CN103920306A - Pressurization device of nanofiber solid phase extraction column - Google Patents
Pressurization device of nanofiber solid phase extraction column Download PDFInfo
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- CN103920306A CN103920306A CN201410148184.6A CN201410148184A CN103920306A CN 103920306 A CN103920306 A CN 103920306A CN 201410148184 A CN201410148184 A CN 201410148184A CN 103920306 A CN103920306 A CN 103920306A
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Abstract
The invention discloses a pressurization device of a nanofiber solid phase extraction column. The pressurization device comprises a tubular shell, a bottom cover, an elastic piece and a pressurization post, wherein a hollow slot parallel to an axis of the shell is formed in the shell; a through hole is formed in the bottom cover; the bottom cover is fixedly connected to the bottom end of the shell; the elastic piece is fixedly connected to the shell; clamping grooves parallel to one other are formed in the outer surface of the pressurization post; the pressurization post is positioned in the shell; the clamping grooves of the pressurization post are matched with the bottom end of the elastic piece. According to the pressurization device, the problem that manual operation possibly wastes time and labor due to extremely high pressure in the process of passing through the nanofiber solid phase extraction column is solved.
Description
Technical field
The present invention relates to a kind of pressue device, relate in particular to a kind of pressue device for nano-fiber solid phase extraction column.
Background technology
In the time that biological sample is carried out to analyzing and testing, sample composition is very complicated, and interfering material is many, and testing concentration is generally all very low, and therefore, but Sample pretreatment is the most complicated, a loaded down with trivial details the most key step all the time.SPE is the one separation method fast and effectively based on liquid chromatogram separation principle.By sample solution, by being pre-charged with the pillar of fixing phase, separated component is retained by forms such as absorption, distribution, ion-exchanges, then uses suitable solvent elution, to reach the object of separation, enrichment and elite.This researcher takes the lead in carrying out analytic sample pre-treatment research with electro spinning nano fiber, the filling solid-phase extraction column taking nanofiber as adsorbent.Because electro spinning nano fiber has high specific area, obviously increase with the interactional site of target molecule, adsorption and desorption speed is fast, compared with existing extraction stuffing, nanofiber solid phase extraction techniques Extraction and enrichment efficiency significantly improves, the consumption of amount of samples and eluant, eluent all greatly reduces, if but contain the stickum such as mucopolysaccharide, protein in sample, will increase column pressure, extended the post time, simple operation is by hand wasted time and energy and also may not reached the effect of pressurization, and this device comes therefrom.
Summary of the invention
technical problem:technical problem to be solved by this invention is: a kind of pressue device for nano-fiber solid phase extraction column is provided, and this pressue device solves nano-fiber solid phase extraction column and crosses in post process, the problem that the manual operations that may occur when pressure is excessive is wasted time and energy.
technical scheme:for solving the problems of the technologies described above, the technical solution used in the present invention is:
A kind of pressue device for nano-fiber solid phase extraction column, it is characterized in that, this pressue device comprises housing, bottom, shell fragment and pressured column in a tubular form, and housing is provided with the dead slot paralleling with the axis of housing, bottom is provided with through hole, and bottom is fixedly connected on the bottom of housing; Shell fragment is fixedly connected on housing; The outer surface of pressured column is provided with the draw-in groove being parallel to each other, and pressured column is arranged in housing, and the draw-in groove of pressured column matches with the bottom of shell fragment.
Further, the described pressue device for nano-fiber solid phase extraction column, also comprises fastener, fastener is arranged in housing, one side of fastener 6 passes the dead slot of housing 1, and the upper end of fastener and the draw-in groove of pressured column match, and matches with the push rod top of solid-phase extraction column in the lower end of fastener.
Further, the internal face of described bottom is provided with internal thread, and the bottom wall of housing is provided with external screw thread, and the internal thread of bottom and the external screw thread of housing match.
Further, middle part and the top of described shell fragment are positioned at hull outside, and the top of shell fragment is positioned at housing top, and the middle part of shell fragment is connected in the outside wall surface of housing by Flexible Connector.
Further, the bottom of shell fragment is through the dead slot of housing, and the bottom of shell fragment tilts to the axis of housing.
Further, the diameter on the top of described pressured column is greater than the diameter of housing inner chamber.
Further, the draw-in groove of described pressured column and the axis of pressured column are perpendicular, and distance between adjacent two draw-in grooves is 3 to 7 millimeters.
Further, the top of described shell fragment is provided with suppending hole.
Further, described Flexible Connector comprises screw and spring, and spring housing is contained on the root of screw, and the bottom of screw is embedded in housing.
Further, the outer surface of described housing is provided with the fin being parallel to each other.
beneficial effect:compared with prior art, technical scheme of the present invention has following beneficial effect:
1. cross post speed controlled.Cross post speed very large on the extraction efficiency impact of SPE, while crossing post speed, object is difficult for absorption, when slower, lose time and requires great effort.Pressue device of the present invention arranges the draw-in groove of some on pressured column surface, in the time that pressured column presses down, a certain draw-in groove of pressured column blocks fixing shell fragment, plays pressured column positioning action, and then controls the pressure in pillar, thereby controls post speed.When shell fragment matches with the draw-in groove of pressured column diverse location, pressured column varies in size to solid-phase extraction column applied pressure, thereby controls the size of post speed.
2., when pressure is excessive, can be easy to exert pressure.During for thickness sample, such as saliva sample, the saliva sample of 0.5mL is crossed post process often needs 10 minutes-30 minutes.Prior art normally uses hand solid-phase extraction column, until sample is excessively complete, wastes time and energy very much.Pressue device of the present invention stops the mode of pressured column resilience by shell fragment, do not need people for exerting pressure in the process of waiting for post, utilizes the suppending hole on shell fragment to be suspended on wait on one side.
3. convenient manual operation, without power is provided, is applicable to various occasions and uses.Prior art as the patent No. be the array solid-phase extraction device based on nanofiber absorption of CN 200910033559, in the time carrying out the sample detection of negligible amounts (1 or 2), by this array inconvenience.Pressue device convenient manual operation of the present invention, without power is provided, is applicable to various occasions and uses.The use of existing solid-phase extraction column all needs the devices such as aspiration pump conventionally, to aspirate or pressuring method operation, needs extra power to supply with power.Pressue device structure of the present invention is easier, more convenient operation.
Brief description of the drawings
Fig. 1 is structural representation of the present invention.
Fig. 2 is the structural representation of pressured column in the present invention.
In figure, have: housing 1, bottom 2, shell fragment 3, pressured column 4, suppending hole 5, fastener 6, draw-in groove 7.
Detailed description of the invention
Below in conjunction with accompanying drawing, technical scheme of the present invention is described in detail.
As shown in Figure 1 to Figure 2, a kind of pressue device for nano-fiber solid phase extraction column of the present invention, comprise housing 1, bottom 2, shell fragment 3 and pressured column 4 in a tubular form, housing 1 is provided with the dead slot paralleling with the axis of housing 1, bottom 2 is provided with through hole, and bottom 2 is fixedly connected on the bottom of housing 1; Shell fragment 3 is fixedly connected on housing 1; The outer surface of pressured column 4 is provided with the draw-in groove 7 being parallel to each other, and pressured column 4 is arranged in housing 1, and the draw-in groove 7 of pressured column 4 matches with the bottom of shell fragment 3.
While using the pressue device for nano-fiber solid phase extraction column of said structure, solid-phase extraction column is positioned at bottom 2 tops, and bottom 2 is for bottling up solid-phase extraction column.Solid-phase extraction column, by the dead slot on housing 1 sidewall, enters in housing 1.Bottom 2 is connected to the bottom of housing 1.Shell fragment 3 is fixedly connected on housing 1.Pressured column 4 moves down from the top end opening of housing 1.Match with the draw-in groove 7 on pressured column 4 in the bottom of shell fragment 3.In the time of dismounting solid-phase extraction column, bottom 2 is unloaded from housing 1, then solid-phase extraction column is taken out in the dead slot from housing 1 sidewall.Pressue device of the present invention is especially applicable to being used in conjunction with nano-fiber solid phase extraction column, because nano-fiber solid phase extraction column is especially applicable to the processing of biological sample, amount of samples is few, is generally several milliliters, generally add just processing sample completely of sample, can be easily that sample is excessively complete.
Further, the described pressue device for nano-fiber solid phase extraction column, also comprises fastener 6, fastener 6 is arranged in housing 1, one side of fastener 6 passes the dead slot of housing 1, and the upper end of fastener 6 matches with the draw-in groove 7 of pressured column 4, matches with the push rod top of solid-phase extraction column in the lower end of fastener 6.By fastener 6 is set, pressured column 4 and the push rod of solid-phase extraction column are coupled together.In the time that pressured column 4 is pulled back, the push rod of solid-phase extraction column is also pulled back with pressured column 4.One side of fastener 6 passes the dead slot of housing 1, is convenient to the installation and removal of fastener 6.
Further, the internal face of described bottom 2 is provided with internal thread, and the bottom wall of housing 1 is provided with external screw thread, and the external screw thread of the internal thread of bottom 2 and housing 1 matches.Bottom 2 is fixedly connected with mode with housing 1 have multiplely, for example, adopt clamping.This patent is preferably threaded.By internal thread and external screw thread are set, make the installation and removal between bottom 2 and housing 1 very convenient, and the fixing that both connect is high.
Further, the middle part of described shell fragment 3 and top are positioned at housing 1 outside, and the top of shell fragment 3 is positioned at housing 1 top, and the middle part of shell fragment 3 is connected in the outside wall surface of housing 1 by Flexible Connector.Shell fragment 3 is arranged on housing 1 outside, is convenient to the installation of shell fragment 3.By Flexible Connector is set, in the time pushing shrapnel 3 top, the bottom of shell fragment 3 can perk, and the upper and lower direction of motion of shell fragment 3 is contrary.Push shrapnel 3 top, can make the bottom of shell fragment 3 depart from the draw-in groove 7 of pressured column 4, the pressured column 4 of now can pulling back.Flexible Connector can adopt various structures, the preferred structure below of the present invention: Flexible Connector comprises screw and spring, and spring housing is contained on the root of screw, and the bottom of screw is embedded in housing 1.
Further, the bottom of described shell fragment 3 is through the dead slot of housing 1, and the bottom of shell fragment 3 tilts to the axis of housing 1.Shell fragment 3 bottoms are set and tilt to the axis of housing 1, make shell fragment 3 bottoms can be good at coordinating with the draw-in groove 7 of pressured column 4.
Further, the diameter on the top of described pressured column 4 is greater than the diameter of housing 1 inner chamber.Like this, pressured column 4 can all not enter in housing 1.Meanwhile, the tip size of pressured column 4 is larger, is convenient to the manual application of force of people.
Further, the draw-in groove of described pressured column 47 is perpendicular with the axis of pressured column 4, and distance between adjacent two draw-in grooves 7 is 3 to 7 millimeters.The draw-in groove 7 of fair amount and spacing is set, is conducive to control pressured column 4 to the solid-phase extraction column size of exerting pressure, control each application of force precision.
Further, the top of described shell fragment 3 is provided with suppending hole 5.Suppending hole 5 is for hanging whole pressue device.
Further, the outer surface of described housing 1 is provided with the fin being parallel to each other.Like this, when people holds the fin of housing 1, be difficult for skidding, increase the fixing of holding.
Exemplify a concrete application example below.
Extract the object in saliva: the pressured column of nano-fiber solid phase extraction column 4 is put into from the top end opening of housing 1, pressured column 4 is inserted, add the nano-fiber solid phase extraction column after activation by getting supernatant after centrifugal 1mL saliva, the pusher section of solid-phase extraction column is connected by fastener 6 with forcing pipe 4, through hole on bottom 2 was overlapped to solid-phase extraction column, after the external screw thread of the internal thread of bottom 2 and housing 1 bottom is tightened, press pressured column 4 downwards from pressured column 4 tops, when pressured column 4 is in position time, while producing certain pressure, match with the draw-in groove 7 on pressured column 4 in the bottom that is fixed on the shell fragment 3 of upper end, Handheld Division, can realize and add needed pressure to crossing post liquid.Matching with the draw-in groove 7 on pressured column 4 in the bottom of shell fragment 3, makes in the time there is no External Force Acting, and pressured column 4 can not move along its axis.A draw-in groove 7 on pressured column 4 is blocked in shell fragment 3 bottoms, ensures that pressured column 4 can up not rebound because of the pressure of nano-fiber solid phase extraction column, often blocks a groove and also can, for solid-phase extraction column provides different pressure, regulate sample to cross the speed of post with this.Crossing in post process, can also utilize the suppending hole 5 on shell fragment 3, whole pressue device is hung up, do not need hand-held.After sample has been crossed, back out screw-cap, take off nano-fiber solid phase extraction column, add eluent wash-out for subsequent analysis operation.
Claims (10)
1. the pressue device for nano-fiber solid phase extraction column, it is characterized in that, this pressue device comprises housing (1), bottom (2), shell fragment (3) and pressured column (4) in a tubular form, housing (1) is provided with the dead slot paralleling with the axis of housing (1), bottom (2) is provided with through hole, and bottom (2) is fixedly connected on the bottom of housing (1); Shell fragment (3) is fixedly connected on housing (1); The outer surface of pressured column (4) is provided with the draw-in groove (7) being parallel to each other, and pressured column (4) is arranged in housing (1), and the draw-in groove (7) of pressured column (4) matches with the bottom of shell fragment (3).
2. according to the pressue device for nano-fiber solid phase extraction column claimed in claim 1, it is characterized in that, also comprise fastener (6), fastener (6) is arranged in housing (1), one side of fastener (6) passes the dead slot of housing (1), and matching with the draw-in groove (7) of pressured column (4) in the upper end of fastener (6), matches with the push rod top of solid-phase extraction column in the lower end of fastener (6).
3. according to the pressue device for nano-fiber solid phase extraction column claimed in claim 1, it is characterized in that, the internal face of described bottom (2) is provided with internal thread, and the bottom wall of housing (1) is provided with external screw thread, and the external screw thread of the internal thread of bottom (2) and housing (1) matches.
4. according to the pressue device for nano-fiber solid phase extraction column claimed in claim 1, it is characterized in that, the middle part of described shell fragment (3) and top are positioned at housing (1) outside, and the top of shell fragment (3) is positioned at housing (1) top, and the middle part of shell fragment (3) is connected in the outside wall surface of housing (1) by Flexible Connector.
5. according to the pressue device for nano-fiber solid phase extraction column claimed in claim 4, it is characterized in that, the bottom of described shell fragment (3) is through the dead slot of housing (1), and the bottom of shell fragment (3) tilts to the axis of housing (1).
6. according to the pressue device for nano-fiber solid phase extraction column claimed in claim 1, it is characterized in that, the diameter on the top of described pressured column (4) is greater than the diameter of housing (1) inner chamber.
7. according to the pressue device for nano-fiber solid phase extraction column claimed in claim 1, it is characterized in that, the draw-in groove (7) of described pressured column (4) and the axis of pressured column (4) are perpendicular, and distance between adjacent two draw-in grooves (7) is 3 to 7 millimeters.
8. according to the pressue device for nano-fiber solid phase extraction column claimed in claim 1, it is characterized in that, the top of described shell fragment (3) is provided with suppending hole (5).
9. according to the pressue device for nano-fiber solid phase extraction column claimed in claim 4, it is characterized in that, described Flexible Connector comprises screw and spring, and spring housing is contained on the root of screw, and the bottom of screw is embedded in housing (1).
10. according to the pressue device for nano-fiber solid phase extraction column claimed in claim 1, it is characterized in that, the outer surface of described housing (1) is provided with the fin being parallel to each other.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410148184.6A CN103920306B (en) | 2014-04-15 | 2014-04-15 | A kind of pressue device for nano-fiber solid phase extraction column |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410148184.6A CN103920306B (en) | 2014-04-15 | 2014-04-15 | A kind of pressue device for nano-fiber solid phase extraction column |
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| CN103920306A true CN103920306A (en) | 2014-07-16 |
| CN103920306B CN103920306B (en) | 2015-11-04 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105344131A (en) * | 2015-11-18 | 2016-02-24 | 河北中烟工业有限责任公司 | Manual solid phase extraction device |
| CN111443074A (en) * | 2020-05-11 | 2020-07-24 | 厦门大学 | Automatic on-line pretreatment and Raman detection device and method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101590333A (en) * | 2009-06-23 | 2009-12-02 | 东南大学 | Array solid-phase extraction device based on nanofiber absorption |
| CN103191584A (en) * | 2013-04-26 | 2013-07-10 | 苏州东奇生物科技有限公司 | Portable solid phase extracting device |
| CN203842349U (en) * | 2014-04-15 | 2014-09-24 | 东南大学 | Pressurizing device for nanofiber solid-phase extraction column |
-
2014
- 2014-04-15 CN CN201410148184.6A patent/CN103920306B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101590333A (en) * | 2009-06-23 | 2009-12-02 | 东南大学 | Array solid-phase extraction device based on nanofiber absorption |
| CN103191584A (en) * | 2013-04-26 | 2013-07-10 | 苏州东奇生物科技有限公司 | Portable solid phase extracting device |
| CN203842349U (en) * | 2014-04-15 | 2014-09-24 | 东南大学 | Pressurizing device for nanofiber solid-phase extraction column |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105344131A (en) * | 2015-11-18 | 2016-02-24 | 河北中烟工业有限责任公司 | Manual solid phase extraction device |
| CN111443074A (en) * | 2020-05-11 | 2020-07-24 | 厦门大学 | Automatic on-line pretreatment and Raman detection device and method thereof |
| CN111443074B (en) * | 2020-05-11 | 2021-08-31 | 厦门大学 | Automatic on-line pretreatment and Raman detection device and method thereof |
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| CN103920306B (en) | 2015-11-04 |
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