CN208171864U - Semiautomatic plant and detection device based on Solid Phase Extraction - Google Patents
Semiautomatic plant and detection device based on Solid Phase Extraction Download PDFInfo
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- CN208171864U CN208171864U CN201820465861.0U CN201820465861U CN208171864U CN 208171864 U CN208171864 U CN 208171864U CN 201820465861 U CN201820465861 U CN 201820465861U CN 208171864 U CN208171864 U CN 208171864U
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Abstract
The utility model relates to a kind of semiautomatic plants based on Solid Phase Extraction comprising：Sample inlets；Colvent inlet；Solid phase extraction filler section；Waste port；Fluid circuit, fluid circuit be constructed so that colvent inlet respectively with solid phase extraction filler section and with waste port selective fluid communication, make sample inlets respectively with solid phase extraction filler section and with waste port selective fluid communication and solid phase extraction filler section and waste port selective fluid communication can be made；Fluid circuit includes the first dosing channel, it includes predetermined first quantitative volume of energy, and at least one of the sample solution from sample inlets and the solvent from colvent inlet can flow into solid phase extraction filler section by the first dosing channel with the first quantitative volume.The utility model further includes a kind of detection device with aforementioned semiautomatic plant.The various solvents of Solid Phase Extraction can be fast and accurately quantified by this semiautomatic plant and detection device and thus improve test efficiency.
Description
Technical field
The utility model relates to a kind of semiautomatic plant based on Solid Phase Extraction, which is used for from sample solution
In Solid Phase Extraction is carried out to analyte, and further relate to comprising the detection device including this semiautomatic plant.
Background technique
In the detection and analysis work of chemical example, especially nature sample, the most common problem are that the component of sample is big
It is all excessively complicated when more, it can not directly be analyzed by chromatography, or directly analytical effect is bad.Therefore, it is necessary to by pair
Chemical example carries out pre-treatment to realize the separation of determinand, then carries out chromatography.
Most common chemical example pretreatment technology is Solid Phase Extraction (SPE) technology, i.e., by specific in component
The affinity of ingredient and solid extracting agent realizes sorption extraction, to realize the separation of special component.In essence, solid phase
Extraction is a kind of beneficiation technologies, and for sample by the extraction column of filling adsorbent, target analytes and impurity are retained in main column
On, impurity then is removed with selective solvent respectively, analyte is eluted, to achieve the purpose that separation.With liquid-liquid extraction phase
Than because its rate of recovery is good, the advantages such as operation is relatively easy, and solvent consumption is less, its application is becoming increasingly popular.
Conventional solid-phase extraction device is usually made of solid phase extraction column and auxiliary, is often related to column tube, sintering pad, is filled out
Numerous components such as material, vacuum system, vacuum pump, blow-dry device, inert gas source, high volume sampler and surge flask, it is difficult to realize
Micromation and integrated, and is not suitable for the processing of micro-example, to limit the automation of entire analytic process and portable
Property.
The Solid Phase Extraction of manual operation has pillar and disc type, uses vacuum plant or positive pressure devices mostly, and pillar is general
Multiple samples can be handled simultaneously, disc type is generally single processing.But manually solid phase extraction techniques also have its deficiency.It is right
In negative pressure device, the consistency of each channel treatment process it is difficult to ensure that, because each channel is in same negative pressure, influence each other.
For positive pressure devices, the cumbersome time-consuming of manual work, and may still have human error between the treatment process of every batch of sample.
In particular, laboratory staff needs to carry out multi-solvents to take liquid in the purification process based on Solid Phase Extraction.Mesh
Before, it generallys use pipettor and manually solvent is applied on solid-phase extraction column.It will be understood that the amount and ratio of various solvents
Example is very crucial.Although taking liquid to may insure that the amount of various solvents is in the main true manually currently with pipettor,
But it operates and expends very much energy, this is very inefficient for the laboratory of every step is repeated and works.
In addition, the current device based on Solid Phase Extraction is generally all placed in laboratory, testing crew needs will be wait divide
The sample of analysis gets back to laboratory, then carry out again Solid Phase Extraction, with analyzer detect etc., whole process is very time-consuming, unfavorable
In effective report of analysis result, this is dangerous for certain situations.
So far, in Solid Phase Extraction field also always exist to fast and accurately quantify Solid Phase Extraction various solvents and by
This improves the demand of test efficiency.
Utility model content
The utility model provides a kind of semiautomatic plant based on Solid Phase Extraction, which is used for from sample solution
In to analyte carry out Solid Phase Extraction, and including：Sample inlets, the sample solution enter the semi-automatic dress via the sample inlets
It sets；The various solvents of colvent inlet, Solid Phase Extraction enter the semiautomatic plant via the colvent inlet；Solid phase extraction filler section,
The solvent and the sample solution can flow separately through the solid phase extraction filler section, to obtain the analyte through Solid Phase Extraction；
Waste port, extra solvent and sample solution can leave the semiautomatic plant via the waste port；Wherein, described semi-automatic
Device further includes：Fluid circuit, the fluid circuit be constructed so that the colvent inlet respectively with the solid phase extraction filler
Section and with the waste port selective fluid communication, make the sample inlets respectively with the solid phase extraction filler section and with institute
It states waste port selective fluid communication and the solid phase extraction filler section and the waste port selectively fluid company can be made
It is logical；Wherein, fluid circuit includes the first dosing channel, and first dosing channel includes can the predetermined first quantitative appearance
Product, at least one of the sample solution from the sample inlets and the solvent from the colvent inlet can lead to
It crosses first dosing channel and the solid phase extraction filler section is flowed into first quantitative volume.
By above-mentioned semiautomatic plant, may be implemented quickly and accurately quantitatively for the various solvents of Solid Phase Extraction and examination
Sample greatly improves test efficiency, realizes Solid Phase Extraction to mitigate the workload that testing crew measures solvent and sample repeatedly
It is semi-automatic.
Especially, sample solution and the solvent can separately flow into first dosing channel, and with described first
Quantitative volume is held in first dosing channel, and the excessive sample solution and the solvent flow to the waste material
Mouthful, the sample solution and the solvent being thus held in first dosing channel again respectively can be quantitative with described first
Volume flows to the solid phase extraction filler section.
Due to solvent and sample solution are maintained in the first dosing channel to constant volume and made extra solvent and
Sample solution can flow into waste port, and the accurate quantification of solvent and sample solution may be implemented, and thus avoid inaccurately quantitative
Test error and the labor intensive generated for accurate quantification.
In particular, semiautomatic plant can also include that analyzing container exports, the fluid circuit is constructed so that described solid
Phase extraction stuffing section is connected to the analyzing container egress selection, so that the analyte through Solid Phase Extraction is via above-mentioned point
It analyses container outlet and flows out semiautomatic plant.The analyte through Solid Phase Extraction can export via analyzing container and leave this partly as a result,
Automatic device, subsequently into being ready for analyzing in analyzing container.
Preferably, fluid circuit can also include the second dosing channel, second dosing channel also includes can in advance really
The second fixed quantitative volume, wherein one of multi-solvents are eluting solvent, and the eluting solvent is with the described first quantitative appearance
Product flows into the solid phase extraction filler section, and the analyte eluted flows into described second from the solid phase extraction filler section and determines
It measures channel and the analyzing container outlet is flowed to second quantitative volume.
The analyte eluted as a result, also can very easily accurate quantification so that post analysis test result
More effectively.
In addition, the aggregates are via described poly- it is particularly favourable that the semiautomatic plant can also include aggregates entrance
The entrance that gathers materials flows into one of first dosing channel and second dosing channel, and with the corresponding first quantitative appearance
Long-pending or the second quantitative volume flows directly into the analyzing container outlet.By the aggregates of accurate quantification, so that at subsequent point
It analyses in container, the analysis that can be carried out to the analyte obtained from the sample can be related to Raman spectrum analysis method, especially may be used
It is related to the detection based on Surface enhanced Raman scattering.
Advantageously, the first dosing channel may include entrance and exit, it is equipped in the entrance and the exit
Valve, respectively to select the sample solution for flowing through first dosing channel and the solvent with first quantitative volume
It is held in selecting property in first dosing channel.By valve of this set at the first dosing channel both ends, Ke Yiyou
Effect ground control solvent and sample solution are maintained in first dosing channel, and open valve when needed, to make accurate
Quantitative solvent and sample solution flows to analyzing container outlet.
In particular, the semiautomatic plant can be also configured to portable solid-phase extraction chip, wherein the sample inlets,
Each colvent inlet, the waste port, the solid phase extraction filler section, analyzing container outlet and the fluid circuit collect
At on the solid-phase extraction chip, and the selectivity fluid communication of the fluid circuit can be controlled by microprocessor.Institute as a result,
Some ports integrate on the same chip, so that can be achieved with the whole process of Solid Phase Extraction by chip piece, and real
Existing precision test.
Preferably, various solvents energy the first dosing channel of respective multipass, thus adds up with the whole of the first quantitative volume
The volume of several times flows into the solid phase extraction filler section.It can be realized as a result, by the first dosing channel of fixed volume various
Different required volumes quantifies so that component is compact, but the scope of application but very flexibly.
The utility model additionally provides a kind of detection device, which includes semiautomatic plant as described above, more
A solvent storage containers, sample preparation device, analyzing container and the analysis instrument for being analyzed the analyte,
In, the solvent storage containers are connected respectively to the colvent inlet of the semiautomatic plant, are used for Solid Phase Extraction to provide
Various solvents, the sample preparation device is connected to the sample inlets of the semiautomatic plant, is prepared with providing
Sample solution, and the analyzing container is connected to the analyzing container outlet of the semiautomatic plant, is extracted with receiving through solid phase
The analyte taken.
By above-mentioned detection device, testing crew can on the spot can be to from sample solution solid phase extraction obtain sample
Analyte afterwards carries out field assay, so that test efficiency is greatly improved, so that whole process is integrated, portability.
It especially, can also include metal nanoparticle in the analyzing container, so that the analyte eluted, aggregation
Material and the metal nanoparticle can mix in the analyzing container, can carry out surface-enhanced Raman to the analyte
Scattering analysis.Thereby, it is possible to obtain the analytical effect of the analyte after very good Solid Phase Extraction.
Detailed description of the invention
Fig. 1 shows the schematic diagram of each step of Solid Phase Extraction well known in the prior art；
Fig. 2 shows the schematic diagrames of the operating procedure of semiautomatic plant according to the present utility model, wherein pumps adsorption solvent
It is sent to the first dosing channel, extra adsorption solvent flows to waste port；
Fig. 3 shows the schematic diagram of the operating procedure of semiautomatic plant according to the present utility model, wherein various solvents and examination
Sample solution is successively delivered to solid phase extraction filler section with the first quantitative volume, and extra solvent or sample solution is then flowed to and given up
Material mouth；
Fig. 4 shows the schematic diagram of the operating procedure of semiautomatic plant according to the present utility model, wherein eluting solvent will try
Analyte in sample solution elutes；
Fig. 5 shows the schematic diagram of the operating procedure of semiautomatic plant according to the present utility model, wherein aggregates are quantitatively
It is added into analyzing container；And
Fig. 6 shows the schematic diagram of detection device according to the present utility model.
Specific embodiment
In the present invention, term " sample " refers to each substance detected, especially, examines in biology
Each substance that the fields such as survey, medical diagnosis on disease, environmental monitoring, chemical analysis are detected, including but is not limited to is chemistry
Sample, Biosample.
In particular, the analysis carried out to the analyte obtained from the sample can be related to Raman spectrum analysis method, especially
It is that can be related to the detection based on Surface enhanced Raman scattering.Raman spectrum includes the vibration and rotation information of molecule, thus can table
Up to molecular structure information abundant, " fingerprint characteristic " of molecule can be obtained by the Raman spectrum of substance.Identical molten
Under liquid experimental condition, Raman is easier to obtain than the fluorescence spectrum of wide spectrum, and for non-fluorescence substance, Raman light
Spectrum can not need to make fluorescent marker, and Raman spectrum can obtain in the non-vibration area of molecule, avoid fluorescence " bleaching "
Effect.
In order to carry out the Solid Phase Extraction as referred in background technique, sample solution is first usually prepared before operation herein, i.e.,
Any type of sample is converted to flowable solution form.Hereinafter, term " sample " and " sample solution " be can phase
It mutually replaces, unless otherwise indicated.
Semiautomatic plant based on Solid Phase Extraction, which discards tradition, to need to carry out taking liquid manually to the various solvents of Solid Phase Extraction
Operation, but a kind of significant semiautomatic plant of amount of labour for mitigating testing crew.Term " semi-automatic " refers to not with shifting
Liquid device is operated manually, but is also not the operation being fully automated, and can be significantly improved because being fully automated the cost of device.
In order to make it easy to understand, being briefly described first to conventional Solid Phase Extraction program：
In general, Solid Phase Extraction includes the portion in the solid phase extraction filler column for making sample solution by filling adsorbent
Divide or whole components are adsorbed on the solid phase extraction filler column, is then cleaned with one or more mixed solvents, make impurity
Be detached from solid phase extraction filler column, finally again use a small amount of rapid elution analysis object of solution, thus reach quick separating purification with it is dense
The purpose of contracting.
Specifically, as shown in fig. 1, in the first step (that is, regulating step, schemes referring to first from left to right), with tune
Section solvent, such as methanol break through solid phase extraction filler column, can also break through solid phase extraction again with other adjusting solvents, such as water again
Take filled column.
In second step (that is, loading step, schemes referring to second from left to right), sample is applied to solid phase extraction filler
Column, such as sample can be pushed through with a certain speed or with being pumped through solid phase extraction filler column.In this process, certain interfering substances
It will be flowed out from solid phase extraction filler column.
It, will with rinse solvent, such as polar solvent in third step (that is, rinsing step, schemes referring to third from left to right)
Most of chaff interferent rinses.
In four steps (that is, elution step, schemes referring to the 4th from left to right), a small amount of eluant, eluent, such as 1-2 milliliters are utilized
Nonpolar solvent required analyte is eluted, and collect these analytes to carry out next step analysis and research.
It can thus be seen that in entire solid phase extraction procedure, various solvents especially adjust solvent, rinse solvent, wash
Desolventizing can flow through solid phase extraction filler column.In addition, sample is retained by it also by the mode for flowing through solid phase extraction filler column
Get off.Thus, various solvents and the quantitative of sample are particularly important in test.
In this regard, semiautomatic plant 100 according to the present utility model include sample inlets 90, through preparing sample solution (or
Person itself has been the sample of solution form) semiautomatic plant 100 can be entered via the sample inlets 90.It is contemplated that should
Sample inlets 90 are connected with the sample solution storage container being located at except semiautomatic plant 100.
Meanwhile semiautomatic plant 100 further includes colvent inlet 40, for the various solvents of Solid Phase Extraction including but unlimited
The semiautomatic plant 100 can be entered via colvent inlet 40 in various solvents mentioned above.It is contemplated that the colvent inlet
40 be located at semiautomatic plant 100 except for accommodate the solvent storage device of various solvents, such as solvent cell is connected.
It is understood that one or more colvent inlets 40 can be within the protection scope of the utility model.It is particularly advantageous
, various solvents enter semiautomatic plant 100 via respective colvent inlet 40 respectively, such as can be by various valves, example
Realized such as operated pneumatic valve or machinery valve make various solvents successively or any given sequence via respective colvent inlet 40
Into semiautomatic plant 100.But it is also possible that various solvents enter via same colvent inlet 40.In the case, this is molten
The needs of agent entrance 40 dock the solvent storage device for accommodating various solvents, such as solvent cell via a variety of different pipelines.
In addition, the solid phase extraction filler section 80 needed for solid phase extraction procedure be also contained in it is according to the present utility model
In semiautomatic plant 100.The solid phase extraction filler section 80 for example can be solid phase extraction resin.
In addition, the semiautomatic plant 100 may also include waste port 10, as previously mentioned, in solid phase extraction procedure, it is various more
Remaining solvent, sample or the chaff interferent flushed out need to be discharged the semiautomatic plant 100 or be discharged into it is other accommodate this
In the collecting devices of a little substances, such as waste tank.
It is understood that need there are various fluid circuits to be attached between aforementioned each entrance or section,
Especially make to can be realized selective fluid communication between them.For example, fluid circuit is configured to make colvent inlet 40
Respectively with solid phase extraction filler section 80 and with 10 selective fluid communication of waste port, make sample inlets 90 respectively with Solid Phase Extraction
Packing section 80 and with 10 selective fluid communication of waste port and solid phase extraction filler section 80 and the selectivity of waste port 10 can be made
Ground is in fluid communication.But the utility model is not limited to the fluid circuit of above-mentioned various connections, but there may also be connect other portions
Fluid circuit between part.
In order to not carried out taking liquid by pipettor by testing crew, need to based on Solid Phase Extraction various solvents and
Sample is quantified.For this purpose, according to the utility model, at least provided with the first dosing channel 50 in the semiautomatic plant 100.
Particularly advantageously, which belongs to a part of the fluid circuit of semiautomatic plant 100 because when not need into
When row is quantitative, which can be used for the fluid communication between various pieces, thus reduce the semi-automatic dress
Set 100 volume.Certainly, the first dosing channel 50 is included in semiautomatic plant 100 independently of fluid circuit and also belongs to this
Within the protection scope of utility model.
First dosing channel 50 includes predetermined first quantitative volume, such as 0.5 milliliter.As shown in Figure 2, should
First dosing channel 50 can be arranged to the form for the pipeline that multistage connects from beginning to end each other, but the form of the first dosing channel 50 is not
It is limited to this, such as is also possible to one section of straight pipeline, as long as it has determining volume.
According to the utility model, in the sample solution from sample inlets 90 and the solvent from colvent inlet 40 at least
One, preferably the two can flow into solid phase extraction filler section 80 by first dosing channel 50 with the first quantitative volume.More
For body, sample solution and solvent are separately flowed into, for example with defined order the first dosing channel 50 of inflow, at this point, first is fixed
It is filled by corresponding sample solution or solvent in amount channel 50.Determine in general, the amount of the sample solution and solvent that flow into is greater than first
Volume is measured, therefore ensure that sample solution and solvent in first dosing channel 50 and any gap or bubble is not present.
Therefore, the sample solution and solvent of inflow can be held in the first dosing channel 50 with the first quantitative volume.For example,
First dosing channel 50 may include that entrance and exit, entrance and colvent inlet 40 and/or the selective fluid of sample inlets 90 connect
It is logical, and its outlet is then in fluid communication with 10 selectivity of waste port.When sample solution and solvent flow through in the first dosing channel 50,
The entrance and exit (for example, by closing the valve being located at entrance and exit) of the first dosing channel 50 can be closed, thus
Sample solution and solvent is set to be held in the first dosing channel 50 with the first quantitative volume.At this point, excessive or extra sample
Solution and solvent then flow to waste port 10 and the semiautomatic plant then are discharged via waste port 10 if necessary.Then, pass through
Be again turned on the first dosing channel 50 entrance and exit (outlet at this time is connect with solid phase extraction filler section 80, and no longer with
Waste port 10 connects), the sample solution and solvent being held in the first dosing channel 50 before just respectively can be first quantitative with this
Volume flows to solid phase extraction filler section 80, to be completed with very simple structure and easy operation to quantitative sample solution
With the acquisition of solvent.
In the following, illustratively illustrating each step of solid phase extraction manipulations according to the present utility model by Fig. 2-5：
As shown in Figure 2, adsorption solvent is pumped into the first dosing channel 50, extra adsorption solvent stream using vacuum source
To waste port 10.
Then, as shown in Figure 3, for the various solvents and sample solution of Solid Phase Extraction successively (for example, with above-mentioned adjusting
The sequence of solvent, sample solution, rinse solvent) via colvent inlet 40 (for solvent) or sample inlets 90 (for sample)
Into the semiautomatic plant 100, and it is quantitative via the first dosing channel 50, it is solid so as to be delivered to the first quantitative volume
Phase extraction stuffing section 80 (that is, resin column).Since solid phase extraction filler section 80 can be connected to waste port 10, thus, extra is molten
Agent or sample solution then flow to waste port 10.
It is different in view of the requirement to various solvents, therefore can repeatedly utilize this first quantitative for same solvent
Channel 50, so that a certain solvent repeatedly (that is, more times of the first quantitative volume) can enter Solid Phase Extraction with the first quantitative volume
Packing section 80.
It is envisaged that the first dosing channel 50 includes multiple first dosing channels 50 for being connected in parallel to each other, and these the
A certain amount of channel 50 includes outlet independent and inlet valve, and it is quantitative logical thus can to open multiple first for certain solvents
Road, flowing into solid phase extraction filler section 80 thus, it is possible to the volume of the integral multiple of the first quantitative volume of energy quickly, (integer is greater than
Zero).
As shown in Figure 4, another solvent, for example, eluting solvent are quantified by the first dosing channel, thus every time
Flow into solid phase extraction filler section 80 with the first quantitative volume (solvent of margin then flows to waste port 10).For example, working as the solvent
When for eluant, eluent, which flows through the first dosing channel 50 at least twice, thus with the total of twice first quantitative volume
Volume flows into solid phase extraction filler section 80.
Analyte can be eluted from sample solution as a result,.In order to which the analyte for obtaining quantitative is analyzed, according to this reality
It can also include the second dosing channel with novel semiautomatic plant 100, which is also similarly included can be in advance
Determining the second quantitative volume (fixed volume).The analyte eluted can flow directly into this from solid phase extraction filler section 80
Second dosing channel is to be quantified.
Similar with above with respect to the first dosing channel 50, the second dosing channel may include entrance and exit, entrance with
80 selectivity of solid phase extraction filler section is in fluid communication, and its outlet is then flowed with waste port 10 or 20 selectivity of analyzing container outlet
Body connection.When the analyte eluted flows through in the second dosing channel, the entrance and exit of the second dosing channel can be closed
(for example, by closing the valve being located at entrance and exit), thus enables the analyte eluted protect with the second quantitative volume
It is held in the second dosing channel.At this point, the excessive or extra analyte eluted then flows to waste port 10 and if necessary
The semiautomatic plant 100 then is discharged via waste port 10.Then, by being again turned on the entrance and exit of the second dosing channel
(outlet at this time is connect with analyzing container outlet 20, and is no longer connect with solid phase extraction filler section 80), is held in second before
Analyte in dosing channel can flow to analyzing container outlet 20 with second quantitative volume, thus with very simple structure
The acquisition to quantitative analyte is completed with easy operation.
Preferably, the first quantitative volume is different from the second quantitative volume, but can also be identical.
It is understood that the analyzing container outlet 20 be located at semiautomatic plant 100 except analyzing container, be, for example, extremely
Few analysis bottle connection, thus quantitative analyte can be admitted in the analyzing container.In the analyzing container, advantageously
Metal nanoparticle is had existed.
In additional step as shown in Figure 5, which can also include aggregates entrance 30, aggregation
Material flows into semiautomatic plant 100 via the aggregates entrance 30.Advantageously, the aggregates are also by semiautomatic plant 100
Fluid circuit quantitatively, for example determine via one of the first dosing channel 50 and second dosing channel, with corresponding first
Amount volume or the second quantitative volume flow directly into analyzing container outlet 20, hence into analyzing container connected to it.But it can be with
It is understood that can also except semiautomatic plant 100 directly by this aggregates be added it is existing have metal nanoparticle and wash
In the analyzing container of the analyte of abjection.
It is understood that aggregates are that can make the colloid aggregation to generate for enhancing Surface enhanced Raman scattering signal
Hot spot material.Therefore, aggregates belong to known chemical material, such as all kinds of salt.Thus, it is possible to very simple enhance
Surface enhanced Raman scattering signal, it is ensured that analyze the correctness of result.
Furtherly, as seen in figs. 2-5, semiautomatic plant 100 according to the present utility model is preferably configured as just
Take formula solid-phase extraction chip.Here, aforementioned specimen entrance 90, each colvent inlet 40, waste port 10, solid phase extraction filler section
80, analyzing container outlet 20 and fluid circuit can be advantageously integrated on the solid-phase extraction chip.In other words, basic operation
Unit has been can integrate or has been integrated into substantially now on one piece several square centimeters of chip, forms network by microchannel, to
Different biological or chemical reaction process is completed, and its product is analyzed.
In particular, the selectivity stream by fluid circuit between the various pieces of semiautomatic plant 100 detailed earlier herein
Body connection can be controlled by microprocessor.For example, it is integrated on the chip or microprocessor except the chip can be with
The various connections or cutting, such as control for controlling fluid circuit are located at the closing of the valve on each fluid circuit part and beat
It opens.In addition, the microprocessor also can control the sequence of flow of various solvents and sample solution etc., i.e., flowed through with set sequence solid
Phase extraction stuffing section 80 (for example, resin column).
In traditional solid phase extraction procedure, consumable injection is packaged in using the solid phase extraction filler that cartridge type designs
In the pipe of device shape.Testing crew must by suitable solvent correctly to measure imbibition into the cylinder, and allow gravity or
Slight underpressure source makes each solvent be pulled through the solid phase extraction filler cylinder.This usually require Solid Phase Extraction different phase respectively into
The various solvents of row or sample take liquid.
In contrast to this, solid phase extraction filler column is directly covered one by semiautomatic plant 100 according to the present utility model
On chip.The chip is manufactured at least one quantitative channel, multiple colvent inlets 40 and suitable valve, thus will be each molten
With them, correctly amount is sent to solid phase extraction filler section 80 for agent.The process becomes no longer completely manual as a result, and can be carried out quickly
Analysis, and the physical demands of testing crew is greatly reduced.
In addition, solvent or other materials for reaction can be previously integrated to the chip, for example, by using bubble-cap technology
To seal, and feeding solid phase extraction filler section 80 or analyzing container export 20 when needed.
Finally, the utility model further includes a kind of detection device.The detection device may include semi-automatic dress as described above
Set 100, multiple solvent storage containers 200, sample preparation device 400, analyzing container and for being analyzed analyte
Analysis instrument 300, as shown in Figure 6.
For example, solvent storage containers are connected respectively to each colvent inlet 40 of semiautomatic plant 100, to provide for solid
The mutually various solvents of extraction.Sample preparation device 400 is connected to the sample inlets 90 of semiautomatic plant 100, to provide through preparing
Sample solution (sample can form solution in the sample preparation device 400).Analyzing container may be coupled to semi-automatic dress
100 analyzing container outlet 20 is set, to receive the analyte through Solid Phase Extraction.
As previously mentioned, analyzing container can wrap containing metal nanoparticle so that the analyte eluted, aggregates and
The metal nanoparticle can mix in the analyzing container, can carry out Surface enhanced Raman scattering point to analyte
Analysis.
As shown in Figure 6, operation can be provided in the chest by the design of testing device at portable chest is convenient for
Panel.Advantageously, by aforementioned semiautomatic plant 100, multiple solvent storage containers, sample preparation device 400, analyzing container and
Analysis instrument 300 is respectively embedded among the operation panel.
In particular, the display or graphic user interface 320 of analyzer 300 can directly display on the operation panel
Out, to be conducive to each steps flow chart that testing crew controls Solid Phase Extraction and analysis.
In-site collecting sample may be implemented by such detection device, the analyte Solid Phase Extraction in sample is come out,
Then the various analyses such as spectrum analysis are carried out, to can be analyzed immediately as a result, to accelerate test efficiency.
The description of front has been presented for many features and advantage, including various alternative embodiments and device
The details of structure and function.It is intended that it is illustrative, it is not exhaustive or restrictive.
It for those skilled in the art obviously can be to the wide in range upper of the term as expressed by appended claims
Various remodeling are made in full scope indicated by meaning, especially in structure, material, element, component, shape, size and portion
In terms of the arrangement of part, including the combination in these aspects concept described herein.These it is various remodeling without departing from
In the degree of spirit and scope of the appended claims, it is meant that they are also incorporated herein.
Claims (10)
1. a kind of semiautomatic plant (100) based on Solid Phase Extraction, the semiautomatic plant (100) is for right from sample solution
Analyte carry out Solid Phase Extraction and including：
Sample inlets (90), the sample solution enter the semiautomatic plant via the sample inlets (90)；
The various solvents of colvent inlet (40), Solid Phase Extraction enter the semiautomatic plant via the colvent inlet (40)；
Solid phase extraction filler section (80), the solvent and the sample solution can flow separately through the solid phase extraction filler section
(80), to obtain the analyte through Solid Phase Extraction；
Waste port (10), extra solvent and sample solution can leave the semiautomatic plant via the waste port (10)；
It is characterized in that, the semiautomatic plant (100) further includes：
Fluid circuit, the fluid circuit be constructed so that the colvent inlet (40) respectively with the solid phase extraction filler section
(80) and with the waste port (10) selective fluid communication, fill out the sample inlets (90) with the Solid Phase Extraction respectively
Material section (80) and with the waste port (10) selective fluid communication and the solid phase extraction filler section (80) and institute can be made
State waste port (10) selective fluid communication；
Wherein, the fluid circuit includes the first dosing channel (50), and first dosing channel (50) includes that can predefine
The first quantitative volume, the sample solution from the sample inlets (90) and described from the colvent inlet (40)
At least one of solvent can flow into the Solid Phase Extraction by first dosing channel (50) with first quantitative volume
Packing section (80).
2. the semiautomatic plant based on Solid Phase Extraction (100) as described in claim 1, which is characterized in that the sample solution
First dosing channel (50) can be separately flowed into the solvent, and is held in described first with first quantitative volume
In dosing channel (50), and the excessive sample solution and the solvent flow to the waste port (10), are thus held in institute
It states the sample solution and the solvent in the first dosing channel (50) and institute can be flowed to respectively with first quantitative volume
State solid phase extraction filler section (80).
3. the semiautomatic plant based on Solid Phase Extraction (100) as claimed in claim 2, which is characterized in that further include that analysis is held
Device exports (20), and the fluid circuit is constructed so that the solid phase extraction filler section (80) and analyzing container outlet (20)
It selectively communicates with, so that the analyte through Solid Phase Extraction, which exports (20) via above-mentioned analyzing container, flows out the semi-automatic dress
It sets.
4. the semiautomatic plant based on Solid Phase Extraction (100) as claimed in claim 3, which is characterized in that the fluid circuit
Including the second dosing channel, second dosing channel includes predetermined second quantitative volume of energy, wherein in multi-solvents
One kind be eluting solvent, the eluting solvent flows into the solid phase extraction filler section (80) with first quantitative volume, and
And the analyte eluted flows into second dosing channel from the solid phase extraction filler section (80) and determines with described second
Amount volume flows to the analyzing container outlet (20).
5. the semiautomatic plant based on Solid Phase Extraction (100) as claimed in claim 4, which is characterized in that further include aggregates
Entrance (30), the aggregates, which flow into first dosing channel (50) and described second via the aggregates entrance (30), to be determined
One of channel is measured, and the analyzing container outlet is flowed directly into corresponding first quantitative volume or the second quantitative volume
(20)。
6. the semiautomatic plant based on Solid Phase Extraction (100) as claimed in claim 5, which is characterized in that described first is quantitative
Channel (50) includes an inlet and an outlet, and valve is equipped in the entrance and the exit, will flow through described first respectively
The sample solution and the solvent of dosing channel (50) are selectively held in described first with first quantitative volume
In dosing channel (50).
7. the semiautomatic plant based on Solid Phase Extraction (100) as claimed in claim 6, which is characterized in that the semi-automatic dress
It sets and is configured to portable solid-phase extraction chip, wherein the sample inlets (90), each colvent inlet (40), the waste port
(10), the solid phase extraction filler section (80), analyzing container outlet (20) and the fluid circuit are integrated in described solid
Mutually on extraction chip, and the selectivity fluid communication of the fluid circuit can be controlled by microprocessor.
8. the semiautomatic plant based on Solid Phase Extraction (100) as claimed in claim 7, which is characterized in that the various solvents
Thus respective the first dosing channel of multipass (50) of energy adds up to flow into the volume of the integral multiple of the first quantitative volume described solid
Phase extraction stuffing section (80).
9. a kind of detection device, the detection device include as of any of claims 1-8 based on Solid Phase Extraction
Semiautomatic plant (100), multiple solvent storage containers (200), sample preparation device (400), analyzing container and for institute
The analysis instrument (300) that analyte is analyzed is stated,
Wherein, the solvent storage containers are connected respectively to the colvent inlet (40) of the semiautomatic plant, to provide use
In the various solvents of Solid Phase Extraction, the sample preparation device is connected to the sample inlets (90) of the semiautomatic plant,
To provide the sample solution through preparing, and the analyzing container is connected to the analyzing container outlet (20) of the semiautomatic plant,
To receive the analyte through Solid Phase Extraction.
10. detection device as claimed in claim 9, which is characterized in that it include metal nanoparticle in the analyzing container,
So that the analyte eluted, aggregates and the metal nanoparticle can mix in the analyzing container, with can be right
The analyte carries out Surface enhanced Raman scattering analysis.
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| CN111443074A (en) * | 2020-05-11 | 2020-07-24 | 厦门大学 | Automatic on-line pretreatment and Raman detection device and method thereof |
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| CN111443074A (en) * | 2020-05-11 | 2020-07-24 | 厦门大学 | Automatic on-line pretreatment and Raman detection device and method thereof |
| CN111443074B (en) * | 2020-05-11 | 2021-08-31 | 厦门大学 | Automatic on-line pretreatment and Raman detection device and method thereof |
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