CN117322347A

CN117322347A - Plant tissue culture device capable of automatically cleaning and adjusting temperature and control method

Info

Publication number: CN117322347A
Application number: CN202311566531.2A
Authority: CN
Inventors: 高秀君; 刘金坤; 刘冰; 张洪喜; 张明臣
Original assignee: Shandong Anran Nanometre Ind Development Co ltd
Current assignee: Shandong Anran Nanometre Ind Development Co ltd
Priority date: 2023-11-22
Filing date: 2023-11-22
Publication date: 2024-01-02

Abstract

The invention discloses a plant tissue culture device capable of automatically cleaning and regulating temperature and a control method thereof, comprising the following steps: the culture tank comprises a jacket, an air inlet device and a shearing device; the temperature adjusting system comprises a supply unit, a conveying unit and a temperature adjusting water tank communicated with the supply unit, wherein the supply unit carries various temperature adjusting mediums with different temperatures, and the temperature adjusting mediums are led in and out of the jacket and/or the temperature adjusting water tank through the conveying unit; the control system controls the temperature adjusting system to select a temperature adjusting medium which is introduced into the jacket to adjust the temperature of the culture device, controls the cleaning system to feed water into the culture tank for cleaning water through a water inlet of the culture tank, and introduces steam into the culture tank for cleaning and steam sterilization through the air inlet device. The plant tissue culture device capable of automatically cleaning and regulating temperature can clean and steam sterilize a culture tank without dead angles before culture, and can also realize the regulation of culture temperature of the tissue culture device.

Description

Plant tissue culture device capable of automatically cleaning and adjusting temperature and control method

Technical Field

The invention belongs to the field of tissue and cell culture devices, and particularly relates to a plant tissue culture device capable of automatically cleaning and regulating temperature and a control method.

Background

The fermenter refers to an industrial device for microbial fermentation, has strict and reasonable structure, can resist steam sterilization, has certain operation elasticity, reduces internal accessories as much as possible (avoids dead angles), has strong material and energy transfer performance, can be adjusted to be convenient for cleaning and reducing pollution, and is suitable for production of various products and energy consumption reduction.

The proper temperature culture in the fermentation culture of biological cells is the most critical step in cell culture, and the effect of temperature control has direct influence on the culture and growth of cells in a fermentation tank. The existing biological fermentation tank is provided with a cooling system device, mainly comprises that industrial cold water is connected to an inlet of a cooling pipe of the fermentation tank, water flows into a cooling coil pipe in an inner cavity of the fermentation tank to be cooled circularly, and heat of cell solution in the inner cavity of the fermentation tank is taken away, so that the temperature of the cell solution is regulated to the temperature of process culture.

In the existing plant tissue culture process, before the culture solution and plant tissue seeds are added into the culture tank, the culture tank is ensured to be clean and sterile, so that the culture tank is cleaned and disinfected. However, due to the fact that the culture solution has certain viscosity, the problem that a certain position cannot be cleaned in the cleaning process possibly exists, pollution is caused to the newly added culture solution, and the culture of plant tissues is affected.

The present invention has been made in view of this.

Disclosure of Invention

The invention aims to solve the technical problems of overcoming the defects of the prior art, and provides a plant tissue culture device capable of automatically cleaning and regulating temperature and a control method thereof, which can clean a culture tank without dead angles before culturing, and can also introduce media with different temperatures to regulate the temperature in the process of culturing plant tissues in the process of culturing the plant tissues so as to ensure that the temperature in the plant tissue culture tank is suitable for the growth of plant tissues.

In order to solve the technical problems, the invention adopts the basic conception of the technical scheme that:

in a first aspect of the present invention, there is provided a plant tissue culture apparatus for automatic cleaning and tempering comprising:

the culture tank comprises a jacket sleeved outside the tank body, an air inlet device and a shearing device which are arranged at the bottom of the tank body;

the temperature adjusting system comprises a supply unit, a conveying unit and a temperature adjusting water tank communicated with the supply unit, wherein the supply unit carries various temperature adjusting mediums with different temperatures, and the temperature adjusting mediums are led in and out of the jacket and/or the temperature adjusting water tank through the conveying unit so as to realize the adjustment of the culture temperature of the plant tissue culture device;

The cleaning system comprises a water inlet arranged on the culture tank and an air inlet branch pipe for introducing steam into the air inlet device;

the control system is in communication connection with the culture tank, the temperature adjusting system and the cleaning system;

the control system controls the temperature adjusting system to select a temperature adjusting medium entering the jacket to adjust the temperature of the culture device, controls the cleaning system to feed water into the culture tank through the water inlet of the culture tank to clean water, and feeds steam into the culture tank through the air inlet device to clean and steam sterilize the culture tank.

Further, the temperature-regulating water tank is provided with a water containing cavity, and the water containing cavity and the jacket are connected with the supply unit through the conveying unit to form a circulation loop; the conveying unit comprises a water inlet pipe and a water outlet pipe which are communicated with the supply unit and the water containing cavity; and a water pump is arranged in the water inlet pipe in series and used for conveying the water in the water containing cavity to the jacket.

Further, the temperature adjustment system further includes: the main control unit is used for controlling the proportion of the chilled water and the high-temperature steam which are conveyed to the heat exchange structure of the temperature-adjusting water tank by the liquid inlet pipe; the heat exchange structure is arranged close to the water containing cavity.

Further, the supply unit includes a plurality of medium supply lines including a plurality of medium supply lines arranged in parallel

A hot water supply main pipe for supplying a medium of a first temperature; a high-temperature steam supply main pipe for supplying a medium of a second temperature; a chilled water supply main pipe for supplying a medium of a third temperature; a cooling water supply pipe for supplying a medium of a fourth temperature; the third temperature is less than the fourth temperature is less than the first temperature and less than the second temperature;

the conveying unit comprises a plurality of medium input branch pipelines and a first main pipeline which are connected in parallel, and the medium conveying branch pipelines are alternatively communicated with the first main pipeline;

the medium input branch pipelines are in one-to-one correspondence with the medium supply pipelines, and alternatively, the medium with preset temperature is input into the jacket.

Advantageously, the medium supply pipeline can provide fluid mediums with different temperatures, and then the fluid mediums are input into the jacket through medium input branch pipelines in one-to-one correspondence, so that the temperature regulation function is realized, and the medium selection is increased.

Further, the high-temperature steam supply pipeline is provided with a plurality of branches, one branch is connected with an air inlet branch pipe, the air inlet branch pipe is connected with an air inlet device, steam is introduced into the culture tank through the air inlet device, and the culture device is cleaned and steam disinfected.

Further, the supply unit further includes: a plurality of medium recovery pipelines; the conveying unit further includes: the medium in the jacket is led into the medium recovery pipeline through the medium output branch pipeline;

the plurality of output branch pipelines are alternatively communicated with the second main pipeline to lead out the medium in the jacket; each of the output branch lines is controllably in communication with the second main line.

Advantageously, the plurality of output branch pipelines are communicated with the jacket through one main pipeline, so that the arrangement of outlets on the jacket is reduced, and different mediums are ensured to enter corresponding medium recovery pipelines through different branch pipelines.

Further, on-off valves are arranged on the input branch pipelines and the output branch pipelines, and the input branch pipelines communicated with the first main pipeline and/or the output branch pipelines communicated with the second main pipeline are controlled by the on-off of the on-off valves.

Advantageously, the provision of the on-off valve allows the input branch and the output branch of the connection to be selected by the on-off of the on-off valve and ensures that the selected input branch corresponds to the output branch.

In a second aspect of the present invention, a control method of a plant tissue culture apparatus for automatic cleaning and temperature adjustment is provided, and the method is applied to the plant tissue culture apparatus for automatic cleaning and temperature adjustment, and includes:

S100, before the culture solution is added into the culture tank, controlling a cleaning system to feed water into the culture tank through a water inlet on the culture tank so as to clean the culture device;

s200, after the cleaning is finished, discharging used water;

s300, repeating the steps S100-S200 at least three times, and adding the culture solution and the plant tissue seeds into the culture tank for culture.

Optionally, in step S100, at least: the control system controls the air inlet device arranged at the bottom of the culture tank to enter air, and simultaneously controls the shearing device to work so as to finish the simultaneous cleaning of the shearing device.

Optionally, in step S300, when the last cleaning is performed, the water inlet on the culture tank is controlled to feed water into the culture tank, the valve body of the air inlet branch pipe is controlled to be opened, steam is introduced into the culture tank through the air inlet device, cleaning and steam sterilization are performed, and meanwhile, the shearing device is controlled to work, so that the simultaneous cleaning of the shearing device is completed.

Optionally, in step S300, after the last cleaning is completed, the method includes:

s301, adding culture solution into a culture tank;

s302, introducing steam into the culture tank through the air inlet device to sterilize the steam;

s303, controlling a temperature adjusting system to adjust the temperature of the culture device to be reduced to a proper culture temperature;

S304, adding plant tissue seeds for culturing, and controlling a temperature adjusting system to adjust the temperature in the culturing process.

By adopting the technical scheme, compared with the prior art, the invention has the following beneficial effects.

1. According to the invention, the mediums with different temperatures are introduced into the jacket sleeved outside the tank body of the culture tank, a layer of spacer is arranged between the medium and the tank body, so that the medium can be prevented from polluting the culture solution in the tank body, and the medium with different temperatures can be introduced to keep the temperature of the plant tissue culture tank suitable for the growth of plant tissues.

2. The temperature adjusting system is additionally provided with the temperature adjusting water tank, and the temperature adjusting water tank and the jacket are connected to the supply unit, so that the temperature adjusting water tank and the jacket form a loop connected in parallel with the supply unit, the temperature adjusting water tank and the jacket can circulate heat exchange medium with the supply unit at the same time, and the temperature adjusting water tank and the jacket can be connected in series by the supply unit to form a temperature adjusting loop, so that the temperature adjusting water tank and the jacket can circulate directly, the temperature adjusting loop can be used for independently adjusting the temperature of the plant tissue culture tank when a supply source of the temperature adjusting system is damaged and maintained, and the fault tolerance of the temperature adjusting system is improved.

3. According to the invention, the steam inlet branch pipeline is arranged on the jacket, so that hot steam can be introduced into the jacket of the tank body before culturing, the tank body can achieve the effect of rapid temperature rise, and the time for preheating the tank body is reduced.

4. According to the invention, water is fed into the culture tank through the water inlet of the culture tank, and steam is introduced through the air inlet device, so that the culture tank before culture can be automatically cleaned and disinfected, and the smooth secondary culture is ensured.

The following describes the embodiments of the present invention in further detail with reference to the accompanying drawings.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention. It is evident that the drawings in the following description are only examples, from which other drawings can be obtained by a person skilled in the art without the inventive effort. In the drawings:

FIG. 1 is a schematic diagram of a tempering system for a plant tissue culture tank according to the present invention;

FIG. 2 is a schematic diagram of a piping structure of a continuous culture apparatus according to the present invention;

FIG. 3 is a schematic diagram showing a piping structure of another continuous culture apparatus according to the present invention;

FIG. 4 is a schematic illustration of an air inlet device of the present invention connected to a plant tissue culture tank;

FIG. 5 is a schematic view of an air intake apparatus according to the present invention;

FIG. 6 is a schematic cross-sectional view of an air intake device according to the present invention;

FIG. 7 is a schematic view showing a structure in which a shearing device is provided on the bottom wall of a culture tank in the present invention;

FIG. 8 is a schematic view of the structure of the shearing device in the present invention;

FIG. 9 is a schematic view of the structure of a shearing section of the shearing device in the present invention;

FIG. 10 is a top view showing the distribution structure of the air inlet device and the shearing device on the bottom wall of the seed tank;

FIG. 11 is a plan view showing the distribution structure of the air inlet device and the shearing device on the bottom wall of the culture tank according to the present invention.

In the figure: 1. a tank body; 11. a bottom wall; 2. a jacket; 21. a first main line; 22. a second main line; 5. an on-off valve; 70. an air intake device; 71. an air inlet part; 72. an aeration section; 73. a conduit; 731. an air inlet section; 732. an air outlet section; 733. a transition section; 74. a seat plate; 75. quick release joint; 451. sealing grooves; 51. an interface; 52. stacking the pressing piece; 521. a through hole; 53. a hub kit; 531. a support ring; 532. a groove; 533. a connection hole; 54. a seal ring; 8. an air inlet branch pipe; 9. a water inlet; e200, a hot water supply main pipe; e100, a hot water recovery main pipe; b100, a steam supply main pipe; c200, a chilled water supply main pipe; c100, a chilled water recovery main pipe; d200, a cooling water supply main pipe; d100, a cooling water recovery main pipe; c20, chilled water supply branch pipe; d20, cooling water supply branch pipes; e20, hot water supply branch pipes; c10, chilled water recycling branch pipes; d10, cooling water recycling branch pipes; e10, hot water recovery branch pipes; a1, a supply unit; a2, a conveying unit; a21, a water inlet pipe; a22, a water outlet pipe; a24, a liquid inlet pipe; a25, a liquid outlet pipe; a3, a temperature-regulating water tank; a32, a heat exchange structure; a301, a water containing cavity; 3. a shearing device; 90. a tank cavity; 31. a spacer bush; 311. a convex column; 312. a flange edge; 32. a shearing part; 321. a pin shaft; 322. a blade set; 323. a first blade; 324. a second blade; 325. a rotating end; 326. blade ends; 327. closing the side edges; 328. unfolding the side edges; 33. a transmission part; 331. a discharging section; 332. a drive section; 333. a discharge port; 34. a power section; 35. a guide cover; 351. an inlet; 352. a mounting port; 353. and an outlet.

It should be noted that these drawings and the written description are not intended to limit the scope of the inventive concept in any way, but to illustrate the inventive concept to those skilled in the art by referring to the specific embodiments.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions in the embodiments will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present invention, and the following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.

In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.

The invention provides a plant culture device capable of automatically cleaning and adjusting temperature, which is provided with a pipeline structure as shown in figures 1, 2 and 3. Wherein, the plant tissue culture device at least comprises two tanks 1 for cultivating plant isolated tissues or cells. In particular, the tanks 1 are connected in parallel, and each tank 1 is supplied with air, food, heat, cleaning, disinfection and other substances independently by a supply source through a pipeline.

Specifically, the pipeline structure includes: main pipeline and branch pipeline. The main pipeline and the continuous culture device are arranged separately.

The main pipelines are connected with a plurality of devices in parallel, and meanwhile, the main pipelines extend along a straight line and are parallel to each other. Each tank 1 is provided with a branch line for exchanging and circulating substances with the main line. The branch pipeline is connected between the main pipeline and the continuous culture device and communicates the main pipeline with the continuous culture device.

In this embodiment, the pipeline structure that will be connected to culture apparatus has carried out the optimal arrangement according to the size that intercepts, wherein, main pipeline is intercepted greatly for concentrate the material of supplying with, and the branch pipeline is intercepted little, is used for leading-in culture apparatus with the material of main pipeline, mutual parallel arrangement between the different main pipelines, even so that can connect a plurality of branch pipelines in the extending direction of every main pipeline, still can discern the pipeline fast during the maintenance of being convenient for to improve pipeline structure's regularity, reduced the invalid occupation of pipeline to the space.

Specifically, the pipeline structures are respectively provided with main pipelines for supplying and recovering fluid media with different temperatures, and the main pipelines are connected into a complete circulation loop through branch pipelines corresponding to the main pipelines, so that the pipeline structures can exchange heat with the culture device, and stable growth conditions are provided for proliferation of plant in-vitro tissues such as adventitious roots. And the parallel arrangement among different main pipelines ensures that the pipeline structure is regular and the maintenance is convenient.

In an embodiment of the present invention, a plant tissue culture apparatus for automatic cleaning and tempering includes:

the culture tank comprises a jacket 2 sleeved outside the tank body 1, an air inlet device 70 and a shearing device 3 which are arranged at the bottom of the tank body 1;

the temperature adjusting system comprises a supply unit A1, a conveying unit A2 and a temperature adjusting water tank A3 communicated with the supply unit A1, wherein the supply unit A1 carries various temperature adjusting mediums with different temperatures, and the temperature adjusting mediums are led in and out of the jacket 2 and/or the temperature adjusting water tank A3 through the conveying unit A2 so as to realize the adjustment of the culture temperature of the plant tissue culture device;

the cleaning system comprises a water inlet 9 arranged on the culture tank and an air inlet branch pipe 8 for introducing steam into the air inlet device 70;

The control system controls the temperature adjusting system to select a temperature adjusting medium which is introduced into the jacket 2 to adjust the temperature of the culture device, controls the cleaning system to feed water into the culture tank for cleaning water through the water inlet 9 of the culture tank, and introduces steam into the culture tank through the air inlet device 70 for cleaning and steam sterilization.

The steam is introduced into the culture tank through the air inlet device 70, or the steam is introduced into the culture tank before the culture solution is put into the culture tank, so that the culture tank is cleaned and steam sterilized; after the culture medium is placed in the culture tank, the air inlet device 70 may be used to introduce steam into the culture medium to sterilize the culture medium with steam.

In this embodiment, the temperature-adjusting water tank A3 is added in the temperature-adjusting system, and the temperature-adjusting water tank A3 and the jacket 2 are both connected to the supply unit A1, so that the temperature-adjusting water tank A3 and the jacket 2 form a loop parallel to the supply unit A1, the temperature-adjusting water tank A3 and the jacket 2 can circulate heat exchange medium with the supply unit A1 at the same time, and the temperature-adjusting water tank A3 and the jacket 2 can be connected in series by the supply unit A1 to form a temperature-adjusting loop, so that the temperature-adjusting water tank A3 circulates with the jacket 2 directly, and the temperature-adjusting loop can be used for independently adjusting the temperature of the plant tissue culture tank when the supply source of the temperature-adjusting system is damaged and maintained, thereby improving the fault tolerance of the temperature-adjusting system.

Further, the temperature-regulating water tank A3 is provided with a water containing cavity A301, and the water containing cavity A301 and the jacket 2 are connected with the supply unit A1 through the conveying unit A2 to form a circulation loop; the conveying unit A2 comprises a water inlet pipe A21 and a water outlet pipe A22 which are communicated with the supply unit A1 and the water containing cavity A301; the water inlet pipe A21 is internally and serially provided with a water pump for conveying the water in the water containing cavity A301 to the jacket 2.

Further, the temperature adjustment system further includes: the heat exchange structure A32 and the total control unit is electrically connected with the pneumatic valves connected in series in each branch of the liquid inlet pipe A24, and the total control unit is used for controlling the proportion of the liquid inlet pipe A24 for conveying chilled water and high-temperature steam to the heat exchange structure A32 of the temperature regulating water tank A3; the heat exchange structure A32 is arranged close to the water containing cavity A301.

The temperature regulating system is additionally provided with a temperature regulating water tank A3 and a heat exchange structure A32.

Specifically, the temperature-adjusting water tank A3 is provided with a water-containing chamber a301 for containing and heating the liquid that can be fed into the jacket 2. In addition, the heat exchange structure A32 is arranged close to the water containing cavity A301 and can exchange heat with the liquid in the water containing cavity A301, so that a circulation loop capable of supplying heat to the jacket 2 is formed between the temperature-adjusting water tank A3 and the jacket 2.

Wherein the water containing cavity A301 and the jacket 2 are connected with the supply unit A1 through the delivery unit A2 to form a circulation loop. The conveying unit A2 comprises a water inlet pipe A21 and a water outlet pipe A22 which are communicated with the supply unit A1 and the water containing cavity A301, and the temperature of the plant tissue culture tank is maintained by the temperature regulating water tank A3.

Further, the supply unit A1 includes a plurality of medium supply lines including a hot water supply main E200 provided in parallel for supplying a medium of a first temperature; a high-temperature steam supply main pipe B100 for supplying a medium of a second temperature; a chilled water supply main pipe C100 for supplying a medium of a third temperature; a cooling water supply pipe D100 for supplying a medium of a fourth temperature;

the third temperature is less than the fourth temperature is less than the first temperature and less than the second temperature;

the conveying unit A2 comprises a plurality of medium input branch pipelines and a first main pipeline 21 which are connected in parallel, wherein the medium input branch pipelines are alternatively communicated with the first main pipeline 21;

the medium input branch pipelines are in one-to-one correspondence with the medium supply pipelines, and alternatively input the medium with preset temperature into the jacket 2.

In particular, the medium supply line comprises a plurality of main lines arranged in parallel, and each main line is used for introducing different substances into the plant tissue culture tank or the jacket 2, respectively.

Specifically, the medium input branch pipeline is communicated with the medium supply pipeline, fluid mediums with different temperatures are carried in the medium supply pipeline, a plurality of supply main pipes corresponding to the mediums with different temperatures are arranged, when the mediums need to be introduced into the jacket 2, the mediums are led out from the corresponding supply main pipes in the medium supply pipeline, enter the medium input branch pipeline, and then are led into the jacket 2 by the medium input branch pipeline.

Further, the medium supply pipeline high temperature steam supply pipeline B100 is provided with a plurality of branches, one of which is communicated with the air inlet branch pipe 8, the air inlet branch pipe 8 is connected with the air inlet device 70, and steam is introduced into the culture device through the air inlet device 70 to clean and steam sterilize the culture device.

As shown in fig. 1, 2 and 3, in the present embodiment, the plurality of medium supply lines include: a hot water supply main pipe E200 for supplying hot water; a high-temperature steam supply main pipe B100 for supplying high-temperature steam; a chilled water supply main pipe C100 for supplying chilled water; and a cooling water supply pipe D100 for supplying cooling water.

In this embodiment, the input branch line includes: the hot water supply branch pipe E20 is communicated with the hot water supply main pipe E200, and the high-temperature steam supply branch pipe is communicated with the high-temperature steam supply pipe B100, and is used for introducing hot water/high-temperature steam into the first main pipeline 21 and then introducing the hot water/high-temperature steam into the jacket 2 to perform temperature rise adjustment on the culture temperature of the culture device.

In this embodiment, the input branch line includes: the chilled water supply branch pipe C20 is connected to the chilled water supply main pipe C100, and the cooling water supply branch pipe D20 is connected to the cooling water supply main pipe D200, and is used to introduce chilled water/cooling water into the first main pipe 21, and then to introduce the chilled water/cooling water into the jacket 2, thereby reducing and adjusting the culture temperature of the culture apparatus.

The control system controls the temperature adjusting system to adjust the flow ratio of the chilled water and the high-temperature steam to the temperature adjusting water tank A3 and the heat exchange structure A32. Chilled water and high-temperature steam enter the temperature-adjusting water tank A3 or the heat exchange structure A32 and can exchange heat with hot water in the water containing cavity A301, so that the hot water is ensured to meet the requirements of the plant tissue culture tank when being conveyed into the jacket 2. Even when the supply unit A1 cannot directly supply hot water to the jacket 2, the hot water can be supplied to the jacket 2 by the temperature-adjusting water tank A3, and the operation of the plant tissue culture tank can be maintained.

In another embodiment, an electric heating structure may be disposed in the temperature-adjusting water tank A3 to directly heat the liquid in the water containing cavity a 301. Thus, when the hot water cannot be directly supplied from the hot water supply main E200 to the jacket 2, the hot water supply main E200 and the hot water recovery main E100 can be controlled to directly communicate with the jacket 2 and the temperature-adjusting tank A3. In this way, a single circulation loop is formed between the temperature-regulating water tank A3 and the jacket 2, and the temperature of the hot water can be directly conveyed to the jacket 2 by utilizing the temperature-regulating water tank A3, so that the requirement of the culture tank on the temperature of the hot water is ensured.

In particular, during normal operation of the supply unit A1, the jacket 2 may be fed with hot water directly supplied from the hot water supply main E200 or with hot water fed from the temperature-adjusting water tank A3. At this time, the temperature of the hot water in the water containing chamber a301 can be further raised and then transferred into the hot water supply main E200, so that the temperature compensation effect can be achieved on the hot water in the hot water supply main E200, and the temperature of the culture tank can be more finely adjusted and controlled.

In this embodiment, the temperature-adjusting water tank A3 and the plant tissue culture tank are connected in parallel to the supply unit A1, so that the supply unit A1 can simultaneously convey hot water to the temperature-adjusting water tank A3 and the jacket 2, and the temperature-adjusting water tank A3 can also convey hot water to the jacket 2, thereby adding a standby heat supply source for the temperature-adjusting system, and ensuring that the plant tissue culture tank normally maintains and adjusts the temperature of the nutrient solution in the plant tissue culture tank when the supply unit A1 is damaged or maintained.

Specifically, the temperature-regulating water tank A3 has a heat exchanging structure a32 disposed against the water containing chamber a 301. The conveying unit A2 comprises a liquid inlet pipe A24 and a liquid outlet pipe A25 which are communicated with the supply unit A1 and the heat exchange structure A32 and are used for respectively leading in and leading out liquids with different temperatures to the heat exchange structure A32.

In this embodiment, by arranging the heat exchange structure a32 in the temperature-adjusting water tank A3, the hot water in the temperature-adjusting water tank A3 is always kept at the set temperature when the supply unit A1 is operated, so that the temperature-adjusting water tank A3 can immediately convey the hot water to the jacket 2 when the supply unit A1 stops supplying or maintaining, the water of the temperature-adjusting water tank A3 does not need to wait for heating, and the standby heat exchange loop of the temperature-adjusting system can quickly take over the main heat exchange loop to maintain the normal temperature of the plant tissue culture tank.

An electric heating structure can be arranged in the temperature-regulating water tank A3 to directly heat the liquid in the water containing cavity A301. Thus, when the hot water cannot be directly supplied from the hot water supply main E200 to the jacket 2, the hot water supply main E200 and the hot water recovery main E100 can be controlled to directly communicate with the jacket 2 and the temperature-adjusting tank A3. In this way, a single circulation loop is formed between the temperature-regulating water tank A3 and the jacket 2, and the temperature of the hot water can be directly conveyed to the jacket 2 by utilizing the temperature-regulating water tank A3, so that the requirement of the culture tank on the temperature of the hot water is ensured. In particular, during normal operation of the supply unit A1, the jacket 2 may be fed with hot water directly supplied from the hot water supply main E200 or with hot water fed from the temperature-adjusting water tank A3. At this time, the temperature of the hot water in the water containing chamber a301 can be further raised and then transferred into the hot water supply main E200, so that the temperature compensation effect can be achieved on the hot water in the hot water supply main E200, and the temperature of the culture tank can be more finely adjusted and controlled.

In the embodiment of the invention, the bottom of the tank body 1 is provided with the air inlet device 70 and the shearing device 3, and high-temperature steam can be introduced into the culture device through the air inlet device 70 for cleaning and steam sterilization; the steam supply main pipe B100 is provided with a plurality of branch pipes, which are respectively communicated with the steam supply branch pipe, the air inlet device 70 and the sterilizing medium inlet arranged at the bottom of the on-off valve 5, so as to perform high-temperature sterilization. When the culture tank is cleaned and steam sterilized, the operation of the shearing device 3 is controlled, and the shearing device 3 is cleaned, so that the shearing device 3 is cleaned without dead angles.

As shown in fig. 10 and 11, in particular, the plant tissue culture apparatus includes a tank 1, an air inlet device 70 provided at the bottom of the tank 1, and a shearing device 3 provided above the air inlet device 70. Wherein, the tank 1 is used for cultivating isolated tissues or cells of the plant body. The air intake device 70 is used to introduce air/high temperature steam into the tank 1.

As shown in fig. 4, 5 and 6, air intake device 70 includes air intake portion 71, aeration portion 72, and duct 73 connecting air intake portion 71 and aeration portion 72. Wherein the conduit 73 is formed in a straight rod shape, and the air inlet portion 71 and the aeration portion 72 are connected to both ends of the conduit 73, respectively.

Specifically, the center of the aeration portion 72 is provided with a cavity. The cavity communicates with the conduit 73 and the walls of the cavity are provided with micropores that communicate from outside the aeration portion 72 into the cavity. A large number of micropores are densely and uniformly distributed throughout the cavity wall.

Preferably, aeration portion 72 is made of a titanium alloy material such that adventitious roots cannot adhere to the outer surface of aeration portion 72, thereby avoiding adventitious root accumulation spoilage.

In this embodiment, a large number of micropores are densely formed on the side wall of the aeration portion 72, and all the micropores are communicated with a cavity formed in the center of the aeration portion 72, so that gas entering the cavity of the aeration portion 72 through the gas guide pipe can be sprayed into the solution from all the micropores at the same time, thereby forming a large number of small bubbles in the solution at the same time, the small bubbles move outwards from the outer surface of the aeration portion 72 and float upwards, so that the small bubbles are prevented from being polymerized into large bubbles, the contact and dissolution rate of the gas and the solution are improved, and the circulating flow of the solution is promoted by the floating movement of a large number of small bubbles in the solution in a dispersing manner, and the dissolved oxygen effect is further improved.

Specifically, the aeration portion 72 provided with the air intake mechanism has a long cylindrical shape, and one end of the aeration portion 72 is connected to the conduit 73. The center axis of the aeration portion 72 is arranged to coincide with the center axis of the tip of the conduit 73 and to extend in a straight line in a direction away from the conduit 73. Meanwhile, the conduit 73 is long and straight, so that the aeration portion 72 and the conduit 73 are arranged to coincide with the central axis.

A seat plate 74 provided in the air inlet device 70 is located in the middle between the air inlet portion 71 and the aeration portion 72, and separates the air inlet portion 71 and the aeration portion 72.

Preferably, the seat plate 74 is provided in a circular flat plate shape so that the air inlet device 70 can be rotated around the duct 73 when installed.

In this embodiment, the seat plate 74 is disposed on the air inlet device 70, so that the air inlet device 70 and the plant tissue culture tank are more convenient to install and connect, and the air inlet device 70 can rotate around the conduit 73, thereby reducing the difficulty of assembly work.

More specifically, the setting conduit 73 includes a long straight intake section 731. Air inlet sections 731 extend at least a length from each side of seat 74 to facilitate better attachment of aeration section 72 and air inlet section 71 to conduit 73.

Aeration section 72 and air intake section 71 are respectively connected to ends of air intake section 731 overhanging the sides of seat plate 74, thereby separating aeration section 72 and air intake section 71 from both sides of seat plate 74.

In another embodiment of the present invention, to increase the location of aeration portion 72 within the plant tissue culture tank after it has been installed therein, conduit 73 is further provided with an air outlet section 732 disposed at an angle to air inlet section 731.

An outlet section 732 is connected at one end to the inlet section 731 and at the other end to the aeration section 72. In particular, the angle between the outlet section 732 and the inlet section 731 is set to be greater than 90 °.

In another embodiment, to further widen the range of positions of aeration sections 72 within the plant tissue culture tank in cooperation with seat plate 74, conduit 73 is further provided with a transition section 733 connected between the inlet section 731 and the outlet section 732.

Specifically, the transition 733 is disposed parallel to the seat plate 74. One end of the transition section 733 is connected to the inlet section 731 and the other end is connected to the outlet section 732. The air outlet section 732 is disposed obliquely to the transition section 733 and extends from the transition section 733 in a direction away from the seat plate 74.

In the present embodiment, a quick disconnect 75 is provided at the end of the conduit 73 in the air intake portion 71. The quick release connector 75 is conical in shape. The conical bottom surface of the quick release coupling 75 is superposed on the air inlet end surface of the duct 73. The inlet of the duct 73 is located in the center of the bottom surface. The tapered surface of the quick release connector 75 is directed toward the seat plate 74 and gradually contracted to the outer peripheral surface of the guide pipe 73.

In another embodiment of the present invention, as shown in fig. 4-6, a quick disconnect 75 having a sealed air inlet 70 is described.

The conical bottom surface of the quick disconnect 75 is provided with a seal groove 451. The seal groove 451 is recessed from the bottom surface toward the inside of the quick release coupling 75 and forms a recess in the bottom surface. In particular, the seal groove 451 surrounds the outer periphery of the air inlet of the duct 73.

The invention also provides a plant tissue culture tank with any one of the air inlet devices 70. The bottom of the plant tissue culture tank is arranged into an inverted cone shape. The conical side wall is provided with a connection 51 for connecting to an air inlet device 70. The air inlet device 70 is connected with the interface 51, the aeration part 72 of the air inlet device is positioned in the cavity of the plant tissue culture tank, and the air inlet part 71 is suspended outside the plant tissue culture tank.

In this embodiment, by providing the air inlet device 70, when adventitious roots are cultivated, a large number of small bubbles can be formed in the solution by air, the small bubbles move outwards and upwards from the aeration portion 72 to promote the circulation flow of the nutrient solution, and the large number of small bubbles are dispersed in the solution to further improve the contact and dissolution rate of the gas and the nutrient solution, provide stable conditions for the proliferation of the adventitious roots, and facilitate the improvement of the cultivation efficiency and quality of the adventitious roots.

In another embodiment of the present invention, as shown in FIG. 4, a mounting assembly of an air inlet device 70 capable of rotating at an arbitrary angle about its central axis when connected to a culture tank is introduced. The mounting assembly includes a stack 52 having an annular shape.

When the air inlet device 70 is fixedly installed, the stacking piece 52 and the interface 51 on the culture tank are connected by end surfaces and the seat plate 74 is clamped between the stacking piece 52 and the interface 51, so that the seat plate 74 can be rotated when the stacking piece 52 and the interface 51 are not tightly attached, and the position and the posture of the air inlet device 70 relative to the culture tank can be adjusted.

Specifically, the lamination 52 is provided as an annular structure having an annular hole in the center. The stacking member 52 is sleeved with the seat plate 74 and abuts with the edge of the annular ring thereof against the plate surface of the seat plate 74 facing the outside of the plant tissue culture tank for clamping the air inlet device 70 to the plant tissue culture tank.

In another embodiment, the mounting assembly further includes a hub sleeve 53 having a cylindrical outer peripheral surface. The hub sleeve 53 is nested in the interface 51 and connected to the interface 51 with its outer peripheral surface. In particular, the axis of the hub assembly 53 is perpendicular to the surface of the bottom wall 11 of the culture tank and projects in the axial direction of itself to the inside and outside of said bottom wall 11.

Meanwhile, an inner circumferential surface of the hub unit 53, which forms a center hole, is coupled to the seat plate 74.

Further, the end of the hub assembly 53 facing into the bottom wall 11 is provided with a support ring 531. The support ring 531 extends from the inner circumferential surface of the hub kit 53 in the radial direction toward the central axis of the hub kit 53, and forms a through hole 521 that can pass through the aeration portion 72.

The seat plate 74 is fitted to the inner peripheral surface of the hub unit 53 from the outside of the bottom wall 11, and the seat plate 74 abuts against the support ring 531.

In another embodiment, a more leak-tight mounting assembly is described. The mounting assembly includes a seal 54 for filling the gap between the contact surfaces.

Specifically, the seal ring 54 is made of rubber material, and has elasticity and good ductility. The seal ring 54 is provided between the seat plate 74 and the support ring 531, and is sandwiched between an end surface of the seat plate 74 facing the inside of the can and an end surface of the support ring 531.

In particular, when the seat plate 74 and the support ring 531 sandwich the seal ring 54, the thickness of the seal ring 54 is reduced, so that the projections and recesses on the end surface of the seat plate 74, which is close to the support ring 531, are filled with the seal ring 54, improving the sealability of the mounting assembly of the air inlet device 70.

Further, a support ring 531 is provided at the end of the hub kit 53 located within the bottom wall 11. The through hole 521 of the support ring 531 is arranged coincident with the central axis of the central hole of the hub unit 53. And, one end face of the support ring 531 is flush with the end face of the hub unit 53 and the other is located in the central bore of the hub unit 53.

In particular, the support ring 531 is provided with a recess 532 in the end surface of the hub sleeve 53 that is located in the central bore. The opening of the recess 532 is directed towards the outside of the bottom wall 11. The seal ring 54 is embedded in the groove 532 and protrudes from the end surface of the support ring 531. When the fixed air inlet device 70 is installed, the seat plate 74 presses the seal ring 54, so that the seal ring 54 deforms to fill the groove 532.

In this embodiment, the seat plate 74 of the mounting assembly is in a sleeved fit with the hub sleeve 53 in a gap, the seat plate 74 is sleeved in the central hole of the hub sleeve 53 to flexibly rotate, and the seat plate 74 is clamped and fixed in the hub sleeve 53 by pressing the lamination piece 52 from the other side of the seat plate 74.

Specifically, the end surface of the hub member 53 protruding outside the bottom wall 11 of the culture tank is provided with a connection hole 533. The connection hole 533 extends along the axial direction of the hub sleeve 53 for fixing the lamination member 52.

The lamination 52 is connected to the hub assembly 53 at an end face, and sandwiches the seat plate 74. And the lamination member 52 is provided with a through hole 521 corresponding to the connection hole 533 for passing through the fixing screw.

In another embodiment of the invention, as shown in FIGS. 7 to 9, the axial direction of the shearing device 3 forms an angle with the bottom wall 11 of the culture tank, preferably 90 degrees

The shearing device 3 comprises a spacer 31, a shearing part 32, a transmission part 33, a power part 34 and a guide cover 35.

The bottom wall 11 of the culture tank is provided with a circular mounting hole, and the outer circumferential surface of the spacer 31 is cylindrical and has the same diameter as the mounting hole. The spacer 31 is detachably fitted into the mounting hole in the bottom wall 11.

One side of the spacer 31 is connected with the shearing part 32, and the other side is connected with the power part 34, so that the shearing part 32 and the power part 34 are separated inside and outside the culture tank. The cross section of the spacer bush 31 is larger than that of the shearing part 32, so that the shearing device 3 can be simply, conveniently and rapidly arranged on the culture tank by directly inserting the spacer bush into the shearing part 32 through the mounting hole.

In this embodiment, be provided with the shearing mechanism 3 that can cut plant tissue in the culture tank, still set up spacer 31 between shearing mechanism 32 and power portion 34 simultaneously for shearing mechanism 3 dispersedly installs in the diapire 11 of culture tank, outside, thereby both can maintain and promote plant tissue's cultivation efficiency through the length control of shearing mechanism 3, can also avoid germ or impurity to get into in the culture tank through shearing mechanism 3, improved the leakproofness of culture tank.

The spacer 31 protrudes from the surface of the bottom wall 11 toward the tank cavity 90 along the vertical direction of the bottom wall 11 of the culture tank. The spacer 31 is formed with a concave groove on the other side corresponding to the position where the spacer 31 protrudes toward the tank cavity 90 as seen from the outside of the bottom wall 11 of the culture tank.

The shearing part 32 is connected with the outer convex surface of the spacer bush 31, and the power part 34 is arranged at the concave groove. Preferably, the shearing part 32 is provided with a member which is sleeved on the periphery of the spacer 31 and can rotate relative to the spacer 31, and the power part 34 attracts and drives the member from the other side of the spacer 31 through the spacer 31, so as to drive the shearing part 32 to shear plant tissues.

In this embodiment, by optimizing the shape of the spacer 31, the spacer 31 protrudes into the cavity 90 of the tank body, so as to enhance the attraction and driving effect of the power portion 34 on the shearing portion 32, ensure that the shearing device 3 shears plant tissue better, avoid plant tissue winding and agglomerating, and promote faster growth of plant tissue.

The spacer 31 may be provided with an annular groove recessed in the direction of the central axis thereof on the outer circumference, and the middle portion of the spacer 31 is located between the groove bottom and the groove top in the direction of the central axis of the annular groove.

Preferably, the middle part of spacer 31 is flush with the top of the tank, i.e. the middle part of spacer 31 is flush with the surface of bottom wall 11 of the culture tank.

Specifically, when the spacer 31 is provided on the culture tank, the annular groove of the spacer 31 extends from the surface of the bottom wall 11 toward the tank cavity 90 along the perpendicular direction of the bottom wall 11 of the culture tank, and the notch of the annular groove is located on the surface of the bottom wall 11 of the culture tank, so that the spacer 31 forms an annular structure with the annular groove.

Preferably, the middle part of the spacer 31 protrudes toward the tank cavity 90 to form a cylindrical structure with a cylindrical groove. In this embodiment, by optimizing the shape of the spacer 31, the spacer 31 protrudes into the cavity 90 of the tank body, so as to enhance the attraction and driving effect of the power portion 34 on the shearing portion 32, ensure that the shearing device 3 shears plant tissue better, avoid plant tissue winding and agglomerating, and promote faster growth of plant tissue.

The shearing part 32 comprises a first blade 323 and a second blade 324 which are respectively connected with the power part 34, the first blade 323 and the second blade 324 are arranged in a pasting way, and can be driven by the power part 34 to rotate oppositely or reversely to open and close so as to cut plant tissues.

The shearing portion 32 includes a pin 321 connected in series with the first blade 323 and the second blade 324, and the second blade 324 rotates relative to the first blade 323 with the pin 321 as a central axis, and forms a notch by the sides of the first blade 323 and the second blade 324.

The first blade 323 and the second blade 324 are elongated, the pin shaft 321 is vertically inserted in the centers of the first blade 323 and the second blade 324, and the second blade 324 rotates relative to the first blade 323 to form two symmetrical notches relative to the pin shaft 321.

In another connection manner, the shearing portion 32 includes a plurality of blade groups 322 connected in series to the pin 321, where each blade group 322 is formed by matching the first blade 323 and the second blade 324 in pairs and is distributed at equal intervals along the axis direction of the pin 321, so as to form a multi-layer structure. The spacing between adjacent blade sets 322 is between 1cm and 2cm, preferably between 1cm and 1.5 cm.

Specifically, all the first blades 323 in the blade set 322 are parallel to each other and are fixed with the pin shaft 321, all the second blades 324 are parallel to each other and are connected at the ends of the second blades 324 in the length direction of the second blades to form a frame body coated on the outer side of the first blades 323, and the second blades 324 rotate relative to the first blades 323 to form a continuously opened and closed notch.

In this way, all the second blades 324 in the blade groups 322 are connected together through the frame body, when the second blades 324 are driven to rotate relative to the first blades 323, the second blades 324 in the adjacent blade groups 322 rotate relative to the respective first blades 323 together to form adjacent continuously open and close cuts, and simultaneously, the plant tissues entering the cutting parts 32 are cut, so that the lengths of the cut plant tissues are consistent with the intervals between the adjacent blade groups 322.

In this embodiment, the shearing portion 32 is provided with a plurality of groups of blades, and the blade groups 322 are arranged at fixed intervals, so that plant tissues with consistent lengths can be obtained by cutting the plant tissues, the plant tissues in the culture tank have the same growth state, the nutrient content of the nutrient solution can be conveniently and regularly adjusted, the plant tissues can be trimmed, the nutrient solution circulation in the culture tank is promoted, stable growth conditions are maintained, and the cultivation efficiency and the quality of the plant tissues are improved.

The second blade 324 includes a rotating end 325 and a blade end 326, one end of the blade end 326 is connected to the rotating end 325, and the other end extends in a diameter direction of the rotating end 325 and is suspended at an outer circumference to form a cantilever shape. The rotating end 325 is sleeved on the pin 321, and when the second blade 324 rotates relative to the first blade 323, the blade end 326 can rotate around the pin 321 to form a continuously opened and closed notch.

The first blade 323 has the same structure and shape as the second blade 324.

Wherein the blade end 326 includes a closed side 327 for coining and an open side 328 opposite the closed side 327. The closed side 327 and the open side 328 extend radially from the rotating end 325 to the other end and gradually converge toward each other.

The ends of the closed side 327 are located on the same diameter line, and the closed side 327 extends from the rotational end 325 to the other end along an arc. Finally, the closed side 327 of the blade end 326 is formed with an inwardly concave recess.

The slit gradually closes from both ends of the closing side 327 toward the notch when the second blade 324 rotates relative to the first blade 323.

Further, the second blade 324 includes a plurality of the blade ends 326 evenly distributed along the outer circumference of the rotating end 325. For example, three blade ends 326 are provided at intervals of 120 ° at the outer periphery of the rotating end 325; four blade ends 326 are provided at the outer circumference of the rotating end 325 at 90 intervals.

Preferably, the second blade 324 is provided with two blade ends 326 symmetrically distributed about the rotational end 325.

In particular, the deployment side 328 extends along an arc from the rotational end 325 to the outer periphery, and the closure side 327 also extends along an arc from the rotational end 325 to the outer periphery. And, the open side 328 and the closed side 327 gradually converge at a point.

The second blade 324 is S-shaped and is formed of two blade ends 326 connected to a rotating end 325 and disposed at 180 ° intervals.

The blade end 326 is curved upwardly in a circumferential direction from the closed side 327 to the open side 328.

Preferably, the blade end 326 is configured as a spiral surface along the diameter of the rotating end 325.

Preferably, the first blade 323 has an elongated shape, and the first blade 323 is provided at both ends with a sharp corner inclined to the diameter thereof, and a side of the sharp corner opposite to the closed side 327 is recessed toward the first blade 323.

The central axis of the power part 34 coincides with the central axis of the shearing part 32, and the shearing part 32 is driven to shear plant tissues.

The power unit 34 is provided with a rotor that magnetically drives the shear unit 32 to open and close the slit through the bottom wall 11. Specifically, a magnetic attraction piece made of a magnetic material is fixedly arranged on the rotor. The magnetic attraction member may attract the cutout portion 32 via the bottom wall 11 and follow the rotation of the rotor around the central axis of the power portion 34.

In this embodiment, the power portion 34 is set to magnetically drive the shearing portion 32 to work, so that a traditional rotating shaft connection structure is omitted, and a sealing structure is not required to be arranged between the culture tank and the power portion 34, so that not only is the sealing performance of the bottom wall 11 improved, but also greasy dirt or germs of the power portion 34 can be completely prevented from entering the culture tank, a sterile environment in the culture tank can be kept for a long time, a stable and reliable environment is provided for growth of plant tissues, and further yield of the plant tissues and quality of the plant tissues in the same culture period are greatly improved.

The power part 34 is provided with a magnetic attraction member rotatable around its central axis. The center axis of the power portion 34 is disposed coincident with the center axis of the second blade 324. The magnetic attraction member restrains the second blade 324 with a magnetic field across the bottom wall 11 and rotates together.

Alternatively, the power section 34 is provided with a stator and a rotor that is drivable by the stator. The stator is fixed to the outer side surface of the bottom wall 11. The rotor and the stator are mutually sleeved and matched, and a gap is reserved between the rotor and the stator. The rotor is energized by an energy source to excite the stator to apply a torque to the rotor relative to the central axis, thereby driving the rotor to variably rotate about the stator.

It is understood that the energy source includes an electrical power source, a high pressure gas or a high pressure liquid, and the like.

In the power section 34, the rotor is provided in a ring shape. And the stator is provided as a cylinder and the diameter of the stator is smaller than the diameter of the rotor annular hole. The rotor is sleeved on the periphery of the stator.

The magnetic attraction member is fixed to an end face of the rotor facing the bottom wall 11. The second blade 324 has an iron piece on its outer periphery that is easily attracted. The magnetic attraction member rotates around the normal of the second blade 324 against the bottom wall 11 and attracts the second blade 324 to rotate together.

Alternatively, the power section 34 may also include a stator and a rotor. The stator is provided in a ring shape and is made of a ferromagnetic material. The middle part of the stator is provided with a mounting hole which penetrates through two ends along the central axis of the stator.

The rotor is provided in a long rod shape. The rotor is sleeved in the mounting hole of the stator, and the rotor can rotate freely in the mounting hole of the stator. The magnetic attraction member is fixed in the rotor at an end facing the bottom wall 11.

Preferably, one end of the rotor is wound with a coil, and the other end is connected with the magnetic attraction piece. The magnetic attraction piece is cylindrical. The second blade 324 is further provided with a cylindrical transmission part 33, one end of the transmission part 33 is connected with the second blade 324, and the other end of the transmission part is sleeved on the periphery of the magnetic attraction piece.

Alternatively, the power section 34 includes a stator and a rotor. The rotor is wound with a plurality of coils. Energizing the coil creates a magnetic pole on the rotor that rotates about its central axis.

Specifically, the plurality of coils are uniformly distributed centering on the rotor, and only one of the coils is energized. Then, the energization is switched between the coils in a clockwise or counterclockwise order, so that a magnetic pole whose direction is continuously changed around its central axis can be generated on the rotor.

The stator is made of a magnetic material and generates a magnetic field with stable magnetic poles. The rotor is electrified to generate rotating magnetic poles, and then the rotor is driven to rotate relative to the stator by attraction force and repulsion force between the magnetic poles.

In this embodiment, a coil is provided on the rotor, and a magnetic pole can be generated by energizing the rotor. The coil energized is switched synchronously by the rotation of the rotor by the same pole repulsion and different pole attraction, so that the magnetic poles on the rotor are always inconsistent with the magnetic poles of the rotor, and the torque applied to the rotor by the stator is continuously acted, thereby greatly improving the function conversion efficiency of the power part 34.

Alternatively, the power section 34 includes a stator and a rotor, and the high pressure medium flows between the stator and the rotor and applies work to the rotor, causing the rotor to rotate relative to the stator.

Specifically, the stator is provided with a passage for conveying a high-pressure medium and a plurality of chambers. The passage communicates with all of the chambers and can convey high pressure medium between the chambers. At least a portion of the rotor is positioned between the chambers and conceals the passageways. The high pressure medium flows from the passage to the adjacent chamber and may exert a force on the rotor. The rotor is pushed by the high-pressure medium to rotate relative to the stator.

The high pressure medium pushes the stator from one chamber into the other and loses a portion of the pressure. The pressure is converted into kinetic energy of the rotor.

Preferably, the high pressure medium comprises a high pressure gas or a high pressure liquid.

The transmission part 33 is arranged between the shearing part 32 and the power part 34, one end of the transmission part 33 is connected with the shearing part 32, and the other end is arranged close to the power part 34. The power part 34 transmits torque to the transmission part 33 through magnetic force and can drive the transmission part 33 to rotate around the pin 321.

The second blade 324 is connected to the inner circumferential surface of the transmission part 33 at a longitudinal end.

Specifically, the transmission portion 33 has a cylindrical shape, and a center portion thereof has a hole penetrating both ends along a center axis thereof. The second blade 324 is inserted into the hole of the transmission part 33. The second blade 324 is connected with both ends thereof to the inner surface of the hole, and the length direction of the second blade 324 is parallel to the diameter of the hole.

In this embodiment, the cutting portion 32 is embedded in the hole of the transmission portion 33, and the transmission portion 33 is connected with the second blade 324 and isolated from the first blade 323, so that the second blade 324 can be driven to rotate relative to the first blade 323, and the plant tissue can be pushed to circulate along the axial direction of the transmission portion 33, thereby avoiding the plant tissue from being repeatedly cut, being beneficial to ensuring the length of the plant tissue and improving the quality of the plant tissue.

The shearing part 32 and the spacer 31 are arranged at intervals along the axial direction of the pin 321. The end face of the shearing part 32 and the end face of the spacer 31 together with the inner peripheral surface of the transmission part 33 enclose a chamber for accommodating materials.

The transmission part 33 is further provided with a discharge opening 333, and the discharge opening 333 is provided on a side wall of the transmission part 33 and penetrates the transmission part 33 in a radial direction. The transmission part 33 may be provided with a plurality of discharge ports 333. The discharge openings 333 are uniformly distributed on the side wall of the transmission part 33 in the circumferential direction.

In particular, in the axial direction of the transmission portion 33, the discharge opening 333 is located between the shearing portion 32 and the spacer 31. I.e. the length of the discharge opening 333 in the axial direction of the transmission part 33 is smaller than the distance between the shearing part 32 and the spacer 31.

The transmission part 33 is formed by abutting the discharge section 331 and the driving section 332.

Specifically, the discharge section 331 is sleeved on the pin 321. And the discharge section 331 is provided with said discharge opening 333 on the side wall. The driving section 332 is sleeved outside the spacer 31. And one end of the driving section 332 is flush with the top surface of the spacer 31.

Further, in order to avoid the plant tissue from being blocked by the side wall of the transmission part 33, the plant tissue is quickened to flow out from the discharge hole 333 after being cut by the cutting part 32. A plurality of discharge openings 333 are provided uniformly distributed on the side wall of the discharge section 331 in the circumferential direction. In particular, the end of the second blade 324 is connected to the side wall between adjacent discharge openings 333 so that the cut-out on the cutout 32 is aligned with the discharge openings 333.

The guide cover 35 is made of a thin-walled material and is sleeved on the periphery of the shearing part 32.

The air guide sleeve 35 is also provided with an inlet 351 and a mounting opening 352 which are mutually parallel. And the inlet 351 and the mounting 352 are parallel to the circular surface formed when the blade rotates.

In this embodiment, the dome 35 is disposed on the outer periphery of the shearing portion 32, so that the plant tissue can flow from one side to the other side of the shearing portion 32, the circulating flow speed of the plant tissue is increased, the circulating flow area of the nutrient solution in the culture tank can be increased and promoted, the cut plant tissue can be ensured to flow from the shearing portion 32 to other areas rapidly, the plant tissue can be prevented from circulating in a small range near the shearing portion 32, the repeated cutting rate of the plant tissue is reduced, the problem of uneven length of the plant tissue is prevented, and the quality of the plant tissue in the culture tank can be effectively improved.

The spacer 31 penetrates into the pod 35 from the mounting port 352 of the pod 35 and is connected to the shear portion 32. Accordingly, the inlet 351 of the pod 35 is located on the side of the shear 32 facing away from the cup 31 in the axial direction of the shear 32.

In this embodiment, the air guide sleeve 35 is sleeved outside the spacer 31, and the air guide sleeve 35 can be simply, conveniently and rapidly arranged on the culture tank by dismounting the spacer 31, so that the air guide sleeve 35 is convenient to maintain and replace.

In addition, spacer 31 includes cylindrical boss 311. The air guide sleeve 35 is provided with a cylindrical hollow sleeve body and is sleeved on the outer peripheral surface of the convex column 311. A space is provided between the cover surface of the air guide cover 35 and the outer surface of the convex column 311. The space forms an annular channel.

The spacer 31 is further provided with a flange 312 extending outwardly from the end edge of the boss 311. The flange 312 is annular. The pod 35 is coupled to the flange 312.

Preferably, the pod 35 is coupled to the outer annular edge of the flange 312.

The air guide sleeve 35 is provided as a cylindrical thin-walled cylinder. The pod 35 is also provided with cavities penetrating both ends in the direction of the central axis thereof. The inlet 351 and the mounting opening 352 are communicated with the cavity and are respectively positioned at two ends of the thin-walled cylinder.

Further, an outlet 353 is provided on the sidewall of the air guide sleeve 35. When the pod 35 is sleeved on the cutout 32, one end of the outlet 353 is aligned with the cutout 32 and the other end is adjacent to the mounting port 352 in the axial direction.

The inlet 351 directs plant tissue into the incision of the shearing apparatus 3 and the outlet 353 is used to discharge sheared plant tissue into the culture tank.

In this embodiment, the dome 35 is disposed on the outer periphery of the shearing portion 32, so that the circulating flow area of the nutrient solution in the culture tank is increased, the plant tissue is promoted to circulate in the tank cavity 90 in a large range, the plant tissue in the area near the shearing portion 32 is prevented from being influenced by the shearing portion 32 to circulate in a small range, and the culture tank is ensured to be in a good circulating state, so that the plant tissue can be trimmed.

It should be noted that, when the plant tissue enters the shearing device 3 under the action of the air flow, the blades in the shearing device 3 rotate to generate vortex suction force, the plant tissue is sucked into the notch of the shearing device 3 from the inlet 351 of the air guide sleeve 35, and the regularly arranged plant tissue is sheared by the blades, so that the plant tissue is sheared into small segments meeting the requirements, and then is discharged from the outlet 353 of the air guide sleeve 35, so that the plant tissue is prevented from agglomerating and winding together, the shearing effect is affected, and smooth discharging is ensured.

In the embodiment of the present invention, as shown in fig. 1 to 3, the tank 1 includes an intake manifold 8, and high-temperature steam may be introduced into the intake device 70 through the intake manifold 8, thereby inputting the high-temperature steam into the tank 1 for cleaning and steam sterilization.

When the culture tank is cleaned and steam sterilized, water is introduced into the culture tank through the water inlet 9 at the top of the culture tank, then steam is introduced through the air inlet device 70, high-temperature steam is dispersed in nutrient solution through the air inlet device 70 to form a large number of small bubbles, and the small bubbles roll in the water, so that the contact area between the high-temperature steam and the water in the culture tank is greatly increased, and better cleaning and steam sterilization are achieved for the culture tank.

After the culture solution is added into the culture tank, high-temperature steam is introduced into the culture tube through the air inlet device 70, and a large number of small bubbles are formed in the culture solution due to the effect of the air inlet device 70, so that the contact area between the high-temperature steam and the culture solution is greatly increased, the temperature of the culture solution can be increased more quickly, and the disinfection efficiency and effect of the culture device are improved.

Further, the supply unit A1 further includes: a plurality of medium recovery pipelines; the conveying unit A2 further includes: the medium recovery pipelines are in one-to-one correspondence with the medium output branch pipelines, and the medium in the jacket 2 is led into the medium recovery pipelines through the medium output pipelines;

the plurality of output branch pipes are alternatively communicated with the second main pipe 22 to lead out the medium in the jacket 2, and each output branch pipe is controllably communicated with the second main pipe 22.

In the embodiment of the invention, the medium recovery pipeline comprises a hot water recovery main pipe E100, a chilled water recovery main pipe C100 and a cooling water recovery main pipe D100 which are arranged in parallel, and a steam recovery main pipe which is used for recovering the medium output by the jacket 2 through an output pipeline;

the plurality of output branch lines includes: chilled water recycling branch pipe C10; a cooling water recovery branch pipe D10; a hot water recovery branch pipe E10; and the steam is recycled and branched.

Specifically, the hot water recovery branch pipe E10 is connected to the hot water recovery main pipe E100, and the hot water introduced from the jacket 2 to the second main pipe 22 is introduced into the hot water recovery main pipe E100. The hot water supply main pipe E200 outputs hot water, enters the first main pipe 21 through the hot water supply branch pipe E20, then enters the jacket 2 for reference and temperature adjustment, and enters the hot water recovery branch pipe E10 through the second main pipe 22, and then returns to the hot water recovery main pipe E100 to form a temperature rise adjustment circulation loop.

Specifically, the steam recovery branch pipe is connected to the steam recovery main pipe, and high-temperature steam introduced from the jacket 2 to the second main pipe 22 is introduced into the steam recovery main pipe. High-temperature steam is output from the steam supply main pipe B100, enters the first main pipeline 21 through the steam supply branch pipe, enters the jacket 2 for temperature adjustment, enters the steam recovery branch pipe through the second main pipeline 22, and returns to the steam recovery main pipe to form a temperature-increasing adjustment circulation loop.

Specifically, the chilled water supply branch pipe C10 is connected to the chilled water supply main pipe C100, and chilled water supplied from the jacket 2 to the second main pipe 22 is supplied to the chilled water supply main pipe C100. Chilled water is output from the chilled water supply main pipe C200, enters the first main pipe 21 through the chilled water supply branch pipe C20, then enters the jacket 2 for reference and temperature adjustment, enters the chilled water recovery branch pipe C10 through the second main pipe 22, and then returns to the chilled water recovery main pipe C100 to form a cooling adjustment circulation loop.

Specifically, the cooling water recovery branch pipe D10 is connected to the cooling water recovery main pipe D100, and the cooling water introduced from the jacket 2 to the second main pipe 22 is introduced into the cooling water recovery main pipe D100. Cooling water is output from the cooling water supply main pipe D200, enters the first main pipe 21 through the cooling water supply branch pipe D20, then enters the jacket 2 for reference and temperature adjustment, enters the cooling water recovery branch pipe D10 through the second main pipe 22, and then returns to the cooling water recovery main pipe D100 to form a cooling adjustment circulation loop.

In this embodiment, a plurality of input branch pipes introduce different mediums into the jacket 2 through one main pipe, so that different mediums are introduced into the jacket 2, and the number of inlets on the jacket 2 is reduced. Different mediums in the jacket 2 are led out through the same main pipeline, and then corresponding output branch pipelines are selected according to specific mediums to be led into corresponding recovery main pipes, so that the different mediums are recovered in a distinguishing mode, and the number of medium outlets on the jacket 2 is reduced.

In another embodiment, the plant tissue culture device capable of automatically cleaning and adjusting temperature further comprises a waste liquid pipeline, the output branch pipeline further comprises a waste liquid output branch pipeline, and the waste liquid output branch pipeline is communicated with the waste liquid pipeline and is used for discharging waste liquid generated in the jacket 2 or a non-recyclable temperature adjusting medium into the waste liquid pipeline and is used for discharging the waste liquid. That is, when the waste liquid in the jacket 2 is required to be led out or the medium led out from the jacket 2 cannot be recycled, the waste liquid output branch pipeline is opened, the waste liquid in the waste liquid output branch pipeline is led into the waste liquid pipeline, and the waste liquid is discharged to the waste liquid treatment unit through the waste liquid pipeline for waste liquid treatment.

Further, the input branch pipeline and the output branch pipeline are both provided with an on-off valve 5, and the on-off control of the on-off valve 5 controls the input branch pipeline communicated with the first main pipeline 21 and/or the output branch pipeline communicated with the second main pipeline 22.

In the embodiment, the bottom of the on-off valve 5 is provided with a sterilizing medium input port for introducing high-temperature steam into the valve body, so as to realize sterilization of the medium in the valve body of the on-off valve 5 and the pipeline.

Specifically, on-off valves 5 are provided on all the input branch lines and the output branch lines, and the on-off valves 5 include manual valves and +.

Or a pneumatic valve, if both a manual valve and a pneumatic valve are included, the manual valve and the pneumatic valve are connected in series. The corresponding valve bodies can be opened as required, and the valve bodies on the branches which do not need to work are kept closed. When the valve body is opened, a high-temperature steam pipeline on the valve body is opened, and high-temperature steam is introduced into the opened valve body, so that the sterilization effect of the valve body and branch pipelines is ensured, and the sterility of a medium input into the jacket 2 is ensured.

In the embodiment of the invention, the tank 1 is provided with a temperature monitoring device for monitoring the culture temperature in the tank 1, and the culture temperature in the tank 1 is adjusted by selecting different media for input according to the monitored temperature.

Specifically, a temperature monitoring device is arranged at the position of the lower part of the tank body 1, which is not provided with the jacket 2, and is used for monitoring the culture temperature in the tank body 1, and the culture temperature in the tank body 1 can be monitored in real time to judge which medium needs to be input so as to ensure the stability of the culture temperature in the tank body 1 and promote the rapid growth of plant tissues.

In the embodiment of the invention, the lower edge of the jacket 2 is provided with a medium inlet which is communicated with the medium input pipeline and is used for guiding the temperature-adjusting medium in the medium input pipeline into the jacket 2. The upper edge of the jacket 2, which is back to the medium inlet, is provided with a medium outlet which is communicated with the medium output pipeline and used for guiding the medium in the jacket 2 into the medium output pipeline.

Specifically, a medium inlet is arranged at the lower edge of the jacket 2 and matched with the first main pipeline 21, a medium outlet is arranged at the upper edge of the opposite side of the medium inlet and matched with the second main pipeline 22, so that a medium loop is formed, the arrangement of an opening on the jacket 2 is reduced, and the input of a proper medium is ensured.

Specifically, the temperature monitoring device is used for detecting the culture temperature in the plant tissue culture device tank body 1, then a proper heat transfer medium is selected according to the monitored temperature and is input into the jacket 2, the on-off valve 5 on the corresponding input branch pipeline and the on-off valve 5 on the corresponding output branch pipeline are opened according to the selected medium, the selected medium is input from the input branch pipeline, the medium runs in the jacket 2, and is discharged from the corresponding output branch pipeline, so that the temperature regulation of the plant tissue culture tank is realized, and meanwhile, the circulation of the temperature regulating medium is realized.

In another embodiment, when the medium in the jacket 2 is not recycled during discharging, the waste liquid output branch pipeline is opened, and the waste liquid output branch pipeline is connected with the waste liquid pipeline, at this time, the used medium in the jacket 2 is not fed into the medium recovery main pipe, but is led into the waste liquid pipeline through the waste liquid output branch pipeline, and then the waste liquid treatment is carried out after the temperature and the pressure of the waste liquid treatment part are reduced.

In another embodiment of the present invention, as shown in fig. 1 to 3, the cultivation tank of the plant cultivation apparatus includes a seed tank and a fermenter, and the seed tank and the fermenter of the plant cultivation apparatus may be made of any materials suitable for preparing the fermenter, such as glass, stainless steel, high temperature resistant plastic, etc., which can be used for high temperature sterilization; preferably stainless steel, durable and long in service life.

For convenience of distinction, a small-sized plant tissue culture tank is called a seed tank, and a large-sized plant tissue culture tank is called a fermenter.

In order to reduce the number of times of inoculating adventitious roots, adventitious buds, etc. to a culture apparatus and to achieve the purpose of multiple cycle proliferation of adventitious roots, adventitious buds, etc., a plant tissue culture tank for culturing isolated plant tissues or cells is separately provided as a seed tank and a fermentation tank.

The seed tank is used for inoculating adventitious roots and adventitious buds and is used as seeds for rapidly breeding the adventitious roots and the adventitious buds in the next period of the fermentation tank.

In particular, the volume of the seed tank is smaller than the volume of the fermenter and is 1/3 to 1/5 of the volume of the fermenter.

Specifically, a seed transfer pipeline and a seed returning pipeline are arranged between the seed tank and the fermentation tank, and the seed transfer pipeline is used for guiding culture solution from the seed tank into the fermentation tank through a connecting port arranged on the fermentation tank, and continuously culturing the culture solution; the seed returning pipeline guides the culture solution which is transferred from the fermentation tank to the seed tank into the seed tank through a connecting port arranged on the seed tank.

Specifically, the outer side of the peripheral wall of the tank body 1 is provided with a jacket 2, the upper side of the jacket 2 is positioned below the connecting port, the lower side of the jacket 2 extends to the inclined bottom wall 11 and is positioned above the air inlet device 70 and the shearing device 3, and a temperature regulating medium can be introduced into the jacket 2 to regulate the culture temperature in the tank body 1 within a proper temperature range required by culture.

As shown in fig. 10, at least two air inlet devices 70 and two shearing devices 3 are arranged on the bottom wall 11 of the seed tank. The shearing devices 3 and the air inlet devices 70 are alternately distributed at intervals on the bottom wall 11, and the installation position of the shearing devices 3 is higher than the air inlet devices 70 in the vertical direction.

Preferably, the line connecting the mounting positions of the two shearing devices 3 and the line connecting the mounting positions of the two air inlet devices 70 pass through the center of the seed tank, respectively.

The aeration sections 72 of the two air inlet devices 70 of the present invention are preferably arranged on the same plane when installed and the projections of the extending directions in the seed tank are approximately parallel, symmetrically arranged with respect to the center of the bioreactor.

As shown in fig. 10, the aeration portion 72 has an extended length, one end connected to the conduit 73 is spaced apart from the bottom wall 11, and the other end extends in a direction approaching the center of the bioreactor while being tangent or intersecting at the center line of the bioreactor. The distance between the end of the aeration portion 72 connected to the conduit 73 and the bottom wall 11 is smaller than the distance between the other end of the aeration portion 72 and the bottom wall 11. That is, the length of the conduit 73 extending into the culture tank is short, so that the aeration portion 72 can be mounted on the bottom wall 11 and the mounting angle can be adjusted.

The gas supplied from the aeration unit 72 has a certain pressure, and the culture solution and plant tissue in the bioreactor are circulated along the outer parabolic path which is raised near the central axis and then spread to the peripheral side, and lowered near the inner wall of the bioreactor, and the culture solution is applied with a certain force to the plant tissue in the process of raising and lowering along the path near the parabolic path so as to make the plant tissue basically arranged and flowed in a regular manner along the length direction along the parabolic path.

The shearing device 3 has a gap between the ends extending toward the central line of the bioreactor, and the gap is used for passing the culture solution and plant tissues rising near the central line.

Plant tissue arranged in the same direction under the action of the parabolic air flow in the bioreactor flows downwards into the incision of the shearing device 3.

The shearing device 3 and the air inlet device 70 adopt the arrangement mode, the evenly distributed air can be provided in the bioreactor, and the distribution ratio of the aeration part 72 at the central line of the bioreactor is higher than that of the bottom wall 11, so that the kinetic energy provided by the air at the central line area is higher than the potential energy and the upper pressure of the culture solution, the culture solution can move upwards far under the driving of air flow, when the culture solution moves to a certain height, the kinetic energy generated by the air on the culture solution is far away from the aeration part 72 and is weakened and smaller than the potential energy and the upper pressure of the culture solution, and meanwhile, the potential energy and the upper pressure of the culture solution at the inner wall are higher than the kinetic energy provided by the air, so that the culture solution can circularly move along the track of the outer paraboloid which is upwards and downwards along the area close to the inner wall of the bioreactor along the central line, and the descending track is close to the vertical descending along the reason of the gravity of the culture solution, on one hand, the contact area of the air, the culture solution and the plant tissue is further improved, the gas dissolution rate is prevented from rotting, and an excessive stirring device is not required; on the other hand, the culture solution generates driving force to plant tissues in the ascending and descending processes along the track approaching to the paraboloid, so that the plant tissues are regularly arranged towards the same direction, and the plant tissues are prevented from being wound together in the growing process, and the growing efficiency is prevented from being influenced; and the moving culture solution guides the plant tissues to be regularly arranged and flow into the incision of the shearing device 3, and simultaneously, the cutters in the shearing device 3 rotate to generate vortex suction force, so that the regularly arranged plant tissues are further sucked into the incision of the shearing device 3, the shearing uniformity is ensured, and the wound plant tissues are prevented from being sheared unevenly or repeatedly sheared.

Further, the included angle β between the shearing device 3 and the bottom wall 11 is adjustable, the aeration portion 72 and the shearing device 3 extend and intersect at a point along the respective length direction to form an included angle β1, the included angle between the bottom wall 11 and the horizontal plane is β2, and when β1+β2=β is satisfied, preferably, the included angle β is 90 degrees, the air inlet effect of the aeration portion 72 is optimal.

Also, as shown in fig. 11, at least four air inlet devices 70 and two shearing devices 3 are arranged on the bottom wall 11 of the fermenter in a similar manner to the seed tank.

The four air inlet devices 70 are uniformly arranged in the circumferential direction of the bottom wall 11 and are disposed at equal intervals. The aeration portion 72 of the air inlet device 70 is maintained horizontal and has a certain length. One end of any two opposite groups of aeration parts 72 connected with the guide pipe 73 is spaced from the bottom wall 11, the other end of the aeration parts is tangent or intersected at the central line of the bioreactor, and projections in the fermentation tank are approximately parallel and symmetrically arranged relative to the center of the bioreactor; the projections of adjacent sets of aeration sections 72 into the fermenter are approximately vertical.

Every two air inlet devices 70 are provided with a shearing device 3 at intervals, and the connecting line of the installation position of one shearing device 3 on the same side of the bottom wall 11 and the installation position of two adjacent air inlet devices 70 in the fermentation tank approximately forms an isosceles triangle, and the installation position of the shearing device 3 is higher than the air inlet devices 70 in the vertical direction.

By reasonably setting the relative positions of the shearing device 3 and the air inlet device 70, the air provided by the aeration part 72 of the air inlet device 70 promotes the culture solution and plant tissues in the bioreactor to rise along the area close to the central axis, then diffuse to the peripheral side and circularly move along the track of the outer paraboloid close to the inner wall of the bioreactor, and the culture solution exerts certain force on the plant tissues in the rising and falling processes along the track close to the paraboloid so as to enable the plant tissues to be basically regularly arranged and flow along the length direction along the track of the paraboloid.

Alternatively, the aeration unit 72 of the air inlet unit 70 is disposed close to the bottom wall 11, and the air supplied from the aeration unit 72 circulates the culture medium and plant tissue in the bioreactor along the track of the inner paraboloid which is raised near the inner wall of the bioreactor and lowered near the central axis.

In the process of ascending and descending along the track approaching to the paraboloid, the culture solution exerts certain force on plant tissues to enable the plant tissues to basically and regularly arrange and flow along the length direction along the track approaching to the paraboloid, so that the plant tissues are conveniently sheared into small sections by the shearing device 3, and the plant tissues are prevented from being repeatedly sheared together.

The shearing device 3 comprises a rotatable cutter into which the culture medium is guided in a regular array of plant tissue during descent along a trajectory approaching a parabolic shape.

The cutter comprises double cutters which are distributed up and down, and most plant tissues are sheared into the size of the interval between the double cutters.

An included angle is formed between the shearing device 3 and the bottom wall 11, and an included angle is formed between the shearing plane where the double knife is positioned and the moving track of the plant tissue along the parabolic descending.

In this embodiment, the included angle between the shearing device 3 and the bottom wall 11 is preferably 90 °, that is, the shearing device 3 is vertically disposed on the bottom wall 11, at this time, the shearing plane where the double knives are located and the track where the paraboloid type descends are acute angles, the culture solution guides the regularly arranged plant tissues into the cutters in the descending process along the track which is close to the paraboloid type, so, when the plant tissues are sheared into small sections by the shearing device 3 by using the cutters, the shearing surface is an inclined plane, the area of the shearing surface is increased, seeds of more plant tissues such as ginseng adventitious roots can be cultivated, the production efficiency is improved, the shearing uniformity is high, the wound plant tissues are prevented from being sheared unevenly or repeatedly, the production degree of the wound plant tissues is conveniently controlled uniformly by shearing into uniform small sections, the discharging is also convenient, and the wound plant tissues are prevented from blocking the pipeline.

Or, the installation angle of the shearing device 3 is adjusted, so that the shearing plane where the double knife is positioned is approximately perpendicular to the paraboloid-shaped descending track, plant tissues can be opposite to the incision of the shearing device 3, the number of the plant tissues entering the shearing device 3 each time is increased, the shearing efficiency is improved, and the plant tissues are ensured to be sheared into small sections with approximate length and uniformity.

Further, the temperature-adjusting medium outputted from the medium supply main pipe is divided into two branch pipes after passing through one main pipe, and is respectively connected to the seed tank and the input main pipe provided on the fermenter, namely, the first main pipe 21 described in the above embodiment.

Specifically, different temperature-adjusting mediums are output from the corresponding medium supply main pipes through the corresponding main pipelines and then respectively enter the corresponding temperature-adjusting medium branch pipelines, are converged to the input main pipelines for connecting the seed tank and the fermentation pipe after passing through the branch pipelines, are input into the corresponding jacket 2 through the respective input main pipelines, and play a role in adjusting the temperature of the seed tank and the fermentation tank.

More specifically, after different mediums are output from the main supply pipeline, the mediums are respectively input into the main input pipeline arranged on the seed tank and the fermentation tank through the two branch pipelines, and then enter the jacket 2 through the main input pipeline to regulate the temperature of the plant tissue culture tank; after flowing in the jacket 2, the temperature-adjusting medium is output through an output main pipeline arranged on the jacket 2, enters a corresponding medium recovery main pipe through a corresponding output branch pipeline, and is recovered or enters a waste liquid pipe to treat waste liquid.

Further, when the plant tissue culture device starts to culture, the on-off valve 5 on the steam branch pipe is opened, and steam is introduced into the jacket 2 of the tank body 1; in the culture process, on-off valves 5 on branch pipelines corresponding to the temperature-regulating mediums are opened as required, and the corresponding mediums are introduced to regulate the culture temperature in the tank body 1.

Specifically, before seeds are not placed in the culture solution in the culture device, firstly, steam is introduced into a jacket 2 on a tank body 1 and the tank body 1, an on-off valve 5 on a steam input branch pipeline is opened, high-temperature steam is introduced into the jacket 2, and the tank body 1 is heated up rapidly to a temperature suitable for plant tissue culture; opening an on-off valve 5 on an air inlet branch pipe 8, introducing high-temperature steam into the tank body 1 to disinfect the culture solution in the tank body 1, and introducing the high-temperature steam in the jacket 2 to assist the culture solution in the tank body 1 to disinfect while heating the tank body 1; then the on-off valve 5 on the high temperature steam branch pipe is closed, seeds are put into the tank body 1 of the seed tank, and the seeds are cultivated.

In the process of cultivation, the temperature monitoring device monitors the cultivation temperature in the tank body 1, and according to the change of the temperature, the on-off valve 5 on the corresponding temperature-adjusting medium branch pipeline is opened at any time, the temperature of the seed tank body 1 is adjusted, and the growth temperature of adventitious roots is ensured to be proper. When the culture tank is needed, steam is firstly introduced into the jacket 2 of the culture tank, so that the temperature of the culture tank is quickly increased to the temperature for seed culture, then seeds are transplanted into the culture tube from the seed tank, the temperature of the culture tank body 1 is monitored through the temperature monitoring device in the culture process, the on-off valve 5 on the corresponding heat transfer medium branch pipe is opened at any time according to the change of the temperature, the temperature of the culture tank body 1 is regulated, and the growth temperature of plant tissues is ensured to be proper.

When the on-off valve 5 of the input branch pipeline or the output branch pipeline is opened, a sterilizing medium is introduced into the on-off valve 5, and the on-off valve 5 and the introduced medium on the branch pipeline are sterilized.

In another embodiment of the present invention, a temperature adjustment method of a temperature adjustment system is provided, including steps S10 to S30.

In step S10, during the culturing process, the temperature monitoring device monitors the temperature T inside the culture tank;

in step S20, the monitored temperature T is equal to a preset threshold T ₀ Comparing;

in step S30, if the monitored temperature T exceeds the preset threshold T ₀ Opening on-off valves on the corresponding branch pipes, and introducing a temperature regulating medium;

wherein, a threshold T is preset ₀ Is a range value outside of which the predetermined threshold is exceeded.

Optionally, in step S30, if the temperature T is higher than the preset threshold T ₀ And opening on-off valves 5 on the chilled water supply branch pipe C20, the chilled water recovery branch pipe C10/the cooling water supply branch pipe D20 and the cooling water recovery branch pipe D10, and introducing chilled water/cooling water for cooling regulation.

Optionally, in step S30, if the temperature T is lower than the preset threshold T0, the on-off valves 5 on the hot water supply branch pipe E20 and the hot water recovery branch pipe E10/the steam supply branch pipe and the steam recovery branch pipe are opened, and hot water/high temperature steam water is introduced to perform temperature rise adjustment.

Specifically, in the process of plant cultivation, a temperature monitoring device arranged on the cultivation tank monitors the temperature in the tank body 1, compares the monitored temperature with a preset threshold value, and inputs different media to regulate the temperature according to the comparison result.

In this embodiment, the preset threshold is a temperature range suitable for plant cultivation.

Optionally, when the monitored temperature value is higher than the highest value of the range values of the preset threshold value, cooling by inputting chilled water or cooling water; when the monitored temperature is lower than the lowest value of the preset threshold value, the temperature is increased by inputting hot water or high-temperature steam.

Optionally, when temperature adjustment is performed, different mediums are introduced into the jacket 2, after the temperature adjustment is completed, the mediums do not return to the medium recovery main pipe, then the on-off valve 5 on the waste liquid output branch pipe is opened, after the temperature adjustment medium is input into the jacket 2 from the corresponding input branch pipe, the temperature adjustment medium flows, is discharged through the waste liquid output branch pipe, enters the waste liquid treatment unit, and performs waste liquid treatment.

In a second aspect of the present invention, a control method of a plant tissue culture apparatus for automatic cleaning and temperature adjustment is provided, and the method is applied to the plant tissue culture apparatus for automatic cleaning and temperature adjustment, and includes: steps S100 to S300.

In step S100, the cleaning system is controlled to feed water into the culture tank through a water inlet on the culture tank before adding culture solution to the culture tank, so as to clean the culture tank.

In step S200, after the washing is completed, the used water is discharged.

In step S300, steps S100 to S200 are repeated at least three times, and the culture medium and the plant tissue seeds are added to the culture tank to perform the culture.

Optionally, in step S100, at least: the control system controls the air inlet device arranged at the bottom of the culture tank to enter air, and simultaneously controls the shearing device to work, so that the shearing device is cleaned at the same time.

Optionally, in step S300, when the last cleaning is performed, the water inlet on the culture tank is controlled to feed water into the culture tank, the valve body of the air inlet branch pipe is controlled to be opened, and steam is introduced into the culture tank through the air inlet device, so that cleaning and steam sterilization are performed.

s301, adding culture solution into a culture tank;

In the embodiment of the invention, the culture tank is required to be cleaned before plant tissue culture is carried out, the control system controls the cleaning system to feed water into the culture tank through the water inlet 9 arranged in the culture tank, the culture tank is cleaned with water, and after the cleaning is finished, the cleaned water is discharged to finish the first cleaning of the culture tank. To ensure that no residue remains in the culture tank, the culture tank is cleaned at least three times.

It should be noted that, the process of cleaning the culture tank before plant tissue culture includes cleaning the culture tank when the culture tank is used for the first time, and cleaning the culture tank when the culture solution is replaced after the culture is completed once.

In the cleaning process, the control system controls the air inlet device 70 and the shearing device 3 to work, air is introduced from the air inlet device 70, tiny bubbles are formed in the air through the air inlet system, the culture tank can be better cleaned, meanwhile, the shearing device 3 is controlled to work, and the shearing device 3 can be cleaned at the same time, so that the culture tank can be cleaned in all directions.

In the embodiment of the invention, when the last cleaning is performed, the control system controls the cleaning system to feed water from the water inlet 9 of the culture tank, and simultaneously controls the high-temperature steam supply pipeline B100 to supply high-temperature steam, and the steam supplied by the high-temperature steam is input into the air inlet device 70 through the air inlet branch pipe 8 and then enters the culture tank through the air inlet device 70 to clean and sterilize the culture tank.

After the culture tank is cleaned and steam sterilized, a culture solution is added into the culture tank, and steam is introduced into the culture tank through the air inlet device 70 to steam sterilize the culture solution, so as to ensure that the culture solution is in a sterile culture state. The control system controls the temperature adjusting system to adjust the temperature, and inputs the corresponding temperature adjusting medium to reduce the temperature of the culture tank to the proper plant tissue culture temperature.

After the steam sterilization of the culture solution is completed, plant tissue seeds are added into the culture tank for culture, the temperature monitoring device arranged on the culture tank detects the temperature in the culture tank in the culture process, and the control system controls the temperature adjusting system to introduce a required medium into the jacket 2 according to the temperature monitoring result of the temperature monitoring device, so that the temperature of the culture tank is regulated, and the growth of the plant tissue seeds is ensured to be suitable in the plant culture process.

The foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited to the above-mentioned embodiment, but is not limited to the above-mentioned embodiment, and any simple modification, equivalent change and modification made by the technical matter of the present invention can be further combined or replaced by the equivalent embodiment without departing from the scope of the technical solution of the present invention.

Claims

1. A plant tissue culture apparatus for automatic cleaning and tempering, comprising:

2. The self-cleaning and tempering plant tissue culture apparatus according to claim 1, wherein,

the temperature-regulating water tank is provided with a water containing cavity, and the water containing cavity and the jacket are connected with the supply unit through the conveying unit to form a circulation loop; the conveying unit comprises a water inlet pipe and a water outlet pipe which are communicated with the supply unit and the water containing cavity; and a water pump is arranged in the water inlet pipe in series and used for conveying the water in the water containing cavity to the jacket.

3. The self-cleaning and tempering plant tissue culture apparatus according to claim 2, wherein,

the temperature adjustment system further includes: the main control unit is used for controlling the proportion of the chilled water and the high-temperature steam which are conveyed to the heat exchange structure of the temperature-adjusting water tank by the liquid inlet pipe; the heat exchange structure is arranged close to the water containing cavity.

4. The plant tissue culture apparatus according to claim 3, wherein the automatic washing and temperature adjustment device comprises,

the supply unit comprises a plurality of medium supply pipelines, wherein the medium supply pipelines comprise hot water supply main pipes which are arranged in parallel and are used for supplying a medium with a first temperature; a high-temperature steam supply main pipe for supplying a medium of a second temperature; a chilled water supply main pipe for supplying a medium of a third temperature; a cooling water supply pipe for supplying a medium of a fourth temperature;

the conveying unit comprises a plurality of medium input branch pipelines and a first main pipeline which are connected in parallel, and the medium input branch pipelines are alternatively communicated with the first main pipeline;

5. The plant tissue culture apparatus according to claim 4, wherein the high temperature steam supply line is provided with a plurality of branches, one of which is connected to an air intake branch pipe, the air intake branch pipe is connected to an air intake device, and steam is introduced into the culture device through the air intake device to clean and sterilize the culture device.

6. The self-cleaning and temperature regulating plant tissue culture apparatus according to claim 4,

the supply unit further includes: a plurality of medium recovery pipelines;

the conveying unit further includes: a second main pipe and a plurality of medium output branch pipelines,

the medium recovery pipelines are in one-to-one correspondence with the medium output branch pipelines, and the medium in the jacket is led into the medium recovery pipelines through the medium output branch pipelines;

a plurality of medium output branch pipelines are alternatively communicated with the second main pipeline to lead out the medium in the jacket; each of the output branch lines is controllably in communication with the second main line.

7. A method for controlling an automatic cleaning and tempering plant tissue culture apparatus according to any one of claims 1 to 6, the method comprising:

s100, before the culture solution is added into the culture tank, controlling a cleaning system to feed water into the culture tank through a water inlet on the culture tank so as to clean the culture tank;

s200, after the cleaning is finished, discharging used water;

s300, repeating the steps S100-S200 at least three times, and adding the culture solution and the plant tissue seeds into a culture tank for culture.

8. The control method according to claim 7, wherein,

in step S100, at least:

the control system controls the air inlet device arranged at the bottom of the culture tank to enter air, and simultaneously controls the shearing device to work so as to finish the simultaneous cleaning of the shearing device.

9. The control method according to claim 7, wherein,

in step S300, when the last cleaning is performed, the water inlet on the culture tank is controlled to feed water into the culture tank, the valve body of the air inlet branch pipe is controlled to be opened, and steam is introduced into the culture tank through the air inlet device, so that cleaning and steam sterilization are performed.

10. The control method according to claim 7, wherein,

in step S300, after the last cleaning is completed, the method includes:

s301, adding culture solution into a culture tank;

s303, controlling a temperature adjusting system to adjust the temperature of the culture device to an appropriate culture temperature;