CN207502545U - A kind of immuno-chromatography detection device of Portable rural residue of veterinary drug - Google Patents
A kind of immuno-chromatography detection device of Portable rural residue of veterinary drug Download PDFInfo
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- CN207502545U CN207502545U CN201721681169.3U CN201721681169U CN207502545U CN 207502545 U CN207502545 U CN 207502545U CN 201721681169 U CN201721681169 U CN 201721681169U CN 207502545 U CN207502545 U CN 207502545U
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Abstract
The utility model discloses a kind of immuno-chromatography detection devices of Portable rural residue of veterinary drug, it includes sample area, the conversion zone on sample area and at least one detection remaining immuno-chromatographic test paper strip of agricultural and veterinary chemicals for placing agricultural product treatment fluid to be measured, the sample pad of the immuno-chromatographic test paper strip is contacted with the agricultural product treatment fluid to be measured in the sample area, and the conversion zone includes marker.The utility model finally realizes the high-throughput detection premised on live easy operation, i.e., primary sample-adding realizes the remaining synchronous detection of a variety of agricultural and veterinary chemicals, and operation is easier, cost is less expensive, sample dosage is few.Testing result can visually judge the analysis that corresponding instrument detection means is also coordinated to realize qualitative, quantitative or sxemiquantitative.
Description
Technical field
The utility model is related to technical field of biological more particularly to a kind of immunochromatographies of Portable rural residue of veterinary drug
Detection device.
Background technology
Important tool of the agricultural and veterinary chemicals as prevention pest and disease damage, has been increasingly becoming an important link of agricultural production.But
Some loopholes during for supervision and use, agricultural and veterinary chemicals residue problem have been increasingly becoming one of obstacle of modern agricultural development.
In recent years agricultural and veterinary chemicals residual excessive problem increasingly by society concern and great attention, agricultural and veterinary chemicals residual it is exceeded caused by
It endangers increasing, has seriously affected people's lives.Therefore, how quickly, it is sensitive, accurately detect agricultural product and its processing
Residual agricultural and veterinary chemicals in product, are the key that solve the above problems.
Agricultural and veterinary chemicals residue detection mainly has two major class methods at present：Chromatographic detection and fast detection method.Chromatographic detection
Because the exactness and accuracy of its monitoring data can remain law enforcement for agricultural and veterinary chemicals and provide solid reference.But it operates journey
Sequence is complicated, needs high-tech professional testing staff, and detecting instrument and expense are all fairly expensive, are unfavorable for popular.Quick detection
Method is easy to operate, does not need to expensive instrument, is suitable for the selective mechanisms of Site Detection and gross sample.It is applied in fast detection method
Most commonly used is the rapid detection card using enzyme inhibition, and principle is that cholinesterase can be catalyzed indophenols acetic acid esters (red) water
It solves as acetic acid and indophenols (blue), organophosphor or carbamates agricultural and veterinary chemicals have inhibiting effect to cholinesterase, make catalysis, water
Solution, the process of discoloration change, and thus can determine whether out whether there is high dose organophosphor or carbamate chemicals for agriculture in sample
Presence.
But, on the one hand, agricultural and veterinary chemicals type is various, up to the present, nearly 2,000,000 tons of chemical pesticide annual output in the world,
There are about more than 1000 kinds of artificial-synthetic compounds to be used as the classes pesticide such as insecticide, fungicide, algicide, insecticide, defoliant, removes
Common Organophosphorus and carbamate pesticides class pesticide further includes Bravo, carbendazim, organochlorine, chrysanthemum ester etc., existing quick
Detection method can not effectively detect it；On the other hand, the quick detection card of existing agricultural and veterinary chemicals residual needs when in use
Multiple tools is coordinated to be sampled, whole process needs to carry a large amount of tools, is not suitable for growing area and checks at any time.
In view of the above-mentioned problems, start the remaining immunity test strip of agricultural and veterinary chemicals occur on the market, but on the one hand, it is existing to exempt from
Epidemic disease chromatograph test strip is made of 4 parts, i.e., has been passed sequentially through successively by sample pad, bonding pad, chromatographic film, water absorption pad
The bottom plate of adhesive is mutually lapped successively, is reacted and is not thorough on bonding pad, not exclusively, efficiency is low, and sensitivity is not high；It is another
Aspect, existing test strips are often single target detection, it is impossible to while the detection of multiple indexs is carried out to a sample, and it is to be checked
There may be a variety of agricultural and veterinary chemicals residual in the agricultural product of survey, existing immune detection is both needed to be repeated as many times a sample and operate,
The detection one by one of more targets can be completed, not only increases the lengthy and tedious time-consuming and laborious of monitoring in this way, be easy to cause the waste of sample, and
Easily there are manual errors, extend detection time, live poor operability.
Utility model content
In order to solve above-mentioned the deficiencies in the prior art, the utility model provides a kind of immune layer of Portable rural residue of veterinary drug
Analyse detection device.
Technical problem to be solved in the utility model is achieved by the following technical programs：
A kind of immuno-chromatography detection device of Portable rural residue of veterinary drug, including for placing agricultural product treatment fluid to be measured
Sample area, the conversion zone on sample area and at least one detection the remaining immuno-chromatographic test paper strip of agricultural and veterinary chemicals, it is described to exempt from
The sample pad of epidemic disease chromatograph test strip is contacted with the agricultural product treatment fluid to be measured in the sample area, and the conversion zone includes label
Object.
Further, including the different remaining immuno-chromatographic test paper strips of agricultural and veterinary chemicals of at least two detections.
Further, including housing cavity, the sample area is placed in the accommodating cavity bottom end, the immunity-chromatography test
Paper slip is set on the side wall of the accommodating cavity, and the accommodating cavity upper end is additionally provided with cover.
Further, the sample area is trench structure.
Further, the accommodating cavity and sample area integrated molding or the accommodating cavity and the sample
Area is detachably connected.
Further, the cross section of the accommodating cavity is polygon, circle or ellipse.
Further, the side wall of the accommodating cavity is provided with backboard, and the backboard has at least two locating grooves, institute
It is arranged in parallel to state locating groove, the immuno-chromatographic test paper strip is placed in corresponding locating groove, and the backboard is not using
The hard plastic item of water suction is made, and the backboard moves up and down in the accommodating cavity.
Further, the immuno-chromatographic test paper strip includes bottom plate, is taken successively by chromatography direction on the bottom plate side
Sample pad, chromatographic film, water absorption pad are connected to, the chromatographic film is equipped with detection line corresponding with agricultural and veterinary chemicals to be measured and Quality Control
Line.
Further, the conversion zone for agricultural and veterinary chemicals to be measured antigen-carrier albumen-marker conjugate block or
The block of the antibody-marker object conjugate of person's agricultural and veterinary chemicals to be measured, be coated in the detection line agricultural and veterinary chemicals to be measured antibody or
The antigen-carrier protein conjugate of person's agricultural and veterinary chemicals to be measured is coated with goat anti-rabbit igg antibody or sheep anti-mouse igg on the nature controlling line
Antibody.
Further, the chromatographic film is equipped with a plurality of detection line.
Further, the marker is colloidal gold or fluorescent nano particle.
Further, the accommodating cavity is upper is equipped with result display window, the accommodating cavity at the position of chromatographic film
On be additionally provided with information registration label, described information registration label on is printed with detection object information and result judge explanation.
The utility model has the advantages that：
（1）The utility model finally realizes the high-throughput detection premised on live easy operation, i.e., primary sample-adding is real
The existing remaining synchronous detection of a variety of agricultural and veterinary chemicals, operation is easier, cost is less expensive, sample dosage is few.Testing result can visually be sentenced
The disconnected analysis that corresponding instrument detection means can also be coordinated to realize qualitative, quantitative or sxemiquantitative.
（2）The detection carried out using the detection device of the utility model is carried out in the system of a relative closure,
Reduce sample contamination and the possibility of detection pollution to a certain extent, further improve the stability of detection, also reduce
The hidden danger of manual operation error.
（3）Compact is light, easy to carry, suitable for the field quick detection of agricultural and veterinary chemicals residual quantity, can meet scene
The needs of rapid screening or semiquantitative determination.
（4）Existing immuno-chromatographic test paper strip is made of 4 parts, i.e., successively by sample pad, bonding pad, chromatography
The bottom plate that film, water absorption pad pass sequentially through adhesive is mutually lapped successively, and the immuno-chromatographic test paper strip of the utility model
In be not bound with pad, marker conjugate coated in existing bonding pad is set to the agricultural production to be measured in sample area and sample area
Product extracting solution is more fully reacted, and is improved in traditional test strip reaction on bonding pad and is not thorough, not exclusively, efficiency is low etc.
Defect so that agricultural and veterinary chemicals to be measured are thoroughly reacted with marker conjugate in homogeneous system, improve sensitivity.
Description of the drawings
Fig. 1 is the partial perspective view of the utility model；
Fig. 2 is another partial perspective view of the utility model.
In figure：1st, sample area, 2, accommodating cavity, 3, backboard, 4, boss, 5, conversion zone, 6, locating groove.
Specific embodiment
Refering to Fig. 1-2, a kind of immuno-chromatography detection device of Portable rural residue of veterinary drug, including for placing agriculture to be measured
The sample area of product treatment liquid, the conversion zone on sample area and the different agricultural and veterinary chemicals of at least two detections being integrated in one
Remaining immuno-chromatographic test paper strip, the sample pad of the immuno-chromatographic test paper strip are handled with the agricultural product to be measured in the sample area
Liquid contacts, and the conversion zone includes marker.
The immuno-chromatography detection device of the utility model is preferably vertical structure, and the sample area is placed in the accommodating cavity
Bottom end, the immuno-chromatographic test paper strip are set on the side wall of the accommodating cavity, it is to be understood that the immunity-chromatography test
Paper slip can be set on the inner wall of the accommodating cavity, and the immuno-chromatographic test paper strip can also be set to the accommodating cavity
Outer wall on.In the utility model, the quantity of the immuno-chromatographic test paper strip is not particularly limited, those skilled in the art can
Flexibly to be set according to actually detected needs.In the utility model, the accommodating cavity upper end is provided with cover.
Preferably, the sample area is trench structure, but not limited to this.It is understood that any be suitable for putting
The structure for putting sample can operate with the utility model.
As a further improvement, the sample area includes being set to boss of the upper end for arc-shaped of sample district center
And the reactive tank being surrounded on around the boss, the sample pad of the immuno-chromatographic test paper strip pass through reactive tank and the sample area
Interior agricultural product treatment fluid to be measured contact, can be branched to the agricultural product treatment fluid to be measured poured into naturally by this set convex
In the reactive tank of platform surrounding, the usage amount of sample can be greatly decreased.
The reaction is placed in the reactive tank, and the conversion zone is antigen-carrier albumen-label of agricultural and veterinary chemicals to be measured
The block of the antibody-marker object conjugate of the block of object conjugate or agricultural and veterinary chemicals to be measured.
In the utility model, the accommodating cavity can be integrally formed with the sample area.
As a further improvement, the accommodating cavity can also be with the sample area and be detachably connected, and as an example, institute
It states and is detachably connected as connecting by snap, the sample pad of immuno-chromatographic test paper strip is exposed to except accommodating cavity, works as sample
When product area is connected with accommodating cavity by snap, sample pad is closed within accommodating cavity.
In the utility model, the shape of the accommodating cavity is not particularly limited, it is preferable that the horizontal stroke of the accommodating cavity
Section is polygon, circle or ellipse.
In the utility model, the accommodating cavity is preferably transparent.
In the utility model, the side wall of the accommodating cavity is provided with backboard, and there are the backboard at least two positioning to block
Slot, the locating groove is arranged in parallel, and the immuno-chromatographic test paper strip is placed in corresponding locating groove, and the backboard is adopted
It is made of the hard plastic item not absorbing water.In the utility model, the quantity of locating groove is not particularly limited, art technology
Personnel can flexibly be set according to actually detected needs, and as an example, the quantity of the locating groove can be 6,8
It is a, 10.
In the utility model, so backboard is detachably connected with accommodating cavity wall, the backboard can be described accommodating
It is moved up and down in cavity.
In the utility model, the immuno-chromatographic test paper strip include bottom plate, on the bottom plate side by chromatography direction according to
Secondary to be overlapped with sample pad, chromatographic film, water absorption pad, the chromatographic film is equipped with detection line corresponding with agricultural and veterinary chemicals to be measured and matter
Control line.Specifically, the middle part of bottom plate is chromatographic film, has detection line and a nature controlling line in chromatographic film, one end end of bottom plate is
Sample pad, other end end are water absorption pad；The both ends of chromatographic film mutually overlap connection with water absorption pad and sample pad respectively.The inspection
Survey line can set one, can also set a plurality of.
Wherein, block of the conversion zone for antigen-carrier albumen-marker conjugate of agricultural and veterinary chemicals to be measured, it is described
The antibody of agricultural and veterinary chemicals to be measured is coated in detection line, goat anti-rabbit igg antibody is coated on the nature controlling line or sheep anti-mouse igg resists
Body；Alternatively, the conversion zone is the block of the antibody-marker object conjugate of agricultural and veterinary chemicals to be measured, it is coated in the detection line
The antigen-carrier protein conjugate of agricultural and veterinary chemicals to be measured is coated with goat anti-rabbit igg antibody on the nature controlling line or sheep anti-mouse igg resists
Body.
Existing immuno-chromatographic test paper strip is made of 4 parts, i.e., successively by sample pad, bonding pad, chromatographic film,
The bottom plate that water absorption pad passes sequentially through adhesive is mutually lapped successively, and is not had in the immuno-chromatographic test paper strip of the utility model
There is bonding pad, marker conjugate coated in existing bonding pad is set to sample area carries with the agricultural product to be measured in sample area
Liquid is taken more fully to be reacted, reaction on bonding pad is improved in traditional test strip and is not thorough, not exclusively, the low grade of efficiency lacks
It falls into so that agricultural and veterinary chemicals to be measured are thoroughly reacted with marker conjugate in homogeneous system, improve sensitivity.
The agricultural and veterinary chemicals to be measured include organophosphor, carbamate, Bravo, carbendazim, organochlorine, chrysanthemum ester, tetracycline,
Terramycin, aureomycin, fortimicin, special step his woods, Cimaterol, salbutamol, Fino Tero, Ractopamine, chlorine third that
Woods, Clenbuterol, Tulobuterol, penbutolol, phenolethanolamine A, Pefloxacin, Ciprofloxacin, Enrofloxacin, Ofloxacin,
Enoxacin, pipemidic acid, acidum nalidixicum, cinoxacin, Norfloxacin, Danofloxacin, Lomefloxacin, Difloxacin, husky fluorine are husky
Star, Sparfloxacin, fanasil, sulphadiazine, sulphathiazole, sulfapryidine, sulfamethyldiazine, sulfanilamide (SN) two
Jia Ji oxazoles, 5-methoxysulfadiazine, sulfadimidine, ayerlucil, sulfamethoxypyridazine, Sulfamethoxazole, sulphur
Amine chlorine reaches piperazine, daimeton, sulfacetamide, sulfafurazole, sulfaphenazolum, sulfadimethoxine, sulphur
At least two in An quinoxalines, but not limited to this.
It is appreciated that the utility model can be a kind of immuno-chromatography detection device of high-throughput pesticide residue, Ke Yitong
The remaining quick detection of Shi Shixian Multiple Pesticides, the pesticide include organophosphor, carbamate, Bravo, carbendazim, organic
At least two in chlorine, chrysanthemum ester.The utility model can also be a kind of immuno-chromatography detection device of high-throughput residue of veterinary drug, can
To realize the quick detections of a variety of residues of veterinary drug simultaneously, the veterinary drug include tetracycline medication, beta receptor stimulant medicine,
Quinolone drugs, sulfa drugs, specifically, the veterinary drug include tetracycline, terramycin, aureomycin, fortimicin, special step
His woods, Cimaterol, salbutamol, Fino Tero, Ractopamine, Clorprenaline, Clenbuterol, Tulobuterol, spraying
Luo Er, phenolethanolamine A, Pefloxacin, Ciprofloxacin, Enrofloxacin, Ofloxacin, enoxacin, pipemidic acid, acidum nalidixicum, west
Promise sand star, Norfloxacin, Danofloxacin, Lomefloxacin, Difloxacin, Sarafloxacin, Sparfloxacin, sulfanilamide (SN) neighbour's dimethoxy are phonetic
Pyridine, sulphadiazine, sulphathiazole, sulfapryidine, sulfamethyldiazine, sulfanilamide (SN) Er Jia Ji oxazoles, 5-methoxysulfadiazine, sulphur
Amine diformazan pyrimidine, ayerlucil, sulfamethoxypyridazine, Sulfamethoxazole, sulfachlorpyridazine, daimeton, sulphur
At least two in amine vinegar acyl, sulfafurazole, sulfaphenazolum, sulfadimethoxine, sulfaquinoxaline.This reality
With it is novel can also be a kind of immuno-chromatography detection device of portable Portable rural residue of veterinary drug, can realize a variety of agricultures simultaneously
The quick detection of medicine and residue of veterinary drug.
In the utility model, the marker on the bonding pad is preferably colloidal gold, but not limited to this, as an example,
It can also be electroselenium, nano rare earth fluorescence complex, nano magnetic particle.The utility model can not only meet fast qualitative
Testing requirements, can also carry out Effective judgement and sample amounts to sample result and analyze.
The material that cavity and sample area are housed in the utility model, in the utility model is impermeable material, as an example,
Can be plastics, metal, glass etc., but not limited to this.
The accommodating cavity is upper to be equipped with result display window at the position of chromatographic film, and letter is additionally provided on the accommodating cavity
Breath registration label, described information, which is registered, is printed with detection object information and result judgement explanation on label.
The utility model application method is：
After agricultural product have extracted, agricultural product treatment fluid to be measured is put into sample area, agricultural product treatment fluid elder generation to be measured and sample
The conversion zone of area bottom carries out reaction 5s-30min, then will be pushed away under backboard so that the sample of the immuno-chromatographic test paper strip on backboard
Product pad is contacted with the agricultural product treatment fluid product to be measured in the sample area, after 5s-30min, visually observes or instrument scanning chromatographs
The power of each detection line signal on film, determines the number of respective objects substance in sample, and signal gets over respective objects in weak sample
The content of substance is also higher.
Embodiment described above only expresses the embodiment of the utility model, and description is more specific and detailed, but simultaneously
Cannot the limitation to the utility model patent range therefore be interpreted as, as long as the form institute using equivalent substitution or equivalent transformation
The technical solution of acquisition should all fall and be within the protection scope of the utility model.
Claims (10)
1. a kind of immuno-chromatography detection device of Portable rural residue of veterinary drug, which is characterized in that it includes placing agriculture to be measured
The sample area of product treatment liquid, the conversion zone on sample area and at least one detection remaining immunity-chromatography test of agricultural and veterinary chemicals
Paper slip, the sample pad of the immuno-chromatographic test paper strip are contacted with the agricultural product treatment fluid to be measured in the sample area, the reaction
Layer includes marker.
2. immuno-chromatography detection device as described in claim 1, which is characterized in that it includes the different agriculture beasts of at least two detections
The remaining immuno-chromatographic test paper strip of medicine.
3. immuno-chromatography detection device as claimed in claim 1 or 2, which is characterized in that it further includes accommodating cavity, the sample
Product area is placed in the accommodating cavity bottom end, and the immuno-chromatographic test paper strip is set on the side wall of the accommodating cavity, the appearance
It puts cavity upper end and is additionally provided with cover.
4. immuno-chromatography detection device as claimed in claim 3, which is characterized in that the sample area is trench structure, described
Accommodating cavity is integrally formed with the sample area or the accommodating cavity is detachably connected with the sample area.
5. immuno-chromatography detection device as claimed in claim 3, which is characterized in that the cross section of the accommodating cavity is polygon
Shape, circle or ellipse.
6. immuno-chromatography detection device as claimed in claim 3, which is characterized in that the side wall of the accommodating cavity is provided with the back of the body
Plate, the backboard have at least two locating grooves, and the locating groove is arranged in parallel, and the immuno-chromatographic test paper strip is put
In corresponding locating groove, the backboard is made of the hard plastic item not absorbing water, and the backboard is in the accommodating cavity
In move up and down.
7. immuno-chromatography detection device as claimed in claim 3, which is characterized in that the immuno-chromatographic test paper strip includes bottom
Plate is overlapped with sample pad, chromatographic film, water absorption pad successively on the bottom plate side by chromatography direction, and the chromatographic film is equipped with
Detection line corresponding with agricultural and veterinary chemicals to be measured and nature controlling line.
8. immuno-chromatography detection device as claimed in claim 7, which is characterized in that the conversion zone is the anti-of agricultural and veterinary chemicals to be measured
The block of the block of original-carrier protein-marker conjugate or the antibody-marker object conjugate of agricultural and veterinary chemicals to be measured, institute
State the antigen-carrier protein conjugate of the antibody that agricultural and veterinary chemicals to be measured are coated in detection line or agricultural and veterinary chemicals to be measured, the Quality Control
Goat anti-rabbit igg antibody or sheep anti-mouse igg antibody are coated on line.
9. immuno-chromatography detection device as claimed in claim 8, which is characterized in that the marker is received for colloidal gold or fluorescence
Rice grain, the chromatographic film are equipped with a plurality of detection line.
10. immuno-chromatography detection device as claimed in claim 7, which is characterized in that the accommodating cavity is upper in chromatographic film
Position at be equipped with result display window, information registration label is additionally provided on the accommodating cavity, is printed on described information registration label
Information and result brushed with detection object judge explanation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721681169.3U CN207502545U (en) | 2017-12-06 | 2017-12-06 | A kind of immuno-chromatography detection device of Portable rural residue of veterinary drug |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721681169.3U CN207502545U (en) | 2017-12-06 | 2017-12-06 | A kind of immuno-chromatography detection device of Portable rural residue of veterinary drug |
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| Publication Number | Publication Date |
|---|---|
| CN207502545U true CN207502545U (en) | 2018-06-15 |
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ID=62508292
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721681169.3U Active CN207502545U (en) | 2017-12-06 | 2017-12-06 | A kind of immuno-chromatography detection device of Portable rural residue of veterinary drug |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109669036A (en) * | 2019-02-28 | 2019-04-23 | 深圳市易瑞生物技术股份有限公司 | A kind of push type multichannel chromatographic apparatus |
-
2017
- 2017-12-06 CN CN201721681169.3U patent/CN207502545U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109669036A (en) * | 2019-02-28 | 2019-04-23 | 深圳市易瑞生物技术股份有限公司 | A kind of push type multichannel chromatographic apparatus |
| CN109669036B (en) * | 2019-02-28 | 2024-03-15 | 深圳市易瑞生物技术股份有限公司 | Push type multichannel chromatographic device |
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