CN207958198U - Separation of Proteins extraction element - Google Patents
Separation of Proteins extraction element Download PDFInfo
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- CN207958198U CN207958198U CN201721861787.6U CN201721861787U CN207958198U CN 207958198 U CN207958198 U CN 207958198U CN 201721861787 U CN201721861787 U CN 201721861787U CN 207958198 U CN207958198 U CN 207958198U
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- collecting tank
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- storage room
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Abstract
The utility model discloses a kind of Separation of Proteins extraction element, it is made of three batch can, liquid collecting tank and chromatographic column parts, the liquid outlet of first batch can is connected with the second batch can by the first catheter, and the waste liquid port of first batch can is connected with the first liquid collecting tank by third catheter；The dialysis membrane being arranged in second batch can is classified as two storage rooms, and upper storage room is connected by the second catheter with third batch can, and lower storage room is connected by the 4th catheter with the second liquid collecting tank；The molecular sieve being arranged in third batch can is classified as two protein storage rooms, upper protein storage room is connected by the 5th catheter with the second chromatography column top, second chromatography column bottom is connected with the 4th liquid collecting tank, lower protein storage room bottom opening is inserted into the first chromatography column top, and first chromatography column bottom is connected with third liquid collecting tank.The utility model is on the basis of keeping protein active, high efficiency, low energy consumption, without adding any chemical reagents.
Description
Technical field
The utility model belongs to biotechnology more particularly to a kind of device for Separation of Proteins extraction.
Background technology
The separation and Extraction of protein is a challenging job, and separation is mainly big according to the molecule of protein
Small, solubility, adsorption property, carries out the aspect of biology affinity five of ligand molecular at charge.
Under normal conditions, protein is all mixture complex in composition, and some components itself are unstable in mixture, in advance
Heat meets certain yielding inactivations of solvent, therefore the isolation technics such as traditional distillation, solvent extraction are not particularly suited for it and isolate and purify.
In addition, often containing impurity by precipitation, gradient centrifugation, obtained protein of the methods of saltouing, these impurity are removed, simultaneously
The biological activity for keeping protein again, such as the catalytic activity of enzyme, it is necessary to be made accordingly according to different protein
Strategy, using different methods.Electrophoresis and chromatography are more commonly used methods, especially chromatography.
Liquid-solid chromatography is a kind of isolation technics based on absorption and dissolution properties, and column chromatography belongs to liquid-solid adsorption chromatography.
Since adsorbent is different to the adsorption capacity of each component, when mobile phase is by fixed phase surface, mixture each component is liquid-solid
Two accelerated trips.It is few in flowing phase partitioning to adsorb firm component, it is more in flowing phase partitioning to adsorb weak component.Mobile phase is passed through
When each component can be moved down with different rates, adsorb weak component and moved down with faster rate.With mobile phase
It is mobile, new distribution is carried out according to this absorption-course of dissolution again on the fixation phase surface newly contacted, fresh flow mutually flows through
This process is also repeated when the fixation phase surface for flattening out weighing apparatus, the result is that adsorbing weak component with phase shift is flowed in front, is inhaled
Later, the especially strong component of absorption even can not be with flowing phase shift, and various compounds are in chromatographic column for attached strong component movement
Middle formation zonal distribution, realizes the separation of mixture.
Column chromatography is a kind of isolation technics being in daily use, according to the property of each chemical composition in protein mixture
Difference selects different adsorbent and chromatographic solution, is gradually eluted to various compositions in mixture, collects eluent, reaches
The purpose of separation.The various compositions being dissolved in mobile phase are solvent concentration or are evaporated, and can obtain institute's extraction to be separated
Composition.
Because chromatography is more mild to the processing of protein, and the protein purification of biological activity can be largely prepared,
Therefore it is presently the most widely applied technical method.
Invention content
The purpose of this utility model is in view of the problems of the existing technology, to provide a kind of Separation of Proteins extraction element,
The various protein components in protein mixture can be separated and extracted on the basis of not destroying proteins biological activity.
To achieve the above object, the technical solution adopted in the utility model is：
A kind of Separation of Proteins extraction element is made of three batch can, liquid collecting tank and chromatographic column parts, and the batch can includes
Sequentially connected first batch can, the second batch can and third batch can；The liquid collecting tank includes the first liquid collecting tank, the second liquid collecting tank, the
Three liquid collecting tanks, the 4th liquid collecting tank；The chromatographic column includes the first chromatographic column and the second chromatographic column；Wherein first batch can goes out
Liquid mouth is connected with second batch can by the first catheter, and the waste liquid port of first batch can passes through with first liquid collecting tank
Third catheter is connected；The dialysis that second batch can is divided into upper storage room and lower storage room is equipped in second batch can
Film, the upper storage room are connected by the second catheter with the third batch can, the lower storage room by the 4th catheter with
Second liquid collecting tank is connected；It is equipped in the third batch can and the third batch can is divided into upper protein storage room and lower albumen
The molecular sieve of matter storage room, the upper protein storage room are connected by the 5th catheter with second chromatography column top, institute
It states the second chromatography column bottom with the 4th liquid collecting tank to be connected, the lower protein storage room bottom opening is inserted into the first layer
Column top is analysed, first chromatography column bottom is connected with the third liquid collecting tank.
It preferably, can mistake when mixed liquor flows to the first catheter equipped with strainer at the liquid outlet of first batch can
Filter the impurity in mixture.
Preferably, peristaltic pump is equipped between the upper storage room and the third batch can.When mixture mobility is poor, stream
When speed is slower, the flowing of mixed liquor can be accelerated by additional peristaltic pump.
Preferably, first chromatographic column and/or the second chromatography column top are circumscribed with peristaltic pump.The protein that need to be chromatographed point
Sub- mobility is poor, the slower protein molecule of flow velocity in chromatographic column, then needs additional peristaltic pump to improve the stream of mobile phase
Speed.
Further, the third liquid collecting tank and/or the 4th liquid collecting tank are rotating receiver.Traditional liquid collecting tank is being filled with
It after liquid, needs manually to operate to replace liquid collecting tank, operation when is often careful dismounting instrument, not so easily causes
The damage of instrument.Using rotating receiver, implementation automatically controls, and instead of hand labour, improves conventional efficient.The rotation
Receiver is made of multiple liquid storage bottles and turntable, and the time that the liquid storage bottle fills is set as the time of the turntable rotation
Interval realizes automation mechanized operation, saves manual labor.
Further, first batch can is connected with an agitating device.Mixed liquor is set to be uniformly mixed, the agitating device
Can be mechanical stirring device or magnetic stirring apparatus.
Compared with prior art, the utility model has the beneficial effects that：
Entire Separation of Proteins extraction process can be kept more efficiently and energy saving while keeping proteins biological activity,
In addition, during entire Separation of Proteins extraction, without adding any chemical reagents, easy to operate, mild condition.In conjunction with film
The advantages of isolation technics and chromatographic separation technology, being applied to Separation of Proteins extraction had unique advantage in one.
Description of the drawings
Fig. 1 is the structural schematic diagram of the utility model Separation of Proteins extraction element；
Fig. 2 is the structural schematic diagram of the utility model rotating receiver；
In figure：101, the first batch can；102, the second batch can；103, third batch can；201, the first liquid collecting tank；202, the second collection
Flow container；203, third liquid collecting tank；204, the 4th liquid collecting tank；301, the first catheter；302, the second catheter；303, third drain
Pipe；304, the 4th catheter；305, the 5th catheter；401, the first chromatographic column；402, the second chromatographic column；501, first piston
Valve；502, second piston valve；503, third piston valve；504, the 4th piston valve；505, the 5th piston valve；506, the 6th piston
Valve；507, the 7th piston valve；508, the 8th piston valve；6, strainer；7, dialysis membrane；8, molecular sieve.
Specific implementation mode
Below in conjunction with the attached drawing in the utility model, the technical solution of the utility model is clearly and completely retouched
It states, it is clear that the described embodiments are only a part of the embodiments of the utility model, instead of all the embodiments.Based on this
Embodiment in utility model, those of ordinary skill in the art obtained under the conditions of not making creative work it is all its
Its embodiment, shall fall within the protection scope of the present invention.
A kind of Separation of Proteins extraction element is made of three batch can, liquid collecting tank and chromatographic column parts, and the batch can includes
Sequentially connected first batch can 101, the second batch can 102 and third batch can 103；The liquid collecting tank includes the first liquid collecting tank 201, the
Two liquid collecting tanks 202, third liquid collecting tank 203, the 4th liquid collecting tank 204；The chromatographic column is chromatographed including the first chromatographic column 401 and second
Column 402；The liquid outlet of wherein first batch can 101 is connected with second batch can 102 by the first catheter 301, described
The waste liquid port of first batch can 101 is connected with first liquid collecting tank 201 by third catheter 303；In second batch can 102
Equipped with dividing second batch can 102 for the dialysis membrane 7 of upper storage room and lower storage room, the upper storage room passes through the second drain
Pipe 302 is connected with the third batch can 103, and the lower storage room passes through the 4th catheter 304 and 202 phase of the second liquid collecting tank
Even；It is equipped in the third batch can 103 and divides the third batch can 103 for upper protein storage room and lower protein storage room
Molecular sieve 8, the upper protein storage room are connected by the 5th catheter 305 and the top of second chromatographic column 402, and described the
Two chromatographic columns, 402 bottom is connected with the 4th liquid collecting tank 204, and the lower protein storage room bottom opening is inserted into described first
401 top of chromatographic column, 401 bottom of the first chromatographic column is connected with the third liquid collecting tank 203.
When it is implemented, the first batch can 101 stirs evenly mixed liquor under the action of agitating device, first piston is opened
Valve 501, mixed liquor flow to the second batch can 102 via the first catheter 301, cannot be left on the first material by the impurity of strainer 6
101 bottom of tank；First piston valve 501 is closed, the 4th piston valve 504, and the injected clear water in the first batch can 101, impurity are opened
Waste liquid is collected into through third catheter 303 in the first liquid collecting tank 201.
Mixed liquor in second batch can 102, under the action of dialysis membrane 7, small-molecule substance is collected in lower storage room, egg
White matter mixed liquor is collected into storage room；The 5th piston valve 505 is opened, small-molecule substance is collected through the 4th catheter 304
Into the second liquid collecting tank 202；Second piston valve 502 is opened, protein mixed liquor flows to third batch can through the second catheter 302
In 103.
Protein mixed liquor in third batch can 103, under the action of molecular sieve 8, small protein is collected in down
Protein storage room, macro-molecular protein are collected in protein storage room；Open the 6th piston valve 506, small molecular protein
Mass flow to the first chromatographic column 401, small protein because in chromatographic column flowing velocity it is different due to by different types of protein
It separates, opens the 7th piston valve 507 and collect different types of small protein in third liquid collecting tank 203；Open the
Three piston valves 503, macro-molecular protein flow to the second chromatographic column 402 through the 5th catheter 305, and macro-molecular protein is because chromatographing
Flowing velocity is different and open different types of Separation of Proteins in column, opens the 8th piston valve 508 and different types of will greatly divide
Sub- protein is collected in the 4th liquid collecting tank 204.
When it is implemented, the agitating device described in above example can be mechanical agitation, can also be magnetic agitation.
The agitating device can heat the material in first batch can 101 with mating heating device.
When it is implemented, in above example, the wriggling that is arranged between storage room and the third batch can 103 in opening
External peristaltic pump, accelerates protein mobile phase at the top of pump, first chromatographic column 401 and/or second chromatographic column 402
Flow velocity.
When it is implemented, in above example, the third liquid collecting tank 203 and/or the 4th liquid collecting tank 204 are rotation
Receiver, automation control collect liquid, reduce manual operation, more efficiently safer.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is appreciated that can these embodiments be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaiied
Change, replace and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.
Claims (6)
1. a kind of Separation of Proteins extraction element is made of, which is characterized in that institute three batch can, liquid collecting tank and chromatographic column parts
It includes sequentially connected first batch can, the second batch can and third batch can to state batch can；The liquid collecting tank includes the first liquid collecting tank, second
Liquid collecting tank, third liquid collecting tank, the 4th liquid collecting tank；The chromatographic column includes the first chromatographic column and the second chromatographic column；Wherein described
The liquid outlet of one batch can is connected with second batch can by the first catheter, the waste liquid port of first batch can and described first
Liquid collecting tank is connected by third catheter；It is equipped in second batch can and second batch can is divided into upper storage room and lower storage
The dialysis membrane of room, the upper storage room are connected by the second catheter with the third batch can, and the lower storage room passes through the 4th
Catheter is connected with second liquid collecting tank；It is equipped in the third batch can and the third batch can is divided into upper protein storage room
With the molecular sieve of lower protein storage room, the upper protein storage room chromatographs column top by the 5th catheter and described second
It is connected, second chromatography column bottom is connected with the 4th liquid collecting tank, and the lower protein storage room bottom opening is inserted into institute
The first chromatography column top is stated, first chromatography column bottom is connected with the third liquid collecting tank.
2. Separation of Proteins extraction element as described in claim 1, which is characterized in that set at the liquid outlet of first batch can
There is strainer.
3. Separation of Proteins extraction element as described in claim 1, which is characterized in that the upper storage room and the third material
Peristaltic pump is equipped between tank.
4. Separation of Proteins extraction element as described in claim 1, which is characterized in that first chromatographic column and/or second
It is circumscribed with peristaltic pump at the top of chromatographic column.
5. Separation of Proteins extraction element as described in claim 1, which is characterized in that the third liquid collecting tank and/or the 4th
Liquid collecting tank is rotating receiver.
6. Separation of Proteins extraction element as claimed in claim 2, which is characterized in that first batch can and an agitating device
It is connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721861787.6U CN207958198U (en) | 2017-12-27 | 2017-12-27 | Separation of Proteins extraction element |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721861787.6U CN207958198U (en) | 2017-12-27 | 2017-12-27 | Separation of Proteins extraction element |
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| Publication Number | Publication Date |
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| CN207958198U true CN207958198U (en) | 2018-10-12 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109970840A (en) * | 2019-02-26 | 2019-07-05 | 浙江海洋大学 | New type ultrasonic extracts reactor tank |
| CN111574584A (en) * | 2020-05-21 | 2020-08-25 | 中国科学院生物物理研究所 | Full-automatic protein purification system device and application thereof |
-
2017
- 2017-12-27 CN CN201721861787.6U patent/CN207958198U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109970840A (en) * | 2019-02-26 | 2019-07-05 | 浙江海洋大学 | New type ultrasonic extracts reactor tank |
| CN111574584A (en) * | 2020-05-21 | 2020-08-25 | 中国科学院生物物理研究所 | Full-automatic protein purification system device and application thereof |
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