CN209584204U - A kind of use for laboratory nucleic acid extracting instrument - Google Patents
A kind of use for laboratory nucleic acid extracting instrument Download PDFInfo
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- CN209584204U CN209584204U CN201821769808.6U CN201821769808U CN209584204U CN 209584204 U CN209584204 U CN 209584204U CN 201821769808 U CN201821769808 U CN 201821769808U CN 209584204 U CN209584204 U CN 209584204U
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- nucleic acid
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- filter
- accommodating chamber
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Abstract
The utility model discloses a kind of use for laboratory nucleic acid extracting instrument, including cracker, washing device, filter device, the filter that the filter device includes accommodating chamber, is arranged in the accommodating chamber, the filter include the frame being mutually clamped with the receiving cavity wall, several filter screens on said frame are mounted side by side.The utility model provides a kind of use for laboratory nucleic acid extracting instrument, and by adjusting the number of filter screen on said frame, the nucleic acid suitable for different content carries out purification operations；The nucleic acid on multiple filter screens can be washed and be desorbed simultaneously by the quantity of setting filter screen simultaneously, reduces the usage amount of reagent, cost is reduced, while simplifying the workload of operator, improves working efficiency.
Description
Technical field
The utility model belongs to molecular biosciences technical field, and in particular to a kind of use for laboratory nucleic acid extracting instrument.
Background technique
Nucleic acid is the carrier of hereditary information, is widely present in all animal and plant cells, in microbial body, is the most base of life
One of this substance pacifies the research and analysis of nucleic acid in clinical diagnosis, food with the fast development of Protocols in Molecular Biology
Entirely, the fields such as environment measuring, agricultural herding are constantly able to promotion and application.
It further include other substances such as protein, polysaccharide, therefore to study nucleic acid in biological cell in addition to nucleic acid
And analysis, need that extracting and developing comes out from complicated biotic environment by it.Therefore the extraction and purifying of nucleic acid are almost each
The all essential important process of Molecular Laboratory, main includes cracking, absorption, washing and desorption.
Chinese CN201710885874.3 patent is related to a kind of method for extracting nucleic acid and instrument is extracted in Bioexperiment, leads to
It crosses and cracking processing is carried out to sample, filter membrane adsorbs nucleic acid, and rinsing liquid cleaning impurity and eluent elute nucleic acid from filter membrane,
Pure nucleic acid solution is obtained, extracts nucleic acid in container, when carrying out elution nucleic acid, using overturning filter membrane, where changing nucleic acid
Position is quickly eluted.Realize the device of this method, main structure includes upper stack shell, lower stack shell, push rod and filter membrane
Plate is mainly characterized in that stack shell can be connected with each other with lower stack shell, and upper stack shell can be revolved relative to lower stack shell
Turn, and have the cavity of separated independent storage reagent in upper stack shell inner wall, has small hook on inner wall in lower stack shell, and have filter membrane
Plate, the utility model compact, is convenient for carrying, and easy to operate, is in suitable for average family and carries out nucleic acid extraction.It should
Device is cracked and is filtered using cylinder setting up and down, so that washing and desorption process are inconvenient, and not can be carried out a large amount of
The extraction of nucleic acid.
Chinese CN201721522538.4 patent is related to molecular biosciences technical field, and discloses a kind of experiment use
Nucleic acid extracting instrument, including pedestal, the surface of pedestal are fixedly installed with support plate, and the right side of support plate is equipped with purification process platform
With cracking operation platform, and cracking operation platform is located at the surface of purification process platform, and purification process platform and cracking operation platform open up
There is through-hole, the upper end of the through-hole of purification process platform is equipped with washing device, is equipped with cracker above the through-hole of cracking operation platform.It should
Experiment nucleic acid extracting instrument, by realizing liquid transition using gravity for cracking operation platform and the placement up and down of purification process platform,
It eliminates and frequently uses pipettor and replace the cumbersome of test tube, by setting washing device, the magnetic generated by energization solenoid
Magnetic bead is attached on adsorption plate, thus the liquid being convenient for changing in long-neck reaction flask and will not magnetic bead loss.The device
Paramagnetic particle method extraction purification nucleic acid is mainly used, more traditional paramagnetic particle method equipment therefor structure is more complicated, and reagent addition operation is difficult
It spends larger, is not suitable for the extraction and purifying of large quantities of nucleic acid samples.
Nucleic acid is being extracted in purification process, and each step requires different reagents and participates in reaction, traditional operating process
Middle operator needs frequent measurement reagent, replacement reaction vessel, and larger workload, the efficiency of operator is lower；It is existing
Nucleic acid-extracting apparatus cracking absorption is carried out to sample after, be only capable of that the nucleic acid of single adsorptions is washed and is desorbed, reagent makes
Dosage is larger, and number of operations is more, the larger workload of operator, more serious to the destruction of nucleic acid structure.Therefore, it is badly in need of setting
A kind of use for laboratory nucleic acid extracting instrument is counted to solve the above problems.
Utility model content
The purpose of this utility model is in view of the problems of the existing technology, to provide a kind of use for laboratory instrument for extracting nucleic acid
Device, by adjusting the number of filter screen on said frame, the nucleic acid suitable for different content carries out purification operations；Pass through simultaneously
The quantity that filter screen is arranged can wash and be desorbed simultaneously nucleic acid on multiple filter screens, reduce the usage amount of reagent, reduce at
This, while simplifying the workload of operator, improve working efficiency.
In order to achieve the above purposes, the technical solution adopted by the utility model is:
The utility model provides a kind of use for laboratory nucleic acid extracting instrument, including cracker, washing device, filtering dress
It sets, the filter device includes accommodating chamber, the filter that is arranged in the accommodating chamber, and the filter includes and the receiving
Several filter screens on said frame are mounted side by side in frame that cavity wall is mutually clamped, and the accommodating chamber and the filter screen are flat
Capable one side wall is equipped with the first water inlet, the first water outlet, and another parallel side wall is equipped with the second water inlet, the second water outlet
Mouthful, the cracker include the first cylinder, be arranged in that first cylinder body bottom matches with first water inlet the
Three water outlets are arranged in first intracorporal first piston, for driving the first piston in first cylinder
The first push rod to move reciprocatingly；The washing device includes the second cylinder, setting in second cylinder body bottom and described the
The 4th water outlet that two water inlets match, setting are in second intracorporal second piston, described second living for driving
Fill in the second push rod to move reciprocatingly in the one or two cylinder.
The working principle of a kind of use for laboratory nucleic acid extracting instrument described in the utility model are as follows: taken out using the cracker
It inhales lysate to be reacted with sample to be processed in first cylinder, after the reaction was completed, the cracker is mounted on
So that the third water outlet is connected on the filter device with the first water inlet, close the first water outlet and the second water inlet,
The first intracorporal solution is squeezed by first piston to enter in filter device, and solution is filtered by filter screen,
Several filter screens can be set on said frame according to the amount of sample to be processed；The utility model can be set several described
Cracker carries out cracking reaction simultaneously, after the completion of the solution filtering in a cracker, replaces another cracker and carries out
Filtering, by the way that the quantity of filter screen is arranged, can the larger amount of sample of disposable filtering, and can be improved by multistage filtering pure
Change efficiency, reduces nucleic acid and be lost, the lysate after absorption is discharged from second water outlet；After the completion of filtering, split by described
It solves device and aspirates cleaning solution, multiple filtration net is washed simultaneously by the first water inlet, waste liquid is from second water outlet
Discharge；After the completion of washing, eluent is aspirated in second cylinder by the washing device, the washing device is installed
So that the 4th water outlet is connected on the filter device with the second water inlet, close the first water inlet and the second water outlet, leads to
It crosses second piston and squeezes the second intracorporal liquid and enter in filter device and the nucleic acid adhered on filter screen is eluted,
Eluent is collected by the discharge of the first water outlet, and present apparatus structure can once elute multiple filter screens, is saved
Reagent, elution efficiency are high.
Suction unit is respectively equipped on first water outlet and second mouth of a river.It can make solution by the suction unit
Preferably by filter screen, it is discharged from the filter device；In addition, after completing nucleic acid extraction work, the suction unit
Convenient for being washed and being dried to filter device.
First water inlet and the second water inlet are equipped with seal washer.
It is connected with sample collection box at the first outlet, waste collection box is connected at the second outlet.
The opposite two sidewalls of the accommodating chamber are equipped with sliding slot, set on the interior side wall adjacent with the sliding slot of the accommodating chamber
There is the opening for being inserted into the frame；The frame is equipped with the sliding block matched with the sliding slot, the frame and opening
On be respectively equipped with mutually matched sealing structure.
The filter screen is detachably connected with the frame.
Graduation mark is equipped on first cylinder and the second cylinder.
Compared with prior art, the utility model has the beneficial effects that
(1) it after the utility model is cracked by the cracker, is filtered by filter device, by splitting
Solution device suction cleaning solution washs filter device, compared to traditional extracting mode, reduces the usage amount of reaction vessel, makes
The operation is more convenient；
(2) quantity of the filter screen is rationally arranged according to the amount of sample to be processed for the utility model, being capable of disposable mistake
A fairly large number of lysate is filtered, while by the way that multiple filter screens are arranged side by side, being capable of forming the structure of multistage filtering, making lysate
Filter effect it is more preferable, reduce inhereditary material be lost；
(3) washing device described in the utility model elutes the inhereditary material adhered on filter screen, the stream of eluent
Dynamic direction and the flow direction of lysate from filter screen on the contrary, can preferably elute the inhereditary material on filter screen, behaviour
Make simply, elution effect is good；
(4) the utility model can disposably elute multiple filter screens simultaneously, reduce amount of reagent, reduce behaviour
Make the workload of personnel, improves elution efficiency.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of use for laboratory nucleic acid extracting instrument described in the utility model；
Fig. 2 is the structural schematic diagram of filter described in the utility model；
In figure: 1, cracker；2, washing device；3, filter device；4, accommodating chamber；5, filter；6, frame；7, it filters
Net；8, the first water inlet；9, the first water outlet；10, the second water inlet；11, the second water outlet；12, the first cylinder；13, first
Piston；14, the first push rod；15, the second cylinder；16, second piston；17, the second push rod；18, suction unit；19, sample collection
Box；20, waste collection box；21, sealing structure；22, sliding block.
Specific embodiment
The technical solution of the utility model is clearly and completely described below, it is clear that described embodiment is only
The utility model a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's all other embodiment obtained under the conditions of not making creative work belongs to the utility model protection
Range.
Embodiment 1
A kind of use for laboratory nucleic acid extracting instrument of the present embodiment, including cracker 1, washing device 2, filter device 3, institute
Stating filter device 3 includes accommodating chamber 4, the filter 5 being arranged in the accommodating chamber 4, and the filter 5 includes and the receiving
Frame 6 that 4 inner wall of chamber is mutually clamped, several filter screens 7 being mounted side by side on the frame 6, the accommodating chamber 4 and the filtering
The parallel one side wall of net 7 be equipped with the first water inlet 8, the first water outlet 9, another parallel side wall be equipped with the second water inlet 10,
Second water outlet 11, first water inlet 8, the second water inlet 10, are equipped with list on the second water outlet 11 at first water outlet 9
To valve；The cracker 1 includes the first cylinder 12, is arranged in 12 bottom of the first cylinder and 8 phase of the first water inlet
The third water outlet of cooperation, the first piston 13 being arranged in first cylinder 12, for driving the first piston 13 to exist
The first push rod 14 to move reciprocatingly in first cylinder 12；The washing device 2 includes the second cylinder 15, is arranged described
The 4th water outlet that second cylinder, 15 bottom is matched with second water inlet 10, be arranged in second cylinder 15
Two pistons 16, the second push rod 17 for driving the second piston 16 to move reciprocatingly in second cylinder 15.
Seal washer is equipped at first water inlet 8 and the second water inlet 10.
It is connected with sample collection box 19 at the first outlet, waste collection box 20 is connected at the second outlet.
The opposite two sidewalls of the accommodating chamber 4 are equipped with sliding slot, in the accommodating chamber 4 on the side wall adjacent with the sliding slot
Equipped with the opening for being inserted into the frame 6；The frame 6 is equipped with the sliding block 22 matched with the sliding slot, the frame 6
With mutually matched sealing structure 21 is respectively equipped in opening.
The filter screen 7 is detachably connected with the frame 6.
Graduation mark is equipped on first cylinder 12 and the second cylinder 15.
First water inlet 8, the first water outlet 9, is respectively equipped with valve at the second water outlet 11 at second water inlet 10.
Two filter screens 7 are mounted side by side on the frame 6, for the less solution of inhereditary material concentration after cracking have compared with
Good filter effect, two layers of filter screen 7 are filtered step by step, improve the filter effect of inhereditary material, inhereditary material is prevented to be lost,
Influence experimental result；In addition, multiple filter screens 7 can be mounted side by side on the frame 6, to the biggish solution of inhereditary material concentration
It is handled, when rate of filtration decline, will be close to the filter screen 7 at the first water outlet 9 and remove, repetitive operation, until filtering
It completes, when elution, all filter screens 7 is mounted on the frame 6 and are eluted, the workload of operator is reduced, improve
Elution efficiency；It the utility model filtering direction and elutes contrary, is conducive to inhereditary material on filter screen 7 preferably and mistake
Strainer 7 is desorbed.When carrying out adsorption operations, the first water outlet 9 and the second water inlet 10 are closed, the cracker 1 can be erected
It is straight to place, so that the filter device 3 is placed in 1 lower section of cracker, is discharged convenient for eluent；Elution behaviour is carried out after the completion of filtering
Make, close the first water inlet 8 and the second water outlet 11 at this time, the washing device 2 is placed vertically, is placed in filter device 3
2 lower section of washing device, is collected convenient for eluent discharge.
Embodiment 2
A kind of use for laboratory nucleic acid extracting instrument of the present embodiment, compared with Example 1, the difference is that, described first
Suction unit 18 is respectively equipped on water outlet 9 and second mouth of a river.
The lysate more for inhereditary material concentration, filter device 3 can disposably handle lysate, be not necessarily to
Ceaselessly replace filter device 3；In the case where the rate of filtration of filter device 3 is reduced using at second water outlet 11
Suction unit 18 aspirates filter device 3, prevents filter screen 7 from blocking, and improves the rate of filtration.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired
Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.
Claims (7)
1. a kind of use for laboratory nucleic acid extracting instrument, which is characterized in that including cracker (1), washing device (2), filtering dress
It sets (3), the filter device (3) includes the filter (5) of accommodating chamber (4), setting in the accommodating chamber (4), the filtering
Device (5) includes the frame (6) being mutually clamped with the accommodating chamber (4) inner wall, several filterings being mounted side by side on the frame (6)
Net (7), the accommodating chamber (4) one side wall parallel with the filter screen (7) are equipped with the first water inlet (8), the first water outlet
(9), another parallel side wall is equipped with the second water inlet (10), the second water outlet (11)；The cracker (1) includes first
Third water outlet that body (12), setting are matched with first water inlet (8) in the first cylinder (12) bottom is arranged and exists
First piston (13) in first cylinder (12), for driving the first piston (13) in first cylinder (12)
The first push rod (14) to move reciprocatingly；The washing device (2) includes the second cylinder (15), is arranged in second cylinder
(15) bottom is matched with second water inlet (10) the 4th water outlet, second be arranged in second cylinder (15)
Piston (16), the second push rod for driving the second piston (16) to move reciprocatingly in second cylinder (15)
(17)。
2. a kind of use for laboratory nucleic acid extracting instrument according to claim 1, which is characterized in that first water outlet
(9) and on second mouth of a river suction unit (18) are respectively equipped with.
3. a kind of use for laboratory nucleic acid extracting instrument according to claim 1, which is characterized in that first water inlet
(8) and at the second water inlet (10) it is equipped with seal washer.
4. a kind of use for laboratory nucleic acid extracting instrument according to claim 1, which is characterized in that connect at the first outlet
It is connected to sample collection box (19), waste collection box (20) is connected at the second outlet.
5. a kind of use for laboratory nucleic acid extracting instrument according to claim 1, which is characterized in that accommodating chamber (4) phase
Pair two sidewalls be equipped with sliding slot, the side wall adjacent with the sliding slot is equipped with for being inserted into the frame in the accommodating chamber (4)
The opening of frame (6)；The frame (6) is equipped with the sliding block (22) matched with the sliding slot, divides in the frame (6) and opening
It She You not mutually matched sealing structure (21).
6. a kind of use for laboratory nucleic acid extracting instrument according to claim 1, which is characterized in that the filter screen (7) with
The frame (6) is detachably connected.
7. a kind of use for laboratory nucleic acid extracting instrument according to claim 1, which is characterized in that first cylinder (12)
Graduation mark is equipped with on the second cylinder (15).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821769808.6U CN209584204U (en) | 2018-10-30 | 2018-10-30 | A kind of use for laboratory nucleic acid extracting instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821769808.6U CN209584204U (en) | 2018-10-30 | 2018-10-30 | A kind of use for laboratory nucleic acid extracting instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209584204U true CN209584204U (en) | 2019-11-05 |
Family
ID=68375970
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821769808.6U Expired - Fee Related CN209584204U (en) | 2018-10-30 | 2018-10-30 | A kind of use for laboratory nucleic acid extracting instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209584204U (en) |
-
2018
- 2018-10-30 CN CN201821769808.6U patent/CN209584204U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191105 Termination date: 20201030 |