US20230349867A1 - Chromatography Apparatus - Google Patents
Chromatography Apparatus Download PDFInfo
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- US20230349867A1 US20230349867A1 US18/350,588 US202318350588A US2023349867A1 US 20230349867 A1 US20230349867 A1 US 20230349867A1 US 202318350588 A US202318350588 A US 202318350588A US 2023349867 A1 US2023349867 A1 US 2023349867A1
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- 238000004587 chromatography analysis Methods 0.000 title claims abstract description 73
- 238000000034 method Methods 0.000 claims abstract description 37
- 230000008569 process Effects 0.000 claims abstract description 18
- 239000012530 fluid Substances 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 14
- 238000012856 packing Methods 0.000 claims description 14
- 238000004140 cleaning Methods 0.000 claims description 4
- 238000004891 communication Methods 0.000 claims description 4
- 230000006835 compression Effects 0.000 claims description 4
- 238000007906 compression Methods 0.000 claims description 4
- 239000002699 waste material Substances 0.000 claims description 4
- 230000008859 change Effects 0.000 claims description 3
- 238000013375 chromatographic separation Methods 0.000 claims description 2
- 230000031700 light absorption Effects 0.000 claims description 2
- 238000003860 storage Methods 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 238000011194 good manufacturing practice Methods 0.000 description 6
- 230000008901 benefit Effects 0.000 description 5
- 239000012501 chromatography medium Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- 238000012800 visualization Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000872 buffer Substances 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 238000004811 liquid chromatography Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 230000004913 activation Effects 0.000 description 1
- 238000010364 biochemical engineering Methods 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000003749 cleanliness Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
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- 238000010828 elution Methods 0.000 description 1
- 229920002457 flexible plastic Polymers 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
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- 238000000275 quality assurance Methods 0.000 description 1
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Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/44—Flow patterns using recycling of the fraction to be distributed
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6052—Construction of the column body
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8696—Details of Software
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
- G01N2030/326—Control of physical parameters of the fluid carrier of pressure or speed pumps
Definitions
- the present invention relates to liquid chromatography apparatus, in particular but not exclusively to a laboratory or ‘benchtop’ size instrument of a versatile nature allowing convenient reconfiguration for different chromatographic procedures and convenient automated use.
- the invention extends to methods for operating such instruments.
- Liquid chromatography is a well-known procedure for separating mixtures of molecules, for example separating proteins in liquid samples.
- the proteins may typically be suspended in a fluid, and driven through a chromatography separation medium along with a buffer solution.
- the various sample molecules of the mixture travel at different speeds through a chromatography medium, causing them to separate.
- This separation may be completed by a fractionation step where the mobile phase may be directed to different containers, e.g. by an outlet valve of the chromatography apparatus.
- continuous chromatography two or more identical columns are connected in an arrangement that allows columns to be operated in series and/or in parallel, depending on the method requirements. Thus, all columns can be run in principle simultaneously, but in different stages. The procedure may be repeated, such that each column is loaded/packed, eluted, and regenerated several times in the process.
- ‘conventional’ chromatography wherein a single chromatography cycle is based on several consecutive steps, such as loading, wash, elution and regeneration, in continuous chromatography based on multiple identical columns all these steps occur simultaneously but on different columns.
- Continuous chromatography operation results in a better utilization of chromatography media compositions, reduced processing time and reduced buffer fluid requirements, all of which benefits process economy.
- Continuous chromatography is sometimes denoted simulated moving bed (SMB) chromatography.
- each column may be loaded/packed, eluted, cleaned, and regenerated several times in the process.
- An essential factor for a reliable continuous chromatography process is the quality of the columns used, and more specifically the similarity or even density of media in each column. If the columns are not identical, the theoretical calculations will not be correct, and it will become difficult to design an efficient and robust continuous chromatography process.
- the loading/packing of a column with chromatography media composition is very complex in order to obtain repeatable results. Even small differences in the number of plates or other packing properties can have a huge effect on the end result.
- a problem with conventional solutions is that performing continuous chromatography is a cumbersome, complex and time consuming operation. Often the process must be interrupted to perform reconnection of fluid couplings/tubes, to perform packing of columns or to load a pre packed column, to perform cleaning operations etc.
- an object of the present invention is to provide a chromatography instrument with one or more of: an increased functionality, for example able to operate in conventional batch chromatography as well as continuous chromatography; is useable across a wider range of applications; does not have a substantially increased overall size or manufacturing cost; and is simple to operate.
- GMP Good Manufacturing Practice
- the standards are easier to achieve with the proposed apparatus, for example where fluid paths in the instrument have, in one configuration at least, a continuous flow path with no substantive stagnant portions, thereby providing complete cleaning without the need to break down the fluid conduits.
- Embodiments of the proposed instrument provide a sanitary small-scale chromatography instrument suitable for both GMP and non-GMP work. Functionally wide flow and pressure ranges of the instrument makes it fit for both production of technical batches and scale-up studies as well as small-scale production of GMP-grade material. The high accuracy and flow range of the pumps enables precise gradient formation, covering a large range of chromatography column sizes and more repeatable results.
- a modular construction provides increased functionality, for different uses.
- Interactive control software allows changes to be made in real-time and unexpected deviations to be quickly identified.
- the small, bench-top size frees up lab space.
- the instrument allows in-situ column packaging, i.e. the ability to compress chromatography media in the column, or each column where two or more columns are used, whilst being connected to the instrument, and without having to then disconnect any fluid conduits prior to performing chromatographic procedures.
- chromatography instrument as claimed herein having features set out the in independent apparatus claims, are further preferred features set out in the dependent apparatus claims.
- FIG. 1 shows a pictorial view of a new chromatography apparatus
- FIG. 2 shows schematic representation of the apparatus shown in FIG. 1 ;
- FIGS. 3 , 4 and 5 show configurations of the apparatus shown in the previous Figures.
- FIG. 1 sows a chromatography apparatus 100 according to an aspect of the invention.
- the apparatus includes, but it not limited to, individual modules 1 to 25 as listed below, at least some of which are demountable from an apertured front panel 120 of a housing 110 of the apparatus 100 and mounted thereon in one generally vertical plane, such that the liquid connections required between modules can be made only at the front face 120 .
- the demountable modules have no more than two standard sizes which can, if needed, be repositioned on the panel 120 to suit a different procedure.
- Each module has a serial bus communication connection and power connection so that its physical position is immaterial to a controller for example located in the housing 110 , or located remotely. Thereby, the modules can be regarded as modular and thereby repositionable and/or interchangeable.
- the chromatography apparatus shown in FIG. 1 has the following module modules:
- Modules can be omitted or repositioned as explained above. It will be apparent that some modules can be replaced with other modules or the space left by an omitted module can be filled with a blanking plate (see e.g. 26 FIG. 4 ). More than one of the same numbered modules can be used where necessary.
- Fluid interconnections between the fluid manipulating modules of the apparatus i.e. all the modules listed above except modules 1 , 15 and 23 , and external modules for example sample input reservoirs, buffer fluid reservoirs, chromatograph column(s) and fraction collection equipment, all not shown in FIG. 1 , are made via fluid conduits in this case in the form of flexible plastics tubing, which can be readily coupled and uncoupled to corresponding ports of the fluid manipulating modules, in any desired configuration, for example using a coupling as disclosed in a related application GB1710279 filed 28 Jun. 2017 incorporated herein by reference.
- FIG. 2 shows one possible liquid interconnection configuration between the main modular modules of the chromatography apparatus, connected in this case to two chromatography columns 200 and 300 , although the apparatus allows any workable interconnection between modules and additional parts such as multiple columns, and liquid reservoirs.
- Reconfigurable liquid interconnections are denoted by short chain dotted lines 80 .
- the column valve 8 At the heart of the apparatus 100 is the column valve 8 , which in this case has a construction as disclosed in our co-pending patent application filed on the same day as this application, and having the title ‘VALVE UNIT FOR A CHROMATOGRAPHY APPARATUS’ and is incorporated herein by reference.
- the value unit 8 provides multiple switching of flow for allowing flow in one or both 20 columns 200 / 300 in either direction (up or down in the drawing). The user can select upflow or downflow, or select to bypass one or both columns. The flow can be directed to waste or to the next component in the flow path.
- the columns can also be connected in series.
- the Column valve includes pre-column and post-column pressure sensors.
- valve has a port 150 which can be used to change the volume of hydraulic cylinders 210 are 310 which are part of the columns 200 and 300 , for example to provide compression of the columns' contents, also known as column packing. That packing procedure can be automated. With such a system column diameters of between about 25 and 250 mm have been found to be packable in this way.
- the columns can be pre-packed, but rinsed and re-consolidated with the aid of pressure sensors in the value unit 8 measuring back-pressure resulting from pressure within the columns and in accordance with to known protocols, for example as described in WO200704549 1, which disclosure is incorporated herein by reference.
- the remaining system 100 comprises:
- FIGS. 3 , 4 and 5 show the system connected with tubing for various configurations, where only some of the modules referenced in FIG. 1 remain in place in these figures, and the apertures left by removed modules are blanked off with blanking plates 26 , screwed into place over the aperture to prevent accidental liquid ingress into the housing 110 .
- FIG. 3 a system 100 ′ with a configuration of modules suitable for regulated environments where systems are custom-built in a factory. The system is delivered mounted, calibrated, and performance tested and suitable for work in GMP environments.
- FIG. 4 shows one system with some modules removed
- FIG. 5 shows a system 100 ′′′ with more modules in place, similar to FIG. 1 , and showing typical tubular interconnections 80 .
- modules are easily removed or added to the system and installation finalized through a one-click activation in software which can recognize each module.
- the software can provide comprehensive and customizable operational control as well as pre-emptive maintenance.
- input-output communication modules can be used to interface with analog and/or digital external sensors or other equipment such as automatic fraction collecting devices.
- the wide flow rate and pressure ranges enables more than 40-fold scaling in the range 25 to 250 mm internal diameter columns. This wide range makes the apparatus suitable to bridge the transition into GMP environments.
- the packing (and re-packing) of chromatography columns, using the system described above is controllable fully by the controller 600 initiated by the control panel 1 .
- the controller 600 is able to drive the a display screen 30 ( FIG. 2 ) to aid visualisation of the packing process and progress.
- the control software includes an accessible column packing record. Columns packing records can therefore be defined, created, and updated from the software for traceability and quality assurance purposes. In addition, the record can be used to monitor column performance and provide statistics for usage, separation performance, and packing intervals.
- the display screen can provide a process visualization which quickly gives an operator an overview of the system's function, progress through operational steps and alarms, only providing the desired amount of information at each step.
- the active flow path is always displayed in the process visualisation to minimize user errors. Real time changes can be made by selecting the appropriate process on the visualization screen, e.g. selecting or dragging icons on the screen.
- Control, graphical interfaces are provided for specific sections, such as the column valve 8 .
- Preprogrammed steps are employed but these can be modified and saved as user-defined steps for added customization.
- the system chassis 110 is flat or curved without joints, gaps or significant concavities, other than at the edges of the faces, which makes it easy to wipe down and reduces the chance of dust and liquid trapping.
- the pH monitor 2 has in-line calibration and the column valve 8 provides in-process column packing, so a closed flow path through operations can be employed, meaning that no breaks in the fluid path need be made throughout one or more chromatography column packing/regeneration stages and throughout the separation operation,
Abstract
Disclosed is a chromatography system (100) comprising: plural modules (1-25) including at least one pump and a column valve unit (8) connectable to plural chromatography columns; and a controller (600), the controller being operable to control the or each pump and the column valve to perform different chromatographic processes, including chromatography employing just one column, as well as chromatography employing just one column, as well as chromatography employing two or more columns by selective valve opening in said unit. The system includes a housing (110) into which the plural modules (1-25) are interchangeably mountable in apertures of one generally vertical face of housing, the modules are adapted for selective fluidic interconnection by tubing substantially at said one face such that in use the modules and tubing occupy a generally vertically extending volume to minimize the footprint of the system.
Description
- This application is a continuation of U.S. application Ser. No. 16/647,983, filed Mar. 17, 2020, which claims the priority benefit of PCT/EP2018/075693, filed Sep. 21, 2018, which claims the priority benefit of Great Britain Application No. 1715403.0, filed Sep. 22, 2017, the entire contents of which are incorporated herein by reference.
- The present invention relates to liquid chromatography apparatus, in particular but not exclusively to a laboratory or ‘benchtop’ size instrument of a versatile nature allowing convenient reconfiguration for different chromatographic procedures and convenient automated use. The invention extends to methods for operating such instruments.
- Liquid chromatography is a well-known procedure for separating mixtures of molecules, for example separating proteins in liquid samples. The proteins may typically be suspended in a fluid, and driven through a chromatography separation medium along with a buffer solution. The various sample molecules of the mixture travel at different speeds through a chromatography medium, causing them to separate. This separation may be completed by a fractionation step where the mobile phase may be directed to different containers, e.g. by an outlet valve of the chromatography apparatus.
- In some applications, e.g. in the biopharmaceutical field, recent advancements in genetic engineering and cell culture technology have driven expression levels higher than ever, putting a considerable burden on down-stream purification, especially the fractionation step. While the introduction of new chromatography media compositions significantly improves the efficiency of a process based on a conventional fixed bed chromatography, additional gains can be achieved by operating in a continuous manner. The latter is especially appealing when continuous bioreactors, such as those operated in perfusion mode, are employed.
- In continuous chromatography, two or more identical columns are connected in an arrangement that allows columns to be operated in series and/or in parallel, depending on the method requirements. Thus, all columns can be run in principle simultaneously, but in different stages. The procedure may be repeated, such that each column is loaded/packed, eluted, and regenerated several times in the process. Compared to ‘conventional’ chromatography, wherein a single chromatography cycle is based on several consecutive steps, such as loading, wash, elution and regeneration, in continuous chromatography based on multiple identical columns all these steps occur simultaneously but on different columns. Continuous chromatography operation results in a better utilization of chromatography media compositions, reduced processing time and reduced buffer fluid requirements, all of which benefits process economy. Continuous chromatography is sometimes denoted simulated moving bed (SMB) chromatography.
- As previously mentioned each column may be loaded/packed, eluted, cleaned, and regenerated several times in the process. An essential factor for a reliable continuous chromatography process is the quality of the columns used, and more specifically the similarity or even density of media in each column. If the columns are not identical, the theoretical calculations will not be correct, and it will become difficult to design an efficient and robust continuous chromatography process. However, the loading/packing of a column with chromatography media composition, is very complex in order to obtain repeatable results. Even small differences in the number of plates or other packing properties can have a huge effect on the end result.
- A problem with conventional solutions is that performing continuous chromatography is a cumbersome, complex and time consuming operation. Often the process must be interrupted to perform reconnection of fluid couplings/tubes, to perform packing of columns or to load a pre packed column, to perform cleaning operations etc.
- Thus, there is a need for an improved chromatography apparatus for performing continuous chromatography.
- Furthermore, even though chromatography apparatus which performs continuous chromatography of the type mentioned above are known, for example those sold under the tradename of AKTApilot by GE Healthcare, and these known instruments function well, providing a versatile instrument which can be re-plumbed for different functionality if needed, these instruments lack some functionality, such that there are only certain procedures in which they can be employed, and an extension of that versatility would be commercially attractive, but is not easy to achieve with the competing restraints of instrument size, maintaining versatility, practical aspects such as ease of cleaning internally and externally, and cost.
- Embodiments of the present invention address the problems mentioned above. Thereby, an object of the present invention is to provide a chromatography instrument with one or more of: an increased functionality, for example able to operate in conventional batch chromatography as well as continuous chromatography; is useable across a wider range of applications; does not have a substantially increased overall size or manufacturing cost; and is simple to operate.
- Good Manufacturing Practice (GMP) sets out guidelines for bioprocessing procedures, which if followed require cleanliness standards. Advantageously, the standards are easier to achieve with the proposed apparatus, for example where fluid paths in the instrument have, in one configuration at least, a continuous flow path with no substantive stagnant portions, thereby providing complete cleaning without the need to break down the fluid conduits. Embodiments of the proposed instrument provide a sanitary small-scale chromatography instrument suitable for both GMP and non-GMP work. Functionally wide flow and pressure ranges of the instrument makes it fit for both production of technical batches and scale-up studies as well as small-scale production of GMP-grade material. The high accuracy and flow range of the pumps enables precise gradient formation, covering a large range of chromatography column sizes and more repeatable results.
- In embodiments, a modular construction provides increased functionality, for different uses. Interactive control software allows changes to be made in real-time and unexpected deviations to be quickly identified. The small, bench-top size frees up lab space. The instrument allows in-situ column packaging, i.e. the ability to compress chromatography media in the column, or each column where two or more columns are used, whilst being connected to the instrument, and without having to then disconnect any fluid conduits prior to performing chromatographic procedures.
- According to an aspect of the invention, there is provided a chromatography instrument as claimed herein having features set out the in independent apparatus claims, are further preferred features set out in the dependent apparatus claims.
- According to another aspect of the invention, there is provided a method for operating a chromatography instrument as claimed herein having features set out the in independent method claims, are further preferred features set out in the dependent method claims.
- The invention extends to any features described herein. Where embodiments show and/or describe features in combination herein, a claim which includes just one or a subset of said combined features is expressly considered to fall within the ambit of the invention disclosed herein.
- More advantages and benefits of the present invention will become readily apparent to the person skilled in the art in view of the detailed description below.
- The invention will now be described in more detail with reference to the appended drawings, wherein:
-
FIG. 1 shows a pictorial view of a new chromatography apparatus; -
FIG. 2 shows schematic representation of the apparatus shown inFIG. 1 ; and -
FIGS. 3, 4 and 5 show configurations of the apparatus shown in the previous Figures. -
FIG. 1 sows achromatography apparatus 100 according to an aspect of the invention. The apparatus includes, but it not limited to,individual modules 1 to 25 as listed below, at least some of which are demountable from an aperturedfront panel 120 of ahousing 110 of theapparatus 100 and mounted thereon in one generally vertical plane, such that the liquid connections required between modules can be made only at thefront face 120. In practice the demountable modules have no more than two standard sizes which can, if needed, be repositioned on thepanel 120 to suit a different procedure. Each module has a serial bus communication connection and power connection so that its physical position is immaterial to a controller for example located in thehousing 110, or located remotely. Thereby, the modules can be regarded as modular and thereby repositionable and/or interchangeable. - The chromatography apparatus shown in
FIG. 1 has the following module modules: -
- 1 Control panel
- 2 pH monitor
- 3 Outlet valve 1-3,
port 1 can be used for waste - 4 Outlet valve 4-6
- 5 Conductivity monitor
- 6 Outlet valve 7-9
- 7 Pre-column Conductivity monitor
- 8 Column valve, including pre- and post-column pressure sensor
- 9 Bottles for pump rinsing solution
- 10 Inlet valve A1-A3
- 11 Inlet valve A4-A6
- 12 Inlet valve B1-B3
- 13 Inlet valve B4-B6
- 14 Fixed rubber feet
- 15 Adjustable feet
- 16 System pump A
- 17 System pump B
- 18 Flow restrictor, including system pressure monitor
- 19 Mixer module
- 20 Mixer valve
- 21 Air trap valve, including air sensor
- 22 Air trap
- 23 ON/OFF button
- 24 Holder for in-line filter (typical filterapsule shown)
- 25 UV monitor
- Modules can be omitted or repositioned as explained above. It will be apparent that some modules can be replaced with other modules or the space left by an omitted module can be filled with a blanking plate (see e.g. 26
FIG. 4 ). More than one of the same numbered modules can be used where necessary. - Fluid interconnections between the fluid manipulating modules of the apparatus i.e. all the modules listed above except
modules FIG. 1 , are made via fluid conduits in this case in the form of flexible plastics tubing, which can be readily coupled and uncoupled to corresponding ports of the fluid manipulating modules, in any desired configuration, for example using a coupling as disclosed in a related application GB1710279 filed 28 Jun. 2017 incorporated herein by reference. -
FIG. 2 shows one possible liquid interconnection configuration between the main modular modules of the chromatography apparatus, connected in this case to twochromatography columns lines 80. - At the heart of the
apparatus 100 is thecolumn valve 8, which in this case has a construction as disclosed in our co-pending patent application filed on the same day as this application, and having the title ‘VALVE UNIT FOR A CHROMATOGRAPHY APPARATUS’ and is incorporated herein by reference. Thevalue unit 8 provides multiple switching of flow for allowing flow in one or both 20columns 200/300 in either direction (up or down in the drawing). The user can select upflow or downflow, or select to bypass one or both columns. The flow can be directed to waste or to the next component in the flow path. The columns can also be connected in series. The Column valve includes pre-column and post-column pressure sensors. In addition the valve has a port 150 which can be used to change the volume ofhydraulic cylinders 210 are 310 which are part of thecolumns value unit 8 measuring back-pressure resulting from pressure within the columns and in accordance with to known protocols, for example as described inWO200704549 1, which disclosure is incorporated herein by reference. - The remaining
system 100 comprises: -
- Inlet valve groups A and B, 10, 11, 12 and 13, suitable for providing selectable liquids including sample containing liquids buffer solutions, and cleansing fluids;
- The inlet valves supply two system pumps, here each having a pair of pistons and associated one-5 way valves, providing a variable flow rate of between 0-600 nil per minute each (1200 ml/min max), with a high volume and resolution of flow, enabling accurate flow rates to be maintained. Such accuracy enables good repeatability of results for a wide range of column diameters;
- The pumps supply, in series a
flow restrictor 18, which includes a system pressure monitor, amixer valve 20, and amixer module 19, before pumped liquid is diverted to thecolumn valve unit 8; - Any entrained air can escape via an
air trap valve 21, and anair trap vent 22, which vent also has an air escape from thecolumns - Once liquids reach the column valve unit they can be routed in accordance with the arrangement described in said co-pending application with the title ‘VALVE UNIT FOR A CHROMATOGRAPHY APPARATUS’, and thereby numerous modes of chromatography can be performed, from simple batch work, where a straightforward chromatographic separation process is performed using just one column, to procedures which more closely copy larger scale commercial procedures where two or more columns can be employed, one being readied for use while the other is being used for separation. The valve port numbering used is the same as that used in said co-pending application and here has the same arrangement;
- Output from the chromatography column(s) is passed out through
port 120 to: aconductivity monitor 5, a UVlight absorption monitor 25, and apH monitor 2, and is thence directed into an appropriate storage vessel in dependence on the signals from the three monitors, and thereby, separated fractions are collected in anappropriate vessel 501. Column washings can be collected in awaste vessel 500; - The long chain dotted lines in
FIG. 2 represent asystem bus 500 which carries signals and power to and from the modules mentioned above, to and from acontroller 600. It will be appreciated that controlling and monitoring signals may be transmitted wirelessly according to known protocols, doing away with the need for a communication bus. Thechromatography system 100 includes also a display screen 30. Software running on the controller will display plural icons on the screen 30 and allow user manipulation of the icon on the screen to drag and drop the icons to form a series of icons representative of a user defined chromatography control method, for ease of use.
-
FIGS. 3, 4 and 5 show the system connected with tubing for various configurations, where only some of the modules referenced inFIG. 1 remain in place in these figures, and the apertures left by removed modules are blanked off with blankingplates 26, screwed into place over the aperture to prevent accidental liquid ingress into thehousing 110. - In
FIG. 3 asystem 100′ with a configuration of modules suitable for regulated environments where systems are custom-built in a factory. The system is delivered mounted, calibrated, and performance tested and suitable for work in GMP environments.FIG. 4 shows one system with some modules removed, andFIG. 5 shows asystem 100′″ with more modules in place, similar toFIG. 1 , and showing typicaltubular interconnections 80. - In use, modules are easily removed or added to the system and installation finalized through a one-click activation in software which can recognize each module. The software can provide comprehensive and customizable operational control as well as pre-emptive maintenance. In addition to the modules described above, input-output communication modules can be used to interface with analog and/or digital external sensors or other equipment such as automatic fraction collecting devices. The wide flow rate and pressure ranges enables more than 40-fold scaling in the
range 25 to 250 mm internal diameter columns. This wide range makes the apparatus suitable to bridge the transition into GMP environments. - The packing (and re-packing) of chromatography columns, using the system described above is controllable fully by the
controller 600 initiated by thecontrol panel 1. Thecontroller 600 is able to drive the a display screen 30 (FIG. 2 ) to aid visualisation of the packing process and progress. The control software includes an accessible column packing record. Columns packing records can therefore be defined, created, and updated from the software for traceability and quality assurance purposes. In addition, the record can be used to monitor column performance and provide statistics for usage, separation performance, and packing intervals. - The display screen can provide a process visualization which quickly gives an operator an overview of the system's function, progress through operational steps and alarms, only providing the desired amount of information at each step. The active flow path is always displayed in the process visualisation to minimize user errors. Real time changes can be made by selecting the appropriate process on the visualization screen, e.g. selecting or dragging icons on the screen. Control, graphical interfaces are provided for specific sections, such as the
column valve 8. - Preprogrammed steps are employed but these can be modified and saved as user-defined steps for added customization.
- The system described and illustrated above is designed for sanitary environments. For example, the
system chassis 110 is flat or curved without joints, gaps or significant concavities, other than at the edges of the faces, which makes it easy to wipe down and reduces the chance of dust and liquid trapping. The pH monitor 2 has in-line calibration and thecolumn valve 8 provides in-process column packing, so a closed flow path through operations can be employed, meaning that no breaks in the fluid path need be made throughout one or more chromatography column packing/regeneration stages and throughout the separation operation, - The invention is not to be seen as limited by the embodiments described above, but can be varied within the scope of the appended claims as is readily apparent to the person skilled in the art.
Claims (17)
1. A chromatography system comprising:
a plurality of modules including at least one pump and a column valve unit connectable to plural chromatography columns; and
a controller, the controller being operable to control the or each pump and the column valve unit to perform different chromatographic processes, including chromatography employing just one column, as well as chromatography employing two or more columns by selective valve opening in said column valve unit, and wherein at least one of said plurality of modules is demountable from an apertured front panel of a housing of the chromatography system.
2. The chromatography system as claimed in claim 1 , wherein said at least one of said plurality of modules is mounted in one generally vertical plane, such that liquid connections required between respective modules can be made only at a front face of the chromatography system.
3. The chromatography system as claimed in claim 1 , wherein said selective valve opening allows a reversal of flow directions in use in one or more of said plural chromatography columns.
4. The chromatography system as claimed in claim 1 , wherein the controller is operable to control said modules according to software, and further comprising a display screen, said software being suitable to display plural icons on said screen and allowing user manipulation of the icon on the screen to drag and drop the icons to form a series of icons representative of a user defined chromatography control method.
5. The chromatography system as claimed in claim 4 , wherein the user defined method includes a continuous chromatography process employing said two or more chromatography columns.
6. The chromatography system as claimed in claim 1 , wherein the controller is further operable to perform one or more of column packing, column packing testing, and cleaning of at least a part of the system.
7. The chromatography system as claimed in claim 1 , further comprising plural chromatography columns, each column including a chamber of changeable volume for housing chromatographic separation media and an adapter moveable to increase or decrease each said volume, and wherein the column valve unit is in fluid communication with each said adapter and is selectively operable to move independently or collectively each said adapter by means of fluid pressure changes to consequently change each said volume and in use to cause compression or relief from compression of media within each said column volume.
8. The chromatography system as claimed in claim 1 , wherein the plural modules are interchangeably mountable in apertures of one generally vertical face of the system housing, the modules being adapted for selective fluidic interconnection by tubing, and wherein at least one of said modules includes said column valve unit.
9. The chromatography system of claim 8 , wherein the modules are adapted for selective fluidic interconnection by tubing substantially at said one generally vertical face such that in use the modules and tubing occupy a generally vertically extending volume to minimize the footprint of the system.
10. The chromatography system as claimed in claim 8 , wherein, other than said one generally vertical face, the system housing has flat or curved faces without joints other than at the edges of the faces.
11. The chromatography system as claimed in claim 1 , wherein the modules comprise one or more of: a pH monitor; an outlet valve; a conductivity monitor; a column valve; an inlet valve; a system liquid pump; a flow restrictor; a mixer; an air trap; an air trap valve; and air trap vent and a UV monitor.
12. The chromatography system as claimed in claim 11 , wherein output from the chromatography column(s) is passed out through a port to a conductivity monitor, a UV light absorption monitor, and a pH monitor, and is thence directed into an appropriate storage vessel in dependence on the signals from the three monitors such that separated fractions are collected in an appropriate vessel.
13. The chromatography system as claimed in claim 12 , wherein output from column washings is collected in a waste vessel.
14. The chromatography system as claimed in claim 1 , wherein the system includes two liquid pumps, one of said pumps being selectively operable alone or both pumps being selectively operable together, thereby to provide a range of fluid flow which is suitable for more than one mode of chromatography process.
15. The chromatography system as claimed in claim 1 , further comprising a pre-column conductivity monitor and/or adjustable feet.
16. The chromatography system as claimed in claim 1 , wherein the column valve unit further comprises a port which can be used to change the volume of hydraulic cylinders so as to provide compression of the contents of column(s).
17. The chromatography system as claimed in claim 1 , wherein the chromatographic processes include a continuous chromatography process that includes control of at least one pump and where the column valve unit is switched to provide fluid flow from the at least one pump in both directions in each of the columns employed.
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US18/350,588 US20230349867A1 (en) | 2017-09-22 | 2023-07-11 | Chromatography Apparatus |
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GBGB1715403.0A GB201715403D0 (en) | 2017-09-22 | 2017-09-22 | Chromatography apparatus |
PCT/EP2018/075693 WO2019057936A2 (en) | 2017-09-22 | 2018-09-21 | Chromatography apparatus |
US202016647983A | 2020-03-17 | 2020-03-17 | |
US18/350,588 US20230349867A1 (en) | 2017-09-22 | 2023-07-11 | Chromatography Apparatus |
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PCT/EP2018/075693 Continuation WO2019057936A2 (en) | 2017-09-22 | 2018-09-21 | Chromatography apparatus |
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US3166929A (en) * | 1961-07-13 | 1965-01-26 | Technicon Chromatography Corp | Chromatography analysis apparatus with standardization provision |
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JPH07229885A (en) * | 1994-02-16 | 1995-08-29 | Takeda Chem Ind Ltd | Automatic separator of sample |
JP2000097924A (en) * | 1998-09-18 | 2000-04-07 | Shimadzu Corp | Analysis device |
JP4228502B2 (en) * | 2000-03-08 | 2009-02-25 | 株式会社島津製作所 | Liquid chromatograph and flow path switching valve |
KR101280731B1 (en) * | 2005-05-20 | 2013-07-01 | 지이 헬스케어 바이오-사이언시스 에이비 | Device and method for automated packing of chromatography columns |
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AU2008223363B2 (en) | 2007-03-06 | 2012-09-27 | Global Life Sciences Solutions Usa Llc | System and method for the automation of column and media packing |
WO2008156484A1 (en) * | 2007-06-19 | 2008-12-24 | Neal Arnold | Apparatus, system, and method for low cost high resolution chemical detection |
USRE47124E1 (en) * | 2009-06-09 | 2018-11-13 | Ge Healthcare Bio-Science Ab | Automated fluid handling system |
US8656955B2 (en) | 2010-05-20 | 2014-02-25 | Bio-Rad Laboratories, Inc. | Rotary column selector valve |
KR101120943B1 (en) * | 2010-06-25 | 2012-03-05 | 고려대학교 산학협력단 | Multi-functional selection valve, multi-functional and fully automated liquid chromatography device with the same, and sample analysis method using the same |
US20130206653A1 (en) * | 2010-10-29 | 2013-08-15 | John E. Brann | Modular Multiple-Column Chromatography Cartridge |
US9304518B2 (en) * | 2011-08-24 | 2016-04-05 | Bio-Rad Laboratories, Inc. | Modular automated chromatography system |
CN103907020B (en) * | 2011-10-28 | 2017-10-13 | 萨默费尼根有限公司 | Method and system for liquid chromatogram fluid monitoring |
WO2015011522A1 (en) | 2013-07-23 | 2015-01-29 | Tubitak Ume | Multi-task sample preparation system with reconfigurable modules for on-line dilution, enzymatic digestion and fractionation |
EP3105579A4 (en) | 2014-02-11 | 2017-08-09 | Büchi Labortechnik AG | Multiple column chromatographic system and methods of use |
EP3105580B1 (en) | 2014-02-14 | 2023-10-04 | Cytiva Sweden AB | Automated multi-step purification system |
WO2016061558A1 (en) * | 2014-10-16 | 2016-04-21 | Practichem Llc | Web-based interactive process facilities and systems management |
US11938418B2 (en) | 2014-12-18 | 2024-03-26 | Cytiva Sweden Ab | Automated chromatography column switching control based on pressure detection |
JP6458504B2 (en) * | 2015-01-14 | 2019-01-30 | 株式会社島津製作所 | Supercritical fluid-liquid chromatograph and its analysis method |
CA3001916A1 (en) * | 2015-10-16 | 2017-04-20 | Lonza Ltd. | System and method for regulating cell culture based production of biologics |
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CN111094972A (en) | 2020-05-01 |
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