CN106222081B - The multi-channel assembled detection device of single centre - Google Patents
The multi-channel assembled detection device of single centre Download PDFInfo
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- CN106222081B CN106222081B CN201610798408.7A CN201610798408A CN106222081B CN 106222081 B CN106222081 B CN 106222081B CN 201610798408 A CN201610798408 A CN 201610798408A CN 106222081 B CN106222081 B CN 106222081B
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- well
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
Abstract
The present invention relates to a kind of multi-channel assembled detection device of single centre, including detection plate, the detection plate is equipped with the detection zone of at least one, and the detection zone includes being loaded plummer and multiple reactive tanks set on the sample-adding plummer periphery；The sample-adding plummer center or side are equipped with the first well, are connected to sample-adding plummer surrounding edge or other side week along multiple drainage channels, the drainage channel is extended with reactive tank one-to-one correspondence using first well as starting point；Inner bottom surface along the drainage channel is equipped with several grades of steps.The device is microflow control technique, makes each sample pipetting volume equal；And it realizes and the detection pattern that one-time detection determines plurality of target detectable substance is carried out to a sample.
Description
Technical field
The present invention relates to biochemical analysis apparatus fields, and in particular to a kind of multi-channel assembled detection device of single centre.
Background technique
Current detection reagent most of on the market is all single index detection, however, with biomedical detection technique
Development, various emerging, valuable Testing index are constantly exploited, the testing result data sheet one of single index, no
The case where enough diseases of reflection comprehensively, the detection of many index is particularly important.
For above situation, which gradually improves single index monitoring device, such as patent application document
The novel multinomial joint inspection test paper of one kind disclosed in 200520128615.9, each first monitor shares a sample application zone in multinomial structure,
It only need to be once loaded in sample-adding, operation is more easy, however the design of the sample application zone of the structure is too simple, is not able to achieve sample
Respectively, sample distribution is non-uniform between every detection reagent occurring due to the difference of test strips water absorbing properties in actual use
Situation eventually leads to the deviation of testing result.A kind of for another example multi objective disk disclosed in patent application document 201520435678.2
Formula dot immuno gold filtration assay detection device, including bottom plate, cover board, water suction washer and carrier film, the bottom plate center are equipped with center
Sample application zone is equipped with more than one fan-shaped reaction zone around the center sample application zone, while the bottom plate is additionally provided with annular slot；Institute
Annular water suction washer is stated to be placed in the annular slot；The carrier film is placed in the center sample application zone and the fan-shaped reaction zone
It is interior, it is adjacent with the annular water suction washer；The cover board pushes down the carrier film and the water suction washer, and the cover plate lid is in institute
It states on bottom plate；The device once can detecte multiple indexs, simple to operate quick, have multi-channel parallelization operation excellent
Gesture.But the device can not control simultaneously center sample-adding amount and each sample pipetting volume amount.
Summary of the invention
The purpose of the present invention is to overcome the defects in the prior art, designs a kind of multi-channel assembled detection dress of single centre
It sets, is microflow control technique, makes each sample pipetting volume equal；Realize that carrying out one-time detection to a sample determines that plurality of target detects
The detection pattern of object solves the distribution unevenness of fluid sample present in joint-detection, and fluid sample circulation is too fast to cause overflow to damage
The problems such as mistake, so that multinomial detection can stable progress simultaneously.
To achieve the above object, the technical scheme adopted by the invention is that a kind of multi-channel assembled detection dress of single centre
It sets, including detection plate, the detection plate is equipped with the detection zone of at least one, and the detection zone includes being loaded plummer and set on institute
State multiple reactive tanks on sample-adding plummer periphery；The sample-adding plummer center or side are equipped with the first well, with institute
Stating the first well is starting point to plummer surrounding edge or other side week is loaded along multiple drainage channels are extended with, and the drainage is logical
Road is connected with reactive tank one-to-one correspondence；Inner bottom surface along the drainage channel is equipped with several grades of steps.
In further preferred embodiment, several grades of steps be decline step by step from the first well to outer periphery or first on
The step declined after rising.
Further preferred embodiment is that the second well is additionally provided on the sample-adding plummer, second well and
The drainage channel be vertically connected it is logical, and second well be set to highest stage rank outer end, can avoid the second well
The phenomenon that liquid of addition flows backward to the first well.
Further, the reactive tank carries out surrounding arrangement or central symmetry arrangement centered on being loaded plummer；Or
Axial symmetry arrangement is carried out as center axis to be loaded plummer.
Further, several grades of steps are to be equipped with several grades along end from the first sample-adding hole site to sample-adding plummer week
Step.
Further, the drainage channel section between first well and the second well is equipped with and is laterally or vertically inserted into
The interface of plate.
In one of preferred embodiment, set above the drainage channel section between first well and the second well
There is the interface of vertical insertion plate；Or selected in scheme at another, second well is set to one in terrace structure
On the inside of the tread of junior's ladder, and the outlet of the drainage channel section between first well and the second well exposes to
On step lateral wall, the outlet is the interface of laterally inserted plate.
In practice, the center well can be semicircle, round, ellipse, rectangle or diamond shape, Huo Zheyi
A little irregular shapes, the equal length or unequal of each drainage channel are subject to and testing goal of the present invention can be achieved.Wherein
The preferred scheme of detection device of the present invention is: first well is circular structure, and the drainage channel is around described
On circular half edge of first well, each drainage channel equal length.
Further, the detection plate further includes set on the trough of detection zone one end.
Further, the detection device is medical disposable material, and detection plate surface is prepared using the scraps of paper or acrylic material
It forms.
In another scheme, the detection device further includes upper cover, and the upper cover is covered in the top of detection plate.Upper cover is deep
It spends larger, is advisable with that can will test plate and integrally cover.
The advantages and beneficial effects of the present invention are:
The multi-channel assembled detection device of single centre of the invention is microflow control technique, realizes that each sample pipetting volume is equal,
" one-to-many " detection pattern, and be suitable for the detection of various microbial biochemicals, susceptibility detection etc.；It can be also used for carefully after increasing upper cover
Born of the same parents' culture and drug effect culture.First well can contain more liquid (such as blood, urine or other cell liquid), and liquid can
Add 50ul；In the step of drainage channel inner bottom surface setting, when so that the liquid of the first well being more than step height, mean allocation
To each channel, to realize mean allocation sample-adding amount.And the capacity of the second well is then related to reactive tank volume, in use
Reactive tank volume is arranged to several specifications, and fixed specification is selected according to service condition, the second well has only
The effect of vertical sample-adding tune amount.Detection device of the invention solves the distribution of fluid sample present in joint-detection and is difficult to control, liquid
Body sample flow too fast the problems such as causing spill losses so that multinomial detection can stable progress simultaneously, and mechanical read can be passed through
Color change and obtain testing result.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the first embodiment of detection plate of the present invention；
Fig. 2 is the cross-sectional view of the position Fig. 1 A-A；
Fig. 3 is the structural schematic diagram of second of embodiment of detection plate of the present invention；
Fig. 4 is the structural schematic diagram of the third embodiment of detection plate of the present invention；
Fig. 5 is the structural schematic diagram of the 4th kind of embodiment of detection plate of the present invention；
Fig. 6 is the perspective view of the 5th kind of embodiment of detection plate of the present invention；
Fig. 7 is the main view of the 5th kind of embodiment of detection plate of the present invention；
Fig. 8 is the cross-sectional view of the position Fig. 7 B-B；
Fig. 9 is the structural schematic diagram of the 6th kind of embodiment of detection plate of the present invention；
Figure 10 is the schematic diagram that detection plate of the present invention covers lid.
Specific embodiment
With reference to the accompanying drawings and examples, further description of the specific embodiments of the present invention.Following embodiment is only
For clearly illustrating technical solution of the present invention, and not intended to limit the protection scope of the present invention.
The multi-channel assembled detection device of single centre of the present invention, including detection plate 1, the detection plate 1 are equipped at least one
Detection zone 2, the detection zone 2 include sample-adding plummer 3 and set on it is described sample-adding 3 periphery of plummer multiple reactive tanks 4；Institute
It states sample-adding 3 center of plummer or side and is equipped with the first well 30, be that starting point is held to sample-adding with first well 30
3 surrounding of microscope carrier edge or other side week are connected along multiple drainage channels 5, the drainage channel 5 is extended with the one-to-one correspondence of reactive tank 4
It is logical；Inner bottom surface along the drainage channel 5 is equipped with several grades of steps 50.
The second well 31 is additionally provided on the sample-adding plummer 3, second well 31 hangs down with the drainage channel 5
Directly it is connected, and second well 31 is set to the outer end of highest stage rank.
Fig. 1 show the first embodiment of the multi-channel assembled detection device of single centre of the present invention, has a detection
Area 2, Fig. 2 are the cross section view of the detection device.Wherein, several grades of steps 50 of the present invention are that can be to add from first
30 position of sample hole was equipped with several grades of steps along end to sample-adding plummer 3 weeks, and the preferably described several grades of steps are decline step by step or first
The step declined after rising；Most preferably first rise the step 50 declined afterwards, as shown in Figure 2.
The detection plate 1 of detection device of the present invention can be for a detection zone 2, or for two or more
The construction of detection zone 2.The reactive tank 4 of detection device of the present invention carries out surrounding arrangement or center pair centered on being loaded plummer 3
Claim arrangement；Or axial symmetry arrangement is carried out to be loaded plummer 3 as center axis.It is corresponding, 30 cross section of the first well
It can be semicircle, round, ellipse, rectangle or diamond shape or some irregular shapes, the equal length in each channel 4
Or it is unequal, it is subject to and testing goal of the present invention can be achieved.Fig. 3 shows the multi-channel assembled detection device of single centre of the present invention
Second of embodiment, the first well 30 are circle；Fig. 4 shows the of the multi-channel assembled detection device of single centre of the present invention
Three kinds of embodiments, there are two detection zones 2 for the tool of detection plate 1；Fig. 5 shows the multi-channel assembled detection device of single centre of the present invention
4th kind of embodiment, there are two detection zones 2 for the tool of detection plate 1, and 30 cross section of the first well is rectangle and diamond configuration.
In a preferred embodiment of the multi-channel assembled detection device of single centre of the present invention, as shown in Fig. 6-8
Five embodiments, 30 cross section of the first well are circular structure, and the drainage channel 5 is around first well 30
On circular half edge, each 5 equal length of drainage channel.The sample-adding plummer 3 is by 30 position of the first well
The terrace structure declined step by step to 5 outlet port direction of drainage channel, ladder series can be 2-6 grades, preferably 3 grades of ladders；
Said structure design to be loaded the construction that generally several semicolumn steps of plummer 3 are formed.
Drainage channel in the specific implementation, shown in Fig. 8, between first well 30 and the second well 31
5 segment marks are denoted as first segment 51, and another section of 5 segment mark of drainage channel of the second well 31 to 5 end of drainage channel is denoted as second segment
52；The step 50 of corresponding first segment 51 is the class stepped up outward from the first well 30, and corresponds to the platform of second segment 52
Rank 50 is the class declined outward from inside.The first segment 51, which is equipped with, is laterally or vertically inserted into inserting for plate (not shown)
Interface 8；A kind of specific mode is that sample-adding 3 outer end of plummer is passed to above entire first segment 51, and when use directly can vertically insert
Plugboard carries out channel barrier, controls to realize the quantity for using drainage channel 5.Certainly, vertical grafting of the invention is inserted
The mode of plate is not limited only to this, can also be any technical solution for being able to achieve channel barrier, such as first segment 51 is pipeline
It constructs, a through-hole is set on the sample-adding plummer 3 above the pipeline, for being inserted into vertically moving plate, realize barrier.
In the 5th embodiment, the detection plate 1 further includes set on the trough 6 of 2 one end of detection zone；The trough 6
The one end for not being surrounded with reactive tank 4 is set.
In another preferred embodiment of the multi-channel assembled detection device of single centre of the present invention, as shown in Figure 9
Six embodiments show the embodiment of the technical solution of another grafting plate.Specially described second well 31 is set to rank
In ladder construction on the inside of the tread of junior's ladder, and the drainage channel 5 between first well 30 and the second well 31
The outlet of section exposes on upper level ladder lateral wall, and the outlet is the interface 8 of laterally inserted plate；It can be direct when use
Transverse splicing plate carries out channel barrier.
Detection device of the present invention is medical disposable material, and the detection plate 1 is prepared using the scraps of paper or acrylic material.
The multi-channel assembled detection device of single centre of the present invention further includes upper cover 10, and shown in Figure 10, the upper cover 10 is covered
The top of detection zone 2；10 depth of upper cover is larger, is advisable with that can will test whole cover in area 2.
The multi-channel assembled detection device of single centre of the invention can be used for microorganism, immune biochemical reaction detection, be immunized
Chromatograph a variety of detections such as Multiple detection, cell culture and bacterial susceptibility detection.
Such as when for microorganism, immune biochemical reaction detection, before use, reactive tank 4 is pre-loaded with for carrying out core
Sour amplified reaction, biochemical reaction, the immune response substance that perhaps other forms detect are detected for same reaction formation
Different material, to realize a variety of applications in a kind of detection plate.For example, to pass through nucleic acid amplification reaction in detection plate
In test sample specific nucleic acid fragment (such as some particular person gene or pathogenic microorganism gene), can be different anti-
Answer the primer and auxiliary element for being pre-loaded in slot 4 and specific reaction can occurring from nucleic acid fragments different in measuring samples；To
It, can be not by the predetermined substance or ingredient (such as blood glucose or triglyceride) in biochemical reaction test sample in detection plate
The substance and auxiliary that specific biochemical reacts can occur with different material in measuring samples or ingredient with being pre-loaded in reactive tank 4
Ingredient；To in detection plate by the special component (such as certain antigen or antibody) in immune response test sample, can be with
Be pre-loaded in differential responses slot 4 substance that specific immune response can occur with different material in measuring samples or ingredient and
Auxiliary element.When in use, it is loaded using sample injector or conduit from the first well 30 to reactive tank 4, step-like draw is set
Liquid is evenly distributed to each channel more than overflowing after 50 height of step when 5 sample introduction of circulation road, to realize that mean allocation is loaded
Amount；Also sample-adding can be carried out by the second well 31 addition setting quantity of fluid during use and measure how many regulations, can also pass through it
Other reagents or liquid etc. is added, to guarantee that liquid remains full of or carry out other reactions in entirely reaction present invention slot 4；
Last interpretation result will test result and be compared with standard shows result, judge whether certain immune response occurs, and determine certain
Kind target checking matter whether there is.
When for another example for bacterial susceptibility detection, different inoculums is added from the first well 30, is respectively enterd
In each reactive tank 4；Then suitable antibiotic is added by the second well 31, is configured to different drug concentrations, and
4 side of reactive tank marks corresponding records of values respectively；It finally accesses and is detected strain, whether the bacterium of observing response slot 4 gives birth to
Long, by three holes of break sensitive in multiple reactive tanks 4, drug resistance break and intermediary's break, judgement is drug resistance or sensitivity.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the technical principles of the invention, several improvements and modifications can also be made, these improvements and modifications
Also it should be regarded as protection scope of the present invention.
Claims (8)
1. a kind of multi-channel assembled detection device of single centre, including detection plate (1), which is characterized in that the detection plate (1) sets
There is the detection zone (2) of at least one, the detection zone (2) includes being loaded plummer (3) and set on sample-adding plummer (3) week
Multiple reactive tanks (4) on side；The sample-adding plummer (3) center or side are equipped with the first well (30), with described the
One well (30) is that starting point is extended with multiple drainage channels (5) to sample-adding plummer (3) surrounding edge or other side week edge, described
Drainage channel (5) is corresponded with reactive tank (4) to be connected；Inner bottom surface along the drainage channel (5) is equipped with several grades of steps
(50)；Several grades of steps (50) are to decline step by step from the first well (30) to outer periphery or first rise the platform declined afterwards
Rank；
It is additionally provided with the second well (31) on the sample-adding plummer (3), second well (31) and the drainage channel
(5) be vertically connected it is logical, and second well (31) be set to highest stage rank outer end.
2. the multi-channel assembled detection device of single centre as described in claim 1, which is characterized in that the reactive tank (4) with
It carries out surrounding arrangement or central symmetry arrangement centered on sample-adding plummer (3)；Or to be loaded plummer (3) as the progress of center axis
Axial symmetry arrangement.
3. the multi-channel assembled detection device of single centre as described in claim 1, which is characterized in that the sample-adding plummer
(3) terrace structure to decline step by step from the first well (30) position to drainage channel (5) outlet port direction.
4. the multi-channel assembled detection device of single centre as claimed in claim 3, which is characterized in that first well
(30) drainage channel (5) section between the second well (31) is equipped with the interface (8) for being laterally or vertically inserted into plate.
5. the multi-channel assembled detection device of single centre as claimed in claim 4, which is characterized in that first well
(30) top of drainage channel (5) section between the second well (31) is equipped with the interface (8) of vertical insertion plate；
Or second well (31) is set in terrace structure on the inside of the tread of junior's ladder, and first well
(30) outlet of drainage channel (5) section between the second well (31) exposes on upper level ladder lateral wall, it is described go out
Mouth is the interface (8) of laterally inserted plate.
6. the multi-channel assembled detection device of single centre as described in claim 1, which is characterized in that first well
It (30) is circular structure, the drainage channel (5) is each described to draw on circular half edge of the first well (30)
Circulation road (5) equal length.
7. the multi-channel assembled detection device of single centre as described in claim 1, which is characterized in that the detection plate (1) is also
Trough (6) including being set to detection zone (2) one end.
8. the multi-channel assembled detection device of single centre as described in claim 1, which is characterized in that detection plate (1) table
Face is prepared using the scraps of paper or acrylic material, and the detection device further includes upper cover (7), and the upper cover (7), which is covered, to be detected
The top in area (2).
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| CN201610798408.7A CN106222081B (en) | 2016-08-31 | 2016-08-31 | The multi-channel assembled detection device of single centre |
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| CN109709158A (en) * | 2017-10-25 | 2019-05-03 | 天津蓝蛇伟业医疗器械有限公司 | A kind of high-efficiency multi-function blood testing strips detector |
| CN109669036B (en) * | 2019-02-28 | 2024-03-15 | 深圳市易瑞生物技术股份有限公司 | Push type multichannel chromatographic device |
| CN110018147B (en) * | 2019-05-16 | 2024-03-08 | 深圳市英宝硕科技有限公司 | Biological aerosol collecting and identifying disc |
| CN110579518B (en) * | 2019-09-12 | 2020-07-07 | 江南大学 | Tea polyphenol electrochemical detection device based on vertical mixing micro-fluidic chip |
| CN113777099A (en) * | 2021-07-28 | 2021-12-10 | 河南省中医院（河南中医药大学第二附属医院） | Quintuplet test for rapidly detecting TORCHIgM antibody |
| CN114798020A (en) * | 2022-03-28 | 2022-07-29 | 四川成电医联科技咨询有限公司 | Multi-channel electrochemical micro-fluidic test card and manufacturing method thereof |
| CN117825697A (en) * | 2024-03-01 | 2024-04-05 | 中国科学院过程工程研究所 | Hazard factor detection reagent card and detection method |
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