CN117588575A

CN117588575A - Discharging assembly of biological culture device

Info

Publication number: CN117588575A
Application number: CN202311564511.1A
Authority: CN
Inventors: 张洪喜; 张明臣; 刘金坤; 高秀君; 刘冰
Original assignee: Shandong Anran Nanometre Ind Development Co ltd
Current assignee: Shandong Anran Nanometre Ind Development Co ltd
Priority date: 2023-11-22
Filing date: 2023-11-22
Publication date: 2024-02-23

Abstract

The invention provides a discharging component of a biological culture device, which is arranged at a material passing port at the bottom of a tank body and comprises: the valve core is matched with the shape of the material passing opening, is embedded in the material passing opening from the inner side of the bottom of the tank body, and is in smooth transition between the top surface of the valve core and the inner wall of the bottom of the tank body at the periphery of the material passing opening; and the valve rod is connected with the valve core and is used for driving the valve core to move along the axial direction of the material passing opening. According to the invention, the valve core is arranged to be matched with the shape of the material passing opening, and is embedded in the material passing opening, so that the top surface of the valve core is in smooth transition with the inner wall of the bottom of the tank body at the periphery of the material passing opening; the valve rod controls the valve core to reciprocate between the tank body and the material passing opening, so that the material passing opening is opened or closed, the tightness is ensured, and meanwhile, the damage of the valve core to an adventitious root is reduced.

Description

Discharging assembly of biological culture device

Technical Field

The invention belongs to the technical field of plant tissue culture devices, and particularly relates to a discharging component of a biological culture device.

Background

Along with the continuous improvement of living conditions, people generally pay more attention to strengthening physique through medicinal materials with nourishing effect by diet, and perform health care. Due to the huge population, the demand for medicinal plants has risen dramatically. In addition, the cultivation of medicinal plants by the traditional method not only requires a large amount of land and a long growth period, but also requires a proper climate. Any inappropriateness of conditions will limit and decrease the scale of cultivation of the medicinal plants and the yield of the medicinal plants.

Therefore, scientific workers develop a method and a culture device for large-scale cultivation by using isolated tissues or cells of plants. By combining the organs of plants: peeling roots, stems, leaves and the like, then placing the plant into a culture medium containing nutrient components for cultivation, simultaneously providing other environmental conditions such as temperature, illumination and the like suitable for growth, inducing organs of the plant into callus, adventitious buds and adventitious roots, finally, using the callus, the adventitious buds and the adventitious roots as seeds for cultivating the plant, and placing the plant into a cultivation device for cultivation. Furthermore, the defect that the cultivation of the medicinal plants depends on the conditions of land, climate and the like is overcome by culturing the adventitious roots through the culture device, and the yield of the medicinal plants can be improved by large-scale cultivation through a large number of culture devices.

At present, adventitious root culture generally controls the opening or closing of a feed port through arranging a diaphragm valve at the bottom of a tank, and the diaphragm valve can damage seeds cultivated in the tank in the use process, so that the yield is affected, and the working efficiency is reduced.

Therefore, designing a biological culture device discharging component to realize that the damage of the tank bottom valve to the adventitious root can be avoided when the adventitious root and the culture solution thereof are transferred becomes a problem to be solved urgently by the person skilled in the art.

In view of this, the present invention has been made.

Disclosure of Invention

The invention aims to solve the technical problems of overcoming the defects in the prior art and providing a discharging component of a biological culture device, which is arranged at a material passing hole at the bottom of a tank body and comprises a valve core and a valve rod, wherein the valve core is arranged to be matched with the shape of the material passing hole and is embedded in the material passing hole, so that the top surface of the valve core is in smooth transition with the inner wall of the bottom of the tank body at the periphery of the material passing hole; the valve rod controls the valve core to reciprocate between the tank body and the material passing opening, so that the material passing opening is opened or closed, and the problem that an indefinite root is damaged when the tank bottom valve is opened or closed in the prior art is solved.

In order to solve the technical problems, the invention adopts the basic conception of the technical scheme that:

a discharge assembly of a biological culture device, disposed at a feed port of a tank bottom, comprising:

the valve core is matched with the shape of the material passing opening, is embedded in the material passing opening from the inner side of the bottom of the tank body, and is in smooth transition between the top surface of the valve core and the inner wall of the bottom of the tank body at the periphery of the material passing opening;

and the valve rod is connected with the valve core and is used for driving the valve core to move along the axial direction of the material passing opening.

Further, the feed gap includes:

the opening is arranged at the bottom of the tank body and is matched with the shape of the valve core;

And the connecting wall extends downwards from the bottom of the tank body, is coaxial with the opening and is used for connecting the feeding pipe.

Further, the inside diameter of connecting wall is greater than the diameter of trompil, and the tip of inlet pipe is provided with:

the supporting part is spliced in the connecting wall and is abutted with the bottom of the tank body;

and the connecting part is arranged at one end of the supporting part far away from the tank bottom, extends outwards along the radial direction of the supporting part and is abutted with the lower end of the connecting wall.

Further, a connecting sealing ring is arranged between the connecting part and the connecting wall;

preferably, at least one side of the connecting sealing ring is provided with a sealing rib extending along the circumferential direction of the connecting sealing ring, and the connecting sealing ring is elastically abutted with the connecting wall and/or the connecting part through the sealing rib.

Further, a groove matched with the sealing rib is formed in the surface, contacted with the connecting sealing ring, of the connecting wall and/or the connecting part, and the groove is used for being clamped with the sealing rib;

preferably, the sealing rib is provided with at least one turn.

Further, the inner diameter of the feeding pipe is smaller than the diameter of the opening, one end of the feeding pipe, which is close to the tank body, is provided with a supporting wall with gradually increased diameter along the direction of being close to the tank body, and the inner wall of the opening is in transitional connection with the inner wall of the feeding pipe through the supporting wall;

the bottom of the valve core is attached to the supporting wall.

Further, the valve core includes:

the sealing gasket is arranged between the sealing part and the clamping part, at least part of the sealing gasket protrudes out of the peripheral wall of the valve core and is elastically abutted with the supporting wall;

preferably, the end of the valve rod is provided with a mounting part which extends along the axial direction of the valve rod and has a diameter smaller than the diameter of the valve rod;

the clamping part, the sealing gasket and the sealing part are sequentially sleeved on the mounting part along the direction away from the valve rod, and the sealing part is in threaded connection with the end part of the mounting part.

Further, the method comprises the steps of,

the feeding pipe below the tank body is provided with a corner,

the valve rod extends along the axial direction of the feed port, penetrates through the pipe wall of the feed pipe from the corner and extends to the outside of the feed pipe;

the lower part of the tank body is also provided with a driving component which is connected with one end of the valve rod far away from the valve core and is used for driving the valve rod to axially move along the material passing port.

Further, the automatic cutting device is further arranged, the adventitious roots are cut through the cutting device, after the cutting is completed, the valve core moves towards the inside of the tank body, the material passing opening is opened, and the adventitious roots are output from the material passing opening.

Further, the cutting device comprises a cutting part arranged in the cavity of the tank body and a power part capable of driving the cutting part;

the device also comprises a spacer bush arranged on the tank wall, wherein one side of the spacer bush is connected with the shearing part, and the other side of the spacer bush is connected with the power part and used for connecting the shearing part and the power part.

By adopting the technical scheme, compared with the prior art, the invention has the following beneficial effects.

1. According to the invention, the valve core is arranged to be matched with the shape of the material passing opening, and is embedded in the material passing opening, so that the top surface of the valve core is in smooth transition with the inner wall of the bottom of the tank body at the periphery of the material passing opening; the valve rod controls the valve core to reciprocate between the tank body and the material passing opening, so that the material passing opening is opened or closed.

2. According to the invention, the tank bottom valve is arranged outside the feeding pipe, so that damage of adventitious roots and culture solution to the tank bottom valve when the material passing opening is opened is avoided, and meanwhile, the flow rate inside the feeding pipe is increased.

3. According to the invention, the sealing gasket is arranged between the sealing part and the clamping part, and the valve rod passes through the clamping part and the sealing gasket and is rotationally fixed with the sealing part, so that the stability of the valve rod and the valve core is improved; the sealing gasket is in epitaxial butt joint on the supporting wall, so that the sealing performance of the valve core to the material passing opening is improved to a certain extent.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention. It is evident that the drawings in the following description are only examples, from which other drawings can be obtained by a person skilled in the art without the inventive effort. In the drawings:

FIG. 1 is a schematic diagram of the overall structure of the present invention;

FIG. 2 is a schematic diagram of a material passing hole structure in the invention;

FIG. 3 is a schematic diagram of the valve core and valve stem structure of the present invention;

FIG. 4 is a schematic view of a portion of a tube according to the present invention;

FIG. 5 is a schematic diagram of the overall structure of the embodiment;

FIG. 6 is a schematic view of a seed transfer box in an embodiment;

FIG. 7 is a schematic diagram of a bottom of a plant tissue culture tank according to an embodiment;

FIG. 8 is a schematic diagram of a cutting structure of a plant tissue culture tank according to an embodiment;

FIG. 9 is a schematic view of a cutting portion of a cutting structure in an embodiment;

FIG. 10 is a schematic view of an air intake structure of a plant tissue culture tank according to an embodiment;

FIG. 11 is a schematic cross-sectional view of an air intake structure in an embodiment;

fig. 12 is a schematic diagram of an air intake structure and a cutting structure at the bottom of a can body in an embodiment.

The main components in the figure are as follows:

1. a seed tank; 12. a culture tank; 2. a seed box is moved; 21. a feed inlet; 211. a feed pipe; 2111. a feed on-off valve; 2112. a support part; 2113. a support wall; 2114. a connection part; 2115. a first through pipe; 2116. a second through pipe; 2117. a spacer sleeve; 2118. a pipe clamp; a discharge port; 221. a discharge pipe; 2211. a discharging on-off valve; 2212. discharging plugs; 23. a seed moving port; 231. a seed transferring tube; 2311. a seed-moving on-off valve; 24. CIP port; 241. CIP tube; 2411. CIP on-off valve; 25. a sewage outlet; 251. a blow-down pipe; 2511. a sewage discharge on-off valve; 3. a recovery pipeline; 4. a high temperature steam line; 5. a material passing opening; 55. opening holes; 56. a connecting wall; 6. a valve core; 61. a sealing part; 62. a clamping part; 7. a valve stem; 71. a mounting part; 8. connecting a sealing ring; 81. sealing ribs; 82. a sealing gasket; 9. a drive assembly; 11. a tank wall; 20. a tank bottom; 90. a tank cavity; 31. a spacer bush; 311. a convex column; 312. a flange edge; 32. a shearing part; 321. a pin shaft; 322. a blade set; 323. a first blade; 324. a second blade; 325. a rotating end; 326. blade ends; 327. closing the side edges; 328. unfolding the side edges; 33. a transmission part; 331. a discharging section; 332. a drive section; 333. a discharge port; 34. a power section; 35. a guide cover; 351. an inlet; 352. an outlet; 353. a circulation port; 10. a plant tissue culture tank; 11. a tank wall; 40. an air intake structure; 41. an air inlet part; 42. an aeration section; 421. a cavity; 43. a conduit; 431. an air inlet section; 432. an air outlet section; 433. a transition section; 44. a seat plate; 45. quick release joint; 451. sealing grooves; 5. a material passing opening; 51. an interface; 52. stacking the pressing piece; 521. a through hole; 53. a hub kit; 531. a support ring; 532. a groove; 533. a connection hole; 54. a seal ring; 6. and (5) cutting the structure.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions in the embodiments will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present invention, and the following embodiments are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

In the description of the present invention, it should be noted that the azimuth or positional relationship indicated by the terms "inner", "outer", etc. are based on the azimuth or positional relationship shown in the drawings, and are merely for convenience of describing the present invention and simplifying the description, and are not indicative or implying that the apparatus or element in question must have a specific azimuth, be constructed and operated in a specific azimuth, and thus should not be construed as limiting the present invention.

In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "contacting," and "communicating" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.

As shown in fig. 1-4, in this embodiment, a discharging assembly of a biological culture device is described, wherein the discharging assembly of the biological culture device is disposed at a material passing port 5 at the bottom of a tank body, and includes: the valve core 6 is matched with the shape of the material passing opening 5, is embedded in the material passing opening 5 from the inner side of the bottom of the tank body, and smoothly transits between the top surface of the valve core and the inner wall of the bottom of the tank body at the periphery of the material passing opening 5; and the valve rod 7 is connected with the valve core 6 and is used for driving the valve core 6 to move along the axial direction of the feed port 5.

Specifically, the valve core 6 is used for controlling closing and opening of the material passing opening 5, the tank body is used for storing culture solution of adventitious roots and adventitious roots, the adventitious roots can flow to the next stage to continue culturing after being cultured in the pipe body for a period of time through the material passing opening 5, the tank bottom valve is arranged at the bottom of the tank body, the adventitious roots can flow to the next stage under artificial control, or a certain amount of adventitious roots can be cultivated by observing and recording how much time is needed for the adventitious roots, if the time for the adventitious roots to be cultivated is relatively stable, the opening or closing of the tank bottom valve can be controlled by setting fixed time through a program, and thus the labor cost can be greatly reduced.

Specifically, the material passing opening 5 includes: the opening 55 is arranged at the bottom of the tank body and is matched with the valve core 6 in shape; a connecting wall 52 extends downwardly from the bottom of the tank and is coaxial with the opening 55 for connecting to the feed tube 211.

Specifically, the inner diameter of the connecting wall 52 is larger than the diameter of the opening 55, and the end of the feed pipe 211 is provided with: a support portion 2112 inserted into the connecting wall 52 and abutting against the bottom of the can body; the connection portion 2114 is provided at an end of the support portion 2112 away from the tank bottom, and extends outward in the radial direction of the support portion 2112, and abuts against a lower end of the connection portion 2114.

Preferably, in this embodiment, the thickness of the end of the connecting wall 52 away from the can bottom is greater than the thickness of the end near the can bottom, and accordingly, the length of the connecting portion 2114 extending outwards is adapted to the thickness of the end of the connecting wall 52 away from the can bottom, and the greater the contact area between the connecting portion 2114 and the connecting wall 52 is, the more firmly the connecting portion 2114 and the connecting wall 52 are connected.

Specifically, a connecting sealing ring 8 is arranged between the connecting portion 2114 and the connecting wall 52, so as to strengthen the tightness between the connecting portion 2114 and the connecting wall 52 and make the connection between the connecting portion 2114 and the connecting wall more stable.

Preferably, at least one side of the connection sealing ring 8 is provided with a sealing rib 81 extending along the circumferential direction of the connection sealing ring 8, and the connection sealing ring 8 elastically abuts against the connection wall 52 and/or the connection portion 2114 through the sealing rib 81.

Specifically, the surface of the corresponding connecting wall 52 and/or the connecting portion 2114 contacting the connecting seal ring 8 is provided with a groove adapted to the sealing rib 81, and the groove is used for being clamped with the sealing rib 81.

Specifically, in this embodiment, the sealing ribs 81 are disposed on two sides of the connecting seal ring 8, and the arrangement of the sealing ribs 81 can effectively reduce the influence of the transverse force between the connecting wall 52 and the connecting portion 2114 on the fixing of the connecting wall 52 and the connecting portion 2114, and correspondingly, grooves corresponding to the sealing ribs 81 are disposed on the surface, contacting the connecting portion 2114 with the connecting wall 52, for clamping with the sealing ribs 81.

Specifically, in a possible embodiment, the sealing rib 81 may be provided in plurality, and the plurality of sealing ribs 81 are uniformly arranged from the inside to the outside along the circumferential direction of the connecting seal ring 8.

Specifically, the inner diameter of the feeding pipe 211 is smaller than the diameter of the opening 55, one end of the feeding pipe 211, which is close to the tank body, is provided with a supporting wall 2113 with gradually increased diameter along the direction of approaching the tank body, and the inner wall of the opening 55 is in transitional connection with the inner wall of the feeding pipe 211 through the supporting wall 2113; the bottom of the valve element 6 is attached to the support wall 2113.

Specifically, the valve element 6 includes: the sealing part 61 and the clamping part 62 arranged at the lower end of the sealing part 61 are provided with a sealing gasket 82 between the sealing part 61 and the clamping part 62, and the sealing gasket 82 at least partially protrudes from the peripheral wall of the valve core 6 and elastically abuts against the supporting wall 2113.

Preferably, the end of the valve rod 7 is provided with a mounting portion 71 extending in the axial direction of the valve rod 7 and having a diameter smaller than the diameter of the valve rod 7, and the engaging portion 62, the gasket 82 and the sealing portion 61 are sequentially sleeved on the mounting portion 71 in a direction away from the valve rod 7, and the sealing portion 61 is screwed with the end of the mounting portion 71.

Specifically, the valve core 6 is integrally matched with the opening 55 of the material passing hole 5, excessive transition between the top of the valve core 6 and the inner wall of the tank bottom is smooth, so that damage to an indefinite root in the tank when the material passing hole 5 is opened is reduced to the minimum, meanwhile, a sealing gasket 82 is additionally arranged between the sealing part 61 and the clamping part 62, on one hand, connection among the sealing part 61, the clamping part 62 and the sealing gasket 82 is more stable and firm, and on the other hand, the sealing gasket 82 is partially abutted against the supporting wall 2113, so that the sealing performance of the valve core 6 to the material passing hole 5 is improved.

Specifically, the feeding pipe 211 below the tank body is provided with a corner, the valve rod 7 extends along the axial direction of the feed port 5, penetrates through the pipe wall of the feeding pipe 211 from the corner and extends to the outside of the feeding pipe 211; the lower part of the tank body is also provided with a driving component 9 which is connected with one end of the valve rod 7 far away from the valve core 6 and is used for driving the valve rod 7 to move along the axial direction of the material passing port 5.

Specifically, the feeding pipe 211 is L-shaped, one end of the feeding pipe is connected to the bottom of the tank, and the other end of the feeding pipe extends to the tank used in the next stage, so that the cultured adventitious roots and adventitious root culture solution flowing out from the feed port 5 can be transferred into the culture tank for continuous culture.

Specifically, a first through pipe 2115 extends from the edge of the notch at the corner penetrating position to a direction away from the material passing hole 5, and the inner diameter of the first through pipe 2115 is larger than that of the valve rod 7; the valve rod 7 is disposed inside the first tube 2115 and includes a spacer 2117 disposed between the first tube 2115 and the valve rod 7, the spacer 2117 being in contact with the first tube 2115 and the valve rod 7.

Specifically, one end of the driving component 9, which is close to the material passing opening 5, is provided with a second through pipe 2116 extending from the middle part of the driving component 9 to the direction close to the material passing opening 5, and the inner diameter of the second through pipe 2116 is matched with the diameter of the valve rod 7; one end of the first through pipe 2115 far away from the material passing hole 5 is abutted and fixed with one end of the second through pipe 2116 near the material passing hole 5.

Specifically, one end of the first through pipe 2115 far away from the material passing opening 5 is provided with a flanging protruding outwards from the outer wall of the first through pipe 2115; the second through pipe 2116 is provided with a flange protruding outward from the outer wall of the second through pipe 2116 at one end near the material passing opening 5.

Specifically, the first through pipe 2115 and the second through pipe 2116 are fixedly connected through the pipe clamp 22, the pipe clamp 22 is formed by two semicircular castings, one ends of the two semicircular castings are connected together through a hinge structure, the other ends of the two semicircular castings extend outwards to form a fixing portion, through holes are formed in positions corresponding to the two fixing portions, screws can penetrate through the two through holes, nuts are screwed on the other sides of the screws, and the pipe clamp 22 is driven to shrink through locking of the screws and the nuts.

Specifically, the pipe clamp 22 is provided with a ring of grooves along the inner wall, the grooves are used for accommodating and fixing the flanges of the first through pipe 2115 and the second through pipe 2116, and the thickness of the grooves is matched with the sum of the flange thicknesses of the first through pipe 2115 and the second through pipe 2116.

Preferably, one surface of the flanging of the first through pipe 2115 is a straight surface extending from the end surface of the first through pipe 2115 away from the material passing hole 5 in a direction perpendicular to the axis of the first through pipe 2115, and the other surface is a cambered surface extending obliquely from the end point of the straight surface to the direction of approaching the material passing hole 5 to the outer wall of the first through pipe 2115; the flange surface of the second through pipe 2116 is a straight surface extending from the end surface of the second through pipe 2116 near the material passing opening 5 in a direction perpendicular to the axis of the second through pipe 2116, and the other surface is an arc surface extending obliquely from the end point of the straight surface to the outer wall of the second through pipe 2116 in a direction away from the material passing opening 5.

Specifically, the requirement of the flange on the thickness of the groove of the pipe clamp 22 is reduced, and the two through pipes can be fixedly locked as long as the thickness of the groove of the pipe clamp 22 is within a certain range.

Specifically, the first through pipe 2115 and the second through pipe 2116 are detachably connected through the pipe clamp, so that on one hand, the accessory is convenient to replace, on the other hand, the situation that the isolating sleeve 2117 is wrinkled when the isolating sleeve 2117 is installed under the condition that the through pipe is too long is avoided, meanwhile, the pipe clamp 22 selects a metal casting with higher strength, and the stability and strength of the first through pipe 2115 and the second through pipe 2116 when being fixed can be greatly improved.

Specifically, the driving component 9 needs to be disposed outside the feeding pipe 211, the whole feeding pipe 211 needs to be airtight, when the driving component is disposed in the feeding pipe 211, the wiring of the driving component 9 needs to be connected to the outside through the tank body, meanwhile, a large amount of adventitious roots and adventitious root culture solution needs to flow through the feeding pipe 211, the driving component 9 also fails under the condition that the liquid is soaked for a long time, the efficiency of the system is affected, further, the driving component 9 belongs to foreign matters relative to the feeding pipe 211, the existence of the driving component 9 can cause the adventitious roots and the adventitious root solution in the feeding pipe 211 to be insufficiently smooth during transferring, the adventitious roots can also hang on the driving component 9 to further reduce the feasible running of the feeding pipe 211, and finally, the feeding pipe 211 can also be blocked.

Specifically, in this embodiment, the driving assembly 9 is disposed outside the feeding pipe 211, and the driving assembly 9 is integrally connected to the feeding pipe 211 by disposing a through pipe, so that the opening or closing state of the feed gap 5 can be controlled while the driving assembly 9 is not disposed in the feeding pipe 211.

Specifically, the spacer 2117 is provided to ensure tightness between the valve rod 7 and the feeding pipe 211, when the feed port 5 is opened, the adventitious root in the tank body and the culture solution flow to the feeding pipe 211, the first through pipe 2115 is communicated with the feeding pipe 211, and when the liquid flows, the liquid easily flows into the driving assembly 9 along the gap to damage the device, on the other hand, the gap at the junction of the first through pipe 2115 and the feeding pipe 211 can cause the adventitious root to be hung at the gap, so that the adventitious root is damaged, and the adventitious root is accumulated at the junction of the first through pipe 2115 and the feeding pipe to affect the normal operation of the valve rod 7, so that the spacer 2117 is provided to avoid the occurrence of the above situation.

Specifically, in this embodiment, each connection position may be fixedly connected by using a connection sealing ring 8.

In particular, a diaphragm valve is generally used as a tank bottom valve, and the working principle of the diaphragm valve is to control medium circulation by utilizing a diaphragm, so that the medium is completely isolated from a valve body, and medium leakage and pollution are avoided. The diaphragm valve replaces the valve core 6 assembly with a valve body with a corrosion-resistant lining and a corrosion-resistant diaphragm, and the movement of the diaphragm is utilized to play a regulating role. The closing and opening of the pneumatic diaphragm valve is achieved by reinforcing the rubber diaphragm by pressure from the pipeline. When pressure medium enters the valve control cavity, the diaphragm sinks, and the valve channel is closed; when the control chamber pressure is vented to the atmosphere or downstream piping, the diaphragm floats upward and the valve passageway is opened. However, the diaphragm valve is not preferable in this embodiment because it may damage an adventitious root.

Specifically, in this embodiment, a lower expansion valve is selected, that is, the material passing opening 5 is opened when the valve core 6 moves toward the valve body; when the valve core 6 moves towards the direction far away from the valve body, the material passing opening 5 is closed, the situation that the valve core 6 is damaged is reduced by the cooperation of the valve core 6 and the material passing opening 5, but correspondingly, liquid in the tank body has a larger pressure to the tank bottom, the valve core 6 also needs to overcome the downward pressure of the liquid to the valve core 6 when the material passing opening 5 is closed, the tightness of the valve core 6 to the material passing opening 5 can not be ensured under the long-term use condition, and the risk of liquid seepage is caused.

Preferably, in this embodiment, the up-expanding valve is selected as the driving component 9, that is, the material passing opening 5 is closed when the valve core 6 moves towards the valve body; when the valve core 6 moves to the direction away from the valve body, the material passing opening 5 is opened, and through the cooperation of the valve core 6 and the material passing opening 5, on one hand, the valve core 6 is jacked up upwards, the condition that an indefinite root is damaged by the valve core 6 is avoided, and on the other hand, the pressure of liquid in the tank body can not cause gaps to appear between the valve core 6 and the material passing opening 5, so that the risk of liquid seepage is caused.

Specifically, after the adventitious roots in the tank body are cultivated, the adventitious roots need to flow out through the material port 5, the adventitious roots are discharged along the material inlet pipe 211, a high-temperature steam pipeline 4 is arranged on the material inlet pipe 211 and used for introducing high-temperature steam into the material inlet pipe 211 to disinfect the material inlet pipe 211 and the tank bottom valve, after the disinfection is finished, the driving assembly 9 is started to push the valve rod 7 upwards, the valve rod 7 moves away from the driving assembly 9 with the valve core 6, the material port 5 is opened, the adventitious roots flow to the material inlet pipe 211 from the material port 5, after a certain number of adventitious roots are discharged, the driving assembly 9 generates reverse acting force to pull the valve rod 7 downwards, the valve rod 7 moves towards the direction close to the driving assembly 9 with the valve core 6, and the material port 5 is closed.

Specifically, in the cultivation process, the problem that the adventitious roots are failed to be cultivated and rotten is caused by various reasons, the adventitious roots which are failed to be cultivated need to be finally discharged through the discharging component, and at the moment, a splitting device is needed to split the cultivated adventitious roots: the adventitious roots which are cultivated successfully flow to the other tank body through the shunt device; the adventitious roots which fail in cultivation are discharged through the flow dividing device, and the flow dividing device is arranged at the bottom of the other tank body, so that circulation is formed between the two tank bodies.

Specifically, as shown in fig. 5 and 6, in this embodiment, a biological culture apparatus is described, which is composed of two tanks, the bottom of the tank is provided with the discharging component, the two tanks are communicated by a feed pipe, and a seed moving box is arranged on the feed pipe, so as to realize the circulation of adventitious root cultivation.

Specifically, the biological culture device comprises a seed tank 1, a culture tank 12 and a seed transfer box 2 arranged between the seed tank 1 and the culture tank 12; the seed transfer box 2 includes: a feed port 21 communicating with the seed tank 1 through a feed pipe 211; a seed transfer port 23 which communicates with the culture tank 12 through a seed transfer pipe 231; the feeding pipe 211 and the seed shifter 231 are respectively provided with a feeding on-off valve 2111 and a seed shifter 2311, wherein the feeding on-off valve 2111 is a tank bottom valve in the discharging assembly.

Specifically, the seed moving box 2 is arranged at the lower part of the seed tank 1, a feed pipe 211 extending from the bottom of the seed tank 1 to the discharge port 22 is arranged at the feed port 21, and a feed on-off valve 2111 is arranged at the bottom of the seed tank 1 and outside the feed pipe 211 for opening or closing the feed pipe 211.

Specifically, the bottom of the seed tank 1 is configured to be conical, and is used for storing adventitious roots and adventitious root culture solution, the adventitious roots need to be cultured in the seed tank 1 for a period of time and then flow to the culture tank 12 for continuous culture, the bottom of the seed tank 1 is provided with the feeding on-off valve 2111, so that the feeding on-off valve 2111 can be manually controlled to flow to the culture tank 12 when the adventitious roots are cultured, or the time for recording a certain amount of adventitious roots can be observed and completed, if the time for completing the adventitious roots is relatively stable, the opening or closing of the feeding on-off valve 2111 can be controlled by setting fixed time through a program, and thus the labor cost can be greatly reduced.

Preferably, a circle of connecting sealing ring is arranged at the contact part of the valve core of the feeding on-off valve 2111 and the bottom of the seed tank 1, the volume of the tank body is larger, the pressure intensity of the tank body to the bottom of the tank body is larger, and if the valve core of the pneumatic valve is not tightly sealed with the seed tank 1, the situation of liquid seepage can occur.

Specifically, two thick pipes are arranged in the horizontal direction of the seed moving box 2 relative to the ground, and are respectively: a seed transfer pipe 231 having one end connected to the seed transfer port 23 and the other end extending to the culture tank 12 to be connected thereto; one end of the discharging pipe 221 is connected with the discharging hole 22, and the other end is communicated with the outside.

Specifically, after the preliminary cultivation of adventitious roots is completed, the adventitious roots need to enter the culture tank 12 to continue cultivation, flow into the seed transfer box 2 from the feed inlet 21, and then be transferred into the culture tank 12 from the seed transfer box 2 through the seed transfer pipe 231, and since the number of culture solution and adventitious roots in the seed tank 1 is large, a thicker pipeline is selected to reduce the time required for transferring from the seed tank 1 into the culture tank 12.

Specifically, the discharging pipe 221 is used for discharging the materials or the waste materials, the possibility of breeding failure can occur in the cultivation process of the seed tank 1, at this time, the culture solution and the adventitious roots in the culture solution can not be continuously transferred into the culture tank 12, the adventitious roots which are failed to cultivate are discharged through the discharging pipe 221, one end of the discharging pipe 221 is directly communicated with the outside, and the adventitious roots which are failed to cultivate can be directly discharged for treatment.

Specifically, one end that the discharging pipe 221 is far away from the seed box 2 is provided with a discharging plug 2212, the discharging plug 2212 can be set into a threaded casting, one end that the discharging pipe 221 is far away from the seed box 2 is provided with an internal thread matched with the thread of the discharging plug 2212, the discharging plug 2212 rotates to be sealed with the discharging pipe 221, a circle of connecting sealing ring is arranged on the contact surface of the discharging plug 2212 and the discharging pipe 221 to strengthen the sealing performance, and the discharging plug 2212 is arranged to ensure that the discharging pipe 221 is not polluted by the outside when not used, and the discharging pipe 221 is prevented from being polluted when discharging finished products.

Specifically, a discharge on-off valve 2211 is arranged at one end of the discharge pipe 221, which is close to the seed shifting box 2, and a valve core of the discharge on-off valve 2211 is matched with the inner diameter of the discharge pipe 221 and is clung to the inner wall, so that different liquids can flow in the seed shifting box 2, if the discharge on-off valve 2211 is far away from the seed shifting box 2, other liquids can enter corresponding pipelines, and various liquids can be mixed together when the pipelines are started, so that the stable operation of the biological culture device is not facilitated.

Specifically, a seed-shifting on-off valve 2311 is disposed at one end of the seed-shifting pipe 231 near the seed-shifting box 2, a valve core of the seed-shifting on-off valve 2311 is radially adapted to and tightly attached to the inner wall of the seed-shifting pipe 231, and a connecting sealing ring is disposed at a place where the valve core contacts the inner wall, so as to improve the tightness of the seed-shifting on-off valve 2311 to the seed-shifting pipe 231.

Specifically, the biological culture device further comprises a high-temperature steam pipeline 4 for conveying high-temperature steam, one or more high-temperature steam branch pipes are arranged at the output end of the high-temperature steam pipeline 4, and the high-temperature steam branch pipes are correspondingly communicated with the seed shifting pipe 231 and/or the feeding pipe 211 and/or the discharging pipe 221 and are used for disinfecting the seed shifting pipe 231 and/or the feeding pipe 211 and/or the discharging pipe 221;

Preferably, the end of the high temperature steam line 4 includes a first high temperature steam branch pipe communicating with an end of the transplanting pipe 231 remote from the transplanting box 2, a second high temperature steam branch pipe communicating with the discharge on-off valve 2211, the feed on-off valve 2111, and the transplanting on-off valve 2311, a third high temperature steam branch pipe, and a fourth high temperature steam branch pipe.

Specifically, the interior of the seed box 2 needs to be sterilized at high temperature before the adventitious roots are transferred from the seed tank 1 to the culture tank 12, so that the situation that the culture fails due to pollution of the adventitious root nutrient solution when the adventitious root nutrient solution flows through the seed box 2 is avoided.

Specifically, the seed transfer box 2 further comprises a drain outlet 25, and a drain pipe 251 is communicated with the drain outlet 25 and used for discharging the sterilized high-temperature steam;

preferably, the drain outlet 25 is arranged at the bottom of the seed moving box 2; a drain on-off valve 2511 is provided in the drain pipe 251.

Specifically, the seed transfer box 2 further includes a CIP port 24, and a CIP pipe 241 is connected to the CIP port 24 for discharging CIP cleaning liquid; the CIP pipe 241 is provided with a CIP on-off valve 2411.

Preferably, the CIP pipe 241 and the drain pipe 251 are provided as thin pipes, and further comprise a recovery pipe 3 for recovering high-temperature steam and/or CIP cleaning liquid, and the drain pipe 251 and/or the end of the CIP on-off valve 2411 away from the seed box 2 is communicated with the drain pipe 251.

Specifically, a CIP on-off valve 2411 is disposed at one end of the CIP pipe 241 near the CIP port 24, a valve core of the CIP dynamic valve is radially adapted to an inner diameter of the drain pipe 251 and is tightly attached to an inner wall, and a connection sealing ring is disposed at a place where the valve core contacts the inner wall, so as to improve tightness of the CIP on-off valve 2411 to the CIP pipe 241.

Specifically, a drain on-off valve 2511 is disposed on the drain 251, a valve core of the drain on-off valve 2511 is radially adapted to an inner diameter of the drain 251 and is tightly attached to an inner wall, and a connection sealing ring is disposed at a place where the valve core contacts the inner wall, so as to improve tightness of the drain on-off valve 2511 to the drain 251.

Specifically, before the culture solution and adventitious roots are added into the seed tank 1 in the first stage, the interior of the seed tank 1 is required to be sterilized, and in the invention, the CIP cleaning system is adopted to sterilize the seed tank 1, compared with the traditional cleaning mode, the CIP cleaning can save operation time and manpower, improve efficiency, rationalize production plan and improve productivity, save cleaning agent, steam, water and production cost, and increase the service life of machine parts.

Specifically, the waste liquid generated after the CIP cleaning of the seed tank 1 is discharged from the feed port 21 into the seed transfer tank 2, and is discharged from the seed transfer tank 2 into the recovery line 3 through the CIP pipe 241.

Specifically, in this embodiment, the five channels connected to the seed transfer box 2 are all provided with valves for controlling the opening or closing of the corresponding pipelines, so that on one hand, the trend of the substances in the seed transfer box 2 can be controlled by opening or closing the valves, and the risk that the adventitious root nutrient solution flows to other pipelines or waste liquid flows into the culture tank 12 is avoided.

Specifically, in the adventitious root cultivation process, along with the increase of cultivation time, the adventitious root can become longer along with the increase of time, and the adventitious root after becoming longer can cause the jam more easily when opening the feed gap 5 or transplanting, is unfavorable for the continuous cultivation of adventitious root, therefore after the adventitious root is cultivated for a period of time, need cut the adventitious root, cut after accomplishing, feed gap 5 opens, and the adventitious root flows to the next cultivation stage through transplanting box 2 to make the adventitious root can not jam each pipeline in the transplanting process.

In the present invention, as shown in fig. 7, a cutting structure 6 for a plant tissue culture pot is described. The plant tissue culture tank is provided in a shape of a revolution body with its central axis in a vertical direction. The tank wall 11 of the plant tissue culture tank comprises at least a portion extending from the top down and gradually sloping towards the central axis of the plant tissue culture tank. The portion forms an inverted cone.

Preferably, the tank wall 11 also comprises a portion which is cylindrical parallel to the central axis. The cylindrical portion is connected with the inverted cone portion to form the whole plant tissue culture tank, and the inverted cone portion is arranged below to form the tank bottom 20 of the plant tissue culture tank. The cutting structure 6 is arranged in the inverted cone, i.e. the spacer 31 is arranged at the can bottom 20,

in this embodiment, the cutting structure 6 is arranged at the bottom 20 of the pot to cut the adventitious roots rapidly, so that the adventitious roots in the plant tissue culture pot are smooth in cut, have the same growth state, facilitate the periodic pruning of the adventitious roots in the plant tissue culture pot, promote the circulation of nutrient solution in the plant tissue culture pot, maintain good growth conditions of the adventitious roots, and improve the cultivation efficiency and quality of the adventitious roots in the plant tissue culture pot.

As shown in fig. 8, the present embodiment is directed to a cutting structure 6 of a plant tissue culture tank. The cutting structure 6 is detachably connected to the tank wall 11 of the plant tissue culture tank.

Specifically, the cutting structure 6 includes a cutting portion 32 and a power portion 34, and the power portion 34 can drive the cutting portion 32 to cut adventitious roots. The power portion 34 is not directly connected to the shear portion 32, the shear portion 32 is disposed within the tank cavity 90 of the plant tissue culture tank, and the power portion 34 is disposed outside the plant tissue culture tank. The power section 34 and the shear section 32 are spaced apart from the tank wall 11 of the plant tissue culture tank.

In particular, the plant tissue culture tank is also provided with a spacer 31. The wall 11 of the plant tissue culture tank is provided with a circular mounting hole, and the outer peripheral surface of the spacer bush 31 is cylindrical and has the same diameter as the mounting hole. The spacer 31 is removably inserted into a mounting hole in the tank wall 11.

One side of the spacer 31 is connected with the shearing part 32, and the other side is connected with the power part 34, so that the shearing part 32 and the power part 34 are separated inside and outside the plant tissue culture tank. The shear part 32 is mounted on the side of the spacer 31 facing the tank cavity 90, and the power part 34 is mounted on the side of the spacer 31 facing the outside of the plant tissue culture tank. The cutting structure 6 can be simply and quickly arranged on the plant tissue culture tank by directly inserting the spacer bush 31 into the cutting part 32 from the mounting hole when the cross section of the spacer bush 31 is larger than that of the cutting part 32.

Spacer 31 has a variety of patterns and in some embodiments of the invention spacer 31 is annular and formed with an annular groove. When the spacer bush 31 is annular, the middle part of the spacer bush 31 is a thin-wall plate, and an annular groove formed by sinking towards one side of the plate surface is arranged on the outer circumference of the thin-wall plate.

In other embodiments, spacer 31 is cylindrical and formed with a cylindrical recess.

The cavity opening of the spacer 31 is always located at the end face of the spacer 31. In some embodiments, the spacer 31 is integral with the tank wall 11 and is formed by recessing the tank wall 11 into the cavity of the tank. At this time, the groove opening of the spacer 31 is formed on the surface of the can.

In other embodiments, the spacer 31 is a separate piece that is separable from the tank wall 11. The spacer 31 may be removably attached to the tank wall 11 by fasteners or may be secured to the tank wall 11 by adhesive or welding.

In this embodiment, be provided with in plant tissue culture jar and cut out structure 6 that can tailor the adventitious root, still set up spacer 31 between shearing portion 32 and power portion 34 simultaneously for cut out structure 6 dispersedly installs in plant tissue culture jar's jar wall 11, the outside, thereby both can maintain and promote the cultivation efficiency of adventitious root through cutting structure 6 control adventitious root's length, still can avoid microorganism or impurity to get into in the plant tissue culture jar through cutting structure 6, improved plant tissue culture jar's leakproofness.

In another embodiment of the invention, shown in fig. 8, a spacer 31 of a cutting structure 6 is described. The spacer 31 can make the power part 34 better attract from one side of the spacer 31 and drive the shearing part 32 to perform cutting action, thereby simplifying the structure of mutual attraction between the shearing part 32 and the power part 34 and increasing the attraction of the power part 34 to the shearing part 32.

Specifically, the spacer 31 protrudes from the surface of the tank wall 11 toward the tank cavity 90 along the vertical direction of the plant tissue culture tank wall 11. The spacer 31 forms a groove on the other side, corresponding to the position where the spacer 31 protrudes toward the tank cavity 90, as seen from the outside of the plant tissue culture tank wall 11.

In particular, the shearing part 32 is connected with the outer convex surface of the spacer 31, and the power part 34 is installed at the groove. Preferably, the shearing part 32 is provided with a member which is sleeved on the periphery of the spacer 31 and can rotate relative to the spacer 31, and the power part 34 attracts and drives the member from the other side of the spacer 31 through the spacer 31, so as to drive the shearing part 32 to cut the adventitious roots.

In this embodiment, by optimizing the shape of the spacer 31, the spacer 31 protrudes into the cavity 90 of the can body, so as to enhance the attraction and driving effect of the power portion 34 on the shearing portion 32, ensure that the cutting structure 6 shears the adventitious roots better, avoid the adventitious roots from winding and agglomerating, and promote the faster growth of the adventitious roots.

In another embodiment of the invention, shown in fig. 8, a spacer 31 of another cutting structure 6 is described. Unlike the spacer 31 in the previous embodiment, the outer circumference of the spacer 31 is provided with an annular groove recessed in the direction of the central axis thereof, and the middle portion of the spacer 31 is located between the groove bottom and the groove top in the direction of the central axis of the annular groove.

Preferably, the middle of spacer 31 is flush with the top of the tank, i.e. the middle of spacer 31 is flush with the surface of tank wall 11 of the plant tissue culture tank.

Specifically, when the spacer 31 is disposed on the plant tissue culture tank, the annular groove of the spacer 31 extends from the surface of the tank wall 11 to the tank body cavity 90 along the vertical direction of the plant tissue culture tank wall 11, and the notch of the annular groove is located on the surface of the tank wall 11 of the plant tissue culture tank, so that the spacer 31 forms an annular structure with the annular groove.

Preferably, the middle part of the spacer 31 protrudes toward the tank cavity 90 to form a cylindrical structure with a cylindrical groove. In this embodiment, by optimizing the shape of the spacer 31, the spacer 31 protrudes into the cavity 90 of the can body, so as to enhance the attraction and driving effect of the power portion 34 on the shearing portion 32, ensure that the cutting structure 6 shears the adventitious roots better, avoid the adventitious roots from winding and agglomerating, and promote the faster growth of the adventitious roots.

In another embodiment of the present invention, as shown in FIG. 8, a spacer 31 is described that is removably attachable to a plant tissue culture tank. The tank wall 11 is provided with a mounting hole, and if the spacer bush 31 is annular, the annular groove of the spacer bush is embedded into the tank cavity 90 and is fixed on the mounting hole; if the spacer 31 is barrel-shaped, it is fixed to the mounting hole in such a manner that the barrel bottom is fitted into the cavity 90 of the can body.

Specifically, a mounting hole is formed in the wall 11 of the plant tissue culture tank, and the outer peripheral surface of the spacer 31 is formed in a cylindrical shape and has the same diameter as the mounting hole. The spacer 31 is removably inserted into a mounting hole in the tank wall 11.

In this embodiment, one side of the spacer 31 is connected to the shear part 32, and the other side is connected to the power part 34, so that the shear part 32 and the power part 34 are separated from each other inside and outside the plant tissue culture pot. The shear section 32 is mounted within the tank cavity 90 and the motive section 34 is mounted externally of the plant tissue culture tank. The cutting portion 32 can be placed directly into the tank cavity 90 of the plant tissue culture tank from the mounting hole, thereby enabling the cutting structure 6 to be simply and quickly disposed on the plant tissue culture tank.

In another embodiment of the present invention, as shown in FIG. 7, a plant tissue culture pot and spacer 31 mounting position is described.

The plant tissue culture tank is provided in a shape of a revolution body with its central axis in a vertical direction. The tank wall 11 of the plant tissue culture tank comprises at least a portion extending from the top down and gradually sloping towards the central axis of the plant tissue culture tank. The portion forms an inverted cone.

Preferably, the tank wall 11 also comprises a portion which is cylindrical parallel to the central axis. The cylindrical portion is connected with the inverted cone portion to form the whole plant tissue culture tank, and the inverted cone portion is arranged below to form the tank bottom 20 of the plant tissue culture tank. The spacer 31 is arranged in the inverted cone-shaped part, i.e. the spacer 31 is arranged at the tank bottom 20, so that the cutting structure 6 is distributed at the tank bottom 20 of the plant tissue culture tank.

In this embodiment, the plant tissue culture tank is provided with the tank bottom 20 in an inverted cone shape, so that the pressure of the nutrient solution on the adventitious roots can be reduced, and the adventitious roots can grow. And the bottom 20 of the reverse taper can play a role in converging adventitious roots suspended in nutrient solution, so that the spacer 31 and the cutting structure 6 are arranged on the bottom 20 of the reverse taper, and the cutting structure 6 can intensively and efficiently cut the adventitious roots.

In other embodiments of the present invention, as shown in fig. 9, a cutout 32 of a cutting structure 6 is described. The shear 32 includes a first blade 323 and a second blade 324. Wherein the first blade 323 and the second blade 324 are disposed adjacent to each other and are connected in series by a rotation axis perpendicular to the adjacent surfaces. The first blade 323 and the second blade 324 can rotate freely around the rotating shaft, and the opposite sides of the two blades form a continuously opened and closed notch which can be used for shearing adventitious roots.

Alternatively, the first blade 323 is fixedly connected, and the second blade 324 rotates about the rotation axis relative to the first blade 323.

More preferably, the first blade 323 is fixedly connected with the spacer 31, and the second blade 324 is sleeved with the spacer 31 and can rotate relative to the central axis of the spacer 31. Meanwhile, the power part 34 is provided with a rotor which moves circularly in the groove. A magnetic attraction member is mounted on the rotor to attract the second blade 324 through the spacer 31.

In this embodiment, the shearing portion 32 is formed by two blades that can rotate relatively, and forms a constantly open-shut incision by the two blades, and the adventitious root is sheared through the incision to make the adventitious root incision flush, so that the dragging and tearing of the adventitious root are avoided, the success rate of the adventitious root shearing is improved, necrosis after the adventitious root is cut is avoided, and the yield and quality of the adventitious root are improved.

In another embodiment of the present invention, as shown in fig. 8, a shear section 32 is described that is capable of cutting out fixed length adventitious roots.

The shear 32 includes at least two axially spaced blade sets 322. The blade set 322 includes the first blade 323 and the second blade 324 with their sides in contact. The spacing between adjacent blade sets 322 is between 10mm and 15 mm.

Specifically, all the first blades 323 in the blade set 322 are parallel to each other and are fixed with the rotating shaft, all the second blades 324 are parallel to each other and are connected at the ends of the second blades 324 in the length direction of the second blades to form a frame body coated on the outer side of the first blades 323, and the second blades 324 rotate relative to the first blades 323 to form a continuously opened and closed notch.

In this way, when the second blade 324 is driven to rotate relative to the first blade 323, the adjacent blade group 322 simultaneously shears the adventitious root entering the shearing portion 32 so that the length of the cut adventitious root coincides with the interval between the adjacent blade groups 322.

In this embodiment, the shearing part 32 is provided with multiple groups of blades, and the blade groups 322 are arranged according to fixed intervals, so that adventitious roots with consistent length can be obtained by cutting adventitious roots, the adventitious roots in the plant tissue culture tank have the same growth state, the nutrient content of nutrient solution can be conveniently and regularly adjusted, the circulation of the nutrient solution in the plant tissue culture tank is promoted, stable growth conditions are maintained, and the cultivation efficiency and the quality of the adventitious roots are improved.

In another embodiment of the present invention, as shown in fig. 8, a cutting structure 6 of a plant tissue culture tank is described. The cutting structure 6 is further provided with a transmission portion 33 between the cutting portion 32 and the power portion 34.

Specifically, the transmission part 33 is cylindrical, and is sleeved on the spacer 31. The inner cylinder surface of the transmission part 33 and the outer circumferential surface of the spacer 31 may be slidably connected, or may be rotatably connected and connected together by a bearing. In particular, the transmission portion 33 can rotate around the spacer 31. The transmission part 33 is connected with the second blade 324 and rotates synchronously, and the power part 34 drives the shearing part 32 to cut the adventitious roots through the transmission part 33.

In another embodiment of the present invention, shown in fig. 9, a pod 35 of a cutting structure 6 is described. The plant tissue culture tank is internally provided with a cutting structure 6, so as to better promote the circulation of adventitious roots in the tank cavity 90, avoid repeated cutting of a part of adventitious roots by the cutting structure 6, and further provide a guide cover 35 for guiding liquid flow on the cutting structure 6.

Specifically, the pod 35 is a cylindrical thin-walled tube and is provided with cavities penetrating both ends in the direction of the central axis thereof.

The pod 35 is fitted around the outer periphery of the cutout 32. One end of the pod 35 protrudes from the cutout 32 and has an inlet 351 at the end face. The inlet 351 is parallel to the circular face formed by the rotation of the second blade 324. The other end of the deflector 35 extends in the direction of the tank wall 11 and is provided with an outlet 352 at the end face. The outlet 352 is sleeved on the periphery of the transmission part 33, so that the air guide sleeve 35 at least partially covers the transmission part 33.

Specifically, the sidewall of the air guide sleeve 35 is further provided with a circulation port 353. When the air guide sleeve 35 is sleeved on the cutting structure 6, one end of the circulating opening 353 is aligned with the cutting portion 32 along the axial direction of the cutting structure 6, and the other end is close to the outlet 352.

Specifically, the adventitious roots enter the guide cover 35 from the inlet and are cut by the cutting part 32, the cut adventitious roots flow out through the circulation port 353 formed in the side wall of the guide cover 35, the feeding on-off valve 2111 is opened, and the cut adventitious roots flow into the seed transfer box 2 and finally flow to the culture tank 12.

In this embodiment, the dome 35 is disposed on the outer periphery of the cutting portion 32, so that the circulating flow area of the nutrient solution in the plant tissue culture tank is increased, the adventitious roots are promoted to circulate in the tank cavity 90 in a large range, the adventitious roots in the area near the cutting portion 32 are prevented from being influenced by the cutting portion 32 to circulate in a small range, and the plant tissue culture tank is ensured to be in a good circulating state, so that the adventitious roots can be trimmed.

Specifically, the density of adventitious roots is greater than the density of culture solution, if be in under the static state for a long time in the jar body, can lead to the adventitious roots to pile up in jar body bottom, on the one hand, pile up the contact of adventitious roots and culture solution in jar body bottom and diminish, be unfavorable for the growth and development of adventitious roots, on the other hand, the adventitious roots is piled up in jar bottom, when the feeding on-off valve is opened, cause the jam easily, on the other hand, the adventitious roots too gathers also be unfavorable for cutting device to cut it too, consequently, need increase air inlet structure in jar body, make the adventitious roots distribute more evenly, blow the adventitious roots to cutting assembly and cut in the air inlet process.

Specifically, as shown in FIG. 10, in the present invention, an air intake structure 40 provided on a plant tissue culture tank 10 is described. The air intake structure 40 includes an air intake portion 41, an aeration portion 42, and a conduit 43 connecting the air intake portion 41 and the aeration portion 42. Wherein the conduit 43 is formed in a straight bar shape, and the air inlet portion 41 and the aeration portion 42 are connected to both ends of the conduit 43, respectively.

In particular, the center of the aeration portion 42 is provided with a cavity 421. The cavity 421 communicates with the conduit 43 and the wall of the cavity 421 is provided with micropores communicating from the outside of the aeration portion 42 into the cavity 421. A large number of micropores are densely and uniformly distributed throughout the cavity wall.

The aeration portion 42 may be made of a ceramic material or a polymer material.

Preferably, the aeration portion 42 is made of a titanium alloy material such that adventitious roots cannot adhere to the outer surface of the aeration portion 42, thereby avoiding adventitious root accumulation spoilage.

In this example, a large number of micropores are densely formed on the side wall of the aeration portion 42, and all the micropores are communicated with the cavity 421 formed in the center of the aeration portion 42, so that gas entering the cavity 421 of the aeration portion 42 through the gas guide pipe can be sprayed into the solution from all the micropores at the same time, thereby forming a large number of small bubbles in the solution at the same time, the small bubbles move outwards from the outer surface of the aeration portion 42 and float upwards, so that the small bubbles are prevented from being polymerized into large bubbles, the contact and dissolution rate of the gas and the solution are improved, and the circulating flow of the solution is promoted by the floating movement of a large number of small bubbles in the solution in a dispersing manner, and the dissolved oxygen effect is further improved.

In another embodiment of the present invention, as shown in fig. 11, an aeration portion 42 of an air intake structure 40 is described. In order to reduce the time for opening the micropores in the aeration portion 42 and to ensure a more uniform distribution of micropores in the aeration portion 42, the air can be decomposed into small bubbles dissolved in the culture medium. The aeration section 42 is provided by stacking a large number of small particles smaller than 1mm in diameter and sintering them together to constitute a housing having a cavity 421 in the center.

The small particles may be ceramic materials, polymer materials, or metal alloy materials.

Specifically, the small particles are set to spherical shapes. A large number of small particles are closely adhered and there are gaps between adjacent small particles. The gaps are arranged in sequence and connected in the radial direction of the aeration portion 42 to form micropores extending from the inner surface of the chamber wall to the outer surface of the chamber wall. Thus, the outer surface of the aeration portion 42 is covered with micropores, similar to the meshes of a screen.

Preferably, the small particles are made of a metallic titanium alloy, so that the adhesion of adventitious roots to the outer surface of the aeration portion 42 can be avoided.

In this embodiment, the aeration portion 42 is provided by a method of sintering and adhering small particles, so that the distribution of micropores formed on the aeration portion 42 is more uniform, and therefore, air can be decomposed into small bubbles dissolved in the culture solution better, which is beneficial to increasing the dissolution rate of air in the culture solution.

In another embodiment of the present invention, as shown in FIG. 11, an air intake structure 40 is described that can be more conveniently mounted to a culture tank.

Specifically, the aeration portion 42 provided with the air intake mechanism has a long cylindrical shape, and one end of the aeration portion 42 is connected to the conduit 43. The central axis of the aeration portion 42 is arranged to coincide with the central axis of the tip of the conduit 43 and to extend in a straight line in a direction away from the conduit 43. Meanwhile, the conduit 43 is long and straight, so that the aeration portion 42 and the conduit 43 are arranged to coincide with the central axis.

In this embodiment, the aeration portion 42 is formed in a long cylindrical shape and extends outward from the end of the conduit 43, so that the diameter of the opening formed in the culture tank can be reduced, and the contact surface between the air inlet structure 40 and the culture medium can be made larger, which is advantageous in sufficiently dispersing and dissolving air in the culture medium.

In another embodiment of the present invention, as shown in fig. 11, an air intake structure 40 is described that can be more conveniently secured. A seat plate 44 provided on the air intake structure 40 is located in the middle of the air intake portion 41 and the aeration portion 42, separating the air intake portion 41 and the aeration portion 42.

Specifically, a seat plate 44 is attached to the duct 43 and extends radially outwardly of the duct 43, forming a shape in which the duct 43 passes vertically through the center of the seat plate 44. In particular, the seat plate 44 is located between both ends of the duct 43, the aeration portions 42 are distributed on one side of the seat plate 44, and the air intake portion 41 is disposed on the other side of the seat plate 44.

Preferably, the seat plate 44 is provided in a circular flat plate shape so that the air intake structure 40 can be rotated around the duct 43 when installed.

In this embodiment, the seat board 44 is disposed on the air inlet structure 40, so that the air inlet structure 40 and the culture tank are more convenient to install and connect, and the air inlet structure 40 can rotate around the guide pipe 43, so that the difficulty of assembly work is reduced.

In another embodiment of the present invention, as shown in fig. 11, an intake structure 40 with improved intake performance is described. The conduit 43 of the air intake structure 40 is optimally adjusted for better dissolution of the air into the culture medium.

Specifically, the setting conduit 43 includes an elongated straight intake section 431. The air inlet sections 431 are respectively suspended to at least one length toward both sides of the seat plate 44, thereby facilitating the better connection and fixation of the aeration section 42 and the air inlet section 41 to the guide duct 43.

The aeration portion 42 and the air inlet portion 41 are respectively connected with the ends of the air inlet section 431 which extend out of the side surface of the seat plate 44, so that the aeration portion 42 and the air inlet portion 41 are separated on two sides of the seat plate 44.

In another embodiment of the present invention, in order to increase the position of the aeration section 42 in the culture tank after being mounted to the culture tank, the guide pipe 43 is further provided with an air outlet section 432 disposed at an angle with respect to the air inlet section 431.

An air outlet section 432 has one end connected to the air inlet section 431 and the other end connected to the aeration section 42. In particular, the angle between the outlet section 432 and the inlet section 431 is set to be greater than 90 °.

In another embodiment, the conduit 43 is further provided with a transition 433 connected between the inlet section 431 and the outlet section 432 in order to further widen the position range of the aeration section 42 in the culture tank in cooperation with the seat plate 44.

In particular, the transition 433 is arranged parallel to the seat plate 44. One end of the transition section 433 is connected to the air inlet section 431, and the other end is connected to the air outlet section 432. The air outlet section 432 is disposed obliquely to the transition section 433 and extends from the transition section 433 in a direction away from the seat plate 44.

In this embodiment, the optimizing conduit 43 includes a plurality of straight sections and the adjacent straight sections are connected in a bending manner, so that when the air inlet structure 40 is installed on the culture tank, the aeration portion 42 is ensured to be located at more positions of the culture tank by rotating the air inlet structure 40, so that the hoverable position range of the aeration portion 42 in the culture tank is enlarged, and bubbles can be fully diffused into the culture solution from the periphery of the aeration portion 42.

In another embodiment of the present invention, as shown in fig. 10 and 11, an intake structure 40 capable of quick connection is introduced. The air intake portion 41 of the air intake structure 40 includes a quick disconnect 45.

Specifically, a medium speed disconnect 45 is provided at the end of the conduit 43 in the air intake portion 41. The quick release connector 45 is conical in shape. The conical bottom surface of the quick release connector 45 is superposed on the air inlet end surface of the duct 43. The inlet of the duct 43 is located in the center of the bottom surface. The tapered surface of the quick release connector 45 is directed toward the seat plate 44 and gradually contracted to the outer peripheral surface of the guide tube 43.

In another embodiment of the present invention, as shown in fig. 10, a quick disconnect 45 having a sealed air intake structure 40 is described.

The conical bottom surface of the quick disconnect 45 is provided with a seal groove 451. The seal groove 451 is recessed from the bottom surface toward the inside of the quick release connector 45 and forms a recess on the bottom surface. In particular, a seal groove 451 surrounds the outer periphery of the inlet of said duct 43.

In this embodiment, the plant tissue culture tank 10 is provided with the air inlet structure 40, so that a large number of small bubbles can be formed in the solution by air when adventitious roots are cultivated, the small bubbles move outwards and upwards from the aeration portion 42 to promote the circulation flow of the nutrient solution, and the large number of small bubbles are dispersed in the solution to further improve the contact and dissolution rate of the air and the nutrient solution, provide stable conditions for the proliferation of the adventitious roots, and facilitate the improvement of the cultivation efficiency and quality of the adventitious roots.

The height of the air intake structure 40 at the bottom of the culture tank 10 of the present embodiment is defined as a first height, and the height of the cutting structure 66 at the bottom of the culture tank 10 is defined as a second height.

As shown in fig. 12, the air intake structure 40 and the cutting structure 6 are located at the same height of the bottom of the culture tank 10, i.e., the first height is equal to the second height.

As shown in fig. 12, the air intake structure 40 and the cutting structure 6 are located at different heights from the bottom of the culture tank 10, and the cutting structure 6 is disposed at a position where the second height is greater than the first height.

As shown in fig. 12, the air intake structure 40 and the cutting structure 6 are located at different heights from the bottom of the culture tank 10, and the air intake structure 40 is disposed at a position where the first height is greater than the second height.

Specifically, the cutting structure 6 is arranged at a position with the second height being larger than the first height, and the air inlet structure 40 is arranged at a position with the first height being larger than the second height, so that a preset gap is reserved between the aeration part 42 and the cutting structure 6, the callus is ensured to be conveniently cut into small sections with a certain length by the cutting structure 6, the distance between the aeration part 42 and the cutting structure 6 is prevented from being too short, and sterile air entering the culture tank 10 from micropores on the surface of the aeration part 42 influences the cutting of the callus.

The foregoing description is only illustrative of the preferred embodiment of the present invention, and is not to be construed as limiting the invention, but is to be construed as limiting the invention to any simple modification, equivalent variation and variation of the above embodiments according to the technical matter of the present invention without departing from the scope of the invention.

Claims

1. The utility model provides a biological culture device's ejection of compact subassembly, sets up in the material mouth (5) department of crossing of jar body bottom, its characterized in that includes:

the valve core (6) is matched with the shape of the material passing opening (5), is embedded in the material passing opening (5) from the inner side of the bottom of the tank body, and smoothly transits between the top surface of the valve core and the inner wall of the bottom of the tank body at the periphery of the material passing opening (5);

the valve rod (7) is connected with the valve core (6) and is used for driving the valve core (6) to move along the axial direction of the feed port (5).

2. A discharge assembly of a biological culture device according to claim 1, characterized in that the feed gap (5) comprises:

the opening (55) is arranged at the bottom of the tank body and is matched with the valve core (6) in shape;

a connecting wall (52) extending downwards from the bottom of the tank body and coaxial with the opening (55) for connecting the feed pipe (211).

3. A discharge assembly of a biological culture device according to claim 2, characterized in that the inside diameter of the connecting wall (52) is larger than the diameter of the opening (55), the end of the feed pipe (211) being provided with:

a support part (2112) inserted in the connecting wall (52) and abutting against the bottom of the tank body;

and a connection portion (2114) provided at an end of the support portion (2112) remote from the tank bottom, extending outward in the radial direction of the support portion (2112), and abutting against the lower end of the connection wall (52).

4. A discharge assembly of a biological culture device according to claim 3, characterized in that a connection sealing ring (8) is arranged between the connection part (2114) and the connection wall (52);

at least one side of the connecting sealing ring (8) is provided with a sealing rib (81) extending along the circumferential direction of the connecting sealing ring (8), the sealing rib (81) is at least provided with one circle, and the connecting sealing ring (8) is elastically abutted with the connecting wall (52) and/or the connecting part (2114) through the sealing rib (81).

5. The discharge assembly of a biological culture device according to claim 4, wherein the surface of the connecting wall (52) and/or the connecting portion (2114) contacting the connecting sealing ring (8) is provided with a groove adapted to the sealing rib (81), and the groove is used for clamping with the sealing rib (81).

6. The discharging assembly of a biological culture device according to any one of claims 1 to 5, wherein an inner diameter of the feeding pipe (211) is smaller than a diameter of the opening (55), a supporting wall (2113) with gradually increased diameter along a direction approaching the tank body is arranged at one end of the feeding pipe (211) approaching the tank body, and the inner wall of the opening (55) is in transitional connection with the inner wall of the feeding pipe (211) through the supporting wall (2113);

the bottom of the valve core (6) is attached to the supporting wall (2113).

7. The discharge assembly of a biological culture device according to claim 6, wherein the valve cartridge (6) comprises:

A sealing part (61) and a clamping part (62), a sealing gasket (82) is arranged between the sealing part (61) and the clamping part (62), and the sealing gasket (82) at least partially protrudes out of the peripheral wall of the valve core (6) and elastically abuts against the supporting wall (2113);

the end part of the valve rod (7) is provided with a mounting part (71) which extends along the axial direction of the valve rod (7) and has a diameter smaller than the diameter of the valve rod (7);

the clamping part (62), the sealing gasket (82) and the sealing part (61) are sequentially sleeved on the mounting part (71) along the direction away from the valve rod (7), and the sealing part (61) is in threaded connection with the end part of the mounting part (71).

8. The discharge assembly of a biological growth device of claim 7, wherein:

the feeding pipe (211) below the tank body is provided with a corner, the valve rod (7) extends along the axial direction of the feed port (5), penetrates through the pipe wall of the feeding pipe (211) from the corner and extends to the outside of the feeding pipe (211);

the lower part of the tank body is also provided with a driving component (9) which is connected with one end of the valve rod (7) far away from the valve core (6) and is used for driving the valve rod (7) to move along the axial direction of the feed port (5).

9. The discharge assembly of a biological growth device according to any one of claims 1-5, further comprising a cutting device for cutting the adventitious roots, wherein the valve core moves toward the interior of the tank after the cutting is completed to open the feed port and the adventitious roots are output from the feed port.

10. A discharge assembly of a biological growth device according to claim 9,

the cutting device comprises a cutting part (32) arranged in the cavity (90) of the tank body and a power part (34) capable of driving the cutting part (32);

the novel energy-saving tank is characterized by further comprising a spacer bush (31) arranged on the tank wall (11), wherein one side of the spacer bush (31) is connected with the shearing part (32), and the other side of the spacer bush is connected with the power part (34) and is used for connecting the shearing part and the power part.