CN201133904Y - Negative-pressure liquid phase chromatograph - Google Patents

Negative-pressure liquid phase chromatograph Download PDF

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Publication number
CN201133904Y
CN201133904Y CNU2008200652170U CN200820065217U CN201133904Y CN 201133904 Y CN201133904 Y CN 201133904Y CN U2008200652170 U CNU2008200652170 U CN U2008200652170U CN 200820065217 U CN200820065217 U CN 200820065217U CN 201133904 Y CN201133904 Y CN 201133904Y
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CN
China
Prior art keywords
chromatographic column
negative pressure
liquid
negative
electronic control
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CNU2008200652170U
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Chinese (zh)
Inventor
朱海波
邓建平
徐继勋
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朱海波
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Publication date
Application filed by 朱海波 filed Critical 朱海波
Priority to CNU2008200652170U priority Critical patent/CN201133904Y/en
Application granted granted Critical
Publication of CN201133904Y publication Critical patent/CN201133904Y/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

The utility model relates to an automatic analyzing instrument, in particular to a negative-pressure, liquid-phase chromatographic instrument, which is provided with a plurality of reagent tanks, and the reagent tanks are orderly connected with electromagnetic valves, a mixer, an electromagnetic valve, a chromatographic column, an optical colorimetric system and a waste liquid tank and are provided with an electronic control system. The automatic analyzing instrument has the following characteristics: (a) a peristaltic pump is added on the front section of the chromatographic column; (b) the chromatographic column is of an open structure, and a liquid level sensing meter connected with the electronic control system is arranged in the chromatographic column; (c) the waste liquid tank is sealed and connected with a negative pressure pump, and a negative pressure sensor connected with the electronic control system is arranged in the sealed tank. While the functional effect is maintained, the production cost of the negative-pressure, liquid-phase chromatographic instrument is reduced, and the negative-pressure, liquid-phase chromatographic instrument can be widely used to carry out liquid-phase chromatographic analyses in chemical laboratories, medicine laboratories, etc.

Description

The negative pressure liquid chromatograph
(1) technical field:
The utility model relates to the automated analysis instrument, is a kind of negative pressure liquid chromatograph.
(2) background technology:
Stratographic analysis is called liquid phase chromatography with liquid as the analytical approach of moving phase, high performance liquid chromatograph is high-speed with it in such analytical approach, main usefulness, high selectivity and high sensitivity and become the most representative automated analysis instrument, its structure of existing high performance liquid chromatograph mainly comprises some reagent boxes, adopt fluid flow tube to connect solenoid valve successively thereafter, high pressure pump, mixer, solenoid valve, chromatographic column, the optics colorimetric system, waste liquid tank, and is furnished with electronic control system, whole solvent delivery system adopts high pressure pump to produce normal pressure, promotes solvent and flows along the liquid road.So existing high performance liquid chromatograph, its high pressure transfusion system structure is complicated, and manufacture process requirement is higher; Its high pressure pump cost is higher, and is strict to the withstand voltage degree of whole liquid line seal; And its chromatographic column is the high pressure sealing post, requires to adopt high-intensity material to manufacture.
(3) summary of the invention:
The purpose of this utility model will solve existing high performance liquid chromatograph exactly, and the problem that manufacturing cost is high provides a kind of negative pressure liquid chromatograph.
Concrete scheme of the present utility model is: improve at high performance liquid chromatograph, it includes some reagent boxes equally, adopt fluid flow tube to connect solenoid valve, mixer, solenoid valve, chromatographic column, optics colorimetric system, waste liquid tank successively thereafter, and be furnished with electronic control system, it is characterized in that: a. installs peristaltic pump additional at the chromatographic column leading portion; B. chromatographic column is a unenclosed construction, and is provided with liquid level inductive needle connection electronic control system in chromatographic column; C. waste liquid tank is a sealable tank, connects negative pressure pump is housed, and negative pressure transducer connection electronic control system is set in sealable tank.
The utility model has been cancelled high pressure pump, and solvent adopts peristaltic pump to carry before entering chromatographic column, enters and adopts negative pressure device (pump) drainage downwards after the chromatographic column, and negative pressure makes liquid flow along pipeline; Peristaltic pump and negative pressure device are all simple in structure, and manufacture process requirement is not high, and cost is low; Chromatographic column wherein is an open type, does not have pressure, and cylinder is not had specific (special) requirements, and cost is low, and except that chromatographic column to one section pipeline of waste liquid tank had the better sealing of certain pressure requirement, all the other liquid lines did not have the pressure requirement simultaneously.Therefore the utlity model has similar function of high performance liquid chromatograph and advantage, but manufacturing cost reduces significantly.Negative pressure designed in the utility model can produce negative pressure stably, and for the chromatography process provides the pulse free constant rate, fluid flow can be regulated in a big way continuously, can change moving phase easily under programmed control.Eluent (reagent) adopts flow-type to add, and can realize two kinds of types of elution of normal liquid and gradient, and the combination by solenoid valve can reduce with switching and stepper motor driven peristaltic pump, and dead volume influences in the experiment.Confirm that through a large amount of clinical samples contrast tests this method testing result accuracy is good, precision is high, detection speed is fast.
(4) description of drawings:
Accompanying drawing is a general structure synoptic diagram of the present utility model.
Among the figure: 1-operating platform, 2-single-chip microcomputer, 3-control circuit, 4,5, the 6-reagent box, 7,8, the 9-three-way solenoid valve, 10-reagent peristaltic pump, the 11-mixer, 12-three-way solenoid valve, 13-application of sample peristaltic pump, 14-sample/reagent needles, 15-liquid level inductive needle, 16-chromatographic column, 17-cleans water tank, and 18, the 19-three-way solenoid valve, 20-emptying peristaltic pump, the 21-light source, the mobile printing opacity sample cell of 22-trace, 23-grating, 24-photoelectric detector, the 25-two way solenoid valve, 26-discharges peristaltic pump, 27-waste liquid tank, 28-negative pressure transducer, the 29-three-way solenoid valve, the 30-negative pressure pump.
(5) embodiment:
Referring to accompanying drawing, the utility model comprises three reagent boxes 4,5,6, three kinds of reagent are housed respectively, three reagent boxes 4,5,6 respectively by three arms and three three-way solenoid valves 7,8,9 (each solenoid valve has an end blowing air) connect main fluid flow tube, connect reagent peristaltic pump 10 more successively, mixer 11, three-way solenoid valve 12, the two ends in addition of three-way solenoid valve 12, one termination application of sample peristaltic pump 13 (being used for application of sample), the other end is communicated with chromatographic column 16 by sample/reagent needles 14, this chromatographic column 16 is an open type, need not sealing, be provided with liquid level inductive needle 15 in the chromatographic column 16 and connect electronic control system.Chromatographic column 16 outlets connect respectively by three-way solenoid valve 18 cleans water tank 17 (being used for detergent line) and three-way solenoid valve 19, three-way solenoid valve 19 connects the mobile printing opacity sample cell 22 of trace of emptying peristaltic pump 20 (being used for emptying) and optics colorimetric system more respectively, optics colorimetric system in the utility model also includes light source 21, grating 23, photoelectric detector 24, trace flows printing opacity sample cell 22 by two way solenoid valve 25 connection waste liquid tanks 27, waste liquid tank 27 is a sealable tank, and by three-way solenoid valve connection negative pressure pump 30, and in waste liquid tank 27, be provided with negative pressure transducer 28 connection electronic control systems.Be convenient discharging waste liquid, delivery pipe and discharging peristaltic pump 26 are installed in the bottom of waste liquid tank 27, dispose the electronic control system of forming by operating platform 1, single-chip microcomputer 2, control circuit 3 at last and control each solenoid valve in the utility model, peristaltic pump, negative pressure pump, photoelectric detector etc. respectively and promptly constitute the utility model.
Principle of work of the present utility model: at first carry out parameter setting, comprise the setting of 1. sample sizes: the button input, instrument rotates the number of turns by control application of sample peristaltic pump 13 stepper motors and regulates; 2. reagent type and addition: the button input, instrument rotates the number of turns by control reagent solenoid valve 12 and reagent peristaltic pump 10 stepper motors and regulates; 3. scavenging period is set: the button input, instrument is by regulating cleaning valve opening time control scavenging period.
Workflow of the present utility model comprises:
1. application of sample: sample/reagent needles 14 is inserted in the samples, and button starts application of sample peristaltic pump 13 backward rotation, and sample sucks in the pin, and hummer prompting sample is drawn and finished, and application of sample peristaltic pump 13 forward rotation are injected chromatographic column 16 with sample;
2. add reagent: according to the parameter selectively unlocking reagent solenoid valve 19 that presets, reagent peristaltic pump 10 rotates, and reagent flow to chromatographic column 16;
3. negative pressure drainage chromatography: reagent flows in the chromatographic column 16, level sensing pin 15 is sensed behind the reagent signal feedback to control circuit 3, control circuit 3 is opened negative pressure pump 30 and solenoid valve 29, and the reagent of adding flow to waste liquid tank 27 through the chromatographic column core along pipeline under suction function.Negative pressure transducer 28 is used to monitor the pressure size in the negative waste liquid tank 27, and will sense feed back to control circuit 3 (screen shows in real time), negative pressure pump 30 quits work when pressure exceeds setting value, negative pressure pump 30 restarted when pressure was lower than setting range;
4. spectrophotomelric assay: flow of liquid is when trace flows printing opacity sample cell 22 in the negative pressure chromatography process, liquid carries out real-time colorimetric in 24 pairs of sample cells of photoelectric detector, and will detect data-switching and become electric signal to reach control circuit 3, carry out calculation process by single-chip microcomputer 2, at last with screen display or external workstation on the output function platform 1 as a result;
5. solenoid purge valve 18 was opened after detection was finished, and the clean-out system that cleans in the water tank 17 flow to waste liquid tank 27 at suction function lower edge pipeline, and pipeline is played cleanup action; After cleaning was finished, negative pressure pump 30 quit work, and the three-way solenoid valve 29 of negative pressure line is closed the negative pressure mouth, opens air scoop, and this moment, a jar interior negative pressure released, and started discharging peristaltic pump 26 and emptying peristaltic pump 20 emptying waste liquids then.This cleaning procedure can be provided with instrument when each open and close machine carries out 1-3 time automatically, with the cleaning on maintenance instrument fluid flow tube road.

Claims (1)

1. negative pressure liquid chromatograph, have some reagent boxes, thereafter adopt fluid flow tube to connect solenoid valve, mixer, solenoid valve, chromatographic column, optics colorimetric system, waste liquid tank successively, and be furnished with electronic control system, it is characterized in that: a. installs peristaltic pump additional at the chromatographic column leading portion; B. chromatographic column is a unenclosed construction, and is provided with liquid level induction meter connection electronic control system in chromatographic column; C. waste liquid tank is a sealable tank, connects negative pressure pump is housed, and negative pressure transducer connection electronic control system is set in sealable tank.
CNU2008200652170U 2008-01-01 2008-01-01 Negative-pressure liquid phase chromatograph Expired - Fee Related CN201133904Y (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CNU2008200652170U CN201133904Y (en) 2008-01-01 2008-01-01 Negative-pressure liquid phase chromatograph

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNU2008200652170U CN201133904Y (en) 2008-01-01 2008-01-01 Negative-pressure liquid phase chromatograph

Publications (1)

Publication Number Publication Date
CN201133904Y true CN201133904Y (en) 2008-10-15

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ID=40062173

Family Applications (1)

Application Number Title Priority Date Filing Date
CNU2008200652170U Expired - Fee Related CN201133904Y (en) 2008-01-01 2008-01-01 Negative-pressure liquid phase chromatograph

Country Status (1)

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CN (1) CN201133904Y (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101614711B (en) * 2009-04-03 2012-07-04 天津博纳艾杰尔科技有限公司 Automatic three-stage chromatograph
CN107525708A (en) * 2016-06-21 2017-12-29 上海炫电气有限公司 A kind of sample gas dilution system being operable with subnormal ambient

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101614711B (en) * 2009-04-03 2012-07-04 天津博纳艾杰尔科技有限公司 Automatic three-stage chromatograph
CN107525708A (en) * 2016-06-21 2017-12-29 上海炫电气有限公司 A kind of sample gas dilution system being operable with subnormal ambient
CN107525708B (en) * 2016-06-21 2019-12-24 上海炫一电气有限公司 Sample gas dilution system capable of working in negative pressure environment

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