CN211896952U - A adsorption equipment for plasmid draws suction filtration - Google Patents
A adsorption equipment for plasmid draws suction filtration Download PDFInfo
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- CN211896952U CN211896952U CN202020290401.6U CN202020290401U CN211896952U CN 211896952 U CN211896952 U CN 211896952U CN 202020290401 U CN202020290401 U CN 202020290401U CN 211896952 U CN211896952 U CN 211896952U
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Abstract
The utility model discloses an adsorption equipment for plasmid draws suction filtration belongs to molecular biology experiment technical field. The adsorption column comprises an adsorption column, wherein a discharge port is formed in the bottom of the adsorption column, a first flow control valve is installed on the discharge port, absorbent cotton is laid on the inner bottom of the adsorption column, quartz sand is laid above the absorbent cotton, and a circular filter membrane is placed above the quartz sand. When the adsorption device is used for extracting plasmids, the elution flow rate of the solution is not too high, the flow rate when the eluent is added is controlled by using the dropping funnel and the second flow control valve, so that the solution layer can be prevented from being compressed, the plasmids can not be separated, and the extraction efficiency is improved to a certain extent; the circular filter paper is placed above the adsorbent layer, so that the adsorbent layer is prevented from being washed up when a solution is added, and the circular filter membrane, the quartz sand and the absorbent cotton are sequentially placed below the adsorbent layer, so that fine particles of the adsorbent layer can be prevented from flowing out, and the impurity content can be effectively reduced.
Description
Technical Field
The utility model belongs to the technical field of the molecular biology experiment, particularly, relate to an adsorption equipment that is used for plasmid nucleic acid to draw suction filtration.
Background
The plasmid is a genetic unit which can be autonomously replicated outside the nucleus of a eukaryotic organism cell or outside a prokaryotic pseudonuclear region, and comprises a circular deoxyribonucleic acid molecule outside a eukaryotic organism cellular organelle and a bacterial cell pseudonuclear region, the artificially constructed plasmid can integrate various useful characteristics, such as containing various single enzyme cutting sites, antibiotic resistance and the like, in widely applied genetic engineering, the artificially constructed plasmid is usually used as a carrier to construct hybrid DNA molecules so as to change the original genetic characteristics of organisms, obtain new varieties and produce new products, and therefore, the quality and the speed of plasmid extraction become the basis and the key of various related experiments.
At present, a white adsorption film is paved at the bottom of an adsorption column in a plasmid extraction kit commonly used in a laboratory, and a plastic pressure pad is arranged on the adsorption film, so that the defects of low extraction efficiency, poor purity and high impurity content exist.
SUMMERY OF THE UTILITY MODEL
In view of the not enough of prior art, the utility model provides an adsorption equipment for plasmid draws suction filtration possesses plasmid extraction efficiency height, purification effectual advantage to solve prior art extraction efficiency low, the relatively poor, the higher problem of impurity content of purity.
For the purpose of realizing improving plasmid extraction efficiency and reducing impurity content, the utility model provides a following technical scheme: the utility model provides an adsorption equipment for plasmid extraction suction filtration, includes the adsorption column, the bottom of adsorption column is equipped with the discharge port, and installs first flow control valve on the discharge port, absorbent cotton has been laid to the interior bottom of adsorption column, and the top of absorbent cotton is spread and is had quartz sand, circular filter membrane has been put to the top of quartz sand, be equipped with the adsorbent layer in the adsorption column, circular filter paper has been laid to the liquid level of adsorbent layer, the closing cap is installed on the top of adsorption column, install second flow control valve on the closing cap, and through second flow control valve fixedly connected with dropping funnel.
As an optimized technical scheme of the utility model, the upper and lower both ends of adsorption column all are equipped with the rubber sleeve, the adsorption column is the transparent jar body.
As an optimal technical scheme of the utility model, the bottom of adsorption column is the bottleneck form, the filter membrane clamping ring has been placed on the circular filter membrane, circular filter membrane adopts the silicon substrate membrane.
As an optimized technical scheme of the utility model, the top of closing cap is equipped with first connector, the closing cap is connected with second flow control valve through first connector.
As a preferred technical scheme of the utility model, dropping funnel's bottom is equipped with the second connector, dropping funnel is connected with second flow control valve through the second connector.
As an optimized technical scheme of the utility model, the top of dropping funnel is equipped with irritates the mouth, and splendid attire eluant in the dropping funnel.
Compared with the prior art, the utility model provides an adsorption equipment for plasmid draws suction filtration possesses following beneficial effect:
(1) when extracting the plasmid, the elution velocity of flow that does not make the solution should not be too fast, uses dropping funnel and second flow control valve to control the velocity of flow when the eluant adds, can avoid compressing tightly the solution stratum, and the plasmid can not be separated, plays certain promotion effect to extraction efficiency.
(2) The circular filter paper is placed above the adsorbent layer, so that the adsorbent layer is prevented from being washed up when a solution is added, and the circular filter membrane, the quartz sand and the absorbent cotton are sequentially placed below the adsorbent layer, so that fine particles of the adsorbent layer can be prevented from flowing out, and the impurity content can be effectively reduced.
Drawings
Fig. 1 is a schematic structural view of the present invention;
fig. 2 is a cross-sectional view of the present invention.
In the figure: 1. an adsorption column; 2. an outlet port; 3. a first flow control valve; 4. absorbent cotton; 5. quartz sand; 6. a circular filter membrane; 7. an adsorbent layer; 8. circular filter paper; 9. sealing the cover; 10. a second flow control valve; 11. a dropping funnel; 12. a rubber sleeve; 13. pressing a membrane; 14. a first connection port; 15. a second connection port; 16. and (4) filling the opening.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-2, the utility model discloses an adsorption device for plasmid extraction and filtration, comprising an adsorption column 1, wherein the bottom of the adsorption column 1 is provided with a discharge port 2, a first flow control valve 3 is arranged on the discharge port 2, absorbent cotton 4 is laid on the inner bottom of the adsorption column 1, quartz sand 5 is laid above the absorbent cotton 4, a round filter membrane 6 is laid above the quartz sand 5, an adsorbent layer 7 is arranged in the adsorption column 1, round filter paper 8 is laid on the liquid surface of the adsorbent layer 7, round filter paper 8 is laid above the adsorbent layer 7, so that the adsorbent layer 7 is not flushed up when adding solution, and the round filter membrane 6, the quartz sand 5 and the absorbent cotton 4 are sequentially arranged below the adsorbent layer 7 to prevent fine particles of the adsorbent layer 7 from flowing out, thereby effectively reducing impurity content, a sealing cover 9 is arranged at the top end of the adsorption column 1, install second flow control valve 10 on the closing cap 9, and through second flow control valve 10 fixedly connected with dropping funnel 11, when extracting the plasmid, the elution velocity of flow that does not make the solution should not be too fast, and the velocity of flow when using dropping funnel 11 and second flow control valve 10 to control the eluant and adding can avoid compressing tightly the solution stratum reticulare, and the plasmid separation can not be opened, plays certain promotion effect to extraction efficiency.
Specifically, the upper and lower both ends of adsorption column 1 are all overlapped and are equipped with rubber sleeve 12, adsorption column 1 is the transparent jar body.
In this embodiment, when the operation, with adsorption column 1 vertical fixation on the support, the support passes through clamping structure and grasps adsorption column 1, can grasp in the position department of rubber sleeve 12, is favorable to playing certain cushioning nature to adsorption column 1.
Specifically, the bottom of adsorption column 1 is the bottleneck form, filter membrane clamping ring 13 has been placed on circular filter membrane 6, circular filter membrane 6 adopts silicon substrate membrane.
In the embodiment, the circular filter membrane 6, the quartz sand 5 and the absorbent cotton 4 are sequentially arranged below the adsorbent layer 7 to prevent fine particles of the adsorbent layer 7 from flowing out, and the filter membrane pressing ring 13 mainly plays a role in increasing the gravity effect of the circular filter membrane 6 and avoids the phenomenon of warping caused by the influence of the flow velocity.
Specifically, a first connection port 14 is formed at the top of the sealing cover 9, and the sealing cover 9 is connected with the second flow control valve 10 through the first connection port 14.
In this embodiment, the sealing cap 9 is connected to the second flow control valve 10 through the first connection port 14, which facilitates later disassembly, cleaning and maintenance.
Specifically, the bottom of dropping funnel 11 is equipped with second connector 15, dropping funnel 11 is connected with second flow control valve 10 through second connector 15.
In this embodiment, the dropping funnel 11 is connected to the second flow control valve 10 through the second connection port 15, which facilitates the later-stage disassembly, cleaning and maintenance.
Specifically, the top of the dropping funnel 11 is provided with a filling opening 16, and the dropping funnel 11 is filled with eluent.
In this embodiment, the dropping funnel 11 is fed with an eluent through the inlet 16, which is advantageous for improving the separation capacity.
The utility model discloses a theory of operation and use flow: when the device is used, eluent is added into the dropping funnel 11 through the filling port 16, the solution is filled into the adsorption column 1, when plasmids are extracted, the elution flow rate of the solution is not too high, the dropping funnel 11 and the second flow control valve 10 are used for controlling the flow rate when the eluent is added, the flow rate is controlled by opening the second flow control valve 10, the solution layer can be prevented from being compressed, the plasmids cannot be separated, a certain improving effect is achieved on the extraction efficiency, the circular filter paper 8 is placed above the adsorbent layer 7, the adsorbent layer 7 can not be flushed when the solution is added, the circular filter membrane 6, the quartz sand 5 and the absorbent cotton 4 are sequentially placed below the adsorbent layer 7, the fine particles of the adsorbent layer 7 can be prevented from flowing out, the impurity content can be effectively reduced, and after separation, the first flow control valve 3 is opened and the particles are discharged through the discharge port 2.
In summary, the adsorption device for plasmid extraction and suction filtration does not make the elution flow rate of the solution not too fast when extracting the plasmids, and uses the dropping funnel 11 and the second flow control valve 10 to control the flow rate when the eluent is added, so that the solution layer can be prevented from being compressed, the plasmids cannot be separated, and the extraction efficiency is improved to a certain extent; the circular filter paper 8 is arranged above the adsorbent layer 7, so that the adsorbent layer 7 is prevented from being washed up when a solution is added, and the circular filter membrane 6, the quartz sand 5 and the absorbent cotton 4 are sequentially arranged below the adsorbent layer 7, so that fine particles of the adsorbent layer 7 can be prevented from flowing out, and the impurity content can be effectively reduced.
Claims (6)
1. The utility model provides an adsorption equipment for plasmid extraction suction filtration, includes adsorption column (1), its characterized in that: the bottom of adsorption column (1) is equipped with discharge port (2), and installs first flow control valve (3) on discharge port (2), absorbent cotton (4) have been laid to the interior bottom of adsorption column (1), and quartz sand (5) have been laid to the top of absorbent cotton (4), circular filter membrane (6) have been laid to the top of quartz sand (5), be equipped with adsorbent layer (7) in adsorption column (1), circular filter paper (8) have been laid to the liquid level of adsorbent layer (7), closing cap (9) are installed on the top of adsorption column (1), install second flow control valve (10) on closing cap (9), and through second flow control valve (10) fixedly connected with dropping funnel (11).
2. The adsorption device for plasmid extraction suction filtration according to claim 1, wherein: the upper end and the lower end of the adsorption column (1) are respectively sleeved with a rubber sleeve (12), and the adsorption column (1) is a transparent tank body.
3. The adsorption device for plasmid extraction suction filtration according to claim 1, wherein: the bottom of adsorption column (1) is the bottleneck form, filter membrane clamping ring (13) have been placed on circular filter membrane (6), silicon substrate membrane is adopted in circular filter membrane (6).
4. The adsorption device for plasmid extraction suction filtration according to claim 1, wherein: the top of the sealing cover (9) is provided with a first connecting port (14), and the sealing cover (9) is connected with the second flow control valve (10) through the first connecting port (14).
5. The adsorption device for plasmid extraction suction filtration according to claim 1, wherein: the bottom of dropping funnel (11) is equipped with second connector (15), dropping funnel (11) are connected with second flow control valve (10) through second connector (15).
6. The adsorption device for plasmid extraction suction filtration according to claim 1, wherein: the top of dropping funnel (11) is equipped with into mouth (16), and splendid attire eluant in dropping funnel (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020290401.6U CN211896952U (en) | 2020-03-11 | 2020-03-11 | A adsorption equipment for plasmid draws suction filtration |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020290401.6U CN211896952U (en) | 2020-03-11 | 2020-03-11 | A adsorption equipment for plasmid draws suction filtration |
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| CN211896952U true CN211896952U (en) | 2020-11-10 |
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| CN202020290401.6U Active CN211896952U (en) | 2020-03-11 | 2020-03-11 | A adsorption equipment for plasmid draws suction filtration |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116478799A (en) * | 2023-05-15 | 2023-07-25 | 上海威高医疗技术发展有限公司 | Adipose tissue purifying device and purifying method |
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2020
- 2020-03-11 CN CN202020290401.6U patent/CN211896952U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116478799A (en) * | 2023-05-15 | 2023-07-25 | 上海威高医疗技术发展有限公司 | Adipose tissue purifying device and purifying method |
| CN116478799B (en) * | 2023-05-15 | 2024-05-24 | 上海威高医疗技术发展有限公司 | Adipose tissue purifying device and purifying method |
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