CN213912452U

CN213912452U - Macroporous resin chromatographic column device

Info

Publication number: CN213912452U
Application number: CN202022331133.0U
Authority: CN
Inventors: 唐少旗; 豆文科; 蔡洋
Original assignee: Baoji Chenguang Bio Tech Co ltd
Current assignee: Baoji Chenguang Bio Tech Co ltd
Priority date: 2020-10-20
Filing date: 2020-10-20
Publication date: 2021-08-10
Anticipated expiration: 2030-10-20

Abstract

The utility model discloses a macroporous resin chromatographic column device, including being equipped with macroporous resin and being transparent structure's cylinder, the cylinder intercommunication has extract valve pipe and mixes eluent valve pipe device to be provided with in the cylinder can dismantle sealed, and be used for the micropore baffle that blocks macroporous resin. The vacuum pump respectively extracts the traditional Chinese medicine extracting solution and a plurality of eluents of different types into the column body and the corresponding liquid storage tank. Different eluents can be mixed in real time through the proportional valve, the elution proportion is not required to be configured in advance, the labor and time are saved, and the working efficiency is improved. The baffle plate is provided with a micropore through hole, so that the extracting solution and the eluent can pass through the baffle plate, the particle substances contained in the extracting solution and the eluent can be filtered, and the service life of the macroporous resin is prolonged. In addition, the baffle can prevent the macroporous resin from floating upwards to reduce the column effect during liquid inlet, the height of the baffle can be randomly lifted, the loading of the macroporous resin is adjusted, and the baffle is convenient and quick to use and is suitable for popularization and use.

Description

Macroporous resin chromatographic column device

Technical Field

The utility model relates to a traditional chinese medicine draws technical field, especially relates to a macroporous resin chromatographic column device.

Background

The separation and purification of effective components in traditional Chinese medicine is an important process link in the modernization process of traditional Chinese medicine, is an important means for improving the effective components of traditional Chinese medicine, and is especially widely applied in the field of natural pharmaceutical chemistry. At present, the means adopted in the extraction, separation and purification of chemical components of traditional Chinese medicines in the market comprise a macroporous resin column, a silica gel chromatographic column, a high performance liquid chromatographic column, water extraction and alcohol precipitation, extraction, membrane separation and the like, wherein the macroporous adsorption resin technology has the advantages of stable physicochemical properties, large specific surface area, large adsorption capacity, high selectivity, high adsorption speed, mild desorption conditions, easy regeneration and the like, and shows great superiority in the separation and purification of the effective components of the traditional Chinese medicines.

The macroporous adsorption resin is a high molecular adsorption resin which does not contain exchange groups and has a macroporous structure, has a good macroporous network structure, and can selectively adsorb different substances. The macroporous adsorption resin is in tiny particle shape, the resin is easy to wash up when the traditional Chinese medicine extracting solution or the eluent is added, so that the effective components of the traditional Chinese medicine are not uniformly and completely adsorbed, and the proportion of the eluent is required to be manually configured at present, and then the eluent is manually added onto a chromatographic column to separate and purify the effective components of the traditional Chinese medicine, so that time and labor are wasted.

Disclosure of Invention

To the problem that exists, the utility model aims at providing a macroporous resin chromatographic column device, the vacuum pump can draw different kinds of eluents respectively automatically and store in the liquid storage pot, and the automatic cylinder that adds of traditional chinese medicine extract to the mixing proportion of accessible proportional valve automatically regulated eluant saves time and energy. And the baffle that sets up in the cylinder can effectively block the come-up of macroporous resin, improves the elution effect of extract.

In order to achieve the above object, the utility model adopts the following technical scheme: the utility model provides a macroporous resin chromatographic column device, is including being equipped with macroporous resin and being the cylinder of transparent structure and cylinder top detachable lid, the liquid valve pipe that the cylinder bottom was seted up, wherein be equipped with vacuum pump, main line and blow vent on the lid, it has extract valve pipe and mixed eluant valve pipe device to connect in parallel between main line and the lid transversely be provided with in the cylinder can dismantle sealed, and be used for the micropore baffle that blocks macroporous resin.

Preferably, the mixed eluent valve tube device comprises a plurality of different types of eluent devices with the same structure and connected in parallel on a main pipeline, and a mixing device connected with the cover body, and one sides of the plurality of eluent devices close to the cover body are all connected into the mixing device in a gathering manner.

Preferably, each eluent device comprises a valve and a single liquid storage tank which are sequentially connected in series, and the mixing device comprises a proportional valve and a mixing liquid storage tank which are used for proportionally mixing the eluents in different single liquid storage tanks.

Preferably, the top surface of the microporous baffle is provided with compression bars which are elastically compressed on the inner wall of the column body and used for keeping the baffle in a positioning state at intervals along the circumferential direction.

The utility model has the advantages that: (1) the utility model can be used for the adsorption, impurity removal and purification of the traditional Chinese medicine extract and the cleaning and regeneration of resin. The vacuum pump respectively extracts the traditional Chinese medicine extracting solution and a plurality of eluents of different types into the column body and the corresponding liquid storage tank. Different eluents can be mixed in real time through the proportional valve, the elution proportion is not required to be configured in advance, the labor and time are saved, and the working efficiency is improved. (2) The baffle plate is provided with a micropore through hole, so that the extracting solution and the eluent can pass through the baffle plate, the particle substances contained in the extracting solution and the eluent can be filtered, and the service life of the macroporous resin is prolonged. In addition, the baffle can prevent the macroporous resin from floating upwards to reduce the column effect when liquid is fed, the height of the baffle can be randomly lifted, the loading of the macroporous resin is adjusted, the use is convenient and quick, the baffle is suitable for the amplification production of laboratory pilot scale test, pilot scale test and factories, the practicability is high, and the baffle is suitable for popularization and use.

Drawings

Fig. 1 is a schematic view of the whole structure of the chromatographic column device of the present invention.

Detailed Description

In order to make those skilled in the art better understand the technical solution of the present invention, the following describes the technical solution of the present invention with reference to the accompanying drawings and embodiments.

Referring to the figure 1 a macroporous resin chromatographic column device that shows, including being equipped with macroporous resin 100 and being cylinder 1 of transparent structure and cylinder 1 top detachable lid 11, the play liquid valve pipe 2 that 1 bottom of cylinder was seted up, wherein be equipped with vacuum pump 3, main line 4 and blow vent 101 on the lid 11, parallelly connected has extract valve pipe 5 and mixed eluant valve pipe device between main line 4 and the lid 1 transversely be provided with in the cylinder 1 can dismantle sealed, and be used for the micropore baffle 6 that blocks macroporous resin 100, it not only can supply liquid to pass through, can also filter the insoluble impurity in the extract, and preferred have acidproof, alkali, high temperature, oxidation-resistant silica gel material at micropore baffle 6 overcoat. The use method of the device comprises the following steps: firstly, the cover body 11 is opened, macroporous resin with certain capacity is filled in the column body 1, then the microporous baffle 6 is assembled in the column body 1 to block the macroporous resin, the resin is prevented from floating upwards to reduce the column effect when liquid is fed, and the column body 1 is made of glass materials, so that the adsorption condition of a sample in the column body can be observed conveniently at any time. And then closing a cover body 1 on the top end of the column body 1, closing the circulation of the mixed eluent valve pipe device, adding the extracting solution into the column body 1 through a vacuum pump 3 and an extracting solution valve pipe 5, filtering impurities through a microporous baffle 6, flowing onto macroporous adsorption resin for adsorption, then closing the extracting solution valve pipe 5, opening the mixed eluent valve pipe device for circulation, introducing a plurality of required eluents into the mixed eluent valve pipe device, mixing the eluents in proportion, and flowing into the column body 1 for eluting and separating the extracting solution. The liquid outlet valve pipe 2 at the bottom of the column body 1 can control the liquid flow rate flowing through the column body 1.

Specifically, the mixed eluent valve tube device comprises a plurality of different types of eluent devices with the same structure and connected in parallel on the main pipeline 4, and a mixing device connected with the cover body 11, wherein one sides of the plurality of eluent devices, which are close to the cover body 11, are all connected into the mixing device in a gathering manner. And respectively filling a plurality of required eluents into the corresponding eluent devices, mixing different eluents in proportion by a mixing device according to the elution requirements of the extracting solution, and introducing the mixed eluents into the column body for accurate elution so as to achieve the optimal elution effect.

Specifically, each eluent device comprises a valve 7 and a single liquid storage tank 8 which are sequentially connected in series, the mixing device comprises a proportional valve 9 and a mixed liquid storage tank 10 which are used for mixing the eluents in the different single liquid storage tanks 8 in proportion, and the proportional valve 9 can mix the eluents in the different single liquid storage tanks 8 in any proportion. Different eluents are sequentially introduced into the corresponding single liquid storage tanks 8 through the vacuum pump 3, are mixed in the mixed liquid storage tank 10 through the proportional valve 9 and then enter the column body 1 through the valve 7 at the bottom of the mixed liquid storage tank, and the extracting solution is eluted and separated.

In order to prevent the microporous baffle 6 from deflecting to cause the macroporous resin 100 to float and the mixed eluent to contact with the macroporous resin 100 and the extracting solution without filtering through the baffle 6, the top surface of the microporous baffle 6 is provided with compression bars 61 which are elastically compressed on the inner wall of the column body 1 along the circumferential direction of the microporous baffle 6 and are used for keeping the baffle 6 in a positioning state, and the structure for realizing the elastic compression action of the compression bars 61 is as follows: each compression bar 61 is hinged on the top surface of the baffle 6, and a torsion spring (not shown in the figure) is arranged at the hinged point to provide elastic compression action, so that each compression bar 61 is compressed on the side wall of the column body 1 with equal force, the positioning and fastening action of the baffle 6 is improved, and the problem that the baffle 6 deflects is avoided. When the dismounting baffle 6 needs to be installed, the pressure lever 61 is enabled to compress the torsion spring through external force so as to be separated from the inner wall of the column body.

The principle of the utility model is that: a macroporous resin column device is characterized in that treated macroporous adsorption resin is filled into a transparent glass column body 1, a baffle 6 is placed from the top end, a vacuum pump 3 is opened to suck extracting solutions into the column body 1 in sequence through switches of valves 7 on different pipelines, different eluents are sucked into corresponding liquid storage tanks respectively, the proportion of the eluents is adjusted to a required proportion by adjusting a proportional valve, the mixed eluents are placed into a mixed liquid storage tank 10, the valve 7 at the bottom of the mixed liquid storage tank 10 is opened during elution, and proper flow rate is adjusted to carry out elution and separation. The elution ratio is adjusted at any time by controlling the proportional valve 9 until the sample is completely eluted from the resin column. The elution ratio can be adjusted through the proportional valve at any time in the elution process, the liquid outlet valve is controlled, and the flow rate of liquid flowing through the resin column is controlled, so that the optimal separation effect is achieved.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a macroporous resin chromatographic column device, is including being equipped with macroporous resin (100) and cylinder (1) top detachable lid (11) for transparent structure, liquid outlet valve pipe (2) that cylinder (1) bottom was seted up, wherein be equipped with vacuum pump (3), main line (4) and blow vent (101) on lid (11), parallelly connected has extract valve pipe (5) and mixed eluant valve pipe device between main line (4) and lid (11) transversely be provided with in cylinder (1) can dismantle sealed, and be used for micropore baffle (6) that block macroporous resin (100).

2. The macroporous resin chromatographic column device according to claim 1, wherein: the mixed eluent valve pipe device comprises a plurality of different types of eluent devices with the same structure and connected in parallel on a main pipeline (4) and a mixing device connected with a cover body (11), and one sides of the plurality of eluent devices close to the cover body (11) are all connected into the mixing device in a gathering way.

3. The macroporous resin chromatographic column device according to claim 2, wherein: each eluent device comprises a valve (7) and a single liquid storage tank (8) which are sequentially connected in series, and the mixing device comprises a proportional valve (9) and a mixed liquid storage tank (10) which are used for proportionally mixing the eluents in different single liquid storage tanks (8).

4. The macroporous resin chromatographic column device according to claim 3, wherein: and the top surface of the micropore baffle (6) is provided with compression bars (61) which are elastically compressed on the inner wall of the column body (1) and used for keeping the baffle (6) in a positioning state at intervals along the circumferential direction.