Y (en) * | 1999-12-07 | 2000-10-04 | 王春燕 | Semi-automatic suction tubes |
| CN2560925Y (en) * | 2002-08-06 | 2003-07-16 | 季克和 | Titration liquid-transfer measuring instrument |
| CN101500906A (en) * | 2006-07-03 | 2009-08-05 | 密闭系统公司 | Fluid product dispenser |
| CN201182813Y (en) * | 2008-04-07 | 2009-01-21 | 魏陶军 | Medical respiration auxiliary device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106807114A (en) * | 2015-11-30 | 2017-06-09 | 中美华世通生物医药科技（武汉）有限公司 | Taper chromatographic fractionation system and its method for isolating and purifying |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110105 |