CN101590333A - Array solid-phase extraction device based on nanofiber absorption - Google Patents
Array solid-phase extraction device based on nanofiber absorption Download PDFInfo
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- CN101590333A CN101590333A CNA2009100335593A CN200910033559A CN101590333A CN 101590333 A CN101590333 A CN 101590333A CN A2009100335593 A CNA2009100335593 A CN A2009100335593A CN 200910033559 A CN200910033559 A CN 200910033559A CN 101590333 A CN101590333 A CN 101590333A
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Abstract
Array solid-phase extraction device based on nanofiber absorption relates to the trace that is applied to medicine bioengineering complex sample, environmental sample, poisonous substance and pollutant, the fast enriching of trace samplings, the extraction equipment of purifying pre-treatment.Totally 5 layers from the bottom to top, the bottom is a base, be placed with a waste liquid tank that can conveniently take out (9) on the base, on the waste liquid tank (9) receiving tube support (8), upwards be a fixing solid phase extraction column support (7) again, the intubate frame of upwards fixing for one deck (6) again is provided with connecting axle (4) at an end of intubate frame (6), is with a pressing plate (5) that can slide up and down with left rotation and right rotation on the connecting axle (4); Syringe (3) is fixed on the intubate frame (6), and the push rod end of syringe (3) is connected with pressing plate (5), and the front end of syringe (3) is provided with extraction pillar (10); Small test tube (11) is positioned on the receiving tube support (8), and the front end of extraction pillar (10) passes the openend that solid phase extraction column support (7) is positioned at small test tube (11).
Description
Technical field
The present invention relates to a kind of nanofiber solid absorbent that utilizes and come enrichment, extraction sample, carry out the extraction equipment of sample pretreatment, the especially a kind of trace of medicine bioengineering complex sample, environmental sample, poisonous substance and pollutant, the fast enriching of trace samplings, extraction equipment of purifying pre-treatment of being applied to.
Background technology
Modern analytical technique develops towards quick, trace, trace, multicomponent, high selectivity direction, and various new shape precision instruments emerge in an endless stream, and sample pretreatment process has also been proposed more and more higher requirement.Yet statistics shows, analysis operation time 60% in addition mostly be to be used in the pre-treatment of sample, evaluated error is also mostly from sample pretreatment process, so in recent years, sample-pretreating method research and relevant apparatus, instrument development have become the research focus.SPE (Solid-Phase Extraction, SPE) be a kind of effective trace or trace materials isolation of purified means, used device and device comprise SPE pillar and SPE extraction equipment, the SPE pillar normally is filled with the cylindricality or the dish of granular adsorption agent, the SPE extraction equipment is generally taked the drawing-in type working method, therefore must be furnished with accessories such as vavuum pump, blow-dry device, inertia source of the gas, high volume sampler and surge flask except that the main part of ability negative pressure, the auxiliary of instrument configuration is more, price is expensive.A kind of solid phase extraction techniques (Packed-Fiber Solid-PhaseExtraction that fills nanofiber appears recently, PFSPE, in the patent No. is in 200510123148.5 announcement to be arranged), because the nanofiber filler that the PFSPE pillar adopts is different from the granulated filler that the SPE pillar uses, existing SPE extraction equipment is not suitable for the extracting operation of PFSPE pillar, so need be at PFSPE pillar exploiting economy, practicality, solid-phase extraction device efficiently.
Summary of the invention
Technical problem: the purpose of this invention is to provide a kind of array solid-phase extraction device based on nanofiber absorption.Can overcome shortcomings such as existing instrument costliness in the prior art, complicated operation are loaded down with trivial details, but and cost is lower, simple in structure, easy to install, save manpower batch process sample.
Technical scheme: the array solid-phase extraction device based on nanofiber absorption of the present invention comprises that syringe, bracing frame and steel press from both sides three parts, bracing frame is designed to rectangle or foursquare frame structure, totally 5 layers from the bottom to top, the bottom is a base, be placed with a waste liquid tank that can conveniently take out on the base, it on the waste liquid tank receiving tube support, upwards be a fixing solid phase extraction column support again, upwards be the fixing intubate frame of one deck again, end at the intubate frame is provided with connecting axle, is with a pressing plate that can slide up and down with left rotation and right rotation on the connecting axle; Syringe is fixed on the intubate frame, and the push rod end of syringe is connected with pressing plate, and the front end of syringe is provided with the extraction pillar; Small test tube is positioned on the receiving tube support, and the front end of extraction pillar passes the openend that the solid phase extraction column support is positioned at small test tube.Receiving tube support on the waste liquid tank is dismountable travel(l)ing rest.
Operation principle:
Medical syringe is put into the circular hole of intubate frame, the solid phase extraction column (in the patent No. is 200510123148.5 patent announcement being arranged) that is filled with nanofiber is inserted the solid phase extraction column support, adding is treated the sample of enrichment and is connected with syringe in pillar, with the steel folder pressing plate and intubate rack-layer are clamped again, when pressing down, gives by pressing plate air pressurized in the syringe, make the sample in the microtrabeculae slowly pass through the nanofiber pillar, treat that sample all flows through the back and add eluting solvent in pillar, the wash-out that pressurizes again, and in dismountable receiving tube support, put into small test tube and be used to collect eluent.
Beneficial effect: the design's the array solid-phase extraction device based on nanofiber absorption does not need the more expensive auxiliaries such as vavuum pump of configuration rates, and the auxiliary that is adopted all is common inexpensive.Use steel to press from both sides, can carry out post, washing and the wash-out excessively of sample fast to the syringe pressurization, easy to operation.But that this array solid-phase extraction device has is simple in structure, easy to install, save manpower batch process sample, handle short, characteristics such as cost is lower of sample time.
Description of drawings
Fig. 1 is the structural representation of array solid-phase extraction device.
Wherein have: bracing frame 1, steel folder 2, syringe 3, connecting axle 4, pressing plate 5, intubate frame 6, solid phase extraction column support 7, receiving tube support 8, waste liquid tank 9, extraction pillar 10, small test tube 11.
The specific embodiment
Array solid-phase extraction device based on nanofiber absorption of the present invention is a kind of sample pre-treatments device, this device is by syringe, bracing frame and steel press from both sides three parts to be formed, bracing frame is designed to rectangle or foursquare frame structure, totally 5 layers from the bottom to top, the bottom is the base of stainless steel, be placed with a waste liquid tank that can conveniently take out on the base, it on the waste liquid tank dismountable receiving tube support, upwards be a fixing solid phase extraction column support again, upwards be the fixing intubate frame of one deck again, end at the intubate frame is provided with connecting axle, is with one on the connecting axle and can slides up and down corrosion resistant plate with left rotation and right rotation as pressing plate.Whole device can be designed to array porous (horizontal, longitudinal extension, this paper schematic diagram is single-row 6 holes) type.
With the example of stabilizing among the human plasma sample that is determined as, the performance of array solid-phase extraction device is described.
6 solid phase extraction columns that are filled with nanofiber are inserted in the solid phase extraction column support, add 50 μ L methyl alcohol and 50 μ L water respectively in pillar, pillar and syringe are connected, clamp pressing plate and intubate rack-layer with steel, pressurization makes solvent cross post to clean behind the activation nanofiber stand-by.Get perchloric acid precipitation's albumen that the stable mark-on blood plasma of 0.5mL adds 0.1mL 40%, 10, get supernatant behind 000 * g high speed centrifugation and add the NaOH adjusting pH of an amount of 2mol/L to neutral, then with in the pillar that extremely activation is good of sample on the plasma sample of handling well, pressurize with method, when sample liquid flow through pillar, object was adsorbed on the nanofiber, and discard solution passes pillar and flows into waste liquid tank.After using 50 μ L water washing pillars again, put into the small test tube that is used to collect eluent in dismountable receiving tube support, with 50 μ L methanol-eluted fractions, eluent is collected in the small test tube, directly gets 20 μ L eluents and injects the high performance liquid chromatograph analysis.The extraction recovery of blood plasma mark-on stable (10ng/mL) can reach more than 80%, and sample can be removed chaff interferences a large amount of in the blood sample, and object has been concentrated 10 times after SPE is handled, and whole operation is simple, convenient, quick, laborsaving.
Claims (2)
1, a kind of array solid-phase extraction device based on nanofiber absorption, it is characterized in that this device comprises syringe (3), bracing frame (1) and steel folder (2) three parts, bracing frame (1) is designed to rectangle or foursquare frame structure, totally 5 layers from the bottom to top, the bottom is a base, be placed with a waste liquid tank that can conveniently take out (9) on the base, on the waste liquid tank (9) receiving tube support (8), upwards be a fixing solid phase extraction column support (7) again, upwards be the fixing intubate frame (6) of one deck again, end at intubate frame (6) is provided with connecting axle (4), is with a pressing plate (5) that can slide up and down with left rotation and right rotation on the connecting axle (4); Syringe (3) is fixed on the intubate frame (6), and the push rod end of syringe (3) is connected with pressing plate (5), and the front end of syringe (3) is provided with extraction pillar (10); Small test tube (11) is positioned on the receiving tube support (8), and the front end of extraction pillar (10) passes the openend that solid phase extraction column support (7) is positioned at small test tube (11).
2, the array solid-phase extraction device based on nanofiber absorption according to claim 1 is characterized in that the receiving tube support (8) on the waste liquid tank (9) is dismountable travel(l)ing rest.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100335593A CN101590333B (en) | 2009-06-23 | 2009-06-23 | Nanofiber adsorption based array solid-phase extraction device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100335593A CN101590333B (en) | 2009-06-23 | 2009-06-23 | Nanofiber adsorption based array solid-phase extraction device |
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| CN101590333A true CN101590333A (en) | 2009-12-02 |
| CN101590333B CN101590333B (en) | 2011-02-16 |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101947393A (en) * | 2010-08-23 | 2011-01-19 | 东南大学 | Split-type nanometer fibre solid-phase extraction column and application thereof |
| CN103007575A (en) * | 2012-12-27 | 2013-04-03 | 天津工业大学 | Rapid, simple and convenient solid phase extracting device applying functional non-woven fabric membrane |
| CN103033582A (en) * | 2013-01-10 | 2013-04-10 | 广东珠江桥生物科技股份有限公司 | Probe coated with SPME coatings and used for collecting toxic substances and application of probe |
| CN103191584A (en) * | 2013-04-26 | 2013-07-10 | 苏州东奇生物科技有限公司 | Portable solid phase extracting device |
| CN103263784A (en) * | 2013-04-26 | 2013-08-28 | 苏州东奇生物科技有限公司 | Micro-/nano- structure filler-based solid phase extractor |
| CN103920306A (en) * | 2014-04-15 | 2014-07-16 | 东南大学 | Pressurization device of nanofiber solid phase extraction column |
| CN104225958A (en) * | 2014-08-29 | 2014-12-24 | 康国华 | Pressurization solid phase extraction device |
| CN105149029A (en) * | 2015-10-20 | 2015-12-16 | 南通大学 | Magnetic force base injector support used in super clean bench |
| CN105879950A (en) * | 2014-12-05 | 2016-08-24 | 安顺市晶英科技研发有限责任公司 | Test tube clamping device |
| CN108827744A (en) * | 2018-04-24 | 2018-11-16 | 厦门大学 | Using integral material as the portable field sample preparation apparatus of spe medium |
| CN109443875A (en) * | 2018-12-10 | 2019-03-08 | 华南农业大学 | A kind of Solid Phase Extraction pressure aid device and system |
| CN109621633A (en) * | 2019-02-22 | 2019-04-16 | 中国农业科学院棉花研究所 | A kind of auxiliary device of adsorption column elution |
| CN109675343A (en) * | 2018-12-07 | 2019-04-26 | 天津迪沃特生物电子科技有限公司 | A kind of method and device extracted and/or purify |
| CN109709239A (en) * | 2019-01-28 | 2019-05-03 | 天津医科大学 | A kind of detection method using the online pre-treatment urine sample of Poly-crown ether functionalized nano-fiber |
| CN111443074A (en) * | 2020-05-11 | 2020-07-24 | 厦门大学 | Automatic on-line pretreatment and Raman detection device and method thereof |
| CN111751195A (en) * | 2020-06-29 | 2020-10-09 | 中国水产科学研究院 | Multi-channel automatic purification device |
Family Cites Families (4)
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| FR2583655B3 (en) * | 1985-06-24 | 1987-09-11 | Bionova Sarl | DEVICE FOR ADAPTING VARIOUS GEOMETRIES OF MULTI-WAY PIPETTING STATIONS |
| CN2117942U (en) * | 1991-08-30 | 1992-10-07 | 刘建元 | Combined continuous sample adder and moving test-tube stand |
| CN2384214Y (en) * | 1999-04-26 | 2000-06-21 | 中国刑事警察学院 | Injection vibrator |
| CN1330399C (en) * | 2005-12-19 | 2007-08-08 | 东南大学 | Solid phase micro-extraction device based on nanometer fiber |
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2009
- 2009-06-23 CN CN2009100335593A patent/CN101590333B/en not_active Expired - Fee Related
Cited By (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101947393A (en) * | 2010-08-23 | 2011-01-19 | 东南大学 | Split-type nanometer fibre solid-phase extraction column and application thereof |
| CN103007575A (en) * | 2012-12-27 | 2013-04-03 | 天津工业大学 | Rapid, simple and convenient solid phase extracting device applying functional non-woven fabric membrane |
| CN103033582A (en) * | 2013-01-10 | 2013-04-10 | 广东珠江桥生物科技股份有限公司 | Probe coated with SPME coatings and used for collecting toxic substances and application of probe |
| CN103191584B (en) * | 2013-04-26 | 2015-01-21 | 苏州东奇生物科技有限公司 | Portable solid phase extracting device |
| CN103191584A (en) * | 2013-04-26 | 2013-07-10 | 苏州东奇生物科技有限公司 | Portable solid phase extracting device |
| CN103263784A (en) * | 2013-04-26 | 2013-08-28 | 苏州东奇生物科技有限公司 | Micro-/nano- structure filler-based solid phase extractor |
| CN103263784B (en) * | 2013-04-26 | 2015-09-09 | 苏州东奇生物科技有限公司 | A kind of solid extractor based on micro-nano structure filler |
| CN103920306B (en) * | 2014-04-15 | 2015-11-04 | 东南大学 | A kind of pressue device for nano-fiber solid phase extraction column |
| CN103920306A (en) * | 2014-04-15 | 2014-07-16 | 东南大学 | Pressurization device of nanofiber solid phase extraction column |
| CN104225958A (en) * | 2014-08-29 | 2014-12-24 | 康国华 | Pressurization solid phase extraction device |
| CN104225958B (en) * | 2014-08-29 | 2016-04-06 | 康国华 | A kind of pressurization solid-phase extraction device |
| CN105879950B (en) * | 2014-12-05 | 2018-09-28 | 嘉兴维特拉电气科技有限公司 | a kind of test tube clamping device |
| CN105879950A (en) * | 2014-12-05 | 2016-08-24 | 安顺市晶英科技研发有限责任公司 | Test tube clamping device |
| CN105149029A (en) * | 2015-10-20 | 2015-12-16 | 南通大学 | Magnetic force base injector support used in super clean bench |
| WO2017067053A1 (en) * | 2015-10-20 | 2017-04-27 | 南通大学 | Syringe rack having magnetic base used for clean bench |
| CN108827744A (en) * | 2018-04-24 | 2018-11-16 | 厦门大学 | Using integral material as the portable field sample preparation apparatus of spe medium |
| CN109675343A (en) * | 2018-12-07 | 2019-04-26 | 天津迪沃特生物电子科技有限公司 | A kind of method and device extracted and/or purify |
| CN109443875A (en) * | 2018-12-10 | 2019-03-08 | 华南农业大学 | A kind of Solid Phase Extraction pressure aid device and system |
| CN109709239A (en) * | 2019-01-28 | 2019-05-03 | 天津医科大学 | A kind of detection method using the online pre-treatment urine sample of Poly-crown ether functionalized nano-fiber |
| CN109621633A (en) * | 2019-02-22 | 2019-04-16 | 中国农业科学院棉花研究所 | A kind of auxiliary device of adsorption column elution |
| CN111443074A (en) * | 2020-05-11 | 2020-07-24 | 厦门大学 | Automatic on-line pretreatment and Raman detection device and method thereof |
| CN111443074B (en) * | 2020-05-11 | 2021-08-31 | 厦门大学 | Automatic on-line pretreatment and Raman detection device and method thereof |
| CN111751195A (en) * | 2020-06-29 | 2020-10-09 | 中国水产科学研究院 | Multi-channel automatic purification device |
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