CN202223934U - Detachable multi-channel column chromatography device - Google Patents
Detachable multi-channel column chromatography device Download PDFInfo
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- CN202223934U CN202223934U CN2011203440293U CN201120344029U CN202223934U CN 202223934 U CN202223934 U CN 202223934U CN 2011203440293 U CN2011203440293 U CN 2011203440293U CN 201120344029 U CN201120344029 U CN 201120344029U CN 202223934 U CN202223934 U CN 202223934U
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Abstract
The utility model discloses a detachable multi-channel column chromatography device, which comprises a support base and glass columns. Two perpendicular supporting columns are perpendicularly arranged on the support base, a transverse fixing column is perpendicularly disposed in the middles of the two perpendicular supporting columns, second iron clamps capable of moving left and right are arranged on the transverse fixing column, the glass columns can be clamped in the second iron clamps, upper ends and lower ends of the glass columns are respectively connected with glass liquid storage devices and flow speed control switches via glass dull polishing connectors, a cylindrical connecting base is disposed below the flow speed control switches and fixed on the two perpendicular supporting columns via two first iron clamps, a slender glass extending tube extending from each flow speed control switch penetrates through the connecting base to be inserted in a sample liquid collecting bottle, and a glass cylindrical and tubular exhausting nozzle which is connected with a vacuumizing rubber tube is further disposed in the middle of the connecting base. The detachable multi-channel column chromatography device resolves problems that an existing column chromatography device is easy to make mistakes and large in occupied space, and needs to be replaced integrally.
Description
Technical field
The utility model belongs to food, medicine and environmental sample pretreatment technology field, is specifically related to a kind of detachable multi-channel column chromatographic apparatus.
Background technology
Column chromatography (Column Chromatography) is a kind of sample separation technology of classics, through the adsorption and desorption additive process between sample component and the column filling matrix, realizes the separation between the sample different component, reaches clean-up effect.Column chromatography is mainly used in separation, the purifying of sample and concentrates; Compare with the solid phase extraction techniques that developed recently gets up; Its isolation of purified principle is consistent, and solid phase extraction techniques is widely used owing to easy and simple to handle; But the conventional post chromatography since have filler select free, the volume containing the sample range of choice is wide, to characteristics such as complex component good separating effect, use cost are lower, still have irreplaceable effect in fields such as medicine, food, environmental protection.
Column chromatography can be divided into normal-phase chromatography, reversed phase chromatography, ion-exchange chromatography, gel chromatography etc. according to the difference of column filling matrix, can be divided into decompression chromatography, normal pressure chromatography, pressurization chromatography according to the method for operating difference.
The column chromatography technology operating procedure generally was divided into for five steps:
The first step: dress post
Be divided into wet method dress post and dry column-packing, wet method dress post is easier, and dry column-packing is had relatively high expectations to column packing technique but post is imitated better.But no matter which kind of dress post method all requires to adorn pillar in fact, adorn evenly, and big bubble can not be arranged, and can not ftracture.Dress post effect can directly influence the isolation of purified effect.
Second step: the preliminary treatment of chromatographic column (claiming balance columns again)
For the chromatographic column filler that makes filling reaches an equilibrium, uniform state, make the sufficient solvation of filler, to improve the separating effect and the reappearance of chromatographic column, need carry out balance with suitable solvent coupled columns.
The 3rd step: the interpolation of sample (deserving to be called appearance again)
Pillar is after overbalance, and sample (solution or the solid that mixes with suitable filler) is added in the chromatographic column.
The 3rd step: the washing of post (claiming the drip washing of post again)
Select suitable solvent, interfering component in the sample is eluted, analyte still is retained on the post simultaneously.
The 4th step: the wash-out of analyte
Eluant, eluent with suitable elutes analyte, and for improving separating effect, the solvent of selection is answered the least possible interfering component that washes.
Existing column chromatography device is generally the single chromatographic column of buying on the market, and is with low cost, has certain advantage when doing single sample, but not enough below existing:
When (1) making a plurality of sample simultaneously, need operate a plurality of chromatographic columns respectively, make mistakes in chaos easily;
(2) when the needs decompression operation, each chromatographic column all need dispose corollary equipments such as corresponding surge flask, vavuum pump, and cost is higher, and pressure is wayward, and can take bigger lab space;
(3) existing chromatographic column is integral post, and it is convenient inadequately to clean, and place breakage just needs integral replacing, causes waste.
Summary of the invention
The purpose of the utility model provides a kind of detachable multi-channel column chromatographic apparatus, solves the problem that there is easy error in existing column chromatography device, takes up room greatly, needs integral replacing.
The technical scheme that the utility model adopted is; Detachable multi-channel column chromatographic apparatus; Comprise bracket base, be vertically installed with two vertical support column on the bracket base, be provided with the crosswise fixed post perpendicular to two vertical support column middle parts; But the crosswise fixed post is provided with second iron clamp of move left and right; Also comprise the glass cylinder that may be stuck in second iron clamp, the top and bottom of glass cylinder are connected with glass reservoir, flow velocity gauge tap through the first glass frosted interface, the second glass frosted interface respectively, and the below of flow velocity gauge tap is provided with columnar connection pedestal; Connecting pedestal is fixed on two vertical support column through two first iron clamps; The flow velocity gauge tap extends an elongated glass extension, and the glass extension passes and connects pedestal and insert and connect in the appearance liquid receiving flask that the pedestal below is provided with, the middle part that connects pedestal also be provided with one with vacuumize glass cylindrical tubulose that rubber tube the is connected mouth of bleeding.
The characteristics of the utility model also are,
Second iron clamp wherein is provided with two or more.
Glass cylinder wherein is provided with two or more.
The beneficial effect of the utility model is:
(1) simple in structure, easy operating, neat appearance is saved the space.The column chromatography of 1-6 sample of single covering device operation can carrying out is simultaneously handled, and increases work efficiency greatly.
(2) with low cost, each assembly is a glassware, and adopts the segmentation assembling design, and each several part is detachable, is easy to preparation and replacing, and each interface is the design of universal standard frosted mouth, and good airproof performance, highly versatile can freely be assembled easy-maintaining.
(3) appearance on the big volume, drip washing and wash-out operation are convenient in the reservoir design of post upper end, and receiving bottle adopts standard frosted mouth, is convenient to follow-up concentrating.
(4) chromatographic column, connection pedestal, receiving bottle etc. are the manufacturing of overall height boron glass, and the flow velocity gauge tap is selected polytetrafluoroethylmaterial material for use, and whole device light transmission is strong, visual good, neat and artistic, and analyte is not had absorption, does not receive the influence of solvent species.Autonomous channel chromatography, no cross pollution.
(5) select decompression chromatography mode,, can effectively control chromatography speed through regulating system vacuum.Through control column flow rate switch, effectively control the flow velocity of each chromatographic column respectively.
(6) columnar connection foundation design when connecting a plurality of chromatographic columns and receiving bottle, has the effect of surge flask when decompression operation, realized that a plurality of chromatographic columns can a shared pressure regulating equipment.
Description of drawings
Fig. 1 is the structural representation of the detachable multi-channel column chromatographic apparatus of the utility model.
Among the figure, 1. glass reservoir, the 2. first glass frosted interface, 3. glass cylinder, the 4. second glass frosted interface; 5. flow velocity gauge tap, 6. the 3rd glass frosted interface, 7. the 4th glass frosted interface, 8. kind liquid receiving flask 9. extends glass tube; 10. connection pedestal, the 11. tubuloses mouth of bleeding, 12. vacuumize rubber tube, 13. bracket bases; 14. vertical support column, 15. crosswise fixed posts, 16. first iron clamps, 17. second iron clamps.
The specific embodiment
Below in conjunction with the accompanying drawing and the specific embodiment the utility model is elaborated.
The structure of the detachable multi-channel column chromatographic apparatus of the utility model; As shown in Figure 1; Comprise bracket base 13, be vertically installed with two vertical support column 14 on the bracket base 13, be provided with crosswise fixed post 15 perpendicular to two vertical support column 14 middle parts; But crosswise fixed post 15 is provided with second iron clamp 17 of a plurality of move left and right; Also comprise a plurality of glass cylinders 3, utilize second iron clamp, 17 fixing glass cylinders 3 after regulating the position, the top and bottom of each glass cylinder 3 are connected with glass reservoir 1, flow velocity gauge tap 5 through the first glass frosted interface 2, the second glass frosted interface 4 respectively; The below of flow velocity gauge tap 5 is provided with columnar connection pedestal 10; Connect pedestal 10 and be fixed on two vertical support column 14 through two first iron clamps 16, flow velocity gauge tap 5 extends an elongated glass extension 9, and glass extension 9 passes and connects pedestal 10 and insert and connect 15～20mm in the appearance liquid receiving flask 8 that pedestals 10 belows are provided with.The middle part that glass connects pedestal 10 also be provided with one with vacuumize glass cylindrical tubulose that rubber tube 12 the is connected mouth 11 of bleeding.
The technical basic principle requirement that meets chromatography of the utility model, clastotype comprises anti-phase, positive, ion-exchange, gel infiltration etc., can select different fillers to carry out the chromatography experiment as required.
The operation principle of the detachable multi-channel column chromatographic apparatus of the utility model is normal pressure or decompression chromatography, is applicable to that normal pressure or decompression column chromatography for separation purify.Column chromatography is a kind of chromatographic separation technology of classics, and it comprises the physical chemistry extraction process of liquid phase and solid phase, through the adsorption and desorption additive process between sample component and the column filling matrix, realizes the separation between the sample different component, reaches clean-up effect.When using chromatographic column; Object at first is attracted to the solid column filling surface; The partial impurities component flows out pillar with sample mother liquor and eluent solution, selects appropriate solvent that object is eluted then, thereby reaches the enrichment of the isolation of purified and the object of sample.Column chromatography and traditional ones method compare, and clean-up effect is better, selectivity is stronger, and the fields such as separation, Pharmaceutical Analysis, food analysis, environmental analysis of extracting at natural drug have more widely uses.
The detail operations step of the utility model:
1. device is installed:
Choosing the position puts fixed support well; Utilize first iron clamp 16 to fix cylindrical shape and connect pedestal 10; Through the 3rd glass frosted interface 6 flow velocity gauge tap 5 is fixed on the connection pedestal 10; Through the second glass frosted interface 4 glass cylinder 3 is connected on the flow velocity gauge tap 5 then, and, through the first glass frosted interface 2 glass reservoir 1 is connected glass cylinder 3 tops again with iron clamp 17 fixing glass cylinders 3; Through the 4th glass frosted interface 7 appearance liquid receiving flask 8 is connected connection pedestal 10 belows; To vacuumize rubber tube 12 at last and be connected and connect on the pedestal 10, will guarantee each glass component ground contact during installation closely, non-loosening through the glass cylindrical tubulose mouth 11 of bleeding.
2. dress post:
Can select wet method dress post or dry column-packing.At first reach during selection wet method dress post and go up appearance and the definite dress of drip washing condition post solvent according to filling property, with column packing and the abundant stirring and evenly mixing of solvent, the eliminating bubble; In chromatographic column, add the dress post solvent of about 1/3rd column volumes, open the column flow rate gauge tap, at the uniform velocity pour in the chromatographic column with the mixture of filler and solvent is disposable; Let filler free settling; Guarantee that solvent can not drain off, after sedimentation was accomplished, it was closely knit to guarantee cylinder suitably to reduce pressure.Do not need solvent during dry column-packing, with evengranular filler at the uniform velocity, balanced being packed in the chromatographic column, the limit rim knocks post jamb, guarantees that cylinder is even, closely knit.
3. balance columns:
Select suitable solvent balance chromatographic column, open the column flow rate gauge tap, earlier with the solvent of about 2～3 times of column volumes at the uniform velocity pass through chromatographic column; Guarantee that solvent floods cylinder; Close the column flow rate gauge tap, leave standstill more than 1 hour, to improve the separating effect and the reappearance of chromatographic column.
4. go up appearance:
Pillar is after overbalance, and sample (solution or the solid that mixes with suitable filler) is added in the chromatographic column.For improving clean-up effect, the volume of sample solution or solid should be as much as possible little, but can reservoir be connected the post upper end in case of necessity, carries out appearance on the big volume of liquid.The amount of sample can not surpass the payload of chromatographic column.
When utilizing this device to carry out the solid phase dispersion extraction, need not operate for the 2nd～4 step, directly the mixture of sample and filler packed in the post, carry out follow-up drip washing and wash-out and operate.
5. the drip washing of post
Open the column flow rate gauge tap, select suitable solvent to carry out the drip washing of post,, in case of necessity reservoir is connected the post upper end, carry out big volume drip washing through column flow rate gauge tap and decompressor control flow velocity.This step elutes part interfering component in the sample, and analyte still is retained on the post simultaneously.
The post in the 2nd～5 step flows out liquid and all inserts in the waste collection bottle.
6. the wash-out of analyte
Select suitable eluant, eluent, open the column flow rate gauge tap,, in case of necessity reservoir is connected the post upper end, carry out big volume wash-out through column flow rate gauge tap and decompressor control flow velocity.This step elutes analyte, and for improving separating effect, the solvent of selection is answered the least possible interfering component that washes.
To the deficiency that existing column chromatography device exists, the utility model has following technical characterstic:
The first, on same connection pedestal, can install, use a plurality of chromatographic columns simultaneously, a plurality of chromatographic columns can a shared pressure regulating equipment, and whole device is neat, attractive in appearance, can simplify experimental implementation, saves lab space.
Second; The flow velocity gauge tap of the cylinder of each chromatographic column and the reservoir of upper end, lower end, be connected pedestal and receiving bottle separates, the frosted interface through standard during use links to each other, and is convenient to installation, dismounting, the cleaning of chromatographic column; Easy to use; And can change respectively after cylinder, reservoir, switch, pedestal or the receiving bottle breakage, need not change whole device, can practice thrift use cost.
The 3rd, each interface of device links to each other through the frosted interface of standard, and good airproof performance, compatible good is convenient to decompression operation, and can take combination as required apart and use.
The 4th; Each chromatographic column all has independent gauge tap, can select to use a chromatographic column or use many chromatographic columns simultaneously separately, and different chromatographic columns can carry out different operation; And any one chromatographic column can not influence the use of other chromatographic columns when fault such as obstruction takes place.
The 5th, the flow velocity gauge tap is selected polytetrafluoroethylmaterial material for use, has that inertia is strong, the characteristics of good airproof performance.
The 6th, the specific requirement that can handle per sample, regulating system vacuum, control chromatography speed.
The 7th, each chromatographic column is the autonomous channel, is independent of each other, no cross pollution.
Claims (3)
1. detachable multi-channel column chromatographic apparatus; It is characterized in that; Comprise bracket base (13); Be vertically installed with two vertical support column (14) on the bracket base (13); Be provided with crosswise fixed post (15) perpendicular to two vertical support column (14) middle parts, crosswise fixed post (15) but be provided with second iron clamp (17) of move left and right, also comprise the glass cylinder (3) that may be stuck in second iron clamp (17); The top and bottom of glass cylinder (3) are connected with glass reservoir (1), flow velocity gauge tap (5) through the first glass frosted interface (2), the second glass frosted interface (4) respectively; The below of flow velocity gauge tap (5) is provided with columnar connection pedestal (10), connects pedestal (10) and is fixed on two vertical support column (14) through two first iron clamps (16), and flow velocity gauge tap (5) extends an elongated glass extension (9); Glass extension (9) passes and connects pedestal (10) and insert and connect in the appearance liquid receiving flask (8) that pedestal (10) below is provided with, the middle part that connects pedestal (10) also be provided with one with vacuumize glass cylindrical tubulose that rubber tube (12) the is connected mouth (11) of bleeding.
2. detachable multi-channel column chromatographic apparatus according to claim 1 is characterized in that described second iron clamp (17) is provided with two or more.
3. detachable multi-channel column chromatographic apparatus according to claim 1 is characterized in that described glass cylinder (3) is provided with two or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203440293U CN202223934U (en) | 2011-09-14 | 2011-09-14 | Detachable multi-channel column chromatography device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203440293U CN202223934U (en) | 2011-09-14 | 2011-09-14 | Detachable multi-channel column chromatography device |
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| Publication Number | Publication Date |
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| CN202223934U true CN202223934U (en) | 2012-05-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011203440293U Expired - Fee Related CN202223934U (en) | 2011-09-14 | 2011-09-14 | Detachable multi-channel column chromatography device |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103446778A (en) * | 2013-09-02 | 2013-12-18 | 浙江工业大学 | Ion exchange resin chromatography method and device without being promoted by external power |
| CN104330492A (en) * | 2014-10-31 | 2015-02-04 | 华南理工大学 | Intelligent automatic switching multi-channel ion chromatographic column connecting device |
| CN106730999A (en) * | 2017-03-21 | 2017-05-31 | 天津科技大学 | One kind plus-minus pressure double-purpose operates desk-top chromatographic column |
| CN109669036A (en) * | 2019-02-28 | 2019-04-23 | 深圳市易瑞生物技术股份有限公司 | A kind of push type multichannel chromatographic apparatus |
| CN113713428A (en) * | 2021-06-30 | 2021-11-30 | 上海海路生物技术有限公司 | Chromatographic column extension device, system and method for packing column by using same |
| CN115738381A (en) * | 2022-11-08 | 2023-03-07 | 中国科学院过程工程研究所 | Multi-channel column chromatography device capable of automatically loading and eluting |
-
2011
- 2011-09-14 CN CN2011203440293U patent/CN202223934U/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103446778A (en) * | 2013-09-02 | 2013-12-18 | 浙江工业大学 | Ion exchange resin chromatography method and device without being promoted by external power |
| CN103446778B (en) * | 2013-09-02 | 2015-03-04 | 浙江工业大学 | Ion exchange resin chromatography method and device without being promoted by external power |
| CN104330492A (en) * | 2014-10-31 | 2015-02-04 | 华南理工大学 | Intelligent automatic switching multi-channel ion chromatographic column connecting device |
| CN106730999A (en) * | 2017-03-21 | 2017-05-31 | 天津科技大学 | One kind plus-minus pressure double-purpose operates desk-top chromatographic column |
| CN109669036A (en) * | 2019-02-28 | 2019-04-23 | 深圳市易瑞生物技术股份有限公司 | A kind of push type multichannel chromatographic apparatus |
| CN109669036B (en) * | 2019-02-28 | 2024-03-15 | 深圳市易瑞生物技术股份有限公司 | Push type multichannel chromatographic device |
| CN113713428A (en) * | 2021-06-30 | 2021-11-30 | 上海海路生物技术有限公司 | Chromatographic column extension device, system and method for packing column by using same |
| CN113713428B (en) * | 2021-06-30 | 2022-10-04 | 上海海路生物技术有限公司 | Chromatographic column extension device, system and method for packing column by using same |
| CN115738381A (en) * | 2022-11-08 | 2023-03-07 | 中国科学院过程工程研究所 | Multi-channel column chromatography device capable of automatically loading and eluting |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120523 Termination date: 20120914 |