CN117322346A

CN117322346A - Biological reaction device for plant tissue

Info

Publication number: CN117322346A
Application number: CN202311562599.3A
Authority: CN
Inventors: 张洪喜; 张明臣; 刘金坤; 高秀君; 刘冰
Original assignee: Shandong Anran Nanometre Ind Development Co ltd
Current assignee: Shandong Anran Nanometre Ind Development Co ltd
Priority date: 2023-11-22
Filing date: 2023-11-22
Publication date: 2024-01-02

Abstract

The invention discloses a biological reaction device of plant tissues, which comprises a culture device, a detection device and a control device, wherein the culture device is used for culturing the plant tissues; the heat transfer structure is a jacket sleeved on the outer peripheral wall of the culture device and used for controlling the temperature of the culture device, the heat transfer structure is provided with a notch, and the detection device is arranged on the outer wall of the culture device in the notch. According to the invention, plant tissues are cultivated by the cultivation device, and the temperature in the device is kept stable by the heat transfer structure, so that the influence of external temperature on the environment in the device is prevented. And the detection device is arranged on the outer wall of the culture device in the notch of the heat transfer structure, so that the damage or heat loss of the heat transfer structure caused by the fact that the detection device passes through the heat transfer structure can be avoided, the integrity of the heat transfer structure is ensured, the temperature is kept stable, and the plant tissue grows better.

Description

Biological reaction device for plant tissue

Technical Field

The invention belongs to the field of plant tissue culture devices, and particularly relates to a plant tissue biological reaction device.

Background

Along with the continuous improvement of living conditions, people generally pay more attention to strengthening physique through medicinal materials with nourishing effect by diet, and perform health care. Due to the huge population, the demand for medicinal plants has risen dramatically. In addition, the cultivation of medicinal plants by the traditional method not only requires a large amount of land and a long growth period, but also requires a proper climate. Any condition unsuitable limits and reduces the yield of medicinal plants.

Therefore, scientific workers develop a method and a culture device for large-scale cultivation by using isolated tissues or cells of plants. By combining the organs of plants: peeling roots, stems, leaves and the like, then placing the plant into a culture medium containing nutrient components for cultivation, simultaneously providing other environmental conditions such as temperature, illumination and the like suitable for growth, inducing organs of the plant into callus, adventitious buds and adventitious roots, finally, using the callus, the adventitious buds and the adventitious roots as seeds for cultivating the plant, and placing the plant into a cultivation device for cultivation.

In the mass production of plant tissues, the temperature control of the culture device is very important for the growth of plant tissues, especially adventitious roots, and the growth of plant tissues can be damaged due to overhigh or overlow temperature, uneven heating and the like. The detection device of the culture device in the prior art passes through the heat preservation layer in a sealing way, and the temperature is higher and the temperature change is large in some cases, so that the sealing damage can be caused, the heat transfer medium leakage and the detection device damage occur, the heat transferred at the sealing part is different from other positions, the temperature control in the culture device can be influenced, and the plant tissue growth and cultivation are slow.

The present invention has been made in view of this.

Disclosure of Invention

The technical problem to be solved by the invention is to overcome the defects in the prior art, and provide a plant tissue biological reaction device which is provided with a culture device and a heat transfer structure, wherein the heat transfer structure is provided with a notch, a detection device is arranged on the outer wall of the culture device which is exposed in the notch, the uneven heat transfer caused by the fact that the detection device passes through the heat transfer structure to be damaged in a sealing way can be avoided, and the heat transfer structure is more convenient to install.

In order to solve the technical problems, the invention adopts the basic conception of the technical scheme that: a biological reaction device of plant tissue comprises,

the culture device is used for culturing plant tissues, and a detection device is arranged on the peripheral wall of the culture device and is used for detecting parameters and/or sampling of a culture solution in the culture device;

the heat transfer structure is a jacket sleeved on the peripheral wall of the culture device and used for controlling the temperature of the culture device, a notch is formed in the lower end of the jacket and extends downwards to penetrate through the lower end edge of the jacket, and the detection device is arranged on the peripheral wall of the culture device in the notch.

Further, the culture device is provided with a first tank body and a second tank body which are communicated, the second tank body extends downwards from the lower end of the first tank body, and the inner diameter of the second tank body is gradually reduced from the inner diameter of the first tank body;

The heat transfer structure comprises a first annular sleeve and a second annular sleeve which are communicated, the peripheries of the connecting ends of the first tank body and the second tank body are correspondingly sleeved, and the notch extends upwards from the edge of the lower end of the second annular sleeve to be at least arranged on the second annular sleeve.

Further, the culture apparatus comprises a seed tank for cultivating plant tissue, the heat transfer structure is sleeved on the seed tank, the first annular sleeve and the second annular sleeve of the heat transfer structure are correspondingly sleeved on the periphery of the connecting end of the first tank body and the second tank body of the seed tank, and the notch of the heat transfer structure extends upwards from the lower end edge of the second annular sleeve to the first annular sleeve.

Further, the first annular sleeve of the seed tank extends from the upper part of the first tank body of the seed tank to the edge of the first tank body connected with the second tank body; the second annular sleeve of the seed tank extends downwards from the lower end of the first annular sleeve to slightly exceed the connecting edge of the second tank body and the first tank body, and the thickness of the second annular sleeve of the seed tank gradually increases from top to bottom;

the notch of the heat transfer structure on the seed tank extends upwards from the edge of the lower end of the second annular sleeve on the seed tank to be more than half of the axial extension length of the first annular sleeve.

Further, the culture apparatus further comprises a culture tank for secondarily culturing the plant tissue cultured in the seed tank, wherein the culture tank is in circulation communication with the seed tank, the heat transfer structure is sleeved on the culture tank, the first annular sleeve and the second annular sleeve of the heat transfer structure are correspondingly sleeved on the periphery of the connecting end of the first tank body and the second tank body of the culture tank, the maximum inner diameter of the first tank body of the culture tank is far greater than the maximum inner diameter of the first tank body of the seed tank, and the notch of the heat transfer structure is formed by upwards extending the lower end edge of the second annular sleeve on the second annular sleeve.

Further, the first annular sleeve of the culture tank extends from the upper part of the first tank body of the culture tank to the edge of the first tank body connected with the second tank body;

the second annular sleeve of the culture tank extends downwards from the lower end of the first annular sleeve along the second tank body to exceed half of the axial extension length of the second tank body;

the notch of the heat transfer structure on the culture tank extends upwards from the edge of the lower end of the second annular sleeve on the culture tank to be more than half of the axial extension length of the second annular sleeve.

Further, the second tank body at the bottom of the seed tank is provided with at least two groups of air inlet structures for supplying air into the tank, and the air inlet structures are positioned below the heat transfer structure of the seed tank; the air inlet structure of the seed tank is oppositely arranged on the peripheral wall of the second tank body of the seed tank;

The second tank body at the bottom of the culture tank is provided with at least four groups of air inlet structures for supplying air into the tank, and the air inlet structures are positioned below the heat transfer structure of the culture tank; the inlet structure of the culture tank is circumferentially and uniformly distributed on the outer peripheral wall of the second tank body of the culture tank.

Further, two groups of air inlet structures of the seed tank extend into the second tank body, and an air inlet rod which is horizontal and has a certain length is arranged at the part of the two groups of air inlet structures extending into the tank, so that sterile air is conveyed into the second tank body in a bubble mode; the seed tank is characterized in that one group of air inlet structures of the seed tank are arranged right below the notch, and the other group of air inlet structures are symmetrically arranged with the air inlet structures below the notch.

Further, the four groups of air inlet structures of the culture tank extend upwards obliquely into the second tank body; the part of the air inlet structure extending into the tank is provided with an air inlet rod which is kept horizontal and has a certain length, and sterile air is conveyed into the second tank body of the culture tank in a bubble mode; the projections of any two opposite groups of air inlet rods in the tank body of the culture tank are approximately parallel, and the projections of two adjacent groups of air inlet rods in the tank body are approximately vertical;

One group of air inlet structures of the culture tank are arranged right below the notch, and the air inlet structures are circumferentially arranged on the second tank body of the culture tank at equal intervals.

Further, at least two groups of cutting structures are arranged on the seed tank, and the air inlet structure on the seed tank and the cutting structures are arranged at intervals;

at least two groups of cutting structures are arranged on the culture tank, the connecting lines of the mounting positions of the cutting structures on the culture tank and the mounting positions of the adjacent two air inlet structures approximately form an isosceles triangle, and the mounting positions of the cutting structures are higher than the air inlet structures in the vertical direction.

By adopting the technical scheme, compared with the prior art, the invention has the following beneficial effects.

(1) The heat transfer structure of the invention is provided with a notch, so that the position of the detection device is avoided. The sealing material ageing, deformation or failure easily caused by high temperature are avoided, the damage of the sealing structure caused by high temperature is prevented, the replacement maintenance frequency and cost are reduced, the leakage of the heat transfer medium can be effectively prevented, the risks of accidents and disasters are reduced, and the safety of a workplace is improved. The detection device is placed on the outer wall of the culture device in the notch of the heat transfer structure, so that the device can be maintained and operated more conveniently, compared with the device which directly passes through the heat transfer structure, the detection device only needs to be operated and adjusted at the notch, the process of disassembling and reinstalling the heat transfer structure is avoided, and the workload and time consumption are reduced. And the temperature control in the tank is not accurate due to the fact that heat transmission at the sealing position is different from that at other positions, and the growth speed of plant tissues is influenced.

(2) The invention is sleeved on the culture device through the heat transfer structure, realizes accurate control of the temperature in the culture device, provides a proper growth environment for plant tissues, is beneficial to nutrient absorption and growth and development of the plant tissues, and increases the growth rate of the plant tissues.

The following describes the embodiments of the present invention in further detail with reference to the accompanying drawings.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention. It is evident that the drawings in the following description are only examples, from which other drawings can be obtained by a person skilled in the art without the inventive effort. In the drawings:

FIG. 1 is a schematic illustration of a plant tissue bioreactor apparatus of the present invention;

FIG. 2 is a schematic view of a heat transfer structure of the present invention;

FIG. 3 is a schematic diagram of a tank circuit of the present invention;

FIG. 4 is an external overall schematic of the present invention;

FIG. 5 is a schematic diagram of a culture tank cutting structure and an air intake structure;

FIG. 6 is a schematic view of a seed tank cutting structure and an air intake structure;

FIG. 7 is a schematic view of one of the angles of the air intake structure of the present invention;

FIG. 8 is a schematic diagram of a cutting structure of the present invention;

FIG. 9 is a schematic view of a cutting portion of the cutting structure of the present invention;

fig. 10 is a schematic view of the temperature regulating system of the present invention.

In the figure: 11. a detection device; 12. a first tank; 13. a second tank; 14. a seed tank; 141. a first discharge port; 142. a first receiving opening; 15. a culture tank; 151. a second discharge port; 152. a second receiving opening; 16. a delivery line; 161. a seed transferring tube; 162. seed returning pipe; 2. a heat transfer structure; 21. a notch; 22. a first annular sleeve; 23. a second annular sleeve; 24. a heat preservation layer; 241. a first port; 242. a second port; 3. an air intake structure; 31. an air guide bracket; 311. a first straight section; 312. a second straight section; 313. a third straight section; 32. a fixing ring; 33. a fixed plate; 34. an air inlet rod; 4. a cutting structure; 41. a spacer bush; 42. a shearing part; 421. a pin shaft; 422. a blade set; 423. a first blade; 424. a second blade; 425. a rotating end; 426. blade ends; 427. closing the side edges; 428. unfolding the side edges; 43. a transmission part; 44. a power section; 45. a guide cover; 451. an inlet; 453. an outlet; 5. a water tank; 51. a water outlet pipeline; 52. a water inlet pipeline; b100, a steam supply main pipe; c200, a chilled water supply main pipe; e200, a hot water supply main pipe; a3, a temperature-regulating water tank; a301, a water containing cavity; a24, a liquid inlet pipe; a25, a liquid outlet pipe.

It should be noted that these drawings and the written description are not intended to limit the scope of the inventive concept in any way, but to illustrate the inventive concept to those skilled in the art by referring to the specific embodiments.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions in the embodiments will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present invention, and the following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.

In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.

Temperature is one of the key factors affecting plant tissue, especially the growth rate of adventitious roots. Suitable temperatures may provide optimal environmental conditions for plant tissue growth, however, too high or too low temperatures may result in abnormal or stagnant growth. Therefore, the growth temperature of the plant tissue is accurately controlled to ensure the growth speed and improve the yield and quality of the plant tissue.

As shown in fig. 1 to 3, the present invention provides a plant tissue bioreactor for cultivating adventitious roots of ginseng, the plant tissue bioreactor comprising a culture device and a heat transfer structure 2. The culture device is internally provided with culture solution, so that the growth of adventitious roots of ginseng can be promoted, the peripheral wall of the culture device is provided with a detection device 11, and the detection device 11 comprises a plurality of detection units and sampling units and is used for detecting parameters and/or sampling of the culture solution in the culture device. The heat transfer structure 2 is a jacket sleeved on the peripheral wall of the culture device and used for controlling the temperature of the culture device. The heat transfer structure 2 has a notch 21, and the detection device 11 is provided on the outer peripheral wall of the culture device in the notch 21.

The detecting device 11 is mounted on the outer peripheral wall of the culture device from the outside through the notch 21 and protrudes into the inside of the culture device.

The invention controls the temperature in the culture device by sleeving the heat transfer structure 2 on the culture device. The heat transfer structure 2 can perform thermal isolation, is favorable for stabilizing the temperature in the culture device, can effectively prevent the external temperature from generating unnecessary influence on the culture process, keeps the temperature stability, provides the proper growth environment temperature of the adventitious roots of the ginseng, improves the yield and the production efficiency, accelerates the culture process and shortens the production period.

In the invention, the heat transfer structure 2 is provided with the notch 21, the outer wall of the culture device is directly and naked to leak in the notch 21, the detection device 11 is directly arranged on the outer wall of the culture device, naked to leak in the notch 21, so that the detection device 11 is convenient to install, operate and adjust, compared with the process of directly penetrating through the heat transfer structure 2, only the operation and adjustment are needed at the notch 21, the process of detaching and reinstalling the heat transfer structure 2 is avoided, and the workload and time consumption are reduced; because the detection device 11 and the heat transfer structure 2 do not need to be sealed, the sealing material which is easy to cause at high temperature is prevented from aging, deforming or failing, the sealing structure is prevented from being damaged at high temperature, the replacement maintenance frequency and the cost are reduced, the heat transfer medium is also effectively prevented from leaking, the risks of accidents and disasters are reduced, and the safety of a workplace is improved. The lower edge of the heat transfer structure 2 is provided with a notch 21 which is internally and externally communicated, so that the heat transfer structure 2 can be conveniently installed on a culture device.

The notches 21 penetrate the inner and outer sides of the heat transfer structure 2. The outer wall of the culture device is exposed in the notch 21 and is in direct contact with the external atmospheric environment. The heat transfer structure 2 with the indentations 21 has a cavity inside. The heat transfer structure 2 is attached to the outer peripheral wall of the culture apparatus.

The detection device 11 is at a distance from the edge of the notch 21. The heat transfer structure 2 has a certain thickness, and the inner and outer parts of the notch 21 penetrate through the wall thickness of the heat transfer structure 2. The heat transfer structure 2 has a blocking wall for blocking the inner peripheral wall of the notch 21 to form the cavity.

Preferably, the heat transfer structure 2 is sleeved in the middle of the culture device.

The upper end and the lower end of the culture device are provided with a feeding device and a discharging device which are used for adding nutrient components into the culture device or extracting culture solution. The heat transfer structure 2 is sleeved in the middle of the culture device, the notch 21 is arranged at the lower end of the jacket, so that the positions of the feeding device and the discharging device on the culture device can be avoided, the mounting complexity of the heat transfer structure 2 is avoided, and the resource waste is avoided. The jacket can uniformly distribute heat on the periphery of the culture device, so that the culture solution or the sample is uniformly heated.

The culture device is provided with a first tank body 12 and a second tank body 13 which are communicated, the second tank body 13 is arranged to extend downwards from the lower end of the first tank body 12, and the inner diameter of the second tank body is gradually reduced from the inner diameter of the first tank body 12. The heat transfer structure 2 comprises a first annular sleeve 22 and a second annular sleeve 23 which are communicated, the peripheries of the connecting ends of the first tank body 12 and the second tank body 13 are correspondingly sleeved, and the notch 21 extends upwards from the edge of the lower end of the second annular sleeve 23.

The heat transfer structure 2 is sleeved on the periphery of the connecting end of the first tank 12 and the second tank 13 of the culture device and is used for heating the middle part of the culture device. Heat is transferred to the culture fluid within the culture device through the outer wall of the culture device.

Preferably, in the present invention, the upper half of the culture apparatus is a first tank 12 having a cylindrical-like shape and divided into a first part and a second part. The first part is in a round cover shape, the uppermost part is provided with a vertex, and the inner diameter gradually increases from the vertex to the bottom. The second part is a cylinder with two open ends, the upper end is connected with the first part, extends downwards for a certain distance, and the lower end is connected with the second tank 13. The second tank 13 is in an inverted cone shape, and the upper end is connected with the second part of the first tank 12. The bottom surface of the first tank 12 and the top surface of the second tank 13 are open surfaces, and the first tank 12 and the second tank 13 are communicated with each other.

The notch 21 may extend upwardly from the lower edge of the second annular sleeve 23 to the second annular sleeve 23 or may extend to the first annular sleeve 22. The extended arrangement of the notch 21 ensures that sufficient installation space is reserved for the detection unit and sampling unit of the detection device 11.

The notch 21 extends circumferentially a certain distance, and a plurality of detection units are arranged in the notch 21 at intervals transversely and have a certain distance from the inner edge of the notch 21.

The culture device comprises a seed tank 14, the seed tank 14 being used for cultivating adventitious roots. The heat transfer structure 2 is sleeved on the seed tank 14. The first annular sleeve 22 and the second annular sleeve 23 of the heat transfer structure 2 are correspondingly sleeved on the periphery of the connecting end of the first tank body 12 and the second tank body 13 of the seed tank 14, and the notch 21 of the heat transfer structure 2 extends upwards from the edge of the lower end of the second annular sleeve 23 to the first annular sleeve 22.

The first annular sleeve 22 of the seed tank 14 extends from the upper portion of the first tank body 12 of the seed tank 14 to the edge where the first tank body 12 is connected to the second tank body 13. The second annular sleeve 23 of the seed tank 14 extends from the lower end of the first annular sleeve 22 to slightly exceed the connecting edge of the second tank 13 and the first tank 12, and the thickness of the second annular sleeve 23 of the seed tank 14 gradually increases from top to bottom. The notch 21 of the heat transfer structure 2 on the seed tank 14 extends upwards from the edge of the lower end of the second annular sleeve 23 on the seed tank 14 to more than half the axial extension of the first annular sleeve 22.

The second annular sleeve 23 of the seed tank 14 has a wall thickness greater than the wall thickness of the first annular sleeve 22.

The culture device further comprises a culture tank 15. The culture tank 15 is used for culturing adventitious roots cultured in the seed tank 14 again, the heat transfer structure is sleeved on the culture tank 15, a first annular sleeve 22 and a second annular sleeve 23 of the heat transfer structure 2 are correspondingly sleeved on the periphery of the connecting ends of the first tank body 12 and the second tank body 13 of the culture tank 15, and a notch 21 of the heat transfer structure 2 extends upwards from the edge of the lower end of the second annular sleeve 23 to the second annular sleeve 23.

The first annular sleeve 22 of the culture tank 15 extends from the upper part of the first tank body 12 of the culture tank 15 to the edge where the first tank body 12 is connected with the second tank body 13; the second annular sleeve 23 of the culture tank 15 extends from the lower end of the first annular sleeve 22 down the second tank 13 a distance of more than half the second tank 13; the notch 21 of the heat transfer structure 2 on the culture tank 15 extends upwards from the edge of the lower end of the second annular sleeve 23 on the culture tank 15 to be more than half the axial extension length of the second annular sleeve 23.

The volume of the culture tank 15 is much larger than the volume of the seed tank 14. Specifically, the maximum inner diameter of the culture tank 15 is much larger than the maximum inner diameter of the seed tank 14. The volume of the seed tank 14 was 200L, and the volume of the culture tank 15 was 1000L and 1500L. The indentations 21 of the seed tank 14 and the culture tank 15 extend approximately the same or the same length in the lateral direction and the vertical direction.

In the invention, a first discharge port 141 is arranged at the bottom of the seed tank 14, and a first receiving port 142 is arranged on the side wall. The bottom of the culture tank 15 is provided with a second discharge port 151, and the side wall is provided with a second receiving port 152. The culture device further comprises a transfer line 16. The transfer line 16 includes a seed transfer pipe 161 and a seed return pipe 162, the seed transfer pipe 161 for transferring the culture liquid in the seed tank 14 into the culture tank 15. The seed returning pipe 162 is used for transferring the culture solution in the culture tank 15 to the seed guiding tank 14. The seed transfer pipe 161 is connected between the first discharge port 141 of the seed tank 14 and the second receiving port 152 of the culture tank 15. The seed returning pipe 162 is provided between the first receiving port 142 of the seed tank 14 and the second discharging port 151 of the culture tank 15. The culture medium in the seed tank 14 and the culture tank 15 have different compositions, and adventitious roots grow in the seed tank 14 first, and after growing to a certain stage, they are transferred to the culture tank 15 through the seed transfer tube 161, and then grow in the next stage. Adventitious roots are grown in the culture tank 15 for a certain period of time and then transferred back to the seed tank 14 through the seed returning pipe 162 for cultivation.

The seed shifter 161 is provided with a five-way valve arranged at the lower end of the seed shifter 161, a seed shifter valve arranged at the upper end of the seed shifter 161 and close to the culture tank 15, a seed tank bottom valve arranged at the bottom of the seed tank 14, a CIP inlet pipe connected with the seed shifter 161 and a CIP valve arranged on the CIP inlet pipe, and a seed shifter high-temperature steam inlet valve arranged on a seed shifter high-temperature steam branch inlet pipe connected with the seed shifter 161;

the seed returning pipe 162 is characterized in that one end of the seed returning pipe 162 is connected with the bottom of the culture tank 15, the other end of the seed returning pipe 162 extends upwards and is connected with the side wall of the upper portion of the seed tank 14, the seed returning pipe 162 is provided with a five-way valve arranged at the lower end of the seed returning pipe 162, the seed returning valve arranged at the upper end of the seed returning pipe 162 and close to the seed tank 14 is arranged at the bottom valve of the culture tank arranged at the bottom of the culture tank 15, the CIP inlet pipe connected to the seed returning pipe 162 and the CIP valve arranged on the CIP inlet pipe are arranged on a seed returning pipe high-temperature steam inlet valve connected to the seed returning pipe 162.

When the adventitious roots of ginseng in the pot need to be transferred from the seed pot 14 to the culture pot 15, the seed transfer pipe 161 needs to be disinfected firstly, a seed transfer pipe high-temperature steam inlet valve arranged on a high-temperature steam branch air inlet pipe of the seed transfer pipe is opened, high-temperature steam is introduced into the seed transfer pipe 161 to disinfect the interior of the seed transfer pipe 161, and when the high-temperature steam is introduced to disinfect the seed transfer pipe 161, a seed pot bottom valve connected with the lower end of the seed transfer pipe 161 is also introduced with the high-temperature steam to disinfect;

After the seed transfer is completed, the culture tank 15 may pass through the seed returning tube 162 to the adventitious root of the ginseng after the reflux portion of the seed tank 14 is diluted, the seed returning tube 162 needs to be sterilized by high temperature steam before the reflux, the seed returning tube high temperature steam inlet valve arranged on the high temperature steam branch air inlet tube of the seed returning tube is opened to introduce high temperature steam into the seed returning tube 162 to sterilize the interior of the seed transferring tube 161, and when the high temperature steam is introduced to sterilize the seed returning tube, the bottom valve of the culture tank connected to the lower end of the seed returning tube 162 is also sterilized by high temperature steam.

The five-way valves are arranged on the seed transfer pipe 161 and the seed return pipe 162 and are identical in position, the five-way valves on the seed transfer pipe 161 are provided with five interfaces, two interfaces of the five-way valves on the seed transfer pipe 161 are respectively connected with the seed transfer pipe 161, and the other three interfaces are respectively connected with a discharge valve, a drain valve and a CIP discharge valve which are connected with the seed transfer pipe 161;

two interfaces of the five-way valve on the seed returning pipe 162 are respectively connected with the seed returning pipe 162, and the other three interfaces are respectively connected with the discharge valve, the blow-down valve and the CIP discharge valve which are connected with the seed returning pipe 162.

As shown in fig. 10, a temperature control system is provided in one embodiment of the present invention, and the temperature control system includes a supply unit, a heat transfer structure 2 coated on an outer surface of the culture apparatus, and a transfer unit communicating the supply unit with the heat transfer structure 2. Wherein the supply unit carries a plurality of liquids of different temperatures and leads in and out the heat transfer structure 2 via the transport unit. The supply unit comprises several parallel arrays, each for introducing and removing a different substance into and from the culture device or the heat transfer structure 2, respectively.

In particular, the tempering system further comprises a tempering tank A3 communicating with the supply unit. The temperature-adjusting water tank A3 is connected to the supply unit in parallel with the culture apparatus. The supply unit is capable of carrying a plurality of liquids having different temperatures and introducing and discharging the liquids into and from the temperature-adjusting water tank A3 via the conveying unit A2. The water in the temperature-adjusting water tank A3 circulates to the heat transfer structure 2 to exchange heat with the culture device and then flows back to the temperature-adjusting water tank A3.

In this embodiment, the temperature adjusting water tank A3 is additionally arranged in the temperature adjusting system, and the temperature adjusting water tank A3 and the heat transfer structure 2 are both connected to the supply unit, so that the temperature adjusting water tank A3 and the heat transfer structure 2 form a loop connected in parallel with the supply unit, the temperature adjusting water tank A3 and the heat transfer structure 2 can circulate heat exchange medium with the supply unit at the same time, and the temperature adjusting water tank A3 and the heat transfer structure 2 can be connected in series by the supply unit to form a temperature adjusting loop, so that the temperature adjusting water tank A3 circulates with the heat transfer structure 2 directly, the temperature adjusting loop can adjust the temperature of the culture device independently when a supply source of the temperature adjusting system is damaged and maintained, and the fault tolerance of the temperature adjusting system is improved.

In an embodiment of the present invention, a temperature regulating system for a culture apparatus is presented. The temperature regulating system is additionally provided with a temperature regulating water tank A3 and a heat exchange structure A23.

Specifically, the temperature-regulating water tank A3 is provided with a water-containing chamber a301 for containing and heating the liquid that can be fed into the heat transfer structure 2. In addition, the heat exchange structure A23 is arranged close to the water containing cavity A301 and can exchange heat with the liquid in the water containing cavity A301, so that a circulation loop capable of supplying heat to the heat transfer structure 2 is formed between the temperature-adjusting water tank A3 and the heat transfer structure 2.

Wherein the water containing cavity A301 and the heat transfer structure 2 are connected with the supply unit through the conveying unit to form a circulation loop. The conveying unit comprises a water inlet pipe and a water outlet pipe which are communicated with the supply unit and the water containing cavity A301, and the temperature of the culture device is maintained by the temperature regulating water tank A3.

Further, the supply unit includes a hot water supply main E200, a chilled water supply main C200, and a steam supply main B100. Each of which carries hot water, chilled water and high temperature steam.

The conveying unit A2 comprises a liquid inlet pipe A24 and a liquid outlet pipe A25 which are communicated with the temperature-regulating water tank A3 and the supply unit. The liquid inlet pipe A24 is provided with a plurality of branches, and can send chilled water and high-temperature steam into the temperature-regulating water tank A3 or the heat exchange structure A23.

The temperature regulating system further comprises a master control unit. The total control unit is electrically connected with the pneumatic valves connected in series in each branch of the liquid inlet pipe A24, and can adjust the flow ratio of the chilled water and the high-temperature steam to the temperature-adjusting water tank A3 and the heat exchange structure A23. Chilled water and high-temperature steam enter the temperature-adjusting water tank A3 or the heat exchange structure A23 and can exchange heat with hot water in the water containing cavity A301, so that the hot water is ensured to meet the requirements of the plant tissue culture device when being conveyed into the heat transfer structure 2. Even when the supply unit cannot directly supply hot water to the heat transfer structure 2, the temperature-adjusting water tank A3 can be used to supply hot water to the heat transfer structure 2, and the operation of the culture apparatus can be maintained.

In another embodiment, an electric heating structure may be disposed in the temperature-adjusting water tank A3 to directly heat the liquid in the water containing cavity a 301. Thus, when the hot water cannot be directly supplied from the hot water supply main pipe E200 to the heat transfer structure 2, the hot water supply main pipe E200 and the hot water recovery main pipe can be controlled to directly communicate the heat transfer structure 2 with the temperature-adjusting water tank A3. In this way, a single circulation loop is formed between the temperature-regulating water tank A3 and the heat transfer structure 2, and the temperature-regulating water tank A3 can be utilized to directly convey hot water to the heat transfer structure 2, so that the requirement of the culture device on the temperature of the hot water is ensured.

In particular, during normal operation of the supply unit, the heat transfer structure 2 may be fed with hot water directly supplied from the hot water supply main E200 or with hot water fed from the temperature-regulating water tank A3. At this time, the temperature of the hot water in the water containing chamber a301 can be further raised and then transferred into the hot water supply main E200, so that the temperature compensation effect can be achieved on the hot water in the hot water supply main E200, and the temperature of the culture device can be more finely adjusted and controlled.

The biological reaction device of plant tissues adopts the combination of the seed tank 14 and the culture tank 15, can improve the quality of adventitious roots, and can provide a proper growth environment, including control of factors such as temperature, humidity, nutrient components and the like. By adjusting the environmental factors, the growth and development of the adventitious roots can be promoted, the growth of the adventitious roots is protected, convenient operation is provided, and the production efficiency and the product quality are improved.

The detection device 11 is avoided in the position of the notch 21 on the heat transfer structure 2, the detection device 11 can be prevented from being influenced by the heat transfer structure 2 on the premise of not influencing the detection result, and the service life of the device is prolonged.

Although the notch 21 is provided on the heat transfer structure 2, the detection device 11 and the heat transfer structure 2 can be conveniently installed and can avoid equipment damage or heat transfer medium leakage caused by the loose seal, but the problems of incomplete coverage of the heat transfer structure 2 and uneven temperature of the culture solution in the culture device can be caused.

In order to solve the above technical problems, as shown in fig. 4 to 7, an air intake structure 3 is provided in the culture apparatus. The air inlet structure 3 can feed sterile air into the device to drive the culture solution in the device to flow, so that the temperature in the culture solution is kept balanced, and the growth of adventitious roots is promoted.

The second tank body 13 at the bottom of the seed tank 14 is provided with at least two groups of air inlet structures 3 for supplying air into the tank and is positioned below the heat transfer structure 2 of the seed tank 14; the air inlet structure 3 of the seed tank 14 is oppositely arranged on the peripheral wall of the second tank body 13 of the seed tank 14. The second tank body 13 at the bottom of the culture tank 15 is provided with at least four groups of air inlet structures 3 for supplying air into the tank and is positioned below the heat transfer structure 2 of the culture tank 15; the air inlet structure 3 of the culture tank 15 is circumferentially and uniformly distributed on the outer peripheral wall of the second tank body 13 of the culture tank 15.

The air inlet structure 3 is detachably connected to the side walls of the second tank body 13 of the seed tank 14 and the culture tank 15.

The air inlet structure 3 comprises an air guide bracket 31, and the air guide bracket 31 is a bent pipeline and comprises a first straight section 311, a second straight section 312 and a third straight section 313. The first straight section 311 is perpendicular to the second straight section 312, the second straight section 312 and the third straight section 313 are arranged at an obtuse angle, and the first straight section 311 and the second straight section 312 and the third straight section 313 are in smooth transition.

One end of the first straight section 311 is provided with a bell mouth, the air inlet structure 3 is also provided with a fixed disk 33 and a fixed ring 32, the outer diameter of the fixed ring 32 is larger than that of the fixed disk 33, and the thickness is 2-5cm. The first straight section 311 of the air guide bracket 31 is detachably mounted on the tank bottom side wall of the second tank body 13 under the interaction of the fixed disk 33 and the fixed ring 32. The third straight section 313 is provided with an air inlet rod 34 in a sleeved mode, the inside of the rod body of the air inlet rod 34 is hollow, and the surface of the air inlet rod is made of a microporous material formed by titanium sintering. The gas enters the gas guide bracket 31 from the first straight section 311 of the gas guide bracket 31 of the gas inlet structure 3, and sequentially passes through the second straight section 312 and the third straight section 313 to the gas inlet rod 34.

The horn-shaped end of the first straight section 311 is firstly contacted with air to be fed into the tank, so that the air can be guided into the air inlet structure 3, and the air sequentially passes through the first straight section 311, the second straight section 312 and the third straight section 313 to enter the air inlet rod 34, and the micropores distributed on the surface of the air inlet rod 34 enter the tank.

In the invention, two groups of air inlet structures 3 and two groups of cutting structures 4 are arranged in the seed tank 14, the cutting structures 4 are also arranged below the gaps 21 of the heat transfer structure 2, and the air inlet structures 3 and the cutting structures 4 are arranged at intervals, namely, the air inlet structures 3 and the cutting structures 4 are arranged adjacently. The two groups of air inlet rods 34 are arranged on the same plane and are parallel to each other during installation, the air inlet rods 34 have a certain length, the air inlet rods 34 are arranged in a mode that the middle part of the length of the air inlet rods 34 is connected with the center of the side wall of the tank bottom of the second tank body 13 to form a middle shaft surface and are arranged on two sides of the middle shaft surface, and preset intervals are arranged between the air inlet rods 34 and the cutting structure 4 in the direction that the center of the second tank body 13 extends to the periphery. The air inlet rod 34 adopts the installation mode, so that the air inlet is fully and uniformly distributed. The air intake bars 34 extend from the third straight section 313 to more than half the distance between every two air intake structures 3.

The four groups of air inlet structures 3 of the culture tank 15 extend into the second tank body 13. The air inlet structure 3 is provided with an air inlet rod 34 which is kept horizontal and has a certain length, and the air inlet rod 34 conveys sterile air into the second tank body 13 of the culture tank 15 through bubbles; the projections of any two opposite sets of air intake bars 34 in the tank are approximately parallel, and the projections of two adjacent sets of air intake bars 34 in the tank are approximately perpendicular. The four groups of air inlet structures 3 of the culture tank 15 extend obliquely upwards into the second tank body 13; one group of air inlet structures 3 of the culture tank 15 is arranged right below the notch 21, and the air inlet structures 3 are circumferentially arranged on the second tank body 13 of the culture tank 15 at intervals.

In the invention, four groups of air inlet structures 3 and two groups of cutting structures 4 are arranged in a culture tank 15 and are positioned at different heights in the tank bottom side wall of a second tank body 13, one group of cutting structures 4 are arranged at intervals in each two groups of air inlet structures 3, the connection line between the installation position of one group of cutting structures 4 on the same side of the tank bottom side wall of the second tank body 13 and the installation position of two adjacent groups of air inlet structures 3 in the tank body approximately forms an isosceles triangle, and the installation position of the cutting structures 4 is higher than the air inlet structures 3 in the vertical direction. The four groups of air inlet structures 3 are uniformly distributed in the side wall of the tank bottom of the second tank body 13, and the four groups of air inlet structures 3 are arranged at equal intervals. The air intake bars 34 of the air intake structure 3 are kept horizontal and have a certain length. The projections of any two opposite sets of air intake bars 34 in the tank are approximately parallel, and the projections of two adjacent sets of air intake bars 34 in the second tank 13 are approximately perpendicular.

The air inlet structure 3 is arranged at an intermediate position between the jacket and the bottom of the second tank 13. Avoid because air inlet structure 3 sets up lowerly, to the inhomogeneous or work that influences first discharge gate 141 or second discharge gate 151 of culture solution stirring of culture apparatus inside peripheral edge. Too high setting of the air inlet structure 3 is avoided, and the culture solution below the air inlet structure 3 in the culture device cannot be uniformly mixed.

The air inlet structure 3 plays a better stirring role on the adventitious roots and the culture solution in the seed tank 14 and the culture tank 15. Under the stirring action, the culture solution is fully mixed and subjected to heat exchange, so that the temperature of the culture solution is balanced. And the culture solution can provide sufficient sterile air for the adventitious roots to breathe in the tank body, and good sterile air supply can promote the respiratory metabolism of the adventitious roots and the root growth of the adventitious roots.

The cutting structure 4 can cut the adventitious roots growing to a certain number or a certain volume into small sections with the length of 5-15mm, so that the phenomenon that the roots cannot normally stretch to influence the growth and development of plants due to the fact that the adventitious roots are excessively long and are wound in a culture device to form entangled root systems is avoided.

The side walls of the second tank body 13 of the seed tank 14 and the culture tank 15 can be provided with a mounting hole of the air guide bracket 31 of the air inlet structure 3 and a mounting hole of the spacer 41 which can pass through the cutting structure 4, and the two mounting holes have different sizes.

Fig. 8 to 9 show the cutting structure 4 on the seed tank 14 and the culture tank 15. The cutting structure 4 is arranged on the side wall of the bottom of the second tank body 13 of the seed tank 14 and the culture tank 15, and is used for cutting adventitious roots growing to a certain quantity or a certain volume to a length suitable for secondary culture of the adventitious roots, and preferably, the adventitious roots are cut into small sections of 5-15 mm.

In the invention, the mounting holes of the cutting structure 4 and the second tank 13 are provided with the spacer 41. The side wall of the bottom of the second tank body 13 is provided with a circular mounting hole, and the outer peripheral surface of the spacer 41 is cylindrical and has the same diameter as the mounting hole. The spacer 41 is detachably mounted in a mounting hole in the side wall of the second tank 13. One side of the spacer 41 is connected with the shearing part 42, and the other side is connected with the power part 44, so that the shearing part 42 and the power part 44 are separated from the inside and the outside of the second tank 13. The shearing part 42 is installed at a side of the spacer 41 facing the inside of the second tank 13, and the power part 44 is installed at a side of the spacer 41 facing the outside of the second tank 13. The power part 44 can drive the shearing part 42 to cut the adventitious roots, and the cross section of the shearing part 42 is smaller than that of the spacer 41, so that the cutting structure 4 can be simply and quickly arranged on the second tank 13.

In one embodiment of the present invention, the cutting portion 42 of the cutting structure 4, the cutting portion 42 includes a pin 421, a first blade 423 and a second blade 424 attached to the first blade 423. The first blade 423 and the second blade 424 are connected in series by a pin 421. The pin 421 is perpendicular to the plane of the first blade 423 and the second blade 424.

In the connection mode: the first blade 423 is fixed to the pin 421. The second blade 424 is rotatable about the pin 421 with respect to the first blade 423. The opposite sides of the two blades form a continuously opened and closed notch which can be used for shearing adventitious roots.

Specifically, as the second blade 424 rotates, the edges of the two blades move from a spaced condition to an overlapping condition, forming open and close cuts in the opposite sides of the two blades.

In this embodiment, the shearing portion 42 includes two blades that can rotate relatively and the sides of the two blades are attached to each other, so that the edges of the two blades that are overlapped and staggered are formed with continuously open and shut notches, and the adventitious root can be completely cut off by extruding the adventitious root through the notches, so that the problem that the adventitious root is not cut off and even the shearing portion 42 is wound to cause damage to the cutting structure 4 is avoided, the smoothness of the adventitious root notch can be ensured, necrosis after the adventitious root is cut is avoided, and the growth speed and quality of the adventitious root are improved.

In one embodiment of the present invention, a shear 42 is described that is capable of cutting out adventitious roots of the same length.

The shearing portion 42 includes a plurality of blade sets 422 connected in series to the pin 421. The plurality of blade groups 422 are equally spaced apart along the axial direction of the pin shaft 421 to form a multi-layered structure. Each blade set 422 is formed by two-by-two matching of the first blade 423 and the second blade 424.

Specifically, all the first blades 423 in the blade set 422 are parallel to each other and fixed with the rotating shaft, all the second blades 424 are parallel to each other and are connected at the ends of the second blades 424 in the length direction of the second blades themselves to form a frame body coated on the outer side of the first blades 423, and the second blades 424 rotate relative to the first blades 423 to form a continuously opened and closed incision.

Thus, when the second blade 424 is driven to rotate relative to the first blade 423, the adjacent blade group 422 simultaneously shears the adventitious roots entering the shearing portion 42 so that the length of the cut adventitious roots coincides with the interval between the adjacent blade groups 422.

In this embodiment, the shearing part 42 is provided with a plurality of groups of blades, and the blade groups 422 are arranged at fixed intervals, so that adventitious roots with consistent length can be obtained by cutting adventitious roots, the adventitious roots in the pot have the same growth state, the nutrient content of nutrient solution can be adjusted regularly, the adventitious roots can be trimmed conveniently, the circulation of nutrient solution in the pot is promoted, stable growth conditions are maintained, and the cultivation efficiency and the quality of the adventitious roots are improved.

In another embodiment of the present invention, a shear 42 is described having two blade sets 422.

The two first blades 423 are sleeved on the pin shaft 421 at intervals and are arranged in parallel with each other. The two second blades 424 respectively abut the outer sides of the first blade 423 facing away from the space.

The two second blades 424 are disposed in parallel and are coupled at their ends in the longitudinal direction thereof to form a frame structure. The second blade 424 may be fitted inside the first blade 423, or may be covered from outside the first blade 423.

Preferably, the second blade 424 is coated from the outside of the first blade 423, i.e. is attached to the side of the first blade 423 facing away from the space.

In this embodiment, the shearing portion 42 is provided with two blade groups 422, and the second blades 424 are combined into a whole, which can be connected from any side of the shearing portion 42 and drive the two second blades 424 to rotate at the same time, so that the structure of the shearing portion 42 is simplified, the shearing portion 42 can cut out adventitious roots with the same length, and the synchronous opening and closing of the incisions of the two blade groups 422 can be maintained, so that the efficiency of trimming adventitious roots is improved.

In another embodiment of the present invention, a second blade 424 of the shear 42 is described. The second blade 424 includes a rotating end 425 and a blade end 426.

One end of the blade end 426 is connected to the rotating end 425, and the other end extends in the diameter direction of the rotating end 425 and is suspended at the outer circumference to form a cantilever shape.

The rotating end 425 is sleeved on the pin shaft 421, and when the second blade 424 rotates relative to the first blade 423, the blade end 426 can rotate around the pin shaft 421 to form a continuously opened and closed notch.

In another embodiment of the present invention, a shear 42 is described that forms a curved cut. The first blade 423 and the second blade 424 have the same structure and shape. I.e., both the rotating end 425 and the blade end 426.

Wherein the blade end 426 includes a closed side 427 for cutting and an open side 428 opposite the closed side 427. The closure profiles 427 and the deployment profiles 428 extend radially from the rotational end 425 to the other end and gradually converge toward each other.

In another embodiment of the present invention, a shear 42 capable of collecting adventitious roots is described. The two ends of the closed side 427 are located on the same diameter line. And, the closed side 427 extends in an arc from the rotating end 425 to the other end. Finally, the closed side 427 of the blade end 426 is formed with an inwardly concave recess.

The slit is gradually closed from both ends of the closing side 427 toward the notch when the second blade 424 rotates relative to the first blade 423.

Further, the second blade 424 includes a plurality of the blade ends 426 evenly distributed along the outer circumference of the rotating end 425. For example, three blade ends 426 are provided at 120 ° intervals on the outer circumference of the rotating end 425. Four blade ends 426 are disposed at intervals of degrees at the outer circumference of the rotating end 425.

Preferably, the second blade 424 is provided with two blade ends 426 symmetrically distributed about the rotational end 425.

In particular, the deployment side 428 extends along an arc from the rotational end 425 to the outer periphery, and the closure side 427 also extends along an arc from the rotational end 425 to the outer periphery. And, the open side 428 and the closed side 427 gradually converge at a point. The second blade 424 has an S-shape and is formed of two blade ends 426 connected to a rotating end 425 and disposed at 180 ° intervals.

In another embodiment of the present invention, a shear 42 is described that promotes the circulation of adventitious roots. The blade ends 426 are curved upwardly in a circumferential direction from the closed side 427 to the open side 428.

Preferably, the blade end 426 is configured as a spiral surface along the diameter of the rotating end 425.

In another embodiment, the first blade 423 is provided in a long strip shape for better guiding the circulation of the adventitious roots in the tank. And the first blade 423 is provided at both ends with sharp corners inclined to the diameter thereof. The side of the sharp corner opposite the closed side 427 is concave toward the first blade 423.

In another embodiment of the invention, a cutting structure 4 for use in a can is presented.

The cutting structure 4 is arranged in the inverted cone, i.e. the spacer 41 is arranged at the bottom of the can,

in this embodiment, the cutting structure 4 is arranged at the bottom of the tank, so that the adventitious roots in the tank can be cut quickly, the adventitious roots in the tank are smooth in cut, have the same growth state, facilitate the periodical pruning of the adventitious roots in the tank, promote the circulation of nutrient solution in the tank, maintain good growth conditions of the adventitious roots, and improve the efficiency and quality of the cultivation of the adventitious roots in the tank.

In another embodiment of the invention, a number of in-can cutting structures 4 are described. The cutting structure 4 is detachably connected at the tank wall inside the tank.

Specifically, the cutting structure 4 includes a cutting portion 42 and a power portion 44, and the power portion 44 can drive the cutting portion 42 to cut adventitious roots. The power portion 44 is not directly connected with the shearing portion 42, the shearing portion 42 is disposed in a tank cavity in the tank, and the power portion 44 is disposed outside the tank. The power section 44 and the shear section 42 are spaced apart by a tank wall within the tank.

In particular, a spacer 41 is also provided in the tank. The tank wall in the tank is provided with a circular mounting hole, and the outer peripheral surface of the spacer 41 is cylindrical and has the same diameter as the mounting hole. The spacer 41 is removably inserted into a mounting hole in the tank wall.

One side of the spacer 41 is connected with the shearing part 42, and the other side is connected with the power part 44, so that the shearing part 42 and the power part 44 are separated from each other in the tank. The shearing part 42 is installed on one side of the spacer 41 facing the cavity of the can body, and the power part 44 is installed on one side of the spacer 41 facing the inside and outside of the can body. The spacer 41 can be inserted directly into the cutout 42 from the mounting hole by making the cross section larger than the cutout 42, so that the cutting structure 4 can be simply and quickly disposed in the can.

Spacer 41 has a variety of patterns and in some embodiments of the invention spacer 41 is annular and formed with an annular groove. When the spacer 41 is annular, the middle part of the spacer 41 is a thin-wall plate, and an annular groove formed by sinking towards one side of the plate surface is arranged on the outer circumference of the thin-wall plate.

In other embodiments, spacer 41 is cylindrical and formed with a cylindrical recess.

The cavity opening of the spacer 41 is always located at the end face of the spacer 41. In some embodiments, the spacer 41 is integral with the tank wall and is formed by the tank wall being recessed into the cavity of the tank. At this time, the groove opening of the spacer 41 is formed on the surface of the can.

In other embodiments, the spacer 41 is a separate piece that is separable from the tank wall. The spacer 41 may be removably attached to the tank wall by fasteners or may be secured to the tank wall by adhesive or welding.

In this embodiment, be provided with in the jar and cut out structure 4 that can tailor the adventitious root, still set up spacer 41 between shearing portion 42 and power portion 44 simultaneously for cut out structure 4 dispersedly install in jar wall in the jar, outside, thereby both can cut structure 4 control the length of adventitious root in order to maintain and promote the cultivation efficiency of adventitious root, can also avoid microorganism or impurity to get into in the jar through cut structure 4, improved the leakproofness in the jar.

In another embodiment of the invention, a spacer 41 of a cutting structure 4 is described. The spacer 41 can make the power part 44 better attract from one side of the spacer 41 and drive the shearing part 42 to perform cutting action, so that the structure of mutual attraction between the shearing part 42 and the power part 44 is simplified, and the attraction of the power part 44 to the shearing part 42 is increased.

Specifically, the spacer 41 protrudes from the surface of the tank wall toward the tank cavity along the vertical direction of the tank wall. The spacer 41 forms a groove on the other side, corresponding to the position where the spacer 41 protrudes toward the cavity of the can body, as seen from the outside of the can inner wall.

In particular, the shearing part 42 is connected with the outer convex surface of the spacer 41, and the power part 44 is installed at the groove. Preferably, the shearing part 42 is provided with a member which is sleeved on the periphery of the spacer 41 and can rotate relative to the spacer 41, and the power part 44 attracts and drives the member from the other side of the spacer 41 through the spacer 41, so as to drive the shearing part 42 to cut the adventitious roots.

In this embodiment, by optimizing the shape of the spacer 41, the spacer 41 is protruded into the cavity of the tank body, so that the attraction and the driving effect of the power portion 44 on the shearing portion 42 can be enhanced, the cutting structure 4 is ensured to better shear the adventitious roots, the adventitious roots are prevented from winding and agglomerating, and the faster growth of the adventitious roots is promoted.

In another embodiment of the invention, a spacer 41 of another cutting structure 4 is described. Unlike the spacer 41 in the previous embodiment, the outer circumference of the spacer 41 is provided with an annular groove recessed in the direction of the central axis thereof, and the middle portion of the spacer 41 is located between the groove bottom and the groove top in the direction of the central axis of the annular groove.

Preferably, the middle of the spacer 41 is flush with the groove top, i.e. the middle of the spacer 41 is flush with the surface of the tank wall inside the tank.

Specifically, when the spacer 41 is disposed on the can, the annular groove of the spacer 41 extends from the surface of the can wall toward the can body cavity along the direction of the perpendicular to the can wall, and the notch of the annular groove is located on the surface of the can wall in the can, so that the spacer 41 forms an annular structure having the annular groove.

Preferably, the middle part of the spacer 41 protrudes towards the cavity of the tank body to form a cylindrical structure with a cylindrical groove. In this embodiment, by optimizing the shape of the spacer 41, the spacer 41 is protruded into the cavity of the tank body, so that the attraction and the driving effect of the power portion 44 on the shearing portion 42 can be enhanced, the cutting structure 4 is ensured to better shear the adventitious roots, the adventitious roots are prevented from winding and agglomerating, and the faster growth of the adventitious roots is promoted.

In another embodiment of the invention, a spacer 41 is described that is removably mounted within a canister. The tank wall is provided with a mounting hole, and if the spacer bush 41 is annular, the annular groove of the spacer bush is embedded into the cavity of the tank body and is fixed on the mounting hole; if the spacer 41 is barrel-shaped, it is fixed to the mounting hole in such a manner that the bottom of the barrel is fitted into the cavity of the can.

Specifically, a mounting hole is formed in the tank wall in the tank, and the outer peripheral surface of the spacer 41 is formed in a cylindrical shape and has the same diameter as the mounting hole. The spacer 41 is removably inserted into a mounting hole in the tank wall.

In this embodiment, one side of the spacer 41 is connected to the shear part 42, and the other side is connected to the power part 44, so that the shear part 42 and the power part 44 are separated from each other inside and outside the tank. The shear section 42 is mounted within the tank cavity and the motive section 44 is mounted inside and outside the tank. The cutting portion 42 can be directly placed into the can body cavity in the can from the mounting hole, thereby enabling the cutting structure 4 to be simply and quickly disposed on the can.

In another embodiment of the invention, a mounting location of the cup and spacer 41 is described.

The tank is internally provided in a shape of a revolution body, and the central axis thereof is in the vertical direction. The tank wall in the tank comprises at least a portion extending from the top down and gradually sloping towards the central axis in the tank. The portion forms an inverted cone.

Preferably, the tank wall further comprises a portion of a cylinder parallel to the central axis. The cylindrical part is connected with the inverted cone part to form an integral in the tank, and the inverted cone part is arranged below to form a tank bottom in the tank. The spacer 41 is arranged in the inverted cone, i.e. the spacer 41 is arranged at the bottom of the can, so that the cutting structure 4 is distributed at the bottom of the can.

In the embodiment, the tank bottom with the inverted cone shape is arranged in the tank, so that the pressure of the nutrient solution on the adventitious roots can be reduced, and the growth of the adventitious roots is facilitated. And the bottom of the inverted cone can play a role in converging adventitious roots suspended in nutrient solution, so that the spacer 41 and the cutting structure 4 are arranged on the inverted cone bottom, and the cutting structure 4 is ensured to intensively and efficiently cut the adventitious roots.

In other embodiments of the present invention, a cutout 42 of a cutting structure 4 is described. The cutout 42 includes a first blade 423 and a second blade 424. The first blade 423 and the second blade 424 are disposed adjacent to each other and are connected in series by a rotation axis perpendicular to the adjacent surfaces. The first blade 423 and the second blade 424 can both rotate freely around the rotating shaft, and the opposite sides of the two blades form a continuously opened and closed notch which can be used for shearing adventitious roots.

Alternatively, the first blade 423 is fixedly connected, and the second blade 424 rotates about the rotation axis relative to the first blade 423.

More preferably, the first blade 423 is fixedly connected to the spacer 41, and the second blade 424 is sleeved on the spacer 41 and is rotatable relative to the central axis of the spacer 41. Meanwhile, the power part 44 is provided with a rotor which moves circularly in the groove. The rotor is provided with a magnetic attraction member for attracting the second blade 424 via the spacer 41.

In this embodiment, the shearing portion 42 is formed by two blades that can rotate relatively, and forms a constantly open-close incision by the two blades, and the adventitious root is sheared through the incision to make the adventitious root incision level, so that the dragging and tearing of the adventitious root are avoided, the success rate of the adventitious root shearing is improved, necrosis after the adventitious root is cut is avoided, and the yield and quality of the adventitious root are improved.

In another embodiment of the present invention, a shear 42 is described that is capable of cutting out fixed length adventitious roots.

The shearing portion 42 includes at least two blade groups 422 disposed at intervals in the axial direction. The blade set 422 includes the first blade 423 and the second blade 424 with their sides in contact. The spacing between adjacent blade sets 422 is between 10mm and 15 mm.

In another embodiment of the invention, a cutting structure 4 in a can is presented. The cutting structure 4 is further provided with a transmission portion 43 between the cutting portion 42 and the power portion 44.

Specifically, the transmission part 43 is cylindrical, and is sleeved on the spacer 41. The inner cylinder surface of the transmission part 43 and the outer circumferential surface of the spacer 41 may be slidably connected, or may be rotatably connected and connected together by a bearing. In particular, the transmission portion 43 can rotate about the spacer 41. The transmission part 43 is connected with the second blade 424 and rotates synchronously, and the power part 44 drives the shearing part 42 to cut the adventitious roots through the transmission part 43.

In another embodiment of the present invention, a pod 45 of a cutting structure 4 is described. The cutting structure 4 is arranged in the tank, so that the circulation of the adventitious roots in the cavity of the tank body is better promoted, the repeated cutting of a part of adventitious roots by the cutting structure 4 is avoided, and the guide cover 45 for guiding the liquid to flow is further arranged on the cutting structure 4.

Specifically, the pod 45 is a cylindrical thin-walled tube and is provided with cavities penetrating both ends in the direction of the central axis thereof.

The pod 45 is fitted around the outer periphery of the cutout 42. One end of the pod 45 protrudes from the cutout 42 and has an inlet 451 at the end. The inlet 451 is parallel to the circular surface formed by the rotation of the second blade 424. The other end of the pod 45 extends toward the tank wall and has an outlet 453 at the end face. The outlet 453 is sleeved on the periphery of the transmission part 43, so that the air guide sleeve 45 at least partially covers the transmission part 43.

In this embodiment, the dome 45 is disposed on the outer periphery of the shearing portion 42, so that the circulating flow area of the nutrient solution in the tank is increased, the adventitious roots are promoted to circulate in the cavity of the tank in a large range, the adventitious roots in the area near the shearing portion 42 are prevented from being influenced by the shearing portion 42 to circulate in a small range, and the good circulating state in the tank is ensured, so that the adventitious roots can be trimmed.

At least two groups of cutting structures 4 are arranged on the side walls of the bottom of the second tank body 13 of the seed tank 14 and the culture tank 15, the two groups of cutting structures 4 are symmetrically arranged on two sides of the side wall of the bottom of the second tank body 13 by taking the center of the bottom of the second tank body 13 as the center, and the installation positions of the two groups of cutting structures 4 are equal to the distance between the center of the bottom of the second tank body 13. One end of the cutting structure 4 provided with the power part 44 extends outwards to the outside of the second tank body 13, and one end provided with the cutting part 42 extends towards the central axis direction in the second tank body 13 and is approximately perpendicular to the position formed by the tank bottom side wall of the second tank body 13.

Sterile air enters the second tank body 13 to reduce the density of liquid at the bottom of the second tank body 13, and the air bubbles generated by the air inlet rod 34 disperse adventitious roots at the bottom of the second tank body 13, so that the adventitious roots are ensured not to be hooked with each other and wound. The air inlet structure 3 provides an upward buoyancy force when sterile air is conveyed into the second tank 13, the buoyancy force enables the liquid at the bottom to form liquid flow so that the adventitious roots ascend to the middle part of the culture device, and when the adventitious roots ascend to a certain height, the adventitious roots naturally flow to the bottom of the second tank 13 due to the action of gravity of an object, and the adventitious roots do upward and downward overturning flow in the culture device. The adventitious roots are attracted by attractive force generated by rotation of the shearing part 42 of the cutting structure 4 in the process of vertically tumbling.

When the shearing part 42 in the cutting structure 4 rotates, the blade group 422 in the shearing part 42 rotates rapidly, the liquid flow force generated by the rotation of the blade group 422 can cause the periphery of the cutting structure 4 to form a low air pressure area, high negative pressure is generated around the shearing part 42, and when the adventitious roots are close to the shearing part 42, the adventitious roots are attracted by the shearing part 42 to be sheared.

The installation mode of the air inlet structure 3 and the cutting structure 4 can better trim and maintain the adventitious roots, provide sufficient sterile air for the adventitious roots and better control the length of the adventitious roots, and avoid the overlength and the too dense root systems of the adventitious roots, thereby being unfavorable for discharging and processing.

The air inlet structure 3 is connected with an air filter through an air inlet pipe, a steam branch pipe is connected to the air inlet pipe, and steam is introduced into the air inlet structure 3 through the steam branch pipe. The high-temperature steam is released from the micropores of the air inlet structure 3, floats upwards to the surface of the nutrient solution in the culture tank 15 near the side wall of the conical bottom surface of the culture tank, drives the nutrient solution near the side wall to flow upwards, and simultaneously drives the nutrient solution near the middle part to flow downwards.

The heat transfer structure 2 comprises a heat preservation layer 24, and the heat preservation layer 24 is arranged outside the culture device; the heat preservation layer 24 and the outer wall of the culture device form a cavity; the cavity is provided with a first port 241 and a second port 242 which are communicated with the outside through the heat insulation layer 24 and are respectively used for inputting heat transfer medium into the cavity and discharging the heat transfer medium in the cavity; the first opening 241 is disposed on one end of the insulation layer 24 having the notch 21; the second opening 242 is disposed on an end of the insulating layer 24 opposite to the end having the notch 21.

The heat preservation layer 24 is internally provided with heat preservation materials, and a guide plate is arranged in a cavity formed by the heat preservation layer 24 and the outer wall of the culture device and used for guiding a heat transfer medium to transfer heat to the culture device. The guide plate plays roles of guiding the heat transfer medium, increasing the heat exchange area, improving the heat transfer efficiency and preventing dead angles and mixing in the culture process, can optimize the heat transfer performance of the heat transfer structure 2 and improves the efficiency and the stability of the culture device.

The insulating layer 24 may enhance the ability of the culture device to withstand external temperature changes. The thermal barrier 24 may slow the rate of change of temperature as the external temperature changes. And the heat transfer medium is introduced into the cavity formed by the heat preservation layer 24 and the culture device, so that the temperature in the culture device can be better controlled. The pipeline can rapidly introduce and discharge the heat transfer medium into the cavity, so that the heat transfer process is accelerated, and the heat transfer efficiency is improved.

In the present invention, a heat medium is introduced into the cavity through the first port 241 and is discharged through the second port 242. The temperature in the culture device can be adjusted by inputting heat transfer media of different temperatures through the first port 241. When the culture device needs the heat transfer structure 2 to transfer heat, the heat transfer medium enters the cavity through the first port 241, when the adventitious roots grow to the next stage and different environment temperatures are needed, the heat transfer medium is discharged through the second port 242, and when the heat transfer medium is completely discharged, a new heat transfer medium enters through the first port 241, so that the purpose of providing the adventitious roots with optimal temperature for the growth in different stages is achieved.

The water tank 5 is arranged outside the biological reaction device of plant tissues. The water tank 5 is connected to the water inlet pipe 52 and the water outlet pipe 51, and the heat transfer medium is supplied to the culture apparatus through the water inlet pipe 52 and is recovered to the water outlet pipe 51 through the second port 242.

The water has higher heat conduction performance and can transfer heat rapidly. When water flows in the cavity, heat can be quickly absorbed or released, an efficient heat transfer process is realized, and the water has good heat capacity and heat stability due to the characteristics of the water. By adjusting the temperature and the flow rate of water, the temperature in the culture device can be precisely controlled, and a stable growth environment is provided.

Because the first port 241 is located below the cavity, the water flow can impact the cavity from bottom to top, so that the surface of the cavity can be effectively cleaned, dirt on the cavity is flushed away, the maximization of the heat transfer effect is ensured, and the service life of the cavity is prolonged.

The notch 21 extends circumferentially, the detection device 11 comprises a temperature sensor, a PH detection device, a dissolved oxygen amount detection device and a sampling device, and the detection device 11 is circumferentially arranged on the tank body in the notch 21 at intervals.

The detection device 11 is directly arranged on the tank wall and comprises a temperature measuring device, an electrode PH measuring device, an electrode dissolved oxygen measuring device and a sampling device. The temperature change and the nutrition parameters in the culture device can be monitored in real time through the detection device 11, so that nutrition supply in the culture device can be adjusted in time, the normal growth of adventitious roots under the condition of obtaining sufficient nutrition support is ensured, the yield and the quality are improved, the stability of the temperature in a proper range can be ensured, and the negative influence of temperature fluctuation on the growth of the adventitious roots is avoided.

The detection device 11 comprises a plurality of detection units which are positioned in the notch 21 along the transverse interval, and the transverse extension length of the notch 21 on the culture tank 15 is basically consistent with that of the notch 21 on the seed tank 14.

The detection device 11 is located in the middle of the culture solution, extends inwards for ten centimeters from the tank wall, and the cutting structure 4 and the air inlet structure 3 can enable the culture solution to form an eddy current which rolls up and down, so that part of the culture solution detected by the detection device 11 is the most uniformly mixed part in the culture solution, the accuracy of a detection result is ensured, and the culture efficiency is improved.

The foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited to the above-mentioned embodiment, but is not limited to the above-mentioned embodiment, and any simple modification, equivalent change and modification made by the technical matter of the present invention can be further combined or replaced by the equivalent embodiment without departing from the scope of the technical solution of the present invention.

Claims

1. A plant tissue biological reaction device, characterized in that: comprising the steps of (a) a step of,

2. A plant tissue bioreactor apparatus according to claim 1, wherein: the culture device is provided with a first tank body and a second tank body which are communicated, and the second tank body is in an inverted cone shape;

3. A plant tissue bioreactor apparatus according to claim 2, wherein:

The culture device comprises a seed tank for cultivating plant tissues, wherein a first annular sleeve of the seed tank extends from the upper part of a first tank body of the seed tank to the edge of the first tank body connected with a second tank body; the second annular sleeve of the seed tank extends downwards from the lower end of the first annular sleeve to slightly exceed the connecting edge of the second tank body and the first tank body, and the thickness of the second annular sleeve of the seed tank gradually increases from top to bottom;

4. A plant tissue bioreactor apparatus according to any one of claims 2-3, wherein: the culture apparatus may further comprise a plate member,

the culture tank is circularly communicated with the seed tank through a seed shifting pipe and a seed returning pipe, the heat transfer structure is sleeved on the culture tank, a first annular sleeve and a second annular sleeve of the heat transfer structure are correspondingly sleeved on the periphery of the connecting end of a first tank body and a second tank body of the culture tank, the maximum inner diameter of the first tank body of the culture tank is far greater than the maximum inner diameter of the first tank body of the seed tank, and a notch of the heat transfer structure is formed by upwards extending the edge of the lower end of the second annular sleeve on the second annular sleeve.

5. A plant tissue bioreactor apparatus according to claim 4, wherein:

the first annular sleeve of the culture tank extends from the upper part of the first tank body of the culture tank to the edge of the connection between the first tank body and the second tank body;

6. A plant tissue bioreactor apparatus according to any one of claims 4-5, wherein:

the second tank body at the bottom of the seed tank is provided with at least two groups of air inlet structures for supplying air into the tank, and the air inlet structures are arranged below the heat transfer structure of the seed tank; the air inlet structure of the seed tank is oppositely arranged on the peripheral wall of the second tank body of the seed tank;

the second tank body at the bottom of the culture tank is provided with at least four groups of air inlet structures for supplying air into the tank, and the air inlet structures are arranged below the heat transfer structure of the culture tank; the inlet structure of the culture tank is circumferentially and uniformly distributed on the outer peripheral wall of the second tank body of the culture tank.

7. A plant tissue bioreactor apparatus according to claim 6, wherein:

two groups of air inlet structures of the seed tank extend into the second tank body, and the parts of the two groups of air inlet structures extending into the tank are provided with air inlet rods which are kept horizontal and have a certain length, so that sterile air is conveyed into the second tank body in a micro-bubble mode; the seed tank is characterized in that one group of air inlet structures are arranged right below the notch, and the other group of air inlet structures are arranged opposite to the air inlet structures below the notch.

8. A plant tissue bioreactor apparatus according to claim 6, wherein:

the four groups of air inlet structures of the culture tank extend into the second tank body obliquely upwards; the part of the air inlet structure extending into the tank is provided with an air inlet rod which is kept horizontal and has a certain length, and sterile air is conveyed into the second tank body of the culture tank in a bubble mode; the projections of any two opposite groups of air inlet rods in the tank body of the culture tank are approximately parallel, and the projections of two adjacent groups of air inlet rods in the tank body are approximately vertical;

9. A plant tissue bioreactor apparatus according to claim 8, wherein: at least two groups of cutting structures are arranged on the seed tank, and an air inlet structure on the seed tank and the cutting structures are arranged at intervals;

10. A plant tissue bioreactor apparatus according to any one of claims 4-9, wherein:

the air intake structure may include a plurality of air intake structures,

the fixed disc is rotatably arranged at the bottoms of the tank bodies of the culture tank and the seed tank;

the air guide bracket is arranged on the fixed disc;

the air inlet rod is sleeved at the other end of the air inlet pipe and is used for conveying air into the tank;

the air guide support comprises a first straight section, a second straight section and a third straight section, wherein the first straight section is perpendicular to the second straight section, an obtuse angle is formed between the second straight section and the third straight section, a horn mouth is formed in the first straight section, the periphery of the third straight section is sleeved with the air inlet rod, and the first straight section, the second straight section and the second straight section are in smooth transition.