CN104374723A - Pesticide residue detector - Google Patents

Pesticide residue detector Download PDF

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Publication number
CN104374723A
CN104374723A CN201410675349.5A CN201410675349A CN104374723A CN 104374723 A CN104374723 A CN 104374723A CN 201410675349 A CN201410675349 A CN 201410675349A CN 104374723 A CN104374723 A CN 104374723A
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CN
China
Prior art keywords
led light
light source
pesticide residue
chip microcomputer
instrument
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Pending
Application number
CN201410675349.5A
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Chinese (zh)
Inventor
林新光
袁士猛
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CHANGCHUN CHANGGUANG SIPO SPECTRUM TECHNOLOGY Co Ltd
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CHANGCHUN CHANGGUANG SIPO SPECTRUM TECHNOLOGY Co Ltd
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Application filed by CHANGCHUN CHANGGUANG SIPO SPECTRUM TECHNOLOGY Co Ltd filed Critical CHANGCHUN CHANGGUANG SIPO SPECTRUM TECHNOLOGY Co Ltd
Priority to CN201410675349.5A priority Critical patent/CN104374723A/en
Publication of CN104374723A publication Critical patent/CN104374723A/en
Pending legal-status Critical Current

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Abstract

The invention discloses a pesticide residue detector which relates to the field of detection of agricultural products, and solves the problems in the prior art. The detector comprises a master controller, an LED light source group, a micro printer, a liquid crystal touch display screen, a storage battery, a USB interface, a photoelectric sensor, a power interface and a detection device, wherein the master controller consists of a single chip microcomputer, and a storage device, an analog/digital (A/D) converter and a time controller which are electrically connected with the single chip microcomputer; the LED light source group, the micro printer, the liquid crystal touch display screen and the storage battery are respectively and electrically connected with the single chip microcomputer; the USB interface is electrically connected with the storage device; the photoelectric sensor is electrically connected with the A/D converter; the power interface is electrically connected with the storage battery; the detection device, the LED light source group and the photoelectric sensor are positioned on the same horizontal line; the LED light source group consists of multiple LED light sources and has the central wavelength of 412nm; the detection device consists of multiple detection channels; and the LED light sources correspond to the detection channels one by one. According to the pesticide residue detector disclosed by the invention, a keyboard and a display are fused into a whole by using the liquid crystal touch display screen, the deadweight of the detector is lightened, and the detector has the advantages of being convenient to carry, simple to operate, large in viewing angle, high in transmissivity and low in energy consumption, being used by non-professionals and the like.

Description

Instrument for detecting pesticide residue through
Technical field
The present invention relates to technical field of agricultural product detection, be specifically related to a kind of instrument for detecting pesticide residue through that can measure Organophosphorus and carbamate pesticides class pesticide concentration in agricultural product.
Background technology
China is a large agricultural country, the process of crop diseases and pest disaster problem mainly still depends on pesticide spraying, this just makes the use amount of China's agricultural chemicals far above other countries, agricultural chemicals is after being sprayed onto on crops, major part can transform and be dispersed in the middle of nature, but also have a small amount of residues of pesticides on crops, the long-term edible physical and mental health having the agricultural product meeting high risks people of residues of pesticides, and the use of China's agricultural chemicals is in the majority with Organophosphorus and carbamate pesticides class agricultural chemicals, therefore this type of persticide residue on agricultural product is detected and just seem particularly important.
The detection method of current existing residues of pesticides mainly contains chemical fast determining method, immunoassay, inhibiting AChE and In vivo detection method etc., these methods have complicated operation, costly, the feature such as the low and sense cycle of accuracy rate is long.(1) chemical fast determining method, mainly according to redox reaction, for the quick detection of organophosphorus pesticide, although this method is easy, also can be used for Site Detection, sensitivity is low, has limitation, and is subject to reducing substances interference.(2) immunoassay, it is a kind of analytical approach based on having specific recognition and tuberculosis reaction between antigen and antibody, mainly contain radiommunoassay and EIA enzyme immunoassay, that the most frequently used is enzyme-linked immuno assay (ELISA), and it is highly sensitive, high specificity, the feature such as simple and efficient to handle.(3) inhibiting AChE, study the residual detection technique of the most ripe, most widely used quick agriculture at present, mainly according to Organophosphorus and carbamate pesticides class agricultural chemicals, reaction is suppressed, for the mensuration to Organophosphorus and carbamate pesticides pesticide residue to the specificity of enzyme acetylcholine.(4) living body biological mainly contacts with there being the specimen material of remains of pesticide by In vivo detection method, observes the reaction to living body biological.The method qualitative coarse, accuracy is low, to the narrow application range of agricultural chemicals.
Instrument for detecting pesticide residue through utensil in the market has that complicated operation, volume are large, expensive, amount detection is few and the defect such as sense cycle is long, and can only use having power supply place, and need professional to operate on it, can not meet the demand of market masses, it is necessary for therefore inventing the Detecting Pesticide equipment that a kind of expense is low, simple to operate, detection speed also can be met the need of market soon.
Summary of the invention
In order to solve prior art Problems existing, the invention provides the instrument for detecting pesticide residue through that a kind of expense is low, simple to operate, accuracy is high, detection speed is fast.
The technical scheme that the present invention adopts for technical solution problem is as follows:
Instrument for detecting pesticide residue through of the present invention, comprising:
By single-chip microcomputer, the master controller that the reservoir be electrically connected with single-chip microcomputer, A/D converter and time controller form; The LED light source group be electrically connected with single-chip microcomputer respectively, microprinting device, liquid crystal touch control display screen and accumulator; The USB interface be electrically connected with reservoir; The photoelectric sensor be electrically connected with A/D converter; The power interface be connected with storage battery; The pick-up unit on same level line is all in LED light source group and photoelectric sensor;
Described LED light source group is made up of multiple LED light source, and centre wavelength is 412nm; Described pick-up unit is made up of multiple sense channel; Described LED light source and sense channel one_to_one corresponding;
By sample extracting solution, acetylcholinesterase, obtain after developer and acetylcholine mix detecting solution, be placed in each sense channel by being loaded with the cuvette detecting solution, electric power starting detector is connected by power interface, LED light source group open command is sent to single-chip microcomputer by liquid crystal touch control display screen, single-chip microcomputer opens LED light source after receiving LED light source group open command, simultaneously controller record detection time opening time, the light beam of LED light source group passes through pick-up unit, parallel radiation is on photoelectric sensor again, light beam now is absorbed a part by the detection solution in cuvette, produce absorbance, photoelectric sensor is resent in A/D converter after the light signal with absorbance is converted to electric signal, A/D converter transfers in single-chip microcomputer after the electric signal received is converted to digital signal, single-chip microcomputer carries out data processing to digital signal, calculate inhibiting rate, testing result is sent in reservoir simultaneously and stores, directly testing result can be printed by microprinting device, the detection time of time controller record and testing result are presented on liquid crystal touch control display screen all in digital form.
Described sample extracting solution is prepared by the following method: carry out pre-service to sample and obtain sample liquid, then take sample liquid 2g, add phosphate buffer 1 0ml, leaves standstill 10min or sonic oscillation 3min and obtains sample extracting solution.
Described phosphate buffer is the buffer solution of sodium hydrogen phosphate and potassium dihydrogen phosphate, purchased from traditional Chinese medicines group chemical reagent Shenyang company limited.
The addition of described sample extracting solution, acetylcholinesterase, developer and acetylcholine is respectively: sample extracting solution 1mL, acetylcholinesterase 50uL, developer 50uL and acetylcholine 50uL, obtains detecting solution after mixing.
Described developer is two thiobis paranitrobenzoic acids, produces purchased from long spring YueTong Science and Technology Ltd..
The number of described LED light source and sense channel is 20.
Temperature during detection controls at 0 DEG C ~ 40 DEG C.
Described photoelectric sensor adopts SPS0606 chip.
Described A/D converter adopts ADS1286 chip.
Described single-chip microcomputer adopts C8051F020 (22) chip.
Described time controller adopts PCF8563 (SO8) chip.
Described reservoir adopts IS62LV2568L chip.
The SG240128-01G series of display screens that described liquid crystal touch control display screen adopts Beijing collection electronic equipment manufacturing company limited to produce.
The invention has the beneficial effects as follows:
1, the present invention adopts LED light source as light source, and the spectral band scope of this light source group is narrower, reduces the requirement of monochromatic light path system, has simplified structure and the volume of detector.
2, the present invention uses liquid crystal touch control display screen keyboard and display to be combined together, alleviates detector own wt, have easy to carry, simple to operate, angle of visibility is large, transmissivity is high, low in energy consumption and the advantages such as the use of layman can be realized.
3, inside of the present invention is provided with accumulator, and not spendable shortcoming when avoiding non-transformer, furthermore achieved that whenever and wherever possible to the exploitativeness that sample detects.
4, the USB interface in the present invention, can realize the data transmission between computing machine, establishes the printing of microprinting device to be connected printer with external USB interface and print two kinds of modes in the present invention simultaneously also can realize.
5, the instruction that the present invention is sent by liquid crystal touch control display screen operates, and decreases error, effectively avoids the problem of misoperation.
6, the time controller in the present invention can provide the time, conveniently arranges and records detection time.
7, instrument for detecting pesticide residue through of the present invention is used for detecting the residual of the Organophosphorus and carbamate pesticides class agricultural chemicals in sample, can be widely used in the field quick detection of the units such as industrial and commercial bureau, detection of agricultural products center, health disease control, growing vegetables base, the market of farm produce, large supermarket, hotel, school, dining room to fruits and vegetables and Pesticide Residues In Grain.
Accompanying drawing explanation
Fig. 1 is the general structure schematic diagram of instrument for detecting pesticide residue through of the present invention.
Fig. 2 is the concrete structure schematic diagram of instrument for detecting pesticide residue through of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail.
As shown in Figure 1, instrument for detecting pesticide residue through of the present invention is made up of master controller, LED light source group, pick-up unit, accumulator, power interface, microprinting device, liquid crystal touch control display screen and USB interface.Master controller is core component of the present invention, be electrically connected with LED light source group, accumulator, microprinting device, liquid crystal touch control display screen and USB interface respectively, accumulator is electrically connected with power interface, LED light source group is made up of multiple LED light source, centre wavelength is 412nm, the number of LED light source is preferably 20, pick-up unit is made up of multiple sense channel, multiple sample can be detected simultaneously, the corresponding sense channel of each LED light source and the number of sense channel equal with the number of LED light source, the number of sense channel is preferably also 20.Be equipped with lid above sense channel, avoid the error in detection period.
As shown in Figure 2, master controller is made up of single-chip microcomputer, reservoir, photoelectric sensor, A/D converter and time controller, LED light source group, A/D converter, microprinting device, reservoir, time controller, liquid crystal touch control display screen and accumulator are electrically connected with single-chip microcomputer respectively, photoelectric sensor is electrically connected with A/D converter, USB interface is electrically connected with reservoir, and LED light source group, pick-up unit and photoelectric sensor are on same level line.
Photoelectric sensor in the present invention adopts SPS0606 chip.
A/D converter in the present invention adopts ADS1286 chip.
Single-chip microcomputer in the present invention adopts C8051F020 (22) chip.
Time controller in the present invention adopts PCF8563 (SO8) chip.
Reservoir in the present invention adopts IS62LV2568L chip.
The SG240128-01G series of display screens that liquid crystal touch control display screen in the present invention adopts Beijing collection electronic equipment manufacturing company limited to produce.
The testing process of instrument for detecting pesticide residue through of the present invention is as follows:
Before detecting sample, pre-service (according to conventional method: the green plants class samples such as grain agricultural product and leaf all need by soaking the sample liquid obtaining containing or do not contain agricultural chemicals) is carried out to sample.Take sample liquid 2g, add and extract reagent 10ml, leave standstill 10min or sonic oscillation 3min, obtain sample extracting solution, sample thief extract 1mL, acetylcholinesterase 50uL, developer 50uL and acetylcholine 50uL is added successively in cuvette, obtain after mixing detecting solution, then will put into each sense channel of pick-up unit containing the cuvette detecting solution, temperature during detection controls at 0 DEG C ~ 40 DEG C.
Said extracted reagent is phosphate buffer, is specially the buffer solution of sodium hydrogen phosphate and potassium dihydrogen phosphate, specification: AR (Shanghai examination), purchased from traditional Chinese medicines group chemical reagent Shenyang company limited; Developer is two thiobis paranitrobenzoic acids, produces purchased from long spring YueTong Science and Technology Ltd..
As shown in Figure 2, electric power starting instrument for detecting pesticide residue through is connected by power interface, LED light source group open command is sent to single-chip microcomputer by liquid crystal touch control display screen, single-chip microcomputer opens 20 LED light sources after receiving LED light source group open command, simultaneously controller writing time opening time, the light beam of LED light source group passes through sense channel, parallel radiation is on photoelectric sensor again, light beam now is absorbed a part by the detection solution in cuvette, produce absorbance, after light signal with absorbance is converted to electric signal by photoelectric sensor, be resent in A/D converter, after the electric signal received is converted to digital signal by A/D converter, finally transfer in single-chip microcomputer, single-chip microcomputer can carry out data processing to digital signal voluntarily, calculate inhibiting rate, data result after process is sent in reservoir by single-chip microcomputer, stored by reservoir, directly testing result can be printed by microprinting device, single-chip microcomputer is connected with microprinting device, can realize detecting the synchronism with print data, time controller is connected with single-chip microcomputer, conveniently arranges and records detection time, being together presented in digital form on liquid crystal touch control display screen with the testing result of residues of pesticides.
Inventive principle of the present invention:
The detection method that instrument for detecting pesticide residue through of the present invention adopts is inhibiting AChE, the similar of the substrate (acetylcholine) of Organophosphorus and carbamate pesticides class agricultural chemicals and acetylcholinesterase, under certain condition, Organophosphorus and carbamate pesticides class agricultural chemicals has inhibiting effect to acetylcholinesterase normal function, the concentration proportional of its inhibiting rate and agricultural chemicals.Under normal circumstances, enzymatic nerve conduction metabolic product (acetylcholine) is hydrolyzed, and its hydrolysate and developer react, and produces yellow substance.If not containing corresponding residues of pesticides in sample extracting solution, then the activity of the enzyme acetylcholine added is not suppressed, and the substrate component in sample extracting solution is decomposed, and its hydrolysate and developer react and produce yellow substance, have absorption maximum at 412nm place.If containing corresponding residues of pesticides in sample extracting solution, then the activity inhibited of the enzyme acetylcholine added, it is few that the substrate component in sample extracting solution is not hydrolyzed or is hydrolyzed, and its hydrolysate and developer do not react, and absorbs very weak at 412nm place.Therefore measure absorbance by spectrophotometer at 412nm place to be worth over time, calculate inhibiting rate, the residual condition of the Organophosphorus and carbamate pesticides class agricultural chemicals in sample can be judged by inhibiting rate.
During with spectrophotometer test (412nm wavelength), be calculated as follows inhibiting rate:
Inhibiting rate (%)=[(Δ Α 0-Δ Α t)/Δ Α 0] × 100%
In formula, Δ Α 0for the absorbance changing value of contrast solution, Δ Α tfor the absorbance changing value of sample solution.If sample inhibiting rate >=50%, represent that sample persticide residue exceeds standard.When detecting with instrument for detecting pesticide residue through, its inhibiting rate directly can calculate result by the data processing of single-chip microcomputer, is finally presented on liquid crystal touch control display screen.

Claims (10)

1. instrument for detecting pesticide residue through, is characterized in that, comprising:
By single-chip microcomputer, the master controller that the reservoir be electrically connected with single-chip microcomputer, A/D converter and time controller form; The LED light source group be electrically connected with single-chip microcomputer respectively, microprinting device, liquid crystal touch control display screen and accumulator; The USB interface be electrically connected with reservoir; The photoelectric sensor be electrically connected with A/D converter; The power interface be connected with storage battery; The pick-up unit on same level line is all in LED light source group and photoelectric sensor;
Described LED light source group is made up of multiple LED light source, and centre wavelength is 412nm; Described pick-up unit is made up of multiple sense channel; Described LED light source and sense channel one_to_one corresponding;
By sample extracting solution, acetylcholinesterase, obtain after developer and acetylcholine mix detecting solution, be placed in each sense channel by being loaded with the cuvette detecting solution, electric power starting detector is connected by power interface, LED light source group open command is sent to single-chip microcomputer by liquid crystal touch control display screen, single-chip microcomputer opens LED light source after receiving LED light source group open command, simultaneously controller record detection time opening time, the light beam of LED light source group passes through pick-up unit, parallel radiation is on photoelectric sensor again, light beam now is absorbed a part by the detection solution in cuvette, produce absorbance, photoelectric sensor is resent in A/D converter after the light signal with absorbance is converted to electric signal, A/D converter transfers in single-chip microcomputer after the electric signal received is converted to digital signal, single-chip microcomputer carries out data processing to digital signal, calculate inhibiting rate, testing result is sent in reservoir simultaneously and stores, directly testing result can be printed by microprinting device, the detection time of time controller record and testing result are presented on liquid crystal touch control display screen all in digital form.
2. instrument for detecting pesticide residue through according to claim 1, it is characterized in that, described sample extracting solution is prepared by the following method: carry out pre-service to sample and obtain sample liquid, take sample liquid 2g again, add phosphate buffer 1 0ml, leave standstill 10min or sonic oscillation 3min and obtain sample extracting solution.
3. instrument for detecting pesticide residue through according to claim 2, is characterized in that, described phosphate buffer is the buffer solution of sodium hydrogen phosphate and potassium dihydrogen phosphate, purchased from traditional Chinese medicines group chemical reagent Shenyang company limited.
4. instrument for detecting pesticide residue through according to claim 1, it is characterized in that, the addition of described sample extracting solution, acetylcholinesterase, developer and acetylcholine is respectively: sample extracting solution 1mL, acetylcholinesterase 50uL, developer 50uL and acetylcholine 50uL, obtains detecting solution after mixing.
5. instrument for detecting pesticide residue through according to claim 1, is characterized in that, described developer is two thiobis paranitrobenzoic acids, produces purchased from long spring YueTong Science and Technology Ltd..
6. instrument for detecting pesticide residue through according to claim 1, is characterized in that, the number of described LED light source and sense channel is 20.
7. instrument for detecting pesticide residue through according to claim 1, is characterized in that, temperature during detection controls at 0 DEG C ~ 40 DEG C.
8. instrument for detecting pesticide residue through according to claim 1, is characterized in that, described photoelectric sensor adopts SPS0606 chip; Described A/D converter adopts ADS1286 chip.
9. instrument for detecting pesticide residue through according to claim 1, is characterized in that, described single-chip microcomputer adopts C8051F020 (22) chip; Described time controller adopts PCF8563 (SO8) chip.
10. instrument for detecting pesticide residue through according to claim 1, is characterized in that, described reservoir adopts IS62LV2568L chip; The SG240128-01G series of display screens that described liquid crystal touch control display screen adopts Beijing collection electronic equipment manufacturing company limited to produce.
CN201410675349.5A 2014-11-21 2014-11-21 Pesticide residue detector Pending CN104374723A (en)

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Cited By (8)

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Publication number Priority date Publication date Assignee Title
CN106248893A (en) * 2016-09-21 2016-12-21 成都创慧科达科技有限公司 A kind of detection device based on multiple sensors
CN106501424A (en) * 2016-11-10 2017-03-15 嘉兴职业技术学院 The residual detection method of agriculture based on automatization's preprocessing system
CN106770699A (en) * 2016-11-10 2017-05-31 嘉兴职业技术学院 A kind of fruit and vegetable residual pesticide detecting system
CN106770258A (en) * 2017-01-17 2017-05-31 深圳市安鑫宝科技发展有限公司 Intelligent digital type rapid detection for pesticide residue apparatus and method
CN107677778A (en) * 2017-11-08 2018-02-09 苏州市苏测检测技术有限公司 Hand-hold food device for fast detecting
CN108398394A (en) * 2018-04-24 2018-08-14 武汉中科志康生物科技有限公司 Full-automatic instrument for detecting pesticide residue through
CN110736702A (en) * 2019-11-20 2020-01-31 丁春旭 multifunctional pesticide residue detection control system
CN111308008A (en) * 2020-04-02 2020-06-19 重庆瑞钛科技有限公司 Method for rapidly detecting starch content in grain for wine brewing

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CN204255847U (en) * 2014-11-21 2015-04-08 长春长光思博光谱技术有限公司 Instrument for detecting pesticide residue through

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CN204255847U (en) * 2014-11-21 2015-04-08 长春长光思博光谱技术有限公司 Instrument for detecting pesticide residue through

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106248893A (en) * 2016-09-21 2016-12-21 成都创慧科达科技有限公司 A kind of detection device based on multiple sensors
CN106501424A (en) * 2016-11-10 2017-03-15 嘉兴职业技术学院 The residual detection method of agriculture based on automatization's preprocessing system
CN106770699A (en) * 2016-11-10 2017-05-31 嘉兴职业技术学院 A kind of fruit and vegetable residual pesticide detecting system
CN106770258A (en) * 2017-01-17 2017-05-31 深圳市安鑫宝科技发展有限公司 Intelligent digital type rapid detection for pesticide residue apparatus and method
CN106770258B (en) * 2017-01-17 2019-03-19 深圳市安鑫宝科技发展有限公司 Intelligent digital type rapid detection for pesticide residue device and method
CN107677778A (en) * 2017-11-08 2018-02-09 苏州市苏测检测技术有限公司 Hand-hold food device for fast detecting
CN108398394A (en) * 2018-04-24 2018-08-14 武汉中科志康生物科技有限公司 Full-automatic instrument for detecting pesticide residue through
CN110736702A (en) * 2019-11-20 2020-01-31 丁春旭 multifunctional pesticide residue detection control system
CN111308008A (en) * 2020-04-02 2020-06-19 重庆瑞钛科技有限公司 Method for rapidly detecting starch content in grain for wine brewing

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