CN220237825U - Syringe type sieve-plate-free solid phase extraction column - Google Patents
Syringe type sieve-plate-free solid phase extraction column Download PDFInfo
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- CN220237825U CN220237825U CN202321230485.4U CN202321230485U CN220237825U CN 220237825 U CN220237825 U CN 220237825U CN 202321230485 U CN202321230485 U CN 202321230485U CN 220237825 U CN220237825 U CN 220237825U
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- 238000002414 normal-phase solid-phase extraction Methods 0.000 title claims abstract description 69
- 239000007788 liquid Substances 0.000 claims abstract description 87
- 238000001179 sorption measurement Methods 0.000 claims abstract description 44
- 238000000605 extraction Methods 0.000 claims abstract description 40
- 239000012071 phase Substances 0.000 claims abstract description 24
- 239000002699 waste material Substances 0.000 claims description 12
- 238000009434 installation Methods 0.000 claims description 7
- 238000012360 testing method Methods 0.000 claims description 7
- 230000006978 adaptation Effects 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 239000012190 activator Substances 0.000 claims description 3
- 239000012156 elution solvent Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 10
- 238000004140 cleaning Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 17
- 239000003463 adsorbent Substances 0.000 description 14
- 238000010586 diagram Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 4
- 239000004810 polytetrafluoroethylene Substances 0.000 description 3
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000005465 channeling Effects 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
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- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The utility model discloses a syringe type sieve-plate-free solid phase extraction column, which is characterized in that: the liquid extraction column comprises an extraction column body, wherein a solution section and an adsorption section are connected in a split mode through a threaded structure, the solution section can be used for selecting a proper height model according to the scale of a sample, the adsorption section is internally filled with a replaceable sieve-plate-free structure adsorption phase, the extraction efficiency is improved while the structure of the adsorption section is compact, a solid-phase extraction device is matched with the adsorption section, a positive pressure device, a negative pressure device, a positive pressure interface valve, a negative pressure interface valve, a liquid inlet valve, a liquid outlet valve and a pressure gauge can keep the pressure in the solid-phase extraction column stable in operation, so that the flow rate in the operation process is easy to control, the excessive problem of liquid inlet can be solved and the self-cleaning function is realized through the matched use of the equipment, the operation is completed in a closed space, the condition of solution pollution and liquid leakage can be avoided, and the operation safety is effectively improved.
Description
Technical Field
The utility model relates to the technical field of analytical chemistry, in particular to a syringe type sieve-plate-free solid-phase extraction column.
Background
The solid phase extraction column is a sample pretreatment device developed from a chromatographic column for extraction, separation and concentration. The method is mainly applied to sample pretreatment of target compounds in various foods, agricultural and animal products, environmental samples and biological samples.
The specifications of the solid phase extraction column of the traditional syringe type solid phase extraction column can be divided into 1mL, 3mL, 6mL, 10mL, 15mL, 20mL, 30mL, 60mL and the like, the solid phase extraction column is applied according to specific extraction amount and extract, and the SPE column without a sieve plate is an integrated novel SPE column. The product adopts the process to carry out special treatment on the bonded silica gel adsorption phase such as C18, C8 and the like, so that the bonded silica gel adsorption phase has higher mechanical strength and toughness, a polyethylene porous baffle is not required to be added into a column to be used as a support, dead volume between adsorbents can be reduced, no ditch flow is formed during extraction, extraction efficiency is improved, the condition that the current syringe type phase extraction column has different adsorption phases according to different sample characteristics, so that the consumption of instruments in a laboratory and the storage capacity are larger is caused, meanwhile, the current syringe type solid phase extraction column generally adopts the manual operation mode, on one hand, the operation efficiency is low, and on the other hand, the conditions of solution pollution and liquid leakage are also easy to cause.
Disclosure of Invention
The utility model aims to overcome the defects in the prior art and provide the solid-phase extraction column with the syringe type and no sieve plate, which has reasonable structural design.
The utility model solves the problems by adopting the following technical scheme: the utility model provides a syringe type does not have sieve solid phase extraction post, includes the extraction post body, the extraction post body include solution section and adsorption phase, solution section and adsorption phase adopt the helicitic texture to carry out the adaptation, the solid phase extraction post be provided with solid phase extraction device, solid phase extraction device lateral wall and bottom make by transparent glass, above-mentioned solid phase extraction device top is provided with the apron, the apron be provided with multiunit mounting hole, the mounting hole pass through helicitic texture and connect liquid outlet valve or shutoff stopper, above-mentioned apron installation contact surface is provided with the sealing washer.
Further: the inside of adsorption section be filled with the adsorption phase, according to the different extraction application of adsorption phase, the extraction column body is provided with multiple model alternative.
Further: the solid-phase extraction device inside be provided with the baffle, the baffle with device inside divide into first cavity and second cavity, wherein first cavity is provided with the test-tube rack and is fixed to the baffle lateral part, the test-tube rack be provided with a plurality of hole sites, the second cavity in be provided with the waste liquid groove.
Further: the device is characterized in that a negative pressure connector is arranged below the solid-phase extraction device, a pressure gauge is arranged on the negative pressure connector, and the negative pressure connector is connected with the negative pressure device through a hose.
Further: the solution section include the feed liquor valve of being connected with the upper shed through the hose, feed liquor valve upper shed be connected with the feed liquor joint lower shed, the feed liquor joint top be provided with first inlet, second inlet, third inlet and fourth inlet, first inlet be connected to the liquid reserve tank activator liquid outlet, the second inlet be connected to liquid reserve tank sample liquid outlet, the third inlet be connected to liquid reserve tank drip washing solvent liquid outlet, the fourth inlet be connected to liquid reserve tank elution solvent liquid outlet.
Further: the adsorption section include the play liquid valve of being connected through hose and lower mouth, play liquid valve set to two-position three way reversing valve, play liquid valve lower mouth and play liquid joint upper shed connection, play liquid joint lower shed be provided with two interfaces, two interfaces pass through hose connection, the other end of the hose of connecting one of them interface leads to the test tube, the other end of the hose of connecting another interface leads to the waste liquid groove.
Compared with the prior art, the utility model has the following advantages and effects:
(1) In the product, the solution section and the adsorption section are connected in a split mode, in actual extraction operation, an operator can select a solution section with a proper height model according to the scale of a sample, and an adsorption phase is filled in the adsorption section, wherein the adsorption phase is prepared by a special process of mixing PTFE and the adsorbent and pressing the mixture into a wafer (namely, a sieve plate-free structure), dead volume among the adsorbents can be reduced, channeling is avoided during extraction, extraction efficiency is improved, and the structure of the adsorption section is ensured to be compact. According to the different extraction applications of the adsorption phase, the extraction column body is provided with various types for selection. In actual extraction operation, the operator can adopt the solution section and the adsorption section of suitable specification to connect the adaptation as required to realize the solid-phase extraction purpose of different sample capacities and target compounds, through selecting suitable adsorption phase scheme, when improving actual extraction effect, also can reduce the spare part of experimental equipment, thereby save the storage space of experimental equipment, reduce experimental storage cost.
(2) The side wall and the bottom of the solid phase extraction device matched with the product are made of transparent glass, so that operators can observe the internal condition of the device conveniently, and the visualization of the operation process is realized. Above-mentioned solid phase extraction device top structure is provided with the apron, the apron be provided with multiunit mounting hole, the test-tube rack of its cavity inside is provided with a plurality of hole sites, consequently can realize multiunit sample simultaneous operation to can improve operating efficiency by a wide margin. The separator is arranged in the solid phase extraction device, and divides the device into a first cavity and a second cavity, so that the solutions in the two cavities are not in direct contact, and pollution caused by the solutions is avoided. The sealing ring is further arranged on the installation contact surface of the cover plate, so that the tightness of the inside of the solid-phase extraction device after installation is guaranteed. Above-mentioned solid-phase extraction device below is provided with the negative pressure connector, the negative pressure connector be provided with the manometer, the negative pressure connector passes through the hose and is connected with the negative pressure device, when using the solid-phase extraction post to carry out sample pretreatment, can effectively compress the space of adsorption phase with the negative pressure, reduces dead volume's formation in the extraction process, can accelerate the moving speed of liquid phase, and then accelerates the absorption rate between sample and the adsorption phase, improves the utilization ratio and the extraction efficiency of adsorbent.
(3) The operator can connect the water pump through quick-operation joint, then close the feed liquor valve and open negative pressure device, introduce a certain amount of warm water and solution. After a certain time of washing, separating, filtering and other procedures, dead angles and dirt of the adsorbent in the solid phase extraction column can be fully removed, so that the adsorption performance of the column is continuously improved, and the extraction efficiency of a sample is improved. The solid phase extraction column obtained by the operation can be recycled for a plurality of times, so that the experimental cost is effectively saved, and the experimental cost is reduced. Meanwhile, dead areas and impurities in the adsorbent are cleaned after the adsorbent is recycled for multiple times, so that stability and consistency of extraction effects can be guaranteed for samples of the same type, and good repeatability is achieved. In addition, compared with the disposable solid phase extraction column, the recycled solid phase extraction column can reduce post-treatment steps and avoid environmental pollution caused by wastes. All the solid phase extraction operations are carried out in a closed environment, so that the conditions of solution pollution and liquid leakage can be avoided, and the operation safety is effectively improved.
Drawings
Fig. 1 is a schematic diagram of a solid phase extraction column assembly without a sieve plate of a syringe according to an embodiment of the utility model.
Fig. 2 is a schematic structural diagram of a syringe-type sieve-plate-free solid phase extraction column according to an embodiment of the utility model.
Fig. 3 is a schematic diagram of a solution section of a solid phase extraction column without a sieve plate of a syringe according to an embodiment of the present utility model.
Fig. 4 is a schematic diagram showing connection of liquid inlet connectors of a solid phase extraction column without a sieve plate of a syringe according to an embodiment of the utility model.
FIG. 5 is a schematic diagram of a solid phase extraction device according to an embodiment of the present utility model.
Figure number: the solid-phase extraction device comprises an operating platform 1, a solid-phase extraction device 2, a cover plate 201, a partition plate 202, a test tube rack 203, a negative pressure connection port 204, a pressure gauge 205, a storage rack 3, a liquid storage tank 4, a negative pressure device 5, a positive pressure device 6, a solid-phase extraction column 7, a solution section 701, a column 702, an adsorption section 703, an adsorption phase 704, a threaded connection structure 705, a liquid inlet valve 8, a liquid inlet connector 9, a first liquid inlet 901, a second liquid inlet 902, a third liquid inlet 903, a fourth liquid inlet 904, a cleaning port 905, a liquid outlet valve 10, a liquid outlet connector 11, a test tube 12 and a waste liquid tank 13.
Detailed Description
The present utility model will be described in further detail by way of examples with reference to the accompanying drawings, which are illustrative of the present utility model and not limited to the following examples.
Referring to fig. 1-5, the solid phase extraction column 7 with no sieve plate in the syringe type of the present embodiment includes an extraction column body, the extraction column body includes a solution section 701 and an adsorption section 703, and the solution section 701 and the adsorption end are adapted by adopting a thread structure. In the product, the solution section 701 and the adsorption section 703 are connected in a split manner, in the actual extraction operation, an operator can select a solution section 701 with a proper height model according to the sample scale, and the adsorption section 703 is filled with an adsorption phase 704, wherein the adsorption phase 704 is prepared by a special process of mixing PTFE and an adsorbent and pressing the PTFE and the adsorbent into a wafer (i.e. a sieve plate-free structure), so that dead volume among the adsorbents can be reduced, channeling is avoided during extraction, extraction efficiency is improved, and the structure of the adsorption section 703 is ensured to be compact. The extraction column body is provided with a variety of types for selection depending on the extraction application of the adsorbent phase 704. In actual extraction operation, an operator can adopt a solution section 701 and an adsorption section 703 with proper specifications to carry out connection adaptation according to requirements, so that the purposes of solid-phase extraction of different sample capacities and target compounds are achieved, and the proper adsorption phase 704 scheme is selected, so that the actual extraction effect is improved, the spare parts of experimental equipment can be reduced, the storage space of the experimental equipment is saved, and the experimental storage cost is reduced.
In this embodiment, the solid phase extraction column 7 is provided with a solid phase extraction device 2, and the side wall and the bottom of the solid phase extraction device 2 are made of transparent glass, so that an operator can observe the internal condition of the device conveniently, and the operation process is visualized. The top of the solid phase extraction device 2 is provided with a cover plate 201, the cover plate 201 is provided with a plurality of groups of mounting holes, and the mounting holes are connected with the liquid outlet valve 10 or the plugging plug through a threaded structure. In addition, the inside baffle 202 that is provided with of solid-phase extraction device 2, the baffle 202 with the device inside divide into first cavity and second cavity, wherein first cavity is provided with test-tube rack 203 and is fixed to baffle 202 lateral part, test-tube rack 203 be provided with a plurality of hole sites, can place corresponding quantity of test tube 12 according to the experiment needs, the second cavity in be provided with waste liquid groove 13 for solution in two cavities does not direct contact, thereby avoid the solution to cause the pollution. In the solid phase extraction operation process, according to the group number of the solid phase extraction columns 7, liquid outlet valves 10 with corresponding numbers are respectively installed on a cover plate 201, the rest installation holes are plugged by plugging plugs, after the liquid outlet valves 10 are connected with hoses, the connected hoses are divided into two groups, one group of ports are placed in corresponding test tubes 12, the other group of ports are placed above a waste liquid tank 13, after the device is installed and positioned, the device is installed on a solid phase extraction device 2 together with the cover plate 201, a sealing ring is arranged on an installation contact surface of the cover plate 201, and the tightness of the inside of the solid phase extraction device 2 after the installation is ensured. The solid-phase extraction device 2 below be provided with negative pressure connector 204, negative pressure connector 204 be provided with manometer 205, negative pressure connector 204 is connected with negative pressure device 5 through the hose, when using solid-phase extraction post 7 to carry out sample pretreatment, can effectively compress the space of adsorbing phase 704 with the negative pressure, reduce dead volume's formation in the extraction process, can accelerate the moving speed of liquid phase, and then accelerate the absorption rate between sample and the adsorbing phase 704, improve the utilization ratio and the extraction efficiency of adsorbent.
In this embodiment, the solution section 701 includes a liquid inlet valve 8 connected to an upper port through a hose, the upper port of the liquid inlet valve 8 is connected to a lower port of a liquid inlet connector 9, a first liquid inlet 901, a second liquid inlet 902, a third liquid inlet 903 and a fourth liquid inlet 904 are provided at the top of the liquid inlet connector 9, the first liquid inlet 901 is connected to an activator liquid outlet of the liquid storage tank 4, the second liquid inlet 902 is connected to a sample liquid outlet of the liquid storage tank 4, the third liquid inlet 903 is connected to a solvent liquid outlet of the liquid storage tank 4, the fourth liquid inlet 904 is connected to a solvent liquid outlet of the liquid storage tank 4, the adsorption section 703 includes a liquid outlet valve 10 connected to the lower port through a hose, the liquid outlet valve 10 is provided as a two-position three-way reversing valve, the lower port of the liquid outlet valve 10 is connected to the upper port of the liquid outlet connector 11, the lower port of the liquid outlet connector 11 is provided with two ports, the two ports are connected through a hose, one port is connected to the other port of the liquid storage tank 4, and the other port is connected to the other port 13 of the waste liquid tank through another hose. The operator controls the flow direction of the solution through the liquid outlet valve 10, and discharges the activated and sampled waste liquid after leaching to the waste liquid tank 13, and the eluted target compound solution is injected into the test tube 12.
In this embodiment, the liquid inlet valve 8 still be provided with clean mouth 905, clean mouth 905 accessible quick-operation joint connects the water pump, after finishing the connection, operator control liquid inlet valve 8 closes, opens negative pressure device 5, opens the water pump and introduces a certain amount of warm water and solution, the procedure such as washing, separation and filtration of passing a certain time makes dead angle and the filth of the inside adsorbent of solid-phase extraction post 7 fully clear away for the adsorption performance of post is continuously improved, thereby has improved the extraction efficiency of sample. The solid phase extraction column 7 that so gets down can recycle many times, practices thrift the cost in the experiment effectively, reduces experimental expense, and in addition after many times recycle the inside dead zone of its adsorbent, impurity etc. are got rid of, to the sample of same type, can guarantee the stability and the uniformity of extraction effect, possess better repeatability. In addition, compared with the disposable solid phase extraction column 7, the recycled solid phase extraction column 7 can reduce post-treatment steps and avoid environmental pollution caused by waste. All the solid phase extraction operations are carried out in a closed environment, so that the conditions of solution pollution and liquid leakage can be avoided, and the operation safety is effectively improved.
The foregoing description of the utility model is merely exemplary of the utility model. Those skilled in the art may make various modifications or additions to the described embodiments or substitutions, without departing from the scope of the utility model as defined in the accompanying claims.
Claims (6)
1. A syringe type solid phase extraction column without sieve plate is characterized in that: including the extraction column body, the extraction column body include solution section and adsorption section, solution section and adsorption terminal adopt the helicitic texture to carry out the adaptation, the solid phase extraction column be provided with solid phase extraction device, solid phase extraction device lateral wall and bottom be made by transparent glass, above-mentioned solid phase extraction device top is provided with the apron, the apron be provided with multiunit mounting hole, the mounting hole pass through helicitic texture and connect drain valve or shutoff stopper, above-mentioned apron installation contact surface is provided with the sealing washer.
2. The syringe type sieve-plate-free solid phase extraction column according to claim 1, wherein: the inside of adsorption section be filled with the adsorption phase, according to the different extraction application of adsorption phase, the extraction column body is provided with multiple model alternative.
3. The syringe type sieve-plate-free solid phase extraction column according to claim 2, wherein: the solid-phase extraction device inside be provided with the baffle, the baffle with device inside divide into first cavity and second cavity, wherein first cavity is provided with the test-tube rack and is fixed to the baffle lateral part, the test-tube rack be provided with a plurality of hole sites, the second cavity in be provided with the waste liquid groove.
4. The syringe type sieve-plate-free solid phase extraction column according to claim 2, wherein: the device is characterized in that a negative pressure connector is arranged below the solid-phase extraction device, a pressure gauge is arranged on the negative pressure connector, and the negative pressure connector is connected with the negative pressure device through a hose.
5. The syringe type sieve-plate-free solid phase extraction column according to claim 1, wherein: the solution section include the feed liquor valve of being connected with the upper shed through the hose, feed liquor valve upper shed be connected with the feed liquor joint lower shed, the feed liquor joint top be provided with first inlet, second inlet, third inlet and fourth inlet, first inlet be connected to the liquid reserve tank activator liquid outlet, the second inlet be connected to liquid reserve tank sample liquid outlet, the third inlet be connected to liquid reserve tank drip washing solvent liquid outlet, the fourth inlet be connected to liquid reserve tank elution solvent liquid outlet.
6. A syringe type sieve-plate-free solid phase extraction column according to any one of claims 1 to 5, wherein: the adsorption section include the play liquid valve of being connected through hose and lower mouth, play liquid valve set to two-position three way reversing valve, play liquid valve lower mouth and play liquid joint upper shed connection, play liquid joint lower shed be provided with two interfaces, two interfaces pass through hose connection, the other end of the hose of connecting one of them interface leads to the test tube, the other end of the hose of connecting another interface leads to the waste liquid groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321230485.4U CN220237825U (en) | 2023-05-18 | 2023-05-18 | Syringe type sieve-plate-free solid phase extraction column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321230485.4U CN220237825U (en) | 2023-05-18 | 2023-05-18 | Syringe type sieve-plate-free solid phase extraction column |
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| Publication Number | Publication Date |
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| CN220237825U true CN220237825U (en) | 2023-12-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202321230485.4U Active CN220237825U (en) | 2023-05-18 | 2023-05-18 | Syringe type sieve-plate-free solid phase extraction column |
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| CN (1) | CN220237825U (en) |
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- 2023-05-18 CN CN202321230485.4U patent/CN220237825U/en active Active
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