CN107081271B - Nano material multidimensional isolates and purifies system - Google Patents
Nano material multidimensional isolates and purifies system Download PDFInfo
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- CN107081271B CN107081271B CN201710426289.7A CN201710426289A CN107081271B CN 107081271 B CN107081271 B CN 107081271B CN 201710426289 A CN201710426289 A CN 201710426289A CN 107081271 B CN107081271 B CN 107081271B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07B—SEPARATING SOLIDS FROM SOLIDS BY SIEVING, SCREENING, SIFTING OR BY USING GAS CURRENTS; SEPARATING BY OTHER DRY METHODS APPLICABLE TO BULK MATERIAL, e.g. LOOSE ARTICLES FIT TO BE HANDLED LIKE BULK MATERIAL
- B07B15/00—Combinations of apparatus for separating solids from solids by dry methods applicable to bulk material, e.g. loose articles fit to be handled like bulk material
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Abstract
The present invention provides a kind of nano material multidimensional to isolate and purify system, comprising: film is separated by filtration module, realizes that big grain diameter nano particle is isolated and purified with the one-dimensional of small particle nano particle;Hollow-fibre membrane flow field-flow fractionation module, for further carrying out two dimensional separation by particle size to the one-dimensional big grain diameter nano particle isolated and purified;Size exclusion chromatograph separation module, for further carrying out two dimensional separation by particle size to the one-dimensional small particle nano material isolated and purified;Ion exchange mechansim module, the nano material that the partial size for obtaining after purification to two dimensional separation is identical but surface capping agents are different carry out three-dimensional by surface electrical behavior and isolate and purify;And automatic control module, realize the switching and control to the above modules.Hereby it is achieved that the three-dimensional of the nano material of different-grain diameter and different coverings cladding isolates and purifies, separative efficiency is high, applied widely and reproducible, easy to operate, cost is relatively low.
Description
Technical field
The invention belongs to environmental analytical chemistry, it is related to a kind of nano material multidimensional and isolates and purifies system.
Background technique
With going deep into nano materials research, the environmental effect and biosafety issues of nano material have caused extensively
Concern is in recent years to refer to about nano material itself poisonous effect and with the report for cooperateing with toxic effect that pollutant coexists
Number increases.The many factors such as the toxicity of nano material and its partial size, surface charge, coating material, surrounding medium are closely related,
How it is isolated and purified and is detected, is the key that the environmental behaviour and biological effect for evaluating nano material.It is common at present
Nano material isolation technics mainly include density gradient centrifugation, extraction, size exclusion chromatography, Capillary Electrophoresis, Field-Flow Fractionation
Deng.Centrifugation and extraction are mostly used for the separation between small molecule and nano particle, but point for the nano material being not much different to partial size
It is helpless from often;Size exclusion chromatography more subtly can sieve nano material by partial size, but often face chromatographic column damage
Bad the problems such as;Although Capillary Electrophoresis can subtly be sieved nano material by surface charge, operating cost is low, is unsuitable for a large amount of
The quick separating of sample;Field flow fractionation system can realize efficiently separating for 10nm~10 μm nano material, but to being less than
The particle screening of 10nm is relatively difficult, and for same particle size but the different nano material of covering, also without phase
The isolation technics answered.Therefore, widely distributed for size, the miscellaneous nano material of covering, the following technology of urgent need to resolve
Problem: the multidimensional for designing a kind of nano material isolates and purifies system, the nanometer that can coat each partial size and different coverings
Material realizes high efficiency separation, and with preferably repeated, easy to operate, cost is relatively low.
Summary of the invention
(1) technical problems to be solved
The present invention provides a kind of nano material multidimensional to isolate and purify system, at least partly to solve skill set forth above
Art problem.
(2) technical solution
According to an aspect of the invention, there is provided a kind of nano material multidimensional isolates and purifies system, comprising: film filtering point
From module 100, realize that big grain diameter nano particle is isolated and purified with the one-dimensional of small particle nano particle;Hollow-fibre membrane flow field stream
Separation module 210, for further carrying out two dimension point by particle size to the one-dimensional big grain diameter nano particle isolated and purified
From;Size exclusion chromatograph separation module 220, for the one-dimensional small particle nano material isolated and purified further by partial size
Size carries out two dimensional separation;Ion exchange mechansim module 300, the partial size for being obtained after purification to two dimensional separation it is identical but
The different nano material of surface capping agents carries out three-dimensional by surface electrical behavior and isolates and purifies;And automatic control module 400, realization pair
The switching and control of the above modules.
In one embodiment of the invention, film is separated by filtration module 100 by using different molecular weight cut off super filter tube,
To realize that big grain diameter nano particle and the one-dimensional of small particle nano particle isolate and purify.
In one embodiment of the invention, the split tunnel of hollow-fibre membrane flow field-flow fractionation module 210 is selected as follows
One or more of material: regenerated cellulose, polysulfones, polyether sulfone and polyacrylonitrile.
In one embodiment of the invention, size exclusion chromatograph separation module 220 selects the anti-of different pore size stationary phase
Phase chromatographic column.
In one embodiment of the invention, ion exchange mechansim module 300 is filled using anion exchange resin
Pillar.
In one embodiment of the invention, automatic control module 400 filters film using the software realization of independent development
Separation module 100, hollow-fibre membrane flow field-flow fractionation module 210, size exclusion chromatograph separation module 220 and ion exchange color
Compose the automatic control of separation module 300.
In one embodiment of the invention, hollow-fibre membrane flow field-flow fractionation module 210, size exclusion chromatograph splitting die
Block 220 is connected with ion exchange mechansim module 300 using peek pipe.
In one embodiment of the invention, the internal diameter of peek pipe is 0.25 μm.
In one embodiment of the invention, automatic control module 400 by the nano material multidimensional isolate and purify system with
Detection system 500 is combined, from hollow-fibre membrane flow field-flow fractionation module 210, size exclusion chromatograph separation module 220 and ion
The sample that these three modules of exchange chromatography separation module 300 are separated, which is introduced directly into detection system 500, to be analyzed and characterized.
In one embodiment of the invention, detection system 500 includes: laser particle size analyzer (DLS), ultraviolet-visible
Detector (UV-vis) and icp ms (ICP-MS).
(3) beneficial effect
It can be seen from the above technical proposal that nano material multidimensional provided by the invention isolates and purifies system, have following
The utility model has the advantages that
It has set gradually film and has been separated by filtration module, middle control tunica fibrosa flow field-flow fractionation module and size exclusion chromatograph splitting die
Block, ion exchange mechansim module, and the switching and control to the above modules are realized by automatic control module, it realizes
The three-dimensional of nano material of different-grain diameter and different coverings cladding isolates and purifies, and separative efficiency is high, applied widely and repeatedly
Property it is good, easy to operate, cost is relatively low, and can also be combined itself and detecting instrument, realize the analysis and characterization of nano material, grasp
Make easy.
Detailed description of the invention
Fig. 1 is the structural schematic diagram that system is isolated and purified according to nano material multidimensional of the embodiment of the present invention.
Fig. 2 is according to grain size distribution after nano silver membrane filtration separation module of the embodiment of the present invention, wherein (a) is super
Argent grain topographic profile figure before filter;It (b) is corresponding grading curve figure before ultrafiltration;It (c) is to be separated by filtration mould by film
After block ultrafiltration, the topographic profile figure of Argent grain;It (d) is corresponding grading curve figure after ultrafiltration.
Fig. 3 is according to size exclusion chromatograph module separation determination 1.6nm of embodiment of the present invention nano silver, 4.2nm nano-sulfur
Change the testing result figure of silver and 10nm nano silver.
[symbol description]
100- film is separated by filtration module;
210- hollow-fibre membrane flow field-flow fractionation module;
220- size exclusion chromatograph separation module;
300- ion exchange mechansim module;
400- automatic control module;
500- detection system.
Specific embodiment
The present invention provides a kind of nano material multidimensional to isolate and purify system, set gradually film be separated by filtration module, in
Tunica fibrosa flow field-flow fractionation module and size exclusion chromatograph separation module, ion exchange mechansim module are controlled, and by automatic
Control module realizes the switching and control to above-mentioned modules, realizes the nano material of different-grain diameter and different coverings cladding
Three-dimensional isolate and purify, separative efficiency is high, applied widely and reproducible, easy to operate, cost is relatively low, and can also will
It is combined with detecting instrument, realizes the analysis and characterization of nano material, easy to operate.
To make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with specific embodiment, and reference
Attached drawing, invention is further described in detail.
In an exemplary embodiment of the present invention, it provides a kind of nano material multidimensional and isolates and purifies system.Fig. 1 is
Nano material multidimensional according to embodiments of the present invention isolates and purifies the structural schematic diagram of system, as shown in Figure 1, nano material multidimensional point
From purification system, comprising:
Film is separated by filtration module 100, realizes that big grain diameter nano particle is isolated and purified with the one-dimensional of small particle nano particle;
Hollow-fibre membrane flow field-flow fractionation module 210, for the one-dimensional big grain diameter nano particle isolated and purified into
One step carries out two dimensional separation by particle size;
Size exclusion chromatograph separation module 220, for further to the one-dimensional small particle nano material isolated and purified
Two dimensional separation is carried out by particle size;
Ion exchange mechansim module 300, the partial size for obtaining after purification to two dimensional separation is identical but surface coats
The different nano material of agent carries out three-dimensional by surface electrical behavior and isolates and purifies;And
Automatic control module 400 realizes switching and control to above-mentioned modules.
The various pieces for isolating and purifying system to nano material multidimensional of the embodiment of the present invention below describe in detail.Fig. 1
Middle modules, which are adopted, to be indicated by the dashed box.
In the present embodiment, film is separated by filtration module 100 by using different molecular weight cut off super filter tube respectively, to realize greatly
Grain diameter nano particle is isolated and purified with the one-dimensional of small particle nano particle, and wherein small particle nano particle is nanocluster;
The material of the split tunnel of hollow-fibre membrane flow field-flow fractionation module 210 can choose regenerated cellulose, polysulfones, gather
Ether sulfone, polyacrylonitrile etc., the material of the preferred split tunnel of the embodiment of the present invention are polyacrylonitrile;Mobile phase is for conveying nanometer
Material, generally liquid phase;The separation process of the module is divided into two steps of focusing and elution, in focusing, plunger pump 1 and plunger
Liquid stream in pump 2 inputs inside hollow fibre from doughnut export and import respectively, after certain time (such as 3 minutes),
Plunger pump 1 is closed, opens elution mode, during elution, the nano material of different-grain diameter is sequentially entered according to size
Detection system, radial flow are discharged into waste liquid bottle, as shown in figure 1 shown in " waste liquid " part, wherein 2 corresponding diagram of plunger pump 1 and plunger pump
" pump 1 " and " pump 2 " in 1;
In the present embodiment, total volume exclusion chromatography separation module 220 selects the reverse-phase chromatographic column of different pore size stationary phase, note
Pump 3 is penetrated for the small particle nano material after one-dimensional separation to be sent into size exclusion chromatograph column, the component after separation enters detection
System realizes the two dimensional separation of different size small particle nano materials, wherein in 3 corresponding diagram 1 of syringe pump " pump 3 ";
In the present embodiment, ion exchange mechansim module 300 fills pillar, syringe pump 4 using anion exchange resin
For the nano material that the partial size after two dimensional separation is similar but surface charge is different to be sent into ion-exchange chromatography, after separation
Component enters detection system, further realizes three-dimensional separation, wherein in 4 corresponding diagram 1 of syringe pump " pump 4 ";
Automatic control module 400 is separated by filtration module 100, hollow fibre to above-mentioned film using the software realization of independent development
Tie up oneself of film flow field-flow fractionation module 210, size exclusion chromatograph separation module 220 and ion exchange mechansim module 300
Dynamic control;
The manual trace sampler for being sent into the nano material that nano material of embodiment of the present invention multidimensional isolates and purifies system is matched
There is quantitative loop;
In the present embodiment, hollow-fibre membrane flow field-flow fractionation module 210, size exclusion chromatograph separation module 220 and ion
Exchange chromatography separation module 300 is connected using 0.25 μm of internal diameter of peek pipe;
Above-mentioned nano material multidimensional can also be isolated and purified system and detection system 500 by automatic control module 400
Combination, the detection system include: laser particle size analyzer (DLS), UV-visible detector (UV-vis) and inductive coupling etc. from
The detecting instruments such as daughter mass spectrograph (ICP-MS), to directly be analyzed and be characterized the sample isolated and purified, eliminate by
Separate the trouble of the independent sample presentation detection of sample;Moreover, being separated from empty fiber membrane flow field-flow fractionation module 210, size exclusion chromatograph
The sample that module 220 and ion exchange mechansim module 300 these three modules are separated can be introduced directly into detection
It is analyzed and characterized in system;
The nano material multidimensional according to above-described embodiment isolates and purifies multidimensional separation, the purifying that system carries out particulate form silver
And measurement experiment.
After particulate form silver sample membrane filtration separation module carries out one-dimensional separation, certain volume, such as the sample of 20 μ L are taken
Through being furnished with the manual trace sampler sample introduction of quantitative loop, successively pass through hollow-fibre membrane flow field-flow fractionation module, volume exclusion color
Compose separation module and ion exchange mechansim module, in hollow-fibre membrane flow field-flow fractionation module, plunger pump 1 and plunger pump
Liquid stream in 2 inputs inside hollow fibre from doughnut export and import respectively, wherein 2 corresponding diagram of plunger pump 1 and plunger pump
In 1 " pump 1 " and " pump 2 ";After 3 minutes, plunger pump 1 is closed, opens elution mode, during elution, different-grain diameter nano silver
Detection system is sequentially entered by size, radial flow is discharged into waste liquid bottle, as shown in figure 1 shown in " waste liquid " part;It is arranged in volume
Chromatographic isolation module is hindered, the small particle nano silver after one-dimensional separation is sent into size exclusion chromatograph column, receiving after separation by syringe pump 3
Rice silver components enter detection system, and syringe pump 3 here is corresponding in Fig. 1 " pump 3 ";In ion exchange mechansim module, note
It penetrates pump 4 and the partial size after two dimensional separation is similar but surface charge is different nano silver is sent into ion-exchange chromatography, in corresponding diagram 1
" pump 4 ", the nano silver of two kinds of coverings cladding after separation enters detection system, and the final multidimensional for realizing particulate form silver separates
Purifying and analysis and characterization.
Fig. 2 is according to grain size distribution after nano silver membrane filtration separation module of the embodiment of the present invention;Fig. 3 is according to this
Inventive embodiments size exclusion chromatograph module separation determination 1.6nm nano silver, 4.2nm silver sulfide nanometer particles and 10nm nano silver
Testing result figure.As a result, analyzing function that each module is realized and effect is as follows referring to shown in Fig. 2 and Fig. 3:
Film is separated by filtration module: using molecular cut off be 300kD super filter tube (Amicon Ultra-15,
Millipore, MA, USA), it can be achieved that partial size is less than 10nm and greater than one-dimensional point of the ag nano-cluster of 10nm and nano particle
From purifying;Its comparative result figure for separating front and back is as shown in Figure 2;Wherein, (a) is the Argent grain topographic profile figure before ultrafiltration;(b)
For grading curve figure corresponding before ultrafiltration;It (c) is the topographic profile of Argent grain after film is separated by filtration module ultrafiltration
Figure;It (d) is corresponding grading curve figure after ultrafiltration;As shown in Figure 2, the separating and filtering of non-membrane filtration separation module, sample
Product particulate form silver particle diameter distribution is at random, and the partial size including various sizes, is separated by filtration after the separating and filtering of module by film,
Nano-Ag particles of the partial size less than 10nm are retained;
Hollow-fibre membrane flow field-flow fractionation module: this hollow-fibre membrane flow field-flow fractionation device is used, with FL-70
(0.1%, m/v) and sodium azide (0.02%, m/v) mixed solution are mobile phase, are realized through the one-dimensional grain isolated and purified
The two dimensional separation purifying of nano particle of the diameter greater than 10nm;
Size exclusion chromatograph separation module: the chromatographic column of 500 aperture stationary phases of selection, realization are isolated and purified through one-dimensional
The partial size arrived is less than the nano particle of 10nm, is that the two dimensional separation of 1.6nm nano silver and 4.2nm silver sulfide nanometer particles is pure in this experiment
Change;Testing result after separation is as shown in figure 3, from the figure 3, it may be seen that be less than receiving for 10nm by the one-dimensional partial size isolated and purified
The curve of the top in rice grain corresponding diagram 3, substance is 1.6nm nano-Ag particles and 2nm silver sulfide nanometer particles particle, by body
Product exclusion chromatography separation module carries out two dimensional separation after purification, respectively obtains 4.2nm silver sulfide nanometer particles and 1.6nm nano silver, point
The curve of curve and bottom among other corresponding diagram 3, realizes the two dimensional separation of different-grain diameter particle;
Ion exchange mechansim module: using Ambelite IRN-78 anion exchange resin filling pillar, it can be achieved that
Partial size is identical, but the three-dimensional of the different two kinds of nano silvers of surface capping agents isolates and purifies, the partial size of two kinds of nano silvers in this experiment
It is 10nm, surface capping agents are respectively polyvinylpyrrolidone and citric acid, the experimental results showed that by ion-exchange chromatography
The separation of above two nano silver may be implemented in separation module.
In conclusion the embodiment of the invention provides a kind of nano material multidimensional to isolate and purify system, film has been set gradually
It is separated by filtration module, middle control tunica fibrosa flow field-flow fractionation module and size exclusion chromatograph separation module, ion exchange mechansim
Module, and the switching and control to above-mentioned modules are realized by automatic control module, realize different-grain diameter and different claddings
The three-dimensional of nano material of agent cladding isolates and purifies, and separative efficiency is high, applied widely and reproducible, easy to operate, cost
It is lower, and it can also be combined with detecting instrument, realize the analysis and characterization of nano material, it is easy to operate.
It should be noted that running through attached drawing, identical element is indicated by same or similar appended drawing reference.In the following description,
Some specific embodiments are used for description purposes only, and should not be construed to the present invention has any restrictions, and only the present invention is real
Apply the example of example.When may cause the understanding of the present invention and cause to obscure, conventional structure or construction will be omitted.It should be noted that figure
In the shape and size of each component do not reflect actual size and ratio, and only illustrate the content of the embodiment of the present invention.
Certainly, according to actual needs, nano material multidimensional provided by the invention isolates and purifies system, also comprising other normal
With structure and composition, since the innovation of same invention is unrelated, details are not described herein again.
Particular embodiments described above has carried out further in detail the purpose of the present invention, technical scheme and beneficial effects
Describe in detail it is bright, it should be understood that be not intended to restrict the invention the foregoing is merely the specific embodiment of invention, it is all
Within the spirit and principles in the present invention, any modification, equivalent substitution, improvement and etc. done should be included in protection of the invention
Within the scope of.
Claims (9)
1. a kind of nano material multidimensional isolates and purifies system, comprising:
Film is separated by filtration module (100), realizes that big grain diameter nano particle is isolated and purified with the one-dimensional of small particle nano particle;
Hollow-fibre membrane flow field-flow fractionation module (210), for the one-dimensional big grain diameter nano particle isolated and purified into one
Step carries out two dimensional separation by particle size;
Size exclusion chromatograph separation module (220), for the one-dimensional small particle nano material isolated and purified further by
Particle size carries out two dimensional separation;
Ion exchange mechansim module (300), the partial size for obtaining after purification to two dimensional separation is identical but surface capping agents
Different nano materials carries out three-dimensional by surface electrical behavior and isolates and purifies;And
Automatic control module (400) realizes switching and control to the modules.
2. nano material multidimensional according to claim 1 isolates and purifies system, wherein the film is separated by filtration module
(100) by using different molecular weight cut off super filter tube, to realize the one-dimensional of big grain diameter nano particle and small particle nano particle
It isolates and purifies.
3. nano material multidimensional according to claim 1 isolates and purifies system, wherein hollow-fibre membrane flow field flow point
Split tunnel from module (210) selects one or more of following material: regenerated cellulose, polysulfones, polyether sulfone and poly- third
Alkene nitrile.
4. nano material multidimensional according to claim 1 isolates and purifies system, wherein the size exclusion chromatograph splitting die
The reverse-phase chromatographic column of block (220) selection different pore size stationary phase.
5. nano material multidimensional according to claim 1 isolates and purifies system, wherein the ion exchange mechansim mould
Block (300) fills pillar using anion exchange resin.
6. nano material multidimensional according to claim 1 isolates and purifies system, wherein hollow-fibre membrane flow field flow point
Peek pipe phase is used from module (210), size exclusion chromatograph separation module (220) and ion exchange mechansim module (300)
Connection.
7. nano material multidimensional according to claim 6 isolates and purifies system, wherein the internal diameter of the peek pipe is 0.25
μm。
8. nano material multidimensional according to any one of claims 1 to 7 isolates and purifies system, the automatic control module
(400) the nano material multidimensional is isolated and purified into system and detection system (500) is combined, from hollow-fibre membrane flow field-flow fractionation
Module (210), size exclusion chromatograph separation module (220) and ion exchange mechansim module (300) these three modules point
The sample separated out, which is introduced directly into the detection system (500), to be analyzed and characterized.
9. nano material multidimensional according to claim 8 isolates and purifies system, wherein the detection system (500) includes:
Laser particle size analyzer, UV-visible detector and icp ms.
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CN108333016A (en) * | 2017-11-08 | 2018-07-27 | 中国科学院生态环境研究中心 | The method of trace metal and its compound nano-material in separation and concentration water body |
CN109966780B (en) * | 2019-03-21 | 2021-03-09 | 浙江工商大学 | Method for separating functional nanoparticles from aquatic shellfish cooking soup |
CN111214958B (en) * | 2020-01-17 | 2022-07-19 | 中山大学 | Method for extracting soil rare earth nanoparticles |
CN111715877B (en) * | 2020-05-22 | 2022-06-03 | 深圳市华科创智技术有限公司 | Method for purifying nano silver wire |
CN113125365B (en) * | 2021-04-16 | 2022-05-13 | 中国科学院生态环境研究中心 | Device and method for quantitatively identifying nano zinc oxide and zinc ions in solution |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101484234A (en) * | 2006-05-06 | 2009-07-15 | 门布拉内有限公司 | Ultrafiltration membrane |
CN101948896A (en) * | 2010-09-02 | 2011-01-19 | 天津大学 | Integrated preparation method of multi-function milk casein active peptides |
CN104155381A (en) * | 2014-08-21 | 2014-11-19 | 中国科学院生态环境研究中心 | Method for rapidly separating and measuring nano silver and silver ions |
CN104297369A (en) * | 2014-09-28 | 2015-01-21 | 中国科学院生态环境研究中心 | Device and method for determining nanometer material through hollow fiber field-flow fractionation |
CN104614435A (en) * | 2015-02-06 | 2015-05-13 | 中国科学院生态环境研究中心 | Method for separating and measuring nano-silver and silver ions |
CN104777081A (en) * | 2015-04-01 | 2015-07-15 | 上海交通大学 | Nano particle separation and detection method |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5070442B2 (en) * | 2002-02-28 | 2012-11-14 | 株式会社Phg | Novel polypeptide and method for producing the same |
WO2012109615A1 (en) * | 2011-02-10 | 2012-08-16 | Life Technologies Corporation | Purification systems and methods |
-
2017
- 2017-06-07 CN CN201710426289.7A patent/CN107081271B/en active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101484234A (en) * | 2006-05-06 | 2009-07-15 | 门布拉内有限公司 | Ultrafiltration membrane |
CN101948896A (en) * | 2010-09-02 | 2011-01-19 | 天津大学 | Integrated preparation method of multi-function milk casein active peptides |
CN104155381A (en) * | 2014-08-21 | 2014-11-19 | 中国科学院生态环境研究中心 | Method for rapidly separating and measuring nano silver and silver ions |
CN104297369A (en) * | 2014-09-28 | 2015-01-21 | 中国科学院生态环境研究中心 | Device and method for determining nanometer material through hollow fiber field-flow fractionation |
CN104614435A (en) * | 2015-02-06 | 2015-05-13 | 中国科学院生态环境研究中心 | Method for separating and measuring nano-silver and silver ions |
CN104777081A (en) * | 2015-04-01 | 2015-07-15 | 上海交通大学 | Nano particle separation and detection method |
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