CN106222081A - Single centre multi-channel assembled detection device - Google Patents
Single centre multi-channel assembled detection device Download PDFInfo
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- CN106222081A CN106222081A CN201610798408.7A CN201610798408A CN106222081A CN 106222081 A CN106222081 A CN 106222081A CN 201610798408 A CN201610798408 A CN 201610798408A CN 106222081 A CN106222081 A CN 106222081A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
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Abstract
The present invention relates to a kind of single centre multi-channel assembled detection device, including detection plate, described detection plate is provided with the detection zone of at least one, and described detection zone includes being loaded plummer and being located at multiple reactive tanks of described sample-adding plummer periphery；Described sample-adding plummer center or side are provided with the first well, are extended with multiple drainage channel for starting point to sample-adding plummer surrounding edge or opposite side week edge with described first well, and described drainage channel is connected with reactive tank one_to_one corresponding；Inner bottom surface along described drainage channel is provided with some grades of steps.This device is microflow control technique, makes each sample pipetting volume equal；And realization carries out one-time detection to a sample and determines the detection pattern of plurality of target detectable substance.
Description
Technical field
The present invention relates to biochemical analysis apparatus field, be specifically related to a kind of single centre multi-channel assembled detection device.
Background technology
The most most detectable is all single index detection at present, but, along with biomedical detection technique
Development, being constantly exploited of various emerging, valuable Testing index, the testing result data of single index are single, no
Enough situations comprehensively reflecting disease, the detection of many index is particularly important.
For above-mentioned situation, single index monitoring device is gradually improved by this field, such as patent application document
A kind of novel multinomial joint inspection reagent paper disclosed in 200520128615.9, in its multinomial structure, each first monitoring shares a sample application zone,
Only need to once be loaded when sample-adding, operate the easiest, but the design of the sample application zone of this structure is excessively simple, fails to realize sample
Divide equally, occur sample distribution between every detectable uneven due to the difference of test strips water absorbing properties in actual use
Situation, ultimately results in the deviation of testing result.And for example a kind of multi objective dish disclosed in patent application document 201520435678.2
Formula dot immuno gold filtration assay detection device, including base plate, cover plate, water suction packing ring and carrier film, described base plate center is provided with center
Sample application zone, sample application zone, described center is provided around more than one fan-shaped reaction zone, and the most described base plate is additionally provided with annular slot；Institute
State annular water suction packing ring to be placed in described annular slot；Described carrier film is placed in sample application zone, described center and described fan-shaped reaction zone
In, adjacent with described annular water suction packing ring；Described cover plate pushes down described carrier film and described water suction packing ring, and described cover plate covers in institute
State on base plate；This device once can detect multiple index, simple to operation fast, there is multi-channel parallelization operation excellent
Gesture.But center sample-adding amount and each sample pipetting volume amount cannot be controlled by this device simultaneously.
Summary of the invention
It is an object of the invention to overcome defect of the prior art, a kind of single centre of design multi-channel assembled detection dress
Put, for microflow control technique, make each sample pipetting volume equal；Realize that a sample is carried out one-time detection and determine that plurality of target detects
The detection pattern of thing, solves fluid sample distribution inequality present in joint-detection, and fluid sample circulation is too fast causes overflow to damage
The problems such as mistake so that multinomial detection can be carried out by Simultaneous Stabilization.
For achieving the above object, the technical solution adopted in the present invention is a kind of single centre multi-channel assembled detection dress
Putting, including detection plate, described detection plate is provided with the detection zone of at least one, and described detection zone includes being loaded plummer and being located at institute
State multiple reactive tanks of sample-adding plummer periphery；Described sample-adding plummer center or side are provided with the first well, with institute
Stating the first well is that starting point is extended with multiple drainage channel to sample-adding plummer surrounding edge or opposite side week edge, and described drain is led to
Road is connected with reactive tank one_to_one corresponding；Inner bottom surface along described drainage channel is provided with some grades of steps.
Further in preferred version, described some grades of steps are along decline step by step or first to go up to periphery from the first well
The step declined after Shenging.
Further preferred version is, described sample-adding plummer is additionally provided with the second well, described second well with
Described drainage channel is vertically connected logical, and described second well is located at the outer end of high-order step, can avoid the second well
The liquid added flows backward to the phenomenon of the first well.
Further, described reactive tank is carried out around arrangement or centrosymmetry arrangement centered by sample-adding plummer；Or
Centered by sample-adding plummer, axle carries out axial symmetry arrangement.
Further, described some grades of steps are for being provided with some level to sample-adding plummer week along end from the first well position
Step.
Further, the drainage channel section between described first well and the second well is provided with and laterally or vertically inserts
The interface of plate.
In one of them preferred version, the top of the drainage channel section between described first well and the second well sets
There is the vertical interface inserting plate；Or in another selects scheme, described second well is located in terrace structure one
Inside the tread of subordinate's ladder, and the outlet of the drainage channel section between described first well and the second well exposes to
On step lateral wall, described outlet is the interface of laterally inserted plate.
In practice, described center well can be semicircle, circular, oval, rectangle or rhombus, or one
The most irregularly shaped, the length of described each drainage channel is equal or unequal, is as the criterion can realize testing goal of the present invention.Wherein
The present invention detects the preferred scheme of device: described first well is circular structure, and described drainage channel is around described
On half edge that first well is circular, each described drainage channel length is equal.
Further, described detection plate also includes the trough being located at detection zone one end.
Further, described detection device is medical disposable material, and detection plate surface uses the scraps of paper or acrylic material to prepare
Form.
In another scheme, described detection device also includes that upper cover, described upper cover cover above detection plate.Upper cover is deep
Degree is relatively big, is advisable can detection plate entirety be covered.
Advantages of the present invention and having the beneficial effects that:
The single centre multi-channel assembled detection device of the present invention, for microflow control technique, it is achieved each sample pipetting volume is equal,
" one-to-many " detection pattern, and it is applicable to the detection of various microbial biochemical, susceptibility detection etc.；Can be also used for thin after increasing upper cover
Born of the same parents cultivate and drug effect is cultivated.First well can contain more liquid (such as blood, urine or other Cell saps etc.), and liquid can
Add 50ul；At the step that drainage channel inner bottom surface is arranged so that when the liquid of the first well exceedes shoulder height, mean allocation
To each passage, thus realize mean allocation sample-adding amount.The capacity of the second well is then relevant to reactive tank volume, in using
Reactive tank volume being arranged to several specifications, and selects fixing specification according to service condition, the second well has solely
The effect of vertical sample-adding tune amount.Present in the detection device solution joint-detection of the present invention, fluid sample distribution is difficult to control to, liquid
Body sample flow is too fast causes the problems such as spill losses so that multinomial detection can be carried out by Simultaneous Stabilization, and can be read by machinery
Color changes and obtains testing result.
Accompanying drawing explanation
Fig. 1 is the structural representation that the present invention detects the first embodiment of plate；
Fig. 2 is the sectional view of Fig. 1 position A-A；
Fig. 3 is the structural representation that the present invention detects the second embodiment of plate；
Fig. 4 is the structural representation that the present invention detects the third embodiment of plate；
Fig. 5 is the structural representation that the present invention detects the 4th kind of embodiment of plate；
Fig. 6 is the axonometric chart that the present invention detects the 5th kind of embodiment of plate；
Fig. 7 is the front view that the present invention detects the 5th kind of embodiment of plate；
Fig. 8 is the sectional view of Fig. 7 position B-B；
Fig. 9 is the structural representation that the present invention detects the 6th kind of embodiment of plate；
Figure 10 is the schematic diagram that the present invention detects plate lid.
Detailed description of the invention
Below in conjunction with the accompanying drawings and embodiment, the detailed description of the invention of the present invention is further described.Following example are only
For technical scheme is clearly described, and can not limit the scope of the invention with this.
Single centre of the present invention multi-channel assembled detection device, including detection plate 1, described detection plate 1 is provided with at least one
Detection zone 2, described detection zone 2 includes being loaded plummer 3 and being located at multiple reactive tanks 4 of described sample-adding plummer 3 periphery；Institute
State sample-adding plummer 3 center or side is provided with the first well 30, hold to sample-adding for starting point with described first well 30
Microscope carrier 3 surrounding edge or opposite side week, described drainage channel 5 was connected with reactive tank 4 one_to_one corresponding along being extended with multiple drainage channel 5
Logical；Inner bottom surface along described drainage channel 5 is provided with some grades of steps 50.
Being additionally provided with the second well 31 on described sample-adding plummer 3, described second well 31 hangs down with described drainage channel 5
Directly it is connected, and described second well 31 is located at the outer end of high-order step.
Fig. 1 show the first embodiment of single centre of the present invention multi-channel assembled detection device, has a detection
District 2, Fig. 2 is the cross section view of this detection device.Wherein, some grades of steps 50 of the present invention add from first for being
Position, sample hole 30 is provided with some grades of steps along end in 3 weeks to sample-adding plummer, and the most described some grades of steps are to decline step by step or first
The step declined after rising；Most preferably first rise the step 50 declined afterwards, as shown in Figure 2.
It can be to have a detection zone 2 that the present invention detects the detection plate 1 of device, or for having two or more
The structure of detection zone 2.The present invention detects the reactive tank 4 of device and carries out around arrangement or center pair centered by sample-adding plummer 3
Claim arrangement；Or axle carries out axial symmetry arrangement centered by sample-adding plummer 3.Corresponding, described first well 30 cross section
Can be semicircle, circular, oval, rectangle or rhombus, or some are irregularly shaped, the length of described each passage 4 be equal
Or unequal, it is as the criterion so that testing goal of the present invention can be realized.Fig. 3 illustrates single centre of the present invention multi-channel assembled detection device
The second embodiment, its first well 30 is circular；Fig. 4 illustrates the of single centre of the present invention multi-channel assembled detection device
Three kinds of embodiments, its detection plate 1 has two detection zones 2；Fig. 5 illustrates single centre of the present invention multi-channel assembled detection device
4th kind of embodiment, its detection plate 1 has two detection zones 2, and the first well 30 cross section is rectangle and diamond configuration.
In a preferred embodiment of single centre of the present invention multi-channel assembled detection device, as shown in Fig. 6-8
Five embodiments, described first well 30 cross section is circular structure, and described drainage channel 5 is around described first well 30
On half circular edge, each described drainage channel 5 length is equal.Described sample-adding plummer 3 is by the first well 30 position
The terrace structure declined step by step to direction, drainage channel 5 exit position, ladder progression can be 2-6 level, preferably 3 grades ladders；
Said structure design makes to be loaded the structure that plummer 3 generally several semicolumn steps are formed.
This be embodied as inner in, the drainage channel shown in Fig. 8, between described first well 30 and the second well 31
5 segment marks are designated as first paragraph 51, and another section of drainage channel 5 segment mark of the second well 31 to drainage channel 5 end is designated as second segment
52；The step 50 of corresponding first paragraph 51 is the class outwards stepped up from the first well 30, and the platform of corresponding second segment 52
Rank 50 are the class outwards declined from inner side.Described first paragraph 51 is provided with and laterally or vertically inserts inserting of plate (not shown)
Interface 8；Concrete a kind of mode is to pass to above whole first paragraph 51 be loaded plummer 3 outer end, can the most vertically insert during use
Plugboard carries out passage obstruct, thus realizes the quantity to using drainage channel 5 and control.Certainly, the vertical grafting of the present invention is inserted
The mode of plate is not limited only to this, it is also possible to being to realize any technical scheme that passage intercepts, such as first paragraph 51 is pipeline
Structure, the sample-adding plummer 3 above this pipeline arranges a through hole, is used for inserting vertically moving plate, it is achieved intercept.
In the 5th embodiment, described detection plate 1 also includes the trough 6 being located at detection zone 2 one end；This trough 6
It is arranged on the one end not being surrounded with reactive tank 4.
In another preferred embodiment of single centre of the present invention multi-channel assembled detection device, as shown in Figure 9
Six embodiments, it is shown that the embodiment of the technical scheme of another kind of grafting plate.It is specially described second well 31 and is located at rank
In ladder structure inside the tread of subordinate's ladder, and the drainage channel 5 between described first well 30 and the second well 31
The outlet of section exposes on upper level ladder lateral wall, and described outlet is the interface 8 of laterally inserted plate；Can be direct during use
Transverse splicing plate carries out passage obstruct.
It is medical disposable material that the present invention detects device, and described detection plate 1 uses the scraps of paper or acrylic material to be prepared from.
Single centre of the present invention multi-channel assembled detection device also includes upper cover 10, and shown in Figure 10, described upper cover 10 covers
The top of detection zone 2；Upper cover 10 degree of depth is relatively big, is advisable can detection zone 2 entirety be covered.
The single centre multi-channel assembled detection device of the present invention can be used for microorganism, immune biochemical reaction detection, immunity
The multiple detections such as chromatography Multiple detection, cell cultivation and bacterial susceptibility detection.
Such as when microorganism, immune biochemical reaction detection, before use, reactive tank 4 is pre-loaded with for carrying out core
Acid amplified reaction, biochemical reaction, immunoreation or the material of other forms detection, or detect for same reaction formation
Different material, thus one detection plate on realize multiple application.Such as, to pass through nucleic acid amplification reaction on detection plate
Specific nucleic acid fragment (gene of such as certain particular person or the gene of pathogenic microorganism) in detection sample, can be anti-in difference
Answer that be pre-loaded with in groove 4 can be with the primer of different IPs acid fragment generation specific reaction in measuring samples and auxiliary element；To
By the predetermined substance in biochemical reaction detection sample or composition (such as blood glucose or triglyceride) on detection plate, can be not
With reactive tank 4 is pre-loaded with material and the auxiliary that can react with different material in measuring samples or composition generation specific biochemical
Composition；To detect the special component (such as certain antigen or antibody) in sample by immunoreation on detection plate, permissible
Differential responses groove 4 is pre-loaded with can with different material in measuring samples or the material of composition generation specific immune response and
Auxiliary element.In use, sample injector or conduit is used to be loaded to reactive tank 4 from the first well 30, drawing of setting table scalariform
Overflow after liquid exceedes step 50 height during circulation road 5 sample introduction, be evenly distributed to each passage, thus realize mean allocation sample-adding
Amount；Add set amount liquid also by the second well 31 during use and carry out the regulation and control that sample-adding amount is how many, also can pass through it
Add other reagent or liquid etc., to ensure that in reactive tank 4, liquid remains full of or carries out other reactions during whole reaction；
Last sentence read result, compares testing result with standard display result, it is judged that whether certain immunoreation occurs, and determines certain
Plant whether target checking matter exists.
And for example when bacterial susceptibility detects, different inoculums is added from the first well 30, respectively enters
In each reactive tank 4；Then add appropriate antibiotic by the second well 31, be configured to different drug level, and
The reactive tank 4 side records of values that labelling is corresponding respectively；Finally accessing detected strain, whether the antibacterial of observing response groove 4 gives birth to
Long, by break sensitive in multiple reactive tanks 4, drug resistance break and three holes of intermediary's break, it is judged that be drug resistance or sensitivity.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For Yuan, on the premise of without departing from the technology of the present invention principle, it is also possible to make some improvements and modifications, these improvements and modifications
Also should be regarded as protection scope of the present invention.
Claims (10)
1. a single centre multi-channel assembled detection device, including detection plate (1), it is characterised in that described detection plate (1) sets
Having the detection zone (2) of at least one, described detection zone (2) includes being loaded plummer (3) and being located at described sample-adding plummer (3) week
Multiple reactive tanks (4) on limit；Described sample-adding plummer (3) center or side are provided with the first well (30), with described
One well (30) is that starting point is extended with multiple drainage channel (5) to sample-adding plummer (3) surrounding edge or opposite side week edge, described
Drainage channel (5) is connected with reactive tank (4) one_to_one corresponding；Inner bottom surface along described drainage channel (5) is provided with some grades of steps
(50)。
2. single centre multi-channel assembled detection device as claimed in claim 1, it is characterised in that described some grades of steps
(50) it is to the decline step by step of edge, periphery or first to rise the step declined afterwards from the first well (30).
3. single centre multi-channel assembled detection device as claimed in claim 2, it is characterised in that described sample-adding plummer
(3) being additionally provided with the second well (31) on, described second well (31) is vertically connected logical with described drainage channel (5), and institute
State the second well (31) and be located at the outer end of high-order step.
4. the single centre multi-channel assembled detection device as according to any one of claim 1-3, it is characterised in that described instead
Groove (4) is answered to carry out centered by being loaded plummer (3) around arrangement or centrosymmetry arrangement；Or with sample-adding plummer (3) it is
Central shaft carries out axial symmetry arrangement.
5. single centre multi-channel assembled detection device as claimed in claim 3, it is characterised in that described sample-adding plummer
(3) for the terrace structure declined step by step to drainage channel (5) direction, exit position by the first well (30) position.
6. single centre multi-channel assembled detection device as claimed in claim 5, it is characterised in that described first well
(30) drainage channel (5) section between and the second well (31) is provided with the interface (8) laterally or vertically inserting plate.
7. single centre multi-channel assembled detection device as claimed in claim 6, it is characterised in that described first well
(30) drainage channel (5) section between and the second well (31) be arranged over vertically inserting the interface (8) of plate；
Or described second well (31) is located in terrace structure inside the tread of subordinate's ladder, and described first well
(30) outlet of drainage channel (5) section between and the second well (31) exposes on upper level ladder lateral wall, described in go out
Mouth is the interface (8) of laterally inserted plate.
8. single centre multi-channel assembled detection device as claimed in claim 1, it is characterised in that described first well
(30) be circular structure, described drainage channel (5) on half edge that described first well (30) is circular, each described in draw
Circulation road (5) length is equal.
9. single centre multi-channel assembled detection device as claimed in claim 1, it is characterised in that described detection plate (1) is also
Including the trough (6) being located at detection zone (2) one end.
10. single centre multi-channel assembled detection device as claimed in claim 1, it is characterised in that described detection plate (1) table
Face uses the scraps of paper or acrylic material to be prepared from, and described detection device also includes that upper cover (7), described upper cover (7) cover in detection
The top in district (2).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610798408.7A CN106222081B (en) | 2016-08-31 | 2016-08-31 | The multi-channel assembled detection device of single centre |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610798408.7A CN106222081B (en) | 2016-08-31 | 2016-08-31 | The multi-channel assembled detection device of single centre |
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| CN106222081A true CN106222081A (en) | 2016-12-14 |
| CN106222081B CN106222081B (en) | 2019-01-15 |
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| CN109669036A (en) * | 2019-02-28 | 2019-04-23 | 深圳市易瑞生物技术股份有限公司 | A kind of push type multichannel chromatographic apparatus |
| CN109709158A (en) * | 2017-10-25 | 2019-05-03 | 天津蓝蛇伟业医疗器械有限公司 | A kind of high-efficiency multi-function blood testing strips detector |
| CN110018147A (en) * | 2019-05-16 | 2019-07-16 | 深圳市英宝硕科技有限公司 | Bioaerosol collects identification disk |
| CN110579518A (en) * | 2019-09-12 | 2019-12-17 | 江南大学 | Tea polyphenol electrochemical detection device based on vertical mixing micro-fluidic chip |
| CN113777099A (en) * | 2021-07-28 | 2021-12-10 | 河南省中医院（河南中医药大学第二附属医院） | Quintuplet test for rapidly detecting TORCHIgM antibody |
| CN114798020A (en) * | 2022-03-28 | 2022-07-29 | 四川成电医联科技咨询有限公司 | Multi-channel electrochemical micro-fluidic test card and manufacturing method thereof |
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| CN110579518A (en) * | 2019-09-12 | 2019-12-17 | 江南大学 | Tea polyphenol electrochemical detection device based on vertical mixing micro-fluidic chip |
| CN113777099A (en) * | 2021-07-28 | 2021-12-10 | 河南省中医院（河南中医药大学第二附属医院） | Quintuplet test for rapidly detecting TORCHIgM antibody |
| CN114798020A (en) * | 2022-03-28 | 2022-07-29 | 四川成电医联科技咨询有限公司 | Multi-channel electrochemical micro-fluidic test card and manufacturing method thereof |
| CN114798020B (en) * | 2022-03-28 | 2024-07-09 | 四川成电医联科技咨询有限公司 | Multichannel electrochemical microfluidic test card and manufacturing method thereof |
| CN117825697A (en) * | 2024-03-01 | 2024-04-05 | 中国科学院过程工程研究所 | Hazard factor detection reagent card and detection method |
| CN117825697B (en) * | 2024-03-01 | 2024-05-07 | 中国科学院过程工程研究所 | Hazard factor detection reagent card and detection method |
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