CN103558272B - A kind of rapid detector for antibiotic residue of aptamer sensor - Google Patents
A kind of rapid detector for antibiotic residue of aptamer sensor Download PDFInfo
- Publication number
- CN103558272B CN103558272B CN201310576568.3A CN201310576568A CN103558272B CN 103558272 B CN103558272 B CN 103558272B CN 201310576568 A CN201310576568 A CN 201310576568A CN 103558272 B CN103558272 B CN 103558272B
- Authority
- CN
- China
- Prior art keywords
- aptamer sensor
- electrode
- circuit
- antibiotic residue
- detector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of rapid detector for antibiotic residue of aptamer sensor, by aptamer sensor, input and disposal system, display and printing storage system, electric power system forms.With the working electrode that shitosan, glutaraldehyde and potassium ferricyanide compound are modified, then with nano Au colloid, aptamers is fixed to electrode surface, preparation aptamer sensor.The change detecting electric current before and after aptamer sensor contact microbiotic measures antibiotic concentration.The low current signal of collection is carried out I/V conversion by signal processing circuit, amplifies, filtering, and routine processes is carried out in A/D conversion the most at last digital quantity signal feeding microcontroller; Whether this detector can exceed standard to the antibiotic residual quantity detected in sample and concentration value shows, stores and prints.Whether rapid detector for antibiotic residue of aptamer sensor of the present invention can detect containing information such as certain microbiotic in sample quickly and accurately, is applicable to very much the occasion of antibiotic residue being carried out to field quick detection.
Description
Technical field
The present invention relates to a kind of rapid detector for antibiotic residue, particularly relate to a kind of rapid detector for antibiotic residue of aptamer sensor, belong to agricultural product security detection technique field.
Background technology
In recent years, along with developing rapidly of China's livestock breeding industry, milk yield increases substantially, and fresh milk and dairy produce have become the important component part in the people (especially old man and children) life food.But the widespread use of microbiotic in Modern Animal Husbandry, inevitably causes Residue of Antibiotics in Milk.If drink containing antibiotic milk for a long time, equal the microbiotic of long-term taking low dose beyond doubt, after the people of microbiotic allergic constitution being taken to the milk of residual antibiotic, can allergic reaction be caused.Normal drinking person, can cause some the conditionality pathogenic bacteria in body easily to produce drug resistance, be difficult to prove effective again once ill with antibiotic therapy of the same race.Antibiotic residue not only harm humans is healthy, also affects the quality of milk simultaneously, causes economic loss.Because the mastadenitis of cow incidence of disease is higher, usually treat the obstetric conditions such as mastitis still comparatively general with antibiosis, this easily causes the residual of in milk tetracycline.Excessive use microbiotic inevitably makes the associated antibiotics such as parent metabolic product residue in the muscle of animal, egg, milk, organs and tissues, and then affects health by food chain.Visible, strengthen the detection to antibiotic residue in the agricultural product such as milk, especially ensure that human health has very profound significance.
Traditional method for antibiotic residue detection mainly contains: gas chromatography (GC), high performance liquid chromatography (HPLC), Chromatography/Mass Spectrometry coupling technique (GC/LC-MS), capillary electrophoresis (CE), fluorescence analysis, euzymelinked immunosorbent assay (ELISA) (ELISA).Although these method selectivity are good, highly sensitive, accuracy is high, detection limit is low, can detect multiple element or compound, but it needs expensive instrument and equipment, sample pretreatment process is loaded down with trivial details, time-consuming simultaneously, and require very high to the technical merit of analyst, be unsuitable for field quick detection.Compared with traditional analytical approach, biology sensor has following features: the selectivity that (1) is higher, does not therefore need to be separated tested component, namely need not carry out pre-service to sample.(2) structure is simple, and volume is little, easy to use, particularly portable immunosensor, is very beneficial for the Fast Measurement of agricultural product security quality.(3) can continuous on-line detection be realized, make the quality control of food processing process become easy.(4) fast response time, amount of samples is few, and compared with other large-sized analytic instruments, immunosensor cost of manufacture is low, and can Reusability.
The object of invention is for the deficiencies in the prior art, and design provides a kind of aptamer sensor antibiotics leftover detection instrument, have easy to carry, detect quick and precisely, the feature such as simple to operate, the online qualitative, quantitative measurement that can realize antibiotic residue.
The ultimate principle of a kind of rapid detector for antibiotic residue of aptamer sensor of the present invention is: aptamer sensor is contacted before and after microbiotic and causes due to immune response the change of low current signal to be gathered signal by testing circuit by three-electrode system.The low current signal collected is carried out I/V conversion by testing circuit, amplifies, filtering, and routine processes is carried out in A/D conversion the most at last digital quantity signal feeding single-chip microcomputer.Finally, data by parameter displays such as the concentration of antibiotic residue, degree of suppression out, and store so that user checks in time and carries out data analysis by display and printing storage system.Detector of the present invention includes mini-printer, data can be carried out corresponding printout.The single-chip microcomputer of input and disposal system inside carries out programming Control to showing with printing storage system is unified.
For realizing above function, the preparation method of the aptamer sensor of a kind of rapid detector for antibiotic residue of aptamer sensor of the present invention is: the preparation of (1) shitosan, glutaraldehyde and potassium ferricyanide compound: first configure the acetum of 1% (1 ml/100 ml water) and prepare the chitosan solution of 50 ml 1% (1 g/100 ml) with the acetum prepared as solvent.The K3 of 5 mM of 500 μ l [Fe (CN) 6] is mixed ultrasonic vibration with the chitosan solution (400 μ l 1% shitosan+600 μ l water) of 1 mL and within 5 minutes, obtains limpid yellow solution.The glutaraldehyde adding 100 μ l 5% in appeal solution left standstill 24h after ultrasonic 10 minutes, prepared by shitosan, glutaraldehyde and potassium ferricyanide compound.(2) method preparing employing trisodium citrate thermal reduction gold chloride (HAuCl4) of nano Au colloid.All glasswares all soak with chloroazotic acid, and washes clean is for subsequent use.Configure the chlorauric acid solution of 100 mL 0.01%, be heated to boiling with vigorous stirring, then 2.5 mL 1% citric acid three sodium solutions are added rapidly, continue stir 20min (around here solution by faint yellow transfer to colourless, then grey black is become, finally become claret), slowly cool under continuing to be stirred to room temperature after removing thermal source.Cooled gold size solution is placed in clean Brown Glass Brown glass bottles and jars only to keep in Dark Place for subsequent use in 4 ° of C refrigerators, and the several months is effective.(3) compound getting shitosan, glutaraldehyde and the potassium ferricyanide that 5 μ L prepare drips and is coated in the good glassy carbon electrode surface of pre-service, air drying, with the silver orthophosphate wash buffer electrode surface of pH7.5.(4) after electrode surface dries, get 5 μ L nano Au colloid and drip and be coated in electrode surface, leave standstill to drying under normal temperature, then by the nm of gold of ultrapure water surface physics absorption, nitrogen dries up.(8) then the aptamers of 5 μ L 5mM is dropped in and above-mentionedly on nanometer-material-modified good electrode, cover the evaporation preventing moisture with rubber cap, with ultrapure water surface after 4h, dry stand-by.(9) finally the above-mentioned electrode prepared is dipped in and closes 1h with the mercaptoethanol of 1M, and with closed nonspecific binding site, aptamer sensor completes, for subsequent use under being kept at 4 ° of C conditions.
Described a kind of rapid detector for antibiotic residue of aptamer sensor, is characterized in that: three-electrode system circuit by three electrode aptamer sensor, three electrode detection circuit, potentiostatic circuit form; Three electrode immunosensors gather current signal and are transmitted to input and disposal system circuit by three electrode detection circuit.Potentiostatic circuit provides the burning voltage of 500mV to reaction solution environment.
Input and disposal system circuit have I/V to be changed, and amplifies, filter function, includes A/D conversion chip and the analog signals of enzyme sensor collection after I/V conversion is converted to digital quantity signal and send into single-chip microcomputer and carry out routine processes.The singlechip microcontroller of input and disposal system is STC89C52.The trace routine of single-chip microcomputer comprises data-printing and storage, time showing, operation instruction and the dialog box to important step prompting, helps user grasp the using method of instrument fast and carry out inspection and the process of data after carrying out data-printing and convenient storage.Input and disposal system circuit adopt TLC272CP chip multipath discharge circuit to have I/V and change, and amplify, and filtering also provides the function of 500mv constant voltage to three electrode reaction environment.
Display and printing storage system circuit are by display circuit, and print circuit and memory circuitry form.Display is undertaken controlling carrying out as user the interface that controls by operation push-button for showing data.Printing storage system circuit is connected with mini-printer and storer respectively and carries out data-printing and storage.Display screen have employed the general display screen of 3.5 cun, and mini-printer adopts micro embedded printer, and its size and power consumption meet and be convenient for carrying and portable power consumption requirements.
For reaching above object, following technical scheme is taked to realize: electric power system electric routing switch power supply and voltage conversion circuit composition, alternating current is converted to direct current and powers respectively to each circuit system and mini-printer through voltage conversion circuit by Switching Power Supply.Switching Power Supply is+12V, 1A ,-12V, 1A ,+5V, and 5A tri-tunnel exports.Power supply is connected with the Switching Power Supply in electric power system.
Accompanying drawing explanation
Fig. 1 is the structured flowchart of a kind of rapid detector for antibiotic residue of aptamer sensor of the present invention,
Fig. 2 is that the present invention three electrode and potentiostatic circuit and A/D change treatment circuit in early stage.
Embodiment
First, structural schematic block diagram of the present invention is illustrated; Secondly, the potentiostatic circuit included by input and disposal system, A/D conversion treatment circuit in early stage are described in detail; Finally, the application software of whole system and testing process are illustrated.
Fig. 1 is the structured flowchart of a kind of rapid detector for antibiotic residue of aptamer sensor of the present invention.As shown in Figure 1, the present invention is by three-electrode system, and input and disposal system, display and printing storage system, electric power system forms.Three-electrode system gathers the low current signal in reaction solution by immunosensor; The low current signal that three electrodes collect by input and disposal system carries out I/V conversion, amplifies, filtering, and routine processes is carried out in A/D conversion the most at last digital quantity signal feeding single-chip microcomputer; Data by the residual parameter display such as antibiotic concentration, degree of suppression out, and store so that user checks in time and carries out data analysis by display and printing storage system.Detector of the present invention includes mini-printer, data can be carried out corresponding printout.The single-chip microcomputer of input and disposal system inside carries out programming Control to showing with printing storage system is unified.It is that each circuit system and mini-printer are powered that alternating current is converted to three road direct currents by electric power system.Described microcontroller employing kernel is the STC89C52RC+ microcontroller of MCS-51, and it loads trace routine.
In the present invention, the potentiostatic circuit in testing circuit provides stable operating voltage for three-electrode system current/voltage transforms testing circuit, and Fig. 2 is an embodiment of potentiostat circuit, by two-way operational amplifier U10 TLC272CP and R44, R46, R4, C35, C16, R48 are formed.The pin 6 of amplifier B is connected with 7 and is connected with 3 pin of A, 2 pin of A are connected with the working electrode of three-electrode system and have connected C16, strengthen antijamming capability, R48 be as feedback resistance on amplifier A, the external potentiometer R46 of 5 pins of B, by positive 5V for electric excitation, R44, R46, R4 is formed potentiostatic circuit by voltage supply 500mv voltage with 5 pins of B, initial voltage be 500mv. amplifier A and pin 8 connect+5V power supply, pin 4 meets-5V, the amplifier B pin 7 of U9 TLC272CP is directly connected as voltage follower with pin 6, ADE pin 3 is connected with the pin 5 of B, common ground, play jamproof effect.The pin 2 of A is directly connected with the contrast electrode of three-electrode system, pin 1 is directly connected with the auxiliary electrode of three-electrode system, between connect electric capacity 14, object has been the effect of buffer circuit, load-carrying ability can be improved simultaneously, therefore U9 and U10 together constitutes the three-electrode system of biology sensor, and test side used, by drawing, gathers the low current signal produced with detection electrochemical reaction here.
The aptamer sensor preparation method of a kind of rapid detector for antibiotic residue of aptamer sensor of the present invention is: the preparation of (1) shitosan, glutaraldehyde and potassium ferricyanide compound: first configure the acetum of 1% (1 ml/100 ml water) and prepare the chitosan solution of 50 ml 1% (1 g/100 ml) with the acetum prepared as solvent.The K3 of 5 mM of 500 μ l [Fe (CN) 6] is mixed ultrasonic vibration with the chitosan solution (400 μ l 1% shitosan+600 μ l water) of 1 mL and within 5 minutes, obtains limpid yellow solution.The glutaraldehyde adding 100 μ l 5% in appeal solution left standstill 24h after ultrasonic 10 minutes, prepared by shitosan, glutaraldehyde and potassium ferricyanide compound.(2) method preparing employing trisodium citrate thermal reduction gold chloride (HAuCl4) of nano Au colloid.All glasswares all soak with chloroazotic acid, and washes clean is for subsequent use.Configure the chlorauric acid solution of 100 mL 0.01%, be heated to boiling with vigorous stirring, then 2.5 mL 1% citric acid three sodium solutions are added rapidly, continue stir 20min (around here solution by faint yellow transfer to colourless, then grey black is become, finally become claret), slowly cool under continuing to be stirred to room temperature after removing thermal source.Cooled gold size solution is placed in clean Brown Glass Brown glass bottles and jars only to keep in Dark Place for subsequent use in 4 ° of C refrigerators, and the several months is effective.(3) compound getting shitosan, glutaraldehyde and the potassium ferricyanide that 5 μ L prepare drips and is coated in the good glassy carbon electrode surface of pre-service, air drying, with the silver orthophosphate wash buffer electrode surface of pH7.5.(4) after electrode surface dries, get 5 μ L nano Au colloid and drip and be coated in electrode surface, leave standstill to drying under normal temperature, then by the nm of gold of ultrapure water surface physics absorption, nitrogen dries up.(8) then the aptamers of 5 μ L 5mM is dropped in and above-mentionedly on nanometer-material-modified good electrode, cover the evaporation preventing moisture with rubber cap, with ultrapure water surface after 4h, dry stand-by.(9) finally the above-mentioned electrode prepared is dipped in and closes 1h with the mercaptoethanol of 1M, and with closed nonspecific binding site, aptamer sensor completes, for subsequent use under being kept at 4 ° of C conditions.
Testing process of the present invention is: first, the aptamer sensor prepared is put into not containing liquid at the bottom of antibiotic test, records not containing electric current I during microbiotic
1, electric current (default value that before suppressing, electric current also can set in employing system) before namely suppressing; Afterwards aptamer sensor is removed, after distilled water flushing, aptamer sensor working electrode is put into detected sample liquid and hatches 10 minutes, again aptamer sensor is put into test end liquid, record the electric current I after suppression
2; Automatically calculate inhibiting rate (I%) finally by the trace routine that microcontroller runs, computing formula is as follows:
I%
=(I
1- I
2)/ I
1 ×100%
If usually detected containing microbiotic in sample liquid, hinder electro transfer between test fluid and electrode, electric current I because aptamers and microbiotic generation immune response produce immune complex
2be less than I
1.I
1with I
2the larger then inhibiting rate of difference larger, show that the concentration detecting antibiotic residue in sample liquid is higher; I
1with I
2the less then inhibiting rate of difference less, show to detect the concentration of antibiotic residue in sample liquid lower.
Think at present when inhibiting rate be greater than, equal 50% time, antibiotic residue exceeds standard; When inhibiting rate is less than 50%, antibiotic residue does not exceed standard.
Microcontroller makes qualitative conclusions according to the inhibiting rate obtained, and result is delivered to display screen display, and by end product printout, data carry out storing for later the searching and studying of related personnel simultaneously in the lump.
The trace routine that microcontroller of the present invention runs also comprises screen redirect demonstration and dialog box, print prompt to important step prompting, and user can be helped to grasp the using method of instrument fast.
The embodiment of trace routine: first, puts into substrate solution and carries out detection preparation by immunosensor.Then, press instrument switch key, enter homepage after system initialization, display screen side has test button, reset and printing three to broadcast mill button.First time clicks test button, starts to detect and shows reading I
1; Second time observes prompting after clicking and detecting, display result I
2; Click and print, print result is also preserved.Opening power key, screen display " START detection ", resets.Crocodile clip is stepped up respectively three columnar electrodes that three-electrode system is corresponding, Pen-down detection key in the phosphate buffered solution prepared before the immunosensor of sessile antibody is immersed in, screen display starts to detect, after about 1min, baseline reaches maintenance level, after about 30s, obtain first complete current data, be designated as I
1.Testing sample is tested: immunosensor is put into the sample solution extracted, about 10min, immersed in quantification of phosphate buffer solution afterwards again, press measurement key, clicks and detects the measurement of key second time, show second current data I simultaneously
2.Automatically the maximum inhibition of this sample liquid to immobilised enzymes is calculated by two current data.Select suitable relation curve to calculate the target antibiotic concentration of extract, and be converted to the antibiotic content of target in sample, the report of the last measurement result of generation automatically.Press printing key, the data being printed on front and back twice detection voltage, microbiotic inhibiting rate and microbiotic detectable concentration are carried out printout.
One of the present invention is rapid detector for antibiotic residue of aptamer sensor, operating procedure is simple, detection time is shorter, the qualitative, quantitative that can realize antibiotic residue is measured, have highly sensitive, good stability, high repeatability and other advantages, meet China's antibiotic residue Fast Detection Technique development and internationalization requirement.
Claims (4)
1. a rapid detector for antibiotic residue of aptamer sensor, is characterized in that: this detector by three electrode aptamer sensor, input and treatment circuit, display and printing memory circuit, constant potential feed circuit, operation push-button, data-carrier store, mini-printer, electric power generating composition; The current signal that immunosensor produces is transmitted to input and disposal system circuit by testing circuit; Potentiostatic circuit is to the stable operating voltage providing 500mV between working electrode and contrast electrode; Described input and treatment circuit have I/V to be changed, amplify, filter function, include A/D conversion chip the analog signals of aptamer sensor collection is converted to digital quantity signal send into single-chip microcomputer carry out routine processes; After described immunosensor uses nanometer-material-modified working electrode, specific microbiotic aptamers is fixed to electrode surface, whether can contains certain microbiotic in the measurement sample liquid of specificity;
Described aptamer sensor, prepare by the following method:
1) preparation of shitosan, glutaraldehyde and potassium ferricyanide compound: the first acetum of dose volume than 1% be the chitosan solution that 50mL mass volume ratio 1% prepared by solvent with the acetum prepared, 500 μ L5mM K3 [Fe (CN) 6] are mixed ultrasonic vibration with the chitosan solution of 1ml and within 5 minutes, obtains limpid yellow solution, the glutaraldehyde adding 100 μ L 5% in above-mentioned solution leaves standstill 24h after ultrasonic 10 minutes again, prepared by shitosan, glutaraldehyde and potassium ferricyanide compound;
The chitosan solution of described 1ml, the water of the shitosan containing 400 μ L 1% and 600 μ L;
2) method preparing employing trisodium citrate thermal reduction gold chloride of nano Au colloid, all glasswares all soak with chloroazotic acid, for subsequent use after washes clean; The chlorauric acid solution of preparation 100mL 0.01%, be heated to boiling with vigorous stirring, then the citric acid three sodium solution of 2.5mL 1% is added rapidly, continue to stir 20min, slowly cool under continuing to be stirred to room temperature after removing thermal source, cooled gold size solution is placed in clean Brown Glass Brown glass bottles and jars only to keep in Dark Place for subsequent use in 4 DEG C of refrigerators, and the several months is effective;
3) compound getting shitosan, glutaraldehyde and the potassium ferricyanide that 5 μ L prepare drips and is coated in the good glassy carbon electrode surface of pre-service, air drying, rinses electrode surface by the phosphate-buffered of pH7.5;
4) after electrode surface dries, get 5 μ L nano Au colloid and drip and be coated in electrode surface, leave standstill to drying under normal temperature, then by the nm of gold of ultrapure water surface physics absorption, nitrogen dries up;
5) aptamers of 5 μ L 5mM is dropped in above-mentioned on nanometer-material-modified good electrode, cover with rubber cap and prevent moisture from evaporating, with ultrapure water surface after 4h, dry stand-by;
6) finally the mercaptoethanol that the above-mentioned electrode prepared is dipped in 1M is closed 1h, with closed nonspecific binding site, aptamer sensor completes, for subsequent use under preserving 4 DEG C of conditions.
2. a kind of rapid detector for antibiotic residue of aptamer sensor as claimed in claim 1, is characterized in that the singlechip microcontroller of described input and disposal system is STC89C52.
3. a kind of rapid detector for antibiotic residue of aptamer sensor as claimed in claim 1, it is characterized in that this detector uses immunosensor, specific microbiotic aptamers is secured at aptamer sensor working electrode surface, therefore whether this detector can realize containing certain microbiotic in the working sample of specificity, and the antibiotic residue qualitative, quantitative realizing sample is measured.
4. a kind of rapid detector for antibiotic residue of aptamer sensor as claimed in claim 1, it is characterized in that described input and disposal system circuit adopt TLC272CP chip multipath discharge circuit, there is I/V conversion, amplify, filtering also provides the function of 500mv constant voltage to three electrode reaction environment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310576568.3A CN103558272B (en) | 2013-11-19 | 2013-11-19 | A kind of rapid detector for antibiotic residue of aptamer sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310576568.3A CN103558272B (en) | 2013-11-19 | 2013-11-19 | A kind of rapid detector for antibiotic residue of aptamer sensor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103558272A CN103558272A (en) | 2014-02-05 |
| CN103558272B true CN103558272B (en) | 2015-07-29 |
Family
ID=50012581
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310576568.3A Active CN103558272B (en) | 2013-11-19 | 2013-11-19 | A kind of rapid detector for antibiotic residue of aptamer sensor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103558272B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103940988B (en) * | 2014-04-10 | 2015-12-09 | 山东理工大学 | A kind of heavy metal fast detector based on enzyme immunosensor and detection method thereof |
| CN104535729A (en) * | 2014-12-23 | 2015-04-22 | 温岭市太平高级职业中学 | Portable antibiotic detection pocket |
| CN104931544A (en) * | 2015-06-11 | 2015-09-23 | 山东理工大学 | Method of producing microarray aptamer sensor used for antibiotic residue detection |
| CN106841335B (en) * | 2016-09-21 | 2019-04-30 | 青岛农业大学 | A kind of preparation method of antibiotic self energizing aptamer sensor |
| CN106841346A (en) * | 2016-12-22 | 2017-06-13 | 山东理工大学 | A kind of palm aflatoxin fast detector based on Internet of Things |
| CN106483175B (en) * | 2016-12-22 | 2019-06-11 | 山东理工大学 | A kind of palm rapid detector for antibiotic residue based on Internet of Things |
| CN106872539A (en) * | 2017-03-29 | 2017-06-20 | 深圳市赛亿科技开发有限公司 | The intelligent chopsticks of clenbuterol content in a kind of detection meat |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1699985A (en) * | 2004-05-18 | 2005-11-23 | 中国科学院研究生院应用化学研究所 | Process for making nano-modification minisize electrochemical biosensor for pesticide detection |
| CN101661015A (en) * | 2009-09-09 | 2010-03-03 | 上海睿兴实业有限公司 | Universal portable detector for electrochemical biosensor |
| CN102095772A (en) * | 2011-01-27 | 2011-06-15 | 上海交通大学 | Pesticide residue detecting instrument based on three-electrode enzyme sensor |
| CN102103122A (en) * | 2011-01-26 | 2011-06-22 | 山东理工大学 | Rapid detector and detection method for electrochemical pesticide residue |
| CN102914583A (en) * | 2012-05-07 | 2013-02-06 | 赵朝辉 | Portable electrochemistry biochip detector system |
| CN103335972A (en) * | 2013-07-16 | 2013-10-02 | 江南大学 | Detection method of kanamycin residue based on nucleic acid aptamer |
-
2013
- 2013-11-19 CN CN201310576568.3A patent/CN103558272B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1699985A (en) * | 2004-05-18 | 2005-11-23 | 中国科学院研究生院应用化学研究所 | Process for making nano-modification minisize electrochemical biosensor for pesticide detection |
| CN101661015A (en) * | 2009-09-09 | 2010-03-03 | 上海睿兴实业有限公司 | Universal portable detector for electrochemical biosensor |
| CN102103122A (en) * | 2011-01-26 | 2011-06-22 | 山东理工大学 | Rapid detector and detection method for electrochemical pesticide residue |
| CN102095772A (en) * | 2011-01-27 | 2011-06-15 | 上海交通大学 | Pesticide residue detecting instrument based on three-electrode enzyme sensor |
| CN102914583A (en) * | 2012-05-07 | 2013-02-06 | 赵朝辉 | Portable electrochemistry biochip detector system |
| CN103335972A (en) * | 2013-07-16 | 2013-10-02 | 江南大学 | Detection method of kanamycin residue based on nucleic acid aptamer |
Non-Patent Citations (2)
| Title |
|---|
| Electrochemical sensors for detection of tetracycline antibiotics;Faridah S等;《Malaysian Journal of Animal Science》;20121231;第15卷(第1期);第67-80页 * |
| Label-free electrochemical immunosensor based on K3[Fe(CN)6] as signal for facile and sensitive determination of tumor necrosis factor-alpha;Shaohuang Weng等;《Sensors and Actuators B: Chemical》;20130422;第184卷;第1-7页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103558272A (en) | 2014-02-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103558272B (en) | A kind of rapid detector for antibiotic residue of aptamer sensor | |
| CN101458223B (en) | Preparation of quantitative rapid detecting sensor of microcapsule algae toxin and applications | |
| CN103529111B (en) | Quick pesticide residue detector of acetylcholin esterase biosensor | |
| CN106248748B (en) | A kind of acetylcholinesterasebiosensor biosensor and its application | |
| CN103424448A (en) | Method for detecting trace ochratoxin A (OTA) by adopting electrochemical aptamer sensor | |
| CN108445057A (en) | A kind of preparation and analysis method for detecting the electrochemical sensor of heavy metal ion | |
| CN106841335B (en) | A kind of preparation method of antibiotic self energizing aptamer sensor | |
| CN103558374B (en) | Quick pesticide residue detector with current-mode immunosensor | |
| CN104374723A (en) | Pesticide residue detector | |
| US20220308005A1 (en) | Single-cell-based Electrochemical Sensor based on Functionalized Nano-probe and Application thereof | |
| CN102608187A (en) | Preparation method of hollow gold nanometer particle-modified acetylcholinesterase biosensor | |
| CN103713026A (en) | Preparation method and applications of aptamer electrochemical sensor for detecting malachite green (MG) | |
| CN103529114A (en) | Rapid domestic fruit and vegetable pesticide residue detection instrument | |
| CN102980935B (en) | Electrochemical method for detecting anthracene-phenanthrene resultant of polycyclic aromatic hydrocarbon | |
| CN101995462A (en) | Preparation and application of label-type electrochemical immunosensor for detecting veterinary drug residues | |
| Li et al. | Magnetic beads-based electrochemical immunosensor for detection of pseudorabies virus antibody in swine serum | |
| CN103630587B (en) | A kind of fast, the method for Sensitive Detection organophosphorus pesticide chlopyrifos | |
| Zhang et al. | Sensitive and selective determination of riboflavin in milk and soymilk powder by multi-walled carbon nanotubes and ionic liquid [BMPi] PF 6 modified electrode | |
| CN104914146A (en) | Antibiotic residue detector based on screen-printed electrode aptamer sensor | |
| CN204255847U (en) | Instrument for detecting pesticide residue through | |
| CN101858918B (en) | Method for detecting ractopamine in animal-derived food by microgap array electrode-based electrochemical immunosensor | |
| CN102520030B (en) | Manufacturing method of label-free electrochemical immunosensor for detecting zearalanol | |
| CN113607792A (en) | Rapid blood fat detector and detection method | |
| Ma et al. | A novel sensitive electrochemical method for the detection of ractopamine in meat food via polycitrulline-modified electrode | |
| CN111239212B (en) | Ciprofloxacin detection method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |