CN115738381A - Multi-channel column chromatography device capable of automatically loading and eluting - Google Patents

Abstract

The invention relates to a multi-channel column chromatography device capable of automatic sample loading and elution. The device comprises a fixed holding layer, a sample collecting layer and an automatic sample loading and elution device. The device can be used separately, or it can be used as a whole with the automatic sample loading and elution device. When in use, the fixed clamping layer and the sample collection layer are provided with multiple channels, and multiple chromatographic columns can be used for operations at the same time, and the height of the fixed clamping layer and the position of the fixed fixture can be adjusted according to the type of chromatographic column. When using the automatic sample loading and elution device, pour the sample or eluent into the Martens flask, adjust the height of the automatic lifting platform, and control the liquid level in the chromatography column. The present invention and the automatic sample loading and elution method can realize slow, continuous and stable sample loading or elution, maintain the position of the liquid level in the chromatography column, and reduce the operating burden of the experimenter. The present invention is easy to use and can realize the simultaneous use of multiple channels. It is compatible with various types of chromatographic columns in the market, and can automatically load and elute, saving labor and time costs.

Description

A multi-channel column chromatography device capable of automatic sample loading and elution

technical field

The invention relates to the field of laboratory instruments, in particular to a multi-channel column chromatography device capable of automatic sample loading and elution, capable of using multiple channels for chromatography operations at the same time, quick installation and replacement of chromatography columns, and automatic sample loading The elution device is suitable for chromatography devices of various types of chromatography columns.

Background technique

Chromatography is a widely used separation and analysis method, which plays an important role in drug preparation, substance purification, and impurity purification. Chromatography column is the main body in chromatography technology, generally using glass tube or plexiglass tube. According to the amount of sample and the resolution of the target, the type of glass chromatography column required is also different, which is reflected in different column heights and diameters.

For the type of chromatographic column commonly used in the laboratory, the operator often uses an iron stand as a fixing frame for the chromatographic column to perform chromatographic experiments. Usually, one iron stand can fix at most two chromatography columns at the same time, which is inconvenient to use multiple chromatography columns at the same time, and at the same time, the clamping force of the clamp is too large, which easily damages the outer wall of the chromatography column.

In the pretreatment process of the sample to be tested using a chromatographic column, in order to obtain the target substance as comprehensively as possible, it is generally necessary to use a large amount of eluent for purification and elution, which takes up a lot of manpower and time, resulting in a waste of resources.

Therefore, it is necessary to develop a column chromatography device with stable placement, adjustable clamp force, no damage to the chromatography column, automatic sample loading and elution, and simultaneous use of multiple chromatography columns.

Contents of the invention

In order to solve the aforementioned technical problems, the purpose of the present invention is to provide a chromatographic device capable of automatic sample loading and elution, and suitable for simultaneously fixing various types of chromatographic columns.

Technical scheme of the present invention is as follows:

A multi-channel column chromatography device capable of automatic sample loading and elution is characterized in that the device includes an automatic sample loading and elution device and a multi-channel column chromatography device, and the two parts of the device can be used simultaneously or separately.

The aforementioned multi-channel column chromatography device is provided with multiple chromatography column fixing channels, which can simultaneously fix multiple chromatography columns. The aforementioned automatic sample loading and elution device is composed of a Martens flask and an automatic lifting device, and is arranged behind the corresponding position of each fixed channel.

The aforesaid multi-channel column chromatography device includes a base, a plate frame is provided on both sides of the base, and a positioning hole for fixing the clamping layer is provided in the middle of the plate frame; the fixed clamping layer includes a clamping layer fixing bar, and the spacer Metal clamps are provided; the fixed clamping layer is provided with upper and lower layers; a sample collection layer is provided between the base and the lower fixed clamping layer.

The frame body in the aforementioned multi-channel column chromatography device is composed of a stable base and two side plate frames.

The plate frame connected to the base in the aforementioned multi-channel column chromatography device is provided with different nut interfaces as required, and the position of the fixed clamping layer can be adjusted according to the type of chromatography column.

The fixed clamping layer connected to the plate frame in the aforementioned multi-channel column chromatography device is divided into two layers, which are respectively used to fix the upper end and the lower end of the chromatography column;

The fixture provided on the fixed clamping layer in the aforementioned multi-channel column chromatography device includes a two-piece metal clamp that can be opened and closed and a nut for fixing the metal clamp.

The fixing degree of the nut on the metal fixture in the aforementioned multi-channel column chromatography device can be adjusted according to the size of the chromatography column, and is applicable to various types of chromatography columns.

In the aforementioned multi-channel column chromatography device, a rubber strip is attached to the inside of the two-piece metal clip, which can increase the friction with the chromatography column and protect the outer wall of the chromatography column.

The sample layer used to place the sample collection tube in the aforementioned multi-channel column chromatography device is fixed to the plates on both sides, and the height is determined according to the size of the sample collection tube; the horizontal setting of the position of the collection tube is consistent with the position of the metal clamp in the fixed clamping layer; The hole diameter of the orifice of the sample collection tube is set according to the outer diameter of the collection tube used. Ensure that the collection tube is placed stably and all samples are collected without spillage.

The aforementioned automatic sample loading and elution device has a water outlet at the bottom of the Marble flask, which can be connected to the chromatographic column with a water delivery hose to realize automatic sample loading or elution.

The aforementioned automatic lifting device of the automatic sample loading and eluting device includes a fixed rod, a support plate and a fixed ring. The supporting plate and fixing ring are nested together with the lifting and fixing rod, which can freely adjust the height of the Martens flask and control the stable liquid level position in the chromatography column.

The aforementioned multi-channel column chromatography device capable of automatic loading and elution is used as follows:

Step 1: Adjust the positions of the two fixed clamping layers according to the column height of the chromatography column to ensure that the metal clamps can fix the upper and lower ends of the chromatography column.

Step 2: Fix the chromatography column. Use the upper and lower metal clamps to fix the chromatographic column respectively, and adjust the strength of the fixing nut according to the size of the chromatographic column to ensure that the chromatographic column does not slide up and down.

Step 3: Set up the automatic sample loading and elution device. According to the position of the liquid level of the chromatographic column, adjust the position of the Martens flask in the automatic sample loading and elution device by controlling the lifting platform, and use a water delivery hose to connect the water outlet of the Martens flask and the inlet of the chromatographic column to ensure automatic sample loading and elution During the process, uniform, continuous, and stable loading or elution.

Step 4: When there is no need to add a large amount of liquid, the automatic sample loading and elution device can be removed, and only the chromatography column holder can be used to fix the chromatography column for chromatography operation.

The beneficial effects of the present invention are as follows:

(1) A multi-channel column chromatography device capable of automatic sample loading and elution is provided with multiple sets of fixtures, which can meet the simultaneous execution of multiple chromatography processes.

(2) The metal clamp can be opened and closed freely, and the fixing force can be adjusted through the nut, which can meet the use of chromatography columns with different pore diameters.

(3) Rubber strips are attached to the inner side of the metal fixture to increase friction and protect the outer wall of the chromatography column.

(4) The position of the fixed clamping layer can be adjusted according to requirements, which can meet the use of chromatography columns with different column heights.

(5) The sample collection layer is provided with an orifice for placing the sample collection tube to fix the collection tube and prevent leakage, and can effectively collect chromatographic samples.

(6) The automatic sample loading and elution device can set the height of the liquid level according to the needs, realize automatic sample loading or elution, and reduce the operating burden of the experimenter.

(7) The automatic sample loading and elution device can realize uniform speed, continuous and stable sample loading or elution.

(8) The present invention can be disassembled and used. Only the multi-channel chromatography column holder is used to realize loading or elution of small-volume samples, and it can also be used in conjunction with an automatic sample loading and elution device to realize large-volume sample loading or elution.

Even if the effect is not specifically mentioned in the description of the present invention, if it belongs to the provisional effect that can be expected based on the technical features of the present invention, it should be treated equally as described in the description of the present invention.

Description of drawings

Fig. 1 is a schematic diagram of a multi-channel column chromatography device capable of automatic loading and elution of the present invention;

In the figure, 1-base, 2-plate frame, 3-holding layer fixing rod, 4-sample collection layer, 5-metal fixture, 6-automatic sample loading and elution device.

The accompanying drawings are merely reference examples provided to help understanding of the technical idea of the present invention, and the scope of rights of the present invention should not be limited thereto.

Detailed ways

The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments. But following embodiment only limits to explain the present invention, and protection scope of the present invention should comprise the whole content of claim, and by the narration of following embodiment, those skilled in the art can fully realize the whole content of claim of the present invention.

As shown in Figure 1, a multi-channel column chromatography device capable of automatic sample loading and elution includes a base 1, a plate frame 2 arranged on both sides of the base 1, and a positioning hole for fixing the clamping layer is provided in the middle of the plate frame; The fixed clamping layer includes clamping layer fixing rods 3, and metal clamps 5 are arranged at intervals on the clamping layer fixing rods 3; the fixed clamping layer is provided with upper and lower layers; a sample is arranged between the base 1 and the lower fixed clamping layer The collection layer 4; an automatic sample loading and elution device 6 is provided behind the corresponding position of each channel, and the automatic sample loading and elution device includes a Martens flask and an automatic lifting device.

The clamping layer fixing rod 3 is provided with a plurality of chromatographic column fixing channels, which can simultaneously fix a plurality of chromatographic columns. An automatic sample loading and eluting device 6 is arranged behind the corresponding position of each fixed channel, and the device is composed of a Martens flask and an automatic lifting device. The frame body capable of fixing the chromatographic column is composed of a stable base 1 and plate frames 2 on both sides.

The plate frame 2 connected with the base 1 is provided with different nut interfaces as required, and the height of the fixed clamping layer can be adjusted according to the column height of the chromatography column. The clamping layer fixing rod 3 connected with the plate frame 2 is divided into two layers, which are respectively used to fix the upper end and the lower end of the chromatographic column.

The metal clamp 5 provided on the fixed clamping layer includes a two-piece metal clamp that can be opened and closed and a nut for fixing the metal clamp. The nut on the metal fixture 5 can adjust the degree of fixation according to the size of the chromatographic column, and is used for various types of chromatographic columns. An anti-slip layer is set on the inner side of the two-piece metal clip, and the anti-slip layer is made of rubber to protect the glass chromatography column and increase friction.

The sample collection layer 4 used to place the sample collection tube is fixed to the plate frame 2 on both sides, and the height is determined according to the size of the sample collection tube; the horizontal setting of the collection tube position is consistent with the position of the metal clamp in the fixed clamping layer; the orifice of the sample collection tube is placed The pore size is set according to the outer diameter of the collection tube used. The surface of the sample collection layer used to place the sample collection tube is coated with anti-corrosion materials to prevent corrosion of the plate surface caused by sample spillage during chromatography. A water outlet is provided at the bottom of the Marble flask of the automatic sample loading and elution device 6, which is connected with the chromatography column by a water delivery hose to realize automatic sample loading or elution. The automatic lifting device of the automatic sample loading and eluting device 6 includes a lifting fixed rod, a support plate and a fixed ring; the supporting plate and the fixed ring can move freely relative to the lifting fixed rod.

The above-mentioned multi-channel column chromatography device capable of automatic loading and elution is used as follows:

Step 1: Adjust the positions of the two fixed clamping layers according to the column height of the chromatography column to ensure that the metal clamp 5 can fix the upper and lower ends of the chromatography column;

Step 2: Fix the chromatography column: use the upper and lower metal clamps 5 to fix the chromatography column respectively, and adjust the strength of the fixing nut according to the size of the chromatography column to ensure that the chromatography column does not slide up and down;

Step 3: Set up the automatic sample loading and elution device: according to the liquid level position of the chromatographic column, adjust the position of the Martens flask in the automatic sample loading and elution device by controlling the lifting platform, and use a water delivery hose to connect the water outlet of the Martens flask to the The inlet of the chromatographic column ensures uniform, continuous and stable sample loading and elution during the automatic sample loading and elution process;

Step 4: When there is no need to add a large amount of liquid, remove the automatic sample loading and elution device, and only use the chromatography column holder to fix the chromatography column for chromatography operation.

In one embodiment of the present invention, as shown in FIG. 1 , the present invention includes a shelf body capable of fixing chromatography columns. The shelf body includes a base 1, and plates 2 on both sides of the base 1. The plate frame 2 and the base 1 connect. The two sides of the plate frame 2 are perforated from top to bottom according to the specifications of the chromatographic column, as the position of the fixed layer and the sample collection layer, which can meet the needs of fixing chromatographic columns of multiple sizes.

The plate frame 2 and the upper and lower clamping layer fixing rods 3 are fixed on both sides in the form of nuts to form a double-layer fixed clamping layer (the interval height setting refers to the specification of the chromatography column).

The plate frame 2 and the sample collection layer 4 are fixed in the form of bonding, and the fixed position is set to be convenient for taking and placing the sample collection tube.

The metal fixture 5 is a sample fixing fixture. The sample fixing fixtures are respectively set from left to right and fixed with nuts. The sample fixing fixtures consist of two pieces of metal and adjustment nuts. When in use, place the chromatography column in the fixing fixture, use nuts to connect the two pieces of metal, and adjust the fixing degree of the nuts to ensure that the chromatography column does not slide up and down.

The orifice diameter of the collection tube placed on the sample collection layer 4 is set with reference to the outer diameter of the sample collection tube, and the center of the orifice is vertically set to the center of the sample fixing fixture.

According to the position of the fixed channel of the chromatography column, the corresponding automatic sample loading and eluting device 6 is set, and the Martens flask containing the sample or eluent to be processed is fixed on the support plate and lifting fixing rod of the automatic sample loading and eluting device 6 in the form of a fixed ring On the top, the height of the Marble flask can be adjusted freely according to the demand, and the position of the stable liquid level in the chromatography column can be controlled.

In addition, it should be understood that although this specification is described according to implementation modes, not each implementation mode only contains an independent technical solution, and this description in the specification is only for clarity, and those skilled in the art should take the specification as a whole , the technical solutions in the various embodiments can also be properly combined to form other implementations that can be understood by those skilled in the art.

Claims (14)

1. A multi-channel column chromatography device that can be automatically loaded and eluted is characterized in that the device includes an automatic sample loading and elution device and a multi-channel column chromatography device, an automatic sample loading and elution device and a multi-channel column layer The two parts of the device can be used simultaneously or separately. 2. A kind of multi-channel column chromatography device capable of automatic sample loading and elution according to claim 1, characterized in that, the multi-channel column chromatography device is provided with a plurality of chromatography column fixing channels, which can be fixed simultaneously Multiple chromatography columns. 3. a kind of multi-channel column chromatography device that can be automatically loaded and eluted according to claim 1 is characterized in that, the described automatic sample loading and elution device (6) is made up of a Marble flask and an automatic lifting device, and It is set behind the corresponding position of each fixed channel. 4. A multi-channel column chromatography device capable of automatic sample loading and elution according to claim 2, characterized in that, the multi-channel column chromatography device comprises a base (1), and base (1) both sides are provided with Plate frame (2), positioning holes for fixing the clamping layer are arranged in the middle of the plate frame (2); the fixed clamping layer includes clamping layer fixing rods (3), and the clamping layer fixing rods (3) are arranged at intervals A metal fixture (5); a fixed clamping layer is provided with upper and lower layers; a sample collection layer (4) is provided between the base (1) and the lower fixed clamping layer. 5. A kind of multi-channel column chromatography device capable of automatic sample loading and elution according to claim 4, characterized in that, the plate frame (2) connected to the base (1) is provided with different nut interfaces as required, and can Adjust the height of the fixed clamping layer according to the column height of the chromatography column. 6. A multi-channel column chromatography device capable of automatic sample loading and elution according to claim 4, characterized in that, the clamping layer fixing rod (3) connected to the plate frame (2) is divided into 2 layers , which are used to fix the upper and lower ends of the column, respectively. 7. A multi-channel column chromatography device capable of automatic sample loading and elution according to claim 4, characterized in that the metal clamp (5) set on the fixed clamping layer includes two pieces that can be opened and closed Type metal clips and nuts for fixing metal clips. 8. A multi-channel column chromatography device capable of automatic sample loading and elution according to claim 4, characterized in that the nut on the metal fixture can adjust the degree of fixation according to the size of the chromatography column, and is used for various types of layers Analysis column. 9. A multi-channel column chromatography device capable of automatic sample loading and elution according to claim 4, characterized in that an anti-slip layer is provided on the inner side of the two-piece metal clip, and the material of the anti-slip layer is rubber. 10. A kind of multi-channel column chromatography device capable of automatic loading and elution according to claim 4, characterized in that, the sample collection layer (4) and both sides plate holder (2) for placing the sample collection tube Fixed, the height is determined according to the size of the sample collection tube; the horizontal setting of the position of the collection tube is consistent with the position of the metal clamp (5) in the fixed clamping layer; the aperture of the orifice of the sample collection tube is set according to the outer diameter of the collection tube used. 11. A multi-channel column chromatography device capable of automatic sample loading and elution according to claim 1, characterized in that the surface of the sample collection layer for placing the sample collection tube is coated with an anti-corrosion material. 12. A kind of multi-channel column chromatography device capable of automatic sample loading and elution according to claim 3, characterized in that, the bottom of the Marble flask of the automatic sample loading and elution device (6) is provided with a water outlet, which is connected with the layer The analysis column is connected by a water hose to realize automatic sample loading or elution. 13. A kind of automatic loading and elution multi-channel column chromatography device according to claim 3, characterized in that, the automatic lifting device of the automatic sample loading and elution device (6) comprises a lifting fixing rod, a support plate and Fixed ring; the supporting plate and the fixed ring can move freely relative to the lifting fixed rod. 14. A multi-channel column chromatography device capable of automatic loading and elution according to claim 1, the method of use of which is as follows: Step 1: Adjust the positions of the two fixed clamping layers according to the column height of the chromatography column to ensure that the metal clamp (5) can fix the upper end and the lower end of the chromatography column; Step 2: Fix the chromatography column: use the upper and lower metal clamps (5) to fix the chromatography column respectively, and adjust the strength of the fixing nut according to the size of the chromatography column to ensure that the chromatography column does not slide up and down; Step 3: Set up the automatic sample loading and elution device (6): According to the position of the liquid level of the chromatography column, adjust the position of the Martens flask in the automatic sample loading and elution device by controlling the lifting platform, and use the water delivery hose to connect the Martens flask The water outlet and the inlet of the chromatographic column ensure uniform, continuous and stable sample loading and elution during the automatic sample loading and elution process; Step 4: When there is no need to add a large amount of liquid, remove the automatic sample loading and elution device (6), and only use the chromatography column holder to fix the chromatography column for chromatography operation.

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