CN109669036B - Push type multichannel chromatographic device - Google Patents
Push type multichannel chromatographic device Download PDFInfo
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- CN109669036B CN109669036B CN201910151070.XA CN201910151070A CN109669036B CN 109669036 B CN109669036 B CN 109669036B CN 201910151070 A CN201910151070 A CN 201910151070A CN 109669036 B CN109669036 B CN 109669036B
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- 238000006243 chemical reaction Methods 0.000 claims abstract description 153
- 230000007246 mechanism Effects 0.000 claims abstract description 121
- 238000001514 detection method Methods 0.000 claims abstract description 105
- 238000003825 pressing Methods 0.000 claims abstract description 48
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 15
- 239000011324 bead Substances 0.000 claims description 47
- 230000006835 compression Effects 0.000 claims description 28
- 238000007906 compression Methods 0.000 claims description 28
- 239000012528 membrane Substances 0.000 claims description 27
- 239000003550 marker Substances 0.000 claims description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 238000010521 absorption reaction Methods 0.000 claims description 13
- 239000000427 antigen Substances 0.000 claims description 12
- 102000036639 antigens Human genes 0.000 claims description 12
- 108091007433 antigens Proteins 0.000 claims description 12
- 238000003908 quality control method Methods 0.000 claims description 12
- 241000283707 Capra Species 0.000 claims description 10
- 239000002105 nanoparticle Substances 0.000 claims description 5
- 239000012474 protein marker Substances 0.000 claims description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 5
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 4
- 239000006249 magnetic particle Substances 0.000 claims description 3
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 206010011409 Cross infection Diseases 0.000 abstract description 2
- 206010029803 Nosocomial infection Diseases 0.000 abstract description 2
- 238000003317 immunochromatography Methods 0.000 description 12
- 239000000126 substance Substances 0.000 description 11
- 238000000034 method Methods 0.000 description 10
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 230000009471 action Effects 0.000 description 3
- 229910052782 aluminium Inorganic materials 0.000 description 3
- -1 aluminum ion Chemical class 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000005725 8-Hydroxyquinoline Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 229960003540 oxyquinoline Drugs 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- MCJGNVYPOGVAJF-UHFFFAOYSA-N quinolin-8-ol Chemical compound C1=CN=C2C(O)=CC=CC2=C1 MCJGNVYPOGVAJF-UHFFFAOYSA-N 0.000 description 2
- 239000013076 target substance Substances 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
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- 229920002301 cellulose acetate Polymers 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
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- 108020004707 nucleic acids Proteins 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
Abstract
The invention discloses a push type multi-channel chromatography device, comprising: a liquid storage tank; pressing the switching mechanism; the detection mechanism is sleeved outside the liquid storage tank and the pressing switching mechanism and comprises a first cylindrical body, and a plurality of test strips are arranged on the first cylindrical body; a second cylindrical body; the detection mechanism is slidably inserted into the second cylindrical body; the reaction cavity is arranged below the second cylindrical body and comprises at least two mutually independent reaction tanks; the flow guiding mechanism is arranged between the liquid storage tank and the reaction cavity and used for guiding the sample liquid flowing out of the liquid outlet of the liquid storage tank into each reaction tank. By arranging the flow guide mechanism, the sample liquid flowing out of the liquid outlet of the liquid storage tank can be synchronously and uniformly distributed into each reaction tank, so that the average distribution of the sample adding quantity is realized; the independent reaction of the chromatography in each reaction tank can meet the requirement of environmental sensitivity type test, can avoid cross infection and ensure the accuracy of detection results; the detection of various target detection objects can be realized by one sample adding.
Description
Technical Field
The invention relates to the technical field of biological detection, in particular to a push type multichannel chromatographic device.
Background
The chromatography detection is an important on-site rapid detection form in recent years, and is popular in the fields of clinical, food safety, environmental detection and the like due to convenience and rapidness. Currently, chromatography often requires the use of multiple test strips to perform multiple index assays on a single sample. For detection, it is common practice to place the sample fluid in a common chamber, then place a test strip, and read the results at a specific time. This approach is only suitable for chromatography in one-step and is not compatible with two-step methods. And the public cavity can pollute the detection process with high environmental requirements, and can not meet detection projects such as nucleic acid and the like. Other methods are that liquid in a common cavity is shunted into each independent chromatographic reaction cavity through independent flow channels, and then the flow channels are closed, so that the interval of chromatographic reaction processes is realized. However, this method is only suitable for chromatographic reactions in one-step processes, and is not possible in two-step processes.
Disclosure of Invention
In order to make up the defects of the prior art, the invention provides a push type multi-channel chromatography device.
The technical problems to be solved by the invention are realized by the following technical scheme:
a push-type multi-channel chromatography device comprising:
a liquid reservoir for containing a sample liquid; a liquid outlet is formed in the bottom end of the liquid storage tank;
the pressing switching mechanism is used for controlling the opening and closing of the liquid outlet by pressing and bouncing the pressing switching mechanism;
the detection mechanism is sleeved outside the liquid storage tank and the pressing switching mechanism and comprises a first cylindrical body with two open ends, and a plurality of test strips arranged in an array are arranged on the outer side wall of the first cylindrical body;
the upper end and the lower end of the second cylindrical body are both open; the detection mechanism is slidably inserted into the second cylindrical body, and moves up and down along the inner wall of the second cylindrical body, so that a sample pad of the test strip is contacted with or separated from sample liquid flowing into each reaction tank of the reaction cavity;
the reaction cavity is arranged below the second cylindrical body and comprises at least two mutually independent reaction tanks;
the flow guide mechanism is arranged between the liquid storage tank and the reaction cavity and used for guiding the sample liquid flowing out of the liquid outlet of the liquid storage tank into each reaction tank.
Further, press the shifter and include upper cover subassembly, depression bar, connecting rod, top pearl and compression spring, upper cover subassembly slidable mounting in the liquid storage tank upper end, top pearl is located liquid outlet department, depression bar one end with upper cover subassembly fixed connection, the depression bar other end passes the liquid outlet with top pearl links to each other, has cup jointed on the connecting rod compression spring, compression spring's top with the bottom butt of top pearl is used for with top pearl top is in on the liquid outlet.
Further, the upper cover assembly comprises an upper cover and a support, an upper end opening of the liquid storage tank is closed through the upper cover, the upper cover is hinged to the support, the support is installed in the liquid storage tank in a sliding mode, and the top bead is driven to open or close the liquid outlet.
Further, the flow guiding mechanism comprises a baffle plate fixedly arranged at the lower end of the second cylindrical body, one end of the connecting rod is fixed on the baffle plate, and a plurality of connecting through holes corresponding to the test paper strips and the reaction tank are formed in the baffle plate.
Further, the upper end of the reaction cavity is rotationally connected with the lower end of the second cylindrical body; the reaction cavity is provided with a first position and a second position relative to the second cylindrical body, and when the reaction cavity is positioned at the first position, the reaction groove and the connecting through hole are mutually aligned and communicated; when the reaction cavity is positioned at the second position, the reaction groove and the connecting through hole are staggered and the reaction groove is closed.
Further, the outer side wall of the first cylinder is provided with at least two accommodating grooves which are arranged in an array manner, the test strips are arranged in the corresponding accommodating grooves, and the sample pads of the test strips extend out of the first cylinder to form extension parts.
Further, the device also comprises a limiting mechanism; the detection mechanism is provided with a third position and a fourth position relative to the second cylindrical body, and when the detection mechanism is positioned at the third position, the test paper strip in the detection mechanism is separated from the sample liquid in the reaction tank; when the detection mechanism is positioned at the fourth position, a sample pad of the test strip in the detection mechanism passes through the connecting through hole to be in contact with the sample liquid in the reaction tank; the limiting mechanism is used for fixing the detection mechanism at the third position and the fourth position respectively.
Further, the limiting mechanism comprises at least two elastic protrusions arranged on the outer wall of the first cylinder body, and a first annular groove and a second annular groove which are arranged on the inner wall of the second cylinder body and are matched with the elastic protrusions, the first annular groove is arranged corresponding to the third position, and the second annular groove is arranged corresponding to the fourth position.
Further, the first cylinder and the second cylinder are transparent.
Further, the test strip is an immunochromatography test strip, the immunochromatography test strip comprises a bottom plate, a sample pad, a chromatographic membrane and a water absorption pad are sequentially lapped on one side of the bottom plate according to the chromatographic direction, and a detection line and a quality control line corresponding to a to-be-detected object are arranged on the chromatographic membrane; an antibody marker of an object to be detected or an antigen marker of the object to be detected is fixed in a reaction tank of the reaction cavity; the antibody label of the object to be detected is a block of an antibody-label conjugate of the object to be detected; the antigen marker of the object to be detected is a block of an antigen-carrier protein-marker conjugate of the object to be detected; the detection line is coated with an antibody of an object to be detected or an antigen-carrier protein conjugate of the object to be detected, and the quality control line is coated with a goat anti-rabbit IgG antibody or a goat anti-mouse IgG antibody; the marker is a colored particle or a fluorescent nanoparticle or a magnetic particle.
Further, the test strip is a chemical detection test strip, the chemical detection test strip comprises a bottom plate, a sample pad, a chromatographic membrane and a water absorption pad are sequentially lapped on one side of the bottom plate according to the chromatographic direction, chemical reagents are uniformly distributed on the chromatographic membrane, and the chemical reagents and an object to be detected in the sample liquid undergo specific chemical reaction and then undergo color change.
The invention has the following beneficial effects:
the invention is provided with the pressing switching mechanism, the top bead props against the liquid outlet at the bottom end of the liquid storage tank under the action force of the compression spring, and the sealing of the liquid storage tank is ensured; during detection, the upper cover assembly is pressed downwards by fingers, the upper cover assembly drives the top beads to move downwards, the top beads are pushed to further compress the compression springs, and a liquid outlet at the lower end of the liquid storage tank is opened, so that sample liquid flows out of the liquid storage tank; after the pressed finger is released, the top bead receives the acting force of the compression spring and returns to the initial position. By arranging the flow guide mechanism, the sample liquid flowing out of the liquid outlet of the liquid storage tank can be synchronously and uniformly distributed into each reaction tank, so that the average distribution of the sample adding quantity is realized, and the device is convenient to carry and easy and convenient to operate; the independent reaction of the chromatography in each reaction tank can meet the requirement of environmental sensitivity type test, can avoid cross infection and ensure the accuracy of detection results; the detection of multiple target detection objects can be realized by one sample adding, the problems of uneven distribution and difficult control of distribution of sample liquid in combined detection are solved, multiple detection can be simultaneously and stably carried out, the high-flux detection is realized, the workload of detection personnel is effectively reduced, and the detection efficiency is improved.
The detection mechanism of the invention can be inserted into the second cylinder body in a sliding way so as to slide in the second cylinder body along the vertical direction, so that the sample pad of the test strip is contacted with or separated from the sample liquid flowing into each reaction tank of the reaction cavity. Through the arrangement, the detection of the test strip by the two-step method can be realized, and if the sample liquid in the reaction tank needs to be uniformly mixed, the reaction cavity can be manually shaken, the test strip cannot be polluted, the adjacent reaction holes cannot be interfered, and the method has the characteristics of high sensitivity, high specificity, simplicity in use, low cost and the like.
The detection by the chromatographic device is carried out in a relatively closed system, so that the sample pollution and the possibility of detecting pollution are reduced to a certain extent, the detection stability is further improved, and the hidden danger of manual operation errors is also reduced.
According to the invention, according to the actual detection requirements, test strips for detecting different substances can be selected to be combined with the reaction tank, so that different requirements can be met.
According to the invention, after the test strips are detected, the test strips are uniformly distributed, and can be conveniently and accurately read when a reader reads the test strips, so that the test strips do not need to be aligned one by one, and the error is reduced.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a block diagram of the detection mechanism of the present invention;
FIG. 3 is a structural view of the reaction chamber of the present invention.
In the figure: 1. a liquid storage tank 2, an upper cover 3, a bracket 4, a compression bar 5, a connecting rod 6, a top bead 7, a compression spring 8 and a baffle plate, 9, a first cylinder body, 10, a second cylinder body, 11, a reaction cavity, 12, a reaction tank, 13 and a test strip.
Detailed Description
A push type multi-channel chromatography device comprises a liquid storage tank, a push switching mechanism, a detection mechanism, a second cylindrical body, a reaction cavity and a diversion mechanism.
The liquid storage tank is used for containing sample liquid; the bottom of the liquid storage tank is provided with a liquid outlet. Preferably, the liquid outlet is arranged in the middle of the bottom end of the liquid storage tank.
Preferably, the number of the liquid outlets is one.
The opening and closing of the liquid outlet are controlled by the pressing and bouncing of the pressing switching mechanism.
Preferably, the pressing switching mechanism comprises an upper cover assembly, a pressing rod, a connecting rod, a top bead and a compression spring, wherein the upper cover assembly is slidably arranged at the upper end of the liquid storage tank, the top bead is arranged at the liquid outlet, one end of the pressing rod is fixedly connected with the upper cover assembly, the other end of the pressing rod penetrates through the liquid outlet and is connected with the top bead, the compression spring is sleeved on the connecting rod, and the top end of the compression spring is abutted to the bottom of the top bead and used for propping the top bead against the liquid outlet.
The upper cover assembly comprises an upper cover and a support, an upper end opening of the liquid storage tank is closed through the upper cover, the upper cover is hinged to the support, the support is installed in the liquid storage tank in a sliding mode, and the top bead is driven to open or close the liquid outlet.
The top bead may take the form of a regular sphere. The diameter of the top bead is larger than that of the liquid outlet, and the diameter of the compression spring is smaller than that of the top bead.
The invention is provided with the pressing switching mechanism, and the top bead props against the liquid outlet at the bottom end of the liquid storage tank under the action force of the compression spring, so that the top bead closes the liquid outlet, and the liquid storage tank is ensured to be closed; during detection, the upper cover assembly is pressed downwards by fingers, the upper cover assembly drives the top beads to move downwards, the top beads are pushed to further compress the compression springs, and a liquid outlet at the lower end of the liquid storage tank is opened, so that sample liquid flows out of the liquid storage tank; after the pressed finger is released, the top bead receives the acting force of the compression spring and returns to the liquid outlet at the bottom end of the top bead liquid storage tank at the initial position.
The detection mechanism is sleeved on the outer side of the liquid storage tank and the pressing switching mechanism and comprises a first cylindrical body with two ends open, and a plurality of test strips arranged in an array are arranged on the outer side wall of the first cylindrical body.
In the present invention, the manner in which the test strip is disposed on the first cylindrical body is not particularly limited as long as the test strip is easily fixed and taken out. Preferably, the outer side wall of the first cylinder is provided with at least two accommodating grooves arranged in an annular array, the test strips are arranged in the corresponding accommodating grooves, and the sample pads of the test strips extend out of the first cylinder to form an extending part, but the test strips are not limited to the extending part.
The upper end and the lower end of the second cylindrical body are both open; the detection mechanism is slidably inserted in the second cylinder, and moves up and down along the inner wall of the second cylinder, so that the sample pad of the test strip is contacted with or separated from the sample liquid flowing into each reaction tank of the reaction cavity.
The reaction cavity is arranged below the second cylindrical body and comprises at least two mutually independent reaction tanks, and the reaction tanks are distributed in an annular array.
The upper end of the reaction cavity is rotationally connected with the lower end of the second cylindrical body; the reaction cavity is provided with a first position and a second position relative to the second cylindrical body, and when the reaction cavity is positioned at the first position, the reaction groove and the connecting through hole are mutually aligned and communicated; when the reaction cavity is positioned at the second position, the reaction groove and the connecting through hole are staggered and the reaction groove is closed.
In the present invention, the manner of the rotational connection is not particularly limited, and may be implemented in various manners conventional in the art, and preferably, the reaction chamber and the second cylinder are rotationally connected by screw threads. Specifically, an external thread is arranged on the outer side wall of the second cylindrical body, and an internal thread matched with the external thread is arranged at the upper end of the reaction cavity.
The flow guide mechanism is arranged between the liquid storage tank and the reaction cavity and is used for guiding the sample liquid flowing out of the liquid outlet of the liquid storage tank into each reaction tank.
To achieve the above object, various diversion mechanisms known in the art may be employed. Preferably, the flow guiding mechanism comprises a baffle plate fixedly arranged at the lower end of the second cylindrical body, one end of the connecting rod is fixed on the baffle plate, a plurality of connecting through holes corresponding to the test paper strips and the reaction tank are arranged on the baffle plate, and the connecting through holes are annularly arranged on the baffle plate.
According to the invention, the plurality of connecting through holes are formed in the partition plate, when the connecting through holes are aligned and communicated with the reaction tanks, sample liquid flowing out of the liquid outlet of the liquid storage tank enters each reaction tank through the connecting through holes, and the sample pad of the test strip can also penetrate through the connecting through holes to carry out chromatographic reaction with the sample liquid in the reaction tank.
At this time, the liquid storage tank and the detection mechanism are both arranged in a cavity surrounded by the second cylindrical body and the partition plate.
The device also comprises a limiting mechanism; the detection mechanism is provided with a third position and a fourth position relative to the second cylindrical body, and when the detection mechanism is positioned at the third position, the test paper strip in the detection mechanism is separated from the sample liquid in the reaction tank; when the detection mechanism is positioned at the fourth position, a sample pad of the test strip in the detection mechanism passes through the connecting through hole to be in contact with the sample liquid in the reaction tank; the limiting mechanism is used for fixing the detection mechanism at the third position and the fourth position respectively.
When the test strip is in actual use, the first cylinder body slides up and down along the second cylinder body, the limiting mechanism plays a role in limiting the displacement stroke of the first cylinder body, and when the first cylinder body slides downwards to a third position, the test strip on the first cylinder body is completely arranged in the second cylinder body and is not contacted with the sample liquid in the reaction tank; when the first cylinder is pressed down to the fourth position, the first cylinder is proved to move to the lower limit, and the sample pad of the test strip passes through the connecting through hole to be in contact with the sample liquid in the reaction tank, so that the moving position precision of the first cylinder is ensured, and the test strip on the first cylinder cannot be excessively pushed into the reaction tank to cause the break of the test strip.
In order to achieve the above object, various limit mechanisms known in the prior art may be employed, and preferably, the limit mechanism includes at least two elastic protrusions provided on the outer wall of the first cylinder, and a first annular groove and a second annular groove provided on the inner wall of the second cylinder, the first annular groove being provided corresponding to the third position, and the second annular groove being provided corresponding to the fourth position.
For the convenience of observation, the first cylinder and the second cylinder are transparent.
The chromatographic device can be used for chemical detection, the test strip is a chemical detection test strip, the chemical detection test strip comprises a bottom plate, a sample pad, a chromatographic membrane and a water absorption pad are sequentially lapped on one side of the bottom plate according to the chromatographic direction, chemical reagents are uniformly distributed on the chromatographic membrane, and the chemical reagents and an object to be detected in the sample liquid undergo specific chemical reaction and then undergo color change.
For example, when the sample liquid is the aluminum ion, 8-hydroxy-quinoline is uniformly distributed on the chromatographic film, and when the sample pad in the chemical detection test strip is contacted with the sample liquid to carry out chromatographic reaction, if the sample liquid contains the aluminum ion, the aluminum ion has high-specificity chemical reaction with the 8-hydroxy-quinoline on the chromatographic film through chromatographic action, so that the chromatographic film is changed from colorless to yellow, thereby achieving the purpose of rapid detection.
The chromatographic device can also be used for immunodetection, and the test strip is an immunochromatography test strip.
The chromatographic device can be used for one-step immunochromatography and two-step immunochromatography when being used for immunodetection.
When the chromatographic device is used for immunochromatography of a one-step method, the test strip comprises a bottom plate, a sample pad, a binding pad, a chromatographic membrane and a water absorption pad are sequentially lapped on one side of the bottom plate according to the chromatographic direction, and an antibody marker of an object to be detected or an antigen marker of the object to be detected is fixed on the binding pad; and the chromatographic membrane is provided with a detection line and a quality control line which correspond to the to-be-detected object.
When the chromatographic device is used for two-step immunochromatography, the test strip comprises a bottom plate, a sample pad, a chromatographic membrane and a water absorption pad are sequentially lapped on one side of the bottom plate according to the chromatographic direction, and a detection line and a quality control line corresponding to a to-be-detected object are arranged on the chromatographic membrane. Specifically, the test strip comprises a bottom plate, a sample pad, a chromatographic membrane and a water absorption pad, wherein the sample pad is arranged on one side of the bottom plate, the water absorption pad is arranged on the other side of the bottom plate, the chromatographic membrane is arranged between the sample pad and the water absorption pad, the adjacent sample pad and the chromatographic membrane are in overlapped connection at the joint, the chromatographic membrane and the water absorption pad are in overlapped connection at the joint, a detection line and a quality control line are arranged in the middle of the chromatographic membrane, the detection line is close to the sample pad, and the sample pad of the test strip extends out of the first cylinder body to form an extending part; an antibody marker of an object to be detected or an antigen marker of the object to be detected is fixed in a reaction tank of the reaction cavity.
The immunochromatography of the two-step method sets the label conjugate coated in the existing binding pad in the reaction tank, so that the label conjugate can react with the sample liquid to be detected in the reaction tank more fully, the defects of incomplete reaction, low efficiency and the like on the binding pad in the traditional test strip are overcome, the label conjugate and the label conjugate can react thoroughly in a homogeneous system, and the sensitivity is improved.
The sample pad is a polyester film, glass fiber or filter paper with a sample filtering function.
The water absorbing pad is water absorbing paper; the chromatographic membrane is a nitrocellulose membrane or a cellulose acetate membrane.
The label is a substance that can be used to identify and detect the collected signal, such as a colored particle, e.g., colloidal gold, but is not limited thereto, and may be, for example, latex, fluorescent nanoparticle, quantum dot, or up-conversion luminescent substance, etc. The invention not only can meet the requirements of rapid qualitative detection, but also can judge the validity of the sample result and quantitatively analyze the sample.
The antibody label of the object to be detected is a block of an antibody-label conjugate of the object to be detected; the antigen marker of the object to be detected is a block of an antigen-carrier protein-marker conjugate of the object to be detected; the detection line is coated with an antibody of an object to be detected or an antigen-carrier protein conjugate of the object to be detected, and the quality control line is coated with a goat anti-rabbit IgG antibody or a goat anti-mouse IgG antibody.
In the present invention, the number of the accommodating grooves, the connecting through holes and the reaction grooves is the same, and can be set according to actual needs, preferably 2 to 8, more preferably 4 to 6.
When two-step immunochromatography is adopted, the detection method of the invention comprises the following steps: (1) Processing the sample to obtain a sample liquid, and adding the sample liquid into a liquid storage tank; (2) Pressing down the upper cover assembly by fingers, driving the top bead to move downwards by the upper cover assembly, pushing the top bead to further compress the compression spring, opening a liquid outlet at the lower end of the liquid storage tank, and allowing sample liquid to flow out of the liquid storage tank and enter each reaction tank in the reaction cavity through the flow guide mechanism; the chromatographic device is shaken, an object to be detected in the sample liquid reacts with an antibody marker of the object to be detected or an antigen marker of the object to be detected fixed in the reaction tank, after the reaction is finished, the assembled detection mechanism slides downwards, so that a test strip penetrates through a baffle plate in the flow guide mechanism and is inserted into the reaction tank, a sample pad of the test strip contacts with the sample liquid in the detection channel to carry out immunochromatography reaction, the intensity of signals of each detection line on the chromatographic membrane is observed by naked eyes or an instrument is scanned, the number of corresponding target substances in the sample is determined, and the content of the corresponding target substances in the weaker signals is higher.
The present invention will be described in detail with reference to the following examples, which are only preferred embodiments of the present invention and are not limiting thereof.
Example 1
A push-type multi-channel chromatography device comprising:
a liquid reservoir for containing a sample liquid; a liquid outlet is formed in the bottom end of the liquid storage tank;
the pressing switching mechanism is used for controlling the opening and closing of the liquid outlet by pressing and bouncing the pressing switching mechanism; the pressing switching mechanism comprises an upper cover assembly, a pressing rod, a connecting rod, a top bead and a compression spring, wherein the upper cover assembly is arranged at the upper end of the liquid storage tank in a sliding mode, the top bead is arranged at the liquid outlet, one end of the pressing rod is fixedly connected with the upper cover assembly, the other end of the pressing rod penetrates through the liquid outlet to be connected with the top bead, the compression spring is sleeved on the connecting rod, and the top end of the compression spring is abutted to the bottom of the top bead to prop the top bead on the liquid outlet; the upper cover assembly comprises an upper cover and a bracket, an upper end opening of the liquid storage tank is closed through the upper cover, the upper cover is hinged to the bracket, and the bracket is arranged in the liquid storage tank in a sliding manner and drives the top bead to open or close the liquid outlet;
the detection mechanism is sleeved on the outer sides of the liquid storage tank and the pressing switching mechanism and comprises a first cylindrical body with two open ends, 4 accommodating grooves which are arranged in an array are formed in the outer side wall of the first cylindrical body, test strips are arranged in the corresponding accommodating grooves, and sample pads of the test strips extend out of the first cylindrical body to form extension parts; the test strip is an immunochromatography test strip, the immunochromatography test strip comprises a bottom plate, a sample pad, a chromatographic membrane and a water absorption pad are sequentially lapped on one side of the bottom plate according to the chromatographic direction, and a detection line and a quality control line corresponding to a to-be-detected object are arranged on the chromatographic membrane; an antibody marker of an object to be detected or an antigen marker of the object to be detected is fixed in a reaction tank of the reaction cavity; the antibody label of the object to be detected is a block of an antibody-label conjugate of the object to be detected; the antigen marker of the object to be detected is a block of an antigen-carrier protein-marker conjugate of the object to be detected; the detection line is coated with an antibody of an object to be detected or an antigen-carrier protein conjugate of the object to be detected, and the quality control line is coated with a goat anti-rabbit IgG antibody or a goat anti-mouse IgG antibody; the marker is colored particles or fluorescent nano particles or magnetic particles;
the upper end and the lower end of the second cylindrical body are both open; the detection mechanism is slidably inserted into the second cylindrical body, and moves up and down along the inner wall of the second cylindrical body, so that a sample pad of the test strip is contacted with or separated from sample liquid flowing into each reaction tank of the reaction cavity;
the reaction cavity is arranged below the second cylindrical body and comprises 4 mutually independent reaction tanks which are distributed in an annular array; the upper end of the reaction cavity is rotationally connected with the lower end of the second cylindrical body; the reaction cavity is provided with a first position and a second position relative to the second cylindrical body, and when the reaction cavity is positioned at the first position, the reaction groove and the connecting through hole are mutually aligned and communicated; when the reaction cavity is positioned at the second position, the reaction groove and the connecting through hole are staggered and the reaction groove is closed.
The flow guide mechanism is arranged between the liquid storage tank and the reaction cavity and used for guiding the sample liquid flowing out of the liquid outlet of the liquid storage tank into each reaction tank; the flow guide mechanism comprises a baffle plate fixedly arranged at the lower end of the second cylindrical body, one end of the connecting rod is fixed on the baffle plate, and the baffle plate is provided with 4 connecting through holes which are distributed in an annular array and correspond to the test strips and the reaction tank;
a limiting mechanism; the detection mechanism is provided with a third position and a fourth position relative to the second cylindrical body, and when the detection mechanism is positioned at the third position, the test paper strip in the detection mechanism is separated from the sample liquid in the reaction tank; when the detection mechanism is positioned at the fourth position, a sample pad of the test strip in the detection mechanism passes through the connecting through hole to be in contact with the sample liquid in the reaction tank; the limiting mechanism is used for fixing the detection mechanism at the third position and the fourth position respectively; the limiting mechanism comprises at least two elastic bulges arranged on the outer wall of the first cylinder body, and a first annular groove and a second annular groove which are arranged on the inner wall of the second cylinder body and are matched with the elastic bulges, wherein the first annular groove is arranged corresponding to the third position, and the second annular groove is arranged corresponding to the fourth position.
The first cylinder and the second cylinder are transparent.
Example 2
A push-type multi-channel chromatography device comprising:
a liquid reservoir for containing a sample liquid; a liquid outlet is formed in the bottom end of the liquid storage tank;
the pressing switching mechanism is used for controlling the opening and closing of the liquid outlet by pressing and bouncing the pressing switching mechanism; the pressing switching mechanism comprises an upper cover assembly, a pressing rod, a connecting rod, a top bead and a compression spring, wherein the upper cover assembly is arranged at the upper end of the liquid storage tank in a sliding mode, the top bead is arranged at the liquid outlet, one end of the pressing rod is fixedly connected with the upper cover assembly, the other end of the pressing rod penetrates through the liquid outlet to be connected with the top bead, the compression spring is sleeved on the connecting rod, and the top end of the compression spring is abutted to the bottom of the top bead to prop the top bead on the liquid outlet; the upper cover assembly comprises an upper cover and a bracket, an upper end opening of the liquid storage tank is closed through the upper cover, the upper cover is hinged to the bracket, and the bracket is arranged in the liquid storage tank in a sliding manner and drives the top bead to open or close the liquid outlet;
the detection mechanism is sleeved on the outer sides of the liquid storage tank and the pressing switching mechanism and comprises a first cylindrical body with two open ends, 8 accommodating grooves which are arranged in an array are formed in the outer side wall of the first cylindrical body, test strips are arranged in the corresponding accommodating grooves, and sample pads of the test strips extend out of the first cylindrical body to form extension parts; the test strip is an immunochromatographic test strip, the immunochromatographic test strip comprises a bottom plate, a sample pad, a binding pad, a chromatographic membrane and a water absorption pad are sequentially lapped on one side of the bottom plate according to the chromatographic direction, and an antibody marker of an object to be tested or an antigen marker of the object to be tested is fixed on the binding pad; the chromatographic membrane is provided with a detection line and a quality control line which correspond to the to-be-detected object; the antibody label of the object to be detected is a block of an antibody-label conjugate of the object to be detected; the antigen marker of the object to be detected is a block of an antigen-carrier protein-marker conjugate of the object to be detected; the detection line is coated with an antibody of an object to be detected or an antigen-carrier protein conjugate of the object to be detected, and the quality control line is coated with a goat anti-rabbit IgG antibody or a goat anti-mouse IgG antibody; the marker is colloidal gold or fluorescent nano particles;
the upper end and the lower end of the second cylindrical body are both open; the detection mechanism is slidably inserted into the second cylindrical body, and moves up and down along the inner wall of the second cylindrical body, so that a sample pad of the test strip is contacted with or separated from sample liquid flowing into each reaction tank of the reaction cavity;
the reaction cavity is arranged below the second cylindrical body and comprises 8 mutually independent reaction tanks which are distributed in an annular array; the upper end of the reaction cavity is rotationally connected with the lower end of the second cylindrical body; the reaction cavity is provided with a first position and a second position relative to the second cylindrical body, and when the reaction cavity is positioned at the first position, the reaction groove and the connecting through hole are mutually aligned and communicated; when the reaction cavity is positioned at the second position, the reaction groove and the connecting through hole are staggered and the reaction groove is closed.
The flow guide mechanism is arranged between the liquid storage tank and the reaction cavity and used for guiding the sample liquid flowing out of the liquid outlet of the liquid storage tank into each reaction tank; the flow guide mechanism comprises a baffle plate fixedly arranged at the lower end of the second cylindrical body, one end of the connecting rod is fixed on the baffle plate, and the baffle plate is provided with 8 connecting through holes which are distributed in an annular array and correspond to the test strip and the reaction tank;
a limiting mechanism; the detection mechanism is provided with a third position and a fourth position relative to the second cylindrical body, and when the detection mechanism is positioned at the third position, the test paper strip in the detection mechanism is separated from the sample liquid in the reaction tank; when the detection mechanism is positioned at the fourth position, a sample pad of the test strip in the detection mechanism passes through the connecting through hole to be in contact with the sample liquid in the reaction tank; the limiting mechanism is used for fixing the detection mechanism at the third position and the fourth position respectively; the limiting mechanism comprises at least two elastic bulges arranged on the outer wall of the first cylinder body, and a first annular groove and a second annular groove which are arranged on the inner wall of the second cylinder body and are matched with the elastic bulges, wherein the first annular groove is arranged corresponding to the third position, and the second annular groove is arranged corresponding to the fourth position.
The first cylinder and the second cylinder are transparent.
Example 3
A push-type multi-channel chromatography device comprising:
a liquid reservoir for containing a sample liquid; a liquid outlet is formed in the bottom end of the liquid storage tank;
the pressing switching mechanism is used for controlling the opening and closing of the liquid outlet by pressing and bouncing the pressing switching mechanism; the pressing switching mechanism comprises an upper cover assembly, a pressing rod, a connecting rod, a top bead and a compression spring, wherein the upper cover assembly is arranged at the upper end of the liquid storage tank in a sliding mode, the top bead is arranged at the liquid outlet, one end of the pressing rod is fixedly connected with the upper cover assembly, the other end of the pressing rod penetrates through the liquid outlet to be connected with the top bead, the compression spring is sleeved on the connecting rod, and the top end of the compression spring is abutted to the bottom of the top bead to prop the top bead on the liquid outlet; the upper cover assembly comprises an upper cover and a bracket, an upper end opening of the liquid storage tank is closed through the upper cover, the upper cover is hinged to the bracket, and the bracket is arranged in the liquid storage tank in a sliding manner and drives the top bead to open or close the liquid outlet;
the detection mechanism is sleeved on the outer sides of the liquid storage tank and the pressing switching mechanism and comprises a first cylindrical body with two open ends, 8 accommodating grooves which are arranged in an array are formed in the outer side wall of the first cylindrical body, test strips are arranged in the corresponding accommodating grooves, and sample pads of the test strips extend out of the first cylindrical body to form extension parts; the test strip is a chemical detection test strip, the chemical detection test strip comprises a bottom plate, a sample pad, a chromatographic membrane and a water absorption pad are sequentially lapped on one side of the bottom plate according to the chromatographic direction, chemical reagents are uniformly distributed on the chromatographic membrane, and the chemical reagents and an object to be detected in the sample liquid undergo specific chemical reaction and then undergo color change;
the upper end and the lower end of the second cylindrical body are both open; the detection mechanism is slidably inserted into the second cylindrical body, and moves up and down along the inner wall of the second cylindrical body, so that a sample pad of the test strip is contacted with or separated from sample liquid flowing into each reaction tank of the reaction cavity;
the reaction cavity is arranged below the second cylindrical body and comprises 8 mutually independent reaction tanks which are distributed in an annular array; the upper end of the reaction cavity is rotationally connected with the lower end of the second cylindrical body; the reaction cavity is provided with a first position and a second position relative to the second cylindrical body, and when the reaction cavity is positioned at the first position, the reaction groove and the connecting through hole are mutually aligned and communicated; when the reaction cavity is positioned at the second position, the reaction groove and the connecting through hole are staggered and the reaction groove is closed.
The flow guide mechanism is arranged between the liquid storage tank and the reaction cavity and used for guiding the sample liquid flowing out of the liquid outlet of the liquid storage tank into each reaction tank; the flow guide mechanism comprises a baffle plate fixedly arranged at the lower end of the second cylindrical body, one end of the connecting rod is fixed on the baffle plate, and the baffle plate is provided with 8 connecting through holes which are distributed in an annular array and correspond to the test strip and the reaction tank;
a limiting mechanism; the detection mechanism is provided with a third position and a fourth position relative to the second cylindrical body, and when the detection mechanism is positioned at the third position, the test paper strip in the detection mechanism is separated from the sample liquid in the reaction tank; when the detection mechanism is positioned at the fourth position, a sample pad of the test strip in the detection mechanism passes through the connecting through hole to be in contact with the sample liquid in the reaction tank; the limiting mechanism is used for fixing the detection mechanism at the third position and the fourth position respectively; the limiting mechanism comprises at least two elastic bulges arranged on the outer wall of the first cylinder body, and a first annular groove and a second annular groove which are arranged on the inner wall of the second cylinder body and are matched with the elastic bulges, wherein the first annular groove is arranged corresponding to the third position, and the second annular groove is arranged corresponding to the fourth position.
The first cylinder and the second cylinder are transparent.
The above examples only show embodiments of the present invention, and the description thereof is more specific and detailed, but should not be construed as limiting the scope of the invention, but all technical solutions obtained by equivalent substitution or equivalent transformation shall fall within the scope of the invention.
Claims (5)
1. A push type multi-channel chromatography device, comprising:
a liquid reservoir for containing a sample liquid; a liquid outlet is formed in the bottom end of the liquid storage tank;
the pressing switching mechanism is used for controlling the opening and closing of the liquid outlet by pressing and bouncing the pressing switching mechanism;
the detection mechanism is sleeved outside the liquid storage tank and the pressing switching mechanism and comprises a first cylindrical body with two open ends, and a plurality of test strips arranged in an array are arranged on the outer side wall of the first cylindrical body;
the upper end and the lower end of the second cylindrical body are both open; the detection mechanism is slidably inserted into the second cylindrical body, and moves up and down along the inner wall of the second cylindrical body, so that a sample pad of the test strip is contacted with or separated from sample liquid flowing into each reaction tank of the reaction cavity;
the reaction cavity is arranged below the second cylindrical body and comprises at least two mutually independent reaction tanks;
the flow guide mechanism is arranged between the liquid storage tank and the reaction cavity and used for guiding the sample liquid flowing out of the liquid outlet of the liquid storage tank into each reaction tank;
the pressing switching mechanism comprises an upper cover assembly, a pressing rod, a connecting rod, a top bead and a compression spring, wherein the upper cover assembly is arranged at the upper end of the liquid storage tank in a sliding mode, the top bead is arranged at the liquid outlet, one end of the pressing rod is fixedly connected with the upper cover assembly, the other end of the pressing rod penetrates through the liquid outlet to be connected with the top bead, the compression spring is sleeved on the connecting rod, and the top end of the compression spring is abutted to the bottom of the top bead to prop the top bead on the liquid outlet; the upper cover assembly comprises an upper cover and a bracket, an upper end opening of the liquid storage tank is closed through the upper cover, the upper cover is hinged to the bracket, and the bracket is arranged in the liquid storage tank in a sliding manner and drives the top bead to open or close the liquid outlet;
the flow guide mechanism comprises a baffle plate fixedly arranged at the lower end of the second cylindrical body, one end of the connecting rod is fixed on the baffle plate, and a plurality of connecting through holes corresponding to the test paper strips and the reaction tank are formed in the baffle plate;
the upper end of the reaction cavity is rotationally connected with the lower end of the second cylindrical body; the reaction cavity is provided with a first position and a second position relative to the second cylindrical body, and when the reaction cavity is positioned at the first position, the reaction groove and the connecting through hole are mutually aligned and communicated; when the reaction cavity is positioned at the second position, the reaction groove and the connecting through hole are staggered and the reaction groove is closed;
the device also comprises a limiting mechanism; the detection mechanism is provided with a third position and a fourth position relative to the second cylindrical body, and when the detection mechanism is positioned at the third position, the test paper strip in the detection mechanism is separated from the sample liquid in the reaction tank; when the detection mechanism is positioned at the fourth position, a sample pad of the test strip in the detection mechanism passes through the connecting through hole to be in contact with the sample liquid in the reaction tank; the limiting mechanism is used for fixing the detection mechanism at the third position and the fourth position respectively.
2. The push type multi-channel chromatographic device according to claim 1, wherein the outer side wall of the first cylinder is provided with at least two accommodating grooves arranged in an array, the test strips are arranged in the corresponding accommodating grooves, and the sample pads of the test strips extend out of the first cylinder to form an extension part.
3. The push-type multi-channel chromatography device according to claim 1, wherein the limiting mechanism comprises at least two elastic protrusions arranged on the outer wall of the first cylinder, and a first annular groove and a second annular groove which are arranged on the inner wall of the second cylinder and are matched with the elastic protrusions, the first annular groove is arranged corresponding to the third position, and the second annular groove is arranged corresponding to the fourth position.
4. The push-type multi-channel chromatography device of claim 1, wherein the first cylinder and the second cylinder are transparent.
5. The push type multi-channel chromatographic device according to claim 1, wherein the test strip is an immunochromatographic test strip, the immunochromatographic test strip comprises a bottom plate, a sample pad, a chromatographic membrane and a water absorption pad are sequentially lapped on one side of the bottom plate according to a chromatographic direction, and a detection line and a quality control line corresponding to a to-be-detected are arranged on the chromatographic membrane; an antibody marker of an object to be detected or an antigen marker of the object to be detected is fixed in a reaction tank of the reaction cavity; the antibody label of the object to be detected is a block of an antibody-label conjugate of the object to be detected; the antigen marker of the object to be detected is a block of an antigen-carrier protein-marker conjugate of the object to be detected; the detection line is coated with an antibody of an object to be detected or an antigen-carrier protein conjugate of the object to be detected, and the quality control line is coated with a goat anti-rabbit IgG antibody or a goat anti-mouse IgG antibody; the marker is a colored particle or a fluorescent nanoparticle or a magnetic particle.
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