CN204874521U - Cell collection device - Google Patents

Abstract

The utility model proposes a cell collection device, which device includes a filtrate collection bottle and a filter arranged on the top of the filtrate collection bottle; the filter is composed of a container for holding samples, a filter device and a piston device; the The filtering device is arranged at the lower part of the container; the piston device extends into the container; and the pressure device is arranged at the upper part of the filter. The device of the utility model has simple structure and low cost, and is suitable for popularization in rural areas; as can be seen from the use of the device, the device is easy to operate, fast, improves work efficiency, and reduces labor intensity; and improves the positive rate of inspection, Reduced costs.

Description

cell collection device

technical field

The utility model relates to the field of medical equipment, in particular to a cell collection device.

Background technique

One of the frontiers in the development of early cancer diagnosis and monitoring today is how to use blood, urine or other bodily fluids for cancer diagnosis and screening. The biggest advantage of this technology is: non-invasive detection, which can efficiently diagnose cancer in the early stage of cancer and can be used repeatedly in a short period of time, so as to achieve real-time monitoring. In addition, it prevents patients from receiving unnecessary biopsies, and the detection cost is relatively cheap, which is conducive to wide application in clinical practice. With the development of science and technology, the accuracy and sensitivity of this detection technology have been greatly improved in recent years. However, how to separate extremely small amounts of cancer cells from a large amount of body fluids easily and quickly is a problem that current researchers are trying to solve.

Previously, the traditional centrifugation method was used to collect cells. Put the cells to be collected into the test tube and centrifuge them routinely. However, some deficiencies have been found through long-term clinical practice: after the test tube is centrifuged, when the supernatant is poured out, it is not clean at one time, but it needs to be poured back and forth repeatedly. Such a result makes the cells that have been fully enriched after centrifugation mixed with the remaining liquid (because the supernatant is manually operated, it cannot be poured very clean) in the repeated pouring process, so that the cells that have been centrifuged The centrifuged cells were diluted again. Every time the supernatant is poured out, the cells will be lost, which will greatly reduce the number of cells finally obtained, reduce the validity of the sample and the clinical positive rate, and greatly increase the work intensity of the experimenters. Also, this method cannot separate different types of cells.

In view of this, it is necessary to develop a cell collector that is easy to operate and reduces labor intensity.

Utility model content

The utility model provides a cell collection device, which solves the problem that cells cannot be collected quickly and easily in the prior art.

The technical scheme of the utility model is achieved in that:

A cell collection device, comprising a filtrate collection bottle and a filter arranged on the top of the filtrate collection bottle;

The filter is composed of a container for holding samples, a filter device and a piston device;

The filtering device is arranged at the lower part of the container; the piston device extends into the inside of the container;

A pressure device is provided on the upper part of the filter.

As a preferred technical solution, the filter is a syringe filter.

As a preferred technical solution, the pressure device is an elastic band, the two sides of the elastic band are respectively fixed on both sides of the filtrate collection bottle, and the middle part of the elastic band is placed on the top of the piston device.

As a preferred technical solution, the filtering device is conical or wedge-shaped.

As a preferred technical solution, the filter device includes a support, a filter membrane and a cell collection tray, the filter membrane is attached to the inside of the support, and the cell collection tray is arranged at the bottom of the support.

As a preferred technical solution, the filter membrane is a 5 μm filter membrane or a 2 μm filter membrane.

As a preferred technical solution, an air plug is provided between the piston device and the sample plane. Among them, the capacity of the air plug is about 8-15ml; the air plug can be set to other volumes according to the demand; the size of the device can be changed according to the sample volume, the viscosity of the body fluid and the number of cells contained.

As a preferred technical solution, a sealing device is provided inside the container.

A method of using the cell collection device described above, comprising:

Place the sample into the filter;

The piston device is placed in the filter; an air plug is formed between the piston device and the sample;

Place the filtrate collection bottle on the lower part of the filter;

Press the plunger to move down, so that all the samples and most of the air are filtered through the filter membrane, and the filtrate is collected to obtain the required test product.

The above-mentioned technical scheme described in the utility model has the beneficial effects obtained with respect to the prior art as follows:

(1) The device has simple structure and low cost, and is suitable for popularization in rural areas;

(2) From the use of the device, it can be seen that the device is easy to operate and fast, improves work efficiency and reduces labor intensity; and improves the positive rate of inspection and reduces costs.

Description of drawings

In order to illustrate the utility model embodiment or the technical solution in the prior art more clearly, the accompanying drawings that need to be used in the description of the embodiment or the prior art will be briefly introduced below. Obviously, the accompanying drawings in the following description These are only some embodiments of the present utility model, and those skilled in the art can also obtain other drawings according to these drawings without any creative effort.

Fig. 1 is the structural representation of a kind of cell phone device of the present utility model;

in:

1-cell collection device, 11-filtrate collection bottle, 12-filter, 13-pressure device, 121-container, 122-filter device, 123-piston device, 124-air plug, 1221-stent, 1222-filter membrane, 1223 - Cell collection tray.

Detailed ways

The following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

The principle of the utility model is: adopting the method of physical solution retention design and surface chemical treatment to improve the efficiency of capturing a small amount of cells in the solution.

As shown in FIG. 1 : the cell collection device 1 includes a filtrate collection bottle 11 and a filter 12 . The filter 12 is arranged on the top of the filtrate collection bottle 11 . Its filter 12 is made up of the container 121 that holds sample, filter device 122 and piston device 123, and wherein filter device 122 is arranged on the bottom of container 121; The lower part of 123 is arranged inside the container 121, the upper part of the piston 123 is located outside the container 121, and the piston 123 is outside the container 121 when it is not applicable. There is no connection between the two when it is not applicable, and it is an independent unit. body. The container 121 is mainly used to place solution samples such as urine. The filter 12 in the present embodiment selects the syringe filter, so the filter device 122 is conical or wedge-shaped; Inside, the cell collection plate 1223 is set at the bottom of the bracket 1221, and the filter membrane 1222 is selected according to the requirements of the filtered sample. In this embodiment, the filtered sample is urine, so the selected filter membrane 1222 is a 5 μm filter membrane. Other cells (including red blood cells and white blood cells) pass through the 5 μm filter membrane 1222 along with the filtrate, while prostate epithelial cells (diameter 15-40 μm) are large, so they cannot pass through the filter membrane and are enriched on the collection plate. When the pore size of the filter membrane is 2 μm, all the cells cannot pass through the filter membrane and are enriched on the collection plate. That is, the 5μm filter is used to trap epithelial or cancer cells; and the 2μm filter is used to trap all cells. There is an air plug 124 between the piston device 123 and the sample plane, and the capacity of the air plug is about 10ml; the air plug can be set to other volumes according to the demand; the size of the device can be determined according to the sample volume, the viscosity of the body fluid and the cell content. change in quantity. In order to promote the movement of the piston device 123, a pressure device 13 is provided on the top of the filter 12, wherein the pressure device 13 can also be selected according to the habits of those skilled in the art, and the pressure device 13 selected in this embodiment is an elastic band. The two sides of 13 are respectively fixed on both sides of the filtrate collection bottle 11, and the middle part of the elastic band is placed on the top of the piston device 123. When the pressure device 13 presses the piston to move downward, all the urine samples and most of the air plug 124 are filtered through the filter membrane. , only a small amount of liquid remains in the cell collection tray 1223. For better filtration, a sealing device, namely a sealing ring, is provided inside the container 121 .

The following describes how to use the above cell collection device:

The working process of the device is illustrated by taking the isolation of prostate epithelial cells (including cancer cells) from urine as an example.

Add 50ml of urine sample into the syringe filter, wherein the urine, particles with a diameter less than 10 μm and blood cells will enter the filtrate collection bottle 11 through the filter membrane with the urine. The piston device 123 is inserted into the upper part of the syringe filter, and an air plug of about 10 ml is formed between the urine and the piston device 123 . Place the elastic bands fixed on both sides of the filtrate collection bottle 11 on the top of the piston device 123 . The elasticity of the elastic band will press the piston device 123 to move downward, so that all the urine samples and most of the air plugs filter the filter membrane 1222, leaving only a small amount of liquid in the collection tray 1223. During this process, prostate epithelial cells (15-40 μm in diameter) are larger than other cells in urine (including red blood cells, white blood cells), unable to pass through the 5 μm filter membrane, trapped on the filter membrane, and enriched to the bottom of the filter due to gravity in the collection tray 1223. The collection plate 1223 is hydrophilic and has evenly cross-linked antibodies on its surface. The choice of antibody is determined by the antigen expressed on the surface of the cells to be harvested. For example, to separate prostate epithelial cells from urine, the collection plate 1223 will cross-link the antibody of prostate epithelial cell surface antigen PCMA to affinity-adsorb the prostate epithelial cells expressing PCMA. During the filtering process, the prostate epithelial cells are affinity-adsorbed by the antibody and fixed on the collection plate 1223 . The collection dish 1223 with an area of 10 square millimeters can collect up to 25,000 prostate epithelial cells with a diameter of 20 microns. After the filtration, 20 to 50 microliters of liquid remain on the collection dish 1223 to protect the cells adsorbed on the collection dish 1223 from losing water. After filtering, loosen the elastic band and pull out the piston device 123 . Add 15ml of flushing solution to the syringe filter and repeat the filtration process to elute residual urine. Finally, 1 ml of rinse solution was added to rinse the collection tray 1223 . Using a long-nosed pipette, aspirate the rinsate and any other cells that are not affinity bound to the collection plate. At this time, the cells collected in the collection dish 1223 can be directly lysed to extract RNA.

The above-mentioned filtration and flushing process takes about 20 minutes, of which the operation time is 5 minutes. Using this device to collect cells in solution saves 40 minutes compared with the current commonly used method; and can improve collection efficiency and sample quality.

From the above use process, it can be known that the device is easy to operate and fast, improves work efficiency and reduces labor intensity; moreover, it improves the positive rate of inspection and reduces the cost.

The above descriptions are only preferred embodiments of the present utility model, and are not intended to limit the present utility model. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present utility model shall be included in the Within the protection scope of the present utility model.

Claims (9)

1. A cell collection device, characterized in that it comprises a filtrate collection bottle and a filter arranged on the top of the filtrate collection bottle; The filter is composed of a container for holding samples, a filter device and a piston device; The filtering device is arranged at the lower part of the container; the piston device extends into the inside of the container; A pressure device is provided on the upper part of the filter. 2. A cell collection device according to claim 1, wherein the filter is a syringe filter. 3. A cell collection device according to claim 1, wherein the pressure device is an elastic band, the two sides of the elastic band are respectively fixed on both sides of the filtrate collection bottle, and the middle part of the elastic band is placed on top of the piston assembly. 4. A cell collection device according to claim 1, wherein the filter device is conical or wedge-shaped. 5. A cell collection device according to claim 4, wherein the filter device comprises a support, a filter membrane and a cell collection tray, the filter membrane is attached to the inside of the support, and the cell collection tray is set at the bottom of the stand. 6 . The cell collection device according to claim 5 , wherein the filter membrane is a 5 μm filter membrane or a 2 μm filter membrane. 7. A cell collection device according to claim 1, wherein an air plug is provided between the piston device and the sample plane. 8. A cell collection device according to claim 7, characterized in that the capacity of the air plug is 8-15ml. 9. A cell collection device according to claim 1, wherein a sealing device is provided inside the container.